名称: | pGL3-mArg1 promoter/enhancer -31/-3810 |
---|---|
别称: | Plasmid#34571 |
基因长: | 3779bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 启动子检测 |
片段类型: | Promoter |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | Fluc |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 30度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTGACGCGCC
CTGTAGCGGC
GCATTAAGCG
CGGCGGGTGT
GGTGGTTACG
CGCAGCGTGA
CCGCTACACT
TGCCAGCGCC
CTAGCGCCCG
CTCCTTTCGC
TTTCTTCCCT
TCCTTTCTCG
CCACGTTCGC
CGGCTTTCCC
CGTCAAGCTC
TAAATCGGGG
GCTCCCTTTA
GGGTTCCGAT
TTAGTGCTTT
ACGGCACCTC
GACCCCAAAA
AACTTGATTA
GGGTGATGGT
TCACGTAGTG
GGCCATCGCC
CTGATAGACG
GTTTTTCGCC
CTTTGACGTT
GGAGTCCACG
TTCTTTAATA
GTGGACTCTT
GTTCCAAACT
GGAACAACAC
TCAACCCTAT
CTCGGTCTAT
TCTTTTGATT
TATAAGGGAT
TTTGCCGATT
TCGGCCTATT
GGTTAAAAAA
TGAGCTGATT
TAACAAAAAT
TTAACGCGAA
TTTTAACAAA
ATATTAACGC
TTACAATTTG
CCATTCGCCA
TTCAGGCTGC
GCAACTGTTG
GGAAGGGCGA
TCGGTGCGGG
CCTCTTCGCT
ATTACGCCAG
CCCAAGCTAC
CATGATAAGT
AAGTAATATT
AAGGTACGGG
AGGTACTTGG
AGCGGCCGCA
ATAAAATATC
TTTATTTTCA
TTACATCTGT
GTGTTGGTTT
TTTGTGTGAA
TCGATAGTAC
TAACATACGC
TCTCCATCAA
AACAAAACGA
AACAAAACAA
ACTAGCAAAA
TAGGCTGTCC
CCAGTGCAAG
TGCAGGTGCC
AGAACATTTC
TCTATCGATA
GGTACCGAGC
TCTTACGCGT
AGATTGCCAG
GAATATACCA
GACACTGGAG
CTAGGTAAAG
GCCTCCTGTA
CACCCATACA
AGGAGCGCTT
CTCTGCCTTC
AAATATCAAC
AAGTTTTAAA
TCCCCAGTGT
GTAGTACAGA
GCAGGCTGGG
TGGATTTGAG
GTCTGTGTTC
TTAGGAATTG
AACCTTTAAA
GCGCAGTATA
CATTCTCTAC
GCTAAGGAAA
GACTTGGGCT
GGGAAAAGTG
GGCTCTGCCA
AGACTCAGGC
CGATGACCTC
GTCCCCTCCA
GAGCAGTGGG
TCTCAAGCTT
CCTAACTCTC
CAACCTTTTA
ATACAGTTCT
TATGTTGCAG
TGGCCCCAAT
CATCAAATTG
TTTTGTTGTT
ACAGCATAAC
TATAATTTTC
CTAGTTATAA
AGCATACTGT
AAACAACTGT
GTTTTCTGAT
GGTTTTAGGG
TAACCGCTGT
GAAAGGATCT
ATCAGCCCCA
AAAGGAATTG
GGATCCACAG
GTTAAGAACC
AGTGCCTTAG
ATGTTCTGAG
GCCACAGCTT
TGGAGATTGA
AAAGACAGTC
CTTTGTGAAG
ACTAGAATCT
TAGGCCAAGG
TGCTTGCTGC
CAAGCCTGAC
ACCCCAGTCT
GACTCTCAGA
ACCCATACAG
CAAGGAGAAC
CTCCTCAGGG
CCATGGTATG
TGTTTCCCAA
CACGGACACA
CACACAATCA
