名称: | pLV-U6-Negative-Control-sgRNA(MS2)-Zeo |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 对照 |
原核抗性: | Amp |
筛选标记: | Zeo |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | U6 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AATTAATTCT
GTGGAATGTG
TGTCAGTTAG
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CAGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCATAGTCC
CGCCCCTAAC
TCCGCCCATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCTGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGGCTT
TTGCAAAAAG
CTCCCGGGAG
CTTGTATATC
CATTTTCGGA
TCTGATCAGC
ACGTGTTGAC
AATTAATCAT
CGGCATAGTA
TATCGGCATA
GTATAATACG
ACAAGGTGAG
GAACTAAACC
ATGGCCAAGT
TGACCAGTGC
CGTTCCGGTG
CTCACCGCGC
GCGACGTCGC
CGGAGCGGTC
GAGTTCTGGA
CCGACCGGCT
CGGGTTCTCC
CGGGACTTCG
TGGAGGACGA
CTTCGCCGGT
GTGGTCCGGG
ACGACGTGAC
CCTGTTCATC
AGCGCGGTCC
AGGACCAGGT
GGTGCCGGAC
AACACCCTGG
CCTGGGTGTG
GGTGCGCGGC
CTGGACGAGC
TGTACGCCGA
GTGGTCGGAG
GTCGTGTCCA
CGAACTTCCG
GGACGCCTCC
GGGCCGGCCA
TGACCGAGAT
CGGCGAGCAG
CCGTGGGGGC
GGGAGTTCGC
CCTGCGCGAC
CCGGCCGGCA
ACTGCGTGCA
CTTCGTGGCC
GAGGAGCAGG
ACTGACACGT
GCTACGAGAT
TTCGATTCCA
CCGCCGCCTT
CTATGAAAGG
TTGGGCTTCG
GAATCGTTTT
CCGGGACGCC
GGCTGGATGA
TCCTCCAGCG
CGGGGATCTC
ATGCTGGAGT
TCTTCGCCCA
CCCCAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGTATAC
CGTCGACCTC
TAGCTAGAGC
TTGGCGTAAT
CATGGTCATA
GCTGTTTCCT
GTGTGAAATT
GTTATCCGCT
CACAATTCCA
CACAACATAC
GAGCCGGAAG
CATAAAGTGT
AAAGCCTGGG
GTGCCTAATG
AGTGAGCTAA
CTCACATTAA
TTGCGTTGCG
CTCACTGCCC
GCTTTCCAGT
CGGGAAACCT
GTCGTGCCAG
CTGCATTAAT
GAATCGGCCA
ACGCGCGGGG
AGAGGCGGTT
TGCGTATTGG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGAAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGACG
TCGACGGATC
GGGAGATCTC
CCGATCCCCT
ATGGTGCACT
CTCAGTACAA
TCTGCTCTGA
TGCCGCATAG
TTAAGCCAGT
ATCTGCTCCC
TGCTTGTGTG
TTGGAGGTCG
CTGAGTAGTG
CGCGAGCAAA
ATTTAAGCTA
CAACAAGGCA
AGGCTTGACC
GACAATTGCA
TGAAGAATCT
GCTTAGGGTT
AGGCGTTTTG
CGCTGCTTCG
CGATGTACGG
GCCAGATATA
CGCGTTGACA
TTGATTATTG
ACTAGTTATT
AATAGTAATC
AATTACGGGG
TCATTAGTTC
ATAGCCCATA
TATGGAGTTC
CGCGTTACAT
AACTTACGGT
AAATGGCCCG
CCTGGCTGAC
CGCCCAACGA
CCCCCGCCCA
TTGACGTCAA
TAATGACGTA
TGTTCCCATA
GTAACGCCAA
TAGGGACTTT
CCATTGACGT
CAATGGGTGG
AGTATTTACG
GTAAACTGCC
CACTTGGCAG
TACATCAAGT
GTATCATATG
CCAAGTACGC
CCCCTATTGA
CGTCAATGAC
GGTAAATGGC
CCGCCTGGCA
TTATGCCCAG
TACATGACCT
TATGGGACTT
TCCTACTTGG
CAGTACATCT
ACGTATTAGT
CATCGCTATT
ACCATGGTGA
TGCGGTTTTG
GCAGTACATC
AATGGGCGTG
GATAGCGGTT
TGACTCACGG
GGATTTCCAA
GTCTCCACCC
CATTGACGTC
AATGGGAGTT
TGTTTTGGCA
CCAAAATCAA
CGGGACTTTC
CAAAATGTCG
TAACAACTCC
GCCCCATTGA
CGCAAATGGG
CGGTAGGCGT
GTACGGTGGG
AGGTCTATAT
AAGCAGCGCG
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGC
TGATCTTCAG
ACCTGGAGGA
GGAGATATGA
GGGACAATTG
GAGAAGTGAA
TTATATAAAT
ATAAAGTAGT
AAAAATTGAA
CCATTAGGAG
TAGCACCCAC
CAAGGCAAAG
AGAAGAGTGG
TGCAGAGAGA
AAAAAGAGCA
GTGGGAATAG
GAGCTTTGTT
CCTTGGGTTC
TTGGGAGCAG
CAGGAAGCAC
TATGGGCGCA
GCGTCAATGA
CGCTGACGGT
ACAGGCCAGA
CAATTATTGT
CTGGTATAGT
GCAGCAGCAG
AACAATTTGC
TGAGGGCTAT
TGAGGCGCAA
CAGCATCTGT
TGCAACTCAC
AGTCTGGGGC
ATCAAGCAGC
TCCAGGCAAG
AATCCTGGCT
GTGGAAAGAT
ACCTAAAGGA
TCAACAGCTC
CTGGGGATTT
GGGGTTGCTC
TGGAAAACTC
ATTTGCACCA
CTGCTGTGCC
TTGGAATGCT
AGTTGGAGTA
ATAAATCTCT
GGAACAGATT
TGGAATCACA
CGACCTGGAT
GGAGTGGGAC
AGAGAAATTA
ACAATTACAC
AAGCTTAATA
CACTCCTTAA
TTGAAGAATC
GCAAAACCAG
CAAGAAAAGA
ATGAACAAGA
ATTATTGGAA
TTAGATAAAT
GGGCAAGTTT
GTGGAATTGG
TTTAACATAA
CAAATTGGCT
GTGGTATATA
AAATTATTCA
TAATGATAGT
AGGAGGCTTG
GTAGGTTTAA
GAATAGTTTT
TGCTGTACTT
TCTATAGTGA
ATAGAGTTAG
GCAGGGATAT
TCACCATTAT
CGTTTCAGAC
CCACCTCCCA
ACCCCGAGGG
GACCCGACAG
GCCCGAAGGA
ATAGAAGAAG
AAGGTGGAGA
GAGAGACAGA
GACAGATCCA
TTCGATTAGT
GAACGGATCG
GCACTGCGTG
CGCCAATTCT
GCAGACAAAT
GGCAGTATTC
ATCCACAATT
TTAAAAGAAA
AGGGGGGATT
GGGGGGTACA
GTGCAGGGGA
AAGAATAGTA
GACATAATAG
CAACAGACAT
ACAAACTAAA
GAATTACAAA
AACAAATTAC
AAAAATTCAA
AATTTTCGGG
TTTATTACAG
GGACAGCAGA
GATCCAGTTT
GGTTAATTAG
CTAGCGAGGG
CCTATTTCCC
ATGATTCCTT
CATATTTGCA
TATACGATAC
AAGGCTGTTA
GAGAGATAAT
TGGAATTAAT
TTGACTGTAA
ACACAAAGAT
ATTAGTACAA
AATACGTGAC
GTAGAAAGTA
ATAATTTCTT
GGGTAGTTTG
CAGTTTTAAA
ATTATGTTTT
AAAATGGACT
ATCATATGCT
TACCGTAACT
TGAAAGTATT
TCGATTTCTT
GGCTTTATAT
ATCTTGTGGA
AAGGACGAAA
CACCGGTATT
CTTAAAACTA
GAGAGGTTTT
AGAGCTAGGC
CAACATGAGG
ATCACCCATG
TCTGCAGGGC
CTAGCAAGTT
AAAATAAGGC
TAGTCCGTTA
TCAACTTGGC
CAACATGAGG
ATCACCCATG
TCTGCAGGGC
CAAGTGGCAC
CGAGTCGGTG
CTTTTTTTGG
ATCCTGCAAA
GATGGATAAA
GTTTTAAACA
GAGAGGAATC
TTTGCAGCTA
ATGGACCTTC
TAGGTCTTGA
AAGGAGTGGG
AATTGGCTCC
GGTGCCCGTC
AGTGGGCAGA
GCGCACATCG
CCCACAGTCC
CCGAGAAGTT
GGGGGGAGGG
GTCGGCAATT
GAACCGGTGC
CTAGAGAAGG
TGGCGCGGGG
TAAACTGGGA
AAGTGATGTC
GTGTACTGGC
TCCGCCTTTT
TCCCGAGGGT
GGGGGAGAAC
CGTATATAAG
TGCAGTAGTC
GCCGTGAACG
TTCTTTTTCG
CAACGGGTTT
GCCGCCAGAA
CACAGGTAAG
TGCCGTGTGT
GGTTCCCGCG
GGCCTGGCCT
CTTTACGGGT
TATGGCCCTT
GCGTGCCTTG
AATTACTTCC
ACCTGGCTGC
AGTACGTGAT
TCTTGATCCC
GAGCTTCGGG
TTGGAAGTGG
GTGGGAGAGT
TCGAGGCCTT
GCGCTTAAGG
AGCCCCTTCG
CCTCGTGCTT
GAGTTGAGGC
CTGGCCTGGG
CGCTGGGGCC
GCCGCGTGCG
AATCTGGTGG
CACCTTCGCG
CCTGTCTCGC
TGCTTTCGAT
AAGTCTCTAG
CCATTTAAAA
TTTTTGATGA
CCTGCTGCGA
CGCTTTTTTT
CTGGCAAGAT
AGTCTTGTAA
ATGCGGGCCA
AGATCTGCAC
ACTGGTATTT
CGGTTTTTGG
GGCCGCGGGC
GGCGACGGGG
CCCGTGCGTC
CCAGCGCACA
TGTTCGGCGA
GGCGGGGCCT
GCGAGCGCGG
CCACCGAGAA
TCGGACGGGG
GTAGTCTCAA
GCTGGCCGGC
CTGCTCTGGT
GCCTGGCCTC
GCGCCGCCGT
GTATCGCCCC
GCCCTGGGCG
GCAAGGCTGG
CCCGGTCGGC
ACCAGTTGCG
TGAGCGGAAA
GATGGCCGCT
TCCCGGCCCT
GCTGCAGGGA
GCTCAAAATG
AAGGACGCGG
CGCTCGGGAG
AGCGGGCGGG
TGAGTCACCC
ACACAAAGGA
AAAGGGCCTT
TCCGTCCTCA
GCCGTCGCTT
CATGTGACTC
CACGGAGTAC
CGGGCGCCGT
CCAGGCACCT
CGATTAGTTC
TCGAGCTTTT
GGAGTACGTC
GTCTTTAGGT
TGGGGGGAGG
GGTTTTATGC
GATGGAGTTT
CCCCACACTG
AGTGGGTGGA
GACTGAAGTT
AGGCCAGCTT
GGCACTTGAT
GTAATTCTCC
TTGGAATTTG
CCCTTTTTGA
GTTTGGATCT
TGGTTCATTC
TCAAGCCTCA
GACAGTGGTT
CAAAGTTTTT
TTCTTCCATT
TCAGGTGTCG
TGATGTACAA
TGGCCAAGTT
GACCAGTGCC
GTTCCGGTGC
TCACCGCGCG
CGACGTCGCC
GGAGCGGTCG
AGTTCTGGAC
CGACCGGCTC
GGGTTCTCCC
GGGACTTCGT
GGAGGACGAC
TTCGCCGGTG
TGGTCCGGGA
CGACGTGACC
CTGTTCATCA
GCGCGGTCCA
GGACCAGGTG
GTGCCGGACA
ACACCCTGGC
CTGGGTGTGG
GTGCGCGGCC
TGGACGAGCT
GTACGCCGAG
TGGTCGGAGG
TCGTGTCCAC
GAACTTCCGG
GACGCCTCCG
GGCCGGCCAT
GACCGAGATC
GGCGAGCAGC
CGTGGGGGCG
GGAGTTCGCC
CTGCGCGACC
CGGCCGGCAA
CTGCGTGCAC
TTCGTGGCCG
AGGAGCAGGA
CTGAGAATTC
GATATCAAGC
TTATCGGTAA
TCAACCTCTG
GATTACAAAA
