名称: | p35S-A3A-PBE |
---|---|
别称: | Plasmid#119773 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | 35S |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GAGCTCGGTA
CCCCTACTCC
AAAAATGTCA
AAGATACAGT
CTCAGAAGAC
CAAAGGGCTA
TTGAGACTTT
TCAACAAAGG
GTAATTTCGG
GAAACCTCCT
CGGATTCCAT
TGCCCAGCTA
TCTGTCACTT
CATCGAAAGG
ACAGTAGAAA
AGGAAGGTGG
CTCCTACAAA
TGCCATCATT
GCGATAAAGG
AAAGGCTATC
ATTCAAGATG
CCTCTGCCGA
CAGTGGTCCC
AAAGATGGAC
CCCCACCCAC
GAGGAGCATC
GTGGAAAAAG
AAGACGTTCC
AACCACGTCT
TCAAAGCAAG
TGGATTGATG
TGACATCTCC
ACTGACGTAA
GGGATGACGC
ACAATCCCAC
CCCTACTCCA
AAAATGTCAA
AGATACAGTC
TCAGAAGACC
AAAGGGCTAT
TGAGACTTTT
CAACAAAGGG
TAATTTCGGG
AAACCTCCTC
GGATTCCATT
GCCCAGCTAT
CTGTCACTTC
ATCGAAAGGA
CAGTAGAAAA
GGAAGGTGGC
TCCTACAAAT
GCCATCATTG
CGATAAAGGA
AAGGCTATCA
TTCAAGATGC
CTCTGCCGAC
AGTGGTCCCA
AAGATGGACC
CCCACCCACG
AGGAGCATCG
TGGAAAAAGA
AGACGTTCCA
ACCACGTCTT
CAAAGCAAGT
GGATTGATGT
GACATCTCCA
CTGACGTAAG
GGATGACGCA
CAATCCCACT
ATCCTTCGCA
AGACCCTTCC
TCTATATAAG
GAAGTTCATT
TCATTTGGAG
AGGACAGCCC
AAGCTTGTCG
ACGGATCCAT
GGAGGCCAGC
CCGGCTAGCG
GCCCAAGGCA
TCTCATGGAC
CCGCACATCT
TCACCAGCAA
CTTCAACAAC
GGCATCGGCA
GGCACAAGAC
CTACTTGTGC
TACGAGGTGG
AGAGGCTCGA
CAACGGAACC
TCCGTGAAGA
TGGACCAACA
CAGGGGGTTC
CTCCACAACC
AAGCCAAGAA
CCTCCTCTGC
GGCTTCTACG
GCAGGCACGC
CGAGTTGAGG
TTCCTCGACT
TGGTGCCATC
CCTCCAACTC
GATCCAGCCC
AAATCTACCG
CGTGACCTGG
TTCATCTCCT
GGTCCCCATG
CTTCTCCTGG
GGTTGCGCCG
GCGAGGTTCG
GGCTTTCCTC
CAAGAAAACA
CCCACGTCCG
CCTCCGCATT
TTCGCCGCCA
GGATCTATGA
TTACGACCCT
CTCTACAAGG
AGGCCCTCCA
GATGCTGCGG
GACGCCGGTG
CTCAGGTGAG
TATCATGACC
TACGACGAGT
TCAAGCACTG
CTGGGACACC
TTCGTTGACC
ACCAGGGCTG
CCCATTCCAA
CCATGGGACG
GTCTGGATGA
ACACAGCCAA
GCCTTGTCCG
GCAGGCTCCG
GGCCATCCTC
CAAAACCAGG
GGAACTCCGG
GAGCGAGACG
CCAGGCACCT
CCGAGTCGGC
CACCCCAGAA
TCTCTTAAGG
ACAAGAAGTA
CTCGATCGGC
CTCGCCATCG
GGACGAACTC
AGTTGGCTGG
GCCGTGATCA
CCGACGAGTA
CAAGGTGCCC
TCTAAGAAGT
TCAAGGTCCT
GGGGAACACC
GACCGCCATT
CCATCAAGAA
GAACCTCATC
GGCGCTCTCC
TGTTCGACAG
CGGGGAGACC
GCTGAGGCTA
CGAGGCTCAA
GAGAACCGCT
AGGCGCCGGT
ACACGAGAAG
