名称: | pRECA(ecoli)-Fluc-SV40-hRluc-Neo |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 启动子检测 |
片段类型: | Promoter |
片段物种: | 大肠杆菌 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | Fluc,Rluc |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CATGCAAGCT
GATCCGGCTG
CTAACAAAGC
CCGAAAGGAA
GCTGAGTTGG
CTGCTGCCAC
CGCTGAGCAA
TAACTAGCAT
AACCCCTTGG
GGCGGCCGCT
TCGAGCAGAC
ATGATAAGAT
ACATTGATGA
GTTTGGACAA
ACCACAACTA
GAATGCAGTG
AAAAAAATGC
TTTATTTGTG
AAATTTGTGA
TGCTATTGCT
TTATTTGTAA
CCATTATAAG
CTGCAATAAA
CAAGTTAACA
ACAACAATTG
CATTCATTTT
ATGTTTCAGG
TTCAGGGGGA
GATGTGGGAG
GTTTTTTTAA
GCAAGTAAAA
CCTCTACAAA
TGTGGTAAAA
TCGAATTTTA
ACAAAATATT
AACGCTTACA
ATTTCCTGAT
GCGGTATTTT
CTCCTTACGC
ATCTGTGCGG
TATTTCACAC
CGCATACGCG
GATCTGCGCA
GCACCATGGC
CTGAAATAAC
CTCTGAAAGA
GGAACTTGGT
TAGGTACCTT
CTGAGGCGGA
AAGAACCAGC
TGTGGAATGT
GTGTCAGTTA
GGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCAGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCATAGTC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGCT
TTTGCAAAAA
GCTTGATTCT
TCTGACACAA
CAGTCTCGAA
CCAAAGGCTG
GAGCCACCAT
GGCTTCCAAG
GTGTACGACC
CCGAGCAACG
CAAACGCATG
ATCACTGGGC
CTCAGTGGTG
GGCTCGCTGC
AAGCAAATGA
ACGTGCTGGA
CTCCTTCATC
AACTACTATG
ATTCCGAGAA
GCACGCCGAG
AACGCCGTGA
TTTTTCTGCA
TGGTAACGCT
GCCTCCAGCT
ACCTGTGGAG
GCACGTCGTG
CCTCACATCG
AGCCCGTGGC
TAGATGCATC
ATCCCTGATC
TGATCGGAAT
GGGTAAGTCC
GGCAAGAGCG
GGAATGGCTC
ATATCGCCTC
CTGGATCACT
ACAAGTACCT
CACCGCTTGG
TTCGAGCTGC
TGAACCTTCC
AAAGAAAATC
ATCTTTGTGG
GCCACGACTG
GGGGGCTTGT
CTGGCCTTTC
ACTACTCCTA
CGAGCACCAA
GACAAGATCA
AGGCCATCGT
CCATGCTGAG
AGTGTCGTGG
ACGTGATCGA
GTCCTGGGAC
GAGTGGCCTG
ACATCGAGGA
GGATATCGCC
CTGATCAAGA
GCGAAGAGGG
CGAGAAAATG
GTGCTTGAGA
ATAACTTCTT
CGTCGAGACC
ATGCTCCCAA
GCAAGATCAT
GCGGAAACTG
GAGCCTGAGG
AGTTCGCTGC
CTACCTGGAG
CCATTCAAGG
AGAAGGGCGA
GGTTAGACGG
CCTACCCTCT
CCTGGCCTCG
CGAGATCCCT
CTCGTTAAGG
GAGGCAAGCC
CGACGTCGTC
CAGATTGTCC
GCAACTACAA
CGCCTACCTT
CGGGCCAGCG
ACGATCTGCC
TAAGATGTTC
ATCGAGTCCG
ACCCTGGGTT
CTTTTCCAAC
GCTATTGTCG
AGGGAGCTAA
GAAGTTCCCT
AACACCGAGT
TCGTGAAGGT
GAAGGGCCTC
CACTTCAGCC
AGGAGGACGC
TCCAGATGAA
ATGGGTAAGT
ACATCAAGAG
CTTCGTGGAG
CGCGTGCTGA
AGAACGAGCA
GACCGGTGGT
GGGAGCGGAG
GTGGCGGATC
AGGTGGCGGA
GGCTCCGGAG
GGATTGAACA
AGATGGATTG
CACGCAGGTT
CTCCGGCCGC
TTGGGTGGAG
AGGCTATTCG
GCTATGACTG
GGCACAACAG
ACAATCGGCT
GCTCTGATGC
CGCCGTGTTC
CGGCTGTCAG
CGCAGGGGCG
CCCGGTTCTT
TTTGTCAAGA
CCGACCTGTC
CGGTGCCCTG
AATGAACTGC
AGGACGAGGC
AGCGCGGCTA
TCGTGGCTGG
CCACGACGGG
CGTTCCTTGC
GCAGCTGTGC
TCGACGTTGT
CACTGAAGCG
GGAAGGGACT
GGCTGCTATT
GGGCGAAGTG
CCGGGGCAGG
ATCTCCTGTC
ATCTCACCTT
GCTCCTGCCG
AGAAAGTATC
CATCATGGCT
GATGCAATGC
GGCGGCTGCA
TACGCTTGAT
CCGGCTACCT
GCCCATTCGA
CCACCAAGCG
AAACATCGCA
TCGAGCGAGC
ACGTACTCGG
ATGGAAGCCG
GTCTTGTCGA
TCAGGATGAT
CTGGACGAAG
AGCATCAGGG
GCTCGCGCCA
GCCGAACTGT
TCGCCAGGCT
CAAGGCGCGC
ATGCCCGACG
GCGAGGATCT
CGTCGTGACC
CATGGCGATG
CCTGCTTGCC
GAATATCATG
GTGGAAAATG
GCCGCTTTTC
TGGATTCATC
GACTGTGGCC
GGCTGGGTGT
GGCGGACCGC
TATCAGGACA
TAGCGTTGGC
TACCCGTGAT
ATTGCTGAAG
AGCTTGGCGG
CGAATGGGCT
GACCGCTTCC
TCGTGCTTTA
CGGTATCGCC
GCTCCCGATT
CGCAGCGCAT
CGCCTTCTAT
CGCCTTCTTG
ACGAGTTCTT
CTGAGCGGGA
CTCTGGGGTT
CGAAATGACC
GACCAAGCGA
CGCCCAACCT
GCCATCACGA
TGGCCGCAAT
AAAATATCTT
TATTTTCATT
ACATCTGTGT
GTTGGTTTTT
TGTGTGAATC
GATAGCGATA
AGGATCCTCT
TTGCGCTTGC
GTTTTCCCTT
GTCCAGATAG
CCCAGTAGCT
GACATTCATC
CGGGGTCAGC
ACCGTTTCTG
CGGACTGGCT
TTCTACGTAA
TGGTTTCTTA
GACGTCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTCAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTA
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACTATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAATTC
GAAATGACCG
ACCAAGCGAC
GCCCAACCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGGACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATTT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTATAGTCC
GGAAATACAG
GAACGCACGC
TGGATGGCCC
TTCGCTGGGA
TGGTGAAACC
ATGAAAAATG
GCAGCTTCAG
TGGATTAAGT
GGGGGTAATG
TGGCCTGTAC
CCTCTGGTTG
CATAGGTATT
CATACGGTTA
AAATTTATCA
GGCGCGATTG
CGGCAGTTTT
TCGGGTGGTT
TGTTGCCATT
TTTACCTGTC
TGCTGCCGTG
ATCGCGCTGA
ACGCGTTTTA
GCGGTGCGTA
CAATTAAGGG
ATTATGGTAA
ATCCACTTAC
TGTCTGCCCT
CGTAGCCATC
GAGATAAACC
GCAGTACTCC
GGCCACGATG
CGTCCGGCGT
AGAGGATCGA
GATCTAGATT
TAAAAGTGTT
ATTTTGATCG
CAAAATGAAA
GATAAATATT
TTATTTTGAA
AGTTTATATA
AAGAGGGTAG
TGAGGAGAAT
GAGTTGAGTT
ATGTAAAGTC
CGTATCGGGC
AGTGACTACC
GCTTCCTTGT
GCGGGGCGGG
ATGCTTTACC
ATACTTGCCC
CTGGTTGAAT
CTGTTAAATG
GACCCCTCAT
GTTCAAGCGT
CGTTATGTAA
CATTGCTTCC
CCTTTTTATG
TTGCTTGCCG
CCTGTAGCAG
CAAGCCAAAA
CCTACTGAGA
CTGAAACGAC
CACCGGAACG
CCGTCTGGCG
GCTTCCTGCT
TGAGCCGCAG
CACAATGTGA
TGCAGATGGG
CGGTGACTTC
GCCAATAACC
CGAATGCCCA
GCAGTTCATC
GACAAAATGG
TGAACAAACA
CGGTTTCGAT
CGTCAGCAGT
TGCAGGAAAT
TCTCTCCCAG
GCGAAACGTC
TGGATTCGGT
ACTGCGGCTG
ATGGATAACC
AGGCACCAAC
CACATCGGTG
AAACCGCCAT
CAGGTCCGAA
CGGCGCATGG
CTGCGTTATC
GCAAAAAATT
TATTACGCCG
GACAACGTGC
AGAACGGTGT
GGTTTTCTGG
AATCAGTATG
AAGATGCGTT
GAATCGCGCG
TGGCAGGTGT
ATGGTGTACC
GCCGGAAATT
ATCGTCGGGA
TTATCGGCGT
TGAAACCCGC
TGGGGGCGCG
TGATGGGGAA
AACTCGCATC
CTCGATGCGC
TGGCAACGCT
GTCATTTAAC
TACCCACGCC
GCGCGGAGTA
TTTTTCTGGC
GAGCTGGAAA
CCTTCCTGCT
GATGGCGCGC
GACGAGCAGG
ACGATCCGCT
CAATCTGAAA
GGTTCCTTTG
CCGGGGCGAT
GGGCTACGGT
CAGTTTATGC
CGTCGTCTTA
CAAACAATAT
GCGGTAGATT
TCAGCGGCGA
CGGGCATATC
AACCTGTGGG
ATCCGGTTGA
TGCGATCGGT
AGCGTGGCGA
ATTATTTCAA
AGCGCACGGC
TGGGTGAAAG
GCGATCAGGT
CGCGGTAATG
GCAAACGGTC
AGGCGCCAGG
CTTGCCAAAT
GGCTTCAAAA
CCAGGTACAG
CATTTCGCAG
CTTGCCGCGG
CAGGTTTAAC
GCCACAGCAG
CCGCTGGGCA
ACCATCAACA
AGCCAGCCTG
CTGCGTCTGG
ATGTTGGCAC
CGGCTACCAG
TACTGGTACG
GTCTGCCGAA
CTTCTACACC
ATTACCCGTT
ATAACCACAG
CACCCATTAC
GCAATGGCGG
TCTGGCAGTT
AGGACAAGCC
GTGGCGCTGG
CGCGCGTGCA
GTAACTATTT
GCAAGAGGGG
GGCGTGAGCT
TCCCTCTGGC
TAATCAGCTT
TTTTCTGAAT
CCTCCTCGTA
AAATTGCAAC
GCCAACACCA
TCTTCCTGAC
GAAAGTGTTA
TCTTGTCCGG
CATAAATTTT
TGACTGACAG
AGGTTGTGAT
GACTGACAGT
GAACTGATGC
AGTTAAGCGA
ACAGGTTGGG
CAGGCGCTGA
AAGCCCGTGG
CGCAACCGTA
ACAACTGCTG
AGTCTTGTAC
CGGCGGTTGG
GTAGCGAAAG
TGATTACCGA
TATTGCCGGC
AGTTCCGCCT
GGTTTGAACG
CGGATTTGTC
ACCTACAGTA
ACGAAGCAAA
AGCGCAAATG
ATCGGTGTAC
GCGAAGAGAC
GCTGGCGCAG
CATGGCGCGG
TGAGCGAACC
TGTCGTGGTG
GAAATGGCGA
TAGGCGCACT
GAAAGCGGCT
CGTGCTGATT
ACGCCGTGTC
TATTAGTGGT
ATCGCCGGGC
CGGATGGCGG
CAGTGAAGAG
AAGCCTGTCG
GCACCGTCTG
GTTTGCTTTT
GCCACTGCCC
GTGGTGAAGG
CATTACCCGG
CGGGAATGCT
TCAGCGGTGA
CCGTGATGCG
GTGCGTCGTC
AGGCTACTGC
GTATGCATTG
CAGACCTTGT
GGCAACAATT
TCTACAAAAC
ACTTGATACT
GTATGAGCAT
ACAGTATAAT
TGCTTCAACA
GAACATATTG
ACTATCCGGT
ATTACCCGGC
ATGACAGGAG
TAAAAGAATT
CTGCAGTCGA
CGGTACCGCG
GGCCCGGGAT
CCCACCATGG
AAGATGCCAA
AAACATTAAG
AAGGGCCCAG
CGCCATTCTA
CCCACTCGAA
GACGGGACCG
CCGGCGAGCA
GCTGCACAAA
GCCATGAAGC
GCTACGCCCT
GGTGCCCGGC
ACCATCGCCT
TTACCGACGC
ACATATCGAG
GTGGACATTA
CCTACGCCGA
GTACTTCGAG
ATGAGCGTTC
GGCTGGCAGA
AGCTATGAAG
CGCTATGGGC
TGAATACAAA
CCATCGGATC
GTGGTGTGCA
GCGAGAATAG
CTTGCAGTTC
TTCATGCCCG
TGTTGGGTGC
CCTGTTCATC
GGTGTGGCTG
TGGCCCCAGC
TAACGACATC
TACAACGAGC
GCGAGCTGCT
GAACAGCATG
GGCATCAGCC
AGCCCACCGT
CGTATTCGTG
AGCAAGAAAG
GGCTGCAAAA
GATCCTCAAC
GTGCAAAAGA
AGCTACCGAT
CATACAAAAG
ATCATCATCA
TGGATAGCAA
GACCGACTAC
CAGGGCTTCC
AAAGCATGTA
CACCTTCGTG
ACTTCCCATT
TGCCACCCGG
CTTCAACGAG
TACGACTTCG
TGCCCGAGAG
CTTCGACCGG
GACAAAACCA
TCGCCCTGAT
CATGAACAGT
AGTGGCAGTA
CCGGATTGCC
CAAGGGCGTA
GCCCTACCGC
ACCGCACCGC
TTGTGTCCGA
TTCAGTCATG
CCCGCGACCC
CATCTTCGGC
AACCAGATCA
TCCCCGACAC
CGCTATCCTC
AGCGTGGTGC
CATTTCACCA
CGGCTTCGGC
ATGTTCACCA
CGCTGGGCTA
CTTGATCTGC
GGCTTTCGGG
TCGTGCTCAT
GTACCGCTTC
GAGGAGGAGC
TATTCTTGCG
CAGCTTGCAA
GACTATAAGA
TTCAATCTGC
CCTGCTGGTG
CCCACACTAT
TTAGCTTCTT
CGCTAAGAGC
ACTCTCATCG
ACAAGTACGA
CCTAAGCAAC
TTGCACGAGA
TCGCCAGCGG
CGGGGCGCCG
CTCAGCAAGG
AGGTAGGTGA
GGCCGTGGCC
AAACGCTTCC
ACCTACCAGG
CATCCGCCAG
GGCTACGGCC
TGACAGAAAC
AACCAGCGCC
ATTCTGATCA
CCCCCGAAGG
GGACGACAAG
CCTGGCGCAG
TAGGCAAGGT
GGTGCCCTTC
TTCGAGGCTA
AGGTGGTGGA
CTTGGACACC
GGTAAGACAC
TGGGTGTGAA
CCAGCGCGGC
GAGCTGTGCG
TCCGTGGCCC
CATGATCATG
AGCGGCTACG
TTAACAACCC
CGAGGCTACA
AACGCTCTCA
TCGACAAGGA
CGGCTGGCTG
CACAGCGGCG
ACATCGCCTA
CTGGGACGAG
GACGAGCACT
TCTTCATCGT
GGACCGGCTG
AAGAGCCTGA
TCAAATACAA
GGGCTACCAG
GTAGCCCCAG
CCGAACTGGA
GAGCATCCTG
CTGCAACACC
CCAACATCTT
CGACGCCGGG
GTCGCCGGCC
TGCCCGACGA
CGATGCCGGC
GAGCTGCCCG
CCGCAGTCGT
CGTGCTGGAA
CACGGTAAAA
CCATGACCGA
GAAGGAGATC
GTGGACTATG
TGGCCAGCCA
GGTTACAACC
GCCAAGAAGC
TGCGCGGTGG
TGTTGTGTTC
GTGGACGAGG
TGCCTAAAGG
ACTGACCGGC
AAGTTGGACG
CCCGCAAGAT
CCGCGAGATT
CTCATTAAGG
CCAAGAAGGG
CGGCAAGATC
GCCGTGTAAT
TCTAGTTGTT
TAAACGAGCT
CGCTAGCCTC
GAGTCTAGAG
TCGACCTGCA
GG