TTTGTGAAAG
ATTGACTGGT
ATTCTTAACT
ATGTTGCTCC
TTTTACGCTA
TGTGGATACG
CTGCTTTAAT
GCCTTTGTAT
CATGCTATTG
CTTCCCGTAT
GGCTTTCATT
TTCTCCTCCT
TGTATAAATC
CTGGTTGCTG
TCTCTTTATG
AGGAGTTGTG
GCCCGTTGTC
AGGCAACGTG
GCGTGGTGTG
CACTGTGTTT
GCTGACGCAA
CCCCCACTGG
TTGGGGCATT
GCCACCACCT
GTCAGCTCCT
TTCCGGGACT
TTCGCTTTCC
CCCTCCCTAT
TGCCACGGCG
GAACTCATCG
CCGCCTGCCT
TGCCCGCTGC
TGGACAGGGG
CTCGGCTGTT
GGGCACTGAC
AATTCCGTGG
TGTTGTCGGG
GAAATCATCG
TCCTTTCCTT
GGCTGCTCGC
CTGTGTTGCC
ACCTGGATTC
TGCGCGGGAC
GTCCTTCTGC
TACGTCCCTT
CGGCCCTCAA
TCCAGCGGAC
CTTCCTTCCC
GCGGCCTGCT
GCCGGCTCTG
CGGCCTCTTC
CGCGTCTTCG
CCTTCGCCCT
CAGACGAGTC
GGATCTCCCT
TTGGGCCGCC
TCCCCGCATC
GATACCGTCG
ACCTCGAGAC
CTAGAAAAAC
ATGGAGCAAT
CACAAGTAGC
AATACAGCAG
CTACCAATGC
TGATTGTGCC
TGGCTAGAAG
CACAAGAGGA
GGAGGAGGTG
GGTTTTCCAG
TCACACCTCA
GGTACCTTTA
AGACCAATGA
CTTACAAGGC
AGCTGTAGAT
CTTAGCCACT
TTTTAAAAGA
AAAGGGGGGA
CTGGAAGGGC
TAATTCACTC
CCAACGAAGA
CAAGATATCC
TTGATCTGTG
GATCTACCAC
ACACAAGGCT
ACTTCCCTGA
TTGGCAGAAC
TACACACCAG
GGCCAGGGAT
CAGATATCCA
CTGACCTTTG
GATGGTGCTA
CAAGCTAGTA
CCAGTTGAGC
AAGAGAAGGT
AGAAGAAGCC
AATGAAGGAG
AGAACACCCG
CTTGTTACAC
CCTGTGAGCC
TGCATGGGAT
GGATGACCCG
GAGAGAGAAG
TATTAGAGTG
GAGGTTTGAC
AGCCGCCTAG
CATTTCATCA
CATGGCCCGA
GAGCTGCATC
CGGACTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGGGCCCGT
TTAAACCCGC
TGATCAGCCT
CGACTGTGCC
TTCTAGTTGC
CAGCCATCTG
TTGTTTGCCC
CTCCCCCGTG
CCTTCCTTGA
CCCTGGAAGG
TGCCACTCCC
ACTGTCCTTT
CCTAATAAAA
TGAGGAAATT
GCATCGCATT
GTCTGAGTAG
GTGTCATTCT
ATTCTGGGGG
GTGGGGTGGG
GCAGGACAGC
AAGGGGGAGG
ATTGGGAAGA
CAATAGCAGG
CATGCTGGGG
ATGCGGTGGG
CTCTATGGCT
TCTGAGGCGG
AAAGAACCAG
CTGGGGCTCT
AGGGGGTATC
CCCACGCGCC
CTGTAGCGGC
GCATTAAGCG
CGGCGGGTGT
GGTGGTTACG
CGCAGCGTGA
CCGCTACACT
TGCCAGCGCC
CTAGCGCCCG
CTCCTTTCGC
TTTCTTCCCT
TCCTTTCTCG
CCACGTTCGC
CGGCTTTCCC
CGTCAAGCTC
TAAATCGGGG
GCTCCCTTTA
GGGTTCCGAT
TTAGTGCTTT
ACGGCACCTC
GACCCCAAAA
AACTTGATTA
GGGTGATGGT
TCACGTAGTG
GGCCATCGCC
CTGATAGACG
GTTTTTCGCC
CTTTGACGTT
GGAGTCCACG
TTCTTTAATA
GTGGACTCTT
GTTCCAAACT
GGAACAACAC
TCAACCCTAT
CTCGGTCTAT
TCTTTTGATT
TATAAGGGAT
TTTGCCGATT
TCGGCCTATT
GGTTAAAAAA
TGAGCTGATT
TAACAAAAAT
TTAACGCG