收藏网址 | 中文

pCMV-Tmem232(mouse)-EGFP-Neo

产品货号:P45651

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pCMV-Tmem232(mouse)-EGFP-Neo
别称: NM_001289485.1;E130009J12Rik; Gm940; Tes13-L; Tes13-S
基因长: 2028bp
质粒宿主: 哺乳细胞
质粒用途: 蛋白表达
片段类型: ORF
片段物种: 小鼠
原核抗性: Kan
筛选标记: Neo/G418
荧光标记: 绿色
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

TAGTTATTAA

TAGTAATCAA

TTACGGGGTC

ATTAGTTCAT

AGCCCATATA

TGGAGTTCCG

61

CGTTACATAA

CTTACGGTAA

ATGGCCCGCC

TGGCTGACCG

CCCAACGACC

CCCGCCCATT

121

GACGTCAATA

ATGACGTATG

TTCCCATAGT

AACGCCAATA

GGGACTTTCC

ATTGACGTCA

181

ATGGGTGGAG

TATTTACGGT

AAACTGCCCA

CTTGGCAGTA

CATCAAGTGT

ATCATATGCC

241

AAGTACGCCC

CCTATTGACG

TCAATGACGG

TAAATGGCCC

GCCTGGCATT

ATGCCCAGTA

301

CATGACCTTA

TGGGACTTTC

CTACTTGGCA

GTACATCTAC

GTATTAGTCA

TCGCTATTAC

361

CATGGTGATG

CGGTTTTGGC

AGTACATCAA

TGGGCGTGGA

TAGCGGTTTG

ACTCACGGGG

421

ATTTCCAAGT

CTCCACCCCA

TTGACGTCAA

TGGGAGTTTG

TTTTGGCACC

AAAATCAACG

481

GGACTTTCCA

AAATGTCGTA

ACAACTCCGC

CCCATTGACG

CAAATGGGCG

GTAGGCGTGT

541

ACGGTGGGAG

GTCTATATAA

GCAGAGCTGG

TTTAGTGAAC

CGTCAGATCC

GCTAGCGCTA

601

CCGGACTCAG

ATCTCGAGCT

CAAGCTTCGA

ATTCGCCACC

ATGAAAGCTT

ATAAGCCACC

661

TGTGGTCAAT

AAGTTTGGAG

TAATATCTTC

CACTTATCAT

GAAGAACTCC

TGAAATCTAT

721

TTTTGAAAGT

TCAAACAGAA

GAAAGAGTCA

AAAACCAAAG

CCATCATTTT

CAATTTCCAA

781

AGAATTCATA

TTGAGATTCA

ACCACACAGA

TAATCCAGCA

GAGGAGGAAG

AACTGTTGGA

841

ACAAGCCAGG

AGACTCATCG

TCAGGTCTAA

GAGGAAGTTG

GGCCTCAAGA

CACTGGGCTC

901

TGGGAAGCAT

GTGCATCTTC

CGACCGCATG

GGCTGAGGTC

ATCTATCTGG

CTCAGTGCAA

961

AGGGGAGATC

CAAGATGAAG

CTTTAAATAT

GCTCCACGCC

TCCTTGGACC

ATGTCTCCTT

1021

TGATCACGAT

CAGCTTCCTG

CCCTGTTTTT

TCTCGCGGAA

TCCGTTTTAT

ATCGACTCTG

1081

TTGCGATGCA

TTCATGAAGG

GATATTTATA

TTCAGTTGAG

ATAAAACTGG

TGAAGATTGG

1141

CTATTTGATC

TTCTTACGGC

TTTTTGTATT

CTTCCTGCAT

GGTCACTTAG

AAAGCTTTAA

1201

ACAACATTTG

CTTAGGCTGC

AGCCATATTT

ATACGCACTT

CATTTCTCGG

AACCGTCCTA

1261

CTACAAGTAT

CCGAACATCA

TCTCCAACGT

GCAGTTCATC

CTGAAAACAT

CGGAAATTAT

1321

CTGTAAAAGA

GAGCTTCATT

CTGAACCATT

CGTGGAAAGC

CCAGATGAAA

CTGAGGATCC

1381

ATATTCTGAC

CTGAATCATT

TGCAGCTGAA

TAAGAGGGGC

TACGAAGTCA

ACCACCTGCT

1441

CTGGCACAGT

GTGGCCGCCT

GGTCCTGCGT

TCAGAACAAT

CGTCCTCAGC

TCACCGAAGT

1501

GCTGGAACAC

CTCCTCTTCT

ACAAAACGCA

GCTTCAGACG

AAATGCTGGT

TGGATTCAGC

1561

CCTGGCTTTG

ATGGTCCTGG

GGGAAGCTGC

TAAGCTGGAC

ATGGCCTGCT

TGAAAACACT

1621

GATGGACCTA

GTGACAGATT

TCCTTGAGAA

CATCTTGTCT

GCTCAGAACC

AGGAAGAGAA

1681

CTATAATATA

TATGATACTT

CCTGGGCCTC

AGAGATTGTA

TTCACATATA

CAACAATTAT

1741

TGCAGAAGTC

TGTCTGTATG

CAGCTACCTC

TGACCTGAGG

AAAACAGCTT

TGATTGGGTT

1801

CTGTGCCTGT

AAAAGTCCGC

AGCAAGGTAT

TTCCCTCACA

GACAAGTCTG

AAGAGCTGCC

1861

AGAGTTGGAT

GGCGCAAGCA

TTTTAACTCT

TCTGAAGTAT

TTCTCTTCCA

GAATCTCTGA

1921

TAACTGTGAG

AAGGTGATCT

GGATTGGATA

CTATGGCATA

GTGTATAACC

TGGTGAAGAT

1981

GTCATGGGAA

CTTCAGGGTG

AGCAGGATCA

GGATGGACTT

AGAAACATGA

TATGGCAAAC

2041

ACTGCAAAAG

ATAAAGGATT

ATGAGCAAGA

CCCACGGATC

CGATGTGCAT

TAGTTATAGC

2101

CCAGGCCGAA

TTAAATGGCC

CATCTGACCC

TTTCTGCACA

AAGGCCACAC

CAAACTCAGG

2161

AGAAGAAGTT

TTCTCCAAGT

ACATCGGGTG

GAGAATCGCT

ACTACTCTCT

CCAGACTCTT

2221

TTTCCCCTCT

CTCGATGTTG

CCCCTCCAAA

GACGCCAGTG

GAAGTGGACC

TGCCAAGAAA

2281

ACACACCATC

AGAGAACGCC

AGCCTGCAAA

AAAGAGAGTT

CTGCGCTTTA

TCTTGAAGGA

2341

TCATTCTTCT

GTGGTAGAAG

TTTCCATGAC

TCCGTATCCA

AATTTCTTCA

CCAAGGCAGA

2401

TAAGAAATTG

GAAGAAATCA

TTGATCATCA

TTGGCAGAAA

GATATGGAGG

CCCGAAAGCG

2461

AGAAGAGGAA

GCATATAAGG

CCCAAAATCA

GAAGGACAAG

GAGGAAAAGG

AGAAGATTCA

2521

CTTTCAAGAA

ATTATGAAGC

AAAGAGAACG

GAAACTGAAC

AAACAAACCA

AACCCTATGA

2581

AATAATTCTG

TCAGAAAAAG

AAAGTGGCTC

AGAAAAGAAA

TGTGGGTTCT

TCGAGCTGAA

2641

ACCCAGCACC

GCACCAAATG

CTAAGGGCGG

TGGAGGTTCA

GGCGGTGGAG

GATCTCAGGA

2701

TCCACCGGTC

GCCACCATGG

TGAGCAAGGG

CGAGGAGCTG

TTCACCGGGG

TGGTGCCCAT

2761

CCTGGTCGAG

CTGGACGGCG

ACGTAAACGG

CCACAAGTTC

AGCGTGTCCG

GCGAGGGCGA

2821

GGGCGATGCC

ACCTACGGCA

AGCTGACCCT

GAAGTTCATC

TGCACCACCG

GCAAGCTGCC

2881

CGTGCCCTGG

CCCACCCTCG

TGACCACCCT

GACCTACGGC

GTGCAGTGCT

TCAGCCGCTA

2941

CCCCGACCAC

ATGAAGCAGC

ACGACTTCTT

CAAGTCCGCC

ATGCCCGAAG

GCTACGTCCA

3001

GGAGCGCACC

ATCTTCTTCA

AGGACGACGG

CAACTACAAG

ACCCGCGCCG

AGGTGAAGTT

3061

CGAGGGCGAC

ACCCTGGTGA

ACCGCATCGA

GCTGAAGGGC

ATCGACTTCA

AGGAGGACGG

3121

CAACATCCTG

GGGCACAAGC

TGGAGTACAA

CTACAACAGC

CACAACGTCT

ATATCATGGC

3181

CGACAAGCAG

AAGAACGGCA

TCAAGGTGAA

CTTCAAGATC

CGCCACAACA

TCGAGGACGG

3241

CAGCGTGCAG

CTCGCCGACC

ACTACCAGCA

GAACACCCCC

ATCGGCGACG

GCCCCGTGCT

3301

