名称: | G1397 DddAtox-C–SaKKH-Cas9(D10A) |
---|---|
别称: | Plasmid#157840 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF,CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GAGCTGGTTT
AGTGAACCGT
CAGATCCGCT
AGAGATCCGC
GGCCGCTAAT
ACGACTCACT
ATAGGGAGAG
CCGCCACCAT
GGCCATTCCA
GTGAAGCGCG
GCGCTACCGG
TGAAACCAAA
GTGTTTACCG
GTAACAGCAA
CAGCCCGAAG
AGCCCGACCA
AAGGCGGTTG
CTCTGGTGGT
TCTTCTGGTG
GTTCTAGCGG
CAGCGAGACT
CCCGGGACCT
CAGAGTCCGC
CACACCCGAA
AGTAGTGGCG
GCAGCAGCGG
CGGCAGCGGG
AAACGGAACT
ACATCCTGGG
GCTTGCCATT
GGGATAACCA
GCGTTGGCTA
CGGAATTATT
GATTATGAGA
CACGCGATGT
GATTGACGCC
GGGGTTAGGC
TGTTCAAAGA
GGCCAACGTT
GAAAACAACG
AGGGAAGACG
GAGTAAGCGC
GGAGCAAGAA
GACTCAAGCG
CAGACGGAGA
CATCGGATTC
AGAGGGTGAA
AAAGCTGCTC
TTCGATTACA
ATCTCCTGAC
CGATCATAGT
GAGCTGAGCG
GAATCAACCC
CTACGAGGCG
CGAGTGAAAG
GGCTTTCCCA
GAAGCTGTCC
GAAGAGGAGT
TCTCCGCCGC
GTTGCTGCAC
CTGGCCAAAC
GGAGGGGGGT
TCACAATGTA
AACGAAGTGG
AGGAGGACAC
GGGCAATGAA
CTTAGTACGA
AAGAACAGAT
CAGTAGGAAC
TCTAAGGCTC
TCGAAGAGAA
ATACGTCGCT
GAGTTGCAGC
TTGAGAGACT
GAAAAAAGAC
GGCGAAGTAC
GCGGATCTAT
TAATAGGTTC
AAGACTTCAG
ATTACGTAAA
GGAAGCCAAG
CAGCTCCTGA
AAGTACAGAA
AGCGTACCAT
CAGCTCGATC
AGAGCTTCAT
CGATACCTAC
ATAGATTTGC
TGGAGACACG
GAGGACATAC
TACGAGGGCC
CAGGGGAAGG
ATCTCCTTTT
GGGTGGAAGG
ACATCAAGGA
ATGGTACGAG
ATGCTTATGG
GACATTGTAC
ATATTTTCCG
GAGGAGCTCA
GGAGCGTCAA
GTACGCCTAC
AATGCCGACC
TGTACAATGC
CCTCAATGAC
CTCAATAACC
TCGTGATTAC
CAGGGACGAG
AACGAGAAGC
TGGAGTACTA
TGAAAAGTTC
CAGATTATCG
AGAATGTGTT
TAAGCAGAAG
AAGAAGCCGA
CACTTAAGCA
GATTGCAAAG
GAAATCCTCG
TGAATGAGGA
AGATATCAAG
GGATACAGAG
TGACAAGTAC
AGGCAAGCCC
GAGTTCACAA
ATCTGAAGGT
GTACCACGAT
ATTAAGGACA
TAACCGCACG
AAAGGAGATA
ATCGAAAACG
CTGAGCTCCT
CGATCAGATC
GCAAAAATTC
TTACCATCTA
CCAGTCTAGT
GAGGACATTC
AGGAGGAACT
GACTAATCTG
AACAGTGAGC
TCACCCAAGA
GGAAATTGAG
CAGATTTCAA
ACCTGAAAGG
CTACACCGGG
ACGCACAATC
TGAGCCTCAA
AGCAATCAAC
CTCATTCTGG
ATGAACTTTG
GCACACAAAT
GACAACCAAA
TTGCCATATT
CAACCGCCTG
AAACTGGTGC
CAAAAAAAGT
GGATCTGTCA
CAGCAAAAGG
AAATCCCTAC
AACCTTGGTT
GACGATTTTA
TTCTGTCCCC
CGTTGTCAAG
CGGAGCTTCA
TCCAGTCAAT
CAAGGTGATC
AATGCCATCA
TTAAAAAATA
CGGATTGCCA
AACGATATAA
TTATCGAGCT
TGCACGAGAG
AAGAACTCAA
AGGACGCCCA
GAAGATGATT
AACGAAATGC
AGAAGCGCAA
CCGCCAGACA
AACGAACGCA
TAGAGGAAAT
TATAAGAACA
ACCGGCAAAG
AGAATGCCAA
GTATCTGATC
GAGAAAATCA
AGCTGCACGA
CATGCAAGAA
GGCAAGTGCC
TGTACTCTCT
GGAAGCTATC
CCACTCGAAG
ATCTGCTGAA
TAATCCATTC
AATTACGAGG
TGGACCACAT
CATCCCTAGA
TCCGTAAGCT
TTGACAATTC
CTTCAATAAC
AAAGTTCTGG
TTAAACAGGA
GGAAAATTCT
AAAAAAGGGA
ACCGGACCCC
GTTCCAGTAC
CTGAGCTCCA
GTGACAGCAA
GATTAGCTAC
GAGACTTTTA
AGAAACATAT
TCTGAATCTG
GCCAAAGGCA
AAGGCAGGAT
CAGCAAGACC
AAGAAGGAGT
ACCTCCTCGA
AGAACGCGAC
