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pDsRed1-N1-SATB1(human)

产品货号:P45225

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pDsRed1-N1-SATB1(human)
别称: NM_001131010.4
基因长: 2292bp
质粒宿主: 哺乳细胞
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Kan
筛选标记: Neo/G418
荧光标记: 红色
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
反向引物: Sv40-polyA-R:GAAATTTGTGATGCTATTGC
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

TAGTTATTAA

TAGTAATCAA

TTACGGGGTC

ATTAGTTCAT

AGCCCATATA

TGGAGTTCCG

61

CGTTACATAA

CTTACGGTAA

ATGGCCCGCC

TGGCTGACCG

CCCAACGACC

CCCGCCCATT

121

GACGTCAATA

ATGACGTATG

TTCCCATAGT

AACGCCAATA

GGGACTTTCC

ATTGACGTCA

181

ATGGGTGGAG

TATTTACGGT

AAACTGCCCA

CTTGGCAGTA

CATCAAGTGT

ATCATATGCC

241

AAGTACGCCC

CCTATTGACG

TCAATGACGG

TAAATGGCCC

GCCTGGCATT

ATGCCCAGTA

301

CATGACCTTA

TGGGACTTTC

CTACTTGGCA

GTACATCTAC

GTATTAGTCA

TCGCTATTAC

361

CATGGTGATG

CGGTTTTGGC

AGTACATCAA

TGGGCGTGGA

TAGCGGTTTG

ACTCACGGGG

421

ATTTCCAAGT

CTCCACCCCA

TTGACGTCAA

TGGGAGTTTG

TTTTGGCACC

AAAATCAACG

481

GGACTTTCCA

AAATGTCGTA

ACAACTCCGC

CCCATTGACG

CAAATGGGCG

GTAGGCGTGT

541

ACGGTGGGAG

GTCTATATAA

GCAGAGCTGG

TTTAGTGAAC

CGTCAGATCC

GCTAGCGCTA

601

CCGGACTCAG

ATCTCGAGCT

CAAGCTTCGA

ATTCGCCACC

ATGGATCATT

TGAACGAGGC

661

AACTCAGGGG

AAAGAACATT

CAGAAATGTC

TAACAATGTG

AGTGATCCGA

AGGGTCCACC

721

AGCCAAGATT

GCCCGCCTGG

AGCAGAACGG

GAGCCCGCTA

GGAAGAGGAA

GGCTTGGGAG

781

TACAGGTGCA

AAAATGCAGG

GAGTGCCTTT

AAAACACTCG

GGCCATCTGA

TGAAAACCAA

841

CCTTAGGAAA

GGAACCATGC

TGCCAGTTTT

CTGTGTGGTG

GAACATTATG

AAAACGCCAT

901

TGAATATGAT

TGCAAGGAGG

AGCATGCAGA

ATTTGTGCTG

GTGAGAAAGG

ATATGCTTTT

961

CAACCAGCTG

ATCGAAATGG

CATTGCTGTC

TCTAGGTTAT

TCACATAGCT

CTGCTGCCCA

1021

GGCCAAAGGG

CTAATCCAGG

TTGGAAAGTG

GAATCCAGTT

CCACTGTCTT

ACGTGACAGA

1081

TGCCCCTGAT

GCTACAGTAG

CAGATATGCT

TCAAGATGTG

TATCATGTGG

TCACATTGAA

1141

AATTCAGTTA

CACAGTTGCC

CCAAACTAGA

AGACTTGCCT

CCCGAACAAT

GGTCGCACAC

1201

CACAGTGAGG

AATGCTCTGA

AGGACTTACT

GAAAGATATG

AATCAGAGTT

CATTGGCCAA

1261

GGAGTGCCCC

CTTTCACAGA

GTATGATTTC

TTCCATTGTG

AACAGTACTT

ACTATGCAAA

1321

TGTCTCAGCA

GCAAAATGTC

AAGAATTTGG

AAGGTGGTAC

AAACATTTCA

AGAAGACAAA

1381

AGATATGATG

GTTGAAATGG

ATAGTCTTTC

TGAGCTATCC

CAGCAAGGCG

CCAATCATGT

1441

CAATTTTGGC

CAGCAACCAG

TTCCAGGGAA

CACAGCCGAG

CAGCCTCCAT

CCCCTGCGCA

1501

GCTCTCCCAT

GGCAGCCAGC

CCTCTGTCCG

GACACCTCTT

CCAAACCTGC

ACCCTGGGCT

1561

CGTATCAACA

CCTATCAGTC

CTCAATTGGT

CAACCAGCAG

CTGGTGATGG

CTCAGCTGCT

1621

GAACCAGCAG

TATGCAGTGA

ATAGACTTTT

AGCCCAGCAG

TCCTTAAACC

AACAATACTT

1681

GAACCACCCT

CCCCCTGTCA

GTAGATCTAT

GAATAAGCCT

TTGGAGCAAC

AGGTTTCGAC

1741

CAACACAGAG

GTGTCTTCCG

AAATCTACCA

GTGGGTACGC

GATGAACTGA

AACGAGCAGG

1801

AATCTCCCAG

GCGGTATTTG

CACGTGTGGC

TTTTAACAGA

ACTCAGGGCT

TGCTTTCAGA

1861

AATCCTCCGA

AAGGAAGAGG

ACCCCAAGAC

TGCATCCCAG

TCTTTGCTGG

TAAACCTTCG

1921

GGCTATGCAG

AATTTCTTGC

AGTTACCGGA

AGCTGAAAGA

GACCGAATAT

ACCAGGACGA

1981

AAGGGAAAGG

AGCTTGAATG

CTGCCTCGGC

CATGGGTCCT

GCCCCCCTCA

TCAGCACACC

2041

ACCCAGCCGT

CCTCCCCAGG

TGAAAACAGC

TACTATTGCC

ACTGAAAGGA

ATGGGAAACC

2101

AGAGAACAAT

ACCATGAACA

TTAATGCTTC

CATTTATGAT

GAGATTCAGC

AGGAAATGAA

2161

GCGTGCTAAA

GTGTCTCAAG

CACTGTTTGC

AAAGGTTGCA

GCAACCAAAA

GCCAGGGATG

2221

GTTGTGCGAG

CTGTTACGCT

GGAAAGAAGA

TCCTTCTCCA

GAAAACAGAA

CCCTGTGGGA

2281

GAACCTCTCC

ATGATCCGAA

GGTTCCTCAG

TCTTCCTCAG

CCAGAACGTG

ATGCCATTTA

2341

TGAACAGGAG

AGCAACGCGG

TGCATCACCA

TGGCGACAGG

CCGCCCCACA

TTATCCATGT

2401

TCCAGCAGAG

CAGATTCAGA

GCCCCTCTCC

CACCACCCTT

GGGAAAGGAG

AGTCTAGAGG

2461

CGTTTTCTTA

CCAGGCCTGC

CGACCCCTGC

ACCATGGCTC

GGTGCTGCTC

CTCAGCAACA

2521

GCAGCAGCAA

CAGCAACAGC

AGCAGCAGCA

GCAGCAGGCA

CCGCCGCCTC

CACAGCCACA

2581

GCAGCAGCCA

CAGACAGGCC

CTCGGCTCCC

CCCACGGCAA

CCCACGGTGG

CCTCTCCAGC

2641

AGAGTCAGAT

GAGGAAAACC

GACAGAAGAC

CCGGCCACGA

ACAAAAATTT

CAGTGGAAGC

2701

CTTGGGAATC

CTCCAGAGTT

TCATACAAGA

CGTGGGCCTG

TACCCTGACG

AAGAGGCCAT

2761

CCAGACTCTG

TCTGCCCAGC

TCGACCTTCC

CAAGTACACC

ATCATCAAGT

TCTTTCAGAA

2821

CCAGCGGTAC

TATCTCAAGC

ACCACGGCAA

ACTGAAGGAC

AATTCCGGTT

TAGAGGTCGA

2881

TGTGGCAGAA

TATAAAGAAG

AGGAGCTGCT

GAAGGATTTG

GAAGAGAGTG

TCCAAGATAA

2941

AAATACTAAC

ACCCTTTTTT

CAGTGAAACT

AGAAGAAGAG

CTGTCAGTGG

AAGGAAACAC

3001

AGACATTAAT

ACTGATTTGA

AAGACGGCGG

CGGAGGGAGT

ATGGTGCGCT

CCTCCAAGAA

3061

CGTCATCAAG

GAGTTCATGC

GCTTCAAGGT

GCGCATGGAG

GGCACCGTGA

ACGGCCACGA

3121

GTTCGAGATC

GAGGGCGAGG

GCGAGGGCCG

CCCCTACGAG

GGCCACAACA

CCGTGAAGCT

3181

GAAGGTGACC

AAGGGCGGCC

CCCTGCCCTT

CGCCTGGGAC

ATCCTGTCCC

CCCAGTTCCA

3241

GTACGGCTCC

AAGGTGTACG

TGAAGCACCC

CGCCGACATC

CCCGACTACA

AGAAGCTGTC

3301

CTTCCCCGAG

GGCTTCAAGT

GGGAGCGCGT

GATGAACTTC

GAGGACGGCG

GCGTGGTGAC

3361

CGTGACCCAG

GACTCCTCCC

TGCAGGACGG

CTGCTTCATC

TACAAGGTGA

AGTTCATCGG

3421

CGTGAACTTC

CCCTCCGACG

GCCCCGTAAT

GCAGAAGAAG

ACCATGGGCT

GGGAGGCCTC

3481

CACCGAGCGC

CTGTACCCCC

GCGACGGCGT

GCTGAAGGGC

GAGATCCACA

AGGCCCTGAA

3541

GCTGAAGGAC

GGCGGCCACT

ACCTGGTGGA

GTTCAAGTCC

ATCTACATGG

CCAAGAAGCC

3601

CGTGCAGCTG

CCCGGCTACT

ACTACGTGGA

CTCCAAGCTG

GACATCACCT

CCCACAACGA

3661

GGACTACACC

ATCGTGGAGC

AGTACGAGCG

CACCGAGGGC

CGCCACCACC

TGTTCCTGTA

3721

GCGGCCGCGA

CTCTAGATCA

TAATCAGCCA

TACCACATTT

GTAGAGGTTT

TACTTGCTTT

3781

AAAAAACCTC

CCACACCTCC

CCCTGAACCT

GAAACATAAA

ATGAATGCAA

TTGTTGTTGT

3841

TAACTTGTTT

ATTGCAGCTT

ATAATGGTTA

CAAATAAAGC

AATAGCATCA

CAAATTTCAC

3901

AAATAAAGCA

TTTTTTTCAC

TGCATTCTAG

TTGTGGTTTG

TCCAAACTCA

TCAATGTATC

3961

TTAAGGCGTA

AATTGTAAGC

GTTAATATTT

TGTTAAAATT

CGCGTTAAAT

TTTTGTTAAA

4021

TCAGCTCATT

TTTTAACCAA

TAGGCCGAAA

TCGGCAAAAT

CCCTTATAAA

TCAAAAGAAT

4081

AGACCGAGAT

AGGGTTGAGT

GTTGTTCCAG

TTTGGAACAA

GAGTCCACTA

TTAAAGAACG

4141

TGGACTCCAA

CGTCAAAGGG

CGAAAAACCG

TCTATCAGGG

CGATGGCCCA

CTACGTGAAC

4201

CATCACCCTA

ATCAAGTTTT

TTGGGGTCGA

GGTGCCGTAA

AGCACTAAAT

CGGAACCCTA

4261

AAGGGAGCCC

CCGATTTAGA

GCTTGACGGG

GAAAGCCGGC

GAACGTGGCG

AGAAAGGAAG

4321

GGAAGAAAGC

GAAAGGAGCG

GGCGCTAGGG

CGCTGGCAAG

TGTAGCGGTC

ACGCTGCGCG

4381

TAACCACCAC

ACCCGCCGCG

CTTAATGCGC

CGCTACAGGG

CGCGTCAGGT

GGCACTTTTC

4441

GGGGAAATGT

GCGCGGAACC

CCTATTTGTT

TATTTTTCTA

AATACATTCA

AATATGTATC

4501

CGCTCATGAG

ACAATAACCC

TGATAAATGC

TTCAATAATA

TTGAAAAAGG

AAGAGTCCTG

4561

AGGCGGAAAG

AACCAGCTGT

GGAATGTGTG

TCAGTTAGGG

TGTGGAAAGT

CCCCAGGCTC

4621

CCCAGCAGGC

AGAAGTATGC

AAAGCATGCA

TCTCAATTAG

TCAGCAACCA

GGTGTGGAAA

4681

GTCCCCAGGC

TCCCCAGCAG

GCAGAAGTAT

GCAAAGCATG

CATCTCAATT

AGTCAGCAAC

4741

CATAGTCCCG

CCCCTAACTC

CGCCCATCCC

GCCCCTAACT

CCGCCCAGTT

CCGCCCATTC

4801

TCCGCCCCAT

GGCTGACTAA

TTTTTTTTAT

TTATGCAGAG

GCCGAGGCCG

CCTCGGCCTC

4861

TGAGCTATTC

CAGAAGTAGT

GAGGAGGCTT

TTTTGGAGGC

CTAGGCTTTT

GCAAAGATCG

4921

ATCAAGAGAC

AGGATGAGGA

TCGTTTCGCA

TGATTGAACA

AGATGGATTG

CACGCAGGTT

4981

CTCCGGCCGC

TTGGGTGGAG

AGGCTATTCG

GCTATGACTG

GGCACAACAG

ACAATCGGCT

5041

GCTCTGATGC

CGCCGTGTTC

CGGCTGTCAG

CGCAGGGGCG

CCCGGTTCTT

TTTGTCAAGA

5101

CCGACCTGTC

CGGTGCCCTG

AATGAACTGC

AAGACGAGGC

AGCGCGGCTA

TCGTGGCTGG

5161

CCACGACGGG

CGTTCCTTGC

GCAGCTGTGC

TCGACGTTGT

CACTGAAGCG

GGAAGGGACT

5221

GGCTGCTATT

GGGCGAAGTG

CCGGGGCAGG

ATCTCCTGTC

ATCTCACCTT

GCTCCTGCCG

5281

AGAAAGTATC

CATCATGGCT

GATGCAATGC

GGCGGCTGCA

TACGCTTGAT

CCGGCTACCT

5341

GCCCATTCGA

CCACCAAGCG

AAACATCGCA

TCGAGCGAGC

ACGTACTCGG

ATGGAAGCCG

5401

GTCTTGTCGA

TCAGGATGAT

CTGGACGAAG

AGCATCAGGG

GCTCGCGCCA

GCCGAACTGT

5461

TCGCCAGGCT

CAAGGCGAGC

ATGCCCGACG

GCGAGGATCT

CGTCGTGACC

CATGGCGATG

5521

CCTGCTTGCC

GAATATCATG

GTGGAAAATG

GCCGCTTTTC

TGGATTCATC

GACTGTGGCC

5581

GGCTGGGTGT

GGCGGACCGC

TATCAGGACA

TAGCGTTGGC

TACCCGTGAT

ATTGCTGAAG

5641

AGCTTGGCGG

CGAATGGGCT

GACCGCTTCC

TCGTGCTTTA

CGGTATCGCC

GCTCCCGATT

5701

CGCAGCGCAT

CGCCTTCTAT

CGCCTTCTTG

ACGAGTTCTT

CTGAGCGGGA

CTCTGGGGTT

5761

CGAAATGACC

GACCAAGCGA

CGCCCAACCT

GCCATCACGA

GATTTCGATT

CCACCGCCGC

5821

CTTCTATGAA

AGGTTGGGCT

TCGGAATCGT

TTTCCGGGAC

GCCGGCTGGA

TGATCCTCCA

5881

GCGCGGGGAT

CTCATGCTGG

AGTTCTTCGC

CCACCCTAGG

GGGAGGCTAA

CTGAAACACG

5941

GAAGGAGACA

ATACCGGAAG

GAACCCGCGC

TATGACGGCA

ATAAAAAGAC

AGAATAAAAC

6001

GCACGGTGTT

GGGTCGTTTG

TTCATAAACG

CGGGGTTCGG

TCCCAGGGCT

GGCACTCTGT

6061

CGATACCCCA

CCGAGACCCC

ATTGGGGCCA

ATACGCCCGC

GTTTCTTCCT

TTTCCCCACC

6121

CCACCCCCCA

AGTTCGGGTG

AAGGCCCAGG

GCTCGCAGCC

AACGTCGGGG

CGGCAGGCCC

6181

TGCCATAGCC

TCAGGTTACT

CATATATACT

TTAGATTGAT

TTAAAACTTC

ATTTTTAATT

6241

TAAAAGGATC

TAGGTGAAGA

TCCTTTTTGA

TAATCTCATG

ACCAAAATCC

CTTAACGTGA

6301

GTTTTCGTTC

CACTGAGCGT

CAGACCCCGT

AGAAAAGATC

AAAGGATCTT

CTTGAGATCC

6361

TTTTTTTCTG

CGCGTAATCT

GCTGCTTGCA

AACAAAAAAA

CCACCGCTAC

CAGCGGTGGT

6421

TTGTTTGCCG

GATCAAGAGC

TACCAACTCT

TTTTCCGAAG

GTAACTGGCT

TCAGCAGAGC

6481

GCAGATACCA

AATACTGTCC

TTCTAGTGTA

GCCGTAGTTA

GGCCACCACT

TCAAGAACTC

6541

TGTAGCACCG

CCTACATACC

TCGCTCTGCT

AATCCTGTTA

CCAGTGGCTG

CTGCCAGTGG

6601

CGATAAGTCG

TGTCTTACCG

GGTTGGACTC

AAGACGATAG

TTACCGGATA

AGGCGCAGCG

6661

GTCGGGCTGA

ACGGGGGGTT

CGTGCACACA

GCCCAGCTTG

GAGCGAACGA

CCTACACCGA

6721

ACTGAGATAC

CTACAGCGTG

AGCTATGAGA

AAGCGCCACG

CTTCCCGAAG

GGAGAAAGGC

6781

GGACAGGTAT

CCGGTAAGCG

GCAGGGTCGG

AACAGGAGAG

CGCACGAGGG

AGCTTCCAGG

6841

GGGAAACGCC

TGGTATCTTT

ATAGTCCTGT

CGGGTTTCGC

CACCTCTGAC

TTGAGCGTCG

6901

ATTTTTGTGA

TGCTCGTCAG

GGGGGCGGAG

CCTATGGAAA

AACGCCAGCA

ACGCGGCCTT

6961

TTTACGGTTC

CTGGCCTTTT

GCTGGCCTTT

TGCTCACATG

TTCTTTCCTG

CGTTATCCCC

7021

TGATTCTGTG

GATAACCGTA

TTACCGCCAT

GCAT

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