名称: | pLenti-PE2-BSD |
---|---|
别称: | Plasmid#161514 |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Blast |
荧光标记: | |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GACATTGATT
ATTGACTAGT
TATTAATAGT
AATCAATTAC
GGGGTCATTA
GTTCATAGCC
CATATATGGA
GTTCCGCGTT
ACATAACTTA
CGGTAAATGG
CCCGCCTGGC
TGACCGCCCA
ACGACCCCCG
CCCATTGACG
TCAATAATGA
CGTATGTTCC
CATAGTAACG
CCAATAGGGA
CTTTCCATTG
ACGTCAATGG
GTGGAGTATT
TACGGTAAAC
TGCCCACTTG
GCAGTACATC
AAGTGTATCA
TATGCCAAGT
ACGCCCCCTA
TTGACGTCAA
TGACGGTAAA
TGGCCCGCCT
GGCATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTCT
ATATAAGCAG
CGCGTTTTGC
CTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGT
GGCGCCCGAA
CAGGGACTTG
AAAGCGAAAG
GGAAACCAGA
GGAGCTCTCT
CGACGCAGGA
CTCGGCTTGC
TGAAGCGCGC
ACGGCAAGAG
GCGAGGGGCG
GCGACTGGTG
AGTACGCCAA
AAATTTTGAC
TAGCGGAGGC
TAGAAGGAGA
GAGATGGGTG
CGAGAGCGTC
AGTATTAAGC
GGGGGAGAAT
TAGATCGCGA
TGGGAAAAAA
TTCGGTTAAG
GCCAGGGGGA
AAGAAAAAAT
ATAAATTAAA
ACATATAGTA
TGGGCAAGCA
GGGAGCTAGA
ACGATTCGCA
GTTAATCCTG
GCCTGTTAGA
AACATCAGAA
GGCTGTAGAC
AAATACTGGG
ACAGCTACAA
CCATCCCTTC
AGACAGGATC
AGAAGAACTT
AGATCATTAT
ATAATACAGT
AGCAACCCTC
TATTGTGTGC
ATCAAAGGAT
AGAGATAAAA
GACACCAAGG
AAGCTTTAGA
CAAGATAGAG
GAAGAGCAAA
ACAAAAGTAA
GACCACCGCA
CAGCAAGCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCGGCACTG
CGTGCGCCAA
TTCTGCAGAC
AAATGGCAGT
ATTCATCCAC
AATTTTAAAA
GAAAAGGGGG
GATTGGGGGG
TACAGTGCAG
GGGAAAGAAT
AGTAGACATA
ATAGCAACAG
ACATACAAAC
TAAAGAATTA
CAAAAACAAA
TTACAAAAAT
TCAAAATTTT
CGGGTTTATT
ACAGGGACAG
CAGAGATCCA
GTTTGGTTAA
TTAGCTAGCT
AGGTCTTGAA
AGGAGTGGGA
ATTGGCTCCG
GTGCCCGTCA
GTGGGCAGAG
CGCACATCGC
CCACAGTCCC
CGAGAAGTTG
GGGGGAGGGG
TCGGCAATTG
ATCCGGTGCC
TAGAGAAGGT
GGCGCGGGGT
AAACTGGGAA
AGTGATGTCG
TGTACTGGCT
CCGCCTTTTT
CCCGAGGGTG
GGGGAGAACC
GTATATAAGT
GCAGTAGTCG
CCGTGAACGT
TCTTTTTCGC
AACGGGTTTG
CCGCCAGAAC
ACAGGACCGG
TTCTAGAGCC
ACCATGAAAC
GGACAGCCGA
CGGAAGCGAG
TTCGAGTCAC
CAAAGAAGAA
GCGGAAAGTC
GACAAGAAGT
ACAGCATCGG
CCTGGACATC
GGCACCAACT
CTGTGGGCTG
GGCCGTGATC
ACCGACGAGT
ACAAGGTGCC
CAGCAAGAAA
TTCAAGGTGC
TGGGCAACAC
CGACCGGCAC
AGCATCAAGA
AGAACCTGAT
CGGAGCCCTG
CTGTTCGACA
GCGGCGAAAC
AGCCGAGGCC
ACCCGGCTGA
AGAGAACCGC
CAGAAGAAGA
TACACCAGAC
GGAAGAACCG
GATCTGCTAT
CTGCAAGAGA
TCTTCAGCAA
CGAGATGGCC
AAGGTGGACG
ACAGCTTCTT
CCACAGACTG
GAAGAGTCCT
TCCTGGTGGA
AGAGGATAAG
AAGCACGAGC
GGCACCCCAT
CTTCGGCAAC
ATCGTGGACG
AGGTGGCCTA
CCACGAGAAG
TACCCCACCA
TCTACCACCT
GAGAAAGAAA
CTGGTGGACA
GCACCGACAA
GGCCGACCTG
CGGCTGATCT
ATCTGGCCCT
GGCCCACATG
ATCAAGTTCC
GGGGCCACTT
CCTGATCGAG
GGCGACCTGA
ACCCCGACAA
CAGCGACGTG
GACAAGCTGT
TCATCCAGCT
GGTGCAGACC
TACAACCAGC
TGTTCGAGGA
AAACCCCATC
AACGCCAGCG
GCGTGGACGC
CAAGGCCATC
CTGTCTGCCA
GACTGAGCAA
GAGCAGACGG
CTGGAAAATC
TGATCGCCCA
GCTGCCCGGC
GAGAAGAAGA
ATGGCCTGTT
CGGAAACCTG
ATTGCCCTGA
GCCTGGGCCT
GACCCCCAAC
TTCAAGAGCA
ACTTCGACCT
GGCCGAGGAT
GCCAAACTGC
AGCTGAGCAA
GGACACCTAC
GACGACGACC
TGGACAACCT
GCTGGCCCAG
ATCGGCGACC
AGTACGCCGA
CCTGTTTCTG
GCCGCCAAGA
ACCTGTCCGA
CGCCATCCTG
CTGAGCGACA
TCCTGAGAGT
GAACACCGAG
ATCACCAAGG
CCCCCCTGAG
CGCCTCTATG
ATCAAGAGAT
ACGACGAGCA
CCACCAGGAC
CTGACCCTGC
TGAAAGCTCT
CGTGCGGCAG
CAGCTGCCTG
AGAAGTACAA
AGAGATTTTC
TTCGACCAGA
GCAAGAACGG
CTACGCCGGC
TACATTGACG
GCGGAGCCAG
CCAGGAAGAG
TTCTACAAGT
TCATCAAGCC
CATCCTGGAA
AAGATGGACG
GCACCGAGGA
ACTGCTCGTG
AAGCTGAACA
GAGAGGACCT
GCTGCGGAAG
CAGCGGACCT
TCGACAACGG
CAGCATCCCC
CACCAGATCC
ACCTGGGAGA
GCTGCACGCC
ATTCTGCGGC
GGCAGGAAGA
TTTTTACCCA
TTCCTGAAGG
ACAACCGGGA
AAAGATCGAG
AAGATCCTGA
CCTTCCGCAT
CCCCTACTAC
GTGGGCCCTC
TGGCCAGGGG
AAACAGCAGA
TTCGCCTGGA
TGACCAGAAA
GAGCGAGGAA
ACCATCACCC
CCTGGAACTT
CGAGGAAGTG
GTGGACAAGG
GCGCTTCCGC
CCAGAGCTTC
ATCGAGCGGA
TGACCAACTT
CGATAAGAAC
CTGCCCAACG
AGAAGGTGCT
GCCCAAGCAC
AGCCTGCTGT
ACGAGTACTT
