名称: | pADH137 |
---|---|
别称: | Plasmid#90986 |
基因长: | |
质粒宿主: | 酵母菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | NAT |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AGGGTTATTG
TCTCATGAGC
GGATACATAT
TTGAATGTAT
TTAGAAAAAT
AAACAAATAG
GGGTTCCGCG
CACATTTCCC
CGAAAAGTGC
CACCTGACGT
CTAAGAAACC
ATTATTATCA
TGACATTAAC
CTATAAAAAT
AGGCGTATCA
CGAGGCCCTT
TGACGGCACG
GCCACGCGTT
TAAACCGCCA
TGTCTGTTAA
AACCAAAACC
ATTACTGTCT
TGCCTGGTGA
CCATGTCGGT
ACCGAAATTG
TCAATGAAGC
AATCAAAGTC
TTGAAAGCAA
TTGAAGCTGC
CACTCCCTAC
CAAAAAATCC
AATTTGATTT
CAAACACCAC
TTGATTGGTG
GTGCTGCCAT
TGATGCCACT
GGCGTCCCGC
TTCCAGATGA
TGCTCTTGAA
AGTGCTAAAT
CATCCGATGC
AGTTTTGTTG
GGTGCTGTTG
GTGGACCCAA
ATGGGGTACT
GGCACTGTTC
GTCCTGAACA
AGGATTATTG
AAAATCCGTA
AAGAATTGAA
CCTTTATGCC
AACATTAGAC
CATGTAATTT
TGCCAGTGAC
TCGTTATTAG
AATTGTCTCC
ATTAAAAGCT
GAAGTGGTCA
AAGGAACCAA
TTTAATTATT
GTTCGTGAAT
TAGTTGGTGG
TATATATTTT
GGTGAACGTC
AAGAACAAGA
AGAAAGTGAA
GACAAAAAAA
CAGCTTGGGA
TACAGAAAAG
TATACTGTTG
ACGAAGTTAC
TCGTATCACT
CGTATGGCAG
CATTTATGGC
TTTGCAGCAC
AATCCACCAT
TGCCAATATG
GTCTTTGGAC
AAAGCTAATG
TTTTGGCATC
GTCGAGATTG
TGGAGAAAGA
CCGTTGATAA
AGTGATTTCT
GAAGAATTTC
CAGCTTTGTC
TGTGCAGCAT
CAATTGATTG
ATTCGGCTGC
AATGATTTTG
ATCCAAAACC
CAACCAAATT
GAACGGTATC
ATCATTACAA
CAGCTAACAA
TTACACAAAA
AAAATTCTCT
TTTCGATGTA
GCAACTATCT
TCAACTAAAA
CATTTTCTCC
TTCGGCCCAT
GATTGTCCTC
CGGGTCGACA
GCAAGCCGTT
ACAATTGAGA
TGGAAAGCGA
CCTACCTTCA
CTCGATAAGG
TGCTTAATTG
TACTTCATAT
AAATCTGGCC
CGGATCTAAA
CAAATGAGTT
CCATTAAGCC
GTGGGTTCTC
AATTAGGGTT
TTTGTTTTTG
ATTTAGAAAA
AAGAGATCAA
GATTTGTTTA
CAGGTGATGC
CTTTTTTTAG
AACTTATGCG
TTGCAAAAGT
TGACTAACGA
TTTCTATAAG
GTGATCCACA
CTAATTATAC
AAACGTACAA
ACAGACATAC
TTTTCCTGCG
TTCACCTGAT
GTTGGCCAGA
TTTCTCTCTT
CATTGCATAG
AACATAACCA
CACTAGGGCA
ACAGAAAAAA
AAAAAAAAGT
GCATCGGGAA
GTTGTGTTCC
ATTCATTATA
TGTCTACTAC
TGCATATGAG
TAGCCCACCC
ACCACCACCA
TAGTAAGTTT
TTGTGTATGC
GCGCCGTCAG
GTTATTTCAT
TTCTGAATTT
TTCAACCACC
TTACTCCCTT
TATTGTTGAT
TGACAATTTT
GCTCACAGTA
AGATCTTTTA
GACTCCAATT
AATATAAAAT
AAGTCTGATT
TTCCAATTCC
TGTTTTTTCT
TTTTTTTTCT
GTTTCTATTT
CTTTCCTTTT
CTCCCTTTTT
TTTAATTCTT
CATTCAATCA
TCAATTGATA
ATTCAGGAAT
ATTACAACCC
GGGATGGATA
