名称: | pFC902 |
---|---|
别称: | Plasmid#106904 |
基因长: | |
质粒宿主: | 丝状真菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Amp |
筛选标记: | pyrG |
荧光标记: | |
启动子: | U3 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GCGCTGCGAT
GATACCGCGA
GAACCACGCT
CACCGGCTCC
GGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
CGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCGCTACAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
TTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTCA
GTGTTACAAC
CAATTAACCA
ATTCTGAACA
TTATCGCGAG
CCCATTTATA
CCTGAATATG
GCTCATAACA
CCCCTTGTTT
GCCTGGCGGC
AGTAGCGCGG
TGGTCCCACC
TGACCCCATG
CCGAACTCAG
AAGTGAAACG
CCGTAGCGCC
GATGGTAGTG
TGGGGACTCC
CCATGCGAGA
GTAGGGAACT
GCCAGGCATC
AAATAAAACG
AAAGGCTCAG
TCGAAAGACT
GGGCCTTTCG
CCCGGGCTAA
TTATGGGGTG
TCGCCCTTAT
TCGACTCTAT
AGTGAAGTTC
CTATTCTCTA
GAAAGTATAG
GAACTTCTGA
AGTGGGGATT
TAAATGCGGC
CGCGCTGAGG
GTTTAATCGA
CGAAGCAGCT
GACGGCCAGT
GCCAAGCTTA
ACGCGTACCG
GGCCCAGTAT
ATGTTCCGCA
GATGACTGGA
GCTCTGCCAT
ACGTGCCCTC
TCAAGCACCA
TTTGTTCCAT
CTACAGAGAC
TAGTCACCAA
CTAGTCTATC
AAGACTCACA
GGGTACATTG
CTGAGACCAA
CTGACCAGAG
GCAGGGTAGC
GGATTGACGG
CTCCATCTCC
TTCACTTACA
AGGTCTATTG
AAAGCCCTTT
AGCATCACCA
AGCGGAGAAT
AGATTGTTAA
GCTTATTTTT
TGTATACTGT
TTTGTGATAG
CACGAAGTTT
TTCCACGGTA
TCTTGTTAAA
AATATATATT
TGTGGCGGGC
TTACCTACAT
CAAATTAATA
AGAGACTAAT
TATAAACTAA
ACACACAAGC
AAGCTACTTT
AGGGTAAAAG
TTTATAAATG
CTTTTGACGT
ATAAACGTTG
CTTGTATTTA
TTATTACAAT
TAAAGGTGGA
TAGAAAACCT
AGAGACTAGT
TAGAAACTAA
TCTCAGGTTT
GCGTTAAACT
AAATCAGAGC
CCGAGAGGTT
AACAGAACCT
AGAAGGGGAC
TAGATATCCG
GGTAGGGAAA
CAAAAAAAAA
AACAAGACAG
CCACATATTA
GGGAGACTAG
TTAGAAGCTA
GTTCCAGGAC
TAGGAAAATA
AAAGACAATG
ATACCACAGT
CTAGTTGACA
ACTAGATAGA
TTCTAGATTG
AGGCCAAAGT
CTCTGAGATC
CAGGTTAGTT
GCAACTAATA
CTAGTTAGTA
TCTAGTCTCC
TATAACTCTG
AAGCTAGAAT
AACTTACTAC
TATTATCCTC
ACCACTGTTC
AGCTGCGCAA
ACGGAGTGAT
TGCAAGGTGT
TCAGAGACTA
GTTATTGACT
AGTCAGTGAC
TAGCAATAAC
TAACAAGGTA
TTAACCTACC
ATGTCTGCCA
TCACCCTGCA
CTTCCTCGGG
CTCAGCAGCC
TTTTCCTCCT
CATTTTCATG
CTCATTTTCC
TTGTTTAAGA
CTGTGACTAG
TCGAAGACTA
GTCCAGAACC
ACAAAGGAGA
AATGTCTTAC
CACTTTCTTC
ATTGCTTGTC
TCTTTTGCAT
TATCCATGTC
TGCAACTAGT
TAGAGTCTAG
TTAGTGACTA
GTCCGACGAG
GACTTGCTTG
TCTCCGGATT
GTTGGAGGAA
CTCTCCAGGG
CCTCAAGATC
CACAACAGAG
CCTTCTAGAA
GACTGGTCAA
TAACTAGTTG
GTCTTTGTCT
GAGTCTGACT
TACGAGGTTG
CATACTCGCT
CCCTTTGCCT
CGTCAATCGA
TGAGAAAAAG
CGCCAAAACT
CGCAATATGG
CTTTGAACCA
CACGGTGCTG
AGACTAGTTA
GAATCTAGTC
CCAAACTAGC
TTGGATAGCT
TACCTTTGCC
CTTTGCGTTG
CGACAGGTCT
TGCAGGGTAT
GGTTCCTTTC
TCACCAGCTG
ATTTAGCTGC
CTTGCTACCC
TCACGGCGGA
TCTGCATAAA
GAGTGGCTAG
AGGTTATAAA
TTAGCACTGA
TCCTAGGTAC
GGGGCTGAAT
GTAACTTGCC
CTTCCTTTCT
CATCGCGCGG
CAAGACAGGC
TTGCTCAAAT
TCCTACCAGT
CACAGGGGTA
TGCACGGCGT
ACGGACCACT
TGAACTAGTC
ACAGATTAGT
TAGCAACTAG
TCTGCATTGA
ATGGCTGTAC
TTACGGGCCC
TCGCCATTGT
CCTGATCATT
TCCAGCTTCA
CCCTCGTTGC
TGCAAAGTAG
TTAGTGACTA
GTCAAGGACT
AGTTGAAATG
GGAGAAGAAA
CTCACGAATT
CTCGACACCC
TTAGTATTGT
GGTCCTTGGA
CTTGGTGCTG
CTATATATTA
GCTAATACAC
TAGTTAGACT
CACGGAAACT
TACGCAGCTC
GCTTGCGCTT
CTTGGTAGGA
GTCGGGGTTG
GGAGAACAGT
GCCTTCAAAC
AAGCCTTCAT
ACCATGCTAC
TTGACTAGTC
AGGGACTAGT
CACCAAGTAA
TCTAGATAGG
ACTTGCCTTT
GGCCTCCATC
AGTTCCTTCA
TAGTGGGAGG
TCCATTGTGC
AATGTAAACT
CCATGCCGTG
GGAGTTCTTG
TCCTTCAAGT
GCTTGACCAA
TATGTTTCTG
TTGGCAGAGG
GAACCTGTCA
ACTAGTTAAT
AACTAGTCAG
AAACTAGTAT
AGCAGTAGAC
TCACTGTACG
CTTGAGGCAT
CCCTTCACTC
GGCAGTAGAC
TTCATATGGA
TGGATATCAG
GCACGCCATT
GTCGTCCTGT
GGACTAGTCA
GTAACTAGGC
TTAAAGCTAG
TCGGGTCGGC
TTACTATCTT
GAAATCCGGC
AGCGTAAGCT
CCCCGTCCTT
AACTGCCTCG
AGATAGTGAC
AGTACTCTGG
GGACTTTCGG
AGATCGTTAT
CGCGAATGCT
CGGCATACTA
ATCGTTGACT
AGTCTTGGAC
TAGTCCCGAG
CAAAAAGGAT
TGGAGGAGGA
GGAGGAAGGT
GAGAGTGAGA
CAAAGAGCGA
AATAAGAGCT
TCAAAGGCTA
TCTCTAAGCA
GTATGAAGGT
TAAGTATCTA
GTTCTTGACT
AGATTTAAAA
GAGATTTCGA
CTAGTTATGT
ACCTGGAGTT
TGGATATAGG
AATGTGTTGT
GGTAACGAAA
TGTAAGGGGG
AGGAAAGAAA
AAGTCGGTCA
AGAGGTAACT
CTAAGTCGGC
CATTCCTTTT
TGGGAGGCGC
TAACCATAAA
CGGCATGGTC
GACTTAGAGT
TAGCTCAGGG
AATTTAGGGA
GTTATCTGCG
ACCACCGAGG
AACGGCGGAA
TGCCAAAGAA
TCCCGATGGA
GCTCTAGCTG
GCGGTTGACA
ACCCCACCTT
TTGGCGTTTC
TGCGGCGTTG
CAGGCGGGAC
TGGATACTTC
GTAGAACCAG
AAAGGCAAGG
CAGAACGCGC
TCAGCAAGAG
TGTTGGAAGT
GATAGCATGA
TGTGCCTTGT
TAACTAGGTC
AAAATCTGCA
GTATGCTTGA
TGTTATCCAA
AGTGTGAGAG
AGGAAGGTCC
AAACATACAC
GATTGGGAGA
GGGCCTAGGT
ATAAGAGTTT
TTGAGTAGAA
CGCATGTGAG
CCCAGCCATC
TCGAGGAGAT
TAAACACGGG
CCGGCATTTG
ATGGCTATGT
TAGTACCCCA
ATGGAAACGG
TGAGAGTCCA
GTGGTCGCAG
ATAACTCCCT
AAATTCCCTG
AGCTAACTCT
AAGTCGACCA
TGCCGTTTAT
GGTTAGCGCC
TCCCAAAAAG
GAATGGCCGA
CTTAGAGTTA
CCTCTTGACC
GACTTTTTCT
TTCCTCCCCC
TTACATTTCG
TTACCACAAC
ACATTCCTAT
ATCCAAACTC
CAGGTACATA
ACTAGTCGAA
ATCTCTTTTA
AATCTAGTCA
AGAACTAGAT
ACTTAACCTT
CATACTGCTT
AGAGATAGCC
TTTGAAGCTC
TTATTTCGCT
CTTTGTCTCA
CTCTCACCTT
CCTCCTCCTC
CTCCAATCCT
TTTTGCTCGG
GACTAGTCCA
AGACTAGTCA
ACGATTAGTA
TGCCGAGCAT
TCGCGATAAC
GATCTCCGAA
AGTCCCCAGA
GTACTGTCAC
TATCTCGAGG
CAGTTAAGGA
CGGGGAGCTT
ACGCTGCCGG
ATTTCAAGAT
AGTAAGCCGA
CCCGACTAGC
TTTAAGCCTA
GTTACTGACT
AGTCCACAGG
ACGACAATGG
CGTGCCTGAT
ATCCATCCAT
ATGAAGTCTA
CTGCCGAGTG
AAGGGATGCC
TCAAGCGTAC
AGTGAGTCTA
CTGCTATACT
AGTTTCTGAC
TAGTTATTAA
CTAGTTGACA
GGTTCCCTCT
GCCAACAGAA
ACATATTGGT
CAAGCACTTG
AAGGACAAGA
ACTCCCACGG
CATGGAGTTT
ACATTGCACA
ATGGACCTCC
CACTATGAAG
GAACTGATGG
AGGCCAAAGG
CAAGTCCTAT
CTAGATTACT
TGGTGACTAG
TCCCTGACTA
GTCAAGTAGC
ATGGTATGAA
GGCTTGTTTG
AAGGCACTGT
TCTCCCAACC
CCGACTCCTA
CCAAGAAGCG
CAAGCGAGCT
GCGTAAGTTT
CTGTGAGTCT
AACTAGTGTA
TTAGCTAATA
TATAGCAGCA
CCAAGTCCAA
GGACCACAAT
ACTAAGGGTG
TCGAGAATTC
GTGAGTTTCT
TCTCCCATTT
CAACTAGTCC
TTGACTAGTC
ACTAACTACT
TTGCAGCAAC
GAGGGTGAAG
CTGGAAATGA
TCAGGACAAT
GGCGAGGGCC
CGTAAGTACA
GCCATTCAAT
GCAGACTAGT
TGCTAACTAA
TCTGTGACTA
GTTCAAGTGG
TCCGTACGCC
GTGCATACCC
CTGTGACTGG
TAGGAATTTG
AGCAAGCCTG
TCTTGCCGCG
CGATGAGAAA
GGAAGGGCAA
GTTACATTCA
GCCCCGTACC
TAGGATCAGT
GCTAATTTAT
AACCTCTAGC
CACTCTTTAT
GCAGATCCGC
CGTGAGGGTA
GCAAGGCAGC
TAAATCAGCT
GGTGAGAAAG
GAACCATACC
CTGCAAGACC
TGTCGCAACG
CAAAGGGCAA
AGGTAAGCTA
TCCAAGCTAG
TTTGGGACTA
GATTCTAACT
AGTCTCAGCA
CCGTGTGGTT
CAAAGCCATA
TTGCGAGTTT
TGGCGCTTTT
TCTCATCGAT
TGACGAGGCA
AAGGGAGCGA
GTATGCAACC
TCGTAAGTCA
GACTCAGACA
AAGACCAACT
AGTTATTGAC
CAGTCTTCTA
GAAGGCTCTG
TTGTGGATCT
TGAGGCCCTG
GAGAGTTCCT
CCAACAATCC
GGAGACAAGC
AAGTCCTCGT
CGGACTAGTC
ACTAACTAGA
CTCTAACTAG
TTGCAGACAT
GGATAATGCA
AAAGAGACAA
GCAATGAAGA
AAGTGGTAAG
ACATTTCTCC
TTTGTGGTTC
TGGACTAGTC
TTCGACTAGT
CACAGTCTTA
AACAAGGAAA
ATGAGCATGA
AAATGAGGAG
GAAAAGGCTG
CTGAGCCCGA
GGAAGTGCAG
GGTGATGGCA
GACATGGTAG
GTTAATACCT
TGTTAGTTAT
TGCTAGTCAC
TGACTAGTCA
ATAACTAGTC
TCTGAACACC
TTGCAATCAC
TCCGTTTGCG
CAGCTGAACA
GTGGTGAGGA
TAATAGTAGT
AAGTTATTCT
AGCTTCAGAG
TTATAGGAGA
CTAGATACTA
ACTAGTATTA
GTTGCAACTA
ACCTGGATCT
CAGAGACTTT
GGCCTCAATC
TAGAATCTAT
CTAGTTGTCA
ACTAGACTGT
GGTATCATTG
TCTTTTATTT
TCCTAGTCCT
GGAACTAGCT
TCTAACTAGT
CTCCCTAATA
TGTGGCTGTC
TTGTTTTTTT
TTTTGTTTCC
CTACCCGGAT
ATCTAGTCCC
CTTCTAGGTT
CTGTTAACCT
CTCGGGCTCT
GATTTAGTTT
