收藏网址 | 中文

pDsRed1-N1-CLOCK(human)

产品货号:P45305

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pDsRed1-N1-CLOCK(human)
别称: NM_001267843.2;bHLHe8; KAT13D
基因长: 2541bp
质粒宿主: 哺乳细胞
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Kan
筛选标记: Neo/G418
荧光标记: 红色
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
反向引物: Sv40-polyA-R:GAAATTTGTGATGCTATTGC
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

TAGTTATTAA

TAGTAATCAA

TTACGGGGTC

ATTAGTTCAT

AGCCCATATA

TGGAGTTCCG

61

CGTTACATAA

CTTACGGTAA

ATGGCCCGCC

TGGCTGACCG

CCCAACGACC

CCCGCCCATT

121

GACGTCAATA

ATGACGTATG

TTCCCATAGT

AACGCCAATA

GGGACTTTCC

ATTGACGTCA

181

ATGGGTGGAG

TATTTACGGT

AAACTGCCCA

CTTGGCAGTA

CATCAAGTGT

ATCATATGCC

241

AAGTACGCCC

CCTATTGACG

TCAATGACGG

TAAATGGCCC

GCCTGGCATT

ATGCCCAGTA

301

CATGACCTTA

TGGGACTTTC

CTACTTGGCA

GTACATCTAC

GTATTAGTCA

TCGCTATTAC

361

CATGGTGATG

CGGTTTTGGC

AGTACATCAA

TGGGCGTGGA

TAGCGGTTTG

ACTCACGGGG

421

ATTTCCAAGT

CTCCACCCCA

TTGACGTCAA

TGGGAGTTTG

TTTTGGCACC

AAAATCAACG

481

GGACTTTCCA

AAATGTCGTA

ACAACTCCGC

CCCATTGACG

CAAATGGGCG

GTAGGCGTGT

541

ACGGTGGGAG

GTCTATATAA

GCAGAGCTGG

TTTAGTGAAC

CGTCAGATCC

GCTAGCGCTA

601

CCGGACTCAG

ATCTCGAGCT

CAAGCTTCGA

ATTCGCCACC

ATGTTGTTTA

CCGTAAGCTG

661

TAGTAAAATG

AGCTCGATTG

TTGACAGAGA

TGACAGTAGT

ATTTTTGATG

GGTTGGTGGA

721

AGAAGATGAC

AAGGACAAAG

CGAAAAGAGT

ATCTAGAAAC

AAATCTGAAA

AGAAACGTAG

781

AGATCAATTT

AATGTTCTCA

TTAAAGAACT

GGGATCCATG

CTTCCTGGTA

ATGCTAGAAA

841

GATGGACAAA

TCTACTGTTC

TGCAGAAAAG

CATTGATTTT

TTACGAAAAC

ATAAAGAAAT

901

CACTGCACAG

TCAGATGCTA

GTGAAATTCG

ACAGGACTGG

AAACCTACAT

TCCTTAGTAA

961

TGAAGAGTTT

ACACAATTAA

TGTTAGAGGC

TCTTGATGGT

TTTTTTTTAG

CAATCATGAC

1021

AGATGGAAGC

ATAATATATG

TGTCTGAGAG

TGTAACTTCA

TTACTTGAAC

ATTTACCATC

1081

TGATCTTGTG

GATCAAAGTA

TATTTAATTT

TATCCCAGAA

GGGGAACATT

CAGAGGTTTA

1141

TAAAATACTC

TCTACTCATC

TGCTGGAAAG

TGATTCATTA

ACCCCAGAAT

ATTTAAAATC

1201

AAAAAATCAG

