名称: | pX333 |
---|---|
别称: | Plasmid#64073 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 多框空载 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GGCCTTTTGC
TCACATGCGC
CATTCTCGAG
CATGCGCCAT
TCTCGAGCAT
GCTCGAGAAT
GGCGCATGTG
AGGGCCTATT
TCCCATGATT
CCTTCATATT
TGCATATACG
ATACAAGGCT
GTTAGAGAGA
TAATTGGAAT
TAATTTGACT
GTAAACACAA
AGATATTAGT
ACAAAATACG
TGACGTAGAA
AGTAATAATT
TCTTGGGTAG
TTTGCAGTTT
TAAAATTATG
TTTTAAAATG
GACTATCATA
TGCTTACCGT
AACTTGAAAG
TATTTCGATT
TCTTGGCTTT
ATATATCTTG
TGGAAAGGAC
GAAACACCGG
GTCTTCGAGA
AGACCTGTTT
TAGAGCTAGA
AATAGCAAGT
TAAAATAAGG
CTAGTCCGTT
ATCAACTTGA
AAAAGTGGCA
CCGAGTCGGT
GCTTTTTTGT
TTTAGAGCTA
GAAATAGCAA
GTTAAAATAA
GGCTAGTCCG
TTTTTAGCGC
GTGCGCCAAT
TCTGCAGACA
AATGGCTCTA
GAGAGGGCCT
ATTTCCCATG
ATTCCTTCAT
ATTTGCATAT
ACGATACAAG
GCTGTTAGAG
AGATAATTGG
AATTAATTTG
ACTGTAAACA
CAAAGATATT
AGTACAAAAT
ACGTGACGTA
GAAAGTAATA
ATTTCTTGGG
TAGTTTGCAG
TTTTAAAATT
ATGTTTTAAA
ATGGACTATC
ATATGCTTAC
CGTAACTTGA
AAGTATTTCG
ATTTCTTGGC
TTTATATATC
TTGTGGAAAG
GACGAAACAC
CGGAGACCTA
TTCGCCTTAA
GGTCTCGGTT
TTAGAGCTAG
AAATAGCAAG
TTAAAATAAG
GCTAGTCCGT
TATCAACTTG
AAAAAGTGGC
ACCGAGTCGG
TGCTTTTTTG
TTTTAGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTTTTAGCG
CGTGCGCCAA
TTCTGCAGAC
AAATGGGGTA
CCCGTTACAT
AACTTACGGT
AAATGGCCCG
CCTGGCTGAC
CGCCCAACGA
CCCCCGCCCA
TTGACGTCAA
TAGTAACGCC
AATAGGGACT
TTCCATTGAC
GTCAATGGGT
GGAGTATTTA
CGGTAAACTG
CCCACTTGGC
AGTACATCAA
GTGTATCATA
TGCCAAGTAC
GCCCCCTATT
GACGTCAATG
ACGGTAAATG
GCCCGCCTGG
CATTGTGCCC
AGTACATGAC
CTTATGGGAC
TTTCCTACTT
GGCAGTACAT
CTACGTATTA
GTCATCGCTA
TTACCATGGT
CGAGGTGAGC
CCCACGTTCT
GCTTCACTCT
CCCCATCTCC
CCCCCCTCCC
CACCCCCAAT
TTTGTATTTA
TTTATTTTTT
AATTATTTTG
TGCAGCGATG
GGGGCGGGGG
GGGGGGGGGG
GCGCGCGCCA
GGCGGGGCGG
GGCGGGGCGA
GGGGCGGGGC
GGGGCGAGGC
GGAGAGGTGC
GGCGGCAGCC
AATCAGAGCG
GCGCGCTCCG
AAAGTTTCCT
TTTATGGCGA
GGCGGCGGCG
GCGGCGGCCC
TATAAAAAGC
GAAGCGCGCG
GCGGGCGGGA
GTCGCTGCGC
GCTGCCTTCG
CCCCGTGCCC
CGCTCCGCCG
CCGCCTCGCG
CCGCCCGCCC
CGGCTCTGAC
TGACCGCGTT
ACTCCCACAG
GTGAGCGGGC
GGGACGGCCC
TTCTCCTCCG
GGCTGTAATT
AGCTGAGCAA
GAGGTAAGGG
TTTAAGGGAT
GGTTGGTTGG