AACGCAAACC
TGAGATTAGT
TTCTAACTAG
TCTCTAGGTT
TTCTATCCAC
CTTTAATTGT
AATAATAAAT
ACAAGCAACG
TTTATACGTC
AAAAGCATTT
ATAAACTTTT
ACCCTAAAGT
AGCTTGCTTG
TGTGTTTAGT
TTATAATTAG
TCTCTTATTA
ATTTGATGTA
GGTAAGCCCG
CCACAAATAT
ATATTTTTAA
CAAGATACCG
TGGAAAAACT
TCGTGCTATC
ACAAAACAGT
ATACAAAAAA
TAAGCTTAAC
AATCTATTCT
CCGCTTGGTG
ATGCTAAAGG
GCTTTCAATA
GACCTTGTAA
GTGAAGGAGA
TGGAGCCGTC
AATCCGCTAC
CCTGCCTCTG
GTCAGTTGGT
CTCAGCAATG
TACCCTGTGA
GTCTTGATAG
ACTAGTTGGT
GACTAGTCTC
TGTAGATGGA
ACAAATGGTG
CTTGAGAGGG
CACGTATGGC
AGAGCTCCAG
TCATCTGCGG
AACATATACT
GGGCCCGGGA
AGATCTCATG
GTCATAGCTG
TTTCCGCTGA
GGGTTTAATG
ATCACATAGA
TGCTCGGTTG
ACAGGACCAC
CTATAGCAAG
TACTTTGTAG
GAGGTTCTCC
TAACGCTTGG
CTTCAAATCC
AATCAGGATA
TCAGTGAGTT
ACTGTTTGTC
ATCGCATCCT
CATGAAGTCG
CTCAACAGCC
ACTGAGAAGC
AAATATTTGG
GAGACCATCC
CTGATGTTGA
AATTTTACGC
TGGAGCCCAT
TCACCGGTGA
GCTCGGGGAC
AGTCTGGCCA
GTGGAGCGGA
AAATCTTCAA
CTAACTCTGA
TTGGTACCAC
AGGTAGCCCC
ACAGAACAAA
CCGAGCAAAC
ATGAAAATTT
TCGCTTGAGG
TTAGCGCACT
CGCTAGCGCC
TGCCCGCAAA
TATAAAAGGC
CCCGAAATCC
CATGCATTTT
GGAAATCAAG
CGACTCTACG
TATGTCATCC
CCCTCCCTAC
CTTTGGGAAA
ACCTCTCAAC
TGCAAGTCAG
AACATTTTGC
TAACAGCGCA
TCATTGGTCT
AGTGGTAGAA
TTCATCGTTG
CCATCGATGA
GGCCCGTGTT
CGATTCACGG
ATGATGCAGG
CGGAGTATCA
TAACATCGGT
TTTAGAGCTA
GAAATAGCAA
GTTAAAATAA
GGCTAGTCCG
TTATCAACTT
GAAAAAGTGG
CACCGAGTCG
GTGCTTTTGC
ATCATTGGTC
TAGTGGTAGA
ATTCATCGTT
GCCATCGATG
AGGCCCGTGT
TCGATTCACG
GATGATGCAT
GTCAATTTTT
ACACTTGATG
TTGGTGTAAT
CAGGCGATCA
GAGTTTGCTC
AAATTCTCTC
TTGTTTTAGA
TTCTGAAGAA
TATAATTTTT
ACATGACTAG
TTCATTTTAC
TTCCAAGTAG
ACTATTTGTA
GATTGAGATT
GCCGTCAACT
TTATTCATAG
AATCACATTA
AATTGCTCAT
TGACCTGCAT
GTCTATGCAC
ACATTCACAT
CTATCTTCTC
AGGGTATTAA
GACCTCAGCC
GAGACAGCAG
AATCACCGCC
CAAGTTAAGC
CTTTGTGCTG
ATCATGCTCT
CGAACGGGCC
AAGTTCGGGA
AAAGCAAAGG
AGCGTTTAGT
GAGGGGCAAT
TTGACTCACC
TCCCAGGCAA
CAGATGAGGG
GGGCAAAAAG
AAAGAAATTT
TCGTGAGTCA
ATATGGATTC
CGAGCATCAT
TTTCTTGCGG
TCTATCTTGC
TACGTATGTT
GATCTTGACG
CTGTGGATCA
AGCAACGCCA
CTCGCTCGCT
CCATCGCAGG
CTGGTCGCAG
ACAAATTAAA
AGGCGGCAAA
CTCGTACAGC
CGCGGGGTTG
TCCGCTGCAA
AGTACAGAGT
GATAAAAGCC
GCCATGCGAC
CATCAACGCG
TTGATGCCCA
GCTTTTTCGA
TCCGAGAATC
CACCGTAGAG
GCGATAGCAA
GTAAAGAAAA
GCTAAACAAA
AAAAAATTTC
TGCCCCTAAG
CCATGAAAAC
GAGATGGGGT
GGAGCAGAAC
CAAGGAAAGA
GTCGCGCTGG
GCTGCCGTTC
CGGAAGGTGT
TGTAAAGGCT
CGACGCCCAA
GGTGGGAGTC
TAGGAGAAGA
ATTTGCATCG
GGAGTGGGGC
GGGTTACCCC
TCCATATCCA
ATGACAGATA
TCTACCAGCC
AAGGGTTTGA
GCCCGCCCGC
TTAGTCGTCG
TCCTCGCTTG
CCCCTCCATA
AAAGGATTTC
CCCTCCCCCT
CCCACAAAAT
TTTCTTTCCC
TTCCTCTCCT
TGTCCGCTTC
AGTACGTATA
TCTTCCCTTC
CCTCGCTTCT
CTCCTCCATC
CTTCTTTCAT
CCATCTCCTG
CTAACTTCTC
TGCTCAGCAC
CTCTACGCAT
TACTAGCCGT
AGTATCTGAG
CACTTCTCCC
TTTTATATTC
CACAAAACAT
AACACAACCT
TCACCATGGA
CAAGAAGTAT
AGCATCGGGC
TGGACATTGG
AACGAACTCG
GTTGGTTGGG
CTGTGATTAC
GGACGAATAC
AAGGTGCCAT
CCAAGAAGTT
TAAGGTCCTG
GGAAACACCG
ACCGTCACTC
AATCAAGAAG
AATCTCATTG
GAGCCCTGCT
CTTCGATAGT
GGGGAGACCG
CCGAAGCTAC
TCGACTGAAG
CGAACGGCTC
GCCGGCGTTA
TACACGACGC
AAGAATCGCA
TCTGCTACCT
CCAGGAGATT
TTCAGCAACG
AAATGGCTAA
GGTTGATGAC
TCATTCTTTC
ATCGACTCGA
AGAAAGTTTC
TTGGTCGAGG
AGGATAAGAA
GCACGAGCGC
CATCCGATCT
TTGGTAACAT
TGTGGATGAG
GTTGCCTATC
ACGAAAAGTA
CCCAACTATC
TATCATCTTC
GTAAGAAGCT
GGTCGATAGC
ACGGACAAGG
CTGATTTGCG
ACTTATCTAC
CTGGCACTCG
CGCACATGAT
TAAGTTCCGC
GGCCATTTTC
TTATCGAGGG
TGACCTGAAC
CCCGATAATT
CTGACGTTGA
TAAGCTCTTC
ATCCAGTTGG
TCCAAACCTA
CAATCAGCTG
TTTGAGGAAA
ACCCTATTAA
TGCATCTGGC
GTGGACGCCA
AGGCTATCCT
TTCGGCGCGC
CTGTCTAAGT
CGCGGCGTTT
GGAGAACCTT
ATCGCACAAC
TCCCCGGCGA
AAAGAAGAAC
GGCCTCTTCG
GTAATTTGAT
TGCGTTGTCA
CTTGGTCTGA
CTCCTAACTT
CAAGAGTAAT
TTTGACCTGG
CAGAGGATGC
GAAGCTCCAG
TTGTCTAAGG
ATACGTATGA
TGACGATCTC
GACAACTTGC
TTGCCCAAAT
CGGTGACCAG
TACGCTGATC
TTTTCCTGGC
CGCTAAGAAT
CTCTCAGATG
CAATCCTGCT
CAGTGACATT
TTGCGGGTCA
ACACCGAGAT
TACTAAGGCC
CCCCTGTCAG
CTAGTATGAT
CAAGCGGTAT
GATGAGCACC
ATCAGGACCT
CACCTTGCTT
AAGGCCCTCG
TGCGTCAGCA
ATTGCCTGAG
AAGTATAAGG
AAATCTTCTT
TGACCAATCC
AAGAACGGAT
ACGCAGGGTA
TATTGATGGC
GGTGCGAGCC
AGGAGGAATT
CTACAAGTTT
ATCAAGCCGA
TTTTGGAGAA
GATGGACGGC
ACTGAGGAAC
TGCTCGTCAA
GCTGAATCGC
GAAGATTTGC
TTCGTAAGCA
ACGAACGTTC
GACAACGGCT
CCATCCCGCA
CCAGATTCAT
CTGGGCGAGC
TCCACGCCAT
CCTTCGACGC
CAGGAAGATT
TCTACCCATT
TCTGAAGGAC
AACCGTGAGA
AGATCGAAAA
GATTCTTACA
TTCCGAATCC
CCTACTATGT
GGGACCTTTG
GCCCGTGGGA
ATTCCCGATT
TGCTTGGATG
ACCCGAAAGA
GCGAGGAAAC
CATCACTCCG
TGGAACTTCG
AGGAAGTCGT
GGACAAGGGT
GCATCCGCGC
AGAGCTTCAT
TGAGCGGATG
ACCAATTTTG
ATAAGAACCT
TCCGAATGAA
AAGGTCCTGC
CAAAGCATTC
GCTGCTCTAC
GAGTATTTCA
CCGTGTATAA
CGAACTGACT
AAGGTCAAGT
ACGTGACGGA
GGGAATGCGG
AAGCCAGCCT
TCCTCTCAGG
GGAACAAAAG
AAGGCTATCG
TCGATTTGCT
TTTTAAGACC
AATCGTAAAG
TGACTGTTAA
GCAGCTGAAG
GAGGATTATT
TCAAGAAGAT
TGAATGTTTC
GACTCCGTCG
AGATCAGCGG
CGTGGAAGAT
CGCTTTAACG
CTTCCCTCGG
TACCTACCAC
GACCTGCTCA
AGATCATTAA
GGACAAGGAT
TTCCTCGATA
ACGAGGAAAA
TGAGGACATC
TTGGAAGATA
TTGTCCTCAC
GTTGACACTT
TTTGAGGACC
GCGAAATGAT
CGAGGAACGG
CTCAAGACAT
ATGCCCATTT
GTTCGACGAT
AAGGTGATGA
AGCAGCTGAA
GCGGCGTCGA
TACACCGGAT
GGGGTCGCCT
TAGCCGGAAG
CTGATCAACG
GCATTCGAGA
TAAGCAATCT
GGTAAGACTA
TCTTGGATTT
CCTTAAGTCG
GACGGCTTCG
CCAACCGCAA
TTTTATGCAG
CTTATTCACG
ACGATTCCCT
GACGTTCAAG
GAGGACATCC
AGAAGGCACA
AGTCTCAGGA
CAAGGGGATT
CCCTGCACGA
GCATATCGCC
AACCTGGCTG
GATCCCCGGC
GATCAAGAAG
GGGATTCTTC
AGACCGTCAA
GGTTGTCGAC
GAGCTGGTCA
AGGTGATGGG
CCGTCATAAG
CCAGAAAACA
TCGTGATTGA
GATGGCCCGA
GAAAATCAGA
CCACTCAAAA
GGGTCAGAAG
AACAGCCGCG
AGCGGATGAA
GCGGATCGAG
GAAGGCATTA
AGGAACTTGG
TTCTCAGATC
CTGAAGGAGC
ACCCTGTTGA
AAACACACAG
CTCCAAAATG
AGAAGCTGTA
TCTCTACTAT
TTGCAAAATG
GACGCGACAT
GTACGTCGAT
CAGGAGCTCG
ACATTAACCG
GTTGTCGGAC
TACGATGTTG
ACCATATCGT
CCCGCAATCC
TTCCTTAAGG
ACGATAGCAT
TGATAACAAG
GTGCTGACTC
GCTCAGATAA
GAACCGGGGC
AAGTCCGACA
ATGTTCCAAG
CGAGGAAGTG
GTTAAGAAGA
TGAAGAACTA
CTGGCGCCAA
TTGCTTAATG
CCAAGCTCAT
CACACAGCGC
AAGTTTGACA
ACTTGACCAA
GGCCGAGCGG
GGAGGGCTGA
GTGAACTCGA
TAAGGCTGGC
TTCATCAAGC
GTCAACTCGT
GGAGACGCGA
CAGATCACAA
AGCACGTTGC
TCAGATTCTG
GACTCCCGGA
TGAACACAAA
GTACGACGAG
AATGATAAGC
TCATCCGTGA
AGTTAAGGTC
ATTACCCTCA
AGTCTAAGTT
GGTGTCGGAT
TTCCGCAAGG
ACTTCCAATT
TTATAAGGTT
CGGGAGATCA
ACAATTATCA
CCATGCACAT
GATGCGTACC
TCAACGCAGT
CGTGGGAACT
GCGCTCATCA
AGAAGTATCC
CAAGTTGGAG
TCCGAATTCG
TCTACGGGGA
TTATAAGGTT
TACGACGTCC
GCAAGATGAT
CGCCAAGAGT
GAGCAGGAAA
TTGGCAAGGC
CACGGCTAAG
TATTTCTTTT
ACTCCAACAT
CATGAATTTC
TTTAAGACGG
AGATCACACT
CGCCAATGGA
GAAATCCGTA
AGCGACCTTT
GATTGAGACC
AACGGCGAGA
CTGGTGAAAT
CGTTTGGGAT
AAGGGGCGCG
ACTTCGCTAC
CGTGCGGAAG
GTTCTGAGCA
TGCCGCAAGT
CAATATCGTC
AAGAAAACCG
AGGTGCAGAC
AGGCGGTTTC
TCTAAGGAAT
CGATTCTTCC
AAAGCGTAAC
TCTGACAAGC
TGATCGCTCG
AAAGAAGGAT
TGGGACCCCA
AGAAGTATGG
AGGGTTCGAT
TCTCCTACAG
TGGCATACTC
GGTTCTCGTT
GTCGCGAAGG
TTGAGAAGGG
AAAGTCTAAG
AAGCTGAAGT
CGGTCAAGGA
ACTGCTCGGG
ATCACCATTA
TGGAGCGCTC
CAGCTTCGAA
AAGAATCCCA
TCGACTTTCT
CGAGGCCAAG
GGCTATAAGG
AAGTCAAGAA
GGATCTTATC
ATTAAGCTGC
CTAAGTACTC
TTTGTTCGAG
CTTGAAAACG
GTCGAAAGCG
AATGCTCGCA
TCGGCAGGAG
AGTTGCAGAA
GGGGAATGAA
TTGGCACTTC
CCTCAAAGTA
CGTGAACTTC
CTGTATCTCG
CGTCCCACTA
CGAGAAGCTG
AAGGGTAGCC
CTGAGGACAA
CGAACAGAAG
CAACTTTTTG
TTGAGCAACA
CAAGCATTAT
CTGGATGAGA
TCATTGAACA
GATTTCAGAG
TTCAGTAAGC
GCGTCATCCT
CGCCGATGCT
AATCTCGACA
AGGTGTTGTC
GGCCTACAAC
AAGCACCGTG
ACAAGCCGAT
CCGAGAGCAG
GCTGAAAATA
TCATTCATCT
GTTCACCCTC
ACTAACTTGG
GAGCACCAGC
AGCGTTCAAG
TATTTTGATA
CGACAATCGA
CCGTAAGCGA
TACACGTCCA
CAAAGGAGGT
GCTTGATGCG
ACCCTGATTC
ATCAATCCAT
CACTGGGCTC
TATGAAACCC
GTATCGACCT
TAGTCAACTG
GGGGGCGACC
CTCCCAAGAA
GAAGCGCAAG
GTCTGAGCGG
ACATTCGATT
TATGCCGTTA
TGACTTCCTT
AAAAAAGCCT
TTACGAATGA
AAGAAATGGA
ATTAGACTTG
TTATGTAGTT
GATTCTACAA
TGGATTATGA
TTCCTGAACT
TCAAATCCGC
TGTTCATTAT
TAATCTCAGC
TCTTCCCGTA
AAGCCAATGT
TGAAACTATT
CGTAAATGTA
CCTCGTTTTG
CGTGTACCTT
GCTTATCACG
TGATATTACA
TGACCTGGAC
AGAGTTCTGC
GCGAAAGTCA
TAACGTAAAT
CCCGGGCGGT
AGGTGCGTCC
CGGGCGGAAG
GTAGTTTTCT
CGTCCACCCC
AACGCGTTTA
TCAACCTCAA
CTTTCAACAA
CCATCATGCC
ACCAAAAGCG
CGTAAAACAA
AGCGAGATTT
GATTGAGCAA
GAGGGCAGGA
TCCAATGCGC
GATTCAAGAC
ATTAAAAATG
GAAAATTTCA
AAAAATTGCG
CCCGCAGCGC
GTGCATACAA
AATTCATCCC
AATACAAGTG
TAATTCCTCG
TGTACTGTGT
AAGCGCCCAC
TAGGTAATAT
GACATGATTA
CGAATTCGAG
CTCGGTACCC
GGCCGGGGAT
CCTCTAGACG
TGGAGTTACC
AGTGATTGAC
CAATGTTTTA
TCTTCTACAG
TTCTGCCTGT
CTACCCCATT
CTAGCTGTAC
CTGACTACAG
AGTAGTTTAA
TTGTGGTTGA
CCCCACAGTC
GGAGGCGGAG
GAATACAGCA
CCGATGTGGC
CTGTCTCCAT
CCAGATTGGC
ACGCAATTTT
TACACGCGGA
AAAGATCGAG
ATAGAGTACG
ACTTTAAATT
TAGTCCCCGG
CGGCTTCTAT
TTTAGAATAT
TTGAGATTTG
ATTCTCAAGC
AATTGATTTG
GTTGGGTCAC
CCTCAATTGG
ATAATATACC
TCATTGCTCG
GCTACTTCAA
CTCATCAATC
ACCGTCATAC
CCCGCATATA
ACCCTCCATT
CCCACGATGT
CGTCCAAGTC
GCAATTGACT
TACGGTGCTC
GAGCCAGCAA
GCACCCCAAT
CCTCTGGCAA
AGAGACTTTT
TGAGATTGCC
GAAGCAAAGA
AGACAAACGT
TACCGTCTCT
GCTGATGTGA
CGACAACCCG
AGAACTCCTG
GACCTCGCTG
ACCGTACGGA
AGCTGTTGGA
TCCAATACAT
ATGCCGTCTA
GCAATGGACT
AATCAACTTT
TGATGATACA
GGTCTCGGTC
CCTACATCGC
CGTCATCAAG
ACACACATCG
ACATCCTCAC
CGATTTCAGC
GTCGACACTA
TCAATGGCCT
GAATGTGCTG
GCTCAAAAGT
ACAACTTTTT
GATCTTCGAG
GACCGCAAAT
TCATCGACAT
CGGCAATACC
GTCCAGAAGC
AATACCACGG
CGGTGCTCTG
AGGATCTCCG
AATGGGCCCA
CATTATCAAC
TGCAGCGTTC
TCCCTGGCGA
GGGCATCGTC
GAGGCTCTGG
CCCAGACCGC
ATCTGCGCAA
GACTTCCCCT
ATGGTCCTGA
GAGAGGACTG
TTGGTCCTGG
CAGAGATGAC
CCCCAAAGGA
TCGCTGGCTA
CGGGCGAGTA
TACCAAGGCA
TCGGTTGACT
ACGCTCGCAA
ATACAAGAAC
TTCGTTATGG
GTTTCGTGTC
GACGCGGGCC
CTGACGGAAG
TGCAGTCGGA
TGTGTCTTCA
GCCTCGGAGG
ATGAAGATTT
CGTGGTCTTC
ACGACGGGTG
TGAACCTCTC
TTCCAAAGGA
GATAAGCTTG
GACAGCAATA
CCAGACTCCT
GCATCGGCTA
TTGGACGCGG
TGCCGACTTT
ATCATCGCCG
GTCGAGGCAT
CTACGCTGCT
CCCGACCCGG
TTGAAGCTGC
ACAGCGGTAC
CAGAAAGAAG
GCTGGGAAGC
TTATATGGCC
AGAGTATGCG
GCAAGTCATG
ATTTCCTCTT
GGAGCAAAAG
TGTAGTGCCA
GTACGAGTGT
TGTGGAGGAA
GGCTGCATAC
ATTGTGCCTG
TCATTAAACG
ATGAGCTCGT
CCGTATTGGC
CCCTGTAATG
CCATGTTTTC
CGCCCCCAAT
CGTCAAGGTT
TTCCCTTTGT
TAGATTCCTA
CCAGTCATCT
AGCAAGGCGG
CCGCAGCTAG
CACAATTGAG
GCATCCCCAC
TACCGCATTA
AGACCTCAGC
GCGGCCGCAA
ATTTAAATAA
AATGAAGTGA
AGTTCCTATA
CTTTCTAGAG
AATAGGAACT
TCTATAGTGA
GTCGAATAAG
GGCGACACAA
AATTTATTCT
AAATGCATAA
TAAATACTGA
TAACATCTTA
TAGTTTGTAT
TATATTTTGT
ATTATCGTTG
ACATGTATAA
TTTTGATATC
AAAAACTGAT
TTTCCCTTTA
TTATTTTCGA
GATTTATTTT
CTTAATTCTC
TTTAACAAAC
TAGAAATATT
GTATATACAA
AAAATCATAA
ATAATAGATG
AATAGTTTAA
TTATAGGTGT
TCATCAATCG
AAAAAGCAAC
GTATCTTATT
TAAAGTGCGT
TGCTTTTTTC
TCATTTATAA
GGTTAAATAA
TTCTCATATA
TCAAGCAAAG
TGACAGGCGC
CCTTAAATAT
TCTGACAAAT
GCTCTTTCCC
TAAACTCCCC
CCATAAAAAA
ACCCGCCGAA
GCGGGTTTTT
ACGTTATTTG
CGGATTAACG
ATTACTCGTT
ATCAGAACCG
CCCAGGGGGC
CCGAGCTTAA
GACTGGCCGT
CGTTTTACAA
CACAGAAAGA
GTTTGTAGAA
ACGCAAAAAG
GCCATCCGTC
AGGGGCCTTC
TGCTTAGTTT
GATGCCTGGC
AGTTCCCTAC
TCTCGCCTTC
CGCTTCCTCG
CTCACTGACT
CGCTGCGCTC
GGTCGTTCGG
CTGCGGCGAG
CGGTATCAGC
TCACTCAAAG
GCGGTAATAC
GGTTATCCAC
AGAATCAGGG
GATAACGCAG
GAAAGAACAT
GTGAGCAAAA
GGCCAGCAAA
AGGCCAGGAA
CCGTAAAAAG
GCCGCGTTGC
TGGCGTTTTT
CCATAGGCTC
CGCCCCCCTG
ACGAGCATCA
CAAAAATCGA
CGCTCAAGTC
AGAGGTGGCG
AAACCCGACA
GGACTATAAA
GATACCAGGC
GTTTCCCCCT
GGAAGCTCCC
TCGTGCGCTC
TCCTGTTCCG
ACCCTGCCGC
TTACCGGATA
CCTGTCCGCC
TTTCTCCCTT
CGGGAAGCGT
GGCGCTTTCT
CATAGCTCAC
GCTGTAGGTA
TCTCAGTTCG
GTGTAGGTCG
TTCGCTCCAA
GCTGGGCTGT
GTGCACGAAC
CCCCCGTTCA
GCCCGACCGC
TGCGCCTTAT
CCGGTAACTA
TCGTCTTGAG
TCCAACCCGG
TAAGACACGA
CTTATCGCCA
CTGGCAGCAG
CCACTGGTAA
CAGGATTAGC
AGAGCGAGGT
ATGTAGGCGG
TGCTACAGAG
TTCTTGAAGT
GGTGGGCTAA
CTACGGCTAC
ACTAGAAGAA
CAGTATTTGG
TATCTGCGCT
CTGCTGAAGC
CAGTTACCTT
CGGAAAAAGA
GTTGGTAGCT
CTTGATCCGG
CAAACAAACC
ACCGCTGGTA
GCGGTGGTTT
TTTTGTTTGC
AAGCAGCAGA
TTACGCGCAG
AAAAAAAGGA
TCTCAAGAAG
ATCCTTTGAT
CTTTTCTACG
GGGTCTGACG
CTCAGTGGAA
CGACGCGCGC
GTAACTCACG
TTAAGGGATT
TTGGTCATGA
GCTTGCGCCG
TCCCGTCAAG
TCAGCGTAAT
GCTCTGCTT