产品货号：P45762
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pU6-CGAS(human)-sgRNA1-sgRNA2-Cas9-EGFP-Puro |
|---|---|
| 别称: | |
| 基因长: | |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | 绿色 |
| 启动子: | U6 |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
GAGGGCCTAT
TTCCCATGAT
TCCTTCATAT
TTGCATATAC
GATACAAGGC
TGTTAGAGAG
ATAATTGGAA
TTAATTTGAC
TGTAAACACA
AAGATATTAG
TACAAAATAC
GTGACGTAGA
AAGTAATAAT
TTCTTGGGTA
GTTTGCAGTT
TTAAAATTAT
GTTTTAAAAT
GGACTATCAT
ATGCTTACCG
TAACTTGAAA
GTATTTCGAT
TTCTTGGCTT
TATATATCTT
GTGGAAAGGA
CGAAACACCG
CCGCGATGAT
ATCTCCACGG
GTTTTAGAGC
TAGAAATAGC
AAGTTAAAAT
AAGGCTAGTC
CGTTATCAAC
TTGAAAAAGT
GGCACCGAGT
CGGTGCTTTT
TTGTTTTAGA
GCTAGAAATA
GCAAGTTAAA
ATAAGGCTAG
TCCGTTTTTA
GCGCGTGCGC
CAATTCTGCA
GACAAATGGC
TCTAGAATCA
CTAGGGGTTC
CTGCGGCCGC
ACGCGTGAGG
GCCTATTTCC
CATGATTCCT
TCATATTTGC
ATATACGATA
CAAGGCTGTT
AGAGAGATAA
TTGGAATTAA
TTTGACTGTA
AACACAAAGA
TATTAGTACA
AAATACGTGA
CGTAGAAAGT
AATAATTTCT
TGGGTAGTTT
GCAGTTTTAA
AATTATGTTT
TAAAATGGAC
TATCATATGC
TTACCGTAAC
TTGAAAGTAT
TTCGATTTCT
TGGCTTTATA
TATCTTGTGG
AAAGGACGAA
ACACCGGCTT
CCGCACGGAA
TGCCAGGTTT
TAGAGCTAGA
AATAGCAAGT
TAAAATAAGG
CTAGTCCGTT
ATCAACTTGA
AAAAGTGGCA
CCGAGTCGGT
GCTTTTTTTG
GTACCCGTTA
CATAACTTAC
GGTAAATGGC
CCGCCTGGCT
GACCGCCCAA
CGACCCCCGC
CCATTGACGT
CAATAGTAAC
GCCAATAGGG
ACTTTCCATT
GACGTCAATG
GGTGGAGTAT
TTACGGTAAA
CTGCCCACTT
GGCAGTACAT
CAAGTGTATC
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTGTG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTCGAGGTG
AGCCCCACGT
TCTGCTTCAC
TCTCCCCATC
TCCCCCCCCT
CCCCACCCCC
AATTTTGTAT
TTATTTATTT
TTTAATTATT
TTGTGCAGCG
ATGGGGGCGG
GGGGGGGGGG
GGGGCGCGCG
CCAGGCGGGG
CGGGGCGGGG
CGAGGGGCGG
GGCGGGGCGA
GGCGGAGAGG
TGCGGCGGCA
GCCAATCAGA
GCGGCGCGCT
CCGAAAGTTT
CCTTTTATGG
CGAGGCGGCG
GCGGCGGCGG
CCCTATAAAA
AGCGAAGCGC
GCGGCGGGCG
GGAGTCGCTG
CGACGCTGCC
TTCGCCCCGT
GCCCCGCTCC
GCCGCCGCCT
CGCGCCGCCC
GCCCCGGCTC
TGACTGACCG
CGTTACTCCC
ACAGGTGAGC
GGGCGGGACG
GCCCTTCTCC
TCCGGGCTGT
AATTAGCTGA
GCAAGAGGTA
AGGGTTTAAG
GGATGGTTGG
TTGGTGGGGT
ATTAATGTTT
