名称: | pAAV-CMV-Cas9-mCerulean-U6-TNFRSF21(human)-sgRNA1 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,腺相关病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCTGCAGGCA
GCTGCGCGCT
CGCTCGCTCA
CTGAGGCCGC
CCGGGCGTCG
GGCGACCTTT
GGTCGCCCGG
CCTCAGTGAG
CGAGCGAGCG
CGCAGAGAGG
GAGTGGCCAA
CTCCATCACT
AGGGGTTCCT
GCGGCCTCTA
GACTCGAGGC
GTTGACATTG
ATTATTGACT
AGTTATTAAT
AGTAATCAAT
TACGGGGTCA
TTAGTTCATA
GCCCATATAT
GGAGTTCCGC
GTTACATAAC
TTACGGTAAA
TGGCCCGCCT
GGCTGACCGC
CCAACGACCC
CCGCCCATTG
ACGTCAATAA
TGACGTATGT
TCCCATAGTA
ACGCCAATAG
GGACTTTCCA
TTGACGTCAA
TGGGTGGAGT
ATTTACGGTA
AACTGCCCAC
TTGGCAGTAC
ATCAAGTGTA
TCATATGCCA
AGTACGCCCC
CTATTGACGT
CAATGACGGT
AAATGGCCCG
CCTGGCATTA
TGCCCAGTAC
ATGACCTTAT
GGGACTTTCC
TACTTGGCAG
TACATCTACG
TATTAGTCAT
CGCTATTACC
ATGGTGATGC
GGTTTTGGCA
GTACATCAAT
GGGCGTGGAT
AGCGGTTTGA
CTCACGGGGA
TTTCCAAGTC
TCCACCCCAT
TGACGTCAAT
GGGAGTTTGT
TTTGGCACCA
AAATCAACGG
GACTTTCCAA
AATGTCGTAA
CAACTCCGCC
CCATTGACGC
AAATGGGCGG
TAGGCGTGTA
CGGTGGGAGG
TCTATATAAG
CAGAGCTCTC
TGGCTAACTA
CCGGTGCCAC
CATGGACTAT
AAGGACCACG
ACGGAGACTA
CAAGGATCAT
GATATTGATT
ACAAAGACGA
TGACGATAAG
ATGGCCCCAA
AGAAGAAGCG
GAAGGTCGGT
ATCCACGGAG
TCCCAGCAGC
CGACAAGAAG
TACAGCATCG
GCCTGGACAT
CGGCACCAAC
TCTGTGGGCT
GGGCCGTGAT
CACCGACGAG
TACAAGGTGC
CCAGCAAGAA
ATTCAAGGTG
CTGGGCAACA
CCGACCGGCA
CAGCATCAAG
AAGAACCTGA
TCGGAGCCCT
GCTGTTCGAC
AGCGGCGAAA
CAGCCGAGGC
CACCCGGCTG
AAGAGAACCG
CCAGAAGAAG
ATACACCAGA
CGGAAGAACC
GGATCTGCTA
TCTGCAAGAG
ATCTTCAGCA
ACGAGATGGC
CAAGGTGGAC
GACAGCTTCT
TCCACAGACT
GGAAGAGTCC
TTCCTGGTGG
AAGAGGATAA
GAAGCACGAG
CGGCACCCCA
TCTTCGGCAA
CATCGTGGAC
GAGGTGGCCT
ACCACGAGAA
GTACCCCACC
ATCTACCACC
TGAGAAAGAA
ACTGGTGGAC
AGCACCGACA
AGGCCGACCT
GCGGCTGATC
TATCTGGCCC
TGGCCCACAT
GATCAAGTTC
CGGGGCCACT
TCCTGATCGA
GGGCGACCTG
AACCCCGACA
ACAGCGACGT
GGACAAGCTG
TTCATCCAGC
TGGTGCAGAC
CTACAACCAG
CTGTTCGAGG
AAAACCCCAT
CAACGCCAGC
GGCGTGGACG
CCAAGGCCAT
CCTGTCTGCC
AGACTGAGCA
AGAGCAGACG
GCTGGAAAAT
CTGATCGCCC
AGCTGCCCGG
CGAGAAGAAG
AATGGCCTGT
TCGGAAACCT
GATTGCCCTG
AGCCTGGGCC
TGACCCCCAA
CTTCAAGAGC
AACTTCGACC
TGGCCGAGGA
TGCCAAACTG
CAGCTGAGCA
AGGACACCTA
CGACGACGAC
CTGGACAACC
TGCTGGCCCA
GATCGGCGAC
CAGTACGCCG
ACCTGTTTCT
GGCCGCCAAG
AACCTGTCCG
ACGCCATCCT
GCTGAGCGAC
ATCCTGAGAG
TGAACACCGA
GATCACCAAG
GCCCCCCTGA
GCGCCTCTAT
GATCAAGAGA
TACGACGAGC
ACCACCAGGA
CCTGACCCTG
CTGAAAGCTC
TCGTGCGGCA
GCAGCTGCCT
GAGAAGTACA
AAGAGATTTT
CTTCGACCAG
AGCAAGAACG
GCTACGCCGG
CTACATTGAC
GGCGGAGCCA
GCCAGGAAGA
GTTCTACAAG
TTCATCAAGC
CCATCCTGGA
AAAGATGGAC
GGCACCGAGG
AACTGCTCGT
GAAGCTGAAC
AGAGAGGACC
TGCTGCGGAA
GCAGCGGACC
TTCGACAACG
GCAGCATCCC
CCACCAGATC
CACCTGGGAG
AGCTGCACGC
CATTCTGCGG
CGGCAGGAAG
ATTTTTACCC
ATTCCTGAAG
GACAACCGGG
AAAAGATCGA
GAAGATCCTG
ACCTTCCGCA
TCCCCTACTA
CGTGGGCCCT
CTGGCCAGGG
GAAACAGCAG
ATTCGCCTGG
ATGACCAGAA
AGAGCGAGGA
AACCATCACC
CCCTGGAACT
TCGAGGAAGT
GGTGGACAAG
GGCGCTTCCG
CCCAGAGCTT
CATCGAGCGG
ATGACCAACT
TCGATAAGAA
CCTGCCCAAC
GAGAAGGTGC
TGCCCAAGCA
CAGCCTGCTG
TACGAGTACT
TCACCGTGTA
TAACGAGCTG
ACCAAAGTGA
AATACGTGAC
CGAGGGAATG
AGAAAGCCCG
CCTTCCTGAG
CGGCGAGCAG
AAAAAGGCCA
TCGTGGACCT
GCTGTTCAAG
ACCAACCGGA
AAGTGACCGT
GAAGCAGCTG
AAAGAGGACT
ACTTCAAGAA
AATCGAGTGC
TTCGACTCCG
TGGAAATCTC
CGGCGTGGAA
GATCGGTTCA
ACGCCTCCCT
GGGCACATAC
CACGATCTGC
TGAAAATTAT
CAAGGACAAG
GACTTCCTGG
ACAATGAGGA
AAACGAGGAC
ATTCTGGAAG
ATATCGTGCT
GACCCTGACA
CTGTTTGAGG
ACAGAGAGAT
GATCGAGGAA
CGGCTGAAAA
CCTATGCCCA
CCTGTTCGAC
GACAAAGTGA
TGAAGCAGCT
GAAGCGGCGG
AGATACACCG
GCTGGGGCAG
GCTGAGCCGG
AAGCTGATCA
ACGGCATCCG
GGACAAGCAG
TCCGGCAAGA
CAATCCTGGA
TTTCCTGAAG
TCCGACGGCT
TCGCCAACAG
AAACTTCATG
CAGCTGATCC
ACGACGACAG
CCTGACCTTT
AAAGAGGACA
TCCAGAAAGC
CCAGGTGTCC
GGCCAGGGCG
ATAGCCTGCA
CGAGCACATT
GCCAATCTGG
CCGGCAGCCC
CGCCATTAAG
AAGGGCATCC
TGCAGACAGT
GAAGGTGGTG
GACGAGCTCG
TGAAAGTGAT
GGGCCGGCAC
AAGCCCGAGA
ACATCGTGAT
CGAAATGGCC
AGAGAGAACC
AGACCACCCA
GAAGGGACAG
AAGAACAGCC
GCGAGAGAAT
GAAGCGGATC
GAAGAGGGCA
TCAAAGAGCT
GGGCAGCCAG
ATCCTGAAAG
AACACCCCGT
GGAAAACACC
CAGCTGCAGA
ACGAGAAGCT
GTACCTGTAC
TACCTGCAGA
ATGGGCGGGA
TATGTACGTG
GACCAGGAAC
TGGACATCAA
CCGGCTGTCC
GACTACGATG
TGGACCATAT
CGTGCCTCAG
AGCTTTCTGA
AGGACGACTC
CATCGACAAC
AAGGTGCTGA
CCAGAAGCGA
CAAGAACCGG
GGCAAGAGCG
ACAACGTGCC
CTCCGAAGAG
GTCGTGAAGA
AGATGAAGAA
CTACTGGCGG
CAGCTGCTGA
ACGCCAAGCT
GATTACCCAG
AGAAAGTTCG
ACAATCTGAC
CAAGGCCGAG
AGAGGCGGCC
TGAGCGAACT
GGATAAGGCC
GGCTTCATCA
AGAGACAGCT
GGTGGAAACC
CGGCAGATCA
CAAAGCACGT
GGCACAGATC
CTGGACTCCC
GGATGAACAC
TAAGTACGAC
GAGAATGACA
AGCTGATCCG
GGAAGTGAAA
GTGATCACCC
TGAAGTCCAA
GCTGGTGTCC
GATTTCCGGA
AGGATTTCCA
GTTTTACAAA
GTGCGCGAGA
TCAACAACTA
CCACCACGCC
CACGACGCCT
ACCTGAACGC
CGTCGTGGGA
ACCGCCCTGA
TCAAAAAGTA
CCCTAAGCTG
GAAAGCGAGT
TCGTGTACGG
CGACTACAAG
GTGTACGACG
TGCGGAAGAT
GATCGCCAAG
AGCGAGCAGG
AAATCGGCAA
GGCTACCGCC
AAGTACTTCT
TCTACAGCAA
CATCATGAAC
TTTTTCAAGA
CCGAGATTAC
CCTGGCCAAC
GGCGAGATCC
GGAAGCGGCC
TCTGATCGAG
ACAAACGGCG
AAACCGGGGA
GATCGTGTGG
GATAAGGGCC
GGGATTTTGC
CACCGTGCGG
AAAGTGCTGA
GCATGCCCCA
AGTGAATATC
GTGAAAAAGA
CCGAGGTGCA
GACAGGCGGC
TTCAGCAAAG
AGTCTATCCT
GCCCAAGAGG
AACAGCGATA
AGCTGATCGC
CAGAAAGAAG
GACTGGGACC
CTAAGAAGTA
CGGCGGCTTC
GACAGCCCCA
CCGTGGCCTA
TTCTGTGCTG
GTGGTGGCCA
AAGTGGAAAA
GGGCAAGTCC
AAGAAACTGA
AGAGTGTGAA
AGAGCTGCTG
GGGATCACCA
TCATGGAAAG
AAGCAGCTTC
GAGAAGAATC
CCATCGACTT
TCTGGAAGCC
AAGGGCTACA
AAGAAGTGAA
AAAGGACCTG
ATCATCAAGC
TGCCTAAGTA
CTCCCTGTTC
GAGCTGGAAA
ACGGCCGGAA
GAGAATGCTG
GCCTCTGCCG
GCGAACTGCA
GAAGGGAAAC
GAACTGGCCC
TGCCCTCCAA
ATATGTGAAC
TTCCTGTACC
TGGCCAGCCA
CTATGAGAAG
CTGAAGGGCT
CCCCCGAGGA
TAATGAGCAG
AAACAGCTGT
TTGTGGAACA
GCACAAGCAC
TACCTGGACG
AGATCATCGA
GCAGATCAGC
GAGTTCTCCA
AGAGAGTGAT
CCTGGCCGAC
GCTAATCTGG
ACAAAGTGCT
GTCCGCCTAC
AACAAGCACC
GGGATAAGCC
CATCAGAGAG
CAGGCCGAGA
ATATCATCCA
CCTGTTTACC
CTGACCAATC
TGGGAGCCCC
TGCCGCCTTC
AAGTACTTTG
ACACCACCAT
CGACCGGAAG
AGGTACACCA
GCACCAAAGA
GGTGCTGGAC
GCCACCCTGA
TCCACCAGAG
CATCACCGGC
CTGTACGAGA
CACGGATCGA
CCTGTCTCAG
CTGGGAGGCG
ACAAAAGGCC
GGCGGCCACG
AAAAAGGCCG
GCCAGGCAAA
AAAGAAAAAG
GAGTTCGGCA
GTGGAGAGGG
CAGAGGAAGT
CTGCTAACAT
GCGGTGACGT
CGAGGAGAAT
CCTGGCCCAA
TGGTGAGCAA
GGGCGAGGAG
CTGTTCACCG
GGGTGGTGCC
CATCCTGGTC
GAGCTGGACG
GCGACGTAAA
CGGCCACAAG
TTCAGCGTGT
CCGGCGAGGG
CGAGGGCGAT
GCCACCTACG
GCAAGCTGAC
CCTGAAGTTC
ATCTGCACCA
CCGGCAAGCT
GCCCGTGCCC
TGGCCCACCC
TCGTGACCAC
CCTGACCTGG
GGCGTGCAGT
GCTTCGCCCG
CTACCCCGAC
CACATGAAGC
AGCACGACTT
CTTCAAGTCC
GCCATGCCCG
AAGGCTACGT
CCAGGAGCGC
ACCATCTTCT
TCAAGGACGA
CGGCAACTAC
AAGACCCGCG
CCGAGGTGAA
GTTCGAGGGC
GACACCCTGG
TGAACCGCAT
CGAGCTGAAG
GGCATCGACT
TCAAGGAGGA
CGGCAACATC
CTGGGGCACA
AGCTGGAGTA
CAACGCCATC
AGCGACAACG
TCTATATCAC
CGCCGACAAG
CAGAAGAACG
GCATCAAGGC
CAACTTCAAG
ATCCGCCACA
ACATCGAGGA
CGGCAGCGTG
CAGCTCGCCG
ACCACTACCA
GCAGAACACC
CCCATCGGCG
ACGGCCCCGT
GCTGCTGCCC
GACAACCACT
ACCTGAGCAC
CCAGTCCGCC
CTGAGCAAAG
ACCCCAACGA
GAAGCGCGAT
CACATGGTCC
TGCTGGAGTT
CGTGACCGCC
GCCGGGATCA
CTCTCGGCAT
GGACGAGCTG
TACAAGTGAA
TTCCTAGAGC
TCGCTGATCA
GCCTCGACTG
TGCCTTCTAG
TTGCCAGCCA
TCTGTTGTTT
GCCCCTCCCC
CGTGCCTTCC
TTGACCCTGG
AAGGTGCCAC
TCCCACTGTC
CTTTCCTAAT
AAAATGAGGA
AATTGCATCG
CATTGTCTGA
GTAGGTGTCA
TTCTATTCTG
GGGGGTGGGG
TGGGGCAGGA
CAGCAAGGGG
GAGGATTGGG
AAGAGAATAG
CAGGCATGCT
GGGGAGGTAC
CTGAGGGCCT
ATTTCCCATG
ATTCCTTCAT
ATTTGCATAT
ACGATACAAG
GCTGTTAGAG
AGATAATTGG
AATTAATTTG
ACTGTAAACA
CAAAGATATT
AGTACAAAAT
ACGTGACGTA
GAAAGTAATA
ATTTCTTGGG
TAGTTTGCAG
TTTTAAAATT
ATGTTTTAAA
ATGGACTATC
ATATGCTTAC
CGTAACTTGA
AAGTATTTCG
ATTTCTTGGC
TTTATATATC
TTGTGGAAAG
GACGAAACAC
CGAAGGGAGC
CCGCGATCAT
CGGTTTTAGT
ACTCTGGAAA
CAGAATCTAC
TAAAACAAGG
CAAAATGCCG
TGTTTATCTC
GTCAACTTGT
TGGCGAGATT
TTTGCGGCCG
CAGGAACCCC
TAGTGATGGA
GTTGGCCACT
CCCTCTCTGC
GCGCTCGCTC
GCTCACTGAG
GCCGGGCGAC
CAAAGGTCGC
CCGACGCCCG
GGCTTTGCCC
GGGCGGCCTC
AGTGAGCGAG
CGAGCGCGCA
GCTGCCTGCA
GGGGCGCCTG
ATGCGGTATT
TTCTCCTTAC
GCATCTGTGC
GGTATTTCAC
ACCGCATACG
TCAAAGCAAC
CATAGTACGC
GCCCTGTAGC
GGCGCATTAA
GCGCGGCGGG
TGTGGTGGTT
ACGCGCAGCG
TGACCGCTAC
ACTTGCCAGC
GCCCTAGCGC
CCGCTCCTTT
CGCTTTCTTC
CCTTCCTTTC
TCGCCACGTT
CGCCGGCTTT
CCCCGTCAAG
CTCTAAATCG
GGGGCTCCCT
TTAGGGTTCC
GATTTAGTGC
TTTACGGCAC
CTCGACCCCA
AAAAACTTGA
TTTGGGTGAT
GGTTCACGTA
GTGGGCCATC
GCCCTGATAG
ACGGTTTTTC
GCCCTTTGAC
GTTGGAGTCC
ACGTTCTTTA
ATAGTGGACT
CTTGTTCCAA
ACTGGAACAA
CACTCAACCC
TATCTCGGGC
TATTCTTTTG
ATTTATAAGG
GATTTTGCCG
ATTTCGGCCT
ATTGGTTAAA
AAATGAGCTG
ATTTAACAAA
AATTTAACGC
GAATTTTAAC
AAAATATTAA
CGTTTACAAT
TTTATGGTGC
ACTCTCAGTA
CAATCTGCTC
TGATGCCGCA
TAGTTAAGCC
AGCCCCGACA
CCCGCCAACA
CCCGCTGACG
CGCCCTGACG
GGCTTGTCTG
CTCCCGGCAT
CCGCTTACAG
ACAAGCTGTG
ACCGTCTCCG
GGAGCTGCAT
GTGTCAGAGG
TTTTCACCGT
CATCACCGAA
ACGCGCGAGA
CGAAAGGGCC
TCGTGATACG
CCTATTTTTA
TAGGTTAATG
TCATGATAAT
AATGGTTTCT
TAGACGTCAG
GTGGCACTTT
TCGGGGAAAT
GTGCGCGGAA
CCCCTATTTG
TTTATTTTTC
TAAATACATT
CAAATATGTA
TCCGCTCATG
AGACAATAAC
CCTGATAAAT
GCTTCAATAA
TATTGAAAAA
GGAAGAGTAT
GAGTATTCAA
CATTTCCGTG
TCGCCCTTAT
TCCCTTTTTT
GCGGCATTTT
GCCTTCCTGT
TTTTGCTCAC
CCAGAAACGC
TGGTGAAAGT
AAAAGATGCT
GAAGATCAGT
TGGGTGCACG
AGTGGGTTAC
ATCGAACTGG
ATCTCAACAG
CGGTAAGATC
CTTGAGAGTT
TTCGCCCCGA
AGAACGTTTT
CCAATGATGA
GCACTTTTAA
AGTTCTGCTA
TGTGGCGCGG
TATTATCCCG
TATTGACGCC
GGGCAAGAGC
AACTCGGTCG
CCGCATACAC
TATTCTCAGA
ATGACTTGGT
TGAGTACTCA
CCAGTCACAG
AAAAGCATCT
TACGGATGGC
ATGACAGTAA
GAGAATTATG
CAGTGCTGCC
ATAACCATGA
GTGATAACAC
TGCGGCCAAC
TTACTTCTGA
CAACGATCGG
AGGACCGAAG
GAGCTAACCG
CTTTTTTGCA
CAACATGGGG
GATCATGTAA
CTCGCCTTGA
TCGTTGGGAA
CCGGAGCTGA
ATGAAGCCAT
ACCAAACGAC
GAGCGTGACA
CCACGATGCC
TGTAGCAATG
GCAACAACGT
TGCGCAAACT
ATTAACTGGC
GAACTACTTA
CTCTAGCTTC
CCGGCAACAA
TTAATAGACT
GGATGGAGGC
GGATAAAGTT
GCAGGACCAC
TTCTGCGCTC
GGCCCTTCCG
GCTGGCTGGT
TTATTGCTGA
TAAATCTGGA
GCCGGTGAGC
GTGGAAGCCG
CGGTATCATT
GCAGCACTGG
GGCCAGATGG
TAAGCCCTCC
CGTATCGTAG
TTATCTACAC
GACGGGGAGT
CAGGCAACTA
TGGATGAACG
AAATAGACAG
ATCGCTGAGA
TAGGTGCCTC
ACTGATTAAG
CATTGGTAAC
TGTCAGACCA
AGTTTACTCA
TATATACTTT
AGATTGATTT
AAAACTTCAT
TTTTAATTTA
AAAGGATCTA
GGTGAAGATC
CTTTTTGATA
ATCTCATGAC
CAAAATCCCT
TAACGTGAGT
TTTCGTTCCA
CTGAGCGTCA
GACCCCGTAG
AAAAGATCAA
AGGATCTTCT
TGAGATCCTT
TTTTTCTGCG
CGTAATCTGC
TGCTTGCAAA
CAAAAAAACC
ACCGCTACCA
GCGGTGGTTT
GTTTGCCGGA
TCAAGAGCTA
CCAACTCTTT
TTCCGAAGGT
AACTGGCTTC
AGCAGAGCGC
AGATACCAAA
TACTGTCCTT
CTAGTGTAGC
CGTAGTTAGG
CCACCACTTC
AAGAACTCTG
TAGCACCGCC
TACATACCTC
GCTCTGCTAA
TCCTGTTACC
AGTGGCTGCT
GCCAGTGGCG
ATAAGTCGTG
TCTTACCGGG
TTGGACTCAA
GACGATAGTT
ACCGGATAAG
GCGCAGCGGT
CGGGCTGAAC
GGGGGGTTCG
TGCACACAGC
CCAGCTTGGA
GCGAACGACC
TACACCGAAC
TGAGATACCT
ACAGCGTGAG
CTATGAGAAA
GCGCCACGCT
TCCCGAAGGG
AGAAAGGCGG
ACAGGTATCC
GGTAAGCGGC
AGGGTCGGAA
CAGGAGAGCG
CACGAGGGAG
CTTCCAGGGG
GAAACGCCTG
GTATCTTTAT
AGTCCTGTCG
GGTTTCGCCA
CCTCTGACTT
GAGCGTCGAT
TTTTGTGATG
CTCGTCAGGG
GGGCGGAGCC
TATGGAAAAA
CGCCAGCAAC
GCGGCCTTTT
TACGGTTCCT
GGCCTTTTGC
TGGCCTTTTG
CTCACATGT