产品货号：P45553
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV2-CMV-EGFP-MYLK(human)(1同义突变)-Puro |
|---|---|
| 别称: | NM_053025.4(1同义突变);AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK |
| 基因长: | 5745bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | 绿色 |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ATTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCGGGTT
TATTACAGGG
ACAGCAGAGA
TCCAGTTTAT
CGATAAGCTT
GGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCG
TTTAGTGAAC
CGTCAGATCG
CCTGGAGACG
CCATCCACGC
TGTTTTGACC
TCCATAGAAG
ACACCGACTC
TACTAGAGGA
TCGCTAGCGC
TACCGGACTC
AGATCTCGAG
CTCAAGCTTC
GAATTCGCCA
CCATGGTGAG
CAAGGGCGAG
GAGCTGTTCA
CCGGGGTGGT
GCCCATCCTG
GTCGAGCTGG
ACGGCGACGT
AAACGGCCAC
AAGTTCAGCG
TGTCCGGCGA
GGGCGAGGGC
GATGCCACCT
ACGGCAAGCT
GACCCTGAAG
TTCATCTGCA
CCACCGGCAA
GCTGCCCGTG
CCCTGGCCCA
CCCTCGTGAC
CACCCTGACC
TACGGCGTGC
AGTGCTTCAG
CCGCTACCCC
GACCACATGA
AGCAGCACGA
CTTCTTCAAG
TCCGCCATGC
CCGAAGGCTA
CGTCCAGGAG
CGCACCATCT
TCTTCAAGGA
CGACGGCAAC
TACAAGACCC
GCGCCGAGGT
GAAGTTCGAG
GGCGACACCC
TGGTGAACCG
CATCGAGCTG
AAGGGCATCG
ACTTCAAGGA
GGACGGCAAC
ATCCTGGGGC
ACAAGCTGGA
GTACAACTAC
AACAGCCACA
ACGTCTATAT
CATGGCCGAC
AAGCAGAAGA
ACGGCATCAA
GGTGAACTTC
AAGATCCGCC
ACAACATCGA
GGACGGCAGC
GTGCAGCTCG
CCGACCACTA
CCAGCAGAAC
ACCCCCATCG
GCGACGGCCC
CGTGCTGCTG
CCCGACAACC
ACTACCTGAG
CACCCAGTCC
GCCCTGAGCA
AAGACCCCAA
CGAGAAGCGC
GATCACATGG
TCCTGCTGGA
GTTCGTGACC
GCCGCCGGGA
TCACTCTCGG
CATGGACGAG
CTGTACAAGT
CCGGACTCAG
ATCTCGAGGC
GGCGGAGGGA
GTGGAGGAGG
GGGAAGCGGA
GGCGGAGGGA
GTGGATCCAT
GGGGGATGTG
AAGCTGGTTG
CCTCGTCACA
CATTTCCAAA
ACCTCCCTCA
GTGTGGATCC
CTCAAGAGTT
GACTCCATGC
CCCTGACAGA
GGCCCCTGCT
TTCATTTTGC
CCCCTCGGAA
CCTCTGCATC
AAAGAAGGAG
CCACCGCCAA
GTTCGAAGGG
CGGGTCCGGG
GTTACCCAGA
GCCCCAGGTG
ACATGGCACA
GAAACGGGCA
ACCCATCACC
AGCGGGGGCC
GCTTCCTGCT
GGATTGCGGC
ATCCGGGGGA
CTTTCAGCCT
TGTGATTCAT
GCTGTCCATG
AGGAGGACAG
GGGAAAGTAT
ACCTGTGAAG
CCACCAATGG
CAGTGGTGCT
CGCCAGGTGA
CAGTGGAGTT
GACAGTAGAA
GGAAGTTTTG
CGAAGCAGCT
TGGTCAGCCT
GTTGTTTCCA
AAACCTTAGG
GGATAGATTT
TCAGCTCCAG
CAGTGGAGAC
CCGTCCTAGC
ATCTGGGGGG
AGTGCCCACC
AAAGTTTGCT
ACCAAGCTGG
GCCGAGTTGT
GGTCAAAGAA
GGACAGATGG
GACGATTCTC
CTGCAAGATC
ACTGGCCGGC
CCCAACCGCA
GGTCACCTGG
CTCAAGGGAA
ATGTTCCACT
GCAGCCGAGT
GCCCGTGTGT
CTGTGTCTGA
GAAGAACGGC
ATGCAGGTTC
TGGAAATCCA
TGGAGTCAAC
CAAGATGACG
TGGGAGTGTA
CACGTGCCTG
GTGGTGAACG
GGTCGGGGAA
GGCCTCGATG
TCAGCTGAAC
TTTCCATCCA
AGGTTTGGAC
AGTGCCAATA
GGTCATTTGT
GAGAGAAACA
AAAGCCACCA
ATTCAGATGT
CAGGAAAGAG
GTGACCAATG
TAATCTCAAA
GGAGTCGAAG
CTGGACAGTC
TGGAGGCTGC
AGCCAAAAGC
AAGAACTGCT
CCAGCCCCCA
GAGAGGTGGC
TCCCCACCCT
GGGCTGCAAA
CAGCCAGCCT
CAGCCCCCAA
GGGAGTCCAA
GCTGGAGTCA
TGCAAGGACT
CGCCCAGAAC
GGCCCCGCAG
ACCCCGGTCC
TTCAGAAGAC
TTCCAGCTCC
ATCACCCTGC
AGGCCGCAAG
AGTTCAGCCG
GAACCAAGAG
CACCAGGCCT
GGGGGTCCTA
TCACCTTCTG
GAGAAGAGAG
GAAGAGGCCA
GCTCCTCCCC
GTCCAGCCAC
CTTCCCCACC
AGGCAGCCTG
GCCTGGGGAG
CCAAGATGTT
GTGAGCAAGG
CTGCTAACAG
GAGAATCCCC
ATGGAGGGCC
AGAGGGATTC
AGCATTCCCC
AAATTTGAGA
GCAAGCCCCA
AAGCCAGGAG
GTCAAGGAAA
ATCAAACTGT
CAAGTTCAGA
TGTGAAGTTT
CCGGGATTCC
AAAGCCTGAA
GTGGCCTGGT
TCCTGGAAGG
CACCCCCGTG
AGGAGACAGG
AAGGCAGCAT
TGAGGTTTAT
GAAGATGCTG
GCTCCCATTA
CCTCTGCCTG
CTGAAAGCCC
GGACCAGGGA
CAGTGGGACA
TACAGCTGCA
CTGCTTCCAA
CGCCCAAGGC
CAGCTGTCCT
GTAGCTGGAC
CCTCCAAGTG
GAAAGGCTTG
CCGTGATGGA
GGTGGCCCCC
TCCTTCTCCA
GTGTCCTGAA
GGACTGCGCT
GTTATTGAGG
GCCAGGATTT
TGTGCTGCAG
TGCTCCGTAC
GGGGGACCCC
AGTGCCCCGG
ATCACTTGGC
TGCTGAATGG
GCAGCCCATC
CAGTACGCTC
GCTCCACCTG
CGAGGCCGGC
GTGGCTGAGC
TCCACATCCA
GGATGCCCTG
CCGGAGGACC
ATGGCACCTA
CACCTGCCTA
GCTGAGAATG
CCTTGGGGCA
GGTGTCCTGC
AGCGCCTGGG
TCACCGTCCA
TGAAAAGAAG
AGTAGCAGGA
AGAGTGAGTA
CCTTCTGCCT
GTGGCTCCCA
GCAAGCCCAC
TGCACCCATC
TTCCTGCAGG
GCCTCTCTGA
TCTCAAAGTC
ATGGATGGAA
GCCAGGTCAC
TATGACTGTC
CAAGTGTCAG
GGAATCCACC
CCCTGAAGTC
ATCTGGCTGC
ACAATGGGAA
TGAGATCCAA
GAGTCAGAGG
ACTTCCACTT
TGAACAGAGA
GGAACTCAGC
ACAGCCTTTG
TATCCAGGAA
GTGTTCCCGG
AGGACACGGG
CACGTACACC
TGCGAGGCCT
GGAACAGCGC
TGGAGAGGTC
CGCACCCAGG
CCGTGCTCAC
GGTACAAGAG
CCTCACGATG
GCACCCAGCC
CTGGTTCATC
AGTAAGCCTC
GCTCAGTGAC
AGCCTCCCTG
GGCCAGAGTG
TCCTCATCTC
CTGCGCCATA
GCTGGTGACC
CCTTTCCTAC
CGTGCACTGG
CTCAGAGATG
GCAAAGCCCT
CTGCAAAGAC
ACTGGCCACT
TCGAGGTGCT
TCAGAATGAG
GACGTGTTCA
CCCTGGTTCT
AAAGAAGGTG
CAGCCCTGGC
ATGCCGGCCA
GTATGAGATC
CTGCTCAAGA
ACCGGGTTGG
CGAATGCAGT
TGCCAGGTGT
CACTGATGCT
ACAGAACAGC
TCTGCCAGAG
CCCTTCCACG
GGGGAGGGAG
CCTGCCAGCT
GCGAGGACCT
CTGTGGTGGA
GGAGTTGGTG
CTGATGGTGG
TGGTAGTGAC
CGCTATGGGT
CCCTGAGGCC
TGGCTGGCCA
GCAAGAGGGC
AGGGTTGGCT
AGAGGAGGAA
GACGGCGAGG
ACGTGCGAGG
GGTGCTGAAG
AGGCGCGTGG
AGACGAGGCA
GCACACTGAG
GAGGCGATCC
GCCAGCAGGA
GGTGGAGCAG
CTGGACTTCC
GAGACCTCCT
GGGGAAGAAG
GTGAGTACAA
AGACCCTATC
GGAAGACGAC
CTGAAGGAGA
TCCCAGCCGA
GCAGATGGAT
TTCCGTGCCA
ACCTGCAGCG
GCAAGTGAAG
CCAAAGACTG
TGTCTGAGGA
AGAGAGGAAG
GTGCACAGCC
CCCAGCAGGT
CGATTTTCGC
TCTGTCCTGG
CCAAGAAGGG
GACTTCCAAG
ACCCCCGTGC
CTGAGAAGGT
GCCACCGCCA
AAACCTGCCA
CCCCGGATTT
TCGCTCAGTG
CTGGGTGGCA
AGAAGAAATT
ACCAGCAGAG
AATGGCAGCA
GCAGTGCCGA
GACCCTGAAT
GCCAAGGCAG
TGGAGAGTTC
CAAGCCCCTG
AGCAATGCAC
AGCCTTCAGG
GCCCTTGAAA
CCCGTGGGCA
ACGCCAAGCC
TGCTGAGACC
CTGAAGCCAA
TGGGCAACGC
CAAGCCTGCC
GAGACCCTGA
AGCCCATGGG
CAATGCCAAG
CCTGATGAGA
ACCTGAAATC
CGCTAGCAAA
GAAGAACTCA
AGAAAGACGT
TAAGAATGAT
GTGAACTGCA
AGAGAGGCCA
TGCAGGGACC
ACAGATAATG
AAAAGAGATC
AGAGAGCCAG
GGGACAGCCC
CAGCCTTCAA
GCAGAAGCTG
CAAGATGTTC
ATGTGGCAGA
GGGCAAGAAG
CTGCTGCTCC
AGTGCCAGGT
GTCTTCTGAC
CCCCCAGCCA
CCATCATCTG
GACGCTGAAC
GGAAAGACCC
TCAAGACCAC
CAAGTTCATC
ATCCTCTCCC
AGGAAGGCTC
ACTCTGCTCC
GTCTCCATCG
AGAAGGCACT
GCCTGAGGAC
AGAGGCTTAT
ACAAGTGTGT
AGCCAAGAAT
GACGCTGGCC
AGGCGGAGTG
CTCCTGCCAA
GTCACCGTGG
ATGATGCTCC
AGCCAGTGAG
AACACCAAGG
CCCCAGAGAT
GAAATCCCGG
AGGCCCAAGA
GCTCTCTTCC
TCCCGTGCTA
GGAACTGAGA
GTGATGCGAC
TGTGAAAAAG
AAACCTGCCC
CCAAGACACC
TCCGAAGGCA
GCAATGCCCC
CTCAGATCAT
CCAGTTCCCT
GAGGACCAGA
AGGTACGCGC
AGGAGAGTCA
GTGGAGCTGT
TTGGCAAAGT
GACAGGCACT
CAGCCCATCA
CCTGTACCTG
GATGAAGTTC
CGAAAGCAGA
TCCAGGAAAG
CGAGCACATG
AAGGTGGAGA
ACAGCGAGAA
TGGCAGCAAG
CTCACCATCC
TGGCCGCGCG
CCAGGAGCAC
TGCGGCTGCT
ACACACTGCT
GGTGGAGAAC
AAGCTGGGCA
GCAGGCAGGC
CCAGGTCAAC
CTCACTGTCG
TGGATAAGCC
AGACCCCCCA
GCTGGCACAC
CTTGTGCCTC
TGACATTCGG
AGCTCCTCAC
TGACCCTGTC
CTGGTATGGC
TCCTCATATG
ATGGGGGCAG
TGCTGTACAG
TCCTACAGCA
TCGAGATCTG
GGACTCAGCC
AACAAGACGT
GGAAGGAACT
AGCCACATGC
CGCAGCACCT
CTTTCAACGT
CCAGGACCTG
CTGCCTGACC
ACGAATATAA
GTTCCGTGTA
CGTGCAATCA
ACGTGTATGG
AACCAGTGAG
CCAAGCCAGG
AGTCTGAACT
CACAACGGTA
GGAGAGAAAC
CTGAAGAGCC
GAAGGATGAA
GTGGAGGTGT
CAGATGATGA
TGAGAAGGAG
CCCGAGGTTG
ATTACCGGAC
AGTGACAATC
AATACTGAAC
AAAAAGTATC
TGACTTCTAC
GACATTGAGG
AGAGATTAGG
ATCTGGGAAA
TTTGGACAGG
TCTTTCGACT
TGTAGAAAAG
AAAACTCGAA
AAGTCTGGGC
AGGGAAGTTC
TTCAAGGCAT
ATTCAGCAAA
AGAGAAAGAG
AATATCCGGC
AGGAGATTAG
CATCATGAAC
TGCCTCCACC
ACCCTAAGCT
GGTCCAGTGT
GTGGATGCCT
TTGAAGAAAA
GGCCAACATC
GTCATGGTCC
TGGAGATCGT
GTCAGGAGGG
GAGCTGTTTG
AGCGCATCAT
TGACGAGGAC
TTTGAGCTGA
CGGAGCGTGA
GTGCATCAAG
TACATGCGGC
AGATCTCGGA
GGGAGTGGAG
TACATCCACA
AGCAGGGCAT
CGTGCACCTG
GACCTCAAGC
CGGAGAACAT
CATGTGTGTC
AACAAGACGG
GCACCAGGAT
CAAGCTCATC
GACTTTGGTC
TGGCCAGGAG
GCTGGAGAAT
GCGGGGTCTC
TGAAGGTCCT
CTTTGGCACC
CCAGAATTTG
TGGCTCCTGA
AGTGATCAAC
TATGAGCCCA
TCGGCTACGC
CACAGACATG
TGGAGCATCG
GGGTCATCTG
CTACATCCTA
GTCAGTGGCC
TTTCCCCCTT
CATGGGAGAC
AACGATAACG
AAACCTTGGC
CAACGTTACC
TCAGCCACCT
GGGACTTCGA
CGACGAGGCA
TTCGATGAGA
TCTCCGACGA
TGCCAAGGAT
TTCATCAGCA
ATCTGCTGAA
GAAAGATATG
AAAAACCGCC
TGGACTGCAC
GCAGTGCCTT
CAGCATCCAT
GGCTAATGAA
AGATACCAAG
AACATGGAGG
CCAAGAAACT
CTCCAAGGAC
CGGATGAAGA
AGTACATGGC
AAGAAGGAAA
TGGCAGAAAA
CGGGCAATGC
TGTGAGAGCC
ATTGGAAGAC
TGTCCTCTAT
GGCAATGATC
TCAGGGCTCA
GTGGCAGGAA
ATCCTCAACA
GGGTCACCAA
CCAGCCCGCT
CAATGCAGAA
AAACTAGAAT
CTGAAGAAGA
TGTGTCCCAA
GCTTTCCTTG
AGGCTGTTGC
TGAGGAAAAG
CCTCATGTAA
AACCCTATTT
CTCTAAGACC
ATTCGCGATT
TAGAAGTTGT
GGAGGGAAGT
GCTGCTAGAT
TTGACTGCAA
GATTGAAGGA
TACCCAGACC
CCGAGGTTGT
CTGGTTCAAA
GATGACCAGT
CAATCAGGGA
GTCCCGCCAC
TTCCAGATAG
ACTACGATGA
GGACGGGAAC
TGCTCTTTAA
TTATTAGTGA
TGTTTGCGGG
GATGACGATG
CCAAGTACAC
CTGCAAGGCT
GTCAACAGTC
TTGGAGAAGC
CACCTGCACA
GCAGAGCTCA
TTGTGGAAAC
GATGGAGGAA
GGTGAAGGGG
AAGGGGAAGA
GGAAGAAGAG
TGATCTAGAT
AATTCTACCG
GGTAGGGGAG
GCGCTTTTCC
CAAGGCAGTC
TGGAGCATGC
GCTTTAGCAG
CCCCGCTGGG
CACTTGGCGC
TACACAAGTG
GCCTCTGGCC
TCGCACACAT
TCCACATCCA
CCGGTAGGCG
CCAACCGGCT
CCGTTCTTTG
GTGGCCCCTT
CGCGCCACCT
TCTACTCCTC
CCCTAGTCAG
GAAGTTCCCC
CCCGCCCCGC
AGCTCGCGTC
GTGCAGGACG
TGACAAATGG
AAGTAGCACG
TCTCACTAGT
CTCGTGCAGA
TGGACAGCAC
CGCTGAGCAA
TGGAAGCGGG
TAGGCCTTTG
GGGCAGCGGC
CAATAGCAGC
TTTGCTCCTT
CGCTTTCTGG
GCTCAGAGGC
TGGGAAGGGG
TGGGTCCGGG
GGCGGGCTCA
GGGGCGGGCT
CAGGGGCGGG
GCGGGCGCCC
GAAGGTCCTC
CGGAGGCCCG
GCATTCTGCA
CGCTTCAAAA
GCGCACGTCT
GCCGCGCTGT
TCTCCTCTTC
CTCATCTCCG
GGCCTTTCGA
CCTGCAGCCC
AAGCTTACCA
TGACCGAGTA
CAAGCCCACG
GTGCGCCTCG
CCACCCGCGA
CGACGTCCCC
AGGGCCGTAC
GCACCCTCGC
CGCCGCGTTC
GCCGACTACC
CCGCCACGCG
CCACACCGTC
GATCCGGACC
GCCACATCGA
GCGGGTCACC
GAGCTGCAAG
AACTCTTCCT
CACGCGCGTC
GGGCTCGACA
TCGGCAAGGT
GTGGGTCGCG
GACGACGGCG
CCGCGGTGGC
GGTCTGGACC
ACGCCGGAGA
GCGTCGAAGC
GGGGGCGGTG
TTCGCCGAGA
TCGGCCCGCG
CATGGCCGAG
TTGAGCGGTT
CCCGGCTGGC
CGCGCAGCAA
CAGATGGAAG
GCCTCCTGGC
GCCGCACCGG
CCCAAGGAGC
CCGCGTGGTT
CCTGGCCACC
GTCGGCGTCT
CGCCCGACCA
CCAGGGCAAG
GGTCTGGGCA
GCGCCGTCGT
GCTCCCCGGA
GTGGAGGCGG
CCGAGCGCGC
CGGGGTGCCC
GCCTTCCTGG
AGACCTCCGC
GCCCCGCAAC
CTCCCCTTCT
ACGAGCGGCT
CGGCTTCACC
GTCACCGCCG
ACGTCGAGGT
GCCCGAAGGA
CCGCGCACCT
GGTGCATGAC
CCGCAAGCCC
GGTGCCTGAC
CGCGTCTGGA
ACAATCAACC
TCTGGATTAC
AAAATTTGTG
AAAGATTGAC
TGGTATTCTT
AACTATGTTG
CTCCTTTTAC
GCTATGTGGA
TACGCTGCTT
TAATGCCTTT
GTATCATGCT
ATTGCTTCCC
GTATGGCTTT
CATTTTCTCC
TCCTTGTATA
AATCCTGGTT
GCTGTCTCTT
TATGAGGAGT
TGTGGCCCGT
TGTCAGGCAA
CGTGGCGTGG
TGTGCACTGT
GTTTGCTGAC
GCAACCCCCA
CTGGTTGGGG
CATTGCCACC
ACCTGTCAGC
TCCTTTCCGG
GACTTTCGCT
TTCCCCCTCC
CTATTGCCAC
GGCGGAACTC
ATCGCCGCCT
GCCTTGCCCG
CTGCTGGACA
GGGGCTCGGC
TGTTGGGCAC
TGACAATTCC
GTGGTGTTGT
CGGGGAAGCT
GACGTCCTTT
CCATGGCTGC
TCGCCTGTGT
TGCCACCTGG
ATTCTGCGCG
GGACGTCCTT
CTGCTACGTC
CCTTCGGCCC
TCAATCCAGC
GGACCTTCCT
TCCCGCGGCC
TGCTGCCGGC
TCTGCGGCCT
CTTCCGCGTC
TTCGCCTTCG
CCCTCAGACG
AGTCGGATCT
CCCTTTGGGC
CGCCTCCCCG
CCTGGAATTA
ATTCTGCAGT
CGAGACCTAG
AAAAACATGG
AGCAATCACA
AGTAGCAATA
CAGCAGCTAC
CAATGCTGAT
TGTGCCTGGC
TAGAAGCACA
AGAGGAGGAG
GAGGTGGGTT
TTCCAGTCAC
ACCTCAGGTA
CCTTTAAGAC
CAATGACTTA
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
AGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGACAAG
ATATCCTTGA
TCTGTGGATC
TACCACACAC
AAGGCTACTT
CCCTGATTAG
CAGAACTACA
CACCAGGGCC
AGGGGTCAGA
TATCCACTGA
CCTTTGGATG
GTGCTACAAG
CTAGTACCAG
TTGAGCCAGA
TAAGGTAGAA
GAGGCCAATA
AAGGAGAGAA
CACCAGCTTG
TTACACCCTG
TGAGCCTGCA
TGGGATGGAT
GACCCGGAGA
GAGAAGTGTT
AGAGTGGAGG
TTTGACAGCC
GCCTAGCATT
TCATCACGTG
GCCCGAGAGC
TGCATCCGGA
GTACTTCAAG
AACTGCTGAT
ATCGAGCTTG
CTACAAGGGA
CTTTCCGCTG
GGGACTTTCC
AGGGAGGCGT
GGCCTGGGCG
GGACTGGGGA
GTGGCGAGCC
CTCAGATCCT
GCATATAAGC
AGCTGCTTTT
TGCCTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTAGTAGTT
CATGTCATCT
TATTATTCAG
TATTTATAAC
TTGCAAAGAA
ATGAATATCA
GAGAGTGAGA
GGCCTTGACA
TTGCTAGCGT
TTTACCGTCG
ACCTCTAGCT
AGAGCTTGGC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGACGTCGAC
GGATCGGGAG
ATCAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCATTCT
AGTTGTGGTT
TGTCCAAACT
CATCAATGTA
TCTTATCATG
TCTGGATCAA
CTGGATAACT
CAAGCTAACC
AAAATCATCC
CAAACTTCCC
ACCCCATACC
CTATTACCAC
TGCCAATTAC
CTGTGGTTTC
ATTTACTCTA
AACCTGTGAT
TCCTCTGAAT
TATTTTCATT
TTAAAGAAAT
TGTATTTGTT
AAATATGTAC
TACAAACTTA
GTAGTTTTTA
AAGAAATTGT
ATTTGTTAAA
TATGTACTAC
AAACTTAGTA
GT
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