GCTGCCCGAC

AACCACTACC

TGAGCACCCA

GTCCGCCCTG

AGCAAAGACC

CCAACGAGAA

3361

GCGCGATCAC

ATGGTCCTGC

TGGAGTTCGT

GACCGCCGCC

GGGATCACTC

TCGGCATGGA

3421

CGAGCTGTAC

AAGTAAAGCG

GCCGCGACTC

TAGATCATAA

TCAGCCATAC

CACATTTGTA

3481

GAGGTTTTAC

TTGCTTTAAA

AAACCTCCCA

CACCTCCCCC

TGAACCTGAA

ACATAAAATG

3541

AATGCAATTG

TTGTTGTTAA

CTTGTTTATT

GCAGCTTATA

ATGGTTACAA

ATAAAGCAAT

3601

AGCATCACAA

ATTTCACAAA

TAAAGCATTT

TTTTCACTGC

ATTCTAGTTG

TGGTTTGTCC

3661

AAACTCATCA

ATGTATCTTA

AGGCGTAAAT

TGTAAGCGTT

AATATTTTGT

TAAAATTCGC

3721

GTTAAATTTT

TGTTAAATCA

GCTCATTTTT

TAACCAATAG

GCCGAAATCG

GCAAAATCCC

3781

TTATAAATCA

AAAGAATAGA

CCGAGATAGG

GTTGAGTGTT

GTTCCAGTTT

GGAACAAGAG

3841

TCCACTATTA

AAGAACGTGG

ACTCCAACGT

CAAAGGGCGA

AAAACCGTCT

ATCAGGGCGA

3901

TGGCCCACTA

CGTGAACCAT

CACCCTAATC

AAGTTTTTTG

GGGTCGAGGT

GCCGTAAAGC

3961

ACTAAATCGG

AACCCTAAAG

GGAGCCCCCG

ATTTAGAGCT

TGACGGGGAA

AGCCGGCGAA

4021

CGTGGCGAGA

AAGGAAGGGA

AGAAAGCGAA

AGGAGCGGGC

GCTAGGGCGC

TGGCAAGTGT

4081

AGCGGTCACG

CTGCGCGTAA

CCACCACACC

CGCCGCGCTT

AATGCGCCGC

TACAGGGCGC

4141

GTCAGGTGGC

ACTTTTCGGG

GAAATGTGCG

CGGAACCCCT

ATTTGTTTAT

TTTTCTAAAT

4201

ACATTCAAAT

ATGTATCCGC

TCATGAGACA

ATAACCCTGA

TAAATGCTTC

AATAATATTG

4261

AAAAAGGAAG

AGTCCTGAGG

CGGAAAGAAC

CAGCTGTGGA

ATGTGTGTCA

GTTAGGGTGT

4321

GGAAAGTCCC

CAGGCTCCCC

AGCAGGCAGA

AGTATGCAAA

GCATGCATCT

CAATTAGTCA

4381

GCAACCAGGT

GTGGAAAGTC

CCCAGGCTCC

CCAGCAGGCA

GAAGTATGCA

AAGCATGCAT

4441

CTCAATTAGT

CAGCAACCAT

AGTCCCGCCC

CTAACTCCGC

CCATCCCGCC

CCTAACTCCG

4501

CCCAGTTCCG

CCCATTCTCC

GCCCCATGGC

TGACTAATTT

TTTTTATTTA

TGCAGAGGCC

4561

GAGGCCGCCT

CGGCCTCTGA

GCTATTCCAG

AAGTAGTGAG

GAGGCTTTTT

TGGAGGCCTA

4621

GGCTTTTGCA

AAGATCGATC

AAGAGACAGG

ATGAGGATCG

TTTCGCATGA

TTGAACAAGA

4681

TGGATTGCAC

GCAGGTTCTC

CGGCCGCTTG

GGTGGAGAGG

CTATTCGGCT

ATGACTGGGC

4741

ACAACAGACA

ATCGGCTGCT

CTGATGCCGC

CGTGTTCCGG

CTGTCAGCGC

AGGGGCGCCC

4801

GGTTCTTTTT

GTCAAGACCG

ACCTGTCCGG

TGCCCTGAAT

GAACTGCAAG

ACGAGGCAGC

4861

GCGGCTATCG

TGGCTGGCCA

CGACGGGCGT

TCCTTGCGCA

GCTGTGCTCG

ACGTTGTCAC

4921

TGAAGCGGGA

AGGGACTGGC

TGCTATTGGG

CGAAGTGCCG

GGGCAGGATC

TCCTGTCATC

4981

TCACCTTGCT

CCTGCCGAGA

AAGTATCCAT

CATGGCTGAT

GCAATGCGGC

GGCTGCATAC

5041

GCTTGATCCG

GCTACCTGCC

CATTCGACCA

CCAAGCGAAA

CATCGCATCG

AGCGAGCACG

5101

TACTCGGATG

GAAGCCGGTC

TTGTCGATCA

GGATGATCTG

GACGAAGAGC

ATCAGGGGCT

5161

CGCGCCAGCC

GAACTGTTCG

CCAGGCTCAA

GGCGAGCATG

CCCGACGGCG

AGGATCTCGT

5221

CGTGACCCAT

GGCGATGCCT

GCTTGCCGAA

TATCATGGTG

GAAAATGGCC

GCTTTTCTGG

5281

ATTCATCGAC

TGTGGCCGGC

TGGGTGTGGC

GGACCGCTAT

CAGGACATAG

CGTTGGCTAC

5341

CCGTGATATT

GCTGAAGAGC

TTGGCGGCGA

ATGGGCTGAC

CGCTTCCTCG

TGCTTTACGG

5401

TATCGCCGCT

CCCGATTCGC

AGCGCATCGC

CTTCTATCGC

CTTCTTGACG

AGTTCTTCTG

5461

AGCGGGACTC

TGGGGTTCGA

AATGACCGAC

CAAGCGACGC

CCAACCTGCC

ATCACGAGAT

5521

TTCGATTCCA

CCGCCGCCTT

CTATGAAAGG

TTGGGCTTCG

GAATCGTTTT

CCGGGACGCC

5581

GGCTGGATGA

TCCTCCAGCG

CGGGGATCTC

ATGCTGGAGT

TCTTCGCCCA

CCCTAGGGGG

5641

AGGCTAACTG

AAACACGGAA

GGAGACAATA

CCGGAAGGAA

CCCGCGCTAT

GACGGCAATA

5701

AAAAGACAGA

ATAAAACGCA

CGGTGTTGGG

TCGTTTGTTC

ATAAACGCGG

GGTTCGGTCC

5761

CAGGGCTGGC

ACTCTGTCGA

TACCCCACCG

AGACCCCATT

GGGGCCAATA

CGCCCGCGTT

5821

TCTTCCTTTT

CCCCACCCCA

CCCCCCAAGT

TCGGGTGAAG

GCCCAGGGCT

CGCAGCCAAC

5881

GTCGGGGCGG

CAGGCCCTGC

CATAGCCTCA

GGTTACTCAT

ATATACTTTA

GATTGATTTA

5941

AAACTTCATT

TTTAATTTAA

AAGGATCTAG

GTGAAGATCC

TTTTTGATAA

TCTCATGACC

6001

AAAATCCCTT

AACGTGAGTT

TTCGTTCCAC

TGAGCGTCAG

ACCCCGTAGA

AAAGATCAAA

6061

GGATCTTCTT

GAGATCCTTT

TTTTCTGCGC

GTAATCTGCT

GCTTGCAAAC

AAAAAAACCA

6121

CCGCTACCAG

CGGTGGTTTG

TTTGCCGGAT

CAAGAGCTAC

CAACTCTTTT

TCCGAAGGTA

6181

ACTGGCTTCA

GCAGAGCGCA

GATACCAAAT

ACTGTTCTTC

TAGTGTAGCC

GTAGTTAGGC

6241

CACCACTTCA

AGAACTCTGT

AGCACCGCCT

ACATACCTCG

CTCTGCTAAT

CCTGTTACCA

6301

GTGGCTGCTG

CCAGTGGCGA

TAAGTCGTGT

CTTACCGGGT

TGGACTCAAG

ACGATAGTTA

6361

CCGGATAAGG

CGCAGCGGTC

GGGCTGAACG

GGGGGTTCGT

GCACACAGCC

CAGCTTGGAG

6421

CGAACGACCT

ACACCGAACT

GAGATACCTA

CAGCGTGAGC

TATGAGAAAG

CGCCACGCTT

6481

CCCGAAGGGA

GAAAGGCGGA

CAGGTATCCG

GTAAGCGGCA

GGGTCGGAAC

AGGAGAGCGC

6541

ACGAGGGAGC

TTCCAGGGGG

AAACGCCTGG

TATCTTTATA

GTCCTGTCGG

GTTTCGCCAC

6601

CTCTGACTTG

AGCGTCGATT

TTTGTGATGC

TCGTCAGGGG

GGCGGAGCCT

ATGGAAAAAC

6661

GCCAGCAACG

CGGCCTTTTT

ACGGTTCCTG

GCCTTTTGCT

GGCCTTTTGC

TCACATGTTC

6721

TTTCCTGCGT

TATCCCCTGA

TTCTGTGGAT

AACCGTATTA

CCGCCATGCA

T

武汉网监电子标识 Copyright © 武汉淼灵生物科技有限公司版权所有/鄂公网安备42010702000282号 鄂ICP备14012402号-1
访客数:2972992
1.287638s