TTAGAATTCT

GTTGTCACAT

GCTGCGAGGA

ACAATAGACC

CAAAGGAGCC

1261

ATCTACCTAT

GAATATGTAA

AATTTATAGG

AAATTTCAAA

TCTTTAAACA

GTGTATCCTC

1321

TTCAGCACAC

AATGGTTTTG

AAGGAACTAT

ACAACGCACA

CATAGGCCAT

CTTATGAAGA

1381

TAGAGTTTGT

TTTGTAGCTA

CTGTCAGGTT

AGCTACACCT

CAGTTCATCA

AGGAAATGTG

1441

CACTGTTGAA

GAACCCAATG

AAGAGTTTAC

ATCTAGACAT

AGTTTAGAAT

GGAAGTTTCT

1501

GTTTCTAGAT

CACAGGGCAC

CACCCATAAT

AGGGTATTTG

CCATTTGAAG

TTCTGGGAAC

1561

ATCAGGCTAT

GATTACTATC

ATGTGGATGA

CCTAGAAAAT

TTGGCAAAAT

GTCATGAGCA

1621

CTTAATGCAA

TATGGGAAAG

GCAAATCATG

TTATTATAGG

TTCCTGACTA

AGGGGCAACA

1681

GTGGATTTGG

CTTCAGACTC

ATTATTATAT

CACTTACCAT

CAGTGGAATT

CAAGGCCAGA

1741

GTTTATTGTT

TGTACTCACA

CTGTAGTAAG

TTATGCAGAA

GTTAGGGCTG

AAAGACGACG

1801

AGAACTTGGC

ATTGAAGAGT

CTCTTCCTGA

GACAGCTGCT

GACAAAAGCC

AAGATTCTGG

1861

GTCAGATAAT

CGTATAAACA

CAGTCAGTCT

CAAGGAAGCA

TTGGAAAGGT

TTGATCACAG

1921

CCCAACCCCT

TCTGCCTCTT

CTCGGAGTTC

AAGAAAATCA

TCTCACACGG

CCGTCTCAGA

1981

CCCTTCCTCA

ACACCAACCA

AGATCCCGAC

GGATACGAGC

ACTCCACCCA

GGCAGCATTT

2041

ACCAGCTCAT

GAGAAGATGG

TGCAAAGAAG

GTCATCATTT

AGTAGTCAGT

CCATAAATTC

2101

CCAGTCTGTT

GGTTCATCAT

TAACACAGCC

AGTGATGTCT

CAAGCTACAA

ATTTACCAAT

2161

TCCACAAGGC

ATGTCCCAGT

TTCAGTTTTC

AGCTCAATTA

GGAGCCATGC

AACATCTGAA

2221

AGACCAATTG

GAACAACGGA

CACGCATGAT

AGAAGCAAAT

ATTCATCGGC

AACAAGAAGA

2281

ACTAAGAAAA

ATTCAAGAAC

AACTTCAGAT

GGTCCATGGT

CAGGGGCTGC

AGATGTTTTT

2341

GCAACAATCA

AATCCTGGGT

TGAATTTTGG

TTCCGTTCAA

CTTTCTTCTG

GAAATTCATC

2401

TAATATCCAG

CAACTTGCAC

CTATAAATAT

GCAAGGCCAA

GTTGTTCCTA

CTAACCAGAT

2461

TCAAAGTGGA

ATGAATACTG

GACACATTGG

CACAACTCAG

CACATGATAC

AACAACAGAC

2521

TTTACAGAGT

ACATCAACTC

AGAGTCAACA

AAATGTACTG

AGTGGGCACA

GTCAGCAAAC

2581

ATCTCTACCC

AGTCAGACAC

AGAGCACTCT

TACAGCCCCA

CTGTATAACA

CTATGGTGAT

2641

TTCTCAGCCT

GCAGCCGGAA

GCATGGTCCA

GATTCCATCT

AGTATGCCAC

AAAACAGCAC

2701

CCAGAGTGCT

GCAGTAACTA

CATTCACTCA

GGACAGGCAG

ATAAGATTTT

CTCAAGGTCA

2761

ACAACTTGTG

ACCAAATTAG

TGACTGCTCC

TGTAGCTTGT

GGGGCAGTCA

TGGTACCTAG

2821

TACTATGCTT

ATGGGCCAGG

TGGTGACTGC

ATATCCTACT

TTTGCTACAC

AACAGCAACA

2881

GTCACAGACA

TTGTCAGTAA

CGCAGCAGCA

GCAGCAGCAG

AGCTCCCAGG

AGCAGCAGCT

2941

CACTTCAGTT

CAGCAACCAT

CTCAGGCTCA

GCTGACCCAG

CCACCGCAAC

AATTTTTACA

3001

GACTTCTAGG

TTGCTCCATG

GGAATCCCTC

AACTCAACTC

ATTCTCTCTG

CTGCATTTCC

3061

TCTACAACAG

AGCACCTTCC

CTCAGTCACA

TCACCAGCAA

CATCAGTCTC

AGCAACAGCA

3121

GCAACTCAGC

CGGCACAGGA

CTGACAGCTT

GCCCGACCCT

TCCAAGGTTC

AACCACAGGG

3181

CGGCGGAGGG

AGTATGGTGC

GCTCCTCCAA

GAACGTCATC

AAGGAGTTCA

TGCGCTTCAA

3241

GGTGCGCATG

GAGGGCACCG

TGAACGGCCA

CGAGTTCGAG

ATCGAGGGCG

AGGGCGAGGG

3301

CCGCCCCTAC

GAGGGCCACA

ACACCGTGAA

GCTGAAGGTG

ACCAAGGGCG

GCCCCCTGCC

3361

CTTCGCCTGG

GACATCCTGT

CCCCCCAGTT

CCAGTACGGC

TCCAAGGTGT

ACGTGAAGCA

3421

CCCCGCCGAC

ATCCCCGACT

ACAAGAAGCT

GTCCTTCCCC

GAGGGCTTCA

AGTGGGAGCG

3481

CGTGATGAAC

TTCGAGGACG

GCGGCGTGGT

GACCGTGACC

CAGGACTCCT

CCCTGCAGGA

3541

CGGCTGCTTC

ATCTACAAGG

TGAAGTTCAT

CGGCGTGAAC

TTCCCCTCCG

ACGGCCCCGT

3601

AATGCAGAAG

AAGACCATGG

GCTGGGAGGC

CTCCACCGAG

CGCCTGTACC

CCCGCGACGG

3661

CGTGCTGAAG

GGCGAGATCC

ACAAGGCCCT

GAAGCTGAAG

GACGGCGGCC

ACTACCTGGT

3721

GGAGTTCAAG

TCCATCTACA

TGGCCAAGAA

GCCCGTGCAG

CTGCCCGGCT

ACTACTACGT

3781

GGACTCCAAG

CTGGACATCA

CCTCCCACAA

CGAGGACTAC

ACCATCGTGG

AGCAGTACGA

3841

GCGCACCGAG

GGCCGCCACC

ACCTGTTCCT

GTAGCGGCCG

CGACTCTAGA

TCATAATCAG

3901

CCATACCACA

TTTGTAGAGG

TTTTACTTGC

TTTAAAAAAC

CTCCCACACC

TCCCCCTGAA

3961

CCTGAAACAT

AAAATGAATG

CAATTGTTGT

TGTTAACTTG

TTTATTGCAG

CTTATAATGG

4021

TTACAAATAA

AGCAATAGCA

TCACAAATTT

CACAAATAAA

GCATTTTTTT

CACTGCATTC

4081

TAGTTGTGGT

TTGTCCAAAC

TCATCAATGT

ATCTTAAGGC

GTAAATTGTA

AGCGTTAATA

4141

TTTTGTTAAA

ATTCGCGTTA

AATTTTTGTT

AAATCAGCTC

ATTTTTTAAC

CAATAGGCCG

4201

AAATCGGCAA

AATCCCTTAT

AAATCAAAAG

AATAGACCGA

GATAGGGTTG

AGTGTTGTTC

4261

CAGTTTGGAA

CAAGAGTCCA

CTATTAAAGA

ACGTGGACTC

CAACGTCAAA

GGGCGAAAAA

4321

CCGTCTATCA

GGGCGATGGC

CCACTACGTG

AACCATCACC

CTAATCAAGT

TTTTTGGGGT

4381

CGAGGTGCCG

TAAAGCACTA

AATCGGAACC

CTAAAGGGAG

CCCCCGATTT

AGAGCTTGAC

4441

GGGGAAAGCC

GGCGAACGTG

GCGAGAAAGG

AAGGGAAGAA

AGCGAAAGGA

GCGGGCGCTA

4501

GGGCGCTGGC

AAGTGTAGCG

GTCACGCTGC

GCGTAACCAC

CACACCCGCC

GCGCTTAATG

4561

CGCCGCTACA

GGGCGCGTCA

GGTGGCACTT

TTCGGGGAAA

TGTGCGCGGA

ACCCCTATTT

4621

GTTTATTTTT

CTAAATACAT

TCAAATATGT

ATCCGCTCAT

GAGACAATAA

CCCTGATAAA

4681

TGCTTCAATA

ATATTGAAAA

AGGAAGAGTC

CTGAGGCGGA

AAGAACCAGC

TGTGGAATGT

4741

GTGTCAGTTA

GGGTGTGGAA

AGTCCCCAGG

CTCCCCAGCA

GGCAGAAGTA

TGCAAAGCAT

4801

GCATCTCAAT

TAGTCAGCAA

CCAGGTGTGG

AAAGTCCCCA

GGCTCCCCAG

CAGGCAGAAG

4861

TATGCAAAGC

ATGCATCTCA

ATTAGTCAGC

AACCATAGTC

CCGCCCCTAA

CTCCGCCCAT

4921

CCCGCCCCTA

ACTCCGCCCA

GTTCCGCCCA

TTCTCCGCCC

CATGGCTGAC

TAATTTTTTT

4981

TATTTATGCA

GAGGCCGAGG

CCGCCTCGGC

CTCTGAGCTA

TTCCAGAAGT

AGTGAGGAGG

5041

CTTTTTTGGA

GGCCTAGGCT

TTTGCAAAGA

TCGATCAAGA

GACAGGATGA

GGATCGTTTC

5101

GCATGATTGA

ACAAGATGGA

TTGCACGCAG

GTTCTCCGGC

CGCTTGGGTG

GAGAGGCTAT

5161

TCGGCTATGA

CTGGGCACAA

CAGACAATCG

GCTGCTCTGA

TGCCGCCGTG

TTCCGGCTGT

5221

CAGCGCAGGG

GCGCCCGGTT

CTTTTTGTCA

AGACCGACCT

GTCCGGTGCC

CTGAATGAAC

5281

TGCAAGACGA

GGCAGCGCGG

CTATCGTGGC

TGGCCACGAC

GGGCGTTCCT

TGCGCAGCTG

5341

TGCTCGACGT

TGTCACTGAA

GCGGGAAGGG

ACTGGCTGCT

ATTGGGCGAA

GTGCCGGGGC

5401

AGGATCTCCT

GTCATCTCAC

CTTGCTCCTG

CCGAGAAAGT

ATCCATCATG

GCTGATGCAA

5461

TGCGGCGGCT

GCATACGCTT

GATCCGGCTA

CCTGCCCATT

CGACCACCAA

GCGAAACATC

5521

GCATCGAGCG

AGCACGTACT

CGGATGGAAG

CCGGTCTTGT

CGATCAGGAT

GATCTGGACG

5581

AAGAGCATCA

GGGGCTCGCG

CCAGCCGAAC

TGTTCGCCAG

GCTCAAGGCG

AGCATGCCCG

5641

ACGGCGAGGA

TCTCGTCGTG

ACCCATGGCG

ATGCCTGCTT

GCCGAATATC

ATGGTGGAAA

5701

ATGGCCGCTT

TTCTGGATTC

ATCGACTGTG

GCCGGCTGGG

TGTGGCGGAC

CGCTATCAGG

5761

ACATAGCGTT

GGCTACCCGT

GATATTGCTG

AAGAGCTTGG

CGGCGAATGG

GCTGACCGCT

5821

TCCTCGTGCT

TTACGGTATC

GCCGCTCCCG

ATTCGCAGCG

CATCGCCTTC

TATCGCCTTC

5881

TTGACGAGTT

CTTCTGAGCG

GGACTCTGGG

GTTCGAAATG

ACCGACCAAG

CGACGCCCAA

5941

CCTGCCATCA

CGAGATTTCG

ATTCCACCGC

CGCCTTCTAT

GAAAGGTTGG

GCTTCGGAAT

6001

CGTTTTCCGG

GACGCCGGCT

GGATGATCCT

CCAGCGCGGG

GATCTCATGC

TGGAGTTCTT

6061

CGCCCACCCT

AGGGGGAGGC

TAACTGAAAC

ACGGAAGGAG

ACAATACCGG

AAGGAACCCG

6121

CGCTATGACG

GCAATAAAAA

GACAGAATAA

AACGCACGGT

GTTGGGTCGT

TTGTTCATAA

6181

ACGCGGGGTT

CGGTCCCAGG

GCTGGCACTC

TGTCGATACC

CCACCGAGAC

CCCATTGGGG

6241

CCAATACGCC

CGCGTTTCTT

CCTTTTCCCC

ACCCCACCCC

CCAAGTTCGG

GTGAAGGCCC

6301

AGGGCTCGCA

GCCAACGTCG

GGGCGGCAGG

CCCTGCCATA

GCCTCAGGTT

ACTCATATAT

6361

ACTTTAGATT

GATTTAAAAC

TTCATTTTTA

ATTTAAAAGG

ATCTAGGTGA

AGATCCTTTT

6421

TGATAATCTC

ATGACCAAAA

TCCCTTAACG

TGAGTTTTCG

TTCCACTGAG

CGTCAGACCC

6481

CGTAGAAAAG

ATCAAAGGAT

CTTCTTGAGA

TCCTTTTTTT

CTGCGCGTAA

TCTGCTGCTT

6541

GCAAACAAAA

AAACCACCGC

TACCAGCGGT

GGTTTGTTTG

CCGGATCAAG

AGCTACCAAC

6601

TCTTTTTCCG

AAGGTAACTG

GCTTCAGCAG

AGCGCAGATA

CCAAATACTG

TCCTTCTAGT

6661

GTAGCCGTAG

TTAGGCCACC

ACTTCAAGAA

CTCTGTAGCA

CCGCCTACAT

ACCTCGCTCT

6721

GCTAATCCTG

TTACCAGTGG

CTGCTGCCAG

TGGCGATAAG

TCGTGTCTTA

CCGGGTTGGA

6781

CTCAAGACGA

TAGTTACCGG

ATAAGGCGCA

GCGGTCGGGC

TGAACGGGGG

GTTCGTGCAC

6841

ACAGCCCAGC

TTGGAGCGAA

CGACCTACAC

CGAACTGAGA

TACCTACAGC

GTGAGCTATG

6901

AGAAAGCGCC

ACGCTTCCCG

AAGGGAGAAA

GGCGGACAGG

TATCCGGTAA

GCGGCAGGGT

6961

CGGAACAGGA

GAGCGCACGA

GGGAGCTTCC

AGGGGGAAAC

GCCTGGTATC

TTTATAGTCC

7021

TGTCGGGTTT

CGCCACCTCT

GACTTGAGCG

TCGATTTTTG

TGATGCTCGT

CAGGGGGGCG

7081

GAGCCTATGG

AAAAACGCCA

GCAACGCGGC

CTTTTTACGG

TTCCTGGCCT

TTTGCTGGCC

7141

TTTTGCTCAC

ATGTTCTTTC

CTGCGTTATC

CCCTGATTCT

GTGGATAACC

GTATTACCGC

7201

CATGCAT

武汉网监电子标识 Copyright © 武汉淼灵生物科技有限公司版权所有/鄂公网安备42010702000282号 鄂ICP备14012402号-1
访客数:2972992
1.215295s