ATTAACAGAT
TTAGTGTGCA
GAAAGATTTC
ATCAACCGAA
ACCTTGTCGA
TACTCGGTAC
GCCACGAGAG
GCCTGATGAA
TCTCCTCAGG
AGCTACTTCC
GCGTCAATAA
TCTGGACGTT
AAAGTCAAGA
GCATAAATGG
GGGATTCACC
AGCTTTCTGA
GGAGAAAGTG
GAAGTTTAAG
AAGGAACGAA
ACAAAGGATA
CAAGCACCAT
GCTGAGGATG
CTTTGATCAT
CGCTAACGCG
GACTTTATCT
TTAAGGAATG
GAAAAAGCTG
GATAAGGCAA
AGAAAGTGAT
GGAAAACCAG
ATGTTCGAGG
AGAAGCAGGC
AGAGTCAATG
CCTGAGATCG
AGACAGAGCA
GGAATACAAG
GAAATTTTCA
TCACCCCTCA
TCAGATTAAA
CACATAAAGG
ACTTCAAAGA
CTATAAATAC
TCTCATAGGG
TGGACAAAAA
ACCCAATCGC
AAGCTCATTA
ATGACACCCT
GTACTCAACA
CGGAAGGATG
ATAAAGGTAA
TACCTTGATT
GTGAATAATC
TTAATGGATT
GTATGACAAA
GATAACGACA
AGCTCAAGAA
GCTGATCAAC
AAGTCTCCAG
AGAAGCTCCT
TATGTATCAC
CACGACCCAC
AGACTTATCA
GAAATTGAAA
CTGATCATGG
AGCAATACGG
GGATGAGAAG
AACCCACTCT
ACAAATATTA
TGAGGAAACA
GGTAATTACC
TGACCAAGTA
CTCCAAGAAG
GATAACGGAC
CAGTGATCAA
AAAGATAAAG
TACTATGGCA
ACAAACTTAA
TGCGCATTTG
GACATAACTG
ACGATTACCC
CAATTCTCGA
AACAAGGTTG
TGAAGCTCTC
CCTGAAGCCT
TATAGATTTG
ACGTGTACCT
GGATAATGGG
GTTTATAAAT
TCGTCACCGT
GAAAAATCTG
GACGTGATCA
AAAAGGAGAA
CTATTATGAA
GTAAACTCAA
AGTGCTATGA
GGAGGCGAAG
AAGCTGAAGA
AGATCTCCAA
TCAGGCCGAG
TTCATCGCTT
CCTTCTATAA
GAACGATCTC
ATCAAGATCA
ATGGAGAGCT
TTATCGCGTC
ATTGGTGTGA
ACAATGACTT
GCTGAACAGG
ATCGAAGTCA
ATATGATAGA
CATTACCTAC
CGGGAGTATC
TCGAAAACAT
GAATGATAAA
CGGCCGCCTC
ACATCATCAA
GACAATCGCA
TCTAAAACTC
AGTCAATAAA
AAAGTACTCT
ACCGATATCC
TGGGGAATCT
CTATGAAGTG
AAGTCAAAGA
AGCACCCACA
AATCATTAAA
AAAGGTGGAT
CCCCCAAGAA
GAAGAGGAAA
GTCTCGAGCG
ACTACAAAGA
CCATGACGGT
GATTATAAAG
ATCATGACAT
CGATTACAAG
GATGACGATG
ACAAGTCTGG
TGGTTCTACT
AATCTGTCAG
ATATTATTGA
AAAGGAGACC
GGTAAGCAAC
TGGTTATCCA
GGAATCCATC
CTCATGCTCC
CAGAGGAGGT
GGAAGAAGTC
ATTGGGAACA
AGCCGGAAAG
CGATATACTC
GTGCACACCG
CCTACGACGA
GAGCACCGAC
GAGAATGTCA
TGCTTCTGAC
TAGCGACGCC
CCTGAATACA
AGCCTTGGGC
TCTGGTCATA
CAGGATAGCA
ACGGTGAGAA
CAAGATTAAG
ATGCTCTCTG
GTGGTTCTCC
CAAGAAGAAG
AGGAAAGTCT
AACCGGTCAT
CATCACCATC
ACCATTGAGT
TTAAACCCGC
TGATCAGCCT
CGACTGTGCC
TTCTAGTTGC
CAGCCATCTG
TTGTTTGCCC
CTCCCCCGTG
CCTTCCTTGA
CCCTGGAAGG
TGCCACTCCC
ACTGTCCTTT
CCTAATAAAA
TGAGGAAATT
GCATCGCATT
GTCTGAGTAG
GTGTCATTCT
ATTCTGGGGG
GTGGGGTGGG
GCAGGACAGC
AAGGGGGAGG
ATTGGGAAGA
CAATAGCAGG
CATGCTGGGG
ATGCGGTGGG
CTCTATGGCT
TCTGAGGCGG
AAAGAACCAG
CTGGGGCTCG
ATACCGTCGA
CCTCTAGCTA
GAGCTTGGCG
TAATCATGGT
CATAGCTGTT
TCCTGTGTGA
AATTGTTATC
CGCTCACAAT
TCCACACAAC
ATACGAGCCG
GAAGCATAAA
GTGTAAAGCC
TAGGGTGCCT
AATGAGTGAG
CTAACTCACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GAACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GACGTCGACG
GATCGGGAGA
TCGATCTCCC
GATCCCCTAG
GGTCGACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATC