名称: | pGBKT7-SCARB2(human) |
---|---|
别称: | NM_005506.4;AMRF; CD36L2; EPM4; HLGP85; LGP85; LIMP-2;LIMPII; SR-BII |
基因长: | 1437bp |
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Kan |
筛选标记: | TRP1 |
荧光标记: | |
启动子: | ADH1 |
复制子: | 2U |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGGTGCGGGC
CTCTTCGCTA
TTACGCCAGA
TCCTTTTGTT
GTTTCCGGGT
GTACAATATG
GACTTCCTCT
TTTCTGGCAA
CCAAACCCAT
ACATCGGGAT
TCCTATAATA
CCTTCGTTGG
TCTCCCTAAC
ATGTAGGTGG
CGGAGGGGAG
ATATACAATA
GAACAGATAC
CAGACAAGAC
ATAATGGGCT
AAACAAGACT
ACACCAATTA
CACTGCCTCA
TTGATGGTGG
TACATAACGA
ACTAATACTG
TAGCCCTAGA
CTTGATAGCC
ATCATCATAT
CGAAGTTTCA
CTACCCTTTT
TCCATTTGCC
ATCTATTGAA
GTAATAATAG
GCGCATGCAA
CTTCTTTTCT
TTTTTTTTCT
TTTCTCTCTC
CCCCGTTGTT
GTCTCACCAT
ATCCGCAATG
ACAAAAAAAT
GATGGAAGAC
ACTAAAGGAA
AAAATTAACG
ACAAAGACAG
CACCAACAGA
TGTCGTTGTT
CCAGAGCTGA
TGAGGGGTAT
CTCGAAGCAC
ACGAAACTTT
TTCCTTCCTT
CATTCACGCA
CACTACTCTC
TAATGAGCAA
CGGTATACGG
CCTTCCTTCC
AGTTACTTGA
ATTTGAAATA
AAAAAAAGTT
TGCTGTCTTG
CTATCAAGTA
TAAATAGACC
TGCAATTATT
AATCTTTTGT
TTCCTCGTCA
TTGTTCTCGT
TCCCTTTCTT
CCTTGTTTCT
TTTTCTGCAC
AATATTTCAA
GCTATACCAA
GCATACAATC
AACTCCAAGC
TTGAAGCAAG
CCTCCTGAAA
GATGAAGCTA
CTGTCTTCTA
TCGAACAAGC
ATGCGATATT
TGCCGACTTA
AAAAGCTCAA
GTGCTCCAAA
GAAAAACCGA
AGTGCGCCAA
GTGTCTGAAG
AACAACTGGG
AGTGTCGCTA
CTCTCCCAAA
ACCAAAAGGT
CTCCGCTGAC
TAGGGCACAT
CTGACAGAAG
TGGAATCAAG
GCTAGAAAGA
CTGGAACAGC
TATTTCTACT
GATTTTTCCT
CGAGAAGACC
TTGACATGAT
TTTGAAAATG
GATTCTTTAC
AGGATATAAA
AGCATTGTTA
ACAGGATTAT
TTGTACAAGA
TAATGTGAAT
AAAGATGCCG
TCACAGATAG
ATTGGCTTCA
GTGGAGACTG
ATATGCCTCT
AACATTGAGA
CAGCATAGAA
TAAGTGCGAC
ATCATCATCG
GAAGAGAGTA
GTAACAAAGG
TCAAAGACAG
TTGACTGTAT
CGCCGGAATT
TGTAATACGA
CTCACTATAG
GGCGAGCCGC
CATCATGGAG
GAGCAGAAGC
TGATCTCAGA
GGAGGACCTG
CATATGGCCA
TGGAGGCCGA
ATTCATGGGC
CGATGCTGCT
TCTACACGGC
GGGGACGTTG
TCCCTGCTCC
TGCTGGTGAC
CAGCGTCACG
CTGCTGGTGG
CCCGGGTCTT
CCAGAAGGCT
GTAGACCAGA
GTATCGAGAA
GAAAATTGTG
TTAAGGAATG
GTACTGAGGC
ATTTGACTCC
TGGGAGAAGC
CCCCTCTGCC
TGTGTATACT
CAGTTCTATT
TCTTCAATGT
CACCAATCCA
GAGGAGATCC
TCAGAGGGGA
GACCCCTCGG
GTGGAAGAAG
TGGGGCCATA
CACCTACAGG
GAACTCAGAA
ACAAAGCAAA
TATTCAATTT
GGAGATAATG
GAACAACAAT
ATCTGCTGTT
AGCAACAAGG
CCTATGTTTT
TGAACGAGAC
CAATCTGTTG
GAGACCCTAA
AATTGACTTA
ATTAGAACAT
TAAATATTCC
TGTATTGACT
GTCATAGAGT
GGTCCCAGGT
GCACTTCCTC
AGGGAGATCA
TCGAGGCCAT
GTTGAAAGCC
TATCAGCAGA
AGCTCTTTGT
GACTCACACA
GTTGACGAAT
TGCTCTGGGG
CTACAAAGAT
GAAATCTTGT
CCCTTATCCA
TGTTTTCAGG
CCCGATATCT
CTCCCTATTT
TGGCCTATTC
TATGAGAAAA
ATGGGACTAA
TGATGGAGAC
TATGTTTTTC
TAACTGGAGA
AGACAGTTAC
CTTAACTTTA
CAAAAATTGT
GGAATGGAAT
GGGAAAACGT
CACTTGACTG
GTGGATAACA
GACAAGTGCA
ATATGATTAA
TGGAACAGAT
GGAGATTCTT
TTCACCCACT
AATAACCAAA
GATGAGGTCC
TTTATGTCTT
CCCATCTGAC
TTTTGCAGGT
CAGTGTATAT
TACTTTCAGT
GACTATGAGA
GTGTACAGGG
ACTGCCTGCC
TTTCGGTATA
AAGTTCCTGC
AGAAATATTA
GCCAATACGT
CAGACAATGC
CGGCTTCTGT
ATACCTGAGG
GAAACTGCCT
GGGCTCAGGA
GTTCTGAATG
TCAGCATCTG
CAAGAATGGT
GCACCCATCA
TTATGTCTTT
CCCACACTTT
TACCAAGCAG
ATGAGAGGTT
TGTTTCTGCC
ATAGAAGGCA
TGCACCCAAA
TCAGGAAGAC
CATGAGACAT
TTGTGGACAT
TAATCCTTTG
ACTGGAATAA
TCCTAAAAGC
AGCCAAGAGG
TTCCAAATCA
ACATTTATGT
CAAAAAATTA
GATGACTTTG
TTGAAACGGG
AGACATTAGA
ACCATGGTTT
TCCCAGTGAT
GTACCTCAAT
GAGAGTGTTC
ACATTGATAA
AGAGACGGCG
AGTCGACTGA
AGTCTATGAT
TAACACTACT
TTGATCATCA
CCAACATACC
CTACATCATC
ATGGCGCTGG
GTGTGTTCTT
TGGTTTGGTT
TTTACCTGGC
TTGCATGCAA
AGGACAGGGA
TCCATGGATG
AGGGAACAGC
GGATGAAAGA
GCACCCCTCA
TTCGAACCTA
AGGATCCGTC
GACCTGCAGC
GGCCGCATAA
CTAGCATAAC
CCCTTGGGGC
CTCTAAACGG
GTCTTGAGGG
GTTTTTTGCG
CGCTTGCAGC
CAAGCTAATT
CCGGGCGAAT
TTCTTATGAT
TTATGATTTT
TATTATTAAA
TAAGTTATAA
AAAAAATAAG
TGTATACAAA
TTTTAAAGTG
ACTCTTAGGT
TTTAAAACGA
AAATTCTTAT
TCTTGAGTAA
CTCTTTCCTG
TAGGTCAGGT
TGCTTTCTCA
GGTATAGCAT
GAGGTCGCTC
TTATTGACCA
CACCTCTACC
GGCATGCAAG
CTTGGCGTAA
TCATGGTCAT
AGCTGTTTCC
TGTGTGAAAT
TGTTATCCGC
TCACAATTCC
ACACAACATA
CGAGCCGGAA
GCATAAAGTG
TAAAGCCTGG
GGTGCCTAAT
GAGTGAGCTA
ACTCACATTA
ATTGCGTTGC
GCTCACTGCC
CGCTTTCCAG
TCGGGAAACC
TGTCGTGCCA
GCTGCATTAA
TGAATCGGCC
AACGCGCGGG
GAGAGGCGGT
TTGCGTATTG
GGCGCTCTTC
CGCTTCCTCG
CTCACTGACT
CGCTGCGCTC
GGTCGTTCGG
CTGCGGCGAG
CGGTATCAGC
TCACTCAAAG
GCGGTAATAC
GGTTATCCAC
AGAATCAGGG
GATAACGCAG
GAAAGAACAT
GTGAGCAAAA
GGCCAGCAAA
AGGCCAGGAA
CCGTAAAAAG
GCCGCGTTGC
TGGCGTTTTT
CCATAGGCTC
CGCCCCCCTG
ACGAGCATCA
CAAAAATCGA
CGCTCAAGTC
AGAGGTGGCG
AAACCCGACA
GGACTATAAA
GATACCAGGC
GTTTCCCCCT
GGAAGCTCCC
TCGTGCGCTC
TCCTGTTCCG
ACCCTGCCGC
TTACCGGATA
CCTGTCCGCC
TTTCTCCCTT
CGGGAAGCGT
GGCGCTTTCT
CATAGCTCAC
GCTGTAGGTA
TCTCAGTTCG
GTGTAGGTCG
TTCGCTCCAA
GCTGGGCTGT
GTGCACGAAC
CCCCCGTTCA
GCCCGACCGC
TGCGCCTTAT
CCGGTAACTA
TCGTCTTGAG
TCCAACCCGG
TAAGACACGA
CTTATCGCCA
CTGGCAGCAG
CCACTGGTAA
CAGGATTAGC
AGAGCGAGGT
ATGTAGGCGG
TGCTACAGAG
TTCTTGAAGT
GGTGGCCTAA
CTACGGCTAC
ACTAGAAGAA
CAGTATTTGG
TATCTGCGCT
CTGCTGAAGC
CAGTTACCTT
CGGAAAAAGA
GTTGGTAGCT
CTTGATCCGG
CAAACAAACC
ACCGCTGGTA
GCGGTGGTTT
TTTTGTTTGC
AAGCAGCAGA
TTACGCGCAG
AAAAAAAGGA
TCTCAAGAAG
ATCCTTTGAT
CTTTTCTACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GAGATTATCA
AAAAGGATCT
TCACCTAGAT
CCTTTTAAAT
TAAAAATGAA
GTTTTAAATC
AATCTAAAGT
ATATATGAGT
AACCTGAGGC
TATGGCAGGG
CCTGCCGCCC
CGACGTTGGC
TGCGAGCCCT
GGGCCTTCAC
CCGAACTTGG
GGGGTGGGGT
GGGGAAAAGG
AAGAAACGCG
GGCGTATTGG
CCCCAATGGG
GTCTCGGTGG
GGTATCGACA
GAGTGCCAGC
CCTGGGACCG
AACCCCGCGT
TTATGAACAA
ACGACCCAAC
ACCGTGCGTT
TTATTCTGTC
TTTTTATTGC
CGTCATAGCG
CGGGTTCCTT
CCGGTATTGT
CTCCTTCCGT
GTTTCAGTTA
GCCTCCCCCT
AGGGTGGGCG
AAGAACTCCA
GCATGAGATC
CCCGCGCTGG
AGGATCATCC
AGCCGGCGTC
CCGGAAAACG
ATTCCGAAGC
CCAACCTTTC
ATAGAAGGCG
GCGGTGGAAT
CGAAATCTCG
TGATGGCAGG
TTGGGCGTCG
CTTGGTCGGT
CATTTCGAAC
CCCAGAGTCC
CGCTCAGAAG
AACTCGTCAA
GAAGGCGATA
GAAGGCGATG
CGCTGCGAAT
CGGGAGCGGC
GATACCGTAA
AGCACGAGGA
AGCGGTCAGC
CCATTCGCCG
CCAAGCTCTT
CAGCAATATC
ACGGGTAGCC
AACGCTATGT
CCTGATAGCG
GTCCGCCACA
CCCAGCCGGC
CACAGTCGAT
GAATCCAGAA
AAGCGGCCAT
TTTCCACCAT
GATATTCGGC
AAGCAGGCAT
CGCCATGAGT
CACGACGAGA
TCCTCGCCGT
CGGGCATGCT
CGCCTTGAGC
CTGGCGAACA
GTTCGGCTGG
CGCGAGCCCC
TGATGCTCTT
CGTCCAGATC
ATCCTGATCG
ACAAGACCGG
CTTCCATCCG
AGTACGTGCT
CGCTCGATGC
GATGTTTCGC
TTGGTGGTCG
AATGGGCAGG
TAGCCGGATC
AAGCGTATGC
AGCCGCCGCA
TTGCATCAGC
CATGATGGAT
ACTTTCTCGG
CAGGAGCAAG
GTGAGATGAC
AGGAGATCCT
GCCCCGGCAC
TTCGCCCAAT
AGCAGCCAGT
CCCTTCCCGC
TTCAGTGACA
ACGTCGAGCA
CAGCTGCGCA
AGGAACGCCC
GTCGTGGCCA
GCCACGATAG
CCGCGCTGCC
TCGTCTTGCA
GTTCATTCAG
GGCACCGGAC
AGGTCGGTCT
TGACAAAAAG
AACCGGGCGC
CCCTGCGCTG
ACAGCCGGAA
CACGGCGGCA
TCAGAGCAGC
CGATTGTCTG
TTGTGCCCAG
TCATAGCCGA
ATAGCCTCTC
CACCCAAGCG
GCCGGAGAAC
CTGCGTGCAA
TCCATCTTGT
TCAATCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
ACGAAGCATC
TGTGCTTCAT
TTTGTAGAAC
AAAAATGCAA
CGCGAGAGCG
CTAATTTTTC
AAACAAAGAA
TCTGAGCTGC
ATTTTTACAG
AACAGAAATG
CAACGCGAAA
GCGCTATTTT
ACCAACGAAG
AATCTGTGCT
TCATTTTTGT
AAAACAAAAA
TGCAACGCGA
GAGCGCTAAT
TTTTCAAACA
AAGAATCTGA
GCTGCATTTT
TACAGAACAG
AAATGCAACG
CGAGAGCGCT
ATTTTACCAA
CAAAGAATCT
ATACTTCTTT
TTTGTTCTAC
AAAAATGCAT
CCCGAGAGCG
CTATTTTTCT
AACAAAGCAT
CTTAGATTAC
TTTTTTTCTC
CTTTGTGCGC
TCTATAATGC
AGTCTCTTGA
TAACTTTTTG
CACTGTAGGT
CCGTTAAGGT
TAGAAGAAGG
CTACTTTGGT
GTCTATTTTC
TCTTCCATAA
AAAAAGCCTG
ACTCCACTTC
CCGCGTTTAC
TGATTACTAG
CGAAGCTGCG
GGTGCATTTT
TTCAAGATAA
AGGCATCCCC
GATTATATTC
TATACCGATG
TGGATTGCGC
ATACTTTGTG
AACAGAAAGT
GATAGCGTTG
ATGATTCTTC
ATTGGTCAGA
AAATTATGAA
CGGTTTCTTC
TATTTTGTCT
CTATATACTA
CGTATAGGAA
ATGTTTACAT
TTTCGTATTG
TTTTCGATTC
ACTCTATGAA
TAGTTCTTAC
TACAATTTTT
TTGTCTAAAG
AGTAATACTA
GAGATAAACA
TAAAAAATGT
AGAGGTCGAG
TTTAGATGCA
AGTTCAAGGA
GCGAAAGGTG
GATGGGTAGG
TTATATAGGG
ATATAGCACA
GAGATATATA
GCAAAGAGAT
ACTTTTGAGC
AATGTTTGTG
GAAGCGGTAT
TCGCAATATT
TTAGTAGCTC
GTTACAGTCC
GGTGCGTTTT
TGGTTTTTTG
AAAGTGCGTC
TTCAGAGCGC
TTTTGGTTTT
CAAAAGCGCT
CTGAAGTTCC
TATACTTTCT
AGAGAATAGG
AACTTCGGAA
TAGGAACTTC
AAAGCGTTTC
CGAAAACGAG
CGCTTCCGAA
AATGCAACGC
GAGCTGCGCA
CATACAGCTC
ACTGTTCACG
TCGCACCTAT
ATCTGCGTGT
TGCCTGTATA
TATATATACA
TGAGAAGAAC
GGCATAGTGC
GTGTTTATGC
TTAAATGCGT
ACTTATATGC
GTCTATTTAT
GTAGGATGAA
AGGTAGTCTA
GTACCTCCTG
TGATATTATC
CCATTCCATG
CGGGGTATCG
TATGCTTCCT
TCAGCACTAC
CCTTTAGCTG
TTCTATATGC
TGCCACTCCT
CAATTGGATT
AGTCTCATCC
TTCAATGCTA
TCATTTCCTT
TGATATTGGA
TCATACTAAG
AAACCATTAT
TATCATGACA
TTAACCTATA
AAAATAGGCG
TATCACGAGG
CCCTTTCGTC
TCGCGCGTTT
CGGTGATGAC
GGTGAAAACC
TCTGACACAT
GCAGCTCCCG
GAGACGGTCA
CAGCTTGTCT
GTAAGCGGAT
GCCGGGAGCA
GACAAGCCCG
TCAGGGCGCG
TCAGCGGGTG
TTGGCGGGTG
TCGGGGCTGG
CTTAACTATG
CGGCATCAGA
GCAGATTGTA
CTGAGAGTGC
ACCATAGATC
AACGACATTA
CTATATATAT
AATATAGGAA
GCATTTAATA
GAACAGCATC
GTAATATATG
TGTACTTTGC
AGTTATGACG
CCAGATGGCA
GTAGTGGAAG
ATATTCTTTA
TTGAAAAATA
GCTTGTCACC
TTACGTACAA
TCTTGATCCG
GAGCTTTTCT
TTTTTTGCCG
ATTAAGAATT
AATTCGGTCG
AAAAAAGAAA
AGGAGAGGGC
CAAGAGGGAG
GGCATTGGTG
ACTATTGAGC
ACGTGAGTAT
ACGTGATTAA
GCACACAAAG
GCAGCTTGGA
GTATGTCTGT
TATTAATTTC
ACAGGTAGTT
CTGGTCCATT
GGTGAAAGTT
TGCGGCTTGC
AGAGCACAGA
GGCCGCAGAA
TGTGCTCTAG
ATTCCGATGC
TGACTTGCTG
GGTATTATAT
GTGTGCCCAA
TAGAAAGAGA
ACAATTGACC
CGGTTATTGC
AAGGAAAATT
TCAAGTCTTG
TAAAAGCATA
TAAAAATAGT
TCAGGCACTC
CGAAATACTT
GGTTGGCGTG
TTTCGTAATC
AACCTAAGGA
GGATGTTTTG
GCTCTGGTCA
ATGATTACGG
CATTGATATC
GTCCAACTGC
ATGGAGATGA
GTCGTGGCAA
GAATACCAAG
AGTTCCTCGG
TTTGCCAGTT
ATTAAAAGAC
TCGTATTTCC
AAAAGACTGC
AACATACTAC
TCAGTGCAGC
TTCACAGAAA
CCTCATTCGT
TTATTCCCTT
GTTTGATTCA
GAAGCAGGTG
GGACAGGTGA
ACTTTTGGAT
TGGAACTCGA
TTTCTGACTG
GGTTGGAAGG
CAAGAGAGCC
CCGAAAGCTT
ACATTTTATG
TTAGCTGGTG
GACTGACGCC
AGAAAATGTT
GGTGATGCGC
TTAGATTAAA
TGGCGTTATT
GGTGTTGATG
TAAGCGGAGG
TGTGGAGACA
AATGGTGTAA
AAGACTCTAA
CAAAATAGCA
AATTTCGTCA
AAAATGCTAA
GAAATAGGTT
ATTACTGAGT
AGTATTTATT
TAAGTATTGT
TTGTGCACTT
GCCGATCTAT
GCGGTGTGAA
ATACCGCACA
GATGCGTAAG
GAGAAAATAC
CGCATCAGGA
AATTGTAAGC
GTTAATATTT
TGTTAAAATT
CGCGTTAAAT
TTTTGTTAAA
TCAGCTCATT
TTTTAACCAA
TAGGCCGAAA
TCGGCAAAAT
CCCTTATAAA
TCAAAAGAAT
AGACCGAGAT
AGGGTTGAGT
GTTGTTCCAG
TTTGGAACAA
GAGTCCACTA
TTAAAGAACG
TGGACTCCAA
CGTCAAAGGG
CGAAAAACCG
TCTATCAGGG
CGATGGCCCA
CTACGTGAAC
CATCACCCTA
ATCAAGTTTT
TTGGGGTCGA
GGTGCCGTAA
AGCACTAAAT
CGGAACCCTA
AAGGGAGCCC
CCGATTTAGA
GCTTGACGGG
GAAAGCCGGC
GAACGTGGCG
AGAAAGGAAG
GGAAGAAAGC
GAAAGGAGCG
GGCGCTAGGG
CGCTGGCAAG
TGTAGCGGTC
ACGCTGCGCG
TAACCACCAC
ACCCGCCGCG
CTTAATGCGC
CGCTACAGGG
CGCGTCCATT
CGCCATTCAG
GCTGCGCAAC
TGTTGGGAAG
GGCGAT