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G1397 DddAtox-C–SaKKH-Cas9(D10A)

产品货号:P48696

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: G1397 DddAtox-C–SaKKH-Cas9(D10A)
别称: Plasmid#157840
基因长:
质粒宿主: 哺乳细胞
质粒用途: 蛋白表达
片段类型: ORF,CRISPR
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

ATATGCCAAG

TACGCCCCCT

ATTGACGTCA

ATGACGGTAA

ATGGCCCGCC

TGGCATTATG

61

CCCAGTACAT

GACCTTATGG

GACTTTCCTA

CTTGGCAGTA

CATCTACGTA

TTAGTCATCG

121

CTATTACCAT

GGTGATGCGG

TTTTGGCAGT

ACATCAATGG

GCGTGGATAG

CGGTTTGACT

181

CACGGGGATT

TCCAAGTCTC

CACCCCATTG

ACGTCAATGG

GAGTTTGTTT

TGGCACCAAA

241

ATCAACGGGA

CTTTCCAAAA

TGTCGTAACA

ACTCCGCCCC

ATTGACGCAA

ATGGGCGGTA

301

GGCGTGTACG

GTGGGAGGTC

TATATAAGCA

GAGCTGGTTT

AGTGAACCGT

CAGATCCGCT

361

AGAGATCCGC

GGCCGCTAAT

ACGACTCACT

ATAGGGAGAG

CCGCCACCAT

GGCCATTCCA

421

GTGAAGCGCG

GCGCTACCGG

TGAAACCAAA

GTGTTTACCG

GTAACAGCAA

CAGCCCGAAG

481

AGCCCGACCA

AAGGCGGTTG

CTCTGGTGGT

TCTTCTGGTG

GTTCTAGCGG

CAGCGAGACT

541

CCCGGGACCT

CAGAGTCCGC

CACACCCGAA

AGTAGTGGCG

GCAGCAGCGG

CGGCAGCGGG

601

AAACGGAACT

ACATCCTGGG

GCTTGCCATT

GGGATAACCA

GCGTTGGCTA

CGGAATTATT

661

GATTATGAGA

CACGCGATGT

GATTGACGCC

GGGGTTAGGC

TGTTCAAAGA

GGCCAACGTT

721

GAAAACAACG

AGGGAAGACG

GAGTAAGCGC

GGAGCAAGAA

GACTCAAGCG

CAGACGGAGA

781

CATCGGATTC

AGAGGGTGAA

AAAGCTGCTC

TTCGATTACA

ATCTCCTGAC

CGATCATAGT

841

GAGCTGAGCG

GAATCAACCC

CTACGAGGCG

CGAGTGAAAG

GGCTTTCCCA

GAAGCTGTCC

901

GAAGAGGAGT

TCTCCGCCGC

GTTGCTGCAC

CTGGCCAAAC

GGAGGGGGGT

TCACAATGTA

961

AACGAAGTGG

AGGAGGACAC

GGGCAATGAA

CTTAGTACGA

AAGAACAGAT

CAGTAGGAAC

1021

TCTAAGGCTC

TCGAAGAGAA

ATACGTCGCT

GAGTTGCAGC

TTGAGAGACT

GAAAAAAGAC

1081

GGCGAAGTAC

GCGGATCTAT

TAATAGGTTC

AAGACTTCAG

ATTACGTAAA

GGAAGCCAAG

1141

CAGCTCCTGA

AAGTACAGAA

AGCGTACCAT

CAGCTCGATC

AGAGCTTCAT

CGATACCTAC

1201

ATAGATTTGC

TGGAGACACG

GAGGACATAC

TACGAGGGCC

CAGGGGAAGG

ATCTCCTTTT

1261

GGGTGGAAGG

ACATCAAGGA

ATGGTACGAG

ATGCTTATGG

GACATTGTAC

ATATTTTCCG

1321

GAGGAGCTCA

GGAGCGTCAA

GTACGCCTAC

AATGCCGACC

TGTACAATGC

CCTCAATGAC

1381

CTCAATAACC

TCGTGATTAC

CAGGGACGAG

AACGAGAAGC

TGGAGTACTA

TGAAAAGTTC

1441

CAGATTATCG

AGAATGTGTT

TAAGCAGAAG

AAGAAGCCGA

CACTTAAGCA

GATTGCAAAG

1501

GAAATCCTCG

TGAATGAGGA

AGATATCAAG

GGATACAGAG

TGACAAGTAC

AGGCAAGCCC

1561

GAGTTCACAA

ATCTGAAGGT

GTACCACGAT

ATTAAGGACA

TAACCGCACG

AAAGGAGATA

1621

ATCGAAAACG

CTGAGCTCCT

CGATCAGATC

GCAAAAATTC

TTACCATCTA

CCAGTCTAGT

1681

GAGGACATTC

AGGAGGAACT

GACTAATCTG

AACAGTGAGC

TCACCCAAGA

GGAAATTGAG

1741

CAGATTTCAA

ACCTGAAAGG

CTACACCGGG

ACGCACAATC

TGAGCCTCAA

AGCAATCAAC

1801

CTCATTCTGG

ATGAACTTTG

GCACACAAAT

GACAACCAAA

TTGCCATATT

CAACCGCCTG

1861

AAACTGGTGC

CAAAAAAAGT

GGATCTGTCA

CAGCAAAAGG

AAATCCCTAC

AACCTTGGTT

1921

GACGATTTTA

TTCTGTCCCC

CGTTGTCAAG

CGGAGCTTCA

TCCAGTCAAT

CAAGGTGATC

1981

AATGCCATCA

TTAAAAAATA

CGGATTGCCA

AACGATATAA

TTATCGAGCT

TGCACGAGAG

2041

AAGAACTCAA

AGGACGCCCA

GAAGATGATT

AACGAAATGC

AGAAGCGCAA

CCGCCAGACA

2101

AACGAACGCA

TAGAGGAAAT

TATAAGAACA

ACCGGCAAAG

AGAATGCCAA

GTATCTGATC

2161

GAGAAAATCA

AGCTGCACGA

CATGCAAGAA

GGCAAGTGCC

TGTACTCTCT

GGAAGCTATC

2221

CCACTCGAAG

ATCTGCTGAA

TAATCCATTC

AATTACGAGG

TGGACCACAT

CATCCCTAGA

2281

TCCGTAAGCT

TTGACAATTC

CTTCAATAAC

AAAGTTCTGG

TTAAACAGGA

GGAAAATTCT

2341

AAAAAAGGGA

ACCGGACCCC

GTTCCAGTAC

CTGAGCTCCA

GTGACAGCAA

GATTAGCTAC

2401

GAGACTTTTA

AGAAACATAT

TCTGAATCTG

GCCAAAGGCA

AAGGCAGGAT

CAGCAAGACC

2461

AAGAAGGAGT

ACCTCCTCGA

AGAACGCGAC

ATTAACAGAT

TTAGTGTGCA

GAAAGATTTC

2521

ATCAACCGAA

ACCTTGTCGA

TACTCGGTAC

GCCACGAGAG

GCCTGATGAA

TCTCCTCAGG

2581

AGCTACTTCC

GCGTCAATAA

TCTGGACGTT

AAAGTCAAGA

GCATAAATGG

GGGATTCACC

2641

AGCTTTCTGA

GGAGAAAGTG

GAAGTTTAAG

AAGGAACGAA

ACAAAGGATA

CAAGCACCAT

2701

GCTGAGGATG

CTTTGATCAT

CGCTAACGCG

GACTTTATCT