CACAGAGTAA
AGGGTTTTTT
AATTAAGCAA
GAAATACAGT
TTACACATAT
GTCTGCATAA
GGTCACGGAG
GGTGGTAGCC
GACGAGAGAC
CAGCTCATCT
TCAATAAGGA
AGTCAGAGAG
CAGAAGGCTT
TGTCAGCAGG
GCAAGACTAT
ACTTTGTTAG
GAAGTGAGGC
ATTGTTCAGA
CTTCCTTATG
CTTTCTTATG
AACAGGCTGT
ATTAGCCAAC
AGTCCTGTCT
GTACGTGAGT
TGTGCCACTT
TGGGTTGAGG
GTGCTGGGCT
AACACAGATC
TTAGTTAAGC
ATTTCCTTGT
CCTTTGATTC
CTCAATGACC
CAGAACAGGC
AAACAATACG
ATACTTATGT
TTGATGAATT
AGATGACTGC
ATCTACAACT
GAACTTGTGC
ACTGAGTTCT
GGATGGTCGC
CTGAAGGAGA
ACGCAGGGAG
GCAGGCGATA
CTTTAATAGG
ACTGACTGTG
AGGGTAAATC
GGTGTCATAC
ATGGAACCGT
CGTGTGTATG
GAGATACCCA
GAACAATAAA
GACCCAAGAA
AAACAGATAG
TCAAGAAATC
ATGAGTTTTA
CTTAATCAGG
GCGTTTTGAT
CAGTCAGTCC
AATGATGCCA
ATGATGAAAA
GTCACCCTCA
AACTGCCCGA
GGGAAGACTT
TCAGGAAAAG
CCCTGGTGAT
AAAGTTTAAC
AGCAAATACC
TATGAATGAA
TGTATAAAGC
TAGTAACGGC
CGTAGCACTG
TTTTCAAAAC
GTAGTCACCG
GCTCACTCTA
CATTTGCGAG
ACCCCGACAG
AAGTAAATGT
AAGGTCAAGC
GATTTTGAAA
AAGAGGAGCA
CCAGTTCCTT
TCCTGCAGCT
CTAACGTCCT
ACCAATGGGG
CTGCATGGAC
TGGTGCAGCC
ACAGGTGATG
AGCCCAGAGC
ATAGAGGAAA
CTGGAAGATG
GAGCAGCGTA
TCGTTTATAA
CAGCAACTTG
GCATCTGATG
GAGATAGCTC
AGTGGTAACG
TGGGGAGTCT
GTCCATTTGG
TGCTGGTGGG
GAAACTGGGA
GAGACAAAGG
GGAAATGGAG
GATTTAAACA
AAATACAAAA
AAAAAAAATG
GACGCAACAG
TCAGCAATCA
CTGTGAATAG
CAAATTCTTC
ATGCATCCAA
GACTTAAGCC
CAGCATCTTT
CAGGCAGGAA
AACAATCTAC
CCCTGAATGG
CATTTTTCTT
CTTTTCTTTT
TACCTTTGGG
GCAGGTTCTG
TGTTGACCTT
TGAACTAACC
CTATAACCCC
AGCAGGTGTT
GAACCTATAA
TCCCCCTGCC
TCATCCTCTC
AAATAGCTGG
AATTTGTGGG
CCAGCACCAA
GAGGCCCAAA
GAAAGAGAAA
GTTTAAATTA
AATAGAAGAA
AATGTAGAGG
TATTGATAGG
TGGTGGTATC
ACACACCTTT
AATCCCAGCA
CTCAGGAGGC
AGTGACAGAT
TGCTATCTGT
GAGTTCAAGG
TTAGGCTGGT
CTATAGGGCT
AGTTCTAGGG
TAGCCAGAGC
CACACAAAGA
AAACATGTCT
CCAAAAAGCA
AACAAAAATG
TAGAGGTGCA
CATTTGGGAT
CTGGGAGCAG
GTAAAACTTA
AATTCCTAGC
TAGCTTCAAA
TATATAGGAA
AATTCTATCT
TACATAAATC
AATTTTTTAA
AAAAATCTAT
AACCGTATAT
CAGAAAAACA
TAGTAAGATG
AAAAATATAT
TTGAAAAGGA
GGTACCAAAA
TGGTCCTGAG
GGACTTTGGC
TTCTTGATTT
ATTCATTCAA
TAAATGCAAT
ACATTTATTC
AGTCAATAAG