CACCGTGTAT
AACGAGCTGA
CCAAAGTGAA
ATACGTGACC
GAGGGAATGA
GAAAGCCCGC
CTTCCTGAGC
GGCGAGCAGA
AAAAGGCCAT
CGTGGACCTG
CTGTTCAAGA
CCAACCGGAA
AGTGACCGTG
AAGCAGCTGA
AAGAGGACTA
CTTCAAGAAA
ATCGAGTGCT
TCGACTCCGT
GGAAATCTCC
GGCGTGGAAG
ATCGGTTCAA
CGCCTCCCTG
GGCACATACC
ACGATCTGCT
GAAAATTATC
AAGGACAAGG
ACTTCCTGGA
CAATGAGGAA
AACGAGGACA
TTCTGGAAGA
TATCGTGCTG
ACCCTGACAC
TGTTTGAGGA
CAGAGAGATG
ATCGAGGAAC
GGCTGAAAAC
CTATGCCCAC
CTGTTCGACG
ACAAAGTGAT
GAAGCAGCTG
AAGCGGCGGA
GATACACCGG
CTGGGGCAGG
CTGAGCCGGA
AGCTGATCAA
CGGCATCCGG
GACAAGCAGT
CCGGCAAGAC
AATCCTGGAT
TTCCTGAAGT
CCGACGGCTT
CGCCAACAGA
AACTTCATGC
AGCTGATCCA
CGACGACAGC
CTGACCTTTA
AAGAGGACAT
CCAGAAAGCC
CAGGTGTCCG
GCCAGGGCGA
TAGCCTGCAC
GAGCACATTG
CCAATCTGGC
CGGCAGCCCC
GCCATTAAGA
AGGGCATCCT
GCAGACAGTG
AAGGTGGTGG
ACGAGCTCGT
GAAAGTGATG
GGCCGGCACA
AGCCCGAGAA
CATCGTGATC
GAAATGGCCA
GAGAGAACCA
GACCACCCAG
AAGGGACAGA
AGAACAGCCG
CGAGAGAATG
AAGCGGATCG
AAGAGGGCAT
CAAAGAGCTG
GGCAGCCAGA
TCCTGAAAGA
ACACCCCGTG
GAAAACACCC
AGCTGCAGAA
CGAGAAGCTG
TACCTGTACT
ACCTGCAGAA
TGGGCGGGAT
ATGTACGTGG
ACCAGGAACT
GGACATCAAC
CGGCTGTCCG
ACTACGATGT
GGACGCTATC
GTGCCTCAGA
GCTTTCTGAA
GGACGACTCC
ATCGACAACA
AGGTGCTGAC
CAGAAGCGAC
AAGAACCGGG
GCAAGAGCGA
CAACGTGCCC
TCCGAAGAGG
TCGTGAAGAA
GATGAAGAAC
TACTGGCGGC
AGCTGCTGAA
CGCCAAGCTG
ATTACCCAGA
GAAAGTTCGA
CAATCTGACC
AAGGCCGAGA
GAGGCGGCCT
GAGCGAACTG
GATAAGGCCG
GCTTCATCAA
GAGACAGCTG
GTGGAAACCC
GGCAGATCAC
AAAGCACGTG
GCACAGATCC
TGGACTCCCG
GATGAACACT
AAGTACGACG
AGAATGACAA
GCTGATCCGG
GAAGTGAAAG
TGATCACCCT
GAAGTCCAAG
CTGGTGTCCG
ATTTCCGGAA
GGATTTCCAG
TTTTACAAAG
TGCGCGAGAT
CAACAACTAC
CACCACGCCC
ACGACGCCTA
CCTGAACGCC
GTCGTGGGAA
CCGCCCTGAT
CAAAAAGTAC
CCTAAGCTGG
AAAGCGAGTT
CGTGTACGGC
GACTACAAGG
TGTACGACGT
GCGGAAGATG
ATCGCCAAGA
GCGAGCAGGA
AATCGGCAAG
GCTACCGCCA
AGTACTTCTT
CTACAGCAAC
ATCATGAACT
TTTTCAAGAC
CGAGATTACC
CTGGCCAACG
GCGAGATCCG
GAAGCGGCCT
CTGATCGAGA
CAAACGGCGA
AACCGGGGAG
ATCGTGTGGG
ATAAGGGCCG
GGATTTTGCC
ACCGTGCGGA
AAGTGCTGAG
CATGCCCCAA
GTGAATATCG
TGAAAAAGAC
CGAGGTGCAG
ACAGGCGGCT
TCAGCAAAGA
GTCTATCCTG
CCCAAGAGGA
ACAGCGATAA
GCTGATCGCC
AGAAAGAAGG
ACTGGGACCC
TAAGAAGTAC
GGCGGCTTCG
ACAGCCCCAC
CGTGGCCTAT
TCTGTGCTGG
TGGTGGCCAA
AGTGGAAAAG
GGCAAGTCCA
AGAAACTGAA
GAGTGTGAAA
GAGCTGCTGG
GGATCACCAT
CATGGAAAGA
AGCAGCTTCG
AGAAGAATCC
CATCGACTTT
CTGGAAGCCA
AGGGCTACAA
AGAAGTGAAA
AAGGACCTGA
TCATCAAGCT
GCCTAAGTAC
TCCCTGTTCG
AGCTGGAAAA
CGGCCGGAAG
AGAATGCTGG
CCTCTGCCGG
CGAACTGCAG
AAGGGAAACG
AACTGGCCCT
GCCCTCCAAA
TATGTGAACT
TCCTGTACCT
GGCCAGCCAC
TATGAGAAGC
TGAAGGGCTC
CCCCGAGGAT
AATGAGCAGA
AACAGCTGTT
TGTGGAACAG
CACAAGCACT
ACCTGGACGA
GATCATCGAG
CAGATCAGCG
AGTTCTCCAA
GAGAGTGATC
CTGGCCGACG
CTAATCTGGA
CAAAGTGCTG
TCCGCCTACA
ACAAGCACCG
GGATAAGCCC
ATCAGAGAGC
AGGCCGAGAA
TATCATCCAC
CTGTTTACCC
TGACCAATCT
GGGAGCCCCT
GCCGCCTTCA
AGTACTTTGA
CACCACCATC
GACCGGAAGA
GGTACACCAG
CACCAAAGAG
GTGCTGGACG
CCACCCTGAT
CCACCAGAGC
ATCACCGGCC
TGTACGAGAC
ACGGATCGAC
CTGTCTCAGC
TGGGAGGTGA
CTCTGGAGGA
TCTAGCGGAG
GATCCTCTGG
CAGCGAGACA
CCAGGAACAA
GCGAGTCAGC
AACACCAGAG
AGCAGTGGCG
GCAGCAGCGG
CGGCAGCAGC
ACCCTAAATA
TAGAAGATGA
GTATCGGCTA
CATGAGACCT
CAAAAGAGCC
AGATGTTTCT
CTAGGGTCCA
CATGGCTGTC
TGATTTTCCT
CAGGCCTGGG
CGGAAACCGG
GGGCATGGGA
CTGGCAGTTC
GCCAAGCTCC
TCTGATCATA
CCTCTGAAAG
CAACCTCTAC
CCCCGTGTCC
ATAAAACAAT
ACCCCATGTC
ACAAGAAGCC
AGACTGGGGA
TCAAGCCCCA
CATACAGAGA
CTGTTGGACC
AGGGAATACT
GGTACCCTGC
CAGTCCCCCT
GGAACACGCC
CCTGCTACCC
GTTAAGAAAC
CAGGGACTAA
TGATTATAGG
CCTGTCCAGG
ATCTGAGAGA
AGTCAACAAG
CGGGTGGAAG
ACATCCACCC
CACCGTGCCC
AACCCTTACA
ACCTCTTGAG
CGGGCTCCCA
CCGTCCCACC
AGTGGTACAC
TGTGCTTGAT
TTAAAGGATG
CCTTTTTCTG
CCTGAGACTC
CACCCCACCA
GTCAGCCTCT
CTTCGCCTTT
GAGTGGAGAG
ATCCAGAGAT
GGGAATCTCA
GGACAATTGA
CCTGGACCAG
ACTCCCACAG
GGTTTCAAAA
ACAGTCCCAC
CCTGTTTAAT
GAGGCACTGC
ACAGAGACCT
AGCAGACTTC
CGGATCCAGC
ACCCAGACTT
GATCCTGCTA
CAGTACGTGG
ATGACTTACT
GCTGGCCGCC
ACTTCTGAGC
TAGACTGCCA
ACAAGGTACT
CGGGCCCTGT
TACAAACCCT
AGGGAACCTC
GGGTATCGGG
CCTCGGCCAA
GAAAGCCCAA
ATTTGCCAGA
AACAGGTCAA
GTATCTGGGG
TATCTTCTAA
AAGAGGGTCA
GAGATGGCTG
ACTGAGGCCA
GAAAAGAGAC
TGTGATGGGG
CAGCCTACTC
CGAAGACCCC
TCGACAACTA
AGGGAGTTCC
TAGGGAAGGC
AGGCTTCTGT
CGCCTCTTCA
TCCCTGGGTT
TGCAGAAATG
GCAGCCCCCC
TGTACCCTCT
CACCAAACCG
GGGACTCTGT
TTAATTGGGG
CCCAGACCAA
CAAAAGGCCT
ATCAAGAAAT
CAAGCAAGCT
CTTCTAACTG
CCCCAGCCCT
GGGGTTGCCA
GATTTGACTA
AGCCCTTTGA
ACTCTTTGTC
GACGAGAAGC
AGGGCTACGC
CAAAGGTGTC
CTAACGCAAA
AACTGGGACC
TTGGCGTCGG
CCGGTGGCCT
ACCTGTCCAA
AAAGCTAGAC
CCAGTAGCAG
CTGGGTGGCC
CCCTTGCCTA
CGGATGGTAG
CAGCCATTGC
CGTACTGACA
AAGGATGCAG
GCAAGCTAAC
CATGGGACAG
CCACTAGTCA
TTCTGGCCCC
CCATGCAGTA
GAGGCACTAG
TCAAACAACC
CCCCGACCGC
TGGCTTTCCA
ACGCCCGGAT
GACTCACTAT
CAGGCCTTGC
TTTTGGACAC
GGACCGGGTC
CAGTTCGGAC
CGGTGGTAGC
CCTGAACCCG
GCTACGCTGC
TCCCACTGCC
TGAGGAAGGG
CTGCAACACA
ACTGCCTTGA
TATCCTGGCC
GAAGCCCACG
GAACCCGACC
CGACCTAACG
GACCAGCCGC
TCCCAGACGC
CGACCACACC
TGGTACACGG
ATGGAAGCAG
TCTCTTACAA
GAGGGACAGC
GTAAGGCGGG
AGCTGCGGTG
ACCACCGAGA
CCGAGGTAAT
CTGGGCTAAA
GCCCTGCCAG
CCGGGACATC
CGCTCAGCGG
GCTGAACTGA
TAGCACTCAC
CCAGGCCCTA
AAGATGGCAG
AAGGTAAGAA
GCTAAATGTT
TATACTGATA
GCCGTTATGC
TTTTGCTACT
GCCCATATCC
ATGGAGAAAT
ATACAGAAGG
CGTGGGTGGC
TCACATCAGA
AGGCAAAGAG
ATCAAAAATA
AAGACGAGAT
CTTGGCCCTA
CTAAAAGCCC
TCTTTCTGCC
CAAAAGACTT
AGCATAATCC
ATTGTCCAGG
ACATCAAAAG
GGACACAGCG
CCGAGGCTAG
AGGCAACCGG
ATGGCTGACC
AAGCGGCCCG
AAAGGCAGCC
ATCACAGAGA
CTCCAGACAC
CTCTACCCTC
CTCATAGAAA
ATTCATCACC
CTCTGGCGGC
TCAAAAAGAA
CCGCCGACGG
CAGCGAATTC
GAGCCCAAGA
AGAAGAGGAA
AGTCGGATCC
GGCGCAACAA
ACTTCTCTCT
GCTGAAACAA
GCCGGAGATG
TCGAAGAGAA
TCCTGGACCG
ATGGCCAAGC
CTTTGTCTCA
AGAAGAATCC
ACCCTCATTG
AAAGAGCAAC
GGCTACAATC
AACAGCATCC
CCATCTCTGA
AGACTACAGC
GTCGCCAGCG
CAGCTCTCTC
TAGCGACGGC
CGCATCTTCA
CTGGTGTCAA
TGTATATCAT
TTTACTGGGG
GACCTTGTGC
AGAACTCGTG
GTGCTGGGCA
CTGCTGCTGC
TGCGGCAGCT
GGCAACCTGA
CTTGTATCGT
CGCGATCGGA
AATGAGAACA
GGGGCATCTT
GAGCCCCTGC
GGACGGTGCC
GACAGGTGCT
TCTCGATCTG
CATCCTGGGA
TCAAAGCCAT
AGTGAAGGAC
AGTGATGGAC
AGCCGACGGC
AGTTGGGATT
CGTGAATTGC
TGCCCTCTGG
TTATGTGTGG
GAGGGCTAAG
AATTCGATAT
CAAGCTTATC
GGTAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAAT
CATCGTCCTT
TCCTTGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCATCGATAC
CGTCGACCTC
GAGACCTAGA
AAAACATGGA
GCAATCACAA
GTAGCAATAC
AGCAGCTACC
AATGCTGATT
GTGCCTGGCT
AGAAGCACAA
GAGGAGGAGG
AGGTGGGTTT
TCCAGTCACA
CCTCAGGTAC
CTTTAAGACC
AATGACTTAC
AAGGCAGCTG
TAGATCTTAG
CCACTTTTTA
AAAGAAAAGG
GGGGACTGGA
AGGGCTAATT
CACTCCCAAC
GAAGACAAGA
TATCCTTGAT
CTGTGGATCT
ACCACACACA
AGGCTACTTC
CCTGATTGGC
AGAACTACAC
ACCAGGGCCA
GGGATCAGAT
ATCCACTGAC
CTTTGGATGG
TGCTACAAGC
TAGTACCAGT
TGAGCAAGAG
AAGGTAGAAG
AAGCCAATGA
AGGAGAGAAC
ACCCGCTTGT
TACACCCTGT
GAGCCTGCAT
GGGATGGATG
ACCCGGAGAG
AGAAGTATTA
GAGTGGAGGT
TTGACAGCCG
CCTAGCATTT
CATCACATGG
CCCGAGAGCT
GCATCCGGAC
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGGG
CCCGTTTAAA
CCCGCTGATC
AGCCTCGACT
GTGCCTTCTA
GTTGCCAGCC
ATCTGTTGTT
TGCCCCTCCC
CCGTGCCTTC
CTTGACCCTG
GAAGGTGCCA
CTCCCACTGT
CCTTTCCTAA
TAAAATGAGG
AAATTGCATC
GCATTGTCTG
AGTAGGTGTC
ATTCTATTCT
GGGGGGTGGG
GTGGGGCAGG
ACAGCAAGGG
GGAGGATTGG
GAAGACAATA
GCAGGCATGC
TGGGGATGCG
GTGGGCTCTA
TGGCTTCTGA
GGCGGAAAGA
ACCAGCTGGG
GCTCTAGGGG
GTATCCCCAC
GCGCCCTGTA
GCGGCGCATT
AAGCGCGGCG
GGTGTGGTGG
TTACGCGCAG
CGTGACCGCT
ACACTTGCCA
GCGCCCTAGC
GCCCGCTCCT
TTCGCTTTCT
TCCCTTCCTT
TCTCGCCACG
TTCGCCGGCT
TTCCCCGTCA
AGCTCTAAAT
CGGGGGCTCC
CTTTAGGGTT
CCGATTTAGT
GCTTTACGGC
ACCTCGACCC
CAAAAAACTT
GATTAGGGTG
ATGGTTCACG
TAGTGGGCCA
TCGCCCTGAT
AGACGGTTTT
TCGCCCTTTG
ACGTTGGAGT
CCACGTTCTT
TAATAGTGGA
CTCTTGTTCC
AAACTGGAAC
AACACTCAAC
CCTATCTCGG
TCTATTCTTT
TGATTTATAA
GGGATTTTGC
CGATTTCGGC
CTATTGGTTA
AAAAATGAGC
TGATTTAACA
AAAATTTAAC
GCGAATTAAT
TCTGTGGAAT
GTGTGTCAGT
TAGGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCAGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCATAG
TCCCGCCCCT
AACTCCGCCC
AGTTCCGCCC
ATTCTCCGCC
CCATGGCTGA
CTAATTTTTT
TTATTTATGC
AGAGGCCGAG
GCCGCCTCTG
CCTCTGAGCT
ATTCCAGAAG
TAGTGAGGAG
GCTTTTTTGG
AGGCCTAGGC
TTTTGCAAAA
AGCTCCCGGG
AGCTTGTATA
TCCATTTTCG
GATCTGATCA
GCACGTGTTG
ACAATTAATC
ATCGGCATAG
TATATCGGCA
TAGTATAATA
CGACAAGGTG
AGGAACTAAA
CCATGGCCAA
GTTGACCAGT
GCCGTTCCGG
TGCTCACCGC
GCGCGACGTC
GCCGGAGCGG
TCGAGTTCTG
GACCGACCGG
CTCGGGTTCT
CCCGGGACTT
CGTGGAGGAC
GACTTCGCCG
GTGTGGTCCG
GGACGACGTG
ACCCTGTTCA
TCAGCGCGGT
CCAGGACCAG
GTGGTGCCGG
ACAACACCCT
GGCCTGGGTG
TGGGTGCGCG
GCCTGGACGA
GCTGTACGCC
GAGTGGTCGG
AGGTCGTGTC
CACGAACTTC
CGGGACGCCT
CCGGGCCGGC
CATGACCGAG
ATCGGCGAGC
AGCCGTGGGG
GCGGGAGTTC
GCCCTGCGCG
ACCCGGCCGG
CAACTGCGTG
CACTTCGTGG
CCGAGGAGCA
GGACTGACAC
GTGCTACGAG
ATTTCGATTC
CACCGCCGCC
TTCTATGAAA
GGTTGGGCTT
CGGAATCGTT
TTCCGGGACG
CCGGCTGGAT
GATCCTCCAG
CGCGGGGATC
TCATGCTGGA
GTTCTTCGCC
CACCCCAACT
TGTTTATTGC
AGCTTATAAT
GGTTACAAAT
AAAGCAATAG
CATCACAAAT
TTCACAAATA
AAGCATTTTT
TTCACTGCAT
TCTAGTTGTG
GTTTGTCCAA
ACTCATCAAT
GTATCTTATC
ATGTCTGTAT
ACCGTCGACC
TCTAGCTAGA
GCTTGGCGTA
ATCATGGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTG
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGA
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGTCGACGGA
TCGGGAGATC
TCCCGATCCC
CTATGGTGCA
CTCTCAGTAC
AATCTGCTCT
GATGCCGCAT
AGTTAAGCCA
GTATCTGCTC
CCTGCTTGTG
TGTTGGAGGT
CGCTGAGTAG
TGCGCGAGCA
AAATTTAAGC
TACAACAAGG
CAAGGCTTGA
CCGACAATTG
CATGAAGAAT
CTGCTTAGGG
TTAGGCGTTT
TGCGCTGCTT
CGCGATGTAC
GGGCCAGATA
TACGCGTT