TTAAGGAATG

GAAAAAGCTG

2761

GATAAGGCAA

AGAAAGTGAT

GGAAAACCAG

ATGTTCGAGG

AGAAGCAGGC

AGAGTCAATG

2821

CCTGAGATCG

AGACAGAGCA

GGAATACAAG

GAAATTTTCA

TCACCCCTCA

TCAGATTAAA

2881

CACATAAAGG

ACTTCAAAGA

CTATAAATAC

TCTCATAGGG

TGGACAAAAA

ACCCAATCGC

2941

AAGCTCATTA

ATGACACCCT

GTACTCAACA

CGGAAGGATG

ATAAAGGTAA

TACCTTGATT

3001

GTGAATAATC

TTAATGGATT

GTATGACAAA

GATAACGACA

AGCTCAAGAA

GCTGATCAAC

3061

AAGTCTCCAG

AGAAGCTCCT

TATGTATCAC

CACGACCCAC

AGACTTATCA

GAAATTGAAA

3121

CTGATCATGG

AGCAATACGG

GGATGAGAAG

AACCCACTCT

ACAAATATTA

TGAGGAAACA

3181

GGTAATTACC

TGACCAAGTA

CTCCAAGAAG

GATAACGGAC

CAGTGATCAA

AAAGATAAAG

3241

TACTATGGCA

ACAAACTTAA

TGCGCATTTG

GACATAACTG

ACGATTACCC

CAATTCTCGA

3301

AACAAGGTTG

TGAAGCTCTC

CCTGAAGCCT

TATAGATTTG

ACGTGTACCT

GGATAATGGG

3361

GTTTATAAAT

TCGTCACCGT

GAAAAATCTG

GACGTGATCA

AAAAGGAGAA

CTATTATGAA

3421

GTAAACTCAA

AGTGCTATGA

GGAGGCGAAG

AAGCTGAAGA

AGATCTCCAA

TCAGGCCGAG

3481

TTCATCGCTT

CCTTCTATAA

GAACGATCTC

ATCAAGATCA

ATGGAGAGCT

TTATCGCGTC

3541

ATTGGTGTGA

ACAATGACTT

GCTGAACAGG

ATCGAAGTCA

ATATGATAGA

CATTACCTAC

3601

CGGGAGTATC

TCGAAAACAT

GAATGATAAA

CGGCCGCCTC

ACATCATCAA

GACAATCGCA

3661

TCTAAAACTC

AGTCAATAAA

AAAGTACTCT

ACCGATATCC

TGGGGAATCT

CTATGAAGTG

3721

AAGTCAAAGA

AGCACCCACA

AATCATTAAA

AAAGGTGGAT

CCCCCAAGAA

GAAGAGGAAA

3781

GTCTCGAGCG

ACTACAAAGA

CCATGACGGT

GATTATAAAG

ATCATGACAT

CGATTACAAG

3841

GATGACGATG

ACAAGTCTGG

TGGTTCTACT

AATCTGTCAG

ATATTATTGA

AAAGGAGACC

3901

GGTAAGCAAC

TGGTTATCCA

GGAATCCATC

CTCATGCTCC

CAGAGGAGGT

GGAAGAAGTC

3961

ATTGGGAACA

AGCCGGAAAG

CGATATACTC

GTGCACACCG

CCTACGACGA

GAGCACCGAC

4021

GAGAATGTCA

TGCTTCTGAC

TAGCGACGCC

CCTGAATACA

AGCCTTGGGC

TCTGGTCATA

4081

CAGGATAGCA

ACGGTGAGAA

CAAGATTAAG

ATGCTCTCTG

GTGGTTCTCC

CAAGAAGAAG

4141

AGGAAAGTCT

AACCGGTCAT

CATCACCATC

ACCATTGAGT

TTAAACCCGC

TGATCAGCCT

4201

CGACTGTGCC

TTCTAGTTGC

