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pET-28b-RfxCas13d-His

产品货号:P48683

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pET-28b-RfxCas13d-His
别称: Plasmid#141322
基因长:
质粒宿主: 大肠杆菌
质粒用途: 蛋白表达
片段类型: ORF,CRISPR
片段物种:
原核抗性: Kan
筛选标记:
荧光标记:
启动子: T7
复制子: pBR322
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CCTGATGCGG

TATTTTCTCC

TTACGCATCT

GTGCGGTATT

TCACACCGCA

ATGGTGCACT

61

CTCAGTACAA

TCTGCTCTGA

TGCCGCATAG

TTAAGCCAGT

ATACACTCCG

CTATCGCTAC

121

GTGACTGGGT

CATGGCTGCG

CCCCGACACC

CGCCAACACC

CGCTGACGCG

CCCTGACGGG

181

CTTGTCTGCT

CCCGGCATCC

GCTTACAGAC

AAGCTGTGAC

CGTCTCCGGG

AGCTGCATGT

241

GTCAGAGGTT

TTCACCGTCA

TCACCGAAAC

GCGCGAGGCA

GCTGCGGTAA

AGCTCATCAG

301

CGTGGTCGTG

AAGCGATTCA

CAGATGTCTG

CCTGTTCATC

CGCGTCCAGC

TCGTTGAGTT

361

TCTCCAGAAG

CGTTAATGTC

TGGCTTCTGA

TAAAGCGGGC

CATGTTAAGG

GCGGTTTTTT

421

CCTGTTTGGT

CACTGATGCC

TCCGTGTAAG

GGGGATTTCT

GTTCATGGGG

GTAATGATAC

481

CGATGAAACG

AGAGAGGATG

CTCACGATAC

GGGTTACTGA

TGATGAACAT

GCCCGGTTAC

541

TGGAACGTTG

TGAGGGTAAA

CAACTGGCGG

TATGGATGCG

GCGGGACCAG

AGAAAAATCA

601

CTCAGGGTCA

ATGCCAGCGC

TTCGTTAATA

CAGATGTAGG

TGTTCCACAG

GGTAGCCAGC

661

AGCATCCTGC

GATGCAGATC

CGGAACATAA

TGGTGCAGGG

CGCTGACTTC

CGCGTTTCCA

721

GACTTTACGA

AACACGGAAA

CCGAAGACCA

TTCATGTTGT

TGCTCAGGTC

GCAGACGTTT

781

TGCAGCAGCA

GTCGCTTCAC

GTTCGCTCGC

GTATCGGTGA

TTCATTCTGC

TAACCAGTAA

841

GGCAACCCCG

CCAGCCTAGC

CGGGTCCTCA

ACGACAGGAG

CACGATCATG

CGCACCCGTG

901

GGGCCGCCAT

GCCGGCGATA

ATGGCCTGCT

TCTCGCCGAA

ACGTTTGGTG

GCGGGACCAG

961

TGACGAAGGC

TTGAGCGAGG

GCGTGCAAGA

TTCCGAATAC

CGCAAGCGAC

AGGCCGATCA

1021

TCGTCGCGCT

CCAGCGAAAG

CGGTCCTCGC

CGAAAATGAC

CCAGAGCGCT

GCCGGCACCT

1081

GTCCTACGAG

TTGCATGATA

AAGAAGACAG

TCATAAGTGC

GGCGACGATA

GTCATGCCCC

1141

GCGCCCACCG

GAAGGAGCTG

ACTGGGTTGA

AGGCTCTCAA

GGGCATCGGT

CGAGATCCCG

1201

GTGCCTAATG

AGTGAGCTAA

CTTACATTAA

TTGCGTTGCG

CTCACTGCCC

GCTTTCCAGT

1261

CGGGAAACCT

GTCGTGCCAG

CTGCATTAAT

GAATCGGCCA

ACGCGCGGGG

AGAGGCGGTT

1321

TGCGTATTGG

GCGCCAGGGT

GGTTTTTCTT

TTCACCAGTG

AGACGGGCAA

CAGCTGATTG

1381

CCCTTCACCG

CCTGGCCCTG

AGAGAGTTGC

AGCAAGCGGT

CCACGCTGGT

TTGCCCCAGC

1441

AGGCGAAAAT

CCTGTTTGAT

GGTGGTTAAC

GGCGGGATAT

AACATGAGCT

GTCTTCGGTA

1501

TCGTCGTATC

CCACTACCGA

GATATCCGCA

CCAACGCGCA

GCCCGGACTC

GGTAATGGCG

1561

CGCATTGCGC

CCAGCGCCAT

CTGATCGTTG

GCAACCAGCA

TCGCAGTGGG

AACGATGCCC

1621

TCATTCAGCA

TTTGCATGGT

TTGTTGAAAA

CCGGACATGG

CACTCCAGTC

GCCTTCCCGT

1681

TCCGCTATCG

GCTGAATTTG

ATTGCGAGTG

AGATATTTAT

GCCAGCCAGC

CAGACGCAGA

1741

CGCGCCGAGA

CAGAACTTAA

TGGGCCCGCT

AACAGCGCGA

TTTGCTGGTG

ACCCAATGCG

1801

ACCAGATGCT

CCACGCCCAG

TCGCGTACCG

TCTTCATGGG

AGAAAATAAT

ACTGTTGATG

1861

GGTGTCTGGT

CAGAGACATC

AAGAAATAAC

GCCGGAACAT

TAGTGCAGGC

AGCTTCCACA

1921

GCAATGGCAT

CCTGGTCATC

CAGCGGATAG

TTAATGATCA

GCCCACTGAC

GCGTTGCGCG

1981

AGAAGATTGT

GCACCGCCGC

TTTACAGGCT

TCGACGCCGC

TTCGTTCTAC

CATCGACACC

2041

ACCACGCTGG

CACCCAGTTG

ATCGGCGCGA

GATTTAATCG

CCGCGACAAT

TTGCGACGGC

2101

GCGTGCAGGG

CCAGACTGGA

GGTGGCAACG

CCAATCAGCA

ACGACTGTTT

GCCCGCCAGT

2161

TGTTGTGCCA

CGCGGTTGGG

AATGTAATTC

AGCTCCGCCA

TCGCCGCTTC

CACTTTTTCC

2221

CGCGTTTTCG

CAGAAACGTG

GCTGGCCTGG

TTCACCACGC

GGGAAACGGT

CTGATAAGAG

2281

ACACCGGCAT

ACTCTGCGAC

ATCGTATAAC

GTTACTGGTT

TCACATTCAC

CACCCTGAAT

2341

TGACTCTCTT

CCGGGCGCTA

TCATGCCATA

CCGCGAAAGG

TTTTGCGCCA

TTCGATGGTG

2401

TCCGGGATCT

CGACGCTCTC

CCTTATGCGA

CTCCTGCATT

AGGAAGCAGC

CCAGTAGTAG

2461

GTTGAGGCCG

TTGAGCACCG

CCGCCGCAAG

GAATGGTGCA

TGCAAGGAGA

TGGCGCCCAA

2521

CAGTCCCCCG

GCCACGGGGC

CTGCCACCAT

ACCCACGCCG

AAACAAGCGC

TCATGAGCCC

2581

GAAGTGGCGA

GCCCGATCTT

CCCCATCGGT

GATGTCGGCG

ATATAGGCGC

CAGCAACCGC

2641

ACCTGTGGCG

CCGGTGATGC

CGGCCACGAT

GCGTCCGGCG

TAGAGGATCG

AGATCTCGAT

2701

CCCGCGAAAT

TAATACGACT

CACTATAGGG

GAATTGTGAG

CGGATAACAA

TTCCCCTCTA

2761

GAAATAATTT

TGTTTAACTT

TAAGAAGGAG

ATATACCATG

GCGAGCGAGG

CCAGCATCGA

2821

AAAAAAAAAG

TCCTTCGCCA

AGGGCATGGG

CGTGAAGTCC

ACACTCGTGT

CCGGCTCCAA

2881

AGTGTACATG

ACAACCTTCG

CCGAAGGCAG

CGACGCCAGG

CTGGAAAAGA

TCGTGGAGGG

2941

CGACAGCATC

AGGAGCGTGA

ATGAGGGCGA

GGCCTTCAGC

GCTGAAATGG

CCGATAAAAA

3001

CGCCGGCTAT

AAGATCGGCA

ACGCCAAATT

CAGCCATCCT

AAGGGCTACG

CCGTGGTGGC

3061

TAACAACCCT

CTGTATACAG

GACCCGTCCA

GCAGGATATG

CTCGGCCTGA

AGGAAACTCT

3121

GGAAAAGAGG

TACTTCGGCG

AGAGCGCTGA

TGGCAATGAC

AATATTTGTA

TCCAGGTGAT

3181

CCATAACATC

CTGGACATTG

AAAAAATCCT

CGCCGAATAC

ATTACCAACG

CCGCCTACGC

3241

CGTCAACAAT

ATCTCCGGCC

TGGATAAGGA

CATTATTGGA

TTCGGCAAGT

TCTCCACAGT

3301

GTATACCTAC

GACGAATTCA

AAGACCCCGA

GCACCATAGG

GCCGCTTTCA

ACAATAACGA

3361

TAAGCTCATC

AACGCCATCA

AGGCCCAGTA

TGACGAGTTC

GACAACTTCC

TCGATAACCC

3421

CAGACTCGGC

TATTTCGGCC

AGGCCTTTTT

CAGCAAGGAG

GGCAGAAATT

ACATCATCAA

3481

TTACGGCAAC

GAATGCTATG

ACATTCTGGC

CCTCCTGAGC

GGACTGAGGC

ACTGGGTGGT

3541

CCATAACAAC

GAAGAAGAGT

CCAGGATCTC

CAGGACCTGG

CTCTACAACC

TCGATAAGAA

3601

CCTCGACAAC

GAATACATCT

CCACCCTCAA

CTACCTCTAC

GACAGGATCA

CCAATGAGCT

3661

GACCAACTCC

TTCTCCAAGA

ACTCCGCCGC

CAACGTGAAC

TATATTGCCG

AAACTCTGGG

3721

AATCAACCCT

GCCGAATTCG

CCGAACAATA

TTTCAGATTC

AGCATTATGA

AAGAGCAGAA

3781

AAACCTCGGA

TTCAATATCA

CCAAGCTCAG

GGAAGTGATG

CTGGACAGGA

AGGATATGTC

3841

CGAGATCAGG

AAAAATCATA

AGGTGTTCGA

CTCCATCAGG

ACCAAGGTCT

ACACCATGAT

3901

GGACTTTGTG

ATTTATAGGT

ATTACATCGA

AGAGGATGCC

AAGGTGGCTG

CCGCCAATAA

3961

GTCCCTCCCC

GATAATGAGA

AGTCCCTGAG

CGAGAAGGAT

ATCTTTGTGA

TTAACCTGAG

4021

GGGCTCCTTC

AACGACGACC

AGAAGGATGC

CCTCTACTAC

GATGAAGCTA

ATAGAATTTG

4081

GAGAAAGCTC

GAAAATATCA

TGCACAACAT

CAAGGAATTT

AGGGGAAACA

AGACAAGAGA

4141

GTATAAGAAG

AAGGACGCCC

CTAGACTGCC

CAGAATCCTG

CCCGCTGGCC

GTGATGTTTC

4201

CGCCTTCAGC

AAACTCATGT

ATGCCCTGAC

CATGTTCCTG

GATGGCAAGG

AGATCAACGA

4261

CCTCCTGACC

ACCCTGATTA

ATAAATTCGA

TAACATCCAG

AGCTTCCTGA

AGGTGATGCC

4321

TCTCATCGGA

GTCAACGCTA

AGTTCGTGGA

GGAATACGCC

TTTTTCAAAG

ACTCCGCCAA

4381

GATCGCCGAT

GAGCTGAGGC

TGATCAAGTC

CTTCGCTAGA

ATGGGAGAAC

CTATTGCCGA

4441

TGCCAGGAGG

GCCATGTATA

TCGACGCCAT

CCGTATTTTA

GGAACCAACC

TGTCCTATGA

4501

TGAGCTCAAG

GCCCTCGCCG

ACACCTTTTC

CCTGGACGAG

AACGGAAACA

AGCTCAAGAA

4561

AGGCAAGCAC

GGCATGAGAA

ATTTCATTAT

TAATAACGTG

ATCAGCAATA

AAAGGTTCCA

4621

CTACCTGATC

AGATACGGTG

ATCCTGCCCA

CCTCCATGAG

ATCGCCAAAA

ACGAGGCCGT

4681

GGTGAAGTTC

GTGCTCGGCA

GGATCGCTGA

CATCCAGAAA

AAACAGGGCC

AGAACGGCAA

4741

GAACCAGATC

GACAGGTACT

ACGAAACTTG

TATCGGAAAG

GATAAGGGCA

AGAGCGTGAG

4801

CGAAAAGGTG

GACGCTCTCA

CAAAGATCAT

CACCGGAATG

AACTACGACC

AATTCGACAA

4861

GAAAAGGAGC

GTCATTGAGG

ACACCGGCAG

GGAAAACGCC

GAGAGGGAGA

AGTTTAAAAA

4921

GATCATCAGC

CTGTACCTCA

CCGTGATCTA

CCACATCCTC

AAGAATATTG

TCAATATCAA

4981

CGCCAGGTAC

GTCATCGGAT

TCCATTGCGT

CGAGCGTGAT

GCTCAACTGT

ACAAGGAGAA

5041

AGGCTACGAC

ATCAATCTCA

AGAAACTGGA

AGAGAAGGGA

TTCAGCTCCG

TCACCAAGCT

5101

CTGCGCTGGC

ATTGATGAAA

CTGCCCCCGA

TAAGAGAAAG

GACGTGGAAA

AGGAGATGGC

5161

TGAAAGAGCC

AAGGAGAGCA

TTGACAGCCT

CGAGAGCGCC

AACCCCAAGC

TGTATGCCAA

5221

TTACATCAAA

TACAGCGACG

AGAAGAAAGC

CGAGGAGTTC

ACCAGGCAGA

TTAACAGGGA

5281

GAAGGCCAAA

ACCGCCCTGA

ACGCCTACCT

GAGGAACACC

AAGTGGAATG

TGATCATCAG

5341

GGAGGACCTC

CTGAGAATTG

ACAACAAGAC

ATGTACCCTG

TTCAGAAACA

AGGCCGTCCA

5401

CCTGGAAGTG

GCCAGGTATG

TCCACGCCTA

TATCAACGAC

ATTGCCGAGG

TCAATTCCTA

5461

CTTCCAACTG

TACCATTACA

TCATGCAGAG

AATTATCATG

AATGAGAGGT

ACGAGAAAAG

5521

CAGCGGAAAG

GTGTCCGAGT

ACTTCGACGC

TGTGAATGAC

GAGAAGAAGT

ACAACGATAG

5581

GCTCCTGAAA

CTGCTGTGTG

TGCCTTTCGG

CTACTGTATC

CCCAGGTTTA

AGAACCTGAG

5641

CATCGAGGCC

CTGTTCGATA

GGAACGAGGC

CGCCAAGTTC

GACAAGGAGA

AAAAGAAGGT

5701

GTCCGGCAAT

TCCGGATCCG

GAGCGGCCGC

ACTCGAGCAC

CACCACCACC

ACCACTGAGA

5761

TCCGGCTGCT

AACAAAGCCC

GAAAGGAAGC

TGAGTTGGCT

GCTGCCACCG

CTGAGCAATA

5821

ACTAGCATAA

CCCCTTGGGG

CCTCTAAACG

GGTCTTGAGG

GGTTTTTTGC

TGAAAGGAGG

5881

AACTATATCC

GGATTGGCGA

ATGGGACGCG

CCCTGTAGCG

GCGCATTAAG

CGCGGCGGGT

5941

GTGGTGGTTA

CGCGCAGCGT

GACCGCTACA

CTTGCCAGCG

CCCTAGCGCC

CGCTCCTTTC

6001

GCTTTCTTCC

CTTCCTTTCT

CGCCACGTTC

GCCGGCTTTC

CCCGTCAAGC

TCTAAATCGG

6061

GGGCTCCCTT

TAGGGTTCCG

ATTTAGTGCT

TTACGGCACC

TCGACCCCAA

AAAACTTGAT

6121

TAGGGTGATG

GTTCACGTAG

TGGGCCATCG

CCCTGATAGA

CGGTTTTTCG

CCCTTTGACG

6181

TTGGAGTCCA

CGTTCTTTAA

TAGTGGACTC

TTGTTCCAAA

CTGGAACAAC

ACTCAACCCT

6241

ATCTCGGTCT

ATTCTTTTGA

TTTATAAGGG

ATTTTGCCGA

TTTCGGCCTA

TTGGTTAAAA

6301

AATGAGCTGA

TTTAACAAAA

ATTTAACGCG

AATTTTAACA

AAATATTAAC

GCTTACAATT

6361

TAGGTGGCAC

TTTTCGGGGA

AATGTGCGCG

GAACCCCTAT

TTGTTTATTT

TTCTAAATAC

6421

ATTCAAATAT

GTATCCGCTC

ATGAATTAAT

TCTTAGAAAA

ACTCATCGAG

CATCAAATGA

6481

AACTGCAATT

TATTCATATC

AGGATTATCA

ATACCATATT

TTTGAAAAAG

CCGTTTCTGT

6541

AATGAAGGAG

AAAACTCACC

GAGGCAGTTC

CATAGGATGG

CAAGATCCTG

GTATCGGTCT

6601

GCGATTCCGA

CTCGTCCAAC

ATCAATACAA

CCTATTAATT

TCCCCTCGTC

AAAAATAAGG

6661

TTATCAAGTG

AGAAATCACC

ATGAGTGACG

ACTGAATCCG

GTGAGAATGG

CAAAAGTTTA

6721

TGCATTTCTT

TCCAGACTTG

TTCAACAGGC

CAGCCATTAC

GCTCGTCATC

AAAATCACTC

6781

GCATCAACCA

AACCGTTATT

CATTCGTGAT

TGCGCCTGAG

CGAGACGAAA

TACGCGATCG

6841

CTGTTAAAAG

GACAATTACA

AACAGGAATC

GAATGCAACC

GGCGCAGGAA

CACTGCCAGC

6901

GCATCAACAA

TATTTTCACC

TGAATCAGGA

TATTCTTCTA

ATACCTGGAA

TGCTGTTTTC

6961

CCGGGGATCG

CAGTGGTGAG

TAACCATGCA

TCATCAGGAG

TACGGATAAA

ATGCTTGATG

7021

GTCGGAAGAG

GCATAAATTC

CGTCAGCCAG

TTTAGTCTGA

CCATCTCATC

TGTAACATCA

7081

TTGGCAACGC

TACCTTTGCC

ATGTTTCAGA

AACAACTCTG

GCGCATCGGG

CTTCCCATAC

7141

AATCGATAGA

TTGTCGCACC

TGATTGCCCG

ACATTATCGC

GAGCCCATTT

ATACCCATAT

7201

AAATCAGCAT

CCATGTTGGA

ATTTAATCGC

GGCCTAGAGC

AAGACGTTTC

CCGTTGAATA

7261

TGGCTCATAA

CACCCCTTGT

ATTACTGTTT

ATGTAAGCAG

ACAGTTTTAT

TGTTCATGAC

7321

CAAAATCCCT

TAACGTGAGT

TTTCGTTCCA

CTGAGCGTCA

GACCCCGTAG

AAAAGATCAA

7381

AGGATCTTCT

TGAGATCCTT

TTTTTCTGCG

CGTAATCTGC

TGCTTGCAAA

CAAAAAAACC

7441

ACCGCTACCA

GCGGTGGTTT

GTTTGCCGGA

TCAAGAGCTA

CCAACTCTTT

TTCCGAAGGT

7501

AACTGGCTTC

AGCAGAGCGC

AGATACCAAA

TACTGTCCTT

CTAGTGTAGC

CGTAGTTAGG

7561

CCACCACTTC

AAGAACTCTG

TAGCACCGCC

TACATACCTC

GCTCTGCTAA

TCCTGTTACC

7621

AGTGGCTGCT

GCCAGTGGCG

ATAAGTCGTG

TCTTACCGGG

TTGGACTCAA

GACGATAGTT

7681

ACCGGATAAG

GCGCAGCGGT

CGGGCTGAAC

GGGGGGTTCG

TGCACACAGC

CCAGCTTGGA

7741

GCGAACGACC

TACACCGAAC

TGAGATACCT

ACAGCGTGAG

CTATGAGAAA

GCGCCACGCT

7801

TCCCGAAGGG

AGAAAGGCGG

ACAGGTATCC

GGTAAGCGGC

AGGGTCGGAA

CAGGAGAGCG

7861

CACGAGGGAG

CTTCCAGGGG

GAAACGCCTG

GTATCTTTAT

AGTCCTGTCG

GGTTTCGCCA

7921

CCTCTGACTT

GAGCGTCGAT

TTTTGTGATG

CTCGTCAGGG

GGGCGGAGCC

TATGGAAAAA

7981

CGCCAGCAAC

GCGGCCTTTT

TACGGTTCCT

GGCCTTTTGC

TGGCCTTTTG

CTCACATGTT

8041

CTTTCCTGCG

TTATCCCCTG

ATTCTGTGGA

TAACCGTATT

ACCGCCTTTG

AGTGAGCTGA

8101

TACCGCTCGC

CGCAGCCGAA

CGACCGAGCG

CAGCGAGTCA

GTGAGCGAGG

AAGCGGAAGA

8161

GCG

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