名称: | PiggyBac-hAsCpf1 |
---|---|
别称: | Cpf1酶表达 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑,转座 |
片段类型: | CRISPR |
片段物种: | 蛋白酶 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
反向引物: | EF1a-R:gagccagtacacgacatcac |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
AAATTGTAAG
CGTTAATATT
TTGTTAAAAT
TCGCGTTAAA
TTTTTGTTAA
ATCAGCTCAT
TTTTTAACCA
ATAGGCCGAA
ATCGGCAAAA
TCCCTTATAA
ATCAAAAGAA
TAGACCGAGA
TAGGGTTGAG
TGTTGTTCCA
GTTTGGAACA
AGAGTCCACT
ATTAAAGAAC
GTGGACTCCA
ACGTCAAAGG
GCGAAAAACC
GTCTATCAGG
GCGATGGCCC
ACTACGTGAA
CCATCACCCT
AATCAAGTTT
TTTGGGGTCG
AGGTGCCGTA
AAGCACTAAA
TCGGAACCCT
AAAGGGAGCC
CCCGATTTAG
AGCTTGACGG
GGAAAGCCGG
CGAACGTGGC
GAGAAAGGAA
GGGAAGAAAG
CGAAAGGAGC
GGGCGCTAGG
GCGCTGGCAA
GTGTAGCGGT
CACGCTGCGC
GTAACCACCA
CACCCGCCGC
GCTTAATGCG
CCGCTACAGG
GCGCGTCCCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGCTG
GCGAAAGGGG
GATGTGCTGC
AAGGCGATTA
AGTTGGGTAA
CGCCAGGGTT
TTCCCAGTCA
CGACGTTGTA
AAACGACGGC
CAGTGAGCGC
GCCTCGTTCA
TTCACGTTTT
TGAACCCGTG
GAGGACGGGC
AGACTCGCGG
TGCAAATGTG
TTTTACAGCG
TGATGGAGCA
GATGAAGATG
CTCGACACGC
TGCAGAACAC
GCAGCTAGAT
TAACCCTAGA
AAGATAATCA
TATTGTGACG
TACGTTAAAG
ATAATCATGC
GTAAAATTGA
CGCATGTGTT
TTATCGGTCT
GTATATCGAG
GTTTATTTAT
TAATTTGAAT
AGATATTAAG
TTTTATTATA
TTTACACTTA
CATACTAATA
ATAAATTCAA
CAAACAATTT
ATTTATGTTT
ATTTATTTAT
TAAAAAAAAA
CAAAAACTCA
AAATTTCTTC
TATAAAGTAA
CAAAACTTTT
ATGAGGGACA
GCCCCCCCCC
AAAGCCCCCA
GGGATGTAAT
TACGTCCCTC
CCCCGCTAGG
GGGCAGCAGC
GAGCCGCCCG
GGGCTCCGCT
CCGGTCCGGC
GCTCCCCCCG
CATCCCCGAG
CCGGCAGCGT
GCGGGGACAG
CCCGGGCACG
GGGAAGGTGG
CACGGGATCG
CTTTCCTCTG
AACGCTTCTC
GCTGCTCTTT
GAGCCTGCAG
ACACCTGGGG
GGATACGGGG
AAAAGGCCTC
CAAGGCCTAC
TAGTATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GAGCTCGTTT
AGTGAACCGT
CAGATCGCCT
GGAGACGCCA
TCCACGCTGT
TTTGACCTCC
ATAGAAGATT
CTAGAGCTAG
CGTTTAAACT
TAAGCTTGGT
ACCGCCACCA
TGACACAGTT
CGAGGGCTTT
ACCAACCTGT
ATCAGGTGAG
CAAGACACTG
CGGTTTGAGC
TGATCCCACA
GGGCAAGACC
CTGAAGCACA
TCCAGGAGCA
GGGCTTCATC
GAGGAGGACA
AGGCCCGCAA
TGATCACTAC
AAGGAGCTGA
AGCCCATCAT
CGATCGGATC
TACAAGACCT
ATGCCGACCA
GTGCCTGCAG
CTGGTGCAGC
TGGATTGGGA
GAACCTGAGC
GCCGCCATCG
ACTCCTATAG
AAAGGAGAAA
ACCGAGGAGA
CAAGGAACGC
CCTGATCGAG
GAGCAGGCCA
CATATCGCAA
TGCCATCCAC
GACTACTTCA
TCGGCCGGAC
AGACAACCTG
ACCGATGCCA
TCAATAAGAG
ACACGCCGAG
ATCTACAAGG
GCCTGTTCAA
GGCCGAGCTG
TTTAATGGCA
AGGTGCTGAA
GCAGCTGGGC
ACCGTGACCA
CAACCGAGCA
CGAGAACGCC
CTGCTGCGGA
GCTTCGACAA
GTTTACAACC
TACTTCTCCG
GCTTTTATGA
GAACAGGAAG
AACGTGTTCA
GCGCCGAGGA
TATCAGCACA
GCCATCCCAC
ACCGCATCGT
GCAGGACAAC
TTCCCCAAGT
TTAAGGAGAA
TTGTCACATC
TTCACACGCC
TGATCACCGC
CGTGCCCAGC
CTGCGGGAGC
ACTTTGAGAA
CGTGAAGAAG
GCCATCGGCA
TCTTCGTGAG
CACCTCCATC
GAGGAGGTGT
TTTCCTTCCC
TTTTTATAAC
CAGCTGCTGA
CACAGACCCA
GATCGACCTG
TATAACCAGC
TGCTGGGAGG
AATCTCTCGG
GAGGCAGGCA
CCGAGAAGAT
CAAGGGCCTG
AACGAGGTGC
TGAATCTGGC
CATCCAGAAG
AATGATGAGA
CAGCCCACAT
CATCGCCTCC
CTGCCACACA
GATTCATCCC
CCTGTTTAAG
CAGATCCTGT
CCGATAGGAA
CACCCTGTCT
TTCATCCTGG
AGGAGTTTAA
GAGCGACGAG
GAAGTGATCC
AGTCCTTCTG
CAAGTACAAG
ACACTGCTGA
GAAACGAGAA
CGTGCTGGAG
ACAGCCGAGG
CCCTGTTTAA
CGAGCTGAAC
AGCATCGACC
TGACACACAT
CTTCATCAGC
CACAAGAAGC
TGGAGACAAT
CAGCAGCGCC
CTGTGCGACC
ACTGGGATAC
ACTGAGGAAT
GCCCTGTATG
AGCGGAGAAT
CTCCGAGCTG
ACAGGCAAGA
TCACCAAGTC
TGCCAAGGAG
AAGGTGCAGC
GCAGCCTGAA
GCACGAGGAT
ATCAACCTGC
AGGAGATCAT
CTCTGCCGCA
GGCAAGGAGC
TGAGCGAGGC
CTTCAAGCAG
AAAACCAGCG
AGATCCTGTC
CCACGCACAC
GCCGCCCTGG
ATCAGCCACT
GCCTACAACC
CTGAAGAAGC
AGGAGGAGAA
GGAGATCCTG
AAGTCTCAGC
TGGACAGCCT
GCTGGGCCTG
TACCACCTGC
TGGACTGGTT
TGCCGTGGAT
GAGTCCAACG
AGGTGGACCC
CGAGTTCTCT
GCCCGGCTGA
CCGGCATCAA
GCTGGAGATG
GAGCCTTCTC
TGAGCTTCTA
CAACAAGGCC
AGAAATTATG
CCACCAAGAA
GCCCTACTCC
GTGGAGAAGT
TCAAGCTGAA
CTTTCAGATG
CCTACACTGG