TGGGGTATTA
ATGTTTAATT
ACCTGGAGCA
CCTGCCTGAA
ATCACTTTTT
TTCAGGTTGG
ACCGGTGCCA
CCATGGACTA
TAAGGACCAC
GACGGAGACT
ACAAGGATCA
TGATATTGAT
TACAAAGACG
ATGACGATAA
GATGGCCCCA
AAGAAGAAGC
GGAAGGTCGG
TATCCACGGA
GTCCCAGCAG
CCGACAAGAA
GTACAGCATC
GGCCTGGACA
TCGGCACCAA
CTCTGTGGGC
TGGGCCGTGA
TCACCGACGA
GTACAAGGTG
CCCAGCAAGA
AATTCAAGGT
GCTGGGCAAC
ACCGACCGGC
ACAGCATCAA
GAAGAACCTG
ATCGGAGCCC
TGCTGTTCGA
CAGCGGCGAA
ACAGCCGAGG
CCACCCGGCT
GAAGAGAACC
GCCAGAAGAA
GATACACCAG
ACGGAAGAAC
CGGATCTGCT
ATCTGCAAGA
GATCTTCAGC
AACGAGATGG
CCAAGGTGGA
CGACAGCTTC
TTCCACAGAC
TGGAAGAGTC
CTTCCTGGTG
GAAGAGGATA
AGAAGCACGA
GCGGCACCCC
ATCTTCGGCA
ACATCGTGGA
CGAGGTGGCC
TACCACGAGA
AGTACCCCAC
CATCTACCAC
CTGAGAAAGA
AACTGGTGGA
CAGCACCGAC
AAGGCCGACC
TGCGGCTGAT
CTATCTGGCC
CTGGCCCACA
TGATCAAGTT
CCGGGGCCAC
TTCCTGATCG
AGGGCGACCT
GAACCCCGAC
AACAGCGACG
TGGACAAGCT
GTTCATCCAG
CTGGTGCAGA
CCTACAACCA
GCTGTTCGAG
GAAAACCCCA
TCAACGCCAG
CGGCGTGGAC
GCCAAGGCCA
TCCTGTCTGC
CAGACTGAGC
AAGAGCAGAC
GGCTGGAAAA
TCTGATCGCC
CAGCTGCCCG
GCGAGAAGAA
GAATGGCCTG
TTCGGAAACC
TGATTGCCCT
GAGCCTGGGC
CTGACCCCCA
ACTTCAAGAG
CAACTTCGAC
CTGGCCGAGG
ATGCCAAACT
GCAGCTGAGC
AAGGACACCT
ACGACGACGA
CCTGGACAAC
CTGCTGGCCC
AGATCGGCGA
CCAGTACGCC
GACCTGTTTC
TGGCCGCCAA
GAACCTGTCC
GACGCCATCC
TGCTGAGCGA
CATCCTGAGA
GTGAACACCG
AGATCACCAA
GGCCCCCCTG
AGCGCCTCTA
TGATCAAGAG
ATACGACGAG
CACCACCAGG
ACCTGACCCT
GCTGAAAGCT
CTCGTGCGGC
AGCAGCTGCC
TGAGAAGTAC
AAAGAGATTT
TCTTCGACCA
GAGCAAGAAC
GGCTACGCCG
GCTACATTGA
CGGCGGAGCC
AGCCAGGAAG
AGTTCTACAA
GTTCATCAAG
CCCATCCTGG
AAAAGATGGA
CGGCACCGAG
GAACTGCTCG
TGAAGCTGAA
CAGAGAGGAC
CTGCTGCGGA
AGCAGCGGAC
CTTCGACAAC
GGCAGCATCC
CCCACCAGAT
CCACCTGGGA
GAGCTGCACG
CCATTCTGCG
GCGGCAGGAA
GATTTTTACC
CATTCCTGAA
GGACAACCGG
GAAAAGATCG
AGAAGATCCT
GACCTTCCGC
ATCCCCTACT
ACGTGGGCCC
TCTGGCCAGG
GGAAACAGCA
GATTCGCCTG
GATGACCAGA
AAGAGCGAGG
AAACCATCAC
CCCCTGGAAC
TTCGAGGAAG
TGGTGGACAA
GGGCGCTTCC
GCCCAGAGCT
TCATCGAGCG
GATGACCAAC
TTCGATAAGA
ACCTGCCCAA
CGAGAAGGTG
CTGCCCAAGC
ACAGCCTGCT
GTACGAGTAC
TTCACCGTGT
ATAACGAGCT
GACCAAAGTG
AAATACGTGA
CCGAGGGAAT
GAGAAAGCCC
GCCTTCCTGA
GCGGCGAGCA
GAAAAAGGCC
ATCGTGGACC
TGCTGTTCAA
GACCAACCGG
AAAGTGACCG
TGAAGCAGCT
GAAAGAGGAC
TACTTCAAGA
AAATCGAGTG
CTTCGACTCC
GTGGAAATCT
CCGGCGTGGA
AGATCGGTTC
AACGCCTCCC
TGGGCACATA
CCACGATCTG
CTGAAAATTA
TCAAGGACAA
GGACTTCCTG
GACAATGAGG
AAAACGAGGA
CATTCTGGAA
GATATCGTGC
TGACCCTGAC
ACTGTTTGAG
GACAGAGAGA
TGATCGAGGA
ACGGCTGAAA
ACCTATGCCC
ACCTGTTCGA
CGACAAAGTG
ATGAAGCAGC
TGAAGCGGCG
GAGATACACC
GGCTGGGGCA
GGCTGAGCCG
GAAGCTGATC
AACGGCATCC
GGGACAAGCA
GTCCGGCAAG
ACAATCCTGG
ATTTCCTGAA
GTCCGACGGC
TTCGCCAACA
GAAACTTCAT
GCAGCTGATC
CACGACGACA
GCCTGACCTT
TAAAGAGGAC
ATCCAGAAAG
CCCAGGTGTC
CGGCCAGGGC
GATAGCCTGC
ACGAGCACAT
TGCCAATCTG
GCCGGCAGCC
CCGCCATTAA
GAAGGGCATC
CTGCAGACAG
TGAAGGTGGT
GGACGAGCTC
GTGAAAGTGA
TGGGCCGGCA
CAAGCCCGAG
AACATCGTGA
TCGAAATGGC
CAGAGAGAAC
CAGACCACCC
AGAAGGGACA
GAAGAACAGC
CGCGAGAGAA
TGAAGCGGAT
CGAAGAGGGC
ATCAAAGAGC
TGGGCAGCCA
GATCCTGAAA
GAACACCCCG
TGGAAAACAC
CCAGCTGCAG
AACGAGAAGC
TGTACCTGTA
CTACCTGCAG
AATGGGCGGG
ATATGTACGT
GGACCAGGAA
CTGGACATCA
ACCGGCTGTC
CGACTACGAT
GTGGACCATA
TCGTGCCTCA
GAGCTTTCTG
AAGGACGACT
CCATCGACAA
CAAGGTGCTG
ACCAGAAGCG
ACAAGAACCG
GGGCAAGAGC
GACAACGTGC
CCTCCGAAGA
GGTCGTGAAG
AAGATGAAGA
ACTACTGGCG
GCAGCTGCTG
AACGCCAAGC
TGATTACCCA
GAGAAAGTTC
GACAATCTGA
CCAAGGCCGA
GAGAGGCGGC
CTGAGCGAAC
TGGATAAGGC
CGGCTTCATC
AAGAGACAGC
TGGTGGAAAC
CCGGCAGATC
ACAAAGCACG
TGGCACAGAT
CCTGGACTCC
CGGATGAACA
CTAAGTACGA
CGAGAATGAC
AAGCTGATCC
GGGAAGTGAA
AGTGATCACC
CTGAAGTCCA
AGCTGGTGTC
CGATTTCCGG
AAGGATTTCC
AGTTTTACAA
AGTGCGCGAG
ATCAACAACT
ACCACCACGC
CCACGACGCC
TACCTGAACG
CCGTCGTGGG
AACCGCCCTG
ATCAAAAAGT
ACCCTAAGCT
GGAAAGCGAG
TTCGTGTACG
GCGACTACAA
GGTGTACGAC
GTGCGGAAGA
TGATCGCCAA
GAGCGAGCAG
GAAATCGGCA
AGGCTACCGC
CAAGTACTTC
TTCTACAGCA
ACATCATGAA
CTTTTTCAAG
ACCGAGATTA
CCCTGGCCAA
CGGCGAGATC
CGGAAGCGGC
CTCTGATCGA
GACAAACGGC
GAAACCGGGG
AGATCGTGTG
GGATAAGGGC
CGGGATTTTG
CCACCGTGCG
GAAAGTGCTG
AGCATGCCCC
AAGTGAATAT
CGTGAAAAAG
ACCGAGGTGC
AGACAGGCGG
CTTCAGCAAA
GAGTCTATCC
TGCCCAAGAG
GAACAGCGAT
AAGCTGATCG
CCAGAAAGAA
GGACTGGGAC
CCTAAGAAGT
ACGGCGGCTT
CGACAGCCCC
ACCGTGGCCT
ATTCTGTGCT
GGTGGTGGCC
AAAGTGGAAA
AGGGCAAGTC
CAAGAAACTG
AAGAGTGTGA
AAGAGCTGCT
GGGGATCACC
ATCATGGAAA
GAAGCAGCTT
CGAGAAGAAT
CCCATCGACT
TTCTGGAAGC
CAAGGGCTAC
AAAGAAGTGA
AAAAGGACCT
GATCATCAAG
CTGCCTAAGT
ACTCCCTGTT
CGAGCTGGAA
AACGGCCGGA
AGAGAATGCT
GGCCTCTGCC
GGCGAACTGC
AGAAGGGAAA
CGAACTGGCC
CTGCCCTCCA
AATATGTGAA
CTTCCTGTAC
CTGGCCAGCC
ACTATGAGAA
GCTGAAGGGC
TCCCCCGAGG
ATAATGAGCA
GAAACAGCTG
TTTGTGGAAC
AGCACAAGCA
CTACCTGGAC
GAGATCATCG
AGCAGATCAG
CGAGTTCTCC
AAGAGAGTGA
TCCTGGCCGA
CGCTAATCTG
GACAAAGTGC
TGTCCGCCTA
CAACAAGCAC
CGGGATAAGC
CCATCAGAGA
GCAGGCCGAG
AATATCATCC
ACCTGTTTAC
CCTGACCAAT
CTGGGAGCCC
CTGCCGCCTT
CAAGTACTTT
GACACCACCA
TCGACCGGAA
GAGGTACACC
AGCACCAAAG
AGGTGCTGGA
CGCCACCCTG
ATCCACCAGA
GCATCACCGG
CCTGTACGAG
ACACGGATCG
ACCTGTCTCA
GCTGGGAGGC
GACAAAAGGC
CGGCGGCCAC
GAAAAAGGCC
GGCCAGGCAA
AAAAGAAAAA
GTAAGAATTC
CTAGAGCTCG
CTGATCAGCC
TCGACTGTGC
CTTCTAGTTG
CCAGCCATCT
GTTGTTTGCC
CCTCCCCCGT
GCCTTCCTTG
ACCCTGGAAG
GTGCCACTCC
CACTGTCCTT
TCCTAATAAA
ATGAGGAAAT
TGCATCGCAT
TGTCTGAGTA
GGTGTCATTC
TATTCTGGGG
GGTGGGGTGG
GGCAGGACAG
CAAGGGGGAG
GATTGGGAAG
AGAATAGCAG
GCATGCTGGG
GAGCGGCCGC
AGGAACCCCT
AGTGATGGAG
TTGGCCACTC
CCTCTCTGCG
CGCTCGCTCG
CTCACTGAGG
CCGGGCGACC
AAAGGTCGCC
CGACGCCCGG
GCTTTGCCCG
GGCGGCCTCA
GTGAGCGAGC
GAGCGCGCAG
CTGCCTGCAG
GGGCGCCTGA
TGCGGTATTT
TCTCCTTACG
CATCTGTGCG
GTATTTCACA
CCGCATACGT
CAAAGCAACC
ATAGTACGCG
CCCTGTAGCG
GCGCATTAAG
CGCGGCGGGT
GTGGTGGTTA
CGCGCAGCGT
GACCGCTACA
CTTGCCAGCG
CCTTAGCGCC
CGCTCCTTTC
GCTTTCTTCC
CTTCCTTTCT
CGCCACGTTC
GCCGGCTTTC
CCCGTCAAGC
TCTAAATCGG
GGGCTCCCTT
TAGGGTTCCG
ATTTAGTGCT
TTACGGCACC
TCGACCCCAA
AAAACTTGAT
TTGGGTGATG
GTTCACGTAG
TGGGCCATCG
CCCTGATAGA
CGGTTTTTCG
CCCTTTGACG
TTGGAGTCCA
CGTTCTTTAA
TAGTGGACTC
TTGTTCCAAA
CTGGAACAAC
ACTCAACTCT
ATCTCGGGCT
ATTCTTTTGA
TTTATAAGGG
ATTTTGCCGA
TTTCGGTCTA
TTGGTTAAAA
AATGAGCTGA
TTTAACAAAA
ATTTAACGCG
AATTTTAACA
AAATATTAAC
GTTTACAATT
TTATGGTGCA
CTCTCAGTAC
AATCTGCTCT
GATGCCGCAT
AGTTAAGCCA
GCCCCGACAC
CCGCCAACAC
CCGCTGACGC
GCCCTGACGG
GCTTGTCTGC
TCCCGGCATC
CGCTTACAGA
CAAGCTGTGA
CCGTCTCCGG
GAGCTGCATG
TGTCAGAGGT
TTTCACCGTC
ATCACCGAAA
CGCGCGAGAC
GAAAGGGCCT
CGTGATACGC
CTATTTTTAT
AGGTTAATGT
CATGATAATA
ATGGTTTCTT
AGACGTCAGG
TGGCACTTTT
CGGGGAAATG
TGCGCGGAAC
CCCTATTTGT
TTATTTTTCT
AAATACATTC
AAATATGTAT
CCGCTCATGA
GACAATAACC
CTGATAAATG
CTTCAATAAT
ATTGAAAAAG
GAAGAGTATG
AGTATTCAAC
ATTTCCGTGT
CGCCCTTATT
CCCTTTTTTG
CGGCATTTTG
CCTTCCTGTT
TTTGCTCACC
CAGAAACGCT
GGTGAAAGTA
AAAGATGCTG
AAGATCAGTT
GGGTGCACGA
GTGGGTTACA
TCGAACTGGA
TCTCAACAGC
GGTAAGATCC
TTGAGAGTTT
TCGCCCCGAA
GAACGTTTTC
CAATGATGAG
CACTTTTAAA
GTTCTGCTAT
GTGGCGCGGT
ATTATCCCGT
ATTGACGCCG
GGCAAGAGCA
ACTCGGTCGC
CGCATACACT
ATTCTCAGAA
TGACTTGGTT
GAGTACTCAC
CAGTCACAGA
AAAGCATCTT
ACGGATGGCA
TGACAGTAAG
AGAATTATGC
AGTGCTGCCA
TAACCATGAG
TGATAACACT
GCGGCCAACT
TACTTCTGAC
AACGATCGGA
GGACCGAAGG
AGCTAACCGC
TTTTTTGCAC
AACATGGGGG
ATCATGTAAC
TCGCCTTGAT
CGTTGGGAAC
CGGAGCTGAA
TGAAGCCATA
CCAAACGACG
AGCGTGACAC
CACGATGCCT
GTAGCAATGG
CAACAACGTT
GCGCAAACTA
TTAACTGGCG
AACTACTTAC
TCTAGCTTCC
CGGCAACAAT
TAATAGACTG
GATGGAGGCG
GATAAAGTTG
CAGGACCACT
TCTGCGCTCG
GCCCTTCCGG
CTGGCTGGTT
TATTGCTGAT
AAATCTGGAG
CCGGTGAGCG
TGGAAGCCGC
GGTATCATTG
CAGCACTGGG
GCCAGATGGT
AAGCCCTCCC
GTATCGTAGT
TATCTACACG
ACGGGGAGTC
AGGCAACTAT
GGATGAACGA
AATAGACAGA
TCGCTGAGAT
AGGTGCCTCA
CTGATTAAGC
ATTGGTAACT
GTCAGACCAA
GTTTACTCAT
ATATACTTTA
GATTGATTTA
AAACTTCATT
TTTAATTTAA
AAGGATCTAG
GTGAAGATCC
TTTTTGATAA
TCTCATGACC
AAAATCCCTT
AACGTGAGTT
TTCGTTCCAC
TGAGCGTCAG
ACCCCGTAGA
AAAGATCAAA
GGATCTTCTT
GAGATCCTTT
TTTTCTGCGC
GTAATCTGCT
GCTTGCAAAC
AAAAAAACCA
CCGCTACCAG
CGGTGGTTTG
TTTGCCGGAT
CAAGAGCTAC
CAACTCTTTT
TCCGAAGGTA
ACTGGCTTCA
GCAGAGCGCA
GATACCAAAT
ACTGTTCTTC
TAGTGTAGCC
GTAGTTAGGC
CACCACTTCA
AGAACTCTGT
AGCACCGCCT
ACATACCTCG
CTCTGCTAAT
CCTGTTACCA
GTGGCTGCTG
CCAGTGGCGA
TAAGTCGTGT
CTTACCGGGT
TGGACTCAAG
ACGATAGTTA
CCGGATAAGG
CGCAGCGGTC
GGGCTGAACG
GGGGGTTCGT
GCACACAGCC
CAGCTTGGAG
CGAACGACCT
ACACCGAACT
GAGATACCTA
CAGCGTGAGC
TATGAGAAAG
CGCCACGCTT
CCCGAAGGGA
GAAAGGCGGA
CAGGTATCCG
GTAAGCGGCA
GGGTCGGAAC
AGGAGAGCGC
ACGAGGGAGC
TTCCAGGGGG
AAACGCCTGG
TATCTTTATA
GTCCTGTCGG
GTTTCGCCAC
CTCTGACTTG
AGCGTCGATT
TTTGTGATGC
TCGTCAGGGG
GGCGGAGCCT
ATGGAAAAAC
GCCAGCAACG
CGGCCTTTTT
ACGGTTCCTG
GCCTTTTGCT