名称: | pLV3-CMV-CRYBG2(human)-3×FLAG-Fluc-CopGFP-Puro |
---|---|
别称: | NM_001039775.4;AIM1L |
基因长: | 4986bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色,Fluc |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTTAACTTT
TAAAAGAAAA
GGGGGGATTG
GGGGGTACAG
TGCAGGGGAA
AGAATAGTAG
ACATAATAGC
AACAGACATA
CAAACTAAAG
AATTACAAAA
ACAAATTACA
AAAATTCAAA
ATTTTATCGA
TACTAGTATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCG
TTTAGTGAAC
CGTCAGATCG
CCTGGAGACG
CCATCCACGC
TGTTTTGACC
TCCATAGAAG
ATTCTAGAGC
CACCATGGAG
GAGGCAGGTG
GGCCCATGGC
CCGGGCCAAG
GCCCGAGTGG
TAAGTGCCAC
ATTGACATGG
CGGCAGCGGC
CCCCCACCCA
GGAAGAGATC
AAACATGGTT
TTCACAAGGT
GTCCCTGGTG
TCAGGGGCCC
AGATGGAAGC
CCCGCAGAAG
GAGATGTTTG
AGTTCAGCCG
TCGAGAGGAA
GTGGAAGTCA
ATGGCTTTGC
AACACAGGAA
GAAGAGACTG
TGAATTGCCA
GGGCCCTCGG
GATACAGCTG
GCTCCAAGAA
CTTCCAGAGC
CATGGACCCA
TCTTTTCCAA
GAAGTACATA
CCACCTCCCA
AGGAGAAAAG
GCCTGAGGGG
AGGCTGAAGG
AGGCTGTGGA
CCAGAGTGAT
GGCAGCCGCC
AAGCTCCCAG
GACTGAGCCC
CCATGTGTGG
GAGCTATGGC
CAGGACTGAG
CTTTTGGTTC
CCCTGCCTGG
GCCCCGAGAG
CCAAGTCCCC
ACCCAGGTGT
AGGTCTCACC
AGTGGTAGCT
CCCGGAGCCT
CGAGGAATAC
CGAGTGACAC
GCACTGTGCG
GACCACCACA
GTGGTGGGAG
GTCATGTGGA
CCGGCGGATG
AGCAGCTCTG
TGACTGTGAG
GCCAGTGTCC
TCAGGCGAGG
CCCTGCCACG
GGGCCGTCAG
GTCTCCCGCA
TGGTGCCGCC
AGTGGTGGTG
GGCTCCCCAC
CAGGCTCGCC
CAGCCGCAGC
CAGGCCGTGA
AAGTGCTAAG
TAACCTCGTG
CCTGCTGGGC
ACAGCCCCCC
TGCCAGTCAC
CTGCCCAGGC
CCACGGCTGG
CGGGCCAAGG
AGCACAGGTC
TGGGCAGCAC
AGTGGGGGCA
GCCTTGAGGC
AGCTGCCTGA
GACCGGGACA
GCAGAGCTTA
AAGACAGCTC
TGCCCTGGCC
TCTACAGGCA
TCCCAGCCAG
TGCTCACCTG
CCTAAGAATC
AGGATGCCCC
TGCAGCCTGT
CCGGACAGAG
ACCAGGGCAG
AGCCCCGGAT
GCCAGGGCCT
GTGAGCTCTG
GCAGGTGCTG
GGAGCCCCCA
GTTCCACTGA
GCTCCCTCTC
CAGACTTCTC
AGGGTCAAGC
ATCAGTCCCC
TCCTCTCCCA
GACTCGAGAC
CCATGTCCCC
TCTCCTGGCC
TAACTCACCC
TGCAAAGCAG
CCTGTGGTGC
CCACTCACCC
CGGGGCCCGG
CTCACTCCCC
TTGTTCTGCC
CCCTAAAAAA
AAGGACGGGC
CCGTGGACCC
CCCTGCTGCC
ACCGTCCTGC
CCATGGTGAG
GAGCGAGCAT
GTGACAGTCC
CTGGACAACC
TCCTGCTCCA
TCCACTACAA
GAAGGAAGGA
TGTCCCCAGC
CCAGGAGGTC
TTTCTGCTCC
ATCGTCCCCA
AGGAATAAGT
TTGTTCAGAA
CTCTGAAAAT
GTCCCTGTCC
TCCCCTTTAC
CCAGAGGGAG
GTCGTGAAGG
GCCCCGGTGC
TCCTGCTGCC
TCATCTCCCA
CCCGGAAGGA
GGTGGTGCAG
GGGTCCAGTG
CTTCTGCTGC
CTCGTCCCCC
ACCTGGAAAG
AGGTCGTGAA
GGGCCCTGGT
GCTCCTGCTG
CCTCATCTCC
CACCCAGAAG
GAGGTGGTGC
AGGGGTCCAG
TGCTCCTGCT
GCCTTGTTCC
CCACCTGGAA
AGAGGTCGTG
AAGGGCCCCG
GTGCTCCTGA
TGCCTCATTT
CCCACCTGGA
AAGAGGTTGT
GAAGGGCCCT
GGTGCTCCTG
CTGCCTCATC
CCCCACCCAG
AAGGAGGTGG
TGCAGGGGTC
TGGTGCTCCT
GCTGCCTTGT
CCACCACCCC
AAAAGAGGTT
GTGAAGGGCC
CTGGTGCTCC
TGCTGCCTCA
TCGCCCACCC
AGAAAGAGGT
TGTGAAGGGC
CCCTGTGCTC
CTGCTGCCTC
ATCTCCCACC
CAGAAGGAGG
TGGTGCAGGG
GTCTGGTGCT
CCTGCTGCCT
TGTCCCCCAA
GTCAACTGAG
GTTGTCCAGG
GCCCAAAAGG
CAGCAGTAGC
ATCCAAAAAG
AGGCTGTCCA
GGGTATTGCA
GGCAGCCTTG
CCCCGCCCCT
CACCAAGGAA
GAGACTGTTC
AAGGCCCAAT
TGCTCCTGCC
ACCTCACTCC
CCAAACAGGA
TAAGGGGGTC
CAGGACTCTG
AAGGGAGCCC
CATCTCATCT
CTCACCCAGA
AAGAGGTTGT
GCAGGACCCT
GATGCTCTCC
CTGCCCCATC
TTCCTCAGTG
GACAGGGTGT
CCCCCAGCCC
AGGAGGCACC
CCTGCCCCAG
TGCCAACAGG
AGCAGAGGCC
AGCACGGAGT
CCCAGCTTGT
CTCTGATCCC
ACCGAGGGCA
AGACGTGCAC
AGAGACATCA
AGGGAGGAGG
ATGAGGTGGC
CCTGGCCGCT
GACCTGGAGA
TATTCCTGGA
TACGCTGCGG
AGCATGGAGC
CCCCTGAGAT
CCTCCGCACT
CACCGGCTGC
CACGAGCCCC
TCGCTCCTCC
TACCTGTCCA
TGTACGCCAC
GCTGCCTGCC
ATTGAGGAGG
ACCAGCTGGG
GCCATGGGTG
CTGGGCCCCG
GACCCCAGGA
GGTGCCTTCA
CTGGAAGAGA
AAGAGGAGGA
GGAGGAGGAG
GAACCAGAGA
ACCCCTATCT
GAGTGACGAT
GAGAAGCTCC
AGCGCAGGCA
GGAGAAAGCT
GGGCCCAGCC
CCTCCAGGGA
CCTCCACCCT
GCCAGACCCA
CCCAGGTCTC
CTGCTCTCCT
CTGGAGATGA
TGAAGAAGCA
TGTAGCAGGA
ACCAAGGGCC
CCCACTCAGA
GCTGGGATTG
GAACTGCAGG
GAGGCAGCAG
GCCCACTTCC
CGTCTTGGAG
GCAGCCTTCT
GTTCGGCAGT
CTGGTGCCTA
CCGCCAAGGA
GGCCTCCACC
CCGGAACCGC
TGGGCACAAA
ACTATCTGCT
CTGCTGCCCC
ATGGGGCACC
GGGGCTCAGG
AAGGTGCCAG
GACAGTTGCC
CCTGCTTTGC
AGTGAAAGAT
CATCCCCAAC
GGAGAAGCTT
GCCTGTTCCC
TGCCCCTGGA
GGGGTGGAGC
CCAGCCTTAA
AGACCCAGGG
CAAGCTGAAC
ACCAGGCCTG
GAAAGGTGAT
CTTCTTCTCA
GAGTCTGGCT
GCCAAGGCAG
TGGCAGGGAG
GTCTGGGGAG
ACATCGTTGA
TGCCTCAGGC
TGGGCCCCCG
TAGCCTCCAT
AAGGGTAGTT
CGAGGCTGCT
GGGTGCTGTA
CGAAGAGCCA
GAGTTCCGGG
GTCAGAAGCT
GGTCCTGCCT
GAAGGAGACA
TGGAACTCAG
AACCCCAGGG
ACAAAGTGGA
GTCCCCAAGG
CATCGGCTCC
CTAAGGAGGG
TTGTCTGGGA
CTACAGCACC
CCGGAAATCA
GCCTCTTCTC
TGAGGAGGGC
CTCAAGGGGG
AGCAAGTGAA
GCTAACAGAG
GCCTTGAAGA
ATTCCCAGGG
TCTGGAGAAG
CCCCTGCAGG
TGGCATCTGC
CACCGTCTCT
GCAGGACTGT
GGCTACTGTA
CCCCAAACCA
TTATTCGAAG
ACACTCCCTA
CATCCTGGAA
CCTGGAGAGT
ACCCCACCTC
AGAGGCCTGG
GGCACATCGG
ACCCCAGCGT
GGGCTCCCTG
AAGCCCATGA
GATTGGGCTG
CCCAAGTGTG
GAGAAGCCAG
GGGAGCCCAG
GGCTGTGGTG
TATGAGGCCC
CAGGCTTTCA
GGGCCGCAGC
TGGGAAGTGA
GCCGAGACAT
CTACAACCTT
CAGCAGCCAG
AGGACAGCCA
GAGCCCCCAC
CTGGCCTCTG
TGGGGTCCCT
GAGAGTTCTC
GGAGGCTGCT
GGGTGGGCTA
CGAGAAGGAG
GGCTTCCGGG
GCCACCAGTA
TCTGCTGGAG
GAGGGGGAAT
ACCCAGACTG
GTCACACTGG
GGAGGCTATG
ACGAGTTGCT
GACCTCCCTC
CGGGTCATCC
GGACGGACTT
CGGGGACCCG
GCCGTCGTGC
TATTTGAGGC
CATGGACTTC
GAGGGGCACG
GCGTGGAGGT
GAGCAAGGCA
TTGCCGGATG
TGGAGCTGGT
GCAACACGGC
CCCAGCACAC
AGGCCATCCA
CGTGCTCAGC
GGCGTGTGGG
TGGCCTACCA
GGAGGTGGGC
TTCTCCGGGG
AACAGTACGT
GCTGGAGAAG
GGCGTGTATC
GTAACTGCGA
GGACTGGGGC
GCTGGCAACA
GCACCCTCGC
CTCGCTGCAG
CCGGTCCTAC
AGGTCGGGGA
GCACGATCTG
CACTTCGTCT
CAAAGATTCA
GCTTTTCTCC
CGCCCCGACT
TTCTGGGCGA
CCACTTCTCT
TTCGAAGATG
ACCAGGCCGC
TCTGCCCGCC
TCCTTCCGAC
CTCAGTCCTG
CCGGGTCCAC
GGCGGCAGCT
GGATCCTGTT
TGATGAGACG
AACTTCGAGG
GTGACCAGCA
CATTCTCTCT
GAGGGCGAGT
TCCCCACTCT
CACGGCCATG
GGCTGCCTCG
CCTCCACAGT
CCTGGGCTCT
CTCCAGAAGG
TATCCCTGCA
CTTTTCAGAG
CCTTCCATTT
TCCTGTATGG
ACTCGAGTGC
TTCGAGGGGA
AGGAGATCGA
GCTCAGCAGG
GAGGTGCGGA
GCCTGCAAGC
CGAGGGCTTC
AACAACCATG
TGCTGTCTGT
GCGGATCAAG
GGGGGCATTT
GGGTGCTATG
TGAACACAGT
GACTTCCGGG
GCCGCCAGTG
GCTGGTGGGA
AGCTGCGAGA
TCACCAACTG
GCTGACCTAC
AGCGGCACCC
AGAGGGTGGG
CTCCCTCTAC
CCCATCAAGC
AGCGCCGGGT
TTATTTCCGC
CTCTGGAATG
CAGCACTGGG
GGGATTCCTG
GCAGTGCCGG
ACCATGTGGA
GGACATGAAA
GCAGGCCGTG
TGGTGGTCGC
CGACCCCCAA
GCTGGAGGTA
GCTGCATCTG
GTACTACGAG
GATGGGCTGC
TGAAGAACCA
GATGGCCCCC
ACCATGAGCC
TACAGGTGAT
TGGACCCCCT
AGCCCAGGCT
CCAAGGTGGT
GCTGTGGGCC
GAGAGCCGCC
TGCCGCGCCA
GACGTGGAGC
ATCAGTGAAT
CGGGCCACAT
CTGCAGCCAG
ATGTTCGAAG
GCCAGATCCT
GGACGTGAAG
GGAGGCCGGG
GCTACGACCG
GGACCACGTG
GTGCTATGGG
AGCCGGATGA
GGACAGGGCA
TCCCAGATCT
GGACTATCCA
CGTGCTTGAA
TTCGACTACA
AAGACCATGA
CGGTGATTAT
AAAGATCATG
ACATCGACTA
CAAGGATGAC
GATGACAAGG
CCACCAACTT
CAGCCTGCTG
AAGCAGGCCG
GCGACGTGGA
GGAGAACCCC
GGCCCCGCCA
CCATGGAAGA
CGCCAAAAAC
ATAAAGAAAG
GCCCGGCGCC
ATTCTATCCG
CTAGAGGATG
GAACCGCTGG
AGAGCAACTG
CATAAGGCTA
TGAAGAGATA
CGCCCTGGTT
CCTGGAACAA
TTGCTTTTAC
AGATGCACAT
ATCGAGGTGA
ACATCACGTA
CGCGGAATAC
TTCGAAATGT
CCGTTCGGTT
GGCAGAAGCT
ATGAAACGAT
ATGGGCTGAA
TACAAATCAC
AGAATCGTCG
TATGCAGTGA
AAACTCTCTT
CAATTCTTTA
TGCCGGTGTT
GGGCGCGTTA
TTTATCGGAG
TTGCAGTTGC
GCCCGCGAAC
GACATTTATA
ATGAACGTGA
ATTGCTCAAC
AGTATGAACA
TTTCGCAGCC
TACCGTAGTG
TTTGTTTCCA
AAAAGGGGTT
GCAAAAAATT
TTGAACGTGC
AAAAAAAATT
ACCAATAATC
CAGAAAATTA
TTATCATGGA
TTCTAAAACG
GATTACCAGG
GATTTCAGTC
GATGTACACG
TTCGTCACAT
CTCATCTACC
TCCCGGTTTT
AATGAATACG
ATTTTGTACC
AGAGTCCTTT
GATCGTGACA
AAACAATTGC
ACTGATAATG
AACTCCTCTG
GATCTACTGG
GTTACCTAAG
GGTGTGGCCC
TTCCGCATAG
AACTGCCTGC
GTCAGATTCT
CGCATGCCAG
AGATCCTATT
TTTGGCAATC
AAATCATTCC
GGATACTGCG
ATTTTAAGTG
TTGTTCCATT
CCATCACGGT
TTTGGAATGT
TTACTACACT
CGGATATTTG
ATATGTGGAT
TTCGAGTCGT
CTTAATGTAT
AGATTTGAAG
AAGAGCTGTT
TTTACGATCC
CTTCAGGATT
ACAAAATTCA
AAGTGCGTTG
CTAGTACCAA
CCCTATTTTC
ATTCTTCGCC
AAAAGCACTC
TGATTGACAA
ATACGATTTA
TCTAATTTAC
ACGAAATTGC
TTCTGGGGGC
GCACCTCTTT
CGAAAGAAGT
CGGGGAAGCG
GTTGCAAAAC
GCTTCCATCT
TCCAGGGATA
CGACAAGGAT
ATGGGCTCAC
TGAGACTACA
TCAGCTATTC
TGATTACACC
CGAGGGGGAT
GATAAACCGG
GCGCGGTCGG
TAAAGTTGTT
CCATTTTTTG
AAGCGAAGGT
TGTGGATCTG
GATACCGGGA
AAACGCTGGG
CGTTAATCAG
AGAGGCGAAT
TATGTGTCAG
AGGACCTATG
ATTATGTCCG
GTTATGTAAA
CAATCCGGAA
GCGACCAACG
CCTTGATTGA
CAAGGATGGA
TGGCTACATT
CTGGAGACAT
AGCTTACTGG
GACGAAGACG
AACACTTCTT
CATAGTTGAC
CGCTTGAAGT
CTTTAATTAA
ATACAAAGGA
TACCAGGTGG
CCCCCGCTGA
ATTGGAGTCG
ATATTGTTAC
AACACCCCAA
CATCTTCGAC
GCGGGCGTGG
CAGGTCTTCC
CGACGATGAC
GCCGGTGAAC
TTCCCGCCGC
CGTTGTTGTT
TTGGAGCACG
GAAAGACGAT
GACGGAAAAA
GAGATCGTGG
ATTACGTCGC
CAGTCAAGTA
ACAACCGCGA
AAAAGTTGCG
CGGAGGAGTT
GTGTTTGTGG
ACGAAGTACC
GAAAGGTCTT
ACCGGAAAAC
TCGACGCAAG
AAAAATCAGA
GAGATCCTCA
TAAAGGCCAA
GAAGGGCGGA
AAGTCCAAAT
TGTGAGGATC
CGCGGCCGCA
AGGATCTGCG
ATCGCTCCGG
TGCCCGTCAG
TGGGCAGAGC
GCACATCGCC
CACAGTCCCC
GAGAAGTTGG
GGGGAGGGGT
CGGCAATTGA
ACGGGTGCCT
AGAGAAGGTG
GCGCGGGGTA
AACTGGGAAA
GTGATGTCGT
GTACTGGCTC
CGCCTTTTTC
CCGAGGGTGG
GGGAGAACCG
TATATAAGTG
CAGTAGTCGC
CGTGAACGTT
CTTTTTCGCA
ACGGGTTTGC
CGCCAGAACA
CAGCTGAAGC
TTCGAGGGGC
TCGCATCTCT
CCTTCACGCG
CCCGCCGCCC
TACCTGAGGC
CGCCATCCAC
GCCGGTTGAG
TCGCGTTCTG
CCGCCTCCCG
CCTGTGGTGC
CTCCTGAACT
GCGTCCGCCG
TCTAGGTAAG
TTTAAAGCTC
AGGTCGAGAC
CGGGCCTTTG
TCCGGCGCTC
CCTTGGAGCC
TACCTAGACT
CAGCCGGCTC
TCCACGCTTT
GCCTGACCCT
GCTTGCTCAA
CTCTACGTCT
TTGTTTCGTT
TTCTGTTCTG
CGCCGTTACA
GATCCAAGCT
GTGACCGGCG
CCTACGCTAG
ACGCCACCAT
GGAGAGCGAC
GAGAGCGGCC
TGCCCGCCAT
GGAGATCGAG
TGCCGCATCA
CCGGCACCCT
GAACGGCGTG
GAGTTCGAGC
TGGTGGGCGG
CGGAGAGGGC
ACCCCCAAGC
AGGGCCGCAT
GACCAACAAG
ATGAAGAGCA
CCAAAGGCGC
CCTGACCTTC
AGCCCCTACC
TGCTGAGCCA
CGTGATGGGC
TACGGCTTCT
ACCACTTCGG
CACCTACCCC
AGCGGCTACG
AGAACCCCTT
CCTGCACGCC
ATCAACAACG
GCGGCTACAC
CAACACCCGC
ATCGAGAAGT
ACGAGGACGG
CGGCGTGCTG
CACGTGAGCT
TCAGCTACCG
CTACGAGGCC
GGCCGCGTGA
TCGGCGACTT
CAAGGTGGTG
GGCACCGGCT
TCCCCGAGGA
CAGCGTGATC
TTCACCGACA
AGATCATCCG
CAGCAACGCC
ACCGTGGAGC
ACCTGCACCC
CATGGGCGAT
AACGTGCTGG
TGGGCAGCTT
CGCCCGCACC
TTCAGCCTGC
GCGACGGCGG
CTACTACAGC
TTCGTGGTGG
ACAGCCACAT
GCACTTCAAG
AGCGCCATCC
ACCCCAGCAT
CCTGCAGAAC
GGGGGCCCCA
TGTTCGCCTT
CCGCCGCGTG
GAGGAGCTGC
ACAGCAACAC
CGAGCTGGGC
ATCGTGGAGT
ACCAGCACGC
CTTCAAGACC
CCCATCGCCT
TCGCCAGATC
CCGCGCTCAG
TCGTCCAATT
CTGCCGTGGA
CGGCACCGCC
GGACCCGGCT
CCACCGGATC
TCGCGAGGGC
AGAGGAAGTC
TTCTAACATG
CGGTGACGTG
GAGGAGAATC
CCGGCCCTAT
GACCGAGTAC
AAGCCCACGG
TGCGCCTCGC
CACCCGCGAC
GACGTCCCCA
GGGCCGTACG
CACCCTCGCC
GCCGCGTTCG
CCGACTACCC
CGCCACGCGC
CACACCGTCG
ATCCGGACCG
CCACATCGAG
CGGGTCACCG
AGCTGCAAGA
ACTCTTCCTC
ACGCGCGTCG
GGCTCGACAT
CGGCAAGGTG
TGGGTCGCGG
ACGACGGCGC
CGCGGTGGCG
GTCTGGACCA
CGCCGGAGAG
CGTCGAAGCG
GGGGCGGTGT
TCGCCGAGAT
CGGCCCGCGC
ATGGCCGAGT
TGAGCGGTTC
CCGGCTGGCC
GCGCAGCAAC
AGATGGAAGG
CCTCCTGGCG
CCGCACCGGC
CCAAGGAGCC
CGCGTGGTTC
CTGGCCACCG
TCGGCGTCTC
GCCCGACCAC
CAGGGCAAGG
GTCTGGGCAG
CGCCGTCGTG
CTCCCCGGAG
TGGAGGCGGC
CGAGCGCGCC
GGGGTGCCCG
CCTTCCTGGA
GACCTCCGCG
CCCCGCAACC
TCCCCTTCTA
CGAGCGGCTC
GGCTTCACCG
TCACCGCCGA
CGTCGAGGTG
CCCGAAGGAC
CGCGCACCTG
GTGCATGACC
CGCAAGCCCG
GTGCCTGAAA
TCAACCTCTG
GATTACAAAA
TTTGTGAAAG
ATTGACTGGT
ATTCTTAACT
ATGTTGCTCC
TTTTACGCTA
TGTGGATACG
CTGCTTTAAT
GCCTTTGTAT
CATGCTATTG
CTTCCCGTAT
GGCTTTCATT
TTCTCCTCCT
TGTATAAATC
CTGGTTGCTG
TCTCTTTATG
AGGAGTTGTG
GCCCGTTGTC
AGGCAACGTG
GCGTGGTGTG
CACTGTGTTT
GCTGACGCAA
CCCCCACTGG
TTGGGGCATT
GCCACCACCT
GTCAGCTCCT
TTCCGGGACT
TTCGCTTTCC
CCCTCCCTAT
TGCCACGGCG
GAACTCATCG
CCGCCTGCCT
TGCCCGCTGC
TGGACAGGGG
CTCGGCTGTT
GGGCACTGAC
AATTCCGTGG
TGTTGTCGGG
GAAGCTGACG
TCCTTTCCAT
GGCTGCTCGC
CTGTGTTGCC
ACCTGGATTC
TGCGCGGGAC
GTCCTTCTGC
TACGTCCCTT
CGGCCCTCAA
TCCAGCGGAC
CTTCCTTCCC
GCGGCCTGCT
GCCGGCTCTG
CGGCCTCTTC
CGCGTCTCCG
CCTTCGCCCT
CAGACGAGTC
GGATCTCCCT
TTGGCCGCCT
CCCCGCCTGG
TACCTTTAAG
ACCAATGACT
TACAAGGCAG
CTGTAGATCT
TAGCCACTTT
TTAAAAGAAA
AGGGGGGACT
GGAAGGGCTA
ATTCACTCCC
AACGAAAATA
AGATCTGCTT
TTTGCTTGTA
CTGGGTCTCT
CTGGTTAGAC
CAGATCTGAG
CCTGGGAGCT
CTCTGGCTAA
CTAGGGAACC
CACTGCTTAA
GCCTCAATAA
AGCTTGCCTT
GAGTGCTTCA
AGTAGTGTGT
GCCCGTCTGT
TGTGTGACTC
TGGTAACTAG
AGATCCCTCA
GACCCTTTTA
GTCAGTGTGG
AAAATCTCTA
GCAGTAGTAG
TTCATGTCAT
CTTATTATTC
AGTATTTATA
ACTTGCAAAG
AAATGAATAT
CAGAGAGTGA
GAGGAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGGCTCT
AGCTATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCGGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGACTTTTG
CAGAGACGGC
CCAAATTCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
GACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACGTCTAAGA
AACCATTATT
ATCATGACAT
TAACCTATAA
AAATAGGCGT
ATCACGAGGC
CCTTTCGTCT
CGCGCGTTTC
GGTGATGACG
GTGAAAACCT
CTGACACATG
CAGCTCCCGG
AGACGGTCAC
AGCTTGTCTG
TAAGCGGATG
CCGGGAGCAG
ACAAGCCCGT
CAGGGCGCGT
CAGCGGGTGT
TGGCGGGTGT
CGGGGCTGGC
TTAACTATGC
GGCATCAGAG
CAGATTGTAC
TGAGAGTGCA
CCATATGCGG
TGTGAAATAC
CGCACAGATG
CGTAAGGAGA
AAATACCGCA
TCAGGCGCCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGCTG
GCGAAAGGGG
GATGTGCTGC
AAGGCGATTA
AGTTGGGTAA
CGCCAGGGTT
TTCCCAGTCA
CGACGTTGTA
AAACGACGGC
CAGTGCCAAG
CTG