名称: | pDsRed1-N1-SATB1(human) |
---|---|
别称: | NM_001131010.4 |
基因长: | 2292bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Kan |
筛选标记: | Neo/G418 |
荧光标记: | 红色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
反向引物: | Sv40-polyA-R:GAAATTTGTGATGCTATTGC |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTGG
TTTAGTGAAC
CGTCAGATCC
GCTAGCGCTA
CCGGACTCAG
ATCTCGAGCT
CAAGCTTCGA
ATTCGCCACC
ATGGATCATT
TGAACGAGGC
AACTCAGGGG
AAAGAACATT
CAGAAATGTC
TAACAATGTG
AGTGATCCGA
AGGGTCCACC
AGCCAAGATT
GCCCGCCTGG
AGCAGAACGG
GAGCCCGCTA
GGAAGAGGAA
GGCTTGGGAG
TACAGGTGCA
AAAATGCAGG
GAGTGCCTTT
AAAACACTCG
GGCCATCTGA
TGAAAACCAA
CCTTAGGAAA
GGAACCATGC
TGCCAGTTTT
CTGTGTGGTG
GAACATTATG
AAAACGCCAT
TGAATATGAT
TGCAAGGAGG
AGCATGCAGA
ATTTGTGCTG
GTGAGAAAGG
ATATGCTTTT
CAACCAGCTG
ATCGAAATGG
CATTGCTGTC
TCTAGGTTAT
TCACATAGCT
CTGCTGCCCA
GGCCAAAGGG
CTAATCCAGG
TTGGAAAGTG
GAATCCAGTT
CCACTGTCTT
ACGTGACAGA
TGCCCCTGAT
GCTACAGTAG
CAGATATGCT
TCAAGATGTG
TATCATGTGG
TCACATTGAA
AATTCAGTTA
CACAGTTGCC
CCAAACTAGA
AGACTTGCCT
CCCGAACAAT
GGTCGCACAC
CACAGTGAGG
AATGCTCTGA
AGGACTTACT
GAAAGATATG
AATCAGAGTT
CATTGGCCAA
GGAGTGCCCC
CTTTCACAGA
GTATGATTTC
TTCCATTGTG
AACAGTACTT
ACTATGCAAA
TGTCTCAGCA
GCAAAATGTC
AAGAATTTGG
AAGGTGGTAC
AAACATTTCA
AGAAGACAAA
AGATATGATG
GTTGAAATGG
ATAGTCTTTC
TGAGCTATCC
CAGCAAGGCG
CCAATCATGT
CAATTTTGGC
CAGCAACCAG
TTCCAGGGAA
CACAGCCGAG
CAGCCTCCAT
CCCCTGCGCA
GCTCTCCCAT
GGCAGCCAGC
CCTCTGTCCG
GACACCTCTT
CCAAACCTGC
ACCCTGGGCT
CGTATCAACA
CCTATCAGTC
CTCAATTGGT
CAACCAGCAG
CTGGTGATGG
CTCAGCTGCT
GAACCAGCAG
TATGCAGTGA
ATAGACTTTT
AGCCCAGCAG
TCCTTAAACC
AACAATACTT
GAACCACCCT
CCCCCTGTCA
GTAGATCTAT
GAATAAGCCT
TTGGAGCAAC
AGGTTTCGAC
CAACACAGAG
GTGTCTTCCG
AAATCTACCA
GTGGGTACGC
GATGAACTGA
AACGAGCAGG
AATCTCCCAG
GCGGTATTTG
CACGTGTGGC
TTTTAACAGA
ACTCAGGGCT
TGCTTTCAGA
AATCCTCCGA
AAGGAAGAGG
ACCCCAAGAC
TGCATCCCAG
TCTTTGCTGG
TAAACCTTCG
GGCTATGCAG
AATTTCTTGC
AGTTACCGGA
AGCTGAAAGA
GACCGAATAT
ACCAGGACGA
AAGGGAAAGG
AGCTTGAATG
CTGCCTCGGC
CATGGGTCCT
GCCCCCCTCA
TCAGCACACC
ACCCAGCCGT
CCTCCCCAGG
TGAAAACAGC
TACTATTGCC
ACTGAAAGGA
ATGGGAAACC
AGAGAACAAT
ACCATGAACA
TTAATGCTTC
CATTTATGAT
GAGATTCAGC
AGGAAATGAA
GCGTGCTAAA
GTGTCTCAAG
CACTGTTTGC
AAAGGTTGCA
GCAACCAAAA
GCCAGGGATG
GTTGTGCGAG
CTGTTACGCT
GGAAAGAAGA
TCCTTCTCCA
GAAAACAGAA
CCCTGTGGGA
GAACCTCTCC
ATGATCCGAA
GGTTCCTCAG
TCTTCCTCAG
CCAGAACGTG
ATGCCATTTA
TGAACAGGAG
AGCAACGCGG
TGCATCACCA
TGGCGACAGG
CCGCCCCACA
TTATCCATGT
TCCAGCAGAG
CAGATTCAGA
GCCCCTCTCC
CACCACCCTT
GGGAAAGGAG
AGTCTAGAGG
CGTTTTCTTA
CCAGGCCTGC
CGACCCCTGC
ACCATGGCTC
GGTGCTGCTC
CTCAGCAACA
GCAGCAGCAA
CAGCAACAGC
AGCAGCAGCA
GCAGCAGGCA
CCGCCGCCTC
CACAGCCACA
GCAGCAGCCA
CAGACAGGCC
CTCGGCTCCC
CCCACGGCAA
CCCACGGTGG
CCTCTCCAGC
AGAGTCAGAT
GAGGAAAACC
GACAGAAGAC
CCGGCCACGA
ACAAAAATTT
CAGTGGAAGC
CTTGGGAATC
CTCCAGAGTT
TCATACAAGA
CGTGGGCCTG
TACCCTGACG
AAGAGGCCAT
CCAGACTCTG
TCTGCCCAGC
TCGACCTTCC
CAAGTACACC
ATCATCAAGT
TCTTTCAGAA
CCAGCGGTAC
TATCTCAAGC
ACCACGGCAA
ACTGAAGGAC
AATTCCGGTT
TAGAGGTCGA
TGTGGCAGAA
TATAAAGAAG
AGGAGCTGCT
GAAGGATTTG
GAAGAGAGTG
TCCAAGATAA
AAATACTAAC
ACCCTTTTTT
CAGTGAAACT
AGAAGAAGAG
CTGTCAGTGG
AAGGAAACAC
AGACATTAAT
ACTGATTTGA
AAGACGGCGG
CGGAGGGAGT
ATGGTGCGCT
CCTCCAAGAA
CGTCATCAAG
GAGTTCATGC
GCTTCAAGGT
GCGCATGGAG
GGCACCGTGA
ACGGCCACGA
GTTCGAGATC
GAGGGCGAGG
GCGAGGGCCG
CCCCTACGAG
GGCCACAACA
CCGTGAAGCT
GAAGGTGACC
AAGGGCGGCC
CCCTGCCCTT
CGCCTGGGAC
ATCCTGTCCC
CCCAGTTCCA
GTACGGCTCC
AAGGTGTACG
TGAAGCACCC
CGCCGACATC
CCCGACTACA
AGAAGCTGTC
CTTCCCCGAG
GGCTTCAAGT
GGGAGCGCGT
GATGAACTTC
GAGGACGGCG
GCGTGGTGAC
CGTGACCCAG
GACTCCTCCC
TGCAGGACGG
CTGCTTCATC
TACAAGGTGA
AGTTCATCGG
CGTGAACTTC
CCCTCCGACG
GCCCCGTAAT
GCAGAAGAAG
ACCATGGGCT
GGGAGGCCTC
CACCGAGCGC
CTGTACCCCC
GCGACGGCGT
GCTGAAGGGC
GAGATCCACA
AGGCCCTGAA
GCTGAAGGAC
GGCGGCCACT
ACCTGGTGGA
GTTCAAGTCC
ATCTACATGG
CCAAGAAGCC
CGTGCAGCTG
CCCGGCTACT
ACTACGTGGA
CTCCAAGCTG
GACATCACCT
CCCACAACGA
GGACTACACC
ATCGTGGAGC
AGTACGAGCG
CACCGAGGGC
CGCCACCACC
TGTTCCTGTA
GCGGCCGCGA
CTCTAGATCA
TAATCAGCCA
TACCACATTT
GTAGAGGTTT
TACTTGCTTT
AAAAAACCTC
CCACACCTCC
CCCTGAACCT
GAAACATAAA
ATGAATGCAA
TTGTTGTTGT
TAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTAAGGCGTA
AATTGTAAGC
GTTAATATTT
TGTTAAAATT
CGCGTTAAAT
TTTTGTTAAA
TCAGCTCATT
TTTTAACCAA
TAGGCCGAAA
TCGGCAAAAT
CCCTTATAAA
TCAAAAGAAT
AGACCGAGAT
AGGGTTGAGT
GTTGTTCCAG
TTTGGAACAA
GAGTCCACTA
TTAAAGAACG
TGGACTCCAA
CGTCAAAGGG
CGAAAAACCG
TCTATCAGGG
CGATGGCCCA
CTACGTGAAC
CATCACCCTA
ATCAAGTTTT
TTGGGGTCGA
GGTGCCGTAA
AGCACTAAAT
CGGAACCCTA
AAGGGAGCCC
CCGATTTAGA
GCTTGACGGG
GAAAGCCGGC
GAACGTGGCG
AGAAAGGAAG
GGAAGAAAGC
GAAAGGAGCG
GGCGCTAGGG
CGCTGGCAAG
TGTAGCGGTC
ACGCTGCGCG
TAACCACCAC
ACCCGCCGCG
CTTAATGCGC
CGCTACAGGG
CGCGTCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTCCTG
AGGCGGAAAG
AACCAGCTGT
GGAATGTGTG
TCAGTTAGGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CATAGTCCCG
CCCCTAACTC
CGCCCATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCGGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGGCTTTT
GCAAAGATCG
ATCAAGAGAC
AGGATGAGGA
TCGTTTCGCA
TGATTGAACA
AGATGGATTG
CACGCAGGTT
CTCCGGCCGC
TTGGGTGGAG
AGGCTATTCG
GCTATGACTG
GGCACAACAG
ACAATCGGCT
GCTCTGATGC
CGCCGTGTTC
CGGCTGTCAG
CGCAGGGGCG
CCCGGTTCTT
TTTGTCAAGA
CCGACCTGTC
CGGTGCCCTG
AATGAACTGC
AAGACGAGGC
AGCGCGGCTA
TCGTGGCTGG
CCACGACGGG
CGTTCCTTGC
GCAGCTGTGC
TCGACGTTGT
CACTGAAGCG
GGAAGGGACT
GGCTGCTATT
GGGCGAAGTG
CCGGGGCAGG
ATCTCCTGTC
ATCTCACCTT
GCTCCTGCCG
AGAAAGTATC
CATCATGGCT
GATGCAATGC
GGCGGCTGCA
TACGCTTGAT
CCGGCTACCT
GCCCATTCGA
CCACCAAGCG
AAACATCGCA
TCGAGCGAGC
ACGTACTCGG
ATGGAAGCCG
GTCTTGTCGA
TCAGGATGAT
CTGGACGAAG
AGCATCAGGG
GCTCGCGCCA
GCCGAACTGT
TCGCCAGGCT
CAAGGCGAGC
ATGCCCGACG
GCGAGGATCT
CGTCGTGACC
CATGGCGATG
CCTGCTTGCC
GAATATCATG
GTGGAAAATG
GCCGCTTTTC
TGGATTCATC
GACTGTGGCC
GGCTGGGTGT
GGCGGACCGC
TATCAGGACA
TAGCGTTGGC
TACCCGTGAT
ATTGCTGAAG
AGCTTGGCGG
CGAATGGGCT
GACCGCTTCC
TCGTGCTTTA
CGGTATCGCC
GCTCCCGATT
CGCAGCGCAT
CGCCTTCTAT
CGCCTTCTTG
ACGAGTTCTT
CTGAGCGGGA
CTCTGGGGTT
CGAAATGACC
GACCAAGCGA
CGCCCAACCT
GCCATCACGA
GATTTCGATT
CCACCGCCGC
CTTCTATGAA
AGGTTGGGCT
TCGGAATCGT
TTTCCGGGAC
GCCGGCTGGA
TGATCCTCCA
GCGCGGGGAT
CTCATGCTGG
AGTTCTTCGC
CCACCCTAGG
GGGAGGCTAA
CTGAAACACG
GAAGGAGACA
ATACCGGAAG
GAACCCGCGC
TATGACGGCA
ATAAAAAGAC
AGAATAAAAC
GCACGGTGTT
GGGTCGTTTG
TTCATAAACG
CGGGGTTCGG
TCCCAGGGCT
GGCACTCTGT
CGATACCCCA
CCGAGACCCC
ATTGGGGCCA
ATACGCCCGC
GTTTCTTCCT
TTTCCCCACC
CCACCCCCCA
AGTTCGGGTG
AAGGCCCAGG
GCTCGCAGCC
AACGTCGGGG
CGGCAGGCCC
TGCCATAGCC
TCAGGTTACT
CATATATACT
TTAGATTGAT
TTAAAACTTC
ATTTTTAATT
TAAAAGGATC
TAGGTGAAGA
TCCTTTTTGA
TAATCTCATG
ACCAAAATCC
CTTAACGTGA
GTTTTCGTTC
CACTGAGCGT
CAGACCCCGT
AGAAAAGATC
AAAGGATCTT
CTTGAGATCC
TTTTTTTCTG
CGCGTAATCT
GCTGCTTGCA
AACAAAAAAA
CCACCGCTAC
CAGCGGTGGT
TTGTTTGCCG
GATCAAGAGC
TACCAACTCT
TTTTCCGAAG
GTAACTGGCT
TCAGCAGAGC
GCAGATACCA
AATACTGTCC
TTCTAGTGTA
GCCGTAGTTA
GGCCACCACT
TCAAGAACTC
TGTAGCACCG
CCTACATACC
TCGCTCTGCT
AATCCTGTTA
CCAGTGGCTG
CTGCCAGTGG
CGATAAGTCG
TGTCTTACCG
GGTTGGACTC
AAGACGATAG
TTACCGGATA
AGGCGCAGCG
GTCGGGCTGA
ACGGGGGGTT
CGTGCACACA
GCCCAGCTTG
GAGCGAACGA
CCTACACCGA
ACTGAGATAC
CTACAGCGTG
AGCTATGAGA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCAT
GCAT