名称: | EC2_10_dCas9_VPR_HC_sgRNA |
---|---|
别称: | Plasmid#163712 |
基因长: | |
质粒宿主: | 酵母菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | |
荧光标记: | |
启动子: | TEF1 |
复制子: | 2U,pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCCTGAATTC
GCATCTAGAC
TGAACTGGCC
GATAATTGCA
GACGAACGCC
TTGCCAACAG
GGAGTTCTTC
AGAGACATGG
AGGCTCAAAA
CGAAATTATT
GACAGCCTAG
ACATCAATAG
TCATACAACA
GAAAGCGACC
ACCCAACTTT
GGCTGATAAT
AGCGTATAAA
CAATGCATAC
TTTGTACGTT
CAAAATACAA
TGCAGTAGAT
ATATTTATGC
ATATTACATA
TAATACATAT
CACATAGGAA
GCAACAGGCG
CGTTGGACTT
TTAATTTTCG
AGGACCGCGA
ATCCTTACAT
CACACCCAAT
CCCCCACAAG
TGATCCCCCA
CACACCATAG
CTTCAAAATG
TTTCTACTCC
TTTTTTACTC
TTCCAGATTT
TCTCGGACTC
CGCGCATCGC
CGTACCACTT
CAAAACACCC
AAGCACAGCA
TACTAAATTT
CCCCTCTTTC
TTCCTCTAGG
GTGTCGTTAA
TTACCCGTAC
TAAAGGTTTG
GAAAAGAAAA
AAGACACCGC
CTCGTTTCTT
TTTCTTCGTC
GAAAAAGGCA
ATAAAAATTT
TTATCACGTT
TCTTTTTCTT
GAAAATTTTT
TTTTTTGATT
TTTTTCTCTT
TCGATGACCT
CCCATTGATA
TTTAAGTTAA
TAAACGGTCA
TCAATTTCTC
AAGTTTCAGT
TTCATTTTTC
TTGTTCTATT
ACAACTTTTT
TTACTTCTTG
CTCATTAGAA
AGAAAGCATA
GCAATCTAAT
CTAAGTTTTA
ATTACAAAAG
ATCTATGCCA
AAGAAGAAGA
GAAAGGTAGG
CAGCGACAAG
AAGTATTCTA
TCGGACTGGC
CATCGGGACT
AATAGCGTCG
GGTGGGCCGT
GATCACTGAC
GAGTACAAGG
TGCCCTCTAA
GAAGTTCAAG
GTGCTCGGGA
ACACCGACCG
GCATTCCATC
AAGAAAAATC
TGATCGGAGC
TCTCCTCTTT
GATTCAGGGG
AAACCGCTGA
AGCAACCCGC
CTCAAGCGGA
CTGCTAGACG
GCGGTACACC
AGGAGGAAGA
ACCGGATTTG
TTACCTTCAA
GAGATATTCT
CCAACGAAAT
GGCAAAGGTC
GACGACAGCT
TCTTCCATAG
GCTGGAAGAA
TCATTCCTCG
TGGAAGAGGA
TAAGAAGCAT
GAACGGCATC
CCATCTTCGG
TAATATCGTC
GACGAGGTGG
CCTATCACGA
GAAATACCCA
ACCATCTACC
ATCTTCGCAA
AAAGCTGGTG
GACTCAACCG
ACAAGGCAGA
CCTCCGGCTT
ATCTACCTGG
CCCTGGCCCA
CATGATCAAG
TTCAGAGGCC
ACTTCCTGAT
CGAGGGCGAC
CTCAATCCTG
ACAATAGCGA
TGTGGATAAA
CTGTTCATCC
AGCTGGTGCA
GACTTACAAC
CAGCTCTTTG
AAGAGAACCC
CATCAATGCA
AGCGGAGTCG
ATGCCAAGGC
CATTCTGTCA
GCCCGGCTGT
CAAAGAGCCG
CAGACTTGAG
AATCTTATCG
CTCAGCTGCC
GGGTGAAAAG
AAAAATGGAC
TGTTCGGGAA
CCTGATTGCT
CTTTCACTTG
GGCTGACTCC
CAATTTCAAG
TCTAATTTCG
ACCTGGCAGA
GGATGCCAAG
CTGCAACTGT
CCAAGGACAC
CTATGATGAC
GATCTCGACA
ACCTCCTGGC
CCAGATCGGT
GACCAATACG
CCGACCTTTT
CCTTGCTGCT
AAGAATCTTT
CTGACGCCAT
CCTGCTGTCT
GACATTCTCC
GCGTGAACAC
TGAAATCACC
AAGGCCCCTC
TTTCAGCTTC
AATGATTAAG
CGGTATGATG
AGCACCACCA
GGACCTGACC
CTGCTTAAGG
CACTCGTCCG
GCAGCAGCTT
CCGGAGAAGT
ACAAGGAAAT
CTTCTTTGAC
CAGTCAAAGA
ATGGATACGC
CGGCTACATC
GACGGAGGTG
CCTCCCAAGA
GGAATTTTAT
AAGTTTATCA
AACCTATCCT
TGAGAAGATG
GACGGCACCG
AAGAGCTCCT
CGTGAAACTG
AATCGGGAGG
ATCTGCTGCG
GAAGCAGCGC
ACTTTCGACA
ATGGGAGCAT
TCCCCACCAG
ATCCATCTTG
GGGAGCTTCA
CGCCATCCTT
CGGCGCCAAG
AGGACTTCTA
CCCCTTTCTT
AAGGACAACA
GGGAGAAGAT
TGAGAAAATT
CTCACTTTCC
GCATCCCCTA
CTACGTGGGA
CCCCTCGCCA
GAGGAAATAG
CCGGTTTGCT
TGGATGACCA
GAAAGTCAGA
AGAAACTATC
ACTCCCTGGA
ACTTCGAAGA
GGTGGTGGAC
AAGGGAGCCA
GCGCTCAGTC
ATTCATCGAA
CGGATGACTA
ACTTCGATAA
GAACCTCCCC
AATGAGAAGG
TCCTGCCGAA
ACATTCCCTG
CTCTACGAGT
ACTTTACCGT
GTACAACGAG
CTGACCAAGG
TGAAATATGT
CACCGAAGGG
ATGAGGAAGC
CCGCATTCCT
GTCAGGCGAA
CAAAAGAAGG
CAATTGTGGA
CCTTCTGTTC
AAGACCAATA
GAAAGGTGAC
CGTGAAGCAG
CTGAAGGAGG
ACTATTTCAA
GAAAATTGAA
TGCTTCGACT
CTGTGGAGAT
TAGCGGGGTC
GAAGATCGGT
TCAACGCAAG
CCTGGGTACC
TACCATGATC
TGCTTAAGAT
CATCAAGGAC
AAGGATTTTC
TGGACAATGA
GGAGAAAGAG
GACATCCTTG
AGGACATTGT
CCTGACTCTC
ACTCTGTTCG
AGGACCGGGA
AATGATCGAG
GAGAGGCTTA
AGACCTACGC
CCATCTGTTC
GACGATAAAG
TGATGAAGCA
ACTTAAACGG
AGAAGATATA
CCGGATGGGG
ACGCCTTAGC
CGCAAACTCA
TCAACGGAAT
CCGGGACAAA
CAGAGCGGAA
AGACCATTCT
TGATTTCCTT
AAGAGCGACG
GATTCGCTAA
TCGCAACTTC
ATGCAACTTA
TCCATGATGA
TTCCCTGACC
TTTAAGGAGG
ACATCCAGAA
GGCCCAAGTG
TCTGGACAAG