AATTACCTGG
AGCACCTGCC
TGAAATCACT
TTTTTTCAGG
TTGGACCGGT
GCCACCATGG
ACTATAAGGA
CCACGACGGA
GACTACAAGG
ATCATGATAT
TGATTACAAA
GACGATGACG
ATAAGATGGC
CCCAAAGAAG
AAGCGGAAGG
TCGGTATCCA
CGGAGTCCCA
GCAGCCGACA
AGAAGTACAG
CATCGGCCTG
GACATCGGCA
CCAACTCTGT
GGGCTGGGCC
GTGATCACCG
ACGAGTACAA
GGTGCCCAGC
AAGAAATTCA
AGGTGCTGGG
CAACACCGAC
CGGCACAGCA
TCAAGAAGAA
CCTGATCGGA
GCCCTGCTGT
TCGACAGCGG
CGAAACAGCC
GAGGCCACCC
GGCTGAAGAG
AACCGCCAGA
AGAAGATACA
CCAGACGGAA
GAACCGGATC
TGCTATCTGC
AAGAGATCTT
CAGCAACGAG
ATGGCCAAGG
TGGACGACAG
CTTCTTCCAC
AGACTGGAAG
AGTCCTTCCT
GGTGGAAGAG
GATAAGAAGC
ACGAGCGGCA
CCCCATCTTC
GGCAACATCG
TGGACGAGGT
GGCCTACCAC
GAGAAGTACC
CCACCATCTA
CCACCTGAGA
AAGAAACTGG
TGGACAGCAC
CGACAAGGCC
GACCTGCGGC
TGATCTATCT
GGCCCTGGCC
CACATGATCA
AGTTCCGGGG
CCACTTCCTG
ATCGAGGGCG
ACCTGAACCC
CGACAACAGC
GACGTGGACA
AGCTGTTCAT
CCAGCTGGTG
CAGACCTACA
ACCAGCTGTT
CGAGGAAAAC
CCCATCAACG
CCAGCGGCGT
GGACGCCAAG
GCCATCCTGT
CTGCCAGACT
GAGCAAGAGC
AGACGGCTGG
AAAATCTGAT
CGCCCAGCTG
CCCGGCGAGA
AGAAGAATGG
CCTGTTCGGA
AACCTGATTG
CCCTGAGCCT
GGGCCTGACC
CCCAACTTCA
AGAGCAACTT
CGACCTGGCC
GAGGATGCCA
AACTGCAGCT
GAGCAAGGAC
ACCTACGACG
ACGACCTGGA
CAACCTGCTG
GCCCAGATCG
GCGACCAGTA
CGCCGACCTG
TTTCTGGCCG
CCAAGAACCT
GTCCGACGCC
ATCCTGCTGA
GCGACATCCT
GAGAGTGAAC
ACCGAGATCA
CCAAGGCCCC
CCTGAGCGCC
TCTATGATCA
AGAGATACGA
CGAGCACCAC
CAGGACCTGA
CCCTGCTGAA
AGCTCTCGTG
CGGCAGCAGC
TGCCTGAGAA
GTACAAAGAG
ATTTTCTTCG
ACCAGAGCAA
GAACGGCTAC
GCCGGCTACA
TTGACGGCGG
AGCCAGCCAG
GAAGAGTTCT
ACAAGTTCAT
CAAGCCCATC
CTGGAAAAGA
TGGACGGCAC
CGAGGAACTG
CTCGTGAAGC
TGAACAGAGA
GGACCTGCTG
CGGAAGCAGC
GGACCTTCGA
CAACGGCAGC
ATCCCCCACC
AGATCCACCT
GGGAGAGCTG
CACGCCATTC
TGCGGCGGCA
GGAAGATTTT
TACCCATTCC
TGAAGGACAA
CCGGGAAAAG
ATCGAGAAGA
TCCTGACCTT
CCGCATCCCC
TACTACGTGG
GCCCTCTGGC
CAGGGGAAAC
AGCAGATTCG
CCTGGATGAC
CAGAAAGAGC
GAGGAAACCA
TCACCCCCTG
GAACTTCGAG
GAAGTGGTGG
ACAAGGGCGC
TTCCGCCCAG
AGCTTCATCG
AGCGGATGAC
CAACTTCGAT
AAGAACCTGC
CCAACGAGAA
GGTGCTGCCC
AAGCACAGCC
TGCTGTACGA
GTACTTCACC
GTGTATAACG
AGCTGACCAA
AGTGAAATAC
GTGACCGAGG
GAATGAGAAA
GCCCGCCTTC
CTGAGCGGCG
AGCAGAAAAA
GGCCATCGTG
GACCTGCTGT
TCAAGACCAA
CCGGAAAGTG
ACCGTGAAGC
AGCTGAAAGA
GGACTACTTC
AAGAAAATCG
AGTGCTTCGA
CTCCGTGGAA
ATCTCCGGCG
TGGAAGATCG
GTTCAACGCC
TCCCTGGGCA
CATACCACGA
TCTGCTGAAA
ATTATCAAGG
ACAAGGACTT
CCTGGACAAT
GAGGAAAACG
AGGACATTCT
GGAAGATATC
GTGCTGACCC
TGACACTGTT
TGAGGACAGA
GAGATGATCG
AGGAACGGCT
GAAAACCTAT
GCCCACCTGT
TCGACGACAA
AGTGATGAAG
CAGCTGAAGC
GGCGGAGATA
CACCGGCTGG
GGCAGGCTGA
GCCGGAAGCT
GATCAACGGC
ATCCGGGACA
AGCAGTCCGG
CAAGACAATC
CTGGATTTCC
TGAAGTCCGA
CGGCTTCGCC
AACAGAAACT
TCATGCAGCT
GATCCACGAC
GACAGCCTGA
CCTTTAAAGA
GGACATCCAG
AAAGCCCAGG
TGTCCGGCCA
GGGCGATAGC
CTGCACGAGC
ACATTGCCAA
TCTGGCCGGC
AGCCCCGCCA
TTAAGAAGGG
CATCCTGCAG
ACAGTGAAGG
TGGTGGACGA
GCTCGTGAAA
GTGATGGGCC
GGCACAAGCC
CGAGAACATC
GTGATCGAAA
TGGCCAGAGA
GAACCAGACC
ACCCAGAAGG
GACAGAAGAA
CAGCCGCGAG
AGAATGAAGC
GGATCGAAGA
GGGCATCAAA
GAGCTGGGCA
GCCAGATCCT
GAAAGAACAC
CCCGTGGAAA
ACACCCAGCT
GCAGAACGAG
AAGCTGTACC
TGTACTACCT
GCAGAATGGG
CGGGATATGT
ACGTGGACCA
GGAACTGGAC
ATCAACCGGC
TGTCCGACTA
CGATGTGGAC
CATATCGTGC
CTCAGAGCTT
TCTGAAGGAC
GACTCCATCG
ACAACAAGGT
GCTGACCAGA
AGCGACAAGA
ACCGGGGCAA
GAGCGACAAC
GTGCCCTCCG
AAGAGGTCGT
GAAGAAGATG
AAGAACTACT
GGCGGCAGCT
GCTGAACGCC
AAGCTGATTA
CCCAGAGAAA
GTTCGACAAT
CTGACCAAGG
CCGAGAGAGG
CGGCCTGAGC
GAACTGGATA
AGGCCGGCTT
CATCAAGAGA
CAGCTGGTGG
AAACCCGGCA
GATCACAAAG
CACGTGGCAC
AGATCCTGGA
CTCCCGGATG
AACACTAAGT
ACGACGAGAA
TGACAAGCTG
ATCCGGGAAG
TGAAAGTGAT
CACCCTGAAG
TCCAAGCTGG
TGTCCGATTT
CCGGAAGGAT
TTCCAGTTTT
ACAAAGTGCG
CGAGATCAAC
AACTACCACC
ACGCCCACGA
CGCCTACCTG
AACGCCGTCG
TGGGAACCGC
CCTGATCAAA
AAGTACCCTA
AGCTGGAAAG
CGAGTTCGTG
TACGGCGACT
ACAAGGTGTA
CGACGTGCGG
AAGATGATCG
CCAAGAGCGA
GCAGGAAATC
GGCAAGGCTA
CCGCCAAGTA
CTTCTTCTAC
AGCAACATCA
TGAACTTTTT
CAAGACCGAG
ATTACCCTGG
CCAACGGCGA
GATCCGGAAG
CGGCCTCTGA
TCGAGACAAA
CGGCGAAACC
GGGGAGATCG
TGTGGGATAA
GGGCCGGGAT
TTTGCCACCG
TGCGGAAAGT
GCTGAGCATG
CCCCAAGTGA
ATATCGTGAA
AAAGACCGAG
GTGCAGACAG
GCGGCTTCAG
CAAAGAGTCT
ATCCTGCCCA
AGAGGAACAG
CGATAAGCTG
ATCGCCAGAA
AGAAGGACTG
GGACCCTAAG
AAGTACGGCG
GCTTCGACAG
CCCCACCGTG
GCCTATTCTG
TGCTGGTGGT
GGCCAAAGTG
GAAAAGGGCA
AGTCCAAGAA
ACTGAAGAGT
GTGAAAGAGC
TGCTGGGGAT
CACCATCATG
GAAAGAAGCA
GCTTCGAGAA
GAATCCCATC
GACTTTCTGG
AAGCCAAGGG
CTACAAAGAA
GTGAAAAAGG
ACCTGATCAT
CAAGCTGCCT
AAGTACTCCC
TGTTCGAGCT
GGAAAACGGC
CGGAAGAGAA
TGCTGGCCTC
TGCCGGCGAA
CTGCAGAAGG
GAAACGAACT
GGCCCTGCCC
TCCAAATATG
TGAACTTCCT
GTACCTGGCC
AGCCACTATG
AGAAGCTGAA
GGGCTCCCCC
GAGGATAATG
AGCAGAAACA
GCTGTTTGTG
GAACAGCACA
AGCACTACCT
GGACGAGATC
ATCGAGCAGA
TCAGCGAGTT
CTCCAAGAGA
GTGATCCTGG
CCGACGCTAA
TCTGGACAAA
GTGCTGTCCG
CCTACAACAA
GCACCGGGAT
AAGCCCATCA
GAGAGCAGGC
CGAGAATATC
ATCCACCTGT
TTACCCTGAC
CAATCTGGGA
GCCCCTGCCG
CCTTCAAGTA
CTTTGACACC
ACCATCGACC
GGAAGAGGTA
CACCAGCACC
AAAGAGGTGC
TGGACGCCAC
CCTGATCCAC
CAGAGCATCA
CCGGCCTGTA
CGAGACACGG
ATCGACCTGT
CTCAGCTGGG
AGGCGACAAA
AGGCCGGCGG
CCACGAAAAA
GGCCGGCCAG
GCAAAAAAGA
AAAAGGAATT
CGGCAGTGGA
GAGGGCAGAG
GAAGTCTGCT
AACATGCGGT
GACGTCGAGG
AGAATCCTGG
CCCAGTGAGC
AAGGGCGAGG
AGCTGTTCAC
CGGGGTGGTG
CCCATCCTGG
TCGAGCTGGA
CGGCGACGTA
AACGGCCACA
AGTTCAGCGT
GTCCGGCGAG
GGCGAGGGCG
ATGCCACCTA
CGGCAAGCTG
ACCCTGAAGT
TCATCTGCAC
CACCGGCAAG
CTGCCCGTGC
CCTGGCCCAC
CCTCGTGACC
ACCCTGACCT
ACGGCGTGCA
GTGCTTCAGC
CGCTACCCCG
ACCACATGAA
GCAGCACGAC
TTCTTCAAGT
CCGCCATGCC
CGAAGGCTAC
GTCCAGGAGC
GCACCATCTT
CTTCAAGGAC
GACGGCAACT
ACAAGACCCG
CGCCGAGGTG
AAGTTCGAGG
GCGACACCCT
GGTGAACCGC
ATCGAGCTGA
AGGGCATCGA
CTTCAAGGAG
GACGGCAACA
TCCTGGGGCA
CAAGCTGGAG
TACAACTACA
ACAGCCACAA
CGTCTATATC
ATGGCCGACA
AGCAGAAGAA
CGGCATCAAG
GTGAACTTCA
AGATCCGCCA
CAACATCGAG
GACGGCAGCG
TGCAGCTCGC
CGACCACTAC
CAGCAGAACA
CCCCCATCGG
CGACGGCCCC
GTGCTGCTGC
CCGACAACCA
CTACCTGAGC
ACCCAGTCCG
CCCTGAGCAA
AGACCCCAAC
GAGAAGCGCG
ATCACATGGT
CCTGCTGGAG
TTCGTGACCG
CCGCCGGGAT
CACTCTCGGC
ATGGACGAGC
TGTACAAGGA
ATTCTAACTA
GAGCTCGCTG
ATCAGCCTCG
ACTGTGCCTT
CTAGTTGCCA
GCCATCTGTT
GTTTGCCCCT
CCCCCGTGCC
TTCCTTGACC
CTGGAAGGTG
CCACTCCCAC
TGTCCTTTCC
TAATAAAATG
AGGAAATTGC
ATCGCATTGT
CTGAGTAGGT
GTCATTCTAT
TCTGGGGGGT
GGGGTGGGGC
AGGACAGCAA
GGGGGAGGAT
TGGGAAGAGA
ATAGCAGGCA
TGCTGGGGAG
CGGCCGCAGG
AACCCCTAGT
GATGGAGTTG
GCCACTCCCT
CTCTGCGCGC
TCGCTCGCTC
ACTGAGGCCG
GGCGACCAAA
GGTCGCCCGA
CGCCCGGGCT
TTGCCCGGGC
GGCCTCAGTG
AGCGAGCGAG
CGCGCAGCTG
CCTGCAGGGG
CGCCTGATGC
GGTATTTTCT
CCTTACGCAT
CTGTGCGGTA
TTTCACACCG
CATACGTCAA
AGCAACCATA
GTACGCGCCC
TGTAGCGGCG
CATTAAGCGC
GGCGGGTGTG
GTGGTTACGC
GCAGCGTGAC
CGCTACACTT
GCCAGCGCCC
TAGCGCCCGC
TCCTTTCGCT
TTCTTCCCTT
CCTTTCTCGC
CACGTTCGCC
GGCTTTCCCC
GTCAAGCTCT
AAATCGGGGG
CTCCCTTTAG
GGTTCCGATT
TAGTGCTTTA
CGGCACCTCG
ACCCCAAAAA
ACTTGATTTG
GGTGATGGTT
CACGTAGTGG
GCCATCGCCC
TGATAGACGG
TTTTTCGCCC
TTTGACGTTG
GAGTCCACGT
TCTTTAATAG
TGGACTCTTG
TTCCAAACTG
GAACAACACT
CAACCCTATC
TCGGGCTATT
CTTTTGATTT
ATAAGGGATT
TTGCCGATTT
CGGCCTATTG
GTTAAAAAAT
GAGCTGATTT
AACAAAAATT
TAACGCGAAT
TTTAACAAAA
TATTAACGTT
TACAATTTTA
TGGTGCACTC
TCAGTACAAT
CTGCTCTGAT
GCCGCATAGT
TAAGCCAGCC
CCGACACCCG
CCAACACCCG
CTGACGCGCC
CTGACGGGCT
TGTCTGCTCC
CGGCATCCGC
TTACAGACAA
GCTGTGACCG
TCTCCGGGAG
GTGTGTCAGT
TAGGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCAGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCATAG
TCCCGCCCCT
AACTCCGCCC
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCT
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGG
CTTTTGCAAA
AAGCTCCCGG
GATGACCGAG
TACAAGCCCA
CGGTGCGCCT
CGCCACCCGC
GACGACGTCC
CCAGGGCCGT
ACGCACCCTC
GCCGCCGCGT
TCGCCGACTA
CCCCGCCACG
CGCCACACCG
TCGATCCGGA
CCGCCACATC
GAGCGGGTCA
CCGAGCTGCA
AGAACTCTTC
CTCACGCGCG
TCGGGCTCGA
CATCGGCAAG
GTGTGGGTCG
CGGACGACGG
CGCCGCGGTG
GCGGTCTGGA
CCACGCCGGA
GAGCGTCGAA
GCGGGGGCGG
TGTTCGCCGA
GATCGGCCCG
CGCATGGCCG
AGTTGAGCGG
TTCCCGGCTG
GCCGCGCAGC
AACAGATGGA
AGGCCTCCTG
GCGCCGCACC
GGCCCAAGGA
GCCCGCGTGG
TTCCTGGCCA
CCGTCGGAGT
CTCGCCCGAC
CACCAGGGCA
AGGGTCTGGG
CAGCGCCGTC
GTGCTCCCCG
GAGTGGAGGC
GGCCGAGCGC
GCCGGGGTGC
CCGCCTTCCT
GGAGACCTCC
GCGCCCCGCA
ACCTCCCCTT
CTACGAGCGG
CTCGGCTTCA
CCGTCACCGC
CGACGTCGAG
GTGCCCGAAG
GACCGCGCAC
CTGGTGCATG
ACCCGCAAGC
CCGGTGCCTG
ACACGTGCTA
CGAGATTTCG
ATTCCACCGC
CGCCTTCTAT
GAAAGGTTGG
GCTTCGGAAT
CGTTTTCCGG
GACGCCGGCT
GGATGATCCT
CCAGCGCGGG
GATCTCATGC
TGGAGTTCTT
CGCCCACCCC
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TAACGCGCGA
GACGAAAGGG
CCTCGTGATA
CGCCTATTTT
TATAGGTTAA
TGTCATGATA
ATAATGGTTT
CTTAGACGTC
AGGTGGCACT
TTTCGGGGAA
ATGTGCGCGG
AACCCCTATT
TGTTTATTTT
TCTAAATACA
TTCAAATATG
TATCCGCTCA
TGAGACAATA
ACCCTGATAA
ATGCTTCAAT
AATATTGAAA
AAGGAAGAGT
ATGAGTATTC
AACATTTCCG
TGTCGCCCTT
ATTCCCTTTT
TTGCGGCATT
TTGCCTTCCT
GTTTTTGCTC
ACCCAGAAAC
GCTGGTGAAA
GTAAAAGATG
CTGAAGATCA
GTTGGGTGCA
CGAGTGGGTT
ACATCGAACT
GGATCTCAAC
AGCGGTAAGA
TCCTTGAGAG
TTTTCGCCCC
GAAGAACGTT
TTCCAATGAT
GAGCACTTTT
AAAGTTCTGC
TATGTGGCGC
GGTATTATCC
CGTATTGACG
CCGGGCAAGA
GCAACTCGGT
CGCCGCATAC
ACTATTCTCA
GAATGACTTG
GTTGAGTACT
CACCAGTCAC
AGAAAAGCAT
CTTACGGATG
GCATGACAGT
AAGAGAATTA
TGCAGTGCTG
CCATAACCAT
GAGTGATAAC
ACTGCGGCCA
ACTTACTTCT
GACAACGATC
GGAGGACCGA
AGGAGCTAAC
CGCTTTTTTG
CACAACATGG
GGGATCATGT
AACTCGCCTT
GATCGTTGGG
AACCGGAGCT
GAATGAAGCC
ATACCAAACG
ACGAGCGTGA
CACCACGATG
CCTGTAGCAA
TGGCAACAAC
GTTGCGCAAA
CTATTAACTG
GCGAACTACT
TACTCTAGCT
TCCCGGCAAC
AATTAATAGA
CTGGATGGAG
GCGGATAAAG
TTGCAGGACC
ACTTCTGCGC
TCGGCCCTTC
CGGCTGGCTG
GTTTATTGCT
GATAAATCTG
GAGCCGGTGA
GCGTGGAAGC
CGCGGTATCA
TTGCAGCACT
GGGGCCAGAT
GGTAAGCCCT
CCCGTATCGT
AGTTATCTAC
ACGACGGGGA
GTCAGGCAAC
TATGGATGAA
CGAAATAGAC
AGATCGCTGA
GATAGGTGCC
TCACTGATTA
AGCATTGGTA
ACTGTCAGAC
CAAGTTTACT
CATATATACT
TTAGATTGAT
TTAAAACTTC
ATTTTTAATT
TAAAAGGATC
TAGGTGAAGA
TCCTTTTTGA
TAATCTCATG
ACCAAAATCC
CTTAACGTGA
GTTTTCGTTC
CACTGAGCGT
CAGACCCCGT
AGAAAAGATC
AAAGGATCTT
CTTGAGATCC
TTTTTTTCTG
CGCGTAATCT
GCTGCTTGCA
AACAAAAAAA
CCACCGCTAC
CAGCGGTGGT
TTGTTTGCCG
GATCAAGAGC
TACCAACTCT
TTTTCCGAAG
GTAACTGGCT
TCAGCAGAGC
GCAGATACCA
AATACTGTCC
TTCTAGTGTA
GCCGTAGTTA
GGCCACCACT
TCAAGAACTC
TGTAGCACCG
CCTACATACC
TCGCTCTGCT
AATCCTGTTA
CCAGTGGCTG
CTGCCAGTGG
CGATAAGTCG
TGTCTTACCG
GGTTGGACTC
AAGACGATAG
TTACCGGATA
AGGCGCAGCG
GTCGGGCTGA
ACGGGGGGTT
CGTGCACACA
GCCCAGCTTG
GAGCGAACGA
CCTACACCGA
ACTGAGATAC
CTACAGCGTG
AGCTATGAGA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
T
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