名称: | pSFVCs-lacZ |
---|---|
别称: | Plasmid#92076 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | LacZ |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ATGGCCGCCA
AAGTGCATGT
TGATATTGAG
GCTGACAGCC
CATTCATCAA
GTCTTTGCAG
AAGGCATTTC
CGTCGTTCGA
GGTGGAGTCA
TTGCAGGTCA
CACCAAATGA
CCATGCAAAT
GCCAGAGCAT
TTTCGCACCT
GGCTACCAAA
TTGATCGAGC
AGGAGACTGA
CAAAGACACA
CTCATCTTGG
ATATCGGCAG
TGCGCCTTCC
AGGAGAATGA
TGTCTACGCA
CAAATACCAC
TGCGTATGCC
CTATGCGCAG
CGCAGAAGAC
CCCGAAAGGC
TCGTATGCTA
CGCAAAGAAA
CTGGCAGCGG
CCTCCGGGAA
GGTGCTGGAT
AGAGAGATCG
CAGGAAAAAT
CACCGACCTG
CAGACCGTCA
TGGCTACGCC
AGACGCTGAA
TCTCCTACCT
TTTGCCTGCA
TACAGACGTC
ACGTGTCGTA
CGGCAGCCGA
AGTGGCCGTA
TACCAGGACG
TGTATGCTGT
ACATGCACCA
ACATCGCTGT
ACCATCAGGC
GATGAAAGGT
GTCAGAACGG
CGTATTGGAT
TGGGTTTGAC
ACCACCCCGT
TTATGTTTGA
CGCGCTAGCA
GGCGCGTATC
CAACCTACGC
CACAAACTGG
GCCGACGAGC
AGGTGTTACA
GGCCAGGAAC
ATAGGACTGT
GTGCAGCATC
CTTGACTGAG
GGAAGACTCG
GCAAACTGTC
CATTCTCCGC
AAGAAGCAAT
TGAAACCTTG
CGACACAGTC
ATGTTCTCGG
TAGGATCTAC
ATTGTACACT
GAGAGCAGAA
AGCTACTGAG
GAGCTGGCAC
TTACCCTCCG
TATTCCACCT
GAAAGGTAAA
CAATCCTTTA
CCTGTAGGTG
CGATACCATC
GTATCATGTG
AAGGGTACGT
AGTTAAGAAA
ATCACTATGT
GCCCCGGCCT
GTACGGTAAA
ACGGTAGGGT
ACGCCGTGAC
GTATCACGCG
GAGGGATTCC
TAGTGTGCAA
GACCACAGAC
ACTGTCAAAG
GAGAAAGAGT
CTCATTCCCT
GTATGCACCT
ACGTCCCCTC
AACCATCTGT
GATCAAATGA
CTGGCATACT
AGCGACCGAC
GTCACACCGG
AGGACGCACA
GAAGTTGTTA
GTGGGATTGA
ATCAGAGGAT
AGTTGTGAAC
GGAAGAACAC
AGCGAAACAC
TAACACGATG
AAGAACTATC
TGCTTCCGAT
TGTGGCCGTC
GCATTTAGCA
AGTGGGCGAG
GGAATACAAG
GCAGACCTTG
ATGATGAAAA
ACCTCTGGGT
GTCCGAGAGA
GGTCACTTAC
TTGCTGCTGC
TTGTGGGCAT
TTAAAACGAG
GAAGATGCAC
ACCATGTACA
AGAAACCAGA
CACCCAGACA
ATAGTGAAGG
TGCCTTCAGA
GTTTAACTCG
TTCGTCATCC
CGAGCCTATG
GTCTACAGGC
CTCGCAATCC
CAGTCAGATC
ACGCATTAAG
ATGCTTTTGG
CCAAGAAGAC
CAAGCGAGAG
TTAATACCTG
TTCTCGACGC
GTCGTCAGCC
AGGGATGCTG
AACAAGAGGA
GAAGGAGAGG
TTGGAGGCCG
AGCTGACTAG
AGAAGCCTTA
CCACCCCTCG
TCCCCATCGC
GCCGGCGGAG
ACGGGAGTCG
TCGACGTCGA
CGTTGAAGAA
CTAGAGTATC
ACGCAGGTGC
AGGGGTCGTG
GAAACACCTC
GCAGCGCGTT
GAAAGTCACC
GCACAGCCGA
ACGACGTACT
ACTAGGAAAT
TACGTAGTTC
TGTCCCCGCA
GACCGTGCTC
AAGAGCTCCA
AGTTGGCCCC
CGTGCACCCT
CTAGCAGAGC
AGGTGAAAAT
AATAACACAT
AACGGGAGGG
CCGGCCGTTA
CCAGGTCGAC
GGATATGACG
GCAGGGTCCT
ACTACCATGT
GGATCGGCCA
TTCCGGTCCC
TGAGTTTCAA
GCTTTGAGCG
AGAGCGCCAC
TATGGTGTAC
AACGAAAGGG
AGTTCGTCAA
CAGGAAACTA
TACCATATTG
CCGTTCACGG
ACCGTCGCTG
AACACCGACG
AGGAGAACTA
CGAGAAAGTC
AGAGCTGAAA
GAACTGACGC
CGAGTACGTG
TTCGACGTAG
ATAAAAAATG
CTGCGTCAAG
AGAGAGGAAG
CGTCGGGTTT
GGTGTTGGTG
GGAGAGCTAA
CCAACCCCCC
GTTCCATGAA
TTCGCCTACG
AAGGGCTGAA
GATCAGGCCG
TCGGCACCAT
ATAAGACTAC
AGTAGTAGGA
GTCTTTGGGG
TTCCGGGATC
AGGCAAGTCT
GCTATTATTA
AGAGCCTCGT
GACCAAACAC
GATCTGGTCA
CCAGCGGCAA
GAAGGAGAAC
TGCCAGGAAA
TAGTCAACGA
CGTGAAGAAG
CACCGCGGAC
TGGACATCCA
GGCAAAAACA
GTGGACTCCA
TCCTGCTAAA
CGGGTGTCGT
CGTGCCGTGG
ACATCCTATA
TGTGGACGAG
GCTTTCGCTT
GCCATTCCGG
TACTCTGCTA
GCCCTAATTG
CTCTTGTTAA
ACCTCGGAGC
AAAGTGGTGT
TATGCGGAGA
CCCCAAGCAA
TGCGGATTCT
TCAATATGAT
GCAGCTTAAG
GTGAACTTCA
ACCACAACAT
CTGCACTGAA
GTATGTCATA
AAAGTATATC
CAGACGTTGC
ACGCGTCCAG
TCACGGCCAT
CGTGTCTACG
TTGCACTACG
GAGGCAAGAT
GCGCACGACC
AACCCGTGCA
ACAAACCCAT
AATCATAGAC
ACCACAGGAC
AGACCAAGCC
CAAGCCAGGA
GACATCGTGT
TAACATGCTT
CCGAGGCTGG
GTAAAGCAGC
TGCAGTTGGA
CTACCGTGGA
CACGAAGTCA
TGACAGCAGC
AGCATCTCAG
GGCCTCACCC
GCAAAGGGGT
ATACGCCGTA
AGGCAGAAGG
TGAATGAAAA
TCCCTTGTAT
GCCCCTGCGT
CGGAGCACGT
GAATGTACTG
CTGACGCGCA
CTGAGGATAG
GCTGGTGTGG
AAAACGCTGG
CCGGCGATCC
CTGGATTAAG
GTCCTATCAA
ACATTCCACA
GGGTAACTTT
ACGGCCACAT
TGGAAGAATG
GCAAGAAGAA
CACGACAAAA
TAATGAAGGT
GATTGAAGGA
CCGGCTGCGC
CTGTGGACGC
GTTCCAGAAC
AAAGCGAACG
TGTGTTGGGC
GAAAAGCCTG
GTGCCTGTCC
TGGACACTGC
CGGAATCAGA
CTCACTGCAG
AAGAGTGGAG
CACGATTATT
ACAGCATTTA
AGGAGGACAG
AGCTTACTCT
CCAGTGGTGG
CCTTGAATGA
AATTTGCACC
AAGTACTATG
GAGTTGACCT
GGACAGTGGC
CTGTTTTCTG
CCCCGAAGGT
GTCCCTGTAT
TACGAGAACA
ACCACTGGGA
TAACAGACCT
GGTGGAAGGA
TGTATGGATT
CAATGCCGCA
ACAGCTGCCA
GGCTGGAAGC
TAGACATACC
TTCCTGAAGG
GGCAGTGGCA
TACGGGCAAG
CAGGCAGTTA
TCGCAGAAAG
AAAAATCCAA
CCGCTTTCTG
TGCTGGACAA
TGTAATTCCT
ATCAACCGCA
GGCTGCCGCA
CGCCCTGGTG
GCTGAGTACA
AGACGGTTAA
AGGCAGTAGG
GTTGAGTGGC
TGGTCAATAA
AGTAAGAGGG
TACCACGTCC
TGCTGGTGAG
TGAGTACAAC
CTGGCTTTGC
CTCGACGCAG
GGTCACTTGG
TTGTCACCGC
TGAATGTCAC
AGGCGCCGAT
AGGTGCTACG
ACCTAAGTTT
AGGACTGCCG
GCTGACGCCG
GCAGGTTCGA
CTTGGTCTTT
GTGAACATTC
ACACGGAATT
CAGAATCCAC
CACTACCAGC
AGTGTGTCGA
CCACGCCATG
AAGCTGCAGA
TGCTTGGGGG
AGATGCGCTA
CGACTGCTAA
AACCCGGCGG
CAGCCTCTTG
ATGAGAGCTT
ACGGATACGC
CGATAAAATC
AGCGAAGCCG
TTGTTTCCTC
CTTAAGCAGA
AAGTTCTCGT
CTGCAAGAGT
GTTGCGCCCG
GATTGTGTCA
CCAGCAATAC
AGAAGTGTTC
TTGCTGTTCT
CCAACTTCGA
TAACGGAAAG
AGGCCCTCAA
CCTTACACCA
GATGAATACC
AAGCTGAGTG
CCGTGTATGC
CGGAGAAGCC
ATGCACACGG
CCGGGTGTGC
ACCATCCTAC
AGAGTTAAGA
GAGCAGACAT
AGCCACGTGC
ACAGAAGCGG
CTGTGGTTAA
CGCAGCTAAC
GCCCGTGGAA
CTGTAGGGGA
TGGCGTATGC
AGGGCCGTGG
CGAAGAAATG
GCCGTCAGCC
TTTAAGGGAG
AAGCAACACC
AGTGGGCACA
ATTAAAACAG
TCATGTGCGG
CTCGTACCCC
GTCATCCACG
CTGTAGCGCC
TAATTTCTCT
GCCACGACTG
AAGCGGAAGG
GGACCGCGAA
TTGGCCGCTG
TCTACCGGGC
AGTGGCCGCC
GAAGTAAACA
GACTGTCACT
GAGCAGCGTA
GCCATCCCGC
TGCTGTCCAC
AGGAGTGTTC
AGCGGCGGAA
GAGATAGGCT
GCAGCAATCC
CTCAACCATC
TATTCACAGC
AATGGACGCC
ACGGACGCTG
ACGTGACCAT
CTACTGCAGA
GACAAAAGTT
GGGAGAAGAA
AATCCAGGAA
GCCATAGACA
TGAGGACGGC
TGTGGAGTTG
CTCAATGATG
ACGTGGAGCT
GACCACAGAC
TTGGTGAGAG
TGCACCCGGA
CAGCAGCCTG
GTGGGTCGTA
AGGGCTACAG
TACCACTGAC
GGGTCGCTGT
ACTCGTACTT
TGAAGGTACG
AAATTCAACC
AGGCTGCTAT
TGATATGGCA
GAGATACTGA
CGTTGTGGCC
CAGACTGCAA
GAGGCAAACG
AACAGATATG
CCTATACGCG
CTGGGCGAAA
CAATGGACAA
CATCAGATCC
AAATGTCCGG
TGAACGATTC
CGATTCATCA
ACACCTCCCA
GGACAGTGCC
CTGCCTGTGC
CGCTACGCAA
TGACAGCAGA
ACGGATCGCC
CGCCTTAGGT
CACACCAAGT
TAAAAGCATG
GTGGTTTGCT
CATCTTTTCC
CCTCCCGAAA
TACCATGTAG
ATGGGGTGCA
GAAGGTAAAG
TGCGAGAAGG
TTCTCCTGTT
CGACCCGACG
GTACCTTCAG
TGGTTAGTCC
GCGGAAGTAT
GCCGCATCTA
CGACGGACCA
CTCAGATCGG
TCGTTACGAG
GGTTTGACTT
GGACTGGACC
ACCGACTCGT
CTTCCACTGC
CAGCGATACC
ATGTCGCTAC
CCAGTTTGCA
GTCGTGTGAC
ATCGACTCGA
TCTACGAGCC
AATGGCTCCC
ATAGTAGTGA
CGGCTGACGT
ACACCCTGAA
CCCGCAGGCA
TCGCGGACCT
GGCGGCAGAT
GTGCATCCTG
AACCCGCAGA
CCATGTGGAC
CTCGAGAACC
CGATTCCTCC
ACCGCGCCCG
AAGAGAGCTG
CATACCTTGC
CTCCCGCGCG
GCGGAGCGAC
CGGTGCCGGC
GCCGAGAAAG
CCGACGCCTG
CCCCAAGGAC
TGCGTTTAGG
AACAAGCTGC
CTTTGACGTT
CGGCGACTTT
GACGAGCACG
AGGTCGATGC
GTTGGCCTCC
GGGATTACTT
TCGGAGACTT
CGACGACGTC
CTGCGACTAG
GCCGCGCGGG
TGCATATATT
TTCTCCTCGG
ACACTGGCAG
CGGACATTTA
CAACAAAAAT
CCGTTAGGCA
GCACAATCTC
CAGTGCGCAC
AACTGGATGC
GGTCGAGGAG
GAGAAAATGT
ACCCGCCAAA
ATTGGATACT
GAGAGGGAGA
AGCTGTTGCT
GCTGAAAATG
CAGATGCACC
CATCGGAGGC
TAATAAGAGT
CGATACCAGT
CTCGCAAAGT
GGAGAACATG
AAAGCCACGG
TGGTGGACAG
GCTCACATCG
GGGGCCAGAT
TGTACACGGG
AGCGGACGTA
GGCCGCATAC
CAACATACGC
GGTTCGGTAC
CCCCGCCCCG
TGTACTCCCC
TACCGTGATC
GAAAGATTCT
CAAGCCCCGA
TGTAGCAATC
GCAGCGTGCA
ACGAATACCT
ATCCAGAAAT
TACCCAACAG
TGGCGTCGTA
CCAGATAACA
GATGAATACG
ACGCATACTT
GGACATGGTT
GACGGGTCGG
ATAGTTGCTT
GGACAGAGCG
ACATTCTGCC
CGGCGAAGCT
CCGGTGCTAC
CCGAAACATC
ATGCGTACCA
CCAGCCGACT
GTACGCAGTG
CCGTCCCGTC
ACCCTTTCAG
AACACACTAC
AGAACGTGCT
AGCGGCCGCC
ACCAAGAGAA
ACTGCAACGT
CACGCAAATG
CGAGAACTAC
CCACCATGGA
CTCGGCAGTG
TTCAACGTGG
AGTGCTTCAA
GCGCTATGCC
TGCTCCGGAG
AATATTGGGA
AGAATATGCT
AAACAACCTA
TCCGGATAAC
CACTGAGAAC
ATCACTACCT
ATGTGACCAA
ATTGAAAGGC
CCGAAAGCTG
CTGCCTTGTT
CGCTAAGACC
CACAACTTGG
TTCCGCTGCA
GGAGGTTCCC
ATGGACAGAT
TCACGGTCGA
CATGAAACGA
GATGTCAAAG
TCACTCCAGG
GACGAAACAC
ACAGAGGAAA
GACCCAAAGT
CCAGGTAATT
CAAGCAGCGG
AGCCATTGGC
GACCGCTTAC
CTGTGCGGCA
TCCACAGGGA
ATTAGTAAGG
AGACTAAATG
CTGTGTTACG
CCCTAACGTG
CACACATTGT
TTGATATGTC
GGCCGAAGAC
TTTGACGCGA
TCATCGCCTC
TCACTTCCAC
CCAGGAGACC
CGGTTCTAGA
GACGGACATT
GCATCATTCG
ACAAAAGCCA
GGACGACTCC
TTGGCTCTTA
CAGGTTTAAT
GATCCTCGAA
GATCTAGGGG
TGGATCAGTA
CCTGCTGGAC
TTGATCGAGG
CAGCCTTTGG
GGAAATATCC
AGCTGTCACC
TACCAACTGG
CACGCGCTTC
AAGTTCGGAG
CTATGATGAA
ATCGGGCATG
TTTCTGACTT
TGTTTATTAA
CACTGTTTTG
AACATCACCA
TAGCAAGCAG
GGTACTGGAG
CAGAGACTCA
CTGACTCCGC
CTGTGCGGCC
TTCATCGGCG
ACGACAACAT
CGTTCACGGA
GTGATCTCCG
ACAAGCTGAT
GGCGGAGAGG
TGCGCGTCGT
GGGTCAACAT
GGAGGTGAAG
ATCATTGACG
CTGTCATGGG
CGAAAAACCC
CCATATTTTT
GTGGGGGATT
CATAGTTTTT
GACAGCGTCA
CACAGACCGC
CTGCCGTGTT
TCAGACCCAC
TTAAGCGCCT
GTTCAAGTTG
GGTAAGCCGC
TAACAGCTGA
AGACAAGCAG
GACGAAGACA
GGCGACGAGC
ACTGAGTGAC
GAGGTTAGCA
AGTGGTTCCG
GACAGGCTTG
GGGGCCGAAC
TGGAGGTGGC
ACTAACATCT
AGGTATGAGG
TAGAGGGCTG
CAAAAGTATC
CTCATAGCCA
TGGCCACCTT
GGCGAGGGAC
ATTAAGGCGT
TTAAGAAATT
GAGAGGACCT
GTTATACACC
TCTACGGCGG
TCCTAGATTG
GTGCGTTAAT
ACACAGAATT
CTGATTATAG
CGCACTATTA
TAGCACCATG
AATTACATCC
CTACGCAAAC
GTTTTACGGC
CGCCGGTGGC
GCCCGCGCCC
GGCGGCCCGT
CCCTGGCCGT
TGCAGGCCAC
TCCGGTGGCT
CCCGTCGTCC
CCGACTTCCA
GGCCCAGCAG
ATGCAGCAAC
TCATCAGCGC
CGTAAATGCG
CTGACAATGA
GACAGAACGC
AATTGCTCCT
GCTAGGCCTC
CCAAACCAAA
GAAGAAGAAG
ACAACCAAAC
CAAAGCCGAA
AACGCAGCCC
AAGAAGATCA
ACGGAAAAAC
GCAGCAGCAA
AAGAAGAAAG
ACAAGCAAGC
CGACAAGAAG
AAGAAGAAAC
CCGGAAAAAG
AGAAAGAATG
TGCATGAAGA
TTGAAAATGA
CTGTATCTTC
GAAGTCAAAC
ACGAAGGAAA
GGTCACTGGG
TACGCCTGCC
TGGTGGGCGA
CAAAGTCATG
AAACCTGCCC
ACGTGAAAGG
AGTCATCGAC
AACGCGGACC
TGGCAAAGCT
AGCTTTCAAG
AAATCGAGCA
AGTATGACCT
TGAGTGTGCC
CAGATACCAG
TTCACATGAG
GTCGGATGCC
TCAAAGTACA
CGCATGAGAA
GCCCGAGGGA
CACTATAACT
GGCACCACGG
GGCTGTTCAG
TACAGCGGAG
GTAGGTTCAC
TATACCGACA
GGAGCGGGCA
AACCGGGAGA
CAGTGGCAGG
CCCATATTCG
ATAACAAGGG
GAGGGTAGTC
GCAATCGTCC
TGGGTGGGGC
CAACGAGGGC
TCACGCACAG
CACTGTCGGT
GGTCACCTGG
AACAAAGATA
TGGTGACTAG
AGTGACCCCC
GAGGGGTCCG
AAGAGTGGGA
TCCCGTCGTT
TTACAACGTC
GTGACTGGGA
AAACCCTGGC
GTTACCCAAC
TTAATCGCCT
TGCAGCACAT
CCCCCTTTCG
CCAGCTGGCG
TAATAGCGAA
GAGGCCCGCA
CCGATCGCCC
TTCCCAACAG
TTGCGCAGCC
TGAATGGCGA
ATGGCGCTTT
GCCTGGTTTC
CGGCACCAGA
AGCGGTGCCG
GAAAGCTGGC
TGGAGTGCGA
TCTTCCTGAG
GCCGATACTG
TCGTCGTCCC
CTCAAACTGG
CAGATGCACG
GTTACGATGC
GCCCATCTAC
ACCAACGTGA
CCTATCCCAT
TACGGTCAAT
CCGCCGTTTG
TTCCCACGGA
GAATCCGACG
GGTTGTTACT
CGCTCACATT
TAATGTTGAT
GAAAGCTGGC
TACAGGAAGG
CCAGACGCGA
ATTATTTTTG
ATGGCGTTAA
CTCGGCGTTT
CATCTGTGGT
GCAACGGGCG
CTGGGTCGGT
TACGGCCAGG
ACAGTCGTTT
GCCGTCTGAA
TTTGACCTGA
GCGCATTTTT
ACGCGCCGGA
GAAAACCGCC
TCGCGGTGAT
GGTGCTGCGC
TGGAGTGACG
GCAGTTATCT
GGAAGATCAG
GATATGTGGC
GGATGAGCGG
CATTTTCCGT
GACGTCTCGT
TGCTGCATAA
ACCGACTACA
CAAATCAGCG
ATTTCCATGT
TGCCACTCGC
TTTAATGATG
ATTTCAGCCG
CGCTGTACTG
GAGGCTGAAG
TTCAGATGTG
CGGCGAGTTG
CGTGACTACC
TACGGGTAAC
AGTTTCTTTA
TGGCAGGGTG
AAACGCAGGT
CGCCAGCGGC
ACCGCGCCTT
TCGGCGGTGA
AATTATCGAT
GAGCGTGGTG
GTTATGCCGA
TCGCGTCACA
CTACGTCTGA
ACGTCGAAAA
CCCGAAACTG
TGGAGCGCCG
AAATCCCGAA
TCTCTATCGT
GCGGTGGTTG
AACTGCACAC
CGCCGACGGC
ACGCTGATTG
AAGCAGAAGC
CTGCGATGTC
GGTTTCCGCG
AGGTGCGGAT
TGAAAATGGT
CTGCTGCTGC
TGAACGGCAA
GCCGTTGCTG
ATTCGAGGCG
TTAACCGTCA
CGAGCATCAT
CCTCTGCATG
GTCAGGTCAT
GGATGAGCAG
ACGATGGTGC
AGGATATCCT
GCTGATGAAG
CAGAACAACT
TTAACGCCGT
GCGCTGTTCG
CATTATCCGA
ACCATCCGCT
GTGGTACACG
CTGTGCGACC
GCTACGGCCT
GTATGTGGTG
GATGAAGCCA
ATATTGAAAC
CCACGGCATG
GTGCCAATGA
ATCGTCTGAC
CGATGATCCG
CGCTGGCTAC
CGGCGATGAG
CGAACGCGTA
ACGCGAATGG
TGCAGCGCGA
TCGTAATCAC
CCGAGTGTGA
TCATCTGGTC
GCTGGGGAAT
GAATCAGGCC
ACGGCGCTAA
TCACGACGCG
CTGTATCGCT
GGATCAAATC
TGTCGATCCT
TCCCGCCCGG
TGCAGTATGA
AGGCGGCGGA
GCCGACACCA
CGGCCACCGA
TATTATTTGC
CCGATGTACG
CGCGCGTGGA
TGAAGACCAG
CCCTTCCCGG
CTGTGCCGAA
ATGGTCCATC
AAAAAATGGC
TTTCGCTACC
TGGAGAGACG
CGCCCGCTGA
TCCTTTGCGA
ATACGCCCAC
GCGATGGGTA
ACAGTCTTGG
CGGTTTCGCT
AAATACTGGC
AGGCGTTTCG
TCAGTATCCC
CGTTTACAGG
GCGGCTTCGT
CTGGGACTGG
GTGGATCAGT
CGCTGATTAA
ATATGATGAA
AACGGCAACC
CGTGGTCGGC
TTACGGCGGT
GATTTTGGCG
ATACGCCGAA
CGATCGCCAG
TTCTGTATGA
ACGGTCTGGT
CTTTGCCGAC
CGCACGCCGC
ATCCAGCGCT
GACGGAAGCA
AAACACCAGC
AGCAGTTTTT
CCAGTTCCGT
TTATCCGGGC
AAACCATCGA
AGTGACCAGC
GAATACCTGT
TCCGTCATAG
CGATAACGAG
CTCCTGCACT
GGATGGTGGC
GCTGGATGGT
AAGCCGCTGG
CAAGCGGTGA
AGTGCCTCTG
GATGTCGCTC
CACAAGGTAA
ACAGTTGATT
GAACTGCCTG
AACTACCGCA
GCCGGAGAGC
GCCGGGCAAC
TCTGGCTCAC
AGTACGCGTA
GTGCAACCGA
ACGCGACCGC
ATGGTCAGAA
GCCGGGCACA
TCAGCGCCTG
GCAGCAGTGG
CGTCTGGCGG
AAAACCTCAG
TGTGACGCTC
CCCGCCGCGT
CCCACGCCAT
CCCGCATCTG
ACCACCAGCG
AAATGGATTT
TTGCATCGAG
CTGGGTAATA
AGCGTTGGCA
ATTTAACCGC
CAGTCAGGCT
TTCTTTCACA
GATGTGGATT
GGCGATAAAA
AACAACTGCT
GACGCCGCTG
CGCGATCAGT
TCACCCGTGC
ACCGCTGGAT
AACGACATTG
GCGTAAGTGA
AGCGACCCGC
ATTGACCCTA
ACGCCTGGGT
CGAACGCTGG
AAGGCGGCGG
GCCATTACCA
GGCCGAAGCA
GCGTTGTTGC
AGTGCACGGC
AGATACACTT
GCTGATGCGG
TGCTGATTAC
GACCGCTCAC
GCGTGGCAGC
ATCAGGGGAA
AACCTTATTT
ATCAGCCGGA
AAACCTACCG
GATTGATGGT
AGTGGTCAAA
TGGCGATTAC
CGTTGATGTT
GAAGTGGCGA
GCGATACACC
GCATCCGGCG
CGGATTGGCC
TGAACTGCCA
GCTGGCGCAG
GTAGCAGAGC
GGGTAAACTG
GCTCGGATTA
GGGCCGCAAG
AAAACTATCC
CGACCGCCTT
ACTGCCGCCT
GTTTTGACCG
CTGGGATCTG
CCATTGTCAG
ACATGTATAC
CCCGTACGTC
TTCCCGAGCG
AAAACGGTCT
GCGCTGCGGG
ACGCGCGAAT
TGAATTATGG
CCCACACCAG
TGGCGCGGCG
ACTTCCAGTT
CAACATCAGC
CGCTACAGTC
AACAGCAACT
GATGGAAACC
AGCCATCGCC
ATCTGCTGCA
CGCGGAAGAA
GGCACATGGC
TGAATATCGA
CGGTTTCCAT
ATGGGGATTG
GTGGCGACGA
CTCCTGGAGC
CCGTCAGTAT
CGGCGGAATT
CCAGCTGAGC
GCCGGTCGCT
ACCATTACCA
GTTGGTCTGG
TGTCAAAAAT
AATAATAACC
GGGCAGGGGG
GATCCCGGGT
AATTAATTGA
ATTACATCCC
TACGCAAACG
TTTTACGGCC
GCCGGTGGCG
CCCGCGCCCG
GCGGCCCGTC
CCTGGCCGTT
GCAGGCCACT
CCGGTGGCTC
CCGTCGTCCC
CGACTTCCAG
GCCCAGCAGA
TGCAGCAACT
CATCAGCGCC
GTAAATGCGC
TGACAATGAG
ACAGAACGCA
ATTGCTCCTG
CTAGGCCTCC
CAAACCAAAG
AAGAAGAAGA
CAACCAAACC
AAAGCCGAAA
ACGCAGCCCA
AGAAGATCAA
CGGAAAAACG
CAGCAGCAAA
AGAAGAAAGA
CAAGCAAGCC
GACAAGAAGA
AGAAGAAACC
CGGAAAAAGA
GAAAGAATGT
GCATGAAGAT
TGAAAATGAC
TGTATCTATG
CGGCTAGCCA
CAGTAACGTA
GTGTTTCCAG
ACATGTCGGG
CACCGCACTA
TCATGGGTGC
AGAAAATCTC
GGGTGGTCTG
GGGGCCTTCG
CAATCGGCGC
TATCCTGGTG
CTGGTTGTGG
TCACTTGCAT
TGGGCTCCGC
AGATAAGTTA
GGGTAGGCAA
TGGCATTGAT
ATAGCAAGAA
AATTGAAAAC
AGAAAAAGTT
AGGGTAAGCA
ATGGCATATA
ACCATAACTG
TATAACTTGT
AACAAAGCGC
AACAAGACCT
GCGCAATTGG
CCCCGTGGTC
CGCCTCACGG
AAACTCGGGG
CAACTCATAT
TGACACATTA
ATTGGCAATA
ATTGGAAGCT
TACATAAGCT
TAATTCGACG
AATAATTGGA
TTTTTATTTT
ATTTTGCAAT
TGGTTTTTAA
TATTTCCAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAACTA
GTCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGA
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGTCTAAGAA
ACCATTATTA
TCATGACATT
AACCTATAAA
AATAGGCGTA
TCACGAGGCC
CTTTCGTCTC
GCGCGTTTCG
GTGATGACGG
TGAAAACCTC
TGACACATGC
AGCTCCCGGA
GACGGTCACA
GCTTGTCTGT
AAGCGGATGC
CGGGAGCAGA
CAAGCCCGTC
AGGGCGCGTC
AGCGGGTGTT
GGCGGGTGTC
GGGGCTGGCT
TAACTATGCG
GCATCAGAGC
AGATTGTACT
GAGAGTGCAC
CATTCGACGC
TCTCCCTTAT
GCGACTCCTG
CATTAGGAAG
CAGCCCAGTA
GTAGGTTGAG
GCCGTTGAGC
ACCGCCGCCG
CAAGGAATGG
TGCATGCAAG
GAGATGGCGC
CCAACAGTCC
CCCGGCCACG
GGGCCTGCCA
CCATACCCAC
GCCGAAACAA
GCGCTCATGA
GCCCGAAGTG
GCGAGCCCGA
TCTTCCCCAT
CGGTGATGTC
GGCGATATAG
GCGCCAGCAA
CCGCACCTGT
GGCGCCGGTG
ATGCCGGCCA
CGATGCGTCC
GGCGTAGAGG
ATCTGGCTAG
CGATGACCCT
GCTGATTGGT
TCGCTGACCA
TTTCCGGGTG
CGGGACGGCG
TTACCAGAAA
CTCAGAAGGT
TCGTCCAACC
AAACCGACTC
TGACGGCAGT
TTACGAGAGA
GATGATAGGG
TCTGCTTCAG
TAAGCCAGAT
GCTACACAAT
TAGGCTTGTA
CATATTGTCG
TTAGAACGCG
GCTACAATTA
ATACATAACC
TTATGTATCA
TACACATACG
ATTTAGGTGA
CACTATAGAT
GGCGGATGTG
TGACATACAC
GACGCCAAAA
GATTTTGTTC
CAGCTCCTGC
CACCTCCGCT
ACGCGAGAGA
TTAACCACCC
ACG