名称: | pcDNA3.1-WT-VWF |
---|---|
别称: | Plasmid#124794 |
基因长: | 8480bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
反向引物: | BGH:TAGAAGGCACAGTCGAGG |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AATTGCATGA
AGAATCTGCT
TAGGGTTAGG
CGTTTTGCGC
TGCTTCGCGA
TGTACGGGCC
AGATATACGC
GTTGACATTG
ATTATTGACT
AGTTATTAAT
AGTAATCAAT
TACGGGGTCA
TTAGTTCATA
GCCCATATAT
GGAGTTCCGC
GTTACATAAC
TTACGGTAAA
TGGCCCGCCT
GGCTGACCGC
CCAACGACCC
CCGCCCATTG
ACGTCAATAA
TGACGTATGT
TCCCATAGTA
ACGCCAATAG
GGACTTTCCA
TTGACGTCAA
TGGGTGGAGT
ATTTACGGTA
AACTGCCCAC
TTGGCAGTAC
ATCAAGTGTA
TCATATGCCA
AGTACGCCCC
CTATTGACGT
CAATGACGGT
AAATGGCCCG
CCTGGCATTA
TGCCCAGTAC
ATGACCTTAT
GGGACTTTCC
TACTTGGCAG
TACATCTACG
TATTAGTCAT
CGCTATTACC
ATGGTGATGC
GGTTTTGGCA
GTACATCAAT
GGGCGTGGAT
AGCGGTTTGA
CTCACGGGGA
TTTCCAAGTC
TCCACCCCAT
TGACGTCAAT
GGGAGTTTGT
TTTGGCACCA
AAATCAACGG
GACTTTCCAA
AATGTCGTAA
CAACTCCGCC
CCATTGACGC
AAATGGGCGG
TAGGCGTGTA
CGGTGGGAGG
TCTATATAAG
CAGAGCTCTC
TGGCTAACTA
GAGAACCCAC
TGCTTACTGG
CTTATCGAAA
TTAATACGAC
TCACTATAGG
GAGACCCAAG
CTGGCTAGTT
AAGCTTGGTA
CCGAGCTCGG
ATCCACTAGT
CCAGTGTGGT
GGAATTCCCG
CAGCCCTCAT
TTGCAGGGGA
AGATGATTCC
TGCCAGATTT
GCCGGGGTGC
TGCTTGCTCT
GGCCCTCATT
TTGCCAGGGA
CCCTTTGTGC
AGAAGGAACT
CGCGGCAGGT
CATCCACGGC
CCGATGCAGC
CTTTTCGGAA
GTGACTTCGT
CAACACCTTT
GATGGGAGCA
TGTACAGCTT
TGCGGGATAC
TGCAGTTACC
TCCTGGCAGG
GGGCTGCCAG
AAACGCTCCT
TCTCGATTAT
TGGGGACTTC
CAGAATGGCA
AGAGAGTGAG
CCTCTCCGTG
TATCTTGGGG
AATTTTTTGA
CATCCATTTG
TTTGTCAATG
GTACCGTGAC
ACAGGGGGAC
CAAAGAGTCT
CCATGCCCTA
TGCCTCCAAA
GGGCTGTATC
TAGAAACTGA
GGCTGGGTAC
TACAAGCTGT
CCGGTGAGGC
CTATGGCTTT
GTGGCCAGGA
TCGATGGCAG
CGGCAACTTT
CAAGTCCTGC
TGTCAGACAG
ATACTTCAAC
AAGACCTGCG
GGCTGTGTGG
CAACTTTAAC
ATCTTTGCTG
AAGATGACTT
TATGACCCAA
GAAGGGACCT
TGACCTCGGA
CCCTTATGAC
TTTGCCAACT
CATGGGCTCT
GAGCAGTGGA
GAACAGTGGT
GTGAACGGGC
ATCTCCTCCC
AGCAGCTCAT
GCAACATCTC
CTCTGGGGAA
ATGCAGAAGG
GCCTGTGGGA
GCAGTGCCAG
CTTCTGAAGA
GCACCTCGGT
GTTTGCCCGC
TGCCACCCTC
TGGTGGACCC
CGAGCCTTTT
GTGGCCCTGT
GTGAGAAGAC
TTTGTGTGAG
TGTGCTGGGG
GGCTGGAGTG
CGCCTGCCCT
GCCCTCCTGG
AGTACGCCCG
GACCTGTGCC
CAGGAGGGAA
TGGTGCTGTA
CGGCTGGACC
GACCACAGCG
CGTGCAGCCC
AGTGTGCCCT
GCTGGTATGG
AGTATAGGCA
GTGTGTGTCC
CCTTGCGCCA
GGACCTGCCA
GAGCCTGCAC
ATCAATGAAA
TGTGTCAGGA
GCGATGCGTG
GATGGCTGCA
GCTGCCCTGA
GGGACAGCTC
CTGGATGAAG
GCCTCTGCGT
GGAGAGCACC
GAGTGTCCCT
GCGTGCATTC
CGGAAAGCGC
TACCCTCCCG
GCACCTCCCT
CTCTCGAGAC
TGCAACACCT
GCATTTGCCG
AAACAGCCAG
TGGATCTGCA
GCAATGAAGA
ATGTCCAGGG
GAGTGCCTTG
TCACAGGTCA
ATCACACTTC
AAGAGCTTTG
ACAACAGATA
CTTCACCTTC
AGTGGGATCT
GCCAGTACCT
GCTGGCCCGG
GATTGCCAGG
ACCACTCCTT
CTCCATTGTC
ATTGAGACTG
TCCAGTGTGC
TGATGACCGC
GACGCTGTGT
GCACCCGCTC
CGTCACCGTC
CGGCTGCCTG
GCCTGCACAA
CAGCCTTGTG
AAACTGAAGC
ATGGGGCAGG
AGTTGCCATG
GATGGCCAGG
ACGTCCAGCT
CCCCCTCCTG
AAAGGTGACC
TCCGCATCCA
GCATACAGTG
ACGGCCTCCG
TGCGCCTCAG
CTACGGGGAG
GACCTGCAGA
TGGACTGGGA
TGGCCGCGGG
AGGCTGCTGG
TGAAGCTGTC
CCCCGTCTAT
GCCGGGAAGA
CCTGCGGCCT
GTGTGGGAAT
TACAATGGCA
ACCAGGGCGA
CGACTTCCTT
ACCCCCTCTG
GGCTGGCGGA
GCCCCGGGTG
GAGGACTTCG
GGAACGCCTG
GAAGCTGCAC
GGGGACTGCC
AGGACCTGCA
GAAGCAGCAC
AGCGATCCCT
GCGCCCTCAA
CCCGCGCATG
ACCAGGTTCT
CCGAGGAGGC
GTGCGCGGTC
CTGACGTCCC
CCACATTCGA
GGCCTGCCAT
CGTGCCGTCA
GCCCGCTGCC
CTACCTGCGG
AACTGCCGCT
ACGACGTGTG
CTCCTGCTCG
GACGGCCGCG
AGTGCCTGTG
CGGCGCCCTG
GCCAGCTATG
CCGCGGCCTG
CGCGGGGAGA
GGCGTGCGCG
TCGCGTGGCG
CGAGCCAGGC
CGCTGTGAGC
TGAACTGCCC
GAAAGGCCAG
GTGTACCTGC
AGTGCGGGAC
CCCCTGCAAC
CTGACCTGCC
GCTCTCTCTC
TTACCCGGAT
GAGGAATGCA
ATGAGGCCTG
CCTGGAGGGC
TGCTTCTGCC
CCCCAGGGCT
CTACATGGAT
GAGAGGGGGG
ACTGCGTGCC
CAAGGCCCAG
TGCCCCTGTT
ACTATGACGG
TGAGATCTTC
CAGCCAGAAG
ACATCTTCTC
AGACCATCAC
ACCATGTGCT
ACTGTGAGGA
TGGCTTCATG
CACTGTACCA
TGAGTGGAGT
CCCCGGAAGC
TTGCTGCCTG
ACGCTGTCCT
CAGCAGTCCC
CTGTCTCATC
GCAGCAAAAG
GAGCCTATCC
TGTCGGCCCC
CCATGGTCAA
GCTGGTGTGT
CCCGCTGACA
ACCTGCGGGC
TGAAGGGCTC
GAGTGTACCA
AAACGTGCCA
GAACTATGAC
CTGGAGTGCA
TGAGCATGGG
CTGTGTCTCT
GGCTGCCTCT
GCCCCCCGGG
CATGGTCCGG
CATGAGAACA
GATGTGTGGC
CCTGGAAAGG
TGTCCCTGCT
TCCATCAGGG
CAAGGAGTAT
GCCCCTGGAG
AAACAGTGAA
GATTGGCTGC
AACACTTGTG
TCTGTCGGGA
CCGGAAGTGG
AACTGCACAG
ACCATGTGTG
TGATGCCACG
TGCTCCACGA
TCGGCATGGC
CCACTACCTC
ACCTTCGACG
GGCTCAAATA
CCTGTTCCCC
GGGGAGTGCC
AGTACGTTCT
GGTGCAGGAT
TACTGCGGCA
GTAACCCTGG
GACCTTTCGG
ATCCTAGTGG
GGAATAAGGG
ATGCAGCCAC
CCCTCAGTGA
AATGCAAGAA
ACGGGTCACC
ATCCTGGTGG
AGGGAGGAGA
GATTGAGCTG
TTTGACGGGG
AGGTGAATGT
GAAGAGGCCC
ATGAAGGATG
AGACTCACTT
TGAGGTGGTG
GAGTCTGGCC
GGTACATCAT
TCTGCTGCTG
GGCAAAGCCC
TCTCCGTGGT
CTGGGACCGC
CACCTGAGCA
TCTCCGTGGT
CCTGAAGCAG
ACATACCAGG
AGAAAGTGTG
TGGCCTGTGT
GGGAATTTTG
ATGGCATCCA
GAACAATGAC
CTCACCAGCA
GCAACCTCCA
AGTGGAGGAA
GACCCTGTGG
ACTTTGGGAA
CTCCTGGAAA
GTGAGCTCGC
AGTGTGCTGA
CACCAGAAAA
GTGCCTCTGG
ACTCATCCCC
TGCCACCTGC
CATAACAACA
TCATGAAGCA
GACGATGGTG
GATTCCTCCT
GTAGAATCCT
TACCAGTGAC
GTCTTCCAGG
ACTGCAACAA
GCTGGTGGAC
CCCGAGCCAT
ATCTGGATGT
CTGCATTTAC
GACACCTGCT
CCTGTGAGTC
CATTGGGGAC
TGCGCCTGCT
TCTGCGACAC
CATTGCTGCC
TATGCCCACG
TGTGTGCCCA
GCATGGCAAG
GTGGTGACCT
GGAGGACGGC
CACATTGTGC
CCCCAGAGCT
GCGAGGAGAG
GAATCTCCGG
GAGAACGGGT
ATGAGTGTGA
GTGGCGCTAT
AACAGCTGTG
CACCTGCCTG
TCAAGTCACG
TGTCAGCACC
CTGAGCCACT
GGCCTGCCCT
GTGCAGTGTG
TGGAGGGCTG
CCATGCCCAC
TGCCCTCCAG
GGAAAATCCT
GGATGAGCTT
TTGCAGACCT
GCGTTGACCC
TGAAGACTGT
CCAGTGTGTG
AGGTGGCTGG
CCGGCGTTTT
GCCTCAGGAA
AGAAAGTCAC
CTTGAATCCC
AGTGACCCTG
AGCACTGCCA
GATTTGCCAC
TGTGATGTTG
TCAACCTCAC
CTGTGAAGCC
TGCCAGGAGC
CGGGAGGCCT
GGTGGTGCCT
CCCACAGATG
CCCCGGTGAG
CCCCACCACT
CTGTATGTGG
AGGACATCTC
GGAACCGCCG
TTGCACGATT
TCTACTGCAG
CAGGCTACTG
GACCTGGTCT
TCCTGCTGGA
TGGCTCCTCC
AGGCTGTCCG
AGGCTGAGTT
TGAAGTGCTG
AAGGCCTTTG
TGGTGGACAT
GATGGAGCGG
CTGCGCATCT
CCCAGAAGTG
GGTCCGCGTG
GCCGTGGTGG
AGTACCACGA
CGGCTCCCAC
GCCTACATCG
GGCTCAAGGA
CCGGAAGCGA
CCGTCAGAGC
TGCGGCGCAT
TGCCAGCCAG
GTGAAGTATG
CGGGCAGCCA
GGTGGCCTCC
ACCAGCGAGG
TCTTGAAATA
CACACTGTTC
CAAATCTTCA
GCAAGATCGA
CCGCCCTGAA
GCCTCCCGCA
TCGCCCTGCT
CCTGATGGCC
AGCCAGGAGC
CCCAACGGAT
GTCCCGGAAC
TTTGTCCGCT
ACGTCCAGGG
CCTGAAGAAG
AAGAAGGTCA
TTGTGATCCC
GGTGGGCATT
GGGCCCCATG
CCAACCTCAA
GCAGATCCGC
CTCATCGAGA
AGCAGGCCCC
TGAGAACAAG
GCCTTCGTGC
TGAGCAGTGT
GGATGAGCTG
GAGCAGCAAA
GGGACGAGAT
CGTTAGCTAC
CTCTGTGACC
TTGCCCCTGA
AGCCCCTCCT
CCTACTCTGC
CCCCCCACAT
GGCACAAGTC
ACTGTGGGCC
CGGGGCTCTT
GGGGGTTTCG
ACCCTGGGGC
CCAAGAGGAA
CTCCATGGTT
CTGGATGTGG
CGTTCGTCCT
GGAAGGATCG
GACAAAATTG
GTGAAGCCGA
CTTCAACAGG
AGCAAGGAGT
TCATGGAGGA
GGTGATTCAG
CGGATGGATG
TGGGCCAGGA
CAGCATCCAC
GTCACGGTGC
TGCAGTACTC
CTACATGGTG
ACCGTGGAGT
ACCCCTTCAG
CGAGGCACAG
TCCAAAGGGG
ACATCCTGCA
GCGGGTGCGA
GAGATCCGCT
ACCAGGGCGG
CAACAGGACC
AACACTGGGC
TGGCCCTGCG
GTACCTCTCT
GACCACAGCT
TCTTGGTCAG
CCAGGGTGAC
CGGGAGCAGG
CGCCCAACCT
GGTCTACATG
GTCACCGGAA
ATCCTGCCTC
TGATGAGATC
AAGAGGCTGC
CTGGAGACAT
CCAGGTGGTG
CCCATTGGAG
TGGGCCCTAA
TGCCAACGTG
CAGGAGCTGG
AGAGGATTGG
CTGGCCCAAT
GCCCCTATCC
TCATCCAGGA
CTTTGAGACG
CTCCCCCGAG
AGGCTCCTGA
CCTGGTGCTG
CAGAGGTGCT
GCTCCGGAGA
GGGGCTGCAG
ATCCCCACCC
TCTCCCCTGC
ACCTGACTGC
AGCCAGCCCC
TGGACGTGAT
CCTTCTCCTG
GATGGCTCCT
CCAGTTTCCC
AGCTTCTTAT
TTTGATGAAA
TGAAGAGTTT
CGCCAAGGCT
TTCATTTCAA
AAGCCAATAT
AGGGCCTCGT
CTCACTCAGG
TGTCAGTGCT
GCAGTATGGA
AGCATCACCA
CCATTGACGT
GCCATGGAAC
GTGGTCCCGG
AGAAAGCCCA
TTTGCTGAGC
CTTGTGGACG
TCATGCAGCG
GGAGGGAGGC
CCCAGCCAAA
TCGGGGATGC
CTTGGGCTTT
GCTGTGCGAT
ACTTGACTTC
AGAAATGCAT
GGTGCCAGGC
CGGGAGCCTC
AAAGGCGGTG
GTCATCCTGG
TCACGGACGT
CTCTGTGGAT
TCAGTGGATG
CAGCAGCTGA
TGCCGCCAGG
TCCAACAGAG
TGACAGTGTT
CCCTATTGGA
ATTGGAGATC
GCTACGATGC
AGCCCAGCTA
CGGATCTTGG
CAGGCCCAGC
AGGCGACTCC
AACGTGGTGA
AGCTCCAGCG
AATCGAAGAC
CTCCCTACCA
TGGTCACCTT
GGGCAATTCC
TTCCTCCACA
AACTGTGCTC
TGGATTTGTT
AGGATTTGCA
TGGATGAGGA
TGGGAATGAG
AAGAGGCCCG
GGGACGTCTG
GACCTTGCCA
GACCAGTGCC
ACACCGTGAC
TTGCCAGCCA
GATGGCCAGA
CCTTGCTGAA
GAGTCATCGG
GTCAACTGTG
ACCGGGGGCT
GAGGCCTTCG
TGCCCTAACA
GCCAGTCCCC
TGTTAAAGTG
GAAGAGACCT
GTGGCTGCCG
CTGGACCTGC
CCCTGCGTGT
GCACAGGCAG
CTCCACTCGG
CACATCGTGA
CCTTTGATGG
GCAGAATTTC
AAGCTGACTG
GCAGCTGTTC
TTATGTCCTA
TTTCAAAACA
AGGAGCAGGA
CCTGGAGGTG
ATTCTCCATA
ATGGTGCCTG
CAGCCCTGGA
GCAAGGCAGG
GCTGCATGAA
ATCCATCGAG
GTGAAGCACA
GTGCCCTCTC
CGTCGAGCTG
CACAGTGACA
TGGAGGTGAC
GGTGAATGGG
AGACTGGTCT
CTGTTCCTTA
CGTGGGTGGG
AACATGGAAG
TCAACGTTTA
TGGTGCCATC
ATGCATGAGG
TCAGATTCAA
TCACCTTGGT
CACATCTTCA
CATTCACTCC
ACAAAACAAT
GAGTTCCAAC
TGCAGCTCAG
CCCCAAGACT
TTTGCTTCAA
AGACGTATGG
TCTGTGTGGG
ATCTGTGATG
AGAACGGAGC
CAATGACTTC
ATGCTGAGGG
ATGGCACAGT
CACCACAGAC
TGGAAAACAC
TTGTTCAGGA
ATGGACTGTG
CAGCGGCCAG
GGCAGACGTG
CCAGCCCATC
CTGGAGGAGC
AGTGTCTTGT
CCCCGACAGC
TCCCACTGCC
AGGTCCTCCT
CTTACCACTG
TTTGCTGAAT
GCCACAAGGT
CCTGGCTCCA
GCCACATTCT
ATGCCATCTG
CCAGCAGGAC
AGTTGCCACC
AGGAGCAAGT
GTGTGAGGTG
ATCGCCTCTT
ATGCCCACCT
CTGTCGGACC
AACGGGGTCT
GCGTTGACTG
GAGGACACCT
GATTTCTGTG
CTATGTCATG
CCCACCATCT
CTGGTCTACA
ACCACTGTGA
GCATGGCTGT
CCCCGGCACT
GTGATGGCAA
CGTGAGCTCC
TGTGGGGACC
ATCCCTCCGA
AGGCTGTTTC
TGCCCTCCAG
ATAAAGTCAT
GTTGGAAGGC
AGCTGTGTCC
CTGAAGAGGC
CTGCACTCAG
TGCATTGGTG
AGGATGGAGT
CCAGCACCAG
TTCCTGGAAG
CCTGGGTCCC
GGACCACCAG
CCCTGTCAGA
TCTGCACATG
CCTCAGCGGG
CGGAAGGTCA
ACTGCACAAC
GCAGCCCTGC
CCCACGGCCA
AAGCTCCCAC
GTGTGGCCTG
TGTGAAGTAG
CCCGCCTCCG
CCAGAATGCA
GACCAGTGCT
GCCCCGAGTA
TGAGTGTGTG
TGTGACCCAG
TGAGCTGTGA
CCTGCCCCCA
GTGCCTCACT
GTGAACGTGG
CCTCCAGCCC
ACACTGACCA
ACCCTGGCGA
GTGCAGACCC
AACTTCACCT
GCGCCTGCAG
GAAGGAGGAG
TGCAAAAGAG
TGTCCCCACC
CTCCTGCCCC
CCGCACCGTT
TGCCCACCCT
TCGGAAGACC
CAGTGCTGTG
ATGAGTATGA
GTGTGCCTGC
AACTGTGTCA
ACTCCACAGT
GAGCTGTCCC
CTTGGGTACT
TGGCCTCAAC
CGCCACCAAT
GACTGTGGCT
GTACCACAAC
CACCTGCCTT
CCCGACAAGG
TGTGTGTCCA
CCGAAGCACC
ATCTACCCTG
TGGGCCAGTT
CTGGGAGGAG
GGCTGCGATG
TGTGCACCTG
CACCGACATG
GAGGATGCCG
TGATGGGCCT
CCGCGTGGCC
CAGTGCTCCC
AGAAGCCCTG
TGAGGACAGC
TGTCGGTCGG
GCTTCACTTA
CGTTCTGCAT
GAAGGCGAGT
GCTGTGGAAG
GTGCCTGCCA
TCTGCCTGTG
AGGTGGTGAC
TGGCTCACCG
CGGGGGGACT
CCCAGTCTTC
CTGGAAGAGT
GTCGGCTCCC
AGTGGGCCTC
CCCGGAGAAC
CCCTGCCTCA
TCAATGAGTG
TGTCCGAGTG
AAGGAGGAGG
TCTTTATACA
ACAAAGGAAC
GTCTCCTGCC
CCCAGCTGGA
GGTCCCTGTC
TGCCCCTCGG
GCTTTCAGCT
GAGCTGTAAG
ACCTCAGCGT
GCTGCCCAAG
CTGTCGCTGT
GAGCGCATGG
AGGCCTGCAT
GCTCAATGGC
ACTGTCATTG
GGCCCGGGAA
GACTGTGATG
ATCGATGTGT
GCACGACCTG
CCGCTGCATG
GTGCAGGTGG
GGGTCATCTC
TGGATTCAAG
CTGGAGTGCA
GGAAGACCAC
CTGCAACCCC
TGCCCCCTGG
GTTACAAGGA
AGAAAATAAC
ACAGGTGAAT
GTTGTGGGAG
ATGTTTGCCT
ACGGCTTGCA
CCATTCAGCT
AAGAGGAGGA
CAGATCATGA
CACTGAAGCG
TGATGAGACG
CTCCAGGATG
GCTGTGATAC
TCACTTCTGC
AAGGTCAATG
AGAGAGGAGA
GTACTTCTGG
GAGAAGAGGG
TCACAGGCTG
CCCACCCTTT
GATGAACACA
AGTGTCTGGC
TGAGGGAGGT
AAAATTATGA
AAATTCCAGG
CACCTGCTGT
GACACATGTG
AGGAGCCTGA
GTGCAACGAC
ATCACTGCCA
GGCTGCAGTA
TGTCAAGGTG
GGAAGCTGTA
AGTCTGAAGT
AGAGGTGGAT
ATTCACTACT
GCCAGGGCAA
ATGTGCCAGC
AAAGCCATGT
ACTCCATTGA
CATCAACGAT
GTGCAGGACC
AGTGCTCCTG
CTGCTCTCCG
ACACGGACGG
AGCCCATGCA
GGTGGCCCTG
CACTGCACCA
ATGGCTCTGT
TGTGTACCAT
GAGGTTCTCA
ATGCCATGGA
GTGCAAATGC
TCCCCCAGGA
AGTGCAGCAA
GGATATCGCT
AGCGAACAGA
AGCTAATAAG
TGAAGAGGAC
CTAATCTGAG
AATTCAAACC
CGCTGATCAG
CCTCGACTGT
GCCTTCTAGT
TGCCAGCCAT
CTGTTGTTTG
CCCCTCCCCC
GTGCCTTCCT
TGACCCTGGA
AGGTGCCACT
CCCACTGTCC
TTTCCTAATA
AAATGAGGAA
ATTGCATCGC
ATTGTCTGAG
TAGGTGTCAT
TCTATTCTGG
GGGGTGGGGT
GGGGCAGGAC
AGCAAGGGGG
AGGATTGGGA
AGACAATAGC
AGGCATGCTG
GGGATGCGGT
GGGCTCTATG
GCTTCTGAGG
CGGAAAGAAC
CAGCTGGGGC
TCTAGGGGGT
ATCCCCACGC
GCCCTGTAGC
GGCGCATTAA
GCGCGGCGGG
TGTGGTGGTT
ACGCGCAGCG
TGACCGCTAC
ACTTGCCAGC
GCCCTAGCGC
CCGCTCCTTT
CGCTTTCTTC
CCTTCCTTTC
TCGCCACGTT
CGCCGGCTTT
CCCCGTCAAG
CTCTAAATCG
GGGGCTCCCT
TTAGGGTTCC
GATTTAGTGC
TTTACGGCAC
CTCGACCCCA
AAAAACTTGA
TTAGGGTGAT
GGTTCACGTA
GTGGGCCATC
GCCCTGATAG
ACGGTTTTTC
GCCCTTTGAC
GTTGGAGTCC
ACGTTCTTTA
ATAGTGGACT
CTTGTTCCAA
ACTGGAACAA
CACTCAACCC
TATCTCGGTC
TATTCTTTTG
ATTTATAAGG
GATTTTGCCG
ATTTCGGCCT
ATTGGTTAAA
AAATGAGCTG
ATTTAACAAA
AATTTAACGC
GAATTAATTC
TGTGGAATGT
GTGTCAGTTA
GGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCAGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCATAGTC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCTGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGCT
TTTGCAAAAA
GCTCCCGGGA
GCTTGTATAT
CCATTTTCGG
ATCTGATCAA
GAGACAGGAT
GAGGATCGTT
TCGCATGATT
GAACAAGATG
GATTGCACGC
AGGTTCTCCG
GCCGCTTGGG
TGGAGAGGCT
ATTCGGCTAT
GACTGGGCAC
AACAGACAAT
CGGCTGCTCT
GATGCCGCCG
TGTTCCGGCT
GTCAGCGCAG
GGGCGCCCGG
TTCTTTTTGT
CAAGACCGAC
CTGTCCGGTG
CCCTGAATGA
ACTGCAGGAC
GAGGCAGCGC
GGCTATCGTG
GCTGGCCACG
ACGGGCGTTC
CTTGCGCAGC
TGTGCTCGAC
GTTGTCACTG
AAGCGGGAAG
GGACTGGCTG
CTATTGGGCG
AAGTGCCGGG
GCAGGATCTC
CTGTCATCTC
ACCTTGCTCC
TGCCGAGAAA
GTATCCATCA
TGGCTGATGC
AATGCGGCGG
CTGCATACGC
TTGATCCGGC
TACCTGCCCA
TTCGACCACC
AAGCGAAACA
TCGCATCGAG
CGAGCACGTA
CTCGGATGGA
AGCCGGTCTT
GTCGATCAGG
ATGATCTGGA
CGAAGAGCAT
CAGGGGCTCG
CGCCAGCCGA
ACTGTTCGCC
AGGCTCAAGG
CGCGCATGCC
CGACGGCGAG
GATCTCGTCG
TGACCCATGG
CGATGCCTGC
TTGCCGAATA
TCATGGTGGA
AAATGGCCGC
TTTTCTGGAT
TCATCGACTG
TGGCCGGCTG
GGTGTGGCGG
ACCGCTATCA
GGACATAGCG
TTGGCTACCC
GTGATATTGC
TGAAGAGCTT
GGCGGCGAAT
GGGCTGACCG
CTTCCTCGTG
CTTTACGGTA
TCGCCGCTCC
CGATTCGCAG
CGCATCGCCT
TCTATCGCCT
TCTTGACGAG
TTCTTCTGAG
CGGGACTCTG
GGGTTCGCGA
AATGACCGAC
CAAGCGACGC
CCAACCTGCC
ATCACGAGAT
TTCGATTCCA
CCGCCGCCTT
CTATGAAAGG
TTGGGCTTCG
GAATCGTTTT
CCGGGACGCC
GGCTGGATGA
TCCTCCAGCG
CGGGGATCTC
ATGCTGGAGT
TCTTCGCCCA
CCCCAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGTATAC
CGTCGACCTC
TAGCTAGAGC
TTGGCGTAAT
CATGGTCATA
GCTGTTTCCT
GTGTGAAATT
GTTATCCGCT
CACAATTCCA
CACAACATAC
GAGCCGGAAG
CATAAAGTGT
AAAGCCTGGG
GTGCCTAATG
AGTGAGCTAA
CTCACATTAA
TTGCGTTGCG
CTCACTGCCC
GCTTTCCAGT
CGGGAAACCT
GTCGTGCCAG
CTGCATTAAT
GAATCGGCCA
ACGCGCGGGG
AGAGGCGGTT
TGCGTATTGG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGAAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGACG
TCGACGGATC
GGGAGATCTC
CCGATCCCCT
ATGGTGCACT
CTCAGTACAA
TCTGCTCTGA
TGCCGCATAG
TTAAGCCAGT
ATCTGCTCCC
TGCTTGTGTG
TTGGAGGTCG
CTGAGTAGTG
CGCGAGCAAA
ATTTAAGCTA
CAACAAGGCA
AGGCTTGACC
GAC