CAGCCATCTG

TTGTTTGCCC

CTCCCCCGTG

CCTTCCTTGA

4261

CCCTGGAAGG

TGCCACTCCC

ACTGTCCTTT

CCTAATAAAA

TGAGGAAATT

GCATCGCATT

4321

GTCTGAGTAG

GTGTCATTCT

ATTCTGGGGG

GTGGGGTGGG

GCAGGACAGC

AAGGGGGAGG

4381

ATTGGGAAGA

CAATAGCAGG

CATGCTGGGG

ATGCGGTGGG

CTCTATGGCT

TCTGAGGCGG

4441

AAAGAACCAG

CTGGGGCTCG

ATACCGTCGA

CCTCTAGCTA

GAGCTTGGCG

TAATCATGGT

4501

CATAGCTGTT

TCCTGTGTGA

AATTGTTATC

CGCTCACAAT

TCCACACAAC

ATACGAGCCG

4561

GAAGCATAAA

GTGTAAAGCC

TAGGGTGCCT

AATGAGTGAG

CTAACTCACA

TTAATTGCGT

4621

TGCGCTCACT

GCCCGCTTTC

CAGTCGGGAA

ACCTGTCGTG

CCAGCTGCAT

TAATGAATCG

4681

GCCAACGCGC

GGGGAGAGGC

GGTTTGCGTA

TTGGGCGCTC

TTCCGCTTCC

TCGCTCACTG

4741

ACTCGCTGCG

CTCGGTCGTT

CGGCTGCGGC

GAGCGGTATC

AGCTCACTCA

AAGGCGGTAA

4801

TACGGTTATC

CACAGAATCA

GGGGATAACG

CAGGAAAGAA

CATGTGAGCA

AAAGGCCAGC

4861

AAAAGGCCAG

GAACCGTAAA

AAGGCCGCGT

TGCTGGCGTT

TTTCCATAGG

CTCCGCCCCC

4921

CTGACGAGCA

TCACAAAAAT

CGACGCTCAA

GTCAGAGGTG

GCGAAACCCG

ACAGGACTAT

4981

AAAGATACCA

GGCGTTTCCC

CCTGGAAGCT

CCCTCGTGCG

CTCTCCTGTT

CCGACCCTGC

5041

CGCTTACCGG

ATACCTGTCC

GCCTTTCTCC

CTTCGGGAAG

CGTGGCGCTT

TCTCATAGCT

5101

CACGCTGTAG

GTATCTCAGT

TCGGTGTAGG

TCGTTCGCTC

CAAGCTGGGC

TGTGTGCACG

5161

AACCCCCCGT

TCAGCCCGAC

CGCTGCGCCT

TATCCGGTAA

CTATCGTCTT

GAGTCCAACC

5221

CGGTAAGACA

CGACTTATCG

CCACTGGCAG

CAGCCACTGG

TAACAGGATT

AGCAGAGCGA

5281

GGTATGTAGG

CGGTGCTACA

GAGTTCTTGA

AGTGGTGGCC

TAACTACGGC

TACACTAGAA

5341

GAACAGTATT

TGGTATCTGC

GCTCTGCTGA

AGCCAGTTAC

CTTCGGAAAA

AGAGTTGGTA

5401

GCTCTTGATC

CGGCAAACAA

ACCACCGCTG

GTAGCGGTGG

TTTTTTTGTT

TGCAAGCAGC

5461

AGATTACGCG

CAGAAAAAAA

GGATCTCAAG

AAGATCCTTT

GATCTTTTCT

ACGGGGTCTG

5521

ACGCTCAGTG

GAACGAAAAC

TCACGTTAAG

GGATTTTGGT

CATGAGATTA

TCAAAAAGGA

5581

TCTTCACCTA

GATCCTTTTA

AATTAAAAAT

GAAGTTTTAA

ATCAATCTAA

AGTATATATG

5641

AGTAAACTTG

GTCTGACAGT

TACCAATGCT

TAATCAGTGA

GGCACCTATC

TCAGCGATCT

5701

