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pLV3-CMV-Sp3(mouse-gc-opt)-3×FLAG-CopGFP-Puro

产品货号:P45301

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pLV3-CMV-Sp3(mouse-gc-opt)-3×FLAG-CopGFP-Puro
别称: NM_001018042.3;D130027J01Rik
基因长: 2352bp
质粒宿主: 哺乳细胞,慢病毒
质粒用途: 蛋白表达
片段类型: ORF
片段物种: 小鼠
原核抗性: Amp
筛选标记: Puro
荧光标记: 绿色
启动子: CMV
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

ACGCGTGTAG

TCTTATGCAA

TACTCTTGTA

GTCTTGCAAC

ATGGTAACGA

TGAGTTAGCA

61

ACATGCCTTA

CAAGGAGAGA

AAAAGCACCG

TGCATGCCGA

TTGGTGGAAG

TAAGGTGGTA

121

CGATCGTGCC

TTATTAGGAA

GGCAACAGAC

GGGTCTGACA

TGGATTGGAC

GAACCACTGA

181

ATTGCCGCAT

TGCAGAGATA

TTGTATTTAA

GTGCCTAGCT

CGATACAATA

AACGGGTCTC

241

TCTGGTTAGA

CCAGATCTGA

GCCTGGGAGC

TCTCTGGCTA

ACTAGGGAAC

CCACTGCTTA

301

AGCCTCAATA

AAGCTTGCCT

TGAGTGCTTC

AAGTAGTGTG

TGCCCGTCTG

TTGTGTGACT

361

CTGGTAACTA

GAGATCCCTC

AGACCCTTTT

AGTCAGTGTG

GAAAATCTCT

AGCAGTGGCG

421

CCCGAACAGG

GACCTGAAAG

CGAAAGGGAA

ACCAGAGCTC

TCTCGACGCA

GGACTCGGCT

481

TGCTGAAGCG

CGCACGGCAA

GAGGCGAGGG

GCGGCGACTG

GTGAGTACGC

CAAAAATTTT

541

GACTAGCGGA

GGCTAGAAGG

AGAGAGATGG

GTGCGAGAGC

GTCAGTATTA

AGCGGGGGAG

601

AATTAGATCG

CGATGGGAAA

AAATTCGGTT

AAGGCCAGGG

GGAAAGAAAA

AATATAAATT

661

AAAACATATA

GTATGGGCAA

GCAGGGAGCT

AGAACGATTC

GCAGTTAATC

CTGGCCTGTT

721

AGAAACATCA

GAAGGCTGTA

GACAAATACT

GGGACAGCTA

CAACCATCCC

TTCAGACAGG

781

ATCAGAAGAA

CTTAGATCAT

TATATAATAC

AGTAGCAACC

CTCTATTGTG

TGCATCAAAG

841

GATAGAGATA

AAAGACACCA

AGGAAGCTTT

AGACAAGATA

GAGGAAGAGC

AAAACAAAAG

901

TAAGACCACC

GCACAGCAAG

CGGCCACTGA

TCTTCAGACC

TGGAGGAGGA

GATATGAGGG

961

ACAATTGGAG

AAGTGAATTA

TATAAATATA

AAGTAGTAAA

AATTGAACCA

TTAGGAGTAG

1021

CACCCACCAA

GGCAAAGAGA

AGAGTGGTGC

AGAGAGAAAA

AAGAGCAGTG

GGAATAGGAG

1081

CTTTGTTCCT

TGGGTTCTTG

GGAGCAGCAG

GAAGCACTAT

GGGCGCAGCC

TCAATGACGC

1141

TGACGGTACA

GGCCAGACAA

TTATTGTCTG

GTATAGTGCA

GCAGCAGAAC

AATTTGCTGA

1201

GGGCTATTGA

GGCGCAACAG

CATCTGTTGC

AACTCACAGT

CTGGGGCATC

AAGCAGCTCC

1261

AGGCAAGAAT

CCTGGCTGTG