AAAAGTATAG
TATTGGTTTA
GATATTGGTA
CTAACTCTGT
GGGTTGGGCA
GTTATCACTG
ACGAATATAA
AGTTCCATCA
AAGAAATTTA
AGGTGTTAGG
TAACACTGAC
AGACACTCAA
TAAAAAAGAA
TCTTATCGGT
GCTCTTTTGT
TCGACTCCGG
TGAAACTGCC
GAGGCTACAC
GTTTAAAAAG
AACAGCAAGA
AGAAGATATA
CCCGTAGAAA
AAATAGAATA
TGTTATTTAC
AAGAAATCTT
TTCTAATGAA
ATGGCTAAAG
TTGATGATTC
CTTTTTCCAT
AGATTGGAAG
AGTCATTTTT
GGTTGAAGAA
GACAAAAAGC
ATGAGAGACA
TCCAATCTTT
GGGAATATAG
TTGATGAAGT
GGCTTACCAT
GAAAAATATC
CTACCATTTA
TCATTTAAGA
AAGAAATTGG
TAGATTCAAC
TGATAAAGCT
GACCTTAGAT
TAATCTATTT
AGCACTTGCC
CATATGATTA
AATTTAGAGG
TCATTTTTTG
ATTGAAGGTG
ATTTGAACCC
AGATAATTCT
GACGTGGATA
AATTATTTAT
TCAATTAGTC
CAAACCTACA
ACCAATTATT
TGAGGAAAAT
CCAATTAATG
CTAGTGGTGT
CGATGCCAAA
GCTATATTAT
CAGCCAGATT
ATCAAAATCT
AGACGTTTGG
AAAATTTGAT
TGCCCAATTG
CCAGGAGAAA
AAAAGAATGG
ATTATTTGGA
AACTTGATCG
CATTATCATT
GGGTTTGACA
CCAAATTTTA
AATCTAATTT
TGATTTAGCT
GAAGATGCTA
AATTACAATT
ATCAAAAGAC
ACCTATGACG
ACGATTTGGA
CAATTTACTT
GCTCAAATTG
GTGATCAATA
TGCAGATTTG
TTCTTAGCTG
CTAAAAACTT
ATCTGATGCT
ATTTTGTTGT
CTGATATTTT
GAGAGTGAAC
ACAGAAATAA
CCAAAGCTCC
ATTATCAGCA
TCTATGATCA
AACGTTATGA
TGAACACCAT
CAGGATTTGA
CTTTATTGAA
AGCTTTGGTG
AGACAACAAT
TGCCAGAGAA
GTATAAAGAA
ATCTTTTTCG
ATCAATCTAA
AAACGGGTAT
GCAGGTTATA
TTGATGGGGG
TGCCTCCCAA
GAGGAATTTT
ACAAATTTAT
AAAACCTATT
TTAGAAAAGA
TGGATGGGAC
TGAGGAACTT
TTGGTCAAAT
TGAACAGAGA
AGATTTGTTA
CGTAAACAGA
GAACTTTTGA
TAATGGTAGT
ATACCTCACC
AAATTCATTT
GGGTGAGTTG
CATGCAATTT
TAAGAAGACA
AGAAGATTTT
TATCCATTTT
TAAAAGATAA
TAGAGAAAAA
ATCGAGAAAA
TTTTAACCTT
TAGAATTCCA
TACTATGTTG
GGCCTTTGGC
TAGAGGTAAT
TCAAGATTTG
CCTGGATGAC
ACGTAAATCA
GAAGAAACTA
TTACCCCTTG
GAATTTTGAA
GAGGTTGTTG
ATAAAGGAGC
ATCAGCACAG
AGTTTTATTG
AAAGAATGAC
CAATTTCGAT
AAAAACTTAC
CAAATGAAAA
AGTTTTACCA
AAACATTCCT
TGTTATACGA
ATATTTTACT
GTTTACAATG
AACTTACAAA
GGTTAAATAT
GTTACTGAAG
GTATGCGTAA
GCCAGCCTTT
TTATCTGGAG
AACAGAAAAA
GGCAATAGTT
GATTTATTGT
TTAAAACAAA
TAGAAAAGTT
ACTGTTAAAC
AATTAAAAGA
AGATTACTTT
AAGAAAATTG
AATGTTTTGA
TTCAGTTGAA
ATCAGTGGTG
TTGAAGACAG
ATTTAATGCT
AGTTTAGGAA
CTTACCATGA
TTTACTTAAA
ATTATCAAAG
ATAAAGATTT
CTTGGATAAC
GAAGAAAATG
AAGACATTTT
AGAAGACATT
GTTTTAACCT
TAACTTTATT
CGAAGATAGA