GTCTATTTCG

TTCATCCATA

GTTGCCTGAC

TCCCCGTCGT

GTAGATAACT

ACGATACGGG

5761

AGGGCTTACC

ATCTGGCCCC

AGTGCTGCAA

TGATACCGCG

AGACCCACGC

TCACCGGCTC

5821

CAGATTTATC

AGCAATAAAC

CAGCCAGCCG

GAAGGGCCGA

GCGCAGAAGT

GGTCCTGCAA

5881

CTTTATCCGC

CTCCATCCAG

TCTATTAATT

GTTGCCGGGA

AGCTAGAGTA

AGTAGTTCGC

5941

CAGTTAATAG

TTTGCGCAAC

GTTGTTGCCA

TTGCTACAGG

CATCGTGGTG

TCACGCTCGT

6001

CGTTTGGTAT

GGCTTCATTC

AGCTCCGGTT

CCCAACGATC

AAGGCGAGTT

ACATGATCCC

6061

CCATGTTGTG

CAAAAAAGCG

GTTAGCTCCT

TCGGTCCTCC

GATCGTTGTC

AGAAGTAAGT

6121

TGGCCGCAGT

GTTATCACTC

ATGGTTATGG

CAGCACTGCA

TAATTCTCTT

ACTGTCATGC

6181

CATCCGTAAG

ATGCTTTTCT

GTGACTGGTG

AGTACTCAAC

CAAGTCATTC

TGAGAATAGT

6241

GTATGCGGCG

ACCGAGTTGC

TCTTGCCCGG

CGTCAATACG

GGATAATACC

GCGCCACATA

6301

GCAGAACTTT

AAAAGTGCTC

ATCATTGGAA

AACGTTCTTC

GGGGCGAAAA

CTCTCAAGGA

6361

TCTTACCGCT

GTTGAGATCC

AGTTCGATGT

AACCCACTCG

TGCACCCAAC

TGATCTTCAG

6421

CATCTTTTAC

TTTCACCAGC

GTTTCTGGGT

GAGCAAAAAC

AGGAAGGCAA

AATGCCGCAA

6481

AAAAGGGAAT

AAGGGCGACA

CGGAAATGTT

GAATACTCAT

ACTCTTCCTT

TTTCAATATT

6541

ATTGAAGCAT

TTATCAGGGT

TATTGTCTCA

TGAGCGGATA

CATATTTGAA

TGTATTTAGA

6601

AAAATAAACA

AATAGGGGTT

CCGCGCACAT

TTCCCCGAAA

AGTGCCACCT

GACGTCGACG

6661

GATCGGGAGA

TCGATCTCCC

GATCCCCTAG

GGTCGACTCT

CAGTACAATC

TGCTCTGATG

6721

CCGCATAGTT

AAGCCAGTAT

CTGCTCCCTG

CTTGTGTGTT

GGAGGTCGCT

GAGTAGTGCG

6781

CGAGCAAAAT

TTAAGCTACA

ACAAGGCAAG

GCTTGACCGA

CAATTGCATG

AAGAATCTGC

6841

TTAGGGTTAG

GCGTTTTGCG

CTGCTTCGCG

ATGTACGGGC

CAGATATACG

CGTTGACATT

6901

GATTATTGAC

TAGTTATTAA

TAGTAATCAA

TTACGGGGTC

ATTAGTTCAT

AGCCCATATA

6961

TGGAGTTCCG

CGTTACATAA

CTTACGGTAA

ATGGCCCGCC

TGGCTGACCG

CCCAACGACC

7021

CCCGCCCATT

GACGTCAATA

ATGACGTATG

TTCCCATAGT

AACGCCAATA

GGGACTTTCC

7081

ATTGACGTCA

ATGGGTGGAG

TATTTACGGT

AAACTGCCCA

CTTGGCAGTA

CATCAAGTGT

7141

ATC

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