GTGACTCACT
GCACGAGCAT
ATCGCAAATC
TGGCTGGTTC
ACCCGCTATT
AAGAAGGGTA
TTCTCCAGAC
CGTGAAAGTC
GTGGACGAGC
TGGTCAAGGT
GATGGGTCGC
CATAAACCAG
AGAACATTGT
CATCGAGATG
GCCAGGGAAA
ACCAGACTAC
CCAGAAGGGA
CAGAAGAACA
GCAGGGAGCG
GATGAAAAGA
ATTGAGGAAG
GGATTAAGGA
GCTCGGGTCA
CAGATCCTTA
AAGAGCACCC
GGTGGAAAAC
ACCCAGCTTC
AGAATGAGAA
GCTCTATCTG
TACTACCTTC
AAAATGGACG
CGATATGTAT
GTGGACCAAG
AGCTTGATAT
CAACAGGCTC
TCAGACTACG
ACGTGGACGC
CATCGTCCCT
CAGAGCTTCC
TCAAAGACGA
CTCAATTGAC
AATAAGGTGC
TGACTCGCTC
AGACAAGAAC
CGGGGAAAGT
CAGATAACGT
GCCCTCAGAG
GAAGTCGTGA
AAAAGATGAA
GAACTATTGG
CGCCAGCTTC
TGAACGCAAA
GCTGATCACT
CAGCGGAAGT
TCGACAATCT
CACTAAGGCT
GAGAGGGGCG
GACTGAGCGA
ACTGGACAAA
GCAGGATTCA
TTAAACGGCA
ACTTGTGGAG
ACTCGGCAGA
TTACTAAACA
TGTAGCCCAA
ATCCTTGACT
CACGCATGAA
TACCAAGTAC
GACGAAAACG
ACAAACTTAT
CCGCGAGGTG
AAGGTGATTA
CCCTGAAGTC
CAAGCTGGTC
AGCGATTTCA
GAAAGGACTT
TCAATTCTAC
AAAGTGCGGG
AGATCAATAA
CTATCATCAT
GCTCATGACG
CATATCTGAA
TGCCGTGGTG
GGAACCGCCC
TGATCAAGAA
GTACCCAAAG
CTGGAAAGCG
AGTTCGTGTA
CGGAGACTAC
AAGGTCTACG
ACGTGCGCAA
GATGATTGCC
AAATCTGAGC
AGGAGATCGG
AAAGGCCACC
GCAAAGTACT
TCTTCTACAG
CAACATCATG
AATTTCTTCA
AGACCGAAAT
CACCCTTGCA
AACGGTGAGA
TCCGGAAGAG
GCCGCTCATC
GAGACTAATG
GGGAGACTGG
CGAAATCGTG
TGGGACAAGG
GCAGAGATTT
CGCTACCGTG
CGCAAAGTGC
TTTCTATGCC
TCAAGTGAAC
ATCGTGAAGA
AAACCGAGGT
GCAAACCGGA
GGCTTTTCTA
AGGAATCAAT
CCTCCCCAAG
CGCAACTCCG
ACAAGCTCAT
TGCAAGGAAG
AAGGATTGGG
ACCCTAAGAA
GTACGGCGGA
TTCGATTCAC
CAACTGTGGC
TTATTCTGTC
CTGGTCGTGG
CTAAGGTGGA
AAAAGGAAAG
TCTAAGAAGC
TCAAGAGCGT
GAAGGAACTG
CTGGGTATCA
CCATTATGGA
GCGCAGCTCC
TTCGAGAAGA
ACCCAATTGA
CTTTCTCGAA
GCCAAAGGTT
ACAAGGAAGT
CAAGAAGGAC
CTTATCATCA
AGCTCCCAAA
GTATAGCCTG
TTCGAACTGG
AGAATGGGCG
GAAGCGGATG
CTCGCCTCCG
CTGGCGAACT
TCAGAAGGGT
AATGAGCTGG
CTCTCCCCTC
CAAGTACGTG
AATTTCCTCT
ACCTTGCAAG
CCATTACGAG
AAGCTGAAGG
GGAGCCCCGA
GGACAACGAG
CAAAAGCAAC
TGTTTGTGGA
GCAGCATAAG
CATTATCTGG
ACGAGATCAT
TGAGCAGATT
TCCGAGTTTT
CTAAACGCGT
CATTCTCGCT
GATGCCAACC
TCGATAAAGT
CCTTAGCGCA
TACAATAAGC
ACAGAGACAA
ACCAATTCGG
GAGCAGGCTG
AGAATATCAT
CCACCTGTTC
ACCCTCACCA
ATCTTGGTGC
CCCTGCCGCA
TTCAAGTACT
TCGACACCAC
CATCGACCGG
AAACGCTATA
CCTCCACCAA
AGAAGTGCTG
GACGCCACCC
TCATCCACCA
GAGCATCACC
GGACTTTACG
AAACTCGGAT
TGACCTCTCA
CAGCTCGGAG
GGGATCCCAA
GAAAAAGCGC
AAGGTAGGAT
CCGACGCATT
GGACGATTTT
GATCTGGATA
TGCTGGGAAG
TGACGCCCTC
GATGATTTTG
ACCTTGACAT
GCTTGGTTCG
GATGCCCTTG
ATGACTTTGA
CCTCGACATG
CTCGGCAGTG
ACGCCCTTGA
TGATTTCGAC
CTGGACATGC
TGATTAACTC
TAGAAGTTCC
GGATCTCCGA
AAAAGAAACG
CAAAGTTGGT
AGCCAGTACC
TGCCCGACAC
CGACGACCGG
CACCGGATCG
AGGAAAAGCG
GAAGCGGACC
TACGAGACAT
TCAAGAGCAT
CATGAAGAAG
TCCCCCTTCA
GCGGCCCCAC
CGACCCTAGA
CCTCCACCTA
GAAGAATCGC
CGTGCCCAGC
AGATCCAGCG
CCAGCGTGCC
AAAACCTGCC
CCCCAGCCTT
ACCCCTTCAC
CAGCAGCCTG
AGCACCATCA
ACTACGACGA
GTTCCCTACC
ATGGTGTTCC
CCAGCGGCCA
GATCTCTCAG
GCCTCTGCTC
TGGCTCCAGC
CCCTCCTCAG
GTGCTGCCTC
AGGCTCCTGC
TCCTGCACCA
GCTCCAGCCA
TGGTGTCTGC
ACTGGCTCAG
GCACCAGCAC
CCGTGCCTGT
GCTGGCTCCT
GGACCTCCAC
AGGCTGTGGC
TCCACCAGCC
CCTAAACCTA
CACAGGCCGG
CGAGGGCACA
CTGTCTGAAG
CTCTGCTGCA
GCTGCAGTTC
GACGACGAGG
ATCTGGGAGC
CCTGCTGGGA
AACAGCACCG
ATCCTGCCGT
GTTCACCGAC
CTGGCCAGCG
TGGACAACAG
CGAGTTCCAG
CAGCTGCTGA
ACCAGGGCAT
CCCTGTGGCC
CCTCACACCA
CCGAGCCCAT
GCTGATGGAA
TACCCCGAGG
CCATCACCCG
GCTCGTGACA
GGCGCTCAGA
GGCCTCCTGA
TCCAGCTCCT
GCCCCTCTGG
GAGCACCAGG
CCTGCCTAAT
GGACTGCTGT
CTGGCGACGA
GGACTTCAGC
TCTATCGCCG
ACATGGACTT
CTCCGCACTG
CTGGGTAGCG
GATCGGGATC
TCGGGATTCC
AGGGAAGGGA
TGTTTTTGCC
GAAGCCTGAG
GCCGGCTCCG
CTATTAGTGA
CGTGTTTGAG
GGCCGCGAGG
TGTGCCAGCC
AAAACGAATC
CGGCCATTTC
ATCCTCCAGG
AAGTCCATGG
GCCAACCGCC
CACTCCCCGC
CAGCCTCGCA
CCAACACCAA
CCGGTCCAGT
ACATGAGCCA
GTCGGGTCAC
TGACCCCGGC
ACCAGTCCCT
CAGCCACTGG
ATCCAGCGCC
CGCAGTGACT
CCCGAGGCCA
GTCACCTGTT
GGAGGATCCC
GATGAAGAAA
CGAGCCAGGC
TGTCAAAGCC
CTTCGGGAGA
TGGCCGATAC
TGTGATTCCC
CAGAAGGAAG
AGGCTGCAAT
CTGTGGCCAA
ATGGACCTTT
CCCATCCGCC
CCCAAGGGGC
CATCTGGATG
AGCTGACAAC
CACACTTGAG
TCCATGACCG
AGGATCTGAA
CCTGGACTCA
CCCCTGACCC
CGGAATTGAA
CGAGATTCTG
GATACCTTCC
TGAACGACGA
GTGCCTCTTG
CATGCCATGC
ATATCAGCAC
AGGACTGTCC
ATCTTCGACA
CATCTCTGTT
TTAACTCGAG
TGGCATAAAG
CAATCTTGAT
GAGGATAATG
ATTTTTTTTT
GAATATACAT
AAATACTACC
GTTTTTCTGC
TAGATTTTGT
GATGACGTAA
ATAAGTACAT
ATTACTTTTT
AAGCCAAGAC
AAGATTAAGC
ATTAACTTTA
CCCTTTTCTT
TCTAAGTTTC
AATATTAGTT
ATCACTGTTT
AAAAGTTATG
GCGAGAACGT
CGGCGGTTAA
AATATATTAC
CCTGAACGGC
TGCCAAACCA
GATGTCAACA
CAGCTACAAC
GATGTGCTTC
AGTATTACAT
TTTTTGCCTT
CAACGCCTTG
ATTGTTCTAT
TTTTGCTAAT
AATAAATCTA
TTTCATCGGA
CTAAAAGTCC
ATTAGTTGTA
AGCGGATTTA
GCTCAGTTGG
GAGAGCGCCA
GACTGAAGAA
AAACTTCGGT
CAAGTCATCT
GGAGGTCCTG
TGTTCGATCC
ACAGAATTCG
CAGATGGCCG
GCATGGTCCC
AGCCTCCTCG
CTGGCGCCGG
CTGGGCAACA
CCTTCGGGTG
GCGAATGGGA
CTCGCTATCG
TCTTCTCACG
AAGACTTACT
CAGTTTTAGA
GCTAGAAATA
GCAAGTTAAA
ATAAGGCTAG
TCCGTTATCA
ACTTGAAAAA
GTGGCACCGA
GTCGGTGCTT
TTTTTATTTT
TTGTCACTAT
TGTTATGTAA
AATGCCACCT
CTGACAGTAT
GGAACGCAAA
CTTCTGTCTA
GTGGATAGCT
GAGCAGTTAC
AGAGATGTTA
CGAACCACTA
GTGCACTGCA
GTACAGTTTA
GCTTGCCTCG
TCCCCGCCGG
GTCACCCGGC
CAGCGACATG
GAGGCCCAGA
ATACCCTCCT
TGACAGTCTT
GACGTGCGCA
GCTCAGGGGC
ATGATGTGAC
TGTCGCCCGT
ACATTTAGCC
CATACATCCC
CATGTATAAT
CATTTGCATC
CATACATTTT
GATGGCCGCA
CGGCGCGAAG
CAAAAATTAC
GGCTCCTCGC
TCCAGACCTG
CGAGCAGGGA
AACGCTCCCC
TCACAGACGC
GTTGAATTGT
CCCCACGCCG
CGCCCCTGTA
GAGAAATATA
AAAGGTTAGG
ATTTGCCACT
GAGGTTCTTC
TTTCATATAC
TTCCTTTTAA
AATCTTGCTA
GGATACAGTT
CTCACATCAC
ATCCGAACAT
AAACAAAAAT
GGGTAAGGAA
AAGACTCACG
TTTCGAGGCC
GCGATTAAAT
TCCAACATGG
ATGCTGATTT
ATATGGGTAT
AAATGGGCTC
GCGATAATGT
CGGGCAATCA
GGTGCGACAA
TCTATCGATT
GTATGGGAAG
CCCGATGCGC
CAGAGTTGTT
TCTGAAACAT
GGCAAAGGTA
GCGTTGCCAA
TGATGTTACA
GATGAGATGG
TCAGACTAAA
CTGGCTGACG
GAATTTATGC
CTCTTCCGAC
CATCAAGCAT
TTTATCCGTA
CTCCTGATGA
TGCATGGTTA
CTCACCACTG
CGATCCCCGG
CAAAACAGCA
TTCCAGGTAT
TAGAAGAATA
TCCTGATTCA
GGTGAAAATA
TTGTTGATGC
GCTGGCAGTG
TTCCTGCGCC
GGTTGCATTC
GATTCCTGTT
TGTAATTGTC
CTTTTAACAG
CGATCGCGTA
TTTCGTCTGG
CTCAGGCGCA
ATCACGAATG
AATAACGGTT
TGGTTGATGC
GAGTGATTTT
GATGACGAGC
GTAATGGCTG
GCCTGTTGAA
CAAGTCTGGA
AAGAAATGCA
TAAGCTTTTG
CCATTCTCAC
CGGATTCAGT
CGTCACTCAT
GGTGATTTCT
CACTTGATAA
CCTTATTTTT
GACGAGGGGA
AATTAATAGG
TTGTATTGAT
GTTGGACGAG
TCGGAATCGC
AGACCGATAC
CAGGATCTTG
CCATCCTATG
GAACTGCCTC
GGTGAGTTTT
CTCCTTCATT
ACAGAAACGG
CTTTTTCAAA
AATATGGTAT
TGATAATCCT
GATATGAATA
AATTGCAGTT
TCATTTGATG
CTCGATGAGT
TTTTCTAAAG
TAACTGACAA
TAAAAAGATT
CTTGTTTTCA
AGAACTTGTC
ATTTGTATAG
TTTTTTTATA
TTGTAGTTGT
TCTATTTTAA
TCAAATGTTA
GCGTGATTTA
TATTTTTTTT
CGCCTCGACA
TCATCTGCCC
AGATGCGAAG
TTAAGTGCGC
AGAAAGTAAT
ATCATGCGTC
AATCGTATGT
GAATGCTGGT
CGCTATACTG
GAGTAACGAA
GCATCTGTGC
TTCATTTTGT
AGAACAAAAA
TGCAACGCGA
GAGCGCTAAT
TTTTCAAACA
AAGAATCTGA
GCTGCATTTT
TACAGAACAG
AAATGCAACG
CGAAAGCGCT
ATTTTACCAA
CGAAGAATCT
GTGCTTCATT
TTTGTAAAAC
AAAAATGCAA
CGCGAGAGCG
CTAATTTTTC
AAACAAAGAA
TCTGAGCTGC
ATTTTTACAG
AACAGAAATG
CAACGCGAGA
GCGCTATTTT
ACCAACAAAG
AATCTATACT
TCTTTTTTGT
TCTACAAAAA
TGCATCCCGA
GAGCGCTATT
TTTCTAACAA
AGCATCTTAG
ATTACTTTTT
TTCTCCTTTG
TGCGCTCTAT
AATGCAGTCT
CTTGATAACT
TTTTGCACTG
TAGGTCCGTT
AAGGTTAGAA
GAAGGCTACT
TTGGTGTCTA
TTTTCTCTTC
CATAAAAAAA
GCCTGACTCC
ACTTCCCGCG
TTTACTGATT
ACTAGCGAAG
CTGCGGGTGC
ATTTTTTCAA
GATAAAGGCA
TCCCCGATTA
TATTCTATAC
CGATGTGGAT
TGCGCATACT
TTGTGAACAG
AAAGTGATAG
CGTTGATGAT
TCTTCATTGG
TCAGAAAATT
ATGAACGGTT
TCTTCTATTT
TGTCTCTATA
TACTACGTAT
AGGAAATGTT
TACATTTTCG
TATTGTTTTC
GATTCACTCT
ATGAATAGTT
CTTACTACAA
TTTTTTTGTC
TAAAGAGTAA
TACTAGAGAT
AAACATAAAA
AATGTAGAGG
TCGAGTTTAG
ATGCAAGTTC
AAGGAGCGAA
AGGTGGATGG
GTAGGTTATA
TAGGGATATA
GCACAGAGAT
ATATAGCAAA
GAGATACTTT
TGAGCAATGT
TTGTGGAAGC
GGTATTCGCA
ATATTTTAGT
AGCTCGTTAC
AGTCCGGTGC
GTTTTTGGTT
TTTTGAAAGT
GCGTCATCAG
AGCGCTTTTG
GTTTTCAAAA
GCGCTCTGAA
GTTCCTATAC
TTTCTAGCTA
GAGAATAGGA
ACTTCCCGAG
CGGCCGCGTG
TTACAACCAA
TTAACCAATT
CTGATTAGAA
AAACTCATCG
AGCATCAAAT
GAAACTGCAA
TTTATTCATA
TCAGGATTAT
CAATACCATA
TTTTTGAAAA
AGCCGTTTCT
GTAATGAAGG
AGAAAACTCA
CCGAGGCAGT
TCCATAGGAT
GGCAAGATCC
TGGTATCGGT
CTGCGATTCC
GACTCGTCCA
ACATCAATAC
AACCTATTAA
TTTCCCCTCG
TCAAAAATAA
GGTTATCAAG
TGAGAAATCA
CCATGAGTGA
CGACTGAATC
CGGTGAGAAT
GGCAAAAGCT
TATGCATTTC
TTTCCAGACT
TGTTCAACAG
GCCAGCCATT
ACGCTCGTCA
TCAAAATCAC
TCGCATCAAC
CAAACCGTTA
TTCATTCGTG
ATTGCGCCTG
AGCGAGGCGA
AATACGCGAT
CGCTGTTAAA
AGGACAATTA
CAAACAGGAA
TCGAATGCAA
CCGGCGCAGG
AACACTGCCA
GCGCATCAAC
AATATTTTCA
CCTGAATCAG
GATATTCTTC
TAATACCTGG
AATGCTGTTT
TCCCGGGGAT
CGCAGTGGTG
AGTAACCATG
CATCATCAGG
AGTACGGATA
AAATGCTTGA
TGGTCGGAAG
AGGCATAAAT
TCCGTCAGCC
AGTTTAGTCT
GACCATCTCA
TCTGTAACAT
CATTGGCAAC
GCTACCTTTG
CCATGTTTCA
GAAACAACTC
TGGCGCATCG
GGCTTCCCAT
ACAATCGATA
GATTGTCGCA
CCTGATTGCC
CGACATTATC
GCGAGCCCAT
TTATACCCAT
ATAAATCAGC
ATCCATGTTG
GAATTTAATC
GCGGCCTGGA
GCAAGACGTT
TCCCGTTGAA
TATGGCTCAT
AACACCCCTT
GTATTACTGT
TTATGTAAGC
AGACAGTTTT
ATTGTTCATG
ATGATATATT
TTTATCTTGT
GCAATGTAAC
ATCAGAGATT
TTGAGACACA
ACGTGGCTTT
GTTGAATAAA
TCGAACTTTT
GCTGAGTTGA
AGGATCAGTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TTCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGTTCTTTC
CTGCGTTATC
CCCTGATTCT
GTGGATAACC
GTGCGGCCGC