GAGATGATTG
AAGAACGTTT
GAAGACTTAT
GCACATTTGT
TTGACGATAA
AGTGATGAAA
CAGTTGAAAA
GAAGACGTTA
TACTGGATGG
GGTAGATTGT
CTCGTAAATT
GATCAATGGA
ATTAGAGATA
AACAAAGTGG
TAAAACTATC
TTGGACTTTT
TGAAATCTGA
CGGATTTGCT
AATAGAAATT
TCATGCAATT
GATCCACGAC
GATAGTTTGA
CATTTAAAGA
AGACATCCAA
AAGGCCCAAG
TGAGTGGGCA
AGGTGATTCA
TTACATGAAC
ATATTGCAAA
TTTAGCCGGA
TCTCCTGCTA
TTAAGAAAGG
GATATTACAA
ACTGTTAAAG
TTGTGGATGA
ATTAGTGAAA
GTAATGGGAA
GACATAAACC
TGAAAACATT
GTCATTGAGA
TGGCAAGAGA
AAATCAAACT
ACACAAAAAG
GACAGAAAAA
TAGTAGAGAA
CGTATGAAAA
GAATAGAAGA
GGGTATTAAA
GAATTGGGTA
GTCAAATATT
GAAAGAACAC
CCAGTGGAAA
ATACCCAGTT
GCAAAATGAA
AAATTATATC
TTTACTACCT
TCAAAATGGA
CGTGATATGT
ATGTTGATCA
GGAATTAGAT
ATAAATAGAC
TTTCAGATTA
TGATGTAGAT
CATATAGTTC
CACAATCTTT
CTTGAAAGAT
GATTCCATAG
ACAATAAAGT
ATTAACTAGA
AGTGATAAAA
ATAGAGGTAA
AAGTGATAAT
GTCCCAAGTG
AGGAAGTCGT
CAAAAAGATG
AAAAATTACT
GGCGTCAACT
TTTGAATGCT
AAATTAATTA
CTCAAAGAAA
ATTTGATAAT
TTGACTAAAG
CAGAAAGAGG
TGGGCTTTCT
GAATTAGATA
AAGCCGGGTT
CATTAAAAGA
CAATTGGTCG
AAACTAGACA
AATTACTAAA
CATGTTGCCC
AAATTTTAGA
TTCCCGTATG
AACACTAAGT
ATGACGAAAA
TGATAAGTTA
ATACGTGAGG
TTAAAGTCAT
TACTTTAAAA
TCAAAACTTG
TCTCTGATTT
CAGAAAGGAT
TTCCAATTCT
ATAAAGTTAG
AGAAATTAAT
AATTATCATC
ATGCTCATGA
TGCATATTTG
AATGCTGTAG
TTGGAACTGC
TTTAATCAAG
AAATACCCTA
AATTAGAATC
TGAATTTGTA
TATGGTGATT
ACAAAGTCTA
TGATGTTAGA
AAGATGATTG
CTAAATCAGA
ACAAGAAATT
GGTAAAGCTA
CAGCTAAATA
CTTCTTTTAC
TCTAACATTA
TGAATTTCTT
TAAAACAGAA
ATTACTTTGG
CAAACGGTGA
AATTAGAAAA
AGACCTCTTA
TTGAAACAAA
TGGTGAGACT
GGAGAGATAG
TTTGGGACAA
AGGGCGTGAT
TTCGCTACTG
TTAGAAAAGT
TTTATCAATG
CCACAAGTTA
ACATTGTAAA
GAAAACAGAG
GTTCAAACTG
GTGGTTTCTC
AAAAGAAAGT
ATTTTGCCTA
AAAGAAATAG
TGATAAATTG
ATTGCCAGAA
AAAAGGATTG
GGATCCAAAG
AAATATGGTG
GTTTCGACTC
ACCAACCGTA
GCCTATTCTG
TTTTGGTTGT
GGCAAAGGTT
GAAAAGGGTA
AAAGTAAAAA
GCTTAAATCA
GTAAAAGAAC
TTTTGGGTAT
TACAATAATG
GAAAGAAGTT
CCTTTGAAAA
GAACCCTATT
GATTTTTTGG
AAGCTAAAGG
TTATAAGGAA
GTAAAGAAGG
ACTTAATAAT
CAAATTGCCT
AAATATTCTT
TATTTGAATT
AGAAAATGGG
AGAAAAAGAA
TGTTGGCTTC
TGCTGGAGAA
TTGCAAAAGG
GTAATGAATT
AGCATTGCCT
TCCAAATATG
TTAACTTCTT
GTATTTAGCT
TCACACTATG
AAAAGTTGAA
AGGGTCACCA
GAAGATAACG
AGCAAAAACA
ATTATTTGTT
