名称: | pDsRed1-N1-CLOCK(human) |
---|---|
别称: | NM_001267843.2;bHLHe8; KAT13D |
基因长: | 2541bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Kan |
筛选标记: | Neo/G418 |
荧光标记: | 红色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
反向引物: | Sv40-polyA-R:GAAATTTGTGATGCTATTGC |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTGG
TTTAGTGAAC
CGTCAGATCC
GCTAGCGCTA
CCGGACTCAG
ATCTCGAGCT
CAAGCTTCGA
ATTCGCCACC
ATGTTGTTTA
CCGTAAGCTG
TAGTAAAATG
AGCTCGATTG
TTGACAGAGA
TGACAGTAGT
ATTTTTGATG
GGTTGGTGGA
AGAAGATGAC
AAGGACAAAG
CGAAAAGAGT
ATCTAGAAAC
AAATCTGAAA
AGAAACGTAG
AGATCAATTT
AATGTTCTCA
TTAAAGAACT
GGGATCCATG
CTTCCTGGTA
ATGCTAGAAA
GATGGACAAA
TCTACTGTTC
TGCAGAAAAG
CATTGATTTT
TTACGAAAAC
ATAAAGAAAT
CACTGCACAG
TCAGATGCTA
GTGAAATTCG
ACAGGACTGG
AAACCTACAT
TCCTTAGTAA
TGAAGAGTTT
ACACAATTAA
TGTTAGAGGC
TCTTGATGGT
TTTTTTTTAG
CAATCATGAC
AGATGGAAGC
ATAATATATG
TGTCTGAGAG
TGTAACTTCA
TTACTTGAAC
ATTTACCATC
TGATCTTGTG
GATCAAAGTA
TATTTAATTT
TATCCCAGAA
GGGGAACATT
CAGAGGTTTA
TAAAATACTC
TCTACTCATC
TGCTGGAAAG
TGATTCATTA
ACCCCAGAAT
ATTTAAAATC
AAAAAATCAG
TTAGAATTCT
GTTGTCACAT
GCTGCGAGGA
ACAATAGACC
CAAAGGAGCC
ATCTACCTAT
GAATATGTAA
AATTTATAGG
AAATTTCAAA
TCTTTAAACA
GTGTATCCTC
TTCAGCACAC
AATGGTTTTG
AAGGAACTAT
ACAACGCACA
CATAGGCCAT
CTTATGAAGA
TAGAGTTTGT
TTTGTAGCTA
CTGTCAGGTT
AGCTACACCT
CAGTTCATCA
AGGAAATGTG
CACTGTTGAA
GAACCCAATG
AAGAGTTTAC
ATCTAGACAT
AGTTTAGAAT
GGAAGTTTCT
GTTTCTAGAT
CACAGGGCAC
CACCCATAAT
AGGGTATTTG
CCATTTGAAG
TTCTGGGAAC
ATCAGGCTAT
GATTACTATC
ATGTGGATGA
CCTAGAAAAT
TTGGCAAAAT
GTCATGAGCA
CTTAATGCAA
TATGGGAAAG
GCAAATCATG
TTATTATAGG
TTCCTGACTA
AGGGGCAACA
GTGGATTTGG
CTTCAGACTC
ATTATTATAT
CACTTACCAT
CAGTGGAATT
CAAGGCCAGA
GTTTATTGTT
TGTACTCACA
CTGTAGTAAG
TTATGCAGAA
GTTAGGGCTG
AAAGACGACG
AGAACTTGGC
ATTGAAGAGT
CTCTTCCTGA
GACAGCTGCT
GACAAAAGCC
AAGATTCTGG
GTCAGATAAT
CGTATAAACA
CAGTCAGTCT
CAAGGAAGCA
TTGGAAAGGT
TTGATCACAG
CCCAACCCCT
TCTGCCTCTT
CTCGGAGTTC
AAGAAAATCA
TCTCACACGG
CCGTCTCAGA
CCCTTCCTCA
ACACCAACCA
AGATCCCGAC
GGATACGAGC
ACTCCACCCA
GGCAGCATTT
ACCAGCTCAT
GAGAAGATGG
TGCAAAGAAG
GTCATCATTT
AGTAGTCAGT
CCATAAATTC
CCAGTCTGTT
GGTTCATCAT
TAACACAGCC
AGTGATGTCT
CAAGCTACAA
ATTTACCAAT
TCCACAAGGC
ATGTCCCAGT
TTCAGTTTTC
AGCTCAATTA
GGAGCCATGC
AACATCTGAA
AGACCAATTG
GAACAACGGA
CACGCATGAT
AGAAGCAAAT
ATTCATCGGC
AACAAGAAGA
ACTAAGAAAA
ATTCAAGAAC
AACTTCAGAT
GGTCCATGGT
CAGGGGCTGC
AGATGTTTTT
GCAACAATCA
AATCCTGGGT
TGAATTTTGG
TTCCGTTCAA
CTTTCTTCTG
GAAATTCATC
TAATATCCAG
CAACTTGCAC
CTATAAATAT
GCAAGGCCAA
GTTGTTCCTA
CTAACCAGAT
TCAAAGTGGA
ATGAATACTG
GACACATTGG
CACAACTCAG
CACATGATAC
AACAACAGAC
TTTACAGAGT
ACATCAACTC
AGAGTCAACA
AAATGTACTG
AGTGGGCACA
GTCAGCAAAC
ATCTCTACCC
AGTCAGACAC
AGAGCACTCT
TACAGCCCCA
CTGTATAACA
CTATGGTGAT
TTCTCAGCCT
GCAGCCGGAA
GCATGGTCCA
GATTCCATCT
AGTATGCCAC
AAAACAGCAC
CCAGAGTGCT
GCAGTAACTA
CATTCACTCA
GGACAGGCAG
ATAAGATTTT
CTCAAGGTCA
ACAACTTGTG
ACCAAATTAG
TGACTGCTCC
TGTAGCTTGT
GGGGCAGTCA
TGGTACCTAG
TACTATGCTT
ATGGGCCAGG
TGGTGACTGC
ATATCCTACT
TTTGCTACAC
AACAGCAACA
GTCACAGACA
TTGTCAGTAA
CGCAGCAGCA
GCAGCAGCAG
AGCTCCCAGG
AGCAGCAGCT
CACTTCAGTT
CAGCAACCAT
CTCAGGCTCA
GCTGACCCAG
CCACCGCAAC
AATTTTTACA
GACTTCTAGG
TTGCTCCATG
GGAATCCCTC
AACTCAACTC
ATTCTCTCTG
CTGCATTTCC
TCTACAACAG
AGCACCTTCC
CTCAGTCACA
TCACCAGCAA
CATCAGTCTC
AGCAACAGCA
GCAACTCAGC
CGGCACAGGA
CTGACAGCTT
GCCCGACCCT
TCCAAGGTTC
AACCACAGGG
CGGCGGAGGG
AGTATGGTGC
GCTCCTCCAA
GAACGTCATC
AAGGAGTTCA
TGCGCTTCAA
GGTGCGCATG
GAGGGCACCG
TGAACGGCCA
CGAGTTCGAG
ATCGAGGGCG
AGGGCGAGGG
CCGCCCCTAC
GAGGGCCACA
ACACCGTGAA
GCTGAAGGTG
ACCAAGGGCG
GCCCCCTGCC
CTTCGCCTGG
GACATCCTGT
CCCCCCAGTT
CCAGTACGGC
TCCAAGGTGT
ACGTGAAGCA
CCCCGCCGAC
ATCCCCGACT
ACAAGAAGCT
GTCCTTCCCC
GAGGGCTTCA
AGTGGGAGCG
CGTGATGAAC
TTCGAGGACG
GCGGCGTGGT
GACCGTGACC
CAGGACTCCT
CCCTGCAGGA
CGGCTGCTTC
ATCTACAAGG
TGAAGTTCAT
CGGCGTGAAC
TTCCCCTCCG
ACGGCCCCGT
AATGCAGAAG
AAGACCATGG
GCTGGGAGGC
CTCCACCGAG
CGCCTGTACC
CCCGCGACGG
CGTGCTGAAG
GGCGAGATCC
ACAAGGCCCT
GAAGCTGAAG
GACGGCGGCC
ACTACCTGGT
GGAGTTCAAG
TCCATCTACA
TGGCCAAGAA
GCCCGTGCAG
CTGCCCGGCT
ACTACTACGT
GGACTCCAAG
CTGGACATCA
CCTCCCACAA
CGAGGACTAC
ACCATCGTGG
AGCAGTACGA
GCGCACCGAG
GGCCGCCACC
ACCTGTTCCT
GTAGCGGCCG
CGACTCTAGA
TCATAATCAG
CCATACCACA
TTTGTAGAGG
TTTTACTTGC
TTTAAAAAAC
CTCCCACACC
TCCCCCTGAA
CCTGAAACAT
AAAATGAATG
CAATTGTTGT
TGTTAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTAAGGC
GTAAATTGTA
AGCGTTAATA
TTTTGTTAAA
ATTCGCGTTA
AATTTTTGTT
AAATCAGCTC
ATTTTTTAAC
CAATAGGCCG
AAATCGGCAA
AATCCCTTAT
AAATCAAAAG
AATAGACCGA
GATAGGGTTG
AGTGTTGTTC
CAGTTTGGAA
CAAGAGTCCA
CTATTAAAGA
ACGTGGACTC
CAACGTCAAA
GGGCGAAAAA
CCGTCTATCA
GGGCGATGGC
CCACTACGTG
AACCATCACC
CTAATCAAGT
TTTTTGGGGT
CGAGGTGCCG
TAAAGCACTA
AATCGGAACC
CTAAAGGGAG
CCCCCGATTT
AGAGCTTGAC
GGGGAAAGCC
GGCGAACGTG
GCGAGAAAGG
AAGGGAAGAA
AGCGAAAGGA
GCGGGCGCTA
GGGCGCTGGC
AAGTGTAGCG
GTCACGCTGC
GCGTAACCAC
CACACCCGCC
GCGCTTAATG
CGCCGCTACA
GGGCGCGTCA
GGTGGCACTT
TTCGGGGAAA
TGTGCGCGGA
ACCCCTATTT
GTTTATTTTT
CTAAATACAT
TCAAATATGT
ATCCGCTCAT
GAGACAATAA
CCCTGATAAA
TGCTTCAATA
ATATTGAAAA
AGGAAGAGTC
CTGAGGCGGA
AAGAACCAGC
TGTGGAATGT
GTGTCAGTTA
GGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCAGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCATAGTC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGCT
TTTGCAAAGA
TCGATCAAGA
GACAGGATGA
GGATCGTTTC
GCATGATTGA
ACAAGATGGA
TTGCACGCAG
GTTCTCCGGC
CGCTTGGGTG
GAGAGGCTAT
TCGGCTATGA
CTGGGCACAA
CAGACAATCG
GCTGCTCTGA
TGCCGCCGTG
TTCCGGCTGT
CAGCGCAGGG
GCGCCCGGTT
CTTTTTGTCA
AGACCGACCT
GTCCGGTGCC
CTGAATGAAC
TGCAAGACGA
GGCAGCGCGG
CTATCGTGGC
TGGCCACGAC
GGGCGTTCCT
TGCGCAGCTG
TGCTCGACGT
TGTCACTGAA
GCGGGAAGGG
ACTGGCTGCT
ATTGGGCGAA
GTGCCGGGGC
AGGATCTCCT
GTCATCTCAC
CTTGCTCCTG
CCGAGAAAGT
ATCCATCATG
GCTGATGCAA
TGCGGCGGCT
GCATACGCTT
GATCCGGCTA
CCTGCCCATT
CGACCACCAA
GCGAAACATC
GCATCGAGCG
AGCACGTACT
CGGATGGAAG
CCGGTCTTGT
CGATCAGGAT
GATCTGGACG
AAGAGCATCA
GGGGCTCGCG
CCAGCCGAAC
TGTTCGCCAG
GCTCAAGGCG
AGCATGCCCG
ACGGCGAGGA
TCTCGTCGTG
ACCCATGGCG
ATGCCTGCTT
GCCGAATATC
ATGGTGGAAA
ATGGCCGCTT
TTCTGGATTC
ATCGACTGTG
GCCGGCTGGG
TGTGGCGGAC
CGCTATCAGG
ACATAGCGTT
GGCTACCCGT
GATATTGCTG
AAGAGCTTGG
CGGCGAATGG
GCTGACCGCT
TCCTCGTGCT
TTACGGTATC
GCCGCTCCCG
ATTCGCAGCG
CATCGCCTTC
TATCGCCTTC
TTGACGAGTT
CTTCTGAGCG
GGACTCTGGG
GTTCGAAATG
ACCGACCAAG
CGACGCCCAA
CCTGCCATCA
CGAGATTTCG
ATTCCACCGC
CGCCTTCTAT
GAAAGGTTGG
GCTTCGGAAT
CGTTTTCCGG
GACGCCGGCT
GGATGATCCT
CCAGCGCGGG
GATCTCATGC
TGGAGTTCTT
CGCCCACCCT
AGGGGGAGGC
TAACTGAAAC
ACGGAAGGAG
ACAATACCGG
AAGGAACCCG
CGCTATGACG
GCAATAAAAA
GACAGAATAA
AACGCACGGT
GTTGGGTCGT
TTGTTCATAA
ACGCGGGGTT
CGGTCCCAGG
GCTGGCACTC
TGTCGATACC
CCACCGAGAC
CCCATTGGGG
CCAATACGCC
CGCGTTTCTT
CCTTTTCCCC
ACCCCACCCC
CCAAGTTCGG
GTGAAGGCCC
AGGGCTCGCA
GCCAACGTCG
GGGCGGCAGG
CCCTGCCATA
GCCTCAGGTT
ACTCATATAT
ACTTTAGATT
GATTTAAAAC
TTCATTTTTA
ATTTAAAAGG
ATCTAGGTGA
AGATCCTTTT
TGATAATCTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TCCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGTTCTTTC
CTGCGTTATC
CCCTGATTCT
GTGGATAACC
GTATTACCGC
CATGCAT