TCAATGTTTA
TTTTGTATTG
AACGATTCTA
TGTACTGATG
GTAGAGGGAA
CAGAATCTTT
CTTGAGGTAT
TAAAATTCTG
GGTGCTTTTT
GTCCTCACAA
GGTTAAAGAA
ATTTACTTCT
GACATGTGGG
TTTTCTTTTT
AAAATTATAA
TCATGTAACT
TTGTAACTGG
GAGAATACTT
GGTTGTGTTT
CTTCTAAATT
AATACTTTTA
AAAATATTTC
TTTAGTGTTG
GGGGTGGCTT
TACACAGGGA
CCGGACCACA
TCACCCACGT
GGAGGTCAGA
GATCAACTTG
GAGGAGTTGA
CTCTTTCCTT
GCACCAAATG
GGTTCTGCGG
GTCAAACTCG
GGTCATCGGG
CTTGGTGTTA
CCGGCTGGCC
TCTCTCATCT
GCCCTAGATC
TCAGGGGGCC
CTGCTGACAC
TCTGCTGGTG
TGTAGATTAG
AATCGCGGCA
GCTCGGCGGG
TGCAGAATGC
CCATTGCTCC
GTTTTGATTC
TTCTGCAACC
TGTATGTGAC
TAGCAACCTC
ACCAAGCTGC
AGTCTCTTCC
TCGGCAAAAC
AGCATAAGAA
TGATTCTCAG
TCTGAAGTTA
GTTTAAAGAG
GAAACAGGAA
ACGAAAGAAC
ATTAACTAGA
ATAGCACTTG
GCACACGACA
GACAACTCAT
ATTAGCTAGT
TTGCTTTCCC
TATCTTGGGA
TATCATGATT
CCAAAAATGA
GATTTTCCCG
GAGACTGTAT
TTCAAAAATA
GAACTGCTTT
GGGTTGTCAG
GGAAATAAAT
GATGCCTTCC
TGAAATAATA
ATAATAATAA
CAACAACAAC
AACAACAACA
ATATAAAACA
AAAACCAGCC
CCATGCTTTC
CTAGACAGTG
TAACCTGGTG
ACACATGAAA
TGTGTCTCAC
TTTCCCCAGA
ACTTGAAGCC
TTGACTCAGG
ATGCTCAACA
GGAGGGAAGT
AAGAGACACC
ACCCCCCACC
CCCGCTTGCT
GTTTTAGCCT
CACCTTGCAA
GTCCCCAGTG
GACTTAAATC
TTGGAAAAGG
TGAGCACCCT
GCCCTGAGGT
GCCCAGGCCG
GAAGCCTAGC
ACTTCACATG
AGGTTATGAA
ATCACACATA
ATTGTCAATT
GTCTGAGGAG
AGATTAATGT
CATCCAGCTG
GCTTTTTCAA
AAGGGTGTGA
ACTGGACGGA
TGAATAATGC
TCAGAGGGAA
GAATGGTAGT
TCCTCTGATG
GGGAGGTTCT
GTTGACTCTG
TCATTCTTCC
ATTCGGTGGG
CGGAGCCAGT
TGTTGGATAA
ACAGATCCAA
CCTGATTATA
AGGGGGGAAA
AAGATGTGCC
CTCTGTCTTT
TAGGGTTACG
GCCGGTGGAG
AGGAGCTGGA
CAGCCCGAGC
ACATGCAGCC
TCGAGATCTG
CGATCTAAGT
AAGCTTGGCA
TTCCGGTACT
GTTGGTAAAG
CCACCATGGA
AGACGCCAAA
AACATAAAGA
AAGGCCCGGC
GCCATTCTAT
CCGCTGGAAG
ATGGAACCGC
TGGAGAGCAA
CTGCATAAGG
CTATGAAGAG
ATACGCCCTG
GTTCCTGGAA
CAATTGCTTT
TACAGATGCA
CATATCGAGG
TGGACATCAC
TTACGCTGAG
TACTTCGAAA
TGTCCGTTCG
GTTGGCAGAA
GCTATGAAAC
GATATGGGCT
GAATACAAAT
CACAGAATCG
TCGTATGCAG
TGAAAACTCT
CTTCAATTCT
TTATGCCGGT
GTTGGGCGCG
TTATTTATCG
GAGTTGCAGT
TGCGCCCGCG
AACGACATTT
ATAATGAACG
TGAATTGCTC
AACAGTATGG
GCATTTCGCA
GCCTACCGTG
GTGTTCGTTT
CCAAAAAGGG
GTTGCAAAAA
ATTTTGAACG
TGCAAAAAAA
GCTCCCAATC
ATCCAAAAAA
TTATTATCAT
GGATTCTAAA
ACGGATTACC
AGGGATTTCA
GTCGATGTAC
ACGTTCGTCA
CATCTCATCT
ACCTCCCGGT
TTTAATGAAT
ACGATTTTGT
GCCAGAGTCC
TTCGATAGGG
ACAAGACAAT
TGCACTGATC
ATGAACTCCT
CTGGATCTAC
TGGTCTGCCT
AAAGGTGTCG
CTCTGCCTCA
TAGAACTGCC
TGCGTGAGAT
TCTCGCATGC
CAGAGATCCT
ATTTTTGGCA
ATCAAATCAT
TCCGGATACT
GCGATTTTAA
GTGTTGTTCC
ATTCCATCAC
GGTTTTGGAA
TGTTTACTAC
ACTCGGATAT
TTGATATGTG
GATTTCGAGT
CGTCTTAATG
TATAGATTTG
AAGAAGAGCT
GTTTCTGAGG
AGCCTTCAGG
ATTACAAGAT
TCAAAGTGCG
CTGCTGGTGC
CAACCCTATT
CTCCTTCTTC
GCCAAAAGCA
CTCTGATTGA
CAAATACGAT
TTATCTAATT
TACACGAAAT
TGCTTCTGGT
GGCGCTCCCC
TCTCTAAGGA
AGTCGGGGAA
GCGGTTGCCA
AGAGGTTCCA
TCTGCCAGGT
ATCAGGCAAG
GATATGGGCT
CACTGAGACT
ACATCAGCTA
TTCTGATTAC
ACCCGAGGGG
GATGATAAAC
CGGGCGCGGT
CGGTAAAGTT
GTTCCATTTT
TTGAAGCGAA
GGTTGTGGAT
CTGGATACCG
GGAAAACGCT
GGGCGTTAAT
CAAAGAGGCG
AACTGTGTGT
GAGAGGTCCT
ATGATTATGT
CCGGTTATGT
AAACAATCCG
GAAGCGACCA
ACGCCTTGAT
TGACAAGGAT
GGATGGCTAC
ATTCTGGAGA
CATAGCTTAC
TGGGACGAAG
ACGAACACTT
CTTCATCGTT
GACCGCCTGA
AGTCTCTGAT
TAAGTACAAA
GGCTATCAGG
TGGCTCCCGC
TGAATTGGAA
TCCATCTTGC
TCCAACACCC
CAACATCTTC
GACGCAGGTG
TCGCAGGTCT
TCCCGACGAT
GACGCCGGTG
AACTTCCCGC
CGCCGTTGTT
GTTTTGGAGC
ACGGAAAGAC
GATGACGGAA
AAAGAGATCG
TGGATTACGT
CGCCAGTCAA
GTAACAACCG
CGAAAAAGTT
GCGCGGAGGA
GTTGTGTTTG
TGGACGAAGT
ACCGAAAGGT
CTTACCGGAA
AACTCGACGC
AAGAAAAATC
AGAGAGATCC
TCATAAAGGC
CAAGAAGGGC
GGAAAGATCG
CCGTGTAATT
CTAGAGTCGG
GGCGGCCGGC
CGCTTCGAGC
AGACATGATA
AGATACATTG
ATGAGTTTGG
ACAAACCACA
ACTAGAATGC
AGTGAAAAAA
ATGCTTTATT
TGTGAAATTT
GTGATGCTAT
TGCTTTATTT
GTAACCATTA
TAAGCTGCAA
TAAACAAGTT
AACAACAACA
ATTGCATTCA
TTTTATGTTT
CAGGTTCAGG
GGGAGGTGTG
GGAGGTTTTT
TAAAGCAAGT
AAAACCTCTA
CAAATGTGGT
AAAATCGATA
AGGATCCGTC
GACCGATGCC
CTTGAGAGCC
TTCAACCCAG
TCAGCTCCTT
CCGGTGGGCG
CGGGGCATGA
CTATCGTCGC
CGCACTTATG
ACTGTCTTCT
TTATCATGCA
ACTCGTAGGA
CAGGTGCCGG
CAGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCA