名称: | pGBKT7-TMEM106B(human) |
---|---|
别称: | NM_018374.4;HLD16 |
基因长: | 825bp |
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Kan |
筛选标记: | TRP1 |
荧光标记: | |
启动子: | ADH1 |
复制子: | 2U |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGGTGCGGGC
CTCTTCGCTA
TTACGCCAGA
TCCTTTTGTT
GTTTCCGGGT
GTACAATATG
GACTTCCTCT
TTTCTGGCAA
CCAAACCCAT
ACATCGGGAT
TCCTATAATA
CCTTCGTTGG
TCTCCCTAAC
ATGTAGGTGG
CGGAGGGGAG
ATATACAATA
GAACAGATAC
CAGACAAGAC
ATAATGGGCT
AAACAAGACT
ACACCAATTA
CACTGCCTCA
TTGATGGTGG
TACATAACGA
ACTAATACTG
TAGCCCTAGA
CTTGATAGCC
ATCATCATAT
CGAAGTTTCA
CTACCCTTTT
TCCATTTGCC
ATCTATTGAA
GTAATAATAG
GCGCATGCAA
CTTCTTTTCT
TTTTTTTTCT
TTTCTCTCTC
CCCCGTTGTT
GTCTCACCAT
ATCCGCAATG
ACAAAAAAAT
GATGGAAGAC
ACTAAAGGAA
AAAATTAACG
ACAAAGACAG
CACCAACAGA
TGTCGTTGTT
CCAGAGCTGA
TGAGGGGTAT
CTCGAAGCAC
ACGAAACTTT
TTCCTTCCTT
CATTCACGCA
CACTACTCTC
TAATGAGCAA
CGGTATACGG
CCTTCCTTCC
AGTTACTTGA
ATTTGAAATA
AAAAAAAGTT
TGCTGTCTTG
CTATCAAGTA
TAAATAGACC
TGCAATTATT
AATCTTTTGT
TTCCTCGTCA
TTGTTCTCGT
TCCCTTTCTT
CCTTGTTTCT
TTTTCTGCAC
AATATTTCAA
GCTATACCAA
GCATACAATC
AACTCCAAGC
TTGAAGCAAG
CCTCCTGAAA
GATGAAGCTA
CTGTCTTCTA
TCGAACAAGC
ATGCGATATT
TGCCGACTTA
AAAAGCTCAA
GTGCTCCAAA
GAAAAACCGA
AGTGCGCCAA
GTGTCTGAAG
AACAACTGGG
AGTGTCGCTA
CTCTCCCAAA
ACCAAAAGGT
CTCCGCTGAC
TAGGGCACAT
CTGACAGAAG
TGGAATCAAG
GCTAGAAAGA
CTGGAACAGC
TATTTCTACT
GATTTTTCCT
CGAGAAGACC
TTGACATGAT
TTTGAAAATG
GATTCTTTAC
AGGATATAAA
AGCATTGTTA
ACAGGATTAT
TTGTACAAGA
TAATGTGAAT
AAAGATGCCG
TCACAGATAG
ATTGGCTTCA
GTGGAGACTG
ATATGCCTCT
AACATTGAGA
CAGCATAGAA
TAAGTGCGAC
ATCATCATCG
GAAGAGAGTA
GTAACAAAGG
TCAAAGACAG
TTGACTGTAT
CGCCGGAATT
TGTAATACGA
CTCACTATAG
GGCGAGCCGC
CATCATGGAG
GAGCAGAAGC
TGATCTCAGA
GGAGGACCTG
CATATGGCCA
TGGAGGCCGA
ATTCATGGGA
AAGTCTCTTT
CTCATTTGCC
TTTGCATTCA
AGCAAAGAAG
ATGCTTATGA
TGGAGTCACA
TCTGAAAACA
TGAGGAATGG
ACTGGTTAAT
AGTGAAGTCC
ATAATGAAGA
TGGAAGAAAT
GGAGATGTCT
CTCAGTTTCC
ATATGTGGAA
TTTACAGGAA
GAGATAGTGT
CACCTGCCCT
ACTTGTCAGG
GAACAGGAAG
AATTCCTAGG
GGGCAAGAAA
ACCAACTGGT
GGCATTGATT
CCATATAGTG
ATCAGAGATT
AAGGCCAAGA
AGAACAAAGC
TGTATGTGAT
GGCTTCTGTG
TTTGTCTGTC
TACTCCTTTC
TGGATTGGCT
GTGTTTTTCC
TTTTCCCTCG
CTCTATCGAC
GTGAAATACA
TTGGTGTAAA
ATCAGCCTAT
GTCAGTTATG
ATGTTCAGAA
GCGTACAATT
TATTTAAATA
TCACAAACAC
ACTAAATATA
ACAAACAATA
ACTATTACTC
TGTCGAAGTT
GAAAACATCA
CTGCCCAAGT
TCAATTTTCA
AAAACAGTTA
TTGGAAAGGC
ACGCTTAAAC
AACATAACCA
TTATTGGTCC
ACTTGATATG
AAACAAATTG
ATTACACAGT
ACCTACCGTT
ATAGCAGAGG
AAATGAGTTA
TATGTATGAT
TTCTGTACTC
TGATATCCAT
CAAAGTGCAT
AACATAGTAC
TCATGATGCA
AGTTACTGTG
ACAACAACAT
ACTTTGGCCA
CTCTGAACAG
ATATCCCAGG
AGAGGTATCA
GTATGTCGAC
TGTGGAAGAA
ACACAACTTA
TCAGTTGGGG
CAGTCTGAAT
ATTTAAATGT
ACTTCAGCCA
CAACAGTAAG
GATCCGTCGA
CCTGCAGCGG
CCGCATAACT
AGCATAACCC
CTTGGGGCCT
CTAAACGGGT
CTTGAGGGGT
TTTTTGCGCG
CTTGCAGCCA
AGCTAATTCC
GGGCGAATTT
CTTATGATTT
ATGATTTTTA
TTATTAAATA
AGTTATAAAA
AAAATAAGTG
TATACAAATT
TTAAAGTGAC
TCTTAGGTTT
TAAAACGAAA
ATTCTTATTC
TTGAGTAACT
CTTTCCTGTA
GGTCAGGTTG
CTTTCTCAGG
TATAGCATGA
GGTCGCTCTT
ATTGACCACA
CCTCTACCGG
CATGCAAGCT
TGGCGTAATC
ATGGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGAACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
CCTGAGGCTA
TGGCAGGGCC
TGCCGCCCCG
ACGTTGGCTG
CGAGCCCTGG
GCCTTCACCC
GAACTTGGGG
GGTGGGGTGG
GGAAAAGGAA
GAAACGCGGG
CGTATTGGCC
CCAATGGGGT
CTCGGTGGGG
TATCGACAGA
GTGCCAGCCC
TGGGACCGAA
CCCCGCGTTT
ATGAACAAAC
GACCCAACAC
CGTGCGTTTT
ATTCTGTCTT
TTTATTGCCG
TCATAGCGCG
GGTTCCTTCC
GGTATTGTCT
CCTTCCGTGT
TTCAGTTAGC
CTCCCCCTAG
GGTGGGCGAA
GAACTCCAGC
ATGAGATCCC
CGCGCTGGAG
GATCATCCAG
CCGGCGTCCC
GGAAAACGAT
TCCGAAGCCC
AACCTTTCAT
AGAAGGCGGC
GGTGGAATCG
AAATCTCGTG
ATGGCAGGTT
GGGCGTCGCT
TGGTCGGTCA
TTTCGAACCC
CAGAGTCCCG
CTCAGAAGAA
CTCGTCAAGA
AGGCGATAGA
AGGCGATGCG
CTGCGAATCG
GGAGCGGCGA
TACCGTAAAG
CACGAGGAAG
CGGTCAGCCC
ATTCGCCGCC
AAGCTCTTCA
GCAATATCAC
GGGTAGCCAA
CGCTATGTCC
TGATAGCGGT
CCGCCACACC
CAGCCGGCCA
CAGTCGATGA
ATCCAGAAAA
GCGGCCATTT
TCCACCATGA
TATTCGGCAA
GCAGGCATCG
CCATGAGTCA
CGACGAGATC
CTCGCCGTCG
GGCATGCTCG
CCTTGAGCCT
GGCGAACAGT
TCGGCTGGCG
CGAGCCCCTG
ATGCTCTTCG
TCCAGATCAT
CCTGATCGAC
AAGACCGGCT
TCCATCCGAG
TACGTGCTCG
CTCGATGCGA
TGTTTCGCTT
GGTGGTCGAA
TGGGCAGGTA
GCCGGATCAA
GCGTATGCAG
CCGCCGCATT
GCATCAGCCA
TGATGGATAC
TTTCTCGGCA
GGAGCAAGGT
GAGATGACAG
GAGATCCTGC
CCCGGCACTT
CGCCCAATAG
CAGCCAGTCC
CTTCCCGCTT
CAGTGACAAC
GTCGAGCACA
GCTGCGCAAG
GAACGCCCGT
CGTGGCCAGC
CACGATAGCC
GCGCTGCCTC
GTCTTGCAGT
TCATTCAGGG
CACCGGACAG
GTCGGTCTTG
ACAAAAAGAA
CCGGGCGCCC
CTGCGCTGAC
AGCCGGAACA
CGGCGGCATC
AGAGCAGCCG
ATTGTCTGTT
GTGCCCAGTC
ATAGCCGAAT
AGCCTCTCCA
CCCAAGCGGC
CGGAGAACCT
GCGTGCAATC
CATCTTGTTC
AATCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGAAC
GAAGCATCTG
TGCTTCATTT
TGTAGAACAA
AAATGCAACG
CGAGAGCGCT
AATTTTTCAA
ACAAAGAATC
TGAGCTGCAT
TTTTACAGAA
CAGAAATGCA
ACGCGAAAGC
GCTATTTTAC
CAACGAAGAA
TCTGTGCTTC
ATTTTTGTAA
AACAAAAATG
CAACGCGAGA
GCGCTAATTT
TTCAAACAAA
GAATCTGAGC
TGCATTTTTA
CAGAACAGAA
ATGCAACGCG
AGAGCGCTAT
TTTACCAACA
AAGAATCTAT
ACTTCTTTTT
TGTTCTACAA
AAATGCATCC
CGAGAGCGCT
ATTTTTCTAA
CAAAGCATCT
TAGATTACTT
TTTTTCTCCT
TTGTGCGCTC
TATAATGCAG
TCTCTTGATA
ACTTTTTGCA
CTGTAGGTCC
GTTAAGGTTA
GAAGAAGGCT
ACTTTGGTGT
CTATTTTCTC
TTCCATAAAA
AAAGCCTGAC
TCCACTTCCC
GCGTTTACTG
ATTACTAGCG
AAGCTGCGGG
TGCATTTTTT
CAAGATAAAG
GCATCCCCGA
TTATATTCTA
TACCGATGTG
GATTGCGCAT
ACTTTGTGAA
CAGAAAGTGA
TAGCGTTGAT
GATTCTTCAT
TGGTCAGAAA
ATTATGAACG
GTTTCTTCTA
TTTTGTCTCT
ATATACTACG
TATAGGAAAT
GTTTACATTT
TCGTATTGTT
TTCGATTCAC
TCTATGAATA
GTTCTTACTA
CAATTTTTTT
GTCTAAAGAG
TAATACTAGA
GATAAACATA
AAAAATGTAG
AGGTCGAGTT
TAGATGCAAG
TTCAAGGAGC
GAAAGGTGGA
TGGGTAGGTT
ATATAGGGAT
ATAGCACAGA
GATATATAGC
AAAGAGATAC
TTTTGAGCAA
TGTTTGTGGA
AGCGGTATTC
GCAATATTTT
AGTAGCTCGT
TACAGTCCGG
TGCGTTTTTG
GTTTTTTGAA
AGTGCGTCTT
CAGAGCGCTT
TTGGTTTTCA
AAAGCGCTCT
GAAGTTCCTA
TACTTTCTAG
AGAATAGGAA
CTTCGGAATA
GGAACTTCAA
AGCGTTTCCG
AAAACGAGCG
CTTCCGAAAA
TGCAACGCGA
GCTGCGCACA
TACAGCTCAC
TGTTCACGTC
GCACCTATAT
CTGCGTGTTG
CCTGTATATA
TATATACATG
AGAAGAACGG
CATAGTGCGT
GTTTATGCTT
AAATGCGTAC
TTATATGCGT
CTATTTATGT
AGGATGAAAG
GTAGTCTAGT
ACCTCCTGTG
ATATTATCCC
ATTCCATGCG
GGGTATCGTA
TGCTTCCTTC
AGCACTACCC
TTTAGCTGTT
CTATATGCTG
CCACTCCTCA
ATTGGATTAG
TCTCATCCTT
CAATGCTATC
ATTTCCTTTG
ATATTGGATC
ATACTAAGAA
ACCATTATTA
TCATGACATT
AACCTATAAA
AATAGGCGTA
TCACGAGGCC
CTTTCGTCTC
GCGCGTTTCG
GTGATGACGG
TGAAAACCTC
TGACACATGC
AGCTCCCGGA
GACGGTCACA
GCTTGTCTGT
AAGCGGATGC
CGGGAGCAGA
CAAGCCCGTC
AGGGCGCGTC
AGCGGGTGTT
GGCGGGTGTC
GGGGCTGGCT
TAACTATGCG
GCATCAGAGC
AGATTGTACT
GAGAGTGCAC
CATAGATCAA
CGACATTACT
ATATATATAA
TATAGGAAGC
ATTTAATAGA
ACAGCATCGT
AATATATGTG
TACTTTGCAG
TTATGACGCC
AGATGGCAGT
AGTGGAAGAT
ATTCTTTATT
GAAAAATAGC
TTGTCACCTT
ACGTACAATC
TTGATCCGGA
GCTTTTCTTT
TTTTGCCGAT
TAAGAATTAA
TTCGGTCGAA
AAAAGAAAAG
GAGAGGGCCA
AGAGGGAGGG
CATTGGTGAC
TATTGAGCAC
GTGAGTATAC
GTGATTAAGC
ACACAAAGGC
AGCTTGGAGT
ATGTCTGTTA
TTAATTTCAC
AGGTAGTTCT
GGTCCATTGG
TGAAAGTTTG
CGGCTTGCAG
AGCACAGAGG
CCGCAGAATG
TGCTCTAGAT
TCCGATGCTG
ACTTGCTGGG
TATTATATGT
GTGCCCAATA
GAAAGAGAAC
AATTGACCCG
GTTATTGCAA
GGAAAATTTC
AAGTCTTGTA
AAAGCATATA
AAAATAGTTC
AGGCACTCCG
AAATACTTGG
TTGGCGTGTT
TCGTAATCAA
CCTAAGGAGG
ATGTTTTGGC
TCTGGTCAAT
GATTACGGCA
TTGATATCGT
CCAACTGCAT
GGAGATGAGT
CGTGGCAAGA
ATACCAAGAG
TTCCTCGGTT
TGCCAGTTAT
TAAAAGACTC
GTATTTCCAA
AAGACTGCAA
CATACTACTC
AGTGCAGCTT
CACAGAAACC
TCATTCGTTT
ATTCCCTTGT
TTGATTCAGA
AGCAGGTGGG
ACAGGTGAAC
TTTTGGATTG
GAACTCGATT
TCTGACTGGG
TTGGAAGGCA
AGAGAGCCCC
GAAAGCTTAC
ATTTTATGTT
AGCTGGTGGA
CTGACGCCAG
AAAATGTTGG
TGATGCGCTT
AGATTAAATG
GCGTTATTGG
TGTTGATGTA
AGCGGAGGTG
TGGAGACAAA
TGGTGTAAAA
GACTCTAACA
AAATAGCAAA
TTTCGTCAAA
AATGCTAAGA
AATAGGTTAT
TACTGAGTAG
TATTTATTTA
AGTATTGTTT
GTGCACTTGC
CGATCTATGC
GGTGTGAAAT
ACCGCACAGA
TGCGTAAGGA
GAAAATACCG
CATCAGGAAA
TTGTAAGCGT
TAATATTTTG
TTAAAATTCG
CGTTAAATTT
TTGTTAAATC
AGCTCATTTT
TTAACCAATA
GGCCGAAATC
GGCAAAATCC
CTTATAAATC
AAAAGAATAG
ACCGAGATAG
GGTTGAGTGT
TGTTCCAGTT
TGGAACAAGA
GTCCACTATT
AAAGAACGTG
GACTCCAACG
TCAAAGGGCG
AAAAACCGTC
TATCAGGGCG
ATGGCCCACT
ACGTGAACCA
TCACCCTAAT
CAAGTTTTTT
GGGGTCGAGG
TGCCGTAAAG
CACTAAATCG
GAACCCTAAA
GGGAGCCCCC
GATTTAGAGC
TTGACGGGGA
AAGCCGGCGA
ACGTGGCGAG
AAAGGAAGGG
AAGAAAGCGA
AAGGAGCGGG
CGCTAGGGCG
CTGGCAAGTG
TAGCGGTCAC
GCTGCGCGTA
ACCACCACAC
CCGCCGCGCT
TAATGCGCCG
CTACAGGGCG
CGTCCATTCG
CCATTCAGGC
TGCGCAACTG
TTGGGAAGGG
CGAT