名称: | pDRf1-GW |
---|---|
别称: | Plasmid#36026 |
基因长: | |
质粒宿主: | 酵母菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF,GateWay |
片段物种: | |
原核抗性: | Amp |
筛选标记: | URA3 |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DB3.1 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACTAGTGAAT
TCGCGGCCAT
CACAAGTTTG
TACAAAAAAG
CTGAACGAGA
AACGTAAAAT
GATATAAATA
TCAATATATT
AAATTAGATT
TTGCATAAAA
AACAGACTAC
ATAATACTGT
AAAACACAAC
ATATCCAGTC
ACTATGGCGG
CCCCTCGAGG
GGCCGCATTA
GGCACCCCAG
GCTTTACACT
TTATGCTTCC
GGCTCGTATA
ATGTGTGGAT
TTTGAGTTAG
GATCCGTCGA
GATTTTCAGG
AGCTAAGGAA
GCTAAAATGG
AGAAAAAAAT
CACTGGATAT
ACCACCGTTG
ATATATCCCA
ATGGCATCGT
AAAGAACATT
TTGAGGCATT
TCAGTCAGTT
GCTCAATGTA
CCTATAACCA
GACCGTTCAG
CTGGATATTA
CGGCCTTTTT
AAAGACCGTA
AAGAAAAATA
AGCACAAGTT
TTATCCGGCC
TTTATTCACA
TTCTTGCCCG
CCTGATGAAT
GCTCATCCGG
AATTCCGTAT
GGCAATGAAA
GACGGTGAGC
TGGTGATATG
GGATAGTGTT
CACCCTTGTT
ACACCGTTTT
CCATGAGCAA
ACTGAAACGT
TTTCATCGCT
CTGGAGTGAA
TACCACGACG
ATTTCCGGCA
GTTTCTACAC
ATATATTCGC
AAGATGTGGC
GTGTTACGGT
GAAAACCTGG
CCTATTTCCC
TAAAGGGTTT
ATTGAGAATA
TGTTTTTCGT
CTCAGCCAAT
CCCTGGGTGA
GTTTCACCAG
TTTTTGATTT
AAACGTGGCC
AATATGGACA
ACTTCTTCGC
CCCCGTTTTC
ACCATGGGCA
AATATTATAC
GCAAGGGCGA
CAAGGTGCTG
ATGCCGCTGG
CGATTCAGGT
TCATCATGCC
GTTTGTGATG
GCTTCCATGT
CGGGCAGAAT
GCTTAATGAA
TTACAACAGT
ACTGCGATGA
GTGGCAGGGC
GGGGCGTAAn
CGCGTGGATC
CGGCTTACTA
AAAGCCAGAT
AACAGTATGC
GTATTTGCGC
GCTGATTTTT
GCGGTATAAG
AATATATACT
GATATGTATA
CCCGAAGTAT
GTCAAAAAGA
GGTATGCTAT
GAAGCAGCGT
ATTACAGnGA
CAGTTGACAG
CGACAGCTAT
CAGTTGCTCA
AGGCATATAT
GATGTCAATA
TCTCCGGTCT
GGTAAGCACA
ACCATGCAGA
ATGAAGCCCG
TCGTCTGCGT
GCCGAACGCT
GGAAAGCGGA
AAATCAGGAA
GGGATGGCTG
AGGTCGCCCG
GTTTATTGAA
ATGAACGGCT
CTTTTGCTGA
CGAGAACAGG
GGCTGGTGAA
ATGCAGTTTA
AGGTTTACAC
CTATAAAAGA
GAGAGCCGTT
ATCGTCTGTT
TGTGGATGTA
CAGAGTGATA
TTATTGACAC
GCCCGGGCGA
CGGATGGTGA
TCCCCCTGGC
CAGTGCACGT
CTGCTGTCAG
ATAAAGTCnC
CCGTGAACTT
TACCCGGTGG
TGCATATCGG
GGATGAAAGC
TGGCGCATGA
TGACCACCGA
TATGGCCAGT
GTGCCGGTCT
CCGTTATCGG
GGAAGAAGTG
GCTGATCTCA
GCCACCGCGA
AAATGACATC
AAAAACGCCA
TTAACCTGAT
GTTCTGGGGA
ATATAAATGT
CAGGCTCCCT
TATACACAGC
CAGTCTGCAG
GTCGACCATA
GTGACTGGAT
ATGTTGTGTT
TTACAGTATT
ATGTAGTCTG
TTTTTTATGC
AAAATCTAAT
TTAATATATT
GATATTTATA
TCATTTTACG
TTTCTCGTTC
AGCTTTCTTG
TACAAAGTGG
TGTAGTTAAT
TCATGATGGC
CGCTGCAGGT
CGACCTCGAG
GGGGGGCCCG
GTACCCAATT
CGCCCTATAG
TGAGTCGTAT
TACGCGCGGA
TCCAGCTTTG
GACTTCTTCG
CCAGAGGTTT
GGTCAAGTCT
CCAATCAAGG
TTGTCGGCTT
GTCTACCTTG
CCAGAAATTT
ACGAAAAGAT
GGAAAAGGGT
CAAATCGTTG
GTAGATACGT
TGTTGACACT
TCTAAATAAG
CGAATTTCTT
ATGATTTATG
ATTTTTATTA
TTAAATAAGT
TATAAAAAAA
ATAAGTGTAT
ACAAATTTTA
AAGTGACTCT
TAGGTTTTAA
AACGAnAATT
CTTATTCTTG
AGTAACTCTT
TCCTGTAGGT
CAGGTTGCTT
TCTCAGGTAT
AGCATGAGGT
CGCTCTTATT
GACCACACCT
CTACCGGCAT
GCCAATTCAC
TGGCCGTCGT
TTTACAACGT
CGTGACTGGG
AAAACCCTGG
CGTTACCCAA
CTTAATCGCC
TTGCAGCACA
TCCCCCTTTC
GCCAGCTGGC
GTAATAGCGA
AGAGGCCCGC
ACCGATCGCC
CTTCCCAACA
GTTGCGCAGC
CTGAATGGCG
AATGGCGCCT
GATGCGGTAT
TTTCTCCTTA
CGCATCTGTG
CGGTATTTCA
CACCGCATAA
TCGGATCGTA
CTTGTTACCC
ATCATTGAAT
TTTGAACATC
CGAACCTGGG
AGTTTTCCCT
GAAACAGATA
GTATATTTGA
ACCTGTATAA
TAATATATAG
TCTAGCGCTT
TACGGAAGAC
AATGTATGTA
TTTCGGTTCC
TGGAGAAACT
ATTGCATCTA
TTGCATAGGT
AATCTTGCAC
GTCGCATCCC
CGGTTCATTT
TCTGCGTTTC
CATCTTGCAC
TTCAATAGCA
TATCTTTGTT
AACGAAGCAT
CTGTGCTTCA
TTTTGTAGAA
CAAAAATGCA
ACGCGAGAGC
GCTAATTTTT
CAAACAAAGA
ATCTGAGCTG
CATTTTTACA
GAACAGAAAT
GCAACGCGAA
AGCGCTATTT
TACCAACGAA
GAATCTGTGC
TTCATTTTTG
TAAAACAAAA
ATGCAACGCG
AGAGCGCTAA
TTTTTCAAAC
AAAGAATCTG
AGCTGCATTT
TTACAGAACA
GAAATGCAAC
GCGAGAGCGC
TATTTTACCA
ACAAAGAATC
TATACTTCTT
TTTTGTTCTA
CAAAAATGCA
TCCCGAGAGC
GCTATTTTTC
TAACAAAGCA
TCTTAGATTA
CTTTTTTTCT
CCTTTGTGCG
CTCTATAATG
CAGTCTCTTG
ATAACTTTTT
GCACTGTAGG
TCCGTTAAGG
TTAGAAGAAG
GCTACTTTGG
TGTCTATTTT
CTCTTCCATA
AAAAAAGCCT
GACTCCACTT
CCCGCGTTTA
CTGATTACTA
GCGAAGCTGC
GGGTGCATTT
TTTCAAGATA
AAGGCATCCC
CGATTATATT
CTATACCGAT
GTGGATTGCG
CATACTTTGT
GAACAGAAAG
TGATAGCGTT
GATGATTCTT
CATTGGTCAG
AAAATTATGA
ACGGTTTCTT
CTATTTTGTC
TCTATATACT
ACGTATAGGA
AATGTTTACA
TTTTCGTATT