GAACAACACA
AACACTACTT
AGATGAGATT
ATAGAACAAA
TTAGTGAATT
CAGTAAAAGA
GTGATATTAG
CTGATGCAAA
TTTAGATAAA
GTTTTGTCAG
CCTATAACAA
ACATAGAGAT
AAGCCAATTA
GAGAACAAGC
AGAAAACATT
ATTCACTTAT
TTACCCTTAC
CAATTTAGGA
GCACCTGCTG
CTTTCAAGTA
TTTTGATACA
ACAATTGATC
GTAAAAGATA
TACCTCAACA
AAAGAAGTCT
TAGACGCCAC
CTTAATTCAT
CAATCAATCA
CTGGATTGTA
TGAGACAAGA
ATTGATTTGT
CTCAATTGGG
TGGTGATGAA
GGGGCTGATC
CTAAGAAGAA
AAGAAAAGTT
GATCCAAAGA
AAAAGCGTAA
GGTGGATCCT
AAGAAAAAGA
GAAAGGTTGA
CTACAAAGAC
CATGACGGTG
ATTATAAAGA
TCATGACATC
GACTACAAGG
ATGACGATGA
CAAGTGATAA
TGACTGCAGA
GATCCATCGA
CCTGCCGCCA
AGCTAATTCC
GGGCGAATTT
CTGTCGAGTC
ATGTAATTAG
TTATGTCACG
CTTACATTCA
CGCCCTCCCC
CCACATCCGC
TCTAACCGAA
AAGGAAGGAG
TTAGACAACC
TGAAGTCTAG
GTCCCTATTT
ATTTTTTTAT
AGTTATGTTA
GTATTAAGAA
CGTTATTTAT
ATTTCAAATT
TTTCTTTTTT
TTCTGTACAG
ACGCGTGTAC
GCATGTAACA
TTATACTGAA
AACCTTGCTT
TAGAAGGTTT
TGGGACGCTC
GAAGGCTTTA
ATTTGCGGCC
AGCTTGCCTC
GTCCCCGCCT
GGTCACCCGG
CCAGCGACAT
GGAGGCCCAG
AATACCCTCC
TTGACAGTCT
TGACGTGCGC
AGCTCAGGGG
CATGATGTGA
CTGTCGCCCG
TACATTTAGC
CCATACATCC
CCATGTATAA
TCATTTGCAT
CCATACATTT
TGATGGCCGC
ACGGCGCGAA
GCAAAAATTA
CGGCTCCTCG
CTGCAGACCT
GCGAGCAGGG
AAACGCTCCC
CTCACAGACG
CGTTGAATTG
TCCCCACGCC
GCGCCCCTGT
AGAGAAATAT
AAAAGGTTAG
GATTTGCCAC
TGAGGTTCTT
CTTTCATATA
CTTCCTTTTA
AAATCTTGCT
AGGATACAGT
TCTCACATCA
CATCCGAACA
TAAACAACCG
GATCCTACAA
TTATGTCTAC
TACTTTGGAT
GATACTGCTT
ATAGATACAG
AACTTCTGTT
CCAGGTGATG
CTGAAGCTAT
TGAAGCTTTG
GATGGTTCTT
TCACTACCGA
TACTGTTTTT
AGAGTTACTG
CTACTGGTGA
TGGTTTCACT
TTGAGAGAAG
TTCCAGTTGA
TCCACCATTG
ACTAAGGTTT
TCCCAGATGA
TGAATCCGAT
GATGAATCCG
ATGCTGGTGA
AGATGGTGAT
CCAGATTCTA
GAACTTTCGT
TGCTTATGGT
GATGATGGTG
ATTTGGCTGG
TTTCGTTGTT
GTTTCTTATT
CTGGTTGGAA
CAGAAGATTG
ACTGTTGAAG
ATATTGAAGT
TGCTCCAGAA
CATAGAGGTC
ATGGTGTTGG
TAGAGCTTTG
ATGGGTTTGG
CTACTGAATT
CGCCAGAGAA
AGAGGTGCTG
GTCATTTGTG
GTTGGAAGTT
ACCAATGTTA
ATCGGTGTTT
AAACCCCAGC
GCCTGGCGGG
CGCTCACAAT
TCCACACAAC
ATACGAGCCG
GAAGCATAAA
GTGTAAAGCC
TGGGGTGCCT
AATGAGTGAG
CTAACTCACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GAACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TGATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATC