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pGBKT7-TMEM106B(human)

产品货号:P45907

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pGBKT7-TMEM106B(human)
别称: NM_018374.4;HLD16
基因长: 825bp
质粒宿主: 酵母菌
质粒用途: 杂交
片段类型: ORF
片段物种:
原核抗性: Kan
筛选标记: TRP1
荧光标记:
启动子: ADH1
复制子: 2U
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CGGTGCGGGC

CTCTTCGCTA

TTACGCCAGA

TCCTTTTGTT

GTTTCCGGGT

GTACAATATG

61

GACTTCCTCT

TTTCTGGCAA

CCAAACCCAT

ACATCGGGAT

TCCTATAATA

CCTTCGTTGG

121

TCTCCCTAAC

ATGTAGGTGG

CGGAGGGGAG

ATATACAATA

GAACAGATAC

CAGACAAGAC

181

ATAATGGGCT

AAACAAGACT

ACACCAATTA

CACTGCCTCA

TTGATGGTGG

TACATAACGA

241

ACTAATACTG

TAGCCCTAGA

CTTGATAGCC

ATCATCATAT

CGAAGTTTCA

CTACCCTTTT

301

TCCATTTGCC

ATCTATTGAA

GTAATAATAG

GCGCATGCAA

CTTCTTTTCT

TTTTTTTTCT

361

TTTCTCTCTC

CCCCGTTGTT

GTCTCACCAT

ATCCGCAATG

ACAAAAAAAT

GATGGAAGAC

421

ACTAAAGGAA

AAAATTAACG

ACAAAGACAG

CACCAACAGA

TGTCGTTGTT

CCAGAGCTGA

481

TGAGGGGTAT

CTCGAAGCAC

ACGAAACTTT

TTCCTTCCTT

CATTCACGCA

CACTACTCTC

541

TAATGAGCAA

CGGTATACGG

CCTTCCTTCC

AGTTACTTGA

ATTTGAAATA

AAAAAAAGTT

601

TGCTGTCTTG

CTATCAAGTA

TAAATAGACC

TGCAATTATT

AATCTTTTGT

TTCCTCGTCA

661

TTGTTCTCGT

TCCCTTTCTT

CCTTGTTTCT

TTTTCTGCAC

AATATTTCAA

GCTATACCAA

721

GCATACAATC

AACTCCAAGC

TTGAAGCAAG

CCTCCTGAAA

GATGAAGCTA

CTGTCTTCTA

781

TCGAACAAGC

ATGCGATATT

TGCCGACTTA

AAAAGCTCAA

GTGCTCCAAA

GAAAAACCGA

841

AGTGCGCCAA

GTGTCTGAAG

AACAACTGGG

AGTGTCGCTA

CTCTCCCAAA

ACCAAAAGGT

901

CTCCGCTGAC

TAGGGCACAT

CTGACAGAAG

TGGAATCAAG

GCTAGAAAGA

CTGGAACAGC

961

TATTTCTACT

GATTTTTCCT

CGAGAAGACC

TTGACATGAT

TTTGAAAATG

GATTCTTTAC

1021

AGGATATAAA

AGCATTGTTA

ACAGGATTAT

TTGTACAAGA

TAATGTGAAT

AAAGATGCCG

1081

TCACAGATAG

ATTGGCTTCA

GTGGAGACTG

ATATGCCTCT

AACATTGAGA

CAGCATAGAA

1141

TAAGTGCGAC

ATCATCATCG

GAAGAGAGTA

GTAACAAAGG

TCAAAGACAG

TTGACTGTAT

1201

CGCCGGAATT

TGTAATACGA

CTCACTATAG

GGCGAGCCGC

CATCATGGAG

GAGCAGAAGC

1261

TGATCTCAGA

GGAGGACCTG

CATATGGCCA

TGGAGGCCGA

ATTCATGGGA

AAGTCTCTTT

1321

CTCATTTGCC

TTTGCATTCA

AGCAAAGAAG

ATGCTTATGA

TGGAGTCACA

TCTGAAAACA

1381

TGAGGAATGG

ACTGGTTAAT

AGTGAAGTCC

ATAATGAAGA

TGGAAGAAAT

GGAGATGTCT

1441

CTCAGTTTCC

ATATGTGGAA

TTTACAGGAA

GAGATAGTGT

CACCTGCCCT

ACTTGTCAGG

1501

GAACAGGAAG

AATTCCTAGG

GGGCAAGAAA

ACCAACTGGT

GGCATTGATT

CCATATAGTG

1561

ATCAGAGATT

AAGGCCAAGA

AGAACAAAGC

TGTATGTGAT

GGCTTCTGTG

TTTGTCTGTC

1621

TACTCCTTTC

TGGATTGGCT

GTGTTTTTCC

TTTTCCCTCG

CTCTATCGAC

GTGAAATACA

1681

TTGGTGTAAA

ATCAGCCTAT

GTCAGTTATG

ATGTTCAGAA

GCGTACAATT

TATTTAAATA

1741

TCACAAACAC

ACTAAATATA

ACAAACAATA

ACTATTACTC

TGTCGAAGTT

GAAAACATCA

1801

CTGCCCAAGT

TCAATTTTCA

AAAACAGTTA

TTGGAAAGGC

ACGCTTAAAC

AACATAACCA

1861

TTATTGGTCC

ACTTGATATG

AAACAAATTG

ATTACACAGT

ACCTACCGTT

ATAGCAGAGG

1921

AAATGAGTTA

TATGTATGAT

TTCTGTACTC

TGATATCCAT

CAAAGTGCAT

AACATAGTAC

1981

TCATGATGCA

AGTTACTGTG

ACAACAACAT

ACTTTGGCCA

CTCTGAACAG

ATATCCCAGG

2041

AGAGGTATCA

GTATGTCGAC

TGTGGAAGAA

ACACAACTTA

TCAGTTGGGG

CAGTCTGAAT

2101

ATTTAAATGT

ACTTCAGCCA

CAACAGTAAG

GATCCGTCGA

CCTGCAGCGG

CCGCATAACT

2161

AGCATAACCC

CTTGGGGCCT

CTAAACGGGT

CTTGAGGGGT

TTTTTGCGCG

CTTGCAGCCA

2221

AGCTAATTCC

GGGCGAATTT

CTTATGATTT

ATGATTTTTA

TTATTAAATA

AGTTATAAAA

2281

AAAATAAGTG

TATACAAATT

TTAAAGTGAC

TCTTAGGTTT

TAAAACGAAA

ATTCTTATTC

2341

TTGAGTAACT

CTTTCCTGTA

GGTCAGGTTG

CTTTCTCAGG

TATAGCATGA

GGTCGCTCTT

2401

ATTGACCACA

CCTCTACCGG

CATGCAAGCT

TGGCGTAATC

ATGGTCATAG

CTGTTTCCTG

2461

TGTGAAATTG

TTATCCGCTC

ACAATTCCAC

ACAACATACG

AGCCGGAAGC

ATAAAGTGTA

2521

AAGCCTGGGG

TGCCTAATGA

GTGAGCTAAC

TCACATTAAT

TGCGTTGCGC

TCACTGCCCG

2581

CTTTCCAGTC

GGGAAACCTG

TCGTGCCAGC

TGCATTAATG

AATCGGCCAA

CGCGCGGGGA

2641

GAGGCGGTTT

GCGTATTGGG

CGCTCTTCCG

CTTCCTCGCT

CACTGACTCG

CTGCGCTCGG

2701

TCGTTCGGCT

GCGGCGAGCG

GTATCAGCTC

ACTCAAAGGC

GGTAATACGG

TTATCCACAG

2761

AATCAGGGGA

TAACGCAGGA

AAGAACATGT

GAGCAAAAGG

CCAGCAAAAG

GCCAGGAACC

2821

GTAAAAAGGC

CGCGTTGCTG

GCGTTTTTCC

ATAGGCTCCG

CCCCCCTGAC

GAGCATCACA

2881

AAAATCGACG

CTCAAGTCAG

AGGTGGCGAA

ACCCGACAGG

ACTATAAAGA

TACCAGGCGT

2941

TTCCCCCTGG

AAGCTCCCTC

GTGCGCTCTC

CTGTTCCGAC

CCTGCCGCTT

ACCGGATACC

3001

TGTCCGCCTT

TCTCCCTTCG

GGAAGCGTGG

CGCTTTCTCA

TAGCTCACGC

TGTAGGTATC

3061

TCAGTTCGGT

GTAGGTCGTT

CGCTCCAAGC

TGGGCTGTGT

GCACGAACCC

CCCGTTCAGC

3121

CCGACCGCTG

CGCCTTATCC

GGTAACTATC

GTCTTGAGTC

CAACCCGGTA

AGACACGACT

3181

TATCGCCACT

GGCAGCAGCC

ACTGGTAACA

GGATTAGCAG

AGCGAGGTAT

GTAGGCGGTG

3241

CTACAGAGTT

CTTGAAGTGG

TGGCCTAACT

ACGGCTACAC

TAGAAGAACA

GTATTTGGTA

3301

TCTGCGCTCT

GCTGAAGCCA

GTTACCTTCG

GAAAAAGAGT

TGGTAGCTCT

TGATCCGGCA

3361

AACAAACCAC

CGCTGGTAGC

GGTGGTTTTT

TTGTTTGCAA

GCAGCAGATT

ACGCGCAGAA

3421

AAAAAGGATC

TCAAGAAGAT

CCTTTGATCT

TTTCTACGGG

GTCTGACGCT

CAGTGGAACG

3481

AAAACTCACG

TTAAGGGATT

TTGGTCATGA

GATTATCAAA

AAGGATCTTC

ACCTAGATCC

3541

TTTTAAATTA

AAAATGAAGT

TTTAAATCAA

TCTAAAGTAT

ATATGAGTAA

CCTGAGGCTA

3601

TGGCAGGGCC

TGCCGCCCCG

ACGTTGGCTG

CGAGCCCTGG

GCCTTCACCC

GAACTTGGGG

3661

GGTGGGGTGG

GGAAAAGGAA

GAAACGCGGG

CGTATTGGCC

CCAATGGGGT

CTCGGTGGGG

3721

TATCGACAGA

GTGCCAGCCC

TGGGACCGAA

CCCCGCGTTT

ATGAACAAAC

GACCCAACAC

3781

CGTGCGTTTT

ATTCTGTCTT

TTTATTGCCG

TCATAGCGCG

GGTTCCTTCC

GGTATTGTCT

3841

CCTTCCGTGT

TTCAGTTAGC

CTCCCCCTAG

GGTGGGCGAA

GAACTCCAGC

ATGAGATCCC

3901

CGCGCTGGAG

GATCATCCAG

CCGGCGTCCC

GGAAAACGAT

TCCGAAGCCC

AACCTTTCAT

3961

AGAAGGCGGC

GGTGGAATCG

AAATCTCGTG

ATGGCAGGTT

GGGCGTCGCT

TGGTCGGTCA

4021

TTTCGAACCC

CAGAGTCCCG

CTCAGAAGAA

CTCGTCAAGA

AGGCGATAGA

AGGCGATGCG

4081

CTGCGAATCG

GGAGCGGCGA

TACCGTAAAG

CACGAGGAAG

CGGTCAGCCC

ATTCGCCGCC

4141

AAGCTCTTCA

GCAATATCAC

GGGTAGCCAA

CGCTATGTCC

TGATAGCGGT

CCGCCACACC

4201

CAGCCGGCCA

CAGTCGATGA

ATCCAGAAAA

GCGGCCATTT

TCCACCATGA

TATTCGGCAA

4261

GCAGGCATCG

CCATGAGTCA

CGACGAGATC

CTCGCCGTCG

GGCATGCTCG

CCTTGAGCCT

4321

GGCGAACAGT

TCGGCTGGCG

CGAGCCCCTG

ATGCTCTTCG

TCCAGATCAT

CCTGATCGAC

4381

AAGACCGGCT

TCCATCCGAG

TACGTGCTCG

CTCGATGCGA

TGTTTCGCTT

GGTGGTCGAA

4441

TGGGCAGGTA

GCCGGATCAA

GCGTATGCAG

CCGCCGCATT

GCATCAGCCA

TGATGGATAC

4501

TTTCTCGGCA

GGAGCAAGGT

GAGATGACAG

GAGATCCTGC

CCCGGCACTT

CGCCCAATAG

4561

CAGCCAGTCC

CTTCCCGCTT

CAGTGACAAC

GTCGAGCACA

GCTGCGCAAG

GAACGCCCGT

4621

CGTGGCCAGC

CACGATAGCC

GCGCTGCCTC

GTCTTGCAGT

TCATTCAGGG

CACCGGACAG

4681

GTCGGTCTTG

ACAAAAAGAA

CCGGGCGCCC

CTGCGCTGAC

AGCCGGAACA

CGGCGGCATC

4741

AGAGCAGCCG

ATTGTCTGTT

GTGCCCAGTC

ATAGCCGAAT

AGCCTCTCCA

CCCAAGCGGC

4801

CGGAGAACCT

GCGTGCAATC

CATCTTGTTC

AATCATACTC

TTCCTTTTTC

AATATTATTG

4861

AAGCATTTAT

CAGGGTTATT

GTCTCATGAG

CGGATACATA

TTTGAATGTA

TTTAGAAAAA

4921

TAAACAAATA

GGGGTTCCGC

GCACATTTCC

CCGAAAAGTG

CCACCTGAAC

GAAGCATCTG

4981

TGCTTCATTT

TGTAGAACAA

AAATGCAACG

CGAGAGCGCT

AATTTTTCAA

ACAAAGAATC

5041

TGAGCTGCAT

TTTTACAGAA

CAGAAATGCA

ACGCGAAAGC

GCTATTTTAC

CAACGAAGAA

5101

TCTGTGCTTC

ATTTTTGTAA

AACAAAAATG

CAACGCGAGA

GCGCTAATTT

TTCAAACAAA

5161

GAATCTGAGC

TGCATTTTTA

CAGAACAGAA

ATGCAACGCG

AGAGCGCTAT

TTTACCAACA

5221

AAGAATCTAT

ACTTCTTTTT

TGTTCTACAA

AAATGCATCC

CGAGAGCGCT

ATTTTTCTAA

5281

CAAAGCATCT

TAGATTACTT

TTTTTCTCCT

TTGTGCGCTC

TATAATGCAG

TCTCTTGATA

5341

ACTTTTTGCA

CTGTAGGTCC

GTTAAGGTTA

GAAGAAGGCT

ACTTTGGTGT

CTATTTTCTC

5401

TTCCATAAAA

AAAGCCTGAC

TCCACTTCCC

GCGTTTACTG

ATTACTAGCG

AAGCTGCGGG

5461

TGCATTTTTT

CAAGATAAAG

GCATCCCCGA

TTATATTCTA

TACCGATGTG

GATTGCGCAT

5521

ACTTTGTGAA

CAGAAAGTGA

TAGCGTTGAT

GATTCTTCAT

TGGTCAGAAA

ATTATGAACG

5581

GTTTCTTCTA

TTTTGTCTCT

ATATACTACG

TATAGGAAAT

GTTTACATTT

TCGTATTGTT

5641

TTCGATTCAC

TCTATGAATA

GTTCTTACTA

CAATTTTTTT

GTCTAAAGAG

TAATACTAGA

5701

GATAAACATA

AAAAATGTAG

AGGTCGAGTT

TAGATGCAAG

TTCAAGGAGC

GAAAGGTGGA

5761

TGGGTAGGTT

ATATAGGGAT

ATAGCACAGA

GATATATAGC

AAAGAGATAC

TTTTGAGCAA

5821

TGTTTGTGGA

AGCGGTATTC

GCAATATTTT

AGTAGCTCGT

TACAGTCCGG

TGCGTTTTTG

5881

GTTTTTTGAA

AGTGCGTCTT

CAGAGCGCTT

TTGGTTTTCA

AAAGCGCTCT

GAAGTTCCTA

5941

TACTTTCTAG

AGAATAGGAA

CTTCGGAATA

GGAACTTCAA

AGCGTTTCCG

AAAACGAGCG

6001

CTTCCGAAAA

TGCAACGCGA

GCTGCGCACA

TACAGCTCAC

TGTTCACGTC

GCACCTATAT

6061

CTGCGTGTTG

CCTGTATATA

TATATACATG

AGAAGAACGG

CATAGTGCGT

GTTTATGCTT

6121

AAATGCGTAC

TTATATGCGT

CTATTTATGT

AGGATGAAAG

GTAGTCTAGT

ACCTCCTGTG

6181

ATATTATCCC

ATTCCATGCG

GGGTATCGTA

TGCTTCCTTC

AGCACTACCC

TTTAGCTGTT

6241

CTATATGCTG

CCACTCCTCA

ATTGGATTAG

TCTCATCCTT

CAATGCTATC

ATTTCCTTTG

6301

ATATTGGATC

ATACTAAGAA

ACCATTATTA

TCATGACATT

AACCTATAAA

AATAGGCGTA

6361

TCACGAGGCC

CTTTCGTCTC

GCGCGTTTCG

GTGATGACGG

TGAAAACCTC

TGACACATGC

6421

AGCTCCCGGA

GACGGTCACA

GCTTGTCTGT

AAGCGGATGC

CGGGAGCAGA

CAAGCCCGTC

6481

AGGGCGCGTC

AGCGGGTGTT

GGCGGGTGTC

GGGGCTGGCT

TAACTATGCG

GCATCAGAGC

6541

AGATTGTACT

GAGAGTGCAC

CATAGATCAA

CGACATTACT

ATATATATAA

TATAGGAAGC

6601

ATTTAATAGA

ACAGCATCGT

AATATATGTG

TACTTTGCAG

TTATGACGCC

AGATGGCAGT

6661

AGTGGAAGAT

ATTCTTTATT

GAAAAATAGC

TTGTCACCTT

ACGTACAATC

TTGATCCGGA

6721

GCTTTTCTTT

TTTTGCCGAT

TAAGAATTAA

TTCGGTCGAA

AAAAGAAAAG

GAGAGGGCCA

6781

AGAGGGAGGG

CATTGGTGAC

TATTGAGCAC

GTGAGTATAC

GTGATTAAGC

ACACAAAGGC

6841

AGCTTGGAGT

ATGTCTGTTA

TTAATTTCAC

AGGTAGTTCT

GGTCCATTGG

TGAAAGTTTG

6901

CGGCTTGCAG

AGCACAGAGG

CCGCAGAATG

TGCTCTAGAT

TCCGATGCTG

ACTTGCTGGG

6961

TATTATATGT

GTGCCCAATA

GAAAGAGAAC

AATTGACCCG

GTTATTGCAA

GGAAAATTTC

7021

AAGTCTTGTA

AAAGCATATA

AAAATAGTTC

AGGCACTCCG

AAATACTTGG

TTGGCGTGTT

7081

TCGTAATCAA

CCTAAGGAGG

ATGTTTTGGC

TCTGGTCAAT

GATTACGGCA

TTGATATCGT

7141

CCAACTGCAT

GGAGATGAGT

CGTGGCAAGA

ATACCAAGAG

TTCCTCGGTT

TGCCAGTTAT

7201

TAAAAGACTC

GTATTTCCAA

AAGACTGCAA

CATACTACTC

AGTGCAGCTT

CACAGAAACC

7261

TCATTCGTTT

ATTCCCTTGT

TTGATTCAGA

AGCAGGTGGG

ACAGGTGAAC

TTTTGGATTG

7321

GAACTCGATT

TCTGACTGGG

TTGGAAGGCA

AGAGAGCCCC

GAAAGCTTAC

ATTTTATGTT

7381

AGCTGGTGGA

CTGACGCCAG

AAAATGTTGG

TGATGCGCTT

AGATTAAATG

GCGTTATTGG

7441

TGTTGATGTA

AGCGGAGGTG

TGGAGACAAA

TGGTGTAAAA

GACTCTAACA

AAATAGCAAA

7501

TTTCGTCAAA

AATGCTAAGA

AATAGGTTAT

TACTGAGTAG

TATTTATTTA

AGTATTGTTT

7561

GTGCACTTGC

CGATCTATGC

GGTGTGAAAT

ACCGCACAGA

TGCGTAAGGA

GAAAATACCG

7621

CATCAGGAAA

TTGTAAGCGT

TAATATTTTG

TTAAAATTCG

CGTTAAATTT

TTGTTAAATC

7681

AGCTCATTTT

TTAACCAATA

GGCCGAAATC

GGCAAAATCC

CTTATAAATC

AAAAGAATAG

7741

ACCGAGATAG

GGTTGAGTGT

TGTTCCAGTT

TGGAACAAGA

GTCCACTATT

AAAGAACGTG

7801

GACTCCAACG

TCAAAGGGCG

AAAAACCGTC

TATCAGGGCG

ATGGCCCACT

ACGTGAACCA

7861

TCACCCTAAT

CAAGTTTTTT

GGGGTCGAGG

TGCCGTAAAG

CACTAAATCG

GAACCCTAAA

7921

GGGAGCCCCC

GATTTAGAGC

TTGACGGGGA

AAGCCGGCGA

ACGTGGCGAG

AAAGGAAGGG

7981

AAGAAAGCGA

AAGGAGCGGG

CGCTAGGGCG

CTGGCAAGTG

TAGCGGTCAC

GCTGCGCGTA

8041

ACCACCACAC

CCGCCGCGCT

TAATGCGCCG

CTACAGGGCG

CGTCCATTCG

CCATTCAGGC

8101

TGCGCAACTG

TTGGGAAGGG

CGAT

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