GAAGAACAGG
ATCTGCTACC
TCCAAGAGAT
TTTCTCCAAC
GAGATGGCCA
AGGTTGACGA
TTCATTCTTC
CACCGCCTGG
AGGAGTCTTT
CCTCGTGGAG
GAGGATAAGA
AGCACGAGCG
GCATCCCATC
TTCGGCAACA
TCGTGGACGA
GGTTGCCTAC
CACGAGAAGT
ACCCTACGAT
CTACCATCTG
CGGAAGAAGC
TCGTGGACTC
CACCGATAAG
GCGGACCTCA
GACTGATCTA
CCTCGCTCTG
GCCCACATGA
TCAAGTTCCG
CGGCCATTTC
CTGATCGAGG
GGGATCTCAA
CCCAGACAAC
AGCGATGTTG
ACAAGCTGTT
CATCCAACTC
GTGCAGACCT
ACAACCAACT
CTTCGAGGAG
AACCCGATCA
ACGCCTCTGG
CGTGGACGCG
AAGGCTATCC
TGTCCGCGAG
GCTCTCGAAG
TCCAGGAGGC
TGGAGAACCT
GATCGCTCAG
CTCCCAGGCG
AGAAGAAGAA
CGGCCTGTTC
GGGAACCTCA
TCGCTCTCAG
CCTGGGGCTC
ACCCCGAACT
TCAAGTCGAA
CTTCGATCTC
GCTGAGGACG
CCAAGCTGCA
ACTCTCCAAG
GACACCTACG
ACGATGACCT
CGATAACCTC
CTGGCCCAGA
TCGGCGATCA
ATACGCGGAC
CTGTTCCTCG
CTGCCAAGAA
CCTGTCGGAC
GCCATCCTCC
TGTCAGATAT
CCTCCGCGTG
AACACCGAGA
TCACGAAGGC
TCCACTCTCT
GCCTCCATGA
TCAAGCGCTA
CGACGAGCAC
CATCAGGATC
TGACCCTCCT
GAAGGCGCTG
GTCCGCCAAC
AGCTCCCGGA
GAAGTACAAG
GAGATTTTCT
TCGATCAGTC
GAAGAACGGC
TACGCTGGGT
ACATCGACGG
CGGGGCCTCA
CAAGAGGAGT
TCTACAAGTT
CATCAAGCCA
ATCCTGGAGA
AGATGGACGG
CACGGAGGAG
CTCCTGGTGA
AGCTCAACAG
GGAGGACCTC
CTGCGGAAGC
AGAGAACCTT
CGATAACGGC
AGCATCCCCC
ACCAAATCCA
TCTCGGGGAG
CTGCACGCCA
TCCTGAGAAG
GCAAGAGGAC
TTCTACCCTT
TCCTCAAGGA
TAACCGGGAG
AAGATCGAGA
AGATCCTGAC
CTTCAGAATC
CCATACTACG
TCGGCCCTCT
CGCGCGGGGG
AACTCAAGAT
TCGCTTGGAT
GACCCGCAAG
TCTGAGGAGA
CCATCACGCC
GTGGAACTTC
GAGGAGGTGG
TGGACAAGGG
CGCTAGCGCT
CAGTCGTTCA
TCGAGAGGAT
GACCAACTTC
GACAAGAACC
TGCCCAACGA
GAAGGTGCTC
CCTAAGCACT
CGCTCCTGTA
CGAGTACTTC
ACCGTCTACA
ACGAGCTCAC
GAAGGTGAAG
TACGTCACCG
AGGGCATGCG
CAAGCCAGCG
TTCCTGTCCG
GGGAGCAGAA
GAAGGCTATC
GTGGACCTCC
TGTTCAAGAC
CAACCGGAAG
GTCACGGTTA
AGCAACTCAA
GGAGGACTAC
TTCAAGAAGA
TCGAGTGCTT
CGATTCGGTC
GAGATCAGCG
GCGTTGAGGA
CCGCTTCAAC
GCCAGCCTCG
GGACCTACCA
CGATCTCCTG
AAGATCATCA
AGGATAAGGA
CTTCCTGGAC
AACGAGGAGA
ACGAGGATAT
CCTGGAGGAC
ATCGTGCTGA
CCCTCACGCT
GTTCGAGGAC
AGGGAGATGA
TCGAGGAGCG
CCTGAAGACG
TACGCCCATC
TCTTCGATGA
CAAGGTCATG
AAGCAACTCA
AGCGCCGGAG
ATACACCGGC
TGGGGGAGGC
TGTCCCGCAA
GCTCATCAAC
GGCATCCGGG
ACAAGCAGTC
CGGGAAGACC
ATCCTCGACT
TCCTCAAGAG
CGATGGCTTC
GCCAACAGGA
ACTTCATGCA
ACTGATCCAC
GATGACAGCC
TCACCTTCAA
GGAGGATATC
CAAAAGGCTC
AAGTGAGCGG
CCAGGGGGAC
TCGCTGCACG
AGCATATCGC
GAACCTCGCT
GGCTCCCCCG
CGATCAAGAA
GGGCATCCTC
CAGACCGTGA
AGGTTGTGGA
CGAGCTCGTG
AAGGTCATGG
GCCGGCACAA
GCCTGAGAAC
ATCGTCATCG
AGATGGCCAG
AGAGAACCAA
ACCACGCAGA
AGGGGCAAAA
GAACTCTAGG
GAGCGCATGA
AGCGCATCGA
GGAGGGCATC
AAGGAGCTGG
GGTCCCAAAT
CCTCAAGGAG
CACCCAGTGG
AGAACACCCA
ACTGCAGAAC
GAGAAGCTCT
ACCTGTACTA
CCTCCAGAAC
GGCAGGGATA
TGTACGTGGA
CCAAGAGCTG
GATATCAACC
GCCTCAGCGA
TTACGACGTC
GATCATATCG
TTCCCCAGTC
TTTCCTGAAG
GATGACTCCA
TCGACAACAA
GGTCCTCACC
AGGTCGGACA
AGAACCGCGG
CAAGTCAGAT
AACGTTCCAT
CTGAGGAGGT
CGTTAAGAAG
ATGAAGAACT
ACTGGAGGCA
GCTCCTGAAC
GCCAAGCTGA
TCACGCAAAG
GAAGTTCGAC
AACCTCACCA
AGGCTGAGAG
AGGCGGGCTC
TCAGAGCTGG
ACAAGGCCGG
CTTCATCAAG
CGGCAGCTGG
TCGAGACCAG
ACAAATCACG
AAGCACGTTG
CGCAAATCCT
CGACTCTCGG
ATGAACACGA
AGTACGATGA
GAACGACAAG
CTGATCAGGG
AGGTTAAGGT
GATCACCCTG
AAGTCTAAGC
TCGTCTCCGA
CTTCAGGAAG
GATTTCCAGT
TCTACAAGGT
TCGCGAGATC
AACAACTACC
ACCATGCCCA
TGACGCTTAC
CTCAACGCTG
TGGTCGGCAC
CGCTCTGATC
AAGAAGTACC
CAAAGCTGGA
GTCCGAGTTC
GTGTACGGGG
ACTACAAGGT
TTACGATGTG
CGCAAGATGA
TCGCCAAGTC
GGAGCAAGAG
ATCGGCAAGG
CTACCGCCAA
GTACTTCTTC
TACTCAAACA
TCATGAACTT
CTTCAAGACC
GAGATCACGC
TGGCCAACGG
CGAGATCCGG
AAGAGACCGC
TCATCGAGAC
CAACGGCGAG
ACGGGGGAGA
TCGTGTGGGA
CAAGGGCAGG
GATTTCGCGA
CCGTCCGCAA
GGTTCTCTCC
ATGCCCCAGG
TGAACATCGT
CAAGAAGACC
GAGGTCCAAA
CGGGCGGGTT
CTCAAAGGAG
TCTATCCTGC
CTAAGCGGAA
CAGCGACAAG
CTCATCGCCA
GAAAGAAGGA
CTGGGACCCA
AAGAAGTACG
GCGGGTTCGA
CAGCCCTACC
GTGGCCTACT
CGGTCCTGGT
TGTGGCGAAG
GTTGAGAAGG
GCAAGTCCAA
GAAGCTCAAG
AGCGTGAAGG
AGCTCCTGGG
GATCACCATC
ATGGAGAGGT
CCAGCTTCGA
GAAGAACCCA
ATCGACTTCC
TGGAGGCCAA
GGGCTACAAG
GAGGTGAAGA
AGGACCTGAT
CATCAAGCTC
CCGAAGTACT
CTCTCTTCGA
GCTGGAGAAC
GGCAGGAAGA
GAATGCTGGC
TTCCGCTGGC
GAGCTCCAGA
AGGGGAACGA
GCTCGCGCTG
CCAAGCAAGT
ACGTGAACTT
CCTCTACCTG
GCTTCCCACT
ACGAGAAGCT
CAAGGGCAGC
CCGGAGGACA
ACGAGCAAAA
GCAGCTGTTC
GTCGAGCAGC
ACAAGCATTA
CCTCGACGAG
ATCATCGAGC
AAATCTCCGA
GTTCAGCAAG
CGCGTGATCC
TCGCCGACGC
GAACCTGGAT
AAGGTCCTCT
CCGCCTACAA
CAAGCACCGG
GACAAGCCCA
TCAGAGAGCA
AGCGGAGAAC
ATCATCCATC
TCTTCACCCT
GACGAACCTC
GGCGCTCCTG
CTGCTTTCAA
GTACTTCGAC
ACCACGATCG
ATCGGAAGAG
ATACACCTCC
ACGAAGAAGG
TCCTGGACGC
GACCCTCATC
CACCAGTCGA
TCACCGGCCT
GTACGAGACG
AGGATCGACC
TCTCACAACT
CGGCGGGGAT
AAGAGACCCG
CAGCAACCAA
GAAGGCAGGG
CAAGCAAAGA
AGAAGAAGAC
GCGTGACTCC
GGCGGCAGCA
CCAACCTGTC
CGACATCATC
GAGAAGGAGA
CGGGCAAGCA
ACTCGTGATC
CAGGAGAGCA
TCCTCATGCT
GCCAGAGGAG
GTGGAGGAGG
TCATCGGCAA
CAAGCCAGAG
TCCGACATCC
TGGTGCACAC
CGCCTACGAC
GAGTCCACCG
ACGAGAACGT
CATGCTCCTG
ACCAGCGACG
CCCCAGAGTA
CAAGCCATGG
GCCCTCGTCA
TCCAGGACAG
CAACGGGGAG
AACAAGATCA
AGATGCTGTC
GGGGGGGAGC
CCAAAGAAGA
AGCGGAAGGT
GTAGTGTACA
AGTAACCCGG
GAATTCGGTA
CGCTGAAATC
ACCAGTCTCT
CTCTACAAAT
CTATCTCTCT
CTATTTTCTC
CATAAATAAT
GTGTGAGTAG
TTTCCCGATA
AGGGAAATTA
GGGTTCTTAT
AGGGTTTCGC
TCATGTGTTG
AGCATATAAG
AAACCCTTAG
TATGTATTTG
TATTTGTAAA
ATACTTCTAT
CAATAAAATT
TCTAATTCCT
AAAACCAAAA
TCCAGTACTA
AAATCCAGAT
CTCCTAAAGT
CCCTATAGAT
CTTTGTCGTG
AATATAAACC
AGACACGAGA
CGACTAAACC
TGGAGCCCAG
ACGCCGTTCG
AAGCTAGAAG
TACCGCTTAG
GCAGGAGGCC
GTTAGGGAAA
AGATGCTAAG
GCAGGGTTGG
TTACGTTGAC
TCCCCCGTAG
GTTTGGTTTA
AATATGATGA
AGTGGACGGA
AGGAAGGAGG
AAGACAAGGA
AGGATAAGGT
TGCAGGCCCT
GTGCAAGGTA
AGAAGATGGA
AATTTGATAG
AGGTACGCTA
CTATACTTAT
ACTATACGCT
AAGGGAATGC
TTGTATTTAT
ACCCTATACC
CCCTAATAAC
CCCTTATCAA
TTTAAGAAAT
AATCCGCATA
AGCCCCCGCT
TAAAAATTGG
TATCAGAGCC
ATGAATAGGT
CTATGACCAA
AACTCAAGAG
GATAAAACCT
CACCAAAATA
CGAAAGAGTT
CTTAACTCTA
AAGATAAAAG
ATCTTTCAAG
ATCAAAACTA
GTTCCCTCAC
ACCGGTGACG
GGGATCGCAT
GCGATATCTC
GAGATCTAGC
TTGGCGTAAT
CATGGTCATA
GCTGTTTCCT
GTGTGAAATT
GTTATCCGCT
CACAATTCCA
CACAACATAC
GAGCCGGAAG
CATAAAGTGT
AAAGCCTGGG
GTGCCTAATG
AGTGAGCTAA
CTCACATTAA
TTGCGTTGCG
CTCACTGCCC
GCTTTCCAGT
CGGGAAACCT
GTCGTGCCAG
CTGCATTAAT
GAATCGGCCA
ACGCGCGGGG
AGAGGCGGTT
TGCGTATTGG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGAAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTG