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pIVT-dCas9-EGFP

产品货号:P45545

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pIVT-dCas9-EGFP
别称: Plasmid#174442
基因长:
质粒宿主: 大肠杆菌
质粒用途: 基因编辑
片段类型: CRISPR
片段物种:
原核抗性: Amp
筛选标记:
荧光标记: 绿色
启动子:
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

GACGAAAGGG

CCTCGTGATA

CGCCTATTTT

TATAGGTTAA

TGTCATGATA

ATAATGGTTT

61

CTTAGACGTC

AGGTGGCACT

TTTCGGGGAA

ATGTGCGCGG

AACCCCTATT

TGTTTATTTT

121

TCTAAATACA

TTCAAATATG

TATCCGCTCA

TGAGACAATA

ACCCTGATAA

ATGCTTCAAT

181

AATATTGAAA

AAGGAAGAGT

ATGAGTATTC

AACATTTCCG

TGTCGCCCTT

ATTCCCTTTT

241

TTGCGGCATT

TTGCCTTCCT

GTTTTTGCTC

ACCCAGAAAC

GCTGGTGAAA

GTAAAAGATG

301

CTGAAGATCA

GTTGGGTGCA

CGAGTGGGTT

ACATCGAACT

GGATCTCAAC

AGCGGTAAGA

361

TCCTTGAGAG

TTTTCGCCCC

GAAGAACGTT

TTCCAATGAT

GAGCACTTTT

AAAGTTCTGC

421

TATGTGGCGC

GGTATTATCC

CGTATTGACG

CCGGGCAAGA

GCAACTCGGT

CGCCGCATAC

481

ACTATTCTCA

GAATGACTTG

GTTGAGTACT

CACCAGTCAC

AGAAAAGCAT

CTTACGGATG

541

GCATGACAGT

AAGAGAATTA

TGCAGTGCTG

CCATAACCAT

GAGTGATAAC

ACTGCGGCCA

601

ACTTACTTCT

GACAACGATC

GGAGGACCGA

AGGAGCTAAC

CGCTTTTTTG

CACAACATGG

661

GGGATCATGT

AACTCGCCTT

GATCGTTGGG

AACCGGAGCT

GAATGAAGCC

ATACCAAACG

721

ACGAGCGTGA

CACCACGATG

CCTGTAGCAA

TGGCAACAAC

GTTGCGCAAA

CTATTAACTG

781

GCGAACTACT

TACTCTAGCT

TCCCGGCAAC

AATTAATAGA

CTGGATGGAG

GCGGATAAAG

841

TTGCAGGACC

ACTTCTGCGC

TCGGCCCTTC

CGGCTGGCTG

GTTTATTGCT

GATAAATCTG

901

GAGCCGGTGA

GCGTGGGTCT

CGCGGTATCA

TTGCAGCACT

GGGGCCAGAT

GGTAAGCCCT

961

CCCGTATCGT

AGTTATCTAC

ACGACGGGGA

GTCAGGCAAC

TATGGATGAA

CGAAATAGAC

1021

AGATCGCTGA

GATAGGTGCC

TCACTGATTA

AGCATTGGTA

ACTGTCAGAC

CAAGTTTACT

1081

CATATATACT

TTAGATTGAT

TTAAAACTTC

ATTTTTAATT

TAAAAGGATC

TAGGTGAAGA

1141

TCCTTTTTGA

TAATCTCATG

ACCAAAATCC

CTTAACGTGA

GTTTTCGTTC

CACTGAGCGT

1201

CAGACCCCGT

AGAAAAGATC

AAAGGATCTT

CTTGAGATCC

TTTTTTTCTG

CGCGTAATCT

1261

GCTGCTTGCA

AACAAAAAAA

CCACCGCTAC

CAGCGGTGGT

TTGTTTGCCG

GATCAAGAGC

1321

TACCAACTCT

TTTTCCGAAG

GTAACTGGCT

TCAGCAGAGC

GCAGATACCA

AATACTGTTC

1381

TTCTAGTGTA

GCCGTAGTTA

GGCCACCACT

TCAAGAACTC

TGTAGCACCG

CCTACATACC

1441

TCGCTCTGCT

AATCCTGTTA

CCAGTGGCTG

CTGCCAGTGG

CGATAAGTCG

TGTCTTACCG

1501

GGTTGGACTC

AAGACGATAG

TTACCGGATA

AGGCGCAGCG

GTCGGGCTGA

ACGGGGGGTT

1561

CGTGCACACA

GCCCAGCTTG

GAGCGAACGA

CCTACACCGA

ACTGAGATAC

CTACAGCGTG

1621

AGCTATGAGA

AAGCGCCACG

CTTCCCGAAG

GGAGAAAGGC

GGACAGGTAT

CCGGTAAGCG

1681

GCAGGGTCGG

AACAGGAGAG

CGCACGAGGG

AGCTTCCAGG

GGGAAACGCC

TGGTATCTTT

1741

ATAGTCCTAT

CGGGTTTCGC

CACCTCTGAC

TTGAGCGTCG

ATTTTTGTGA

TGCTCGTCAG

1801

GGGGGCGGAG

CCTATGGAAA

AACGCCAGCA

ACGCGGCCTT

TTTACGGTTC

CTGGCCTTTT

1861

GCTGGCCTTT

TGCTCACATG

TTCTTTCCTG

CGTTATCCCC

TGATTCTGTG

GATAACCGTA

1921

TTACCGCCTT

TGAGTGAGCT

GATACCGCTC

GCCGCAGCCG

AACGACCGAG

CGCAGCGAGT

1981

CAGTGAGCGA

GGAAGCGGAA

GAGCGCCCAA

TACGCAAACC

GCCTCTCCCC

GCGCGTTGGC

2041

CGATTCATTA

ATGCAGCTGG

CACGACAGGT

TTCCCGACTG

GAAAGCGGGC

AGTGAGCGCA

2101

ACGCAATTAA

TGTGAGTTAG

CTCACTCATT

AGGCACCCCA

GGCTTTACAC

TTTATGCTTC

2161

CGGCTCGTAT

GTTGTGTGGA

ATTGTGAGCG

GATAACAATT

TCACACAGGA

AACAGCTATG

2221

ACCATGATTA

CGCCAAGCTC

CAACGATTTA

GGTGACACTA

TAGAAGAGAA

GGAATTAATA

2281

CGACTCACTA

TAGGGAGAGA

GAGAGAATTA

CCCTCACTAA

AGGGAGGAGA

AGCTTGCTTG

2341

TTCTTTTTGC

AGAAGCTCAG

AATAAACGCT

CAACTTTGGC

AGATCAAGCT

TGCATGCCTG

2401

CAGGTCGACT

ATAGGGAGAC

CCAAGCTTGG

CGCGCCGCCA

CCATGGACAA

GAAGTACTCC

2461

ATTGGGCTCG

CTATCGGCAC

AAACAGCGTC

GGCTGGGCCG

TCATTACGGA

CGAGTACAAG

2521

GTGCCGAGCA

AAAAATTCAA

AGTTCTGGGC

AATACCGATC

GCCACAGCAT

AAAGAAGAAC

2581

CTCATTGGCG

CCCTCCTGTT

CGACTCCGGG

GAGACGGCCG

AAGCCACGCG

GCTCAAAAGA

2641

ACAGCACGGC

GCAGATATAC

CCGCAGAAAG

AATCGGATCT

GCTACCTGCA

GGAGATCTTT

2701

AGTAATGAGA

TGGCTAAGGT

GGATGACTCT

TTCTTCCATA

GGCTGGAGGA

GTCCTTTTTG

2761

GTGGAGGAGG

ATAAAAAGCA

CGAGCGCCAC

CCAATCTTTG

GCAATATCGT

GGACGAGGTG

2821

GCGTACCATG

AAAAGTACCC

AACCATATAT

CATCTGAGGA

AGAAGCTTGT

AGACAGTACT

2881

GATAAGGCTG

ACTTGCGGTT

GATCTATCTC

GCGCTGGCGC

ATATGATCAA

ATTTCGGGGA

2941

CACTTCCTCA

TCGAGGGGGA

CCTGAACCCA

GACAACAGCG

ATGTCGACAA

ACTCTTTATC

3001

CAACTGGTTC

AGACTTACAA

TCAGCTTTTC

GAAGAGAACC

CGATCAACGC

ATCCGGAGTT

3061

GACGCCAAAG

CAATCCTGAG

CGCTAGGCTG

TCCAAATCCC

GGCGGCTCGA

AAACCTCATC

3121

GCACAGCTCC

CTGGGGAGAA

GAAGAACGGC

CTGTTTGGTA

ATCTTATCGC

CCTGTCACTC

3181

GGGCTGACCC

CCAACTTTAA

ATCTAACTTC

GACCTGGCCG

AAGATGCCAA

GCTTCAACTG

3241

AGCAAAGACA

CCTACGATGA

TGATCTCGAC

AATCTGCTGG

CCCAGATCGG

CGACCAGTAC

3301

GCAGACCTTT

TTTTGGCGGC

AAAGAACCTG

TCAGACGCCA

TTCTGCTGAG

TGATATTCTG

3361

CGAGTGAACA

CGGAGATCAC

CAAAGCTCCG

CTGAGCGCTA

GTATGATCAA

GCGCTATGAT

3421

GAGCACCACC

AAGACTTGAC

TTTGCTGAAG

GCCCTTGTCA

GACAGCAACT

GCCTGAGAAG

3481

TACAAGGAAA

TTTTCTTCGA

TCAGTCTAAA

AATGGCTACG

CCGGATACAT

TGACGGCGGA

3541

GCAAGCCAGG

AGGAATTTTA

CAAATTTATT

AAGCCCATCT

TGGAAAAAAT

GGACGGCACC

3601

GAGGAGCTGC

TGGTAAAGCT

TAACAGAGAA

GATCTGTTGC

GCAAACAGCG

CACTTTCGAC

3661

AATGGAAGCA

TCCCCCACCA

GATTCACCTG

GGCGAACTGC

ACGCTATCCT

CAGGCGGCAA

3721

GAGGATTTCT

ACCCCTTTTT

GAAAGATAAC

AGGGAAAAGA

TTGAGAAAAT

CCTCACATTT

3781

CGGATACCCT

ACTATGTAGG

CCCCCTCGCC

CGGGGAAATT

CCAGATTCGC

GTGGATGACT

3841

CGCAAATCAG

AAGAGACCAT

CACTCCCTGG

AACTTCGAGG

AAGTCGTGGA

TAAGGGGGCC

3901

TCTGCCCAGT

CCTTCATCGA

AAGGATGACT

AACTTTGATA

AAAATCTGCC

TAACGAAAAG

3961

GTGCTTCCTA

AACACTCTCT

GCTGTACGAG

TACTTCACAG

TTTATAACGA

GCTCACCAAG

4021

GTCAAATACG

TCACAGAAGG

GATGAGAAAG

CCAGCATTCC

TGTCTGGAGA

GCAGAAGAAA

4081

GCTATCGTGG

ACCTCCTCTT

CAAGACGAAC

CGGAAAGTTA

CCGTGAAACA

GCTCAAAGAA

4141

GACTATTTCA

AAAAGATTGA

ATGTTTCGAC

TCTGTTGAAA

TCAGCGGAGT

GGAGGATCGC

4201

TTCAACGCAT

CCCTGGGAAC

GTATCACGAT

CTCCTGAAAA

TCATTAAAGA

CAAGGACTTC

4261

CTGGACAATG

AGGAGAACGA

GGACATTCTT

GAGGACATTG

TCCTCACCCT

TACGTTGTTT

4321

GAAGATAGGG

AGATGATTGA

AGAACGCTTG

AAAACTTACG

CTCATCTCTT

CGACGACAAA

4381

GTCATGAAAC

AGCTCAAGAG

GCGCCGATAT

ACAGGATGGG

GGCGGCTGTC

AAGAAAACTG

4441

ATCAATGGGA

TCCGAGACAA

GCAGAGTGGA

AAGACAATCC

TGGATTTTCT

TAAGTCCGAT

4501

GGATTTGCCA

ACAGGAACTT

CATGCAGTTG

ATCCATGATG

ACTCTCTCAC

CTTTAAGGAG

4561

GACATCCAGA

AAGCACAAGT

TTCTGGCCAG

GGGGACAGTC

TTCACGAGCA

CATCGCTAAT

4621

CTTGCAGGTA

GCCCAGCTAT

CAAAAAGGGA

ATACTGCAGA

CCGTTAAGGT

CGTGGATGAA

4681

CTCGTCAAAG

TAATGGGAAG

GCATAAGCCC

GAGAATATCG

TTATCGAGAT

GGCCCGAGAG

4741

AACCAAACTA

CCCAGAAGGG

ACAGAAGAAC

AGTAGGGAAA

GGATGAAGAG

GATTGAAGAG

4801

GGTATAAAAG

AACTGGGGTC

CCAAATCCTT

AAGGAACACC

CAGTTGAAAA

CACCCAGCTT

4861

CAGAATGAGA

AGCTCTACCT

GTACTACCTG

CAGAACGGCA

GGGACATGTA

CGTGGATCAG

4921

GAACTGGACA

TCAATCGGCT

CTCCGACTAC

GACGTGGATG

CTATCGTGCC

CCAGTCTTTT

4981

CTCAAAGATG

ATTCTATTGA

TAATAAAGTG

TTGACAAGAT

CCGATAAAAA

TAGAGGGAAG

5041

AGTGATAACG

TCCCCTCAGA

AGAAGTTGTC

AAGAAAATGA

AAAATTATTG

GCGGCAGCTG

5101

CTGAACGCCA

AACTGATCAC

ACAACGGAAG

TTCGATAATC

TGACTAAGGC

TGAACGAGGT

5161

GGCCTGTCTG

AGTTGGATAA

AGCCGGCTTC

ATCAAAAGGC

AGCTTGTTGA

GACACGCCAG

5221

ATCACCAAGC

ACGTGGCCCA

AATTCTCGAT

TCACGCATGA

ACACCAAGTA

CGATGAAAAT

5281

GACAAACTGA

TTCGAGAGGT

GAAAGTTATT

ACTCTGAAGT

CTAAGCTGGT

CTCAGATTTC

5341

AGAAAGGACT

TTCAGTTTTA

TAAGGTGAGA

GAGATCAACA

ATTACCACCA

TGCGCATGAT

5401

GCCTACCTGA

ATGCAGTGGT

AGGCACTGCA

CTTATCAAAA

AATATCCCAA

GCTTGAATCT

5461

GAATTTGTTT

ACGGAGACTA

TAAAGTGTAC

GATGTTAGGA

AAATGATCGC

AAAGTCTGAG

5521

CAGGAAATAG

GCAAGGCCAC

CGCTAAGTAC

TTCTTTTACA

GCAATATTAT

GAATTTTTTC

5581

AAGACCGAGA

TTACACTGGC

CAATGGAGAG

ATTCGGAAGC

GACCACTTAT

CGAAACAAAC

5641

GGAGAAACAG

GAGAAATCGT

GTGGGACAAG

GGTAGGGATT

TCGCGACAGT

CCGGAAGGTC

5701

CTGTCCATGC

CGCAGGTGAA

CATCGTTAAA

AAGACCGAAG

TACAGACCGG

AGGCTTCTCC

5761

AAGGAAAGTA

TCCTCCCGAA

AAGGAACAGC

GACAAGCTGA

TCGCACGCAA

AAAAGATTGG

5821

GACCCCAAGA

AATACGGCGG

ATTCGATTCT

CCTACAGTCG

CTTACAGTGT

ACTGGTTGTG

5881

GCCAAAGTGG

AGAAAGGGAA

GTCTAAAAAA

CTCAAAAGCG

TCAAGGAACT

GCTGGGCATC

5941

ACAATCATGG

AGCGATCAAG

CTTCGAAAAA

AACCCCATCG

ACTTTCTCGA

GGCGAAAGGA

6001

TATAAAGAGG

TCAAAAAAGA

CCTCATCATT

AAGCTTCCCA

AGTACTCTCT

CTTTGAGCTT

6061

GAAAACGGCC

GGAAACGAAT

GCTCGCTAGT

GCGGGCGAGC

TGCAGAAAGG

TAACGAGCTG

6121

GCACTGCCCT

CTAAATACGT

TAATTTCTTG

TATCTGGCCA

GCCACTATGA

AAAGCTCAAA

6181

GGGTCTCCCG

AAGATAATGA

GCAGAAGCAG

CTGTTCGTGG

AACAACACAA

ACACTACCTT

6241

GATGAGATCA

TCGAGCAAAT

AAGCGAATTC

TCCAAAAGAG

TGATCCTCGC

CGACGCTAAC

6301

CTCGATAAGG

TGCTTTCTGC

TTACAATAAG

CACAGGGATA

AGCCCATCAG

GGAGCAGGCA

6361

GAAAACATTA

TCCACTTGTT

TACTCTGACC

AACTTGGGCG

CGCCTGCAGC

CTTCAAGTAC

6421

TTCGACACCA

CCATAGACAG

AAAGCGGTAC

ACCTCTACAA

AGGAGGTCCT

GGACGCCACA

6481

CTGATTCATC

AGTCAATTAC

GGGGCTCTAT

GAAACAAGAA

TCGACCTCTC

TCAGCTCGGT

6541

GGAGACGCTA

GCGGCTCTAG

AATGGTGAGC

AAGGGCGAGG

AGCTGTTCAC

CGGGGTGGTG

6601

CCCATCCTGG

TCGAGCTGGA

CGGCGACGTA

AACGGCCACA

AGTTCAGCGT

GTCCGGCGAG

6661

GGCGAGGGCG

ATGCCACCTA

CGGCAAGCTG

ACCCTGAAGT

TCATCTGCAC

CACCGGCAAG

6721

CTGCCCGTGC

CCTGGCCCAC

CCTCGTGACC

ACCCTGACCT

ACGGCGTGCA

GTGCTTCAGC

6781

CGCTACCCCG

ACCACATGAA

GCAGCACGAC

TTCTTCAAGT

CCGCCATGCC

CGAAGGCTAC

6841

GTCCAGGAGC

GCACCATCTT

CTTCAAGGAC

GACGGCAACT

ACAAGACCCG

CGCCGAGGTG

6901

AAGTTCGAGG

GCGACACCCT

GGTGAACCGC

ATCGAGCTGA

AGGGCATCGA

CTTCAAGGAG

6961

GACGGCAACA

TCCTGGGGCA

CAAGCTGGAG

TACAACTACA

ACAGCCACAA

CGTCTATATC

7021

ATGGCCGACA

AGCAGAAGAA

CGGCATCAAG

GTGAACTTCA

AGATCCGCCA

CAACATCGAG

7081

GACGGCAGCG

TGCAGCTCGC

CGACCACTAC

CAGCAGAACA

CCCCCATCGG

CGACGGCCCC

7141

GTGCTGCTGC

CCGACAACCA

CTACCTGAGC

ACCCAGTCCG

CCCTGAGCAA

AGACCCCAAC

7201

GAGAAGCGCG

ATCACATGGT

CCTGCTGGAG

TTCGTGACCG

CCGCCGGGAT

CACTCTCGGC

7261

ATGGACGAGC

TGTACAAGTA

GTGACTGACT

AGGATCTGGT

TACCACTAAA

CCAGCCTCAA

7321

GAACACCCGA

ATGGAGTCTC

TAAGCTACAT

AATACCAACT

TACACTTTAC

AAAATGTTGT

7381

CCCCCAAAAT

GTAGCCATTC

GTATCTGCTC

CTAATAAAAA

GAAAGTTTCT

TCACATTCTA

7441

AAAAAAAAAA

AAAAAAAAAA

AAAAAAAAAA

AnCCCCCCCC

CCCCAAGGGC

GAATTCACTG

7501

GCCGTCGTTT

TACAACGTCG

TGACTGGGAA

AACCCTGGCG

TTACCCAACT

TAATCGCCTT

7561

GCAGCACATC

CCCCTTTCGC

CAGCTGGCGT

AATAGCGAAG

AGGCCCGCAC

CGATCGCCCT

7621

TCCCAACAGT

TGCGCAGCCT

GAATGGCGAA

TGGCGCCTGA

TGCGGTATTT

TCTCCTTACG

7681

CATCTGTGCG

GTATTTCACA

CCGCATATGG

TGCACTCTCA

GTACAATCTG

CTCTGATGCC

7741

GCATAGTTAA

GCCAGCCCCG

ACACCCGCCA

ACACCCGCTG

ACGCGCCCTG

ACGGGCTTGT

7801

CTGCTCCCGG

CATCCGCTTA

CAGACAAGCT

GTGACCGTCT

CCGGGAGCTG

CATGTGTCAG

7861

AGGTTTTCAC

CGTCATCACC

GAAACGCGCG

A

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