GAAAGATACC

TAAAGGATCA

ACAGCTCCTG

GGGATTTGGG

1321

GTTGCTCTGG

AAAACTCATT

TGCACCACTG

CTGTGCCTTG

GAATGCTAGT

TGGAGTAATA

1381

AATCTCTGGA

ACAGATTGGA

ATCACACGAC

CTGGATGGAG

TGGGACAGAG

AAATTAACAA

1441

TTACACAAGC

TTAATACACT

CCTTAATTGA

AGAATCGCAA

AACCAGCAAG

AAAAGAATGA

1501

ACAAGAATTA

TTGGAATTAG

ATAAATGGGC

AAGTTTGTGG

AATTGGTTTA

ACATAACAAA

1561

TTGGCTGTGG

TATATAAAAT

TATTCATAAT

GATAGTAGGA

GGCTTGGTAG

GTTTAAGAAT

1621

AGTTTTTGCT

GTACTTTCTA

TAGTGAATAG

AGTTAGGCAG

GGATATTCAC

CATTATCGTT

1681

TCAGACCCAC

CTCCCAACCC

CGAGGGGACC

CGACAGGCCC

GAAGGAATAG

AAGAAGAAGG

1741

TGGAGAGAGA

GACAGAGACA

GATCCATTCG

ATTAGTGAAC

GGATCTCGAC

GGTATCGGTT

1801

AACTTTTAAA

AGAAAAGGGG

GGATTGGGGG

GTACAGTGCA

GGGGAAAGAA

TAGTAGACAT

1861

AATAGCAACA

GACATACAAA

CTAAAGAATT

ACAAAAACAA

ATTACAAAAA

TTCAAAATTT

1921

TATCGATACT

AGTATTATGC

CCAGTACATG

ACCTTATGGG

ACTTTCCTAC

TTGGCAGTAC

1981

ATCTACGTAT

TAGTCATCGC

TATTACCATG

GTGATGCGGT

TTTGGCAGTA

CATCAATGGG

2041

CGTGGATAGC

GGTTTGACTC

ACGGGGATTT

CCAAGTCTCC

ACCCCATTGA

CGTCAATGGG

2101

AGTTTGTTTT

GGCACCAAAA

TCAACGGGAC

TTTCCAAAAT

GTCGTAACAA

CTCCGCCCCA

2161

TTGACGCAAA

TGGGCGGTAG

GCGTGTACGG

TGGGAGGTTT

ATATAAGCAG

AGCTCGTTTA

2221

GTGAACCGTC

AGATCGCCTG

GAGACGCCAT

CCACGCTGTT

TTGACCTCCA

TAGAAGATTC

2281

TAGAGCTAGC

CTGATACGAA

CTCGGAATTC

GCCACCATGA

CCGCTCCTGA

GAAGCCTGTG

2341

AAGCAGGAGG

AGATGGCCGC

ACTGGATGTG

GATGGAGGAG

GAGGAGGCGG

AGGACATGGA

2401

GAATACCTGC

AACAGCAGCA

GCAGCAGCAG

CAGCAGCATG

GAAACGGAGC

AGCAGCAGCT

2461

GCCGCTCAGG

ATACACAGCC

TAGTCCTCTG

GCACTGCTGG

CTGCTACATG

CAGTAAGATC

2521

GGCCCCCCTT

CCCCTGGAGA

TGATGATGAA

GAGGCCGCTG

TGGCTGCCGC

TGCTGGAGTG

2581

CCTGCTGCTG

CTGCTGGAGC

TACAGGAGAT

TTGGCCTCTG

CCCAGCTGGG

AGGAGCCCCT

2641

AACAGATGGG

AAGTGCTGAG

CGCTACACCT

ACTACCATTA

AGGATGAAGC

CGGAAACCTG

2701

GTGCAGATTC

CTGGCGCTGC

TACAAGCTCC

GGACAGTATG

TGCTGCCTCT

GCAAAACCTG

2761

CAAAATCAGC

AGATTTTTTC

TGTGGCCCCC

GGCAGCGACA

GCTCTAACGG

AACAGTGTCT

2821

AACGTGCAGT

ATCAGGTGAT

CCCCCAGATC

CAGAGCACCG

ATGCTCAGCA

GGTGCAGATC

2881

GGATTTACCG

GCTCTTCCGA

TAACGGCGGA

ATCAACCAGG

AGAACAGCCA

GATTCAGATC

2941

ATCCCCGGCA

GCAACCAGAC

CCTGCTGGCT

TCTGGAACAC

CTCCAGCAAA

TATCCAGAAC

3001

CTGATCCCAC

AGACCGGCCA

GGTGCAGGTG

CAGGGAGTGG

CTATCGGAGG

AAGTAGCTTT

3061

CCAGGACAGA

CACAGGTGGT

GGCTAATGTG

CCCCTGGGAC

TGCCTGGAAA

TATCACCTTC

3121

GTGCCTATCA

ACAGTGTGGA

TCTGGATAGT

CTGGGGCTGA

GCGGATCTTC

CCAGACTATG

3181

ACAGCAGGAA

TCAATGCCGA

CGGACACCTG

ATCAATACCG

GCCAGGCAAT

GGACTCCAGC

3241

GACAATAGCG

AGAGGACCGG

AGAGAGGGTG

AGTCCTGATG

TGAACGAGAC

AAACGCCGAC

3301

ACCGACCTGT

TCGTGCCTAC

TTCTTCCAGC

AGCCAGCTGC

CAGTGACCAT

CGATAGCACC

3361

GGAATCCTGC

AACAGAACAC

AAACAGCCTG

ACCACCACCA

GCGGCCAGGT

GCATTCTTCT

3421

GACCTGCAAG

GCAACTACAT

CCAGAGCCCC

GTGTCCGAAG

AAACCCAGGC

TCAGAACATC

3481

CAGGTGAGCA

CCGCTCAGCC

TGTGGTGCAG

CATCTGCAAC

TGCAAGACTC

CCAGCAGCCT

3541

ACCAGCCAGG

CTCAGATTGT

GCAGGGAATT

ACCCCCCAGA

CCATCCACGG

TGTGCAGGCT

3601

TCTGGACAGA

ACATCTCTCA

GCAGGCCCTG

CAAAACCTGC

AACTGCAACT

GAACCCCGGC

3661

ACCTTCCTGA

TCCAGGCTCA

GACAGTGACC

CCTAGCGGAC

AGATCACATG

GCAGACCTTC

3721

CAGGTGCAGG

GCGTGCAGAA

TCTGCAAAAC

CTGCAAATCC

AGAACACCGC

CGCCCAGCAG

3781

ATTACCCTGA

CACCTGTGCA

GACCCTGACC

CTGGGACAGG

TGGCTGCTGG

AGGAGCTTTG

3841

ACAAGCACCC

CTGTGAGCCT

GAGCACCGGA

CAGCTGCCTA

ATCTGCAAAC

AGTGACCGTG

3901

AACTCTATCG

ACTCCACCGG

CATCCAGCTG

CATCCAGGAG

AAAATGCTGA

CTCCCCTGCC

3961

GACATTAGAA

TTAAGGAAGA

AGAGCCTGAC

CCCGAAGAAT

GGCAGCTGTC

TGGAGACTCC

4021

ACCCTGAACA

CCAACGACCT

GACCCACCTG

AGGGTGCAGG

TGGTGGATGA

AGAAGGAGAT

4081

CAACAGCACC

AGGAGGGAAA

GAGGCTGAGG

AGAGTGGCTT

GCACATGCCC

TAACTGCAAA

4141

GAGGGCGGCG

GAAGAGGAAC

CAACCTGGGA

AAAAAGAAGC

AGCACATCTG

CCACATCCCT

4201

GGCTGCGGCA

AGGTGTACGG

CAAGACCAGC

CACCTGAGGG

CTCATCTGAG

GTGGCACAGC

4261

GGCGAGAGGC

CCTTCATCTG

CAACTGGATG

TTCTGCGGCA

AGAGGTTCAC

CAGGAGCGAC

4321

GAGCTGCAAA

GGCACAGGAG

GACCCACACC

GGCGAGAAGA

AGTTCGTGTG

CCCTGAGTGC

4381

AGCAAGAGGT

TCATGAGGAG

CGACCACCTG

GCCAAGCACA

TCAAGACCCA

CCAGAACAAG

4441

AAGGTGATCC

ACAGCAGCAG

CACCGTGCTG

GCCAGCGTGG

AGGCCGGCAG

GGACGACGCC

4501

CTGATCACCG

CCGGCGGCAC

CACCCTGATC

CTGGCCAACA

TCCAGCAGGG

CAGCGTGAGC

4561

GGCATCGGCA

CCGTGAACAC

CAGCGCCACC

AGCAACCAGG

ACATCCTGAC

CAACACCGAG

4621

ATCCCTCTGC

AACTGGTGAC

CGTGAGCGGC

AACGAGACAA

TGGAGGGATC

CGACTACAAA

4681

GACCATGACG

GTGATTATAA

AGATCATGAC

ATCGACTACA

AGGATGACGA

TGACAAGTAG

4741

CGGCCGCGAA

GGATCTGCGA

TCGCTCCGGT

GCCCGTCAGT

GGGCAGAGCG

CACATCGCCC

4801

ACAGTCCCCG

AGAAGTTGGG

GGGAGGGGTC

GGCAATTGAA

CGGGTGCCTA

GAGAAGGTGG

4861

CGCGGGGTAA

ACTGGGAAAG

TGATGTCGTG

TACTGGCTCC

GCCTTTTTCC

CGAGGGTGGG

4921

GGAGAACCGT

ATATAAGTGC

AGTAGTCGCC

GTGAACGTTC

TTTTTCGCAA

CGGGTTTGCC

4981

GCCAGAACAC

AGCTGAAGCT

TCGAGGGGCT

CGCATCTCTC

CTTCACGCGC

CCGCCGCCCT

5041

ACCTGAGGCC

GCCATCCACG

CCGGTTGAGT

CGCGTTCTGC

CGCCTCCCGC

CTGTGGTGCC

5101

TCCTGAACTG

CGTCCGCCGT

CTAGGTAAGT

TTAAAGCTCA

GGTCGAGACC

GGGCCTTTGT

5161

CCGGCGCTCC

CTTGGAGCCT

ACCTAGACTC

AGCCGGCTCT

CCACGCTTTG

CCTGACCCTG

5221

CTTGCTCAAC

TCTACGTCTT

TGTTTCGTTT

TCTGTTCTGC

GCCGTTACAG

ATCCAAGCTG

5281

TGACCGGCGC

CTACGCTAGA

CGCCACCATG

GAGAGCGACG

AGAGCGGCCT

GCCCGCCATG

5341

GAGATCGAGT

GCCGCATCAC

CGGCACCCTG

AACGGCGTGG

AGTTCGAGCT

GGTGGGCGGC

5401

GGAGAGGGCA

CCCCCAAGCA

GGGCCGCATG

ACCAACAAGA

TGAAGAGCAC

CAAAGGCGCC

5461

CTGACCTTCA

GCCCCTACCT

GCTGAGCCAC

GTGATGGGCT

ACGGCTTCTA

CCACTTCGGC

5521

ACCTACCCCA

GCGGCTACGA

GAACCCCTTC

CTGCACGCCA

TCAACAACGG

CGGCTACACC

5581

AACACCCGCA

TCGAGAAGTA

CGAGGACGGC

GGCGTGCTGC

ACGTGAGCTT

CAGCTACCGC

5641

TACGAGGCCG

GCCGCGTGAT

CGGCGACTTC

AAGGTGGTGG

GCACCGGCTT

CCCCGAGGAC

5701

AGCGTGATCT

TCACCGACAA

GATCATCCGC

AGCAACGCCA

CCGTGGAGCA

CCTGCACCCC

5761

ATGGGCGATA

ACGTGCTGGT

GGGCAGCTTC

GCCCGCACCT

TCAGCCTGCG

CGACGGCGGC

5821

TACTACAGCT

TCGTGGTGGA

CAGCCACATG

CACTTCAAGA

GCGCCATCCA

CCCCAGCATC

5881

CTGCAGAACG

GGGGCCCCAT

GTTCGCCTTC

CGCCGCGTGG

AGGAGCTGCA

CAGCAACACC

5941

GAGCTGGGCA

TCGTGGAGTA

CCAGCACGCC

TTCAAGACCC

CCATCGCCTT

CGCCAGATCC

6001

CGCGCTCAGT

CGTCCAATTC

TGCCGTGGAC

GGCACCGCCG

GACCCGGCTC

CACCGGATCT

6061

CGCGAGGGCA

GAGGAAGTCT

TCTAACATGC

GGTGACGTGG

AGGAGAATCC

CGGCCCTATG

6121

ACCGAGTACA

AGCCCACGGT

GCGCCTCGCC

ACCCGCGACG

ACGTCCCCAG

GGCCGTACGC

6181

ACCCTCGCCG

CCGCGTTCGC

CGACTACCCC

GCCACGCGCC

ACACCGTCGA

TCCGGACCGC

6241

CACATCGAGC

GGGTCACCGA

GCTGCAAGAA

CTCTTCCTCA

CGCGCGTCGG

GCTCGACATC

6301

GGCAAGGTGT

GGGTCGCGGA

CGACGGCGCC

GCGGTGGCGG

TCTGGACCAC

GCCGGAGAGC

6361

GTCGAAGCGG

GGGCGGTGTT

CGCCGAGATC

GGCCCGCGCA

TGGCCGAGTT

GAGCGGTTCC

6421

CGGCTGGCCG

CGCAGCAACA

GATGGAAGGC

CTCCTGGCGC

CGCACCGGCC

CAAGGAGCCC

6481

GCGTGGTTCC

TGGCCACCGT

CGGCGTCTCG

CCCGACCACC

AGGGCAAGGG

TCTGGGCAGC

6541

GCCGTCGTGC

TCCCCGGAGT

GGAGGCGGCC

GAGCGCGCCG

GGGTGCCCGC

CTTCCTGGAG

6601

ACCTCCGCGC

CCCGCAACCT

CCCCTTCTAC

GAGCGGCTCG

GCTTCACCGT

CACCGCCGAC

6661

GTCGAGGTGC

CCGAAGGACC

GCGCACCTGG

TGCATGACCC

GCAAGCCCGG

TGCCTGAAAT

6721

CAACCTCTGG

ATTACAAAAT

TTGTGAAAGA

TTGACTGGTA

TTCTTAACTA

TGTTGCTCCT

6781

TTTACGCTAT

GTGGATACGC

TGCTTTAATG

CCTTTGTATC

ATGCTATTGC

TTCCCGTATG

6841

GCTTTCATTT

TCTCCTCCTT

GTATAAATCC

TGGTTGCTGT

CTCTTTATGA

GGAGTTGTGG

6901

CCCGTTGTCA

GGCAACGTGG

CGTGGTGTGC

ACTGTGTTTG

CTGACGCAAC

CCCCACTGGT

6961

TGGGGCATTG

CCACCACCTG

TCAGCTCCTT

TCCGGGACTT

TCGCTTTCCC

CCTCCCTATT

7021

GCCACGGCGG

AACTCATCGC

CGCCTGCCTT

GCCCGCTGCT

GGACAGGGGC

TCGGCTGTTG

7081

GGCACTGACA

ATTCCGTGGT

GTTGTCGGGG

AAGCTGACGT

CCTTTCCATG

GCTGCTCGCC

7141

TGTGTTGCCA

CCTGGATTCT

GCGCGGGACG

TCCTTCTGCT

ACGTCCCTTC

GGCCCTCAAT

7201

CCAGCGGACC

TTCCTTCCCG

CGGCCTGCTG

CCGGCTCTGC

GGCCTCTTCC

GCGTCTCCGC

7261

CTTCGCCCTC

AGACGAGTCG

GATCTCCCTT

TGGCCGCCTC

CCCGCCTGGT

ACCTTTAAGA

7321

CCAATGACTT

ACAAGGCAGC

TGTAGATCTT

AGCCACTTTT

TAAAAGAAAA

GGGGGGACTG

7381

GAAGGGCTAA

TTCACTCCCA

ACGAAAATAA

GATCTGCTTT

TTGCTTGTAC

TGGGTCTCTC

7441

TGGTTAGACC

AGATCTGAGC

CTGGGAGCTC

TCTGGCTAAC

TAGGGAACCC

ACTGCTTAAG

7501

CCTCAATAAA

GCTTGCCTTG

AGTGCTTCAA

GTAGTGTGTG

CCCGTCTGTT

GTGTGACTCT

7561

GGTAACTAGA

GATCCCTCAG

ACCCTTTTAG

TCAGTGTGGA

AAATCTCTAG

CAGTAGTAGT

7621

TCATGTCATC

TTATTATTCA

GTATTTATAA

CTTGCAAAGA

AATGAATATC

AGAGAGTGAG

7681

AGGAACTTGT

TTATTGCAGC

TTATAATGGT

TACAAATAAA

GCAATAGCAT

CACAAATTTC

7741

ACAAATAAAG

CATTTTTTTC

ACTGCATTCT

AGTTGTGGTT

TGTCCAAACT

CATCAATGTA

7801

TCTTATCATG

TCTGGCTCTA

GCTATCCCGC

CCCTAACTCC

GCCCAGTTCC

GCCCATTCTC

7861

CGCCCCATGG

CTGACTAATT

TTTTTTATTT

ATGCAGAGGC

CGAGGCCGCC

TCGGCCTCTG

7921

AGCTATTCCA

GAAGTAGTGA

GGAGGCTTTT

TTGGAGGCCT

AGACTTTTGC

AGAGACGGCC

7981

CAAATTCGTA

ATCATGGTCA

TAGCTGTTTC

CTGTGTGAAA

TTGTTATCCG

CTCACAATTC

8041

CACACAACAT

ACGAGCCGGA

AGCATAAAGT

GTAAAGCCTG

GGGTGCCTAA

TGAGTGAGCT

8101

AACTCACATT

AATTGCGTTG

CGCTCACTGC

CCGCTTTCCA

GTCGGGAAAC

CTGTCGTGCC

8161

AGCTGCATTA

ATGAATCGGC

CAACGCGCGG

GGAGAGGCGG

TTTGCGTATT

GGGCGCTCTT

8221

CCGCTTCCTC

GCTCACTGAC

TCGCTGCGCT

CGGTCGTTCG

GCTGCGGCGA

GCGGTATCAG

8281

CTCACTCAAA

GGCGGTAATA

CGGTTATCCA

CAGAATCAGG

GGATAACGCA

GGAAAGAACA

8341

TGTGAGCAAA

AGGCCAGCAA

AAGGCCAGGA

ACCGTAAAAA

GGCCGCGTTG

CTGGCGTTTT

8401

TCCATAGGCT

CCGCCCCCCT

GACGAGCATC

ACAAAAATCG

ACGCTCAAGT

CAGAGGTGGC

8461

GAAACCCGAC

AGGACTATAA

AGATACCAGG

CGTTTCCCCC

TGGAAGCTCC

CTCGTGCGCT

8521

CTCCTGTTCC

GACCCTGCCG

CTTACCGGAT

ACCTGTCCGC

CTTTCTCCCT

TCGGGAAGCG

8581

TGGCGCTTTC

TCATAGCTCA

CGCTGTAGGT

ATCTCAGTTC

GGTGTAGGTC

GTTCGCTCCA

8641

AGCTGGGCTG

TGTGCACGAA

CCCCCCGTTC

AGCCCGACCG

CTGCGCCTTA

TCCGGTAACT

8701

ATCGTCTTGA

GTCCAACCCG

GTAAGACACG

ACTTATCGCC

ACTGGCAGCA

GCCACTGGTA

8761

ACAGGATTAG

CAGAGCGAGG

TATGTAGGCG

GTGCTACAGA

GTTCTTGAAG

TGGTGGCCTA

8821

ACTACGGCTA

CACTAGAAGG

ACAGTATTTG

GTATCTGCGC

TCTGCTGAAG

CCAGTTACCT

8881

TCGGAAAAAG

AGTTGGTAGC

TCTTGATCCG

GCAAACAAAC

CACCGCTGGT

AGCGGTGGTT

8941

TTTTTGTTTG

CAAGCAGCAG

ATTACGCGCA

GAAAAAAAGG

ATCTCAAGAA

GATCCTTTGA

9001

TCTTTTCTAC

GGGGTCTGAC

GCTCAGTGGA

ACGAAAACTC

ACGTTAAGGG

ATTTTGGTCA

9061

TGAGATTATC

AAAAAGGATC

TTCACCTAGA

TCCTTTTAAA

TTAAAAATGA

AGTTTTAAAT

9121

CAATCTAAAG

TATATATGAG

TAAACTTGGT

CTGACAGTTA

CCAATGCTTA

ATCAGTGAGG

9181

CACCTATCTC

AGCGATCTGT

CTATTTCGTT

CATCCATAGT

TGCCTGACTC

CCCGTCGTGT

9241

AGATAACTAC

GATACGGGAG

GGCTTACCAT

CTGGCCCCAG

TGCTGCAATG

ATACCGCGAG

9301

ACCCACGCTC

ACCGGCTCCA

GATTTATCAG

CAATAAACCA

GCCAGCCGGA

AGGGCCGAGC

9361

GCAGAAGTGG

TCCTGCAACT

TTATCCGCCT

CCATCCAGTC

TATTAATTGT

TGCCGGGAAG

9421

CTAGAGTAAG

TAGTTCGCCA

GTTAATAGTT

TGCGCAACGT

TGTTGCCATT

GCTACAGGCA

9481

TCGTGGTGTC

ACGCTCGTCG

TTTGGTATGG

CTTCATTCAG

CTCCGGTTCC

CAACGATCAA

9541

GGCGAGTTAC

ATGATCCCCC

ATGTTGTGCA

AAAAAGCGGT

TAGCTCCTTC

GGTCCTCCGA

9601

TCGTTGTCAG

AAGTAAGTTG

GCCGCAGTGT

TATCACTCAT

GGTTATGGCA

GCACTGCATA

9661

ATTCTCTTAC

TGTCATGCCA

TCCGTAAGAT

GCTTTTCTGT

GACTGGTGAG

TACTCAACCA

9721

AGTCATTCTG

AGAATAGTGT

ATGCGGCGAC

CGAGTTGCTC

TTGCCCGGCG

TCAATACGGG

9781

ATAATACCGC

GCCACATAGC

AGAACTTTAA

AAGTGCTCAT

CATTGGAAAA

CGTTCTTCGG

9841

GGCGAAAACT

CTCAAGGATC

TTACCGCTGT

TGAGATCCAG

TTCGATGTAA

CCCACTCGTG

9901

CACCCAACTG

ATCTTCAGCA

TCTTTTACTT

TCACCAGCGT

TTCTGGGTGA

GCAAAAACAG

9961

GAAGGCAAAA

TGCCGCAAAA

AAGGGAATAA

GGGCGACACG

GAAATGTTGA

ATACTCATAC

10021

TCTTCCTTTT

TCAATATTAT

TGAAGCATTT

ATCAGGGTTA

TTGTCTCATG

AGCGGATACA

10081

TATTTGAATG

TATTTAGAAA

AATAAACAAA

TAGGGGTTCC

GCGCACATTT

CCCCGAAAAG

10141

TGCCACCTGA

CGTCTAAGAA

ACCATTATTA

TCATGACATT

AACCTATAAA

AATAGGCGTA

10201

TCACGAGGCC

CTTTCGTCTC

GCGCGTTTCG

GTGATGACGG

TGAAAACCTC

TGACACATGC

10261

AGCTCCCGGA

GACGGTCACA

GCTTGTCTGT

AAGCGGATGC

CGGGAGCAGA

CAAGCCCGTC

10321

AGGGCGCGTC

AGCGGGTGTT

GGCGGGTGTC

GGGGCTGGCT

TAACTATGCG

GCATCAGAGC

10381

AGATTGTACT

GAGAGTGCAC

CATATGCGGT

GTGAAATACC

GCACAGATGC

GTAAGGAGAA

10441

AATACCGCAT

CAGGCGCCAT

TCGCCATTCA

GGCTGCGCAA

CTGTTGGGAA

GGGCGATCGG

10501

TGCGGGCCTC

TTCGCTATTA

CGCCAGCTGG

CGAAAGGGGG

ATGTGCTGCA

AGGCGATTAA

10561

GTTGGGTAAC

GCCAGGGTTT

TCCCAGTCAC

GACGTTGTAA

AACGACGGCC

AGTGCCAAGC

10621

TG

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