GTTTTCGATT
CACTCTATGA
ATAGTTCTTA
CTACAATTTT
TTTGTCTAAA
GAGTAATACT
AGAGATAAAC
ATAAAAAATG
TAGAGGTCGA
GTTTAGATGC
AAGTTCAAGG
AGCGAAAGGT
GGATGGGTAG
GTTATATAGG
GATATAGCAC
AGAGATATAT
AGCAAAGAGA
TACTTTTGAG
CAATGTTTGT
GGAAGCGGTA
TTCGCAATAT
TTTAGTAGCT
CGTTACAGTC
CGGTGCGTTT
TTGGTTTTTT
GAAAGTGCGT
CTTCAGAGCG
CTTTTGGTTT
TCAAAAGCGC
TCTGAAGTTC
CTATACTTTC
TAGCTAGAGA
ATAGGAACTT
CGGAATAGGA
ACTTCAAAGC
GTTTCCGAAA
ACGAGCGCTT
CCGAAAATGC
AACGCGAGCT
GCGCACATAC
AGCTCACTGT
TCACGTCGCA
CCTATATCTG
CGTGTTGCCT
GTATATATAT
ATACATGAGA
AGAACGGCAT
AGTGCGTGTT
TATGCTTAAA
TGCGTACTTA
TATGCGTCTA
TTTATGTAGG
ATGAAAGGTA
GTCTAGTACC
TCCTGTGATA
TTATCCCATT
CCATGCGGGG
TATCGTATGC
TTCCTTCAGC
ACTACCCTTT
AGCTGTTCTA
TATGCTGCCA
CTCCTCAATT
GGATTAGTCT
CATCCTTCAA
TGCTATCATT
TCCTTTGATA
TTGGATCGAT
CCGATGATAA
GCTGTCAAAC
ATGAGAATTG
GGTAATAACT
GATATAATTA
AATTGAAGCT
CTAATTTGTG
AGTTTAGTAT
ACATGCATTT
ACTTATAATA
CAGTTTTTTA
GTTTTGCTGG
CCGCATCTTC
TCAAATATGC
TTCCCAGCCT
GCTTTTCTGT
AACGTTCACC
CTCTACCTTA
GCATCCCTTC
CCTTTGCAAA
TAGTCCTCTT
CCAACAATAA
TAATGTCAGA
TCCTGTAGAG
ACCACATCAT
CCACGGTTCT
ATACTGTTGA
CCCAATGCGT
CTCCCTTGTC
ATCTAAACCC
ACACCGGGTG
TCATAATCAA
CCAATCGTAA
CCTTCATCTC
TTCCACCCAT
GTCTCTTTGA
GCAATAAAGC
CGATAACAAA
ATCTTTGTCG
CTCTTCGCAA
TGTCAACAGT
ACCCTTAGTA
TATTCTCCAG
TAGATAGGGA
GCCCTTGCAT
GACAATTCTG
CTAACATCAA
AAGGCCTCTA
GGTTCCTTTG
TTACTTCTTC
TGCCGCCTGC
TTCAAACCGC
TAACAATACC
TGGGCCCACC
ACACCGTGTG
CATTCGTAAT
GTCTGCCCAT
TCTGCTATTC
TGTATACACC
CGCAGAGTAC
TGCAATTTGA
CTGTATTACC
AATGTCAGCA
AATTTTCTGT
CTTCGAAGAG
TAAAAAATTG
TACTTGGCGG
ATAATGCCTT
TAGCGGCTTA
ACTGTGCCCT
CCATGGAAAA
ATCAGTCAAG
ATATCCACAT
GTGTTTTTAG
TAAACAAATT
TTGGGACCTA
ATGCTTCAAC
TAACTCCAGT
AATTCCTTGG
TGGTACGAAC
ATCCAATGAA
GCACACAAGT
TTGTTTGCTT
TTCGTGCATG
ATATTAAATA
GCTTGGCAGC
AACAGGACTA
GGATGAGTAG
CAGCACGTTC
CTTATATGTA
GCTTTCGACA
TGATTTATCT
TCGTTTCCTG
CATGTTTTTG
TTCTGTGCAG
TTGGGTTAAG
AATACTGGGC
AATTTCATGT
TTCTTCAACA
CTACATATGC
GTATATATAC
CAATCTAAGT
CTGTGCTCCT
TCCTTCGTTC
TTCCTTCTGT
TCGGAGATTA
CCGAATCAAA
AAAATTTCAA
GGAAACCGAA
ATCAAAAAAA
AGAATAAAAA
AAAAATGATG
AATTGAAAAG
CTAATTCTTG
AAGACGAAAG
GGCCTCGTGA
TACGCCTATT
TTTATAGGTT
AATGTCATGA
TAATAATGGT
TTCTTAGACG
TCAGGTGGCA
CTTTTCGGGG
AAATGTGCGC
GGAACCCCTA
TTTGTTTATT
TTTCTAAATA
CATTCAAATA
TGTATCCGCT
CATGAGACAA
TAACCCTGAT
AAATGCTTCA
ATAATATTGA
AAAAGGAAGA
GTATGAGTAT
TCAACATTTC
CGTGTCGCCC
TTATTCCCTT
TTTTGCGGCA
TTTTGCCTTC
CTGTTTTTGC
TCACCCAGAA
ACGCTGGTGA
AAGTAAAAGA
TGCTGAAGAT
CAGTTGGGTG
CACGAGTGGG
TTACATCGAA
CTGGATCTCA
ACAGCGGTAA
GATCCTTGAG
AGTTTTCGCC
CCGAAGAACG
TTTTCCAATG
ATGAGCACTT
TTAAAGTTCT
GCTATGTGGC
GCGGTATTAT
CCCGTATTGA
CGCCGGGCAA
GAGCAACTCG
GTCGCCGCAT
ACACTATTCT
CAGAATGACT
TGGTTGAGTA
CTCACCAGTC
ACAGAAAAGC
ATCTTACGGA
TGGCATGACA
GTAAGAGAAT
TATGCAGTGC
TGCCATAACC
ATGAGTGATA
ACACTGCGGC
CAACTTACTT
CTGACAACGA
TCGGAGGACC
GAAGGAGCTA
ACCGCTTTTT
TGCACAACAT
GGGGGATCAT
GTAACTCGCC
TTGATCGTTG
GGAACCGGAG
CTGAATGAAG
CCATACCAAA
CGACGAGCGT
GACACCACGA
TGCCTGTAGC
AATGGCAACA
ACGTTGCGCA
AACTATTAAC
TGGCGAACTA
CTTACTCTAG
CTTCCCGGCA
ACAATTAATA
GACTGGATGG
AGGCGGATAA
AGTTGCAGGA
CCACTTCTGC
GCTCGGCCCT
TCCGGCTGGC
TGGTTTATTG
CTGATAAATC
TGGAGCCGGT
GAGCGTGGGT
CTCGCGGTAT
CATTGCAGCA
CTGGGGCCAG
ATGGTAAGCC
CTCCCGTATC
GTAGTTATCT
ACACGACGGG
GAGTCAGGCA
ACTATGGATG
AACGAAATAG
ACAGATCGCT
GAGATAGGTG
CCTCACTGAT
TAAGCATTGG
TAACTGTCAG
ACCAAGTTTA
CTCATATATA
CTTTAGATTG
ATTTAAAACT
TCATTTTTAA
TTTAAAAGGA
TCTAGGTGAA
GATCCTTTTT
GATAATCTCA
TGACCAAAAT
CCCTTAACGT
GAGTTTTCGT
TCCACTGAGC
GTCAGACCCC
GTAGAAAAGA
TCAAAGGATC
TTCTTGAGAT
CCTTTTTTTC
TGCGCGTAAT
CTGCTGCTTG
CAAACAAAAA
AACCACCGCT
ACCAGCGGTG
GTTTGTTTGC
CGGATCAAGA
GCTACCAACT
CTTTTTCCGA
AGGTAACTGG
CTTCAGCAGA
GCGCAGATAC
CAAATACTGT
TCTTCTAGTG
TAGCCGTAGT
TAGGCCACCA
CTTCAAGAAC
TCTGTAGCAC
CGCCTACATA
CCTCGCTCTG
CTAATCCTGT
TACCAGTGGC
TGCTGCCAGT
GGCGATAAGT
CGTGTCTTAC
CGGGTTGGAC
TCAAGACGAT
AGTTACCGGA
TAAGGCGCAG
CGGTCGGGCT
GAACGGGGGG
TTCGTGCACA
CAGCCCAGCT
TGGAGCGAAC
GACCTACACC
GAACTGAGAT
ACCTACAGCG
TGAGCTATGA
GAAAGCGCCA
CGCTTCCCGA
AGGGAGAAAG
GCGGACAGGT
ATCCGGTAAG
CGGCAGGGTC
GGAACAGGAG
AGCGCACGAG
GGAGCTTCCA
GGGGGAAACG
CCTGGTATCT
TTATAGTCCT
GTCGGGTTTC
GCCACCTCTG
ACTTGAGCGT
CGATTTTTGT
GATGCTCGTC
AGGGGGGCGG
AGCCTATGGA
AAAACGCCAG
CAACGCGGCC
TTTTTACGGT
TCCTGGCCTT
TTGCTGGCCT
TTTGCTCACA
TGTTCTTTCC
TGCGTTATCC
CCTGATTCTG
TGGATAACCG
TATTACCGCC
TTTGAGTGAG
CTGATACCGC
TCGCCGCAGC
CGAACGACCG
AGCGCAGCGA
GTCAGTGAGC
GAGGAAGCGG
AAGAGCGCCC
AATACGCAAA
CCGCCTCTCC
CCGCGCGTTG
GCCGATTCAT
TAATGCAGCT
GGCACGACAG
GTTTCCCGAC
TGGAAAGCGG
GCAGTGAGCG
CAACGCAATT
AATGTGAGTT
AGCTCACTCA
TTAGGCACCC
CAGGCTTTAC
ACTTTATGCT
TCCGGCTCGT
ATGTTGTGTG
GAATTGTGAG
CGGATAACAA
TTTCACACAG
GAAACAGCTA
TGACCATGAT
TACGCCAAGC
TTACCGCATC
AGGAAATTGT
AAGCGTTAAT
ATTTTGTTAA
AATTCGCGTT
AAATTTTTGT
TAAATCAGCT
CATTTTTTAA
CCAATAGGCC
GAAATCGGCA
AAATCCCTTA
TAAATCAAAA
GAATAGACCG
AGATAGGGTT
GAGTGTTGTT
CCAGTTTGGA
ACAAGAGTCC
ACTATTAAAG
AACGTGGACT
CCAACGTCAA
AGGGCGAAAA
ACCGTCTATC
AGGGCGATGG
CCCACTACGT
GAACCATCAC
CCTAATCAAG
TTTTTTGGGG
TCGAGGTGCC
GTAAAGCACT
AAATCGGAAC
CCTAAAGGGA
GCCCCCGATT
TAGAGCTTGA
CGGGGAAAGC
CGGCGAACGT
GGCGAGAAAG
GAAGGGAAGA
AAGCGAAAGG
AGCGGGCGCT
AGGGCGCTGG
CAAGTGTAGC
GGTCACGCTG
CGCGTAACCA
CCACACCCGC
CGCGCTTAAT
GCGCCGCTAC
AGGGCGCGTC
CATTCGCCAA
GCTTCCTGAA
ACGGAGAAAC
ATAAACAGGC
ATTGCTGGGA
TCACCCATAC
ATCACTCTGT
TTTGCCTGAC
CTTTTCCGGT
AATTTGAAAA
CAAACCCGGT
CTCGAAGCGG
AGATCCGGCG
ATAATTACCG
CAGAAATAAA
CCCATACACG
AGACGTAGAA
CCAGCCGCAC
ATGGCCGGAG
AAACTCCTGC
GAGAATTTCG
TAAACTCGCG
CGCATTGCAT
CTGTATTTCC
TAATGCGGCA
CTTCCAGGCC
TCGATCGAGA
CCGTTTATCC
ATTGCTTTTT
TGTTGTCTTT
TTCCCTCGTT
CACAGAAAGT
CTGAAGAAGC
TATAGTAGAA
CTATGAGCTT
TTTTTGTTTC
TGTTTTCCTT
TTTTTTTTTT
TTACCTCTGT
GGAAATTGTT
ACTCTCACAC
TCTTTAGTTC
GTTTGTTTGT
TTTGTTTATT
CCAATTATGA
CCGGTGACGA
AACGTGGTCG
ATGGTGGGTA
CCGCTTATGC
TCCCCTCCAT
TAGTTTCGAT
TATATAAAAA
GGCCAAATAT
TGTATTATTT
TCAAATGTCC
TATCATTATC
GTCTAACATC
TAATTTCTCT
TAAATTTTTT
CTCTTTCTTT
CCTATAACAC
CAATAGTGAA
AATCTTTTTT
TCTTCTATAT
CTACAAAAAC
TTTTTTTTTC
TATCAACCTC
GTTGATAAAT
TTTTTCTTTA
ACAATCGTTA
ATAATTAATT
AATTGGAAAA
TAACCATTTT
TTCTCTCTTT
TATACACACA
TTCAAAAGAA
AGAAAAAAAA
TATACCCCAG
CCTCGA