CCTCTGGCTG
GGACGTGAAT
AAGGAGAAGA
ACAATGGCGC
CATCCTGTTT
GTGAAGAACG
GCCTGTACTA
TCTGGGCATC
ATGCCAAAGC
AGAAGGGCAG
GTATAAGGCC
CTGAGCTTCG
AGCCCACAGA
GAAAACCAGC
GAGGGCTTTG
ATAAGATGTA
CTATGACTAC
TTCCCTGATG
CCGCCAAGAT
GATCCCAAAG
TGCAGCACCC
AGCTGAAGGC
CGTGACAGCC
CACTTTCAGA
CCCACACAAC
CCCCATCCTG
CTGTCCAACA
ATTTCATCGA
GCCTCTGGAG
ATCACAAAGG
AGATCTACGA
CCTGAACAAT
CCTGAGAAGG
AGCCAAAGAA
GTTTCAGACA
GCCTACGCCA
AGAAAACCGG
CGACCAGAAG
GGCTACAGAG
AGGCCCTGTG
CAAGTGGATC
GACTTCACAA
GGGATTTTCT
GTCCAAGTAT
ACCAAGACAA
CCTCTATCGA
TCTGTCTAGC
CTGCGGCCAT
CCTCTCAGTA
TAAGGACCTG
GGCGAGTACT
ATGCCGAGCT
GAATCCCCTG
CTGTACCACA
TCAGCTTCCA
GAGAATCGCC
GAGAAGGAGA
TCATGGATGC
CGTGGAGACA
GGCAAGCTGT
ACCTGTTCCA
GATCTATAAC
AAGGACTTTG
CCAAGGGCCA
CCACGGCAAG
CCTAATCTGC
ACACACTGTA
TTGGACCGGC
CTGTTTTCTC
CAGAGAACCT
GGCCAAGACA
AGCATCAAGC
TGAATGGCCA
GGCCGAGCTG
TTCTACCGCC
CTAAGTCCAG
GATGAAGAGG
ATGGCACACC
GGCTGGGAGA
GAAGATGCTG
AACAAGAAGC
TGAAGGATCA
GAAAACCCCA
ATCCCCGACA
CCCTGTACCA
GGAGCTGTAC
GACTATGTGA
ATCACAGACT
GTCCCACGAC
CTGTCTGATG
AGGCCAGGGC
CCTGCTGCCC
AACGTGATCA
CCAAGGAGGT
GTCTCACGAG
ATCATCAAGG
ATAGGCGCTT
TACCAGCGAC
AAGTTCTTTT
TCCACGTGCC
TATCACACTG
AACTATCAGG
CCGCCAATTC
CCCATCTAAG
TTCAACCAGA
GGGTGAATGC
CTACCTGAAG
GAGCACCCCG
AGACACCTAT
CATCGGCATC
GATCGGGGCG
AGAGAAACCT
GATCTATATC
ACAGTGATCG
ACTCCACCGG
CAAGATCCTG
GAGCAGCGGA
GCCTGAACAC
CATCCAGCAG
TTTGATTACC
AGAAGAAGCT
GGACAACAGG
GAGAAGGAGA
GGGTGGCAGC
AAGGCAGGCC
TGGTCTGTGG
TGGGCACAAT
CAAGGATCTG
AAGCAGGGCT
ATCTGAGCCA
GGTCATCCAC
GAGATCGTGG
ACCTGATGAT
CCACTACCAG
GCCGTGGTGG
TGCTGGAGAA
CCTGAATTTC
GGCTTTAAGA
GCAAGAGGAC
CGGCATCGCC
GAGAAGGCCG
TGTACCAGCA
GTTCGAGAAG
ATGCTGATCG
ATAAGCTGAA
TTGCCTGGTG
CTGAAGGACT
ATCCAGCAGA
GAAAGTGGGA
GGCGTGCTGA
ACCCATACCA
GCTGACAGAC
CAGTTCACCT
CCTTTGCCAA
GATGGGCACC
CAGTCTGGCT
TCCTGTTTTA
CGTGCCTGCC
CCATATACAT
CTAAGATCGA
TCCCCTGACC
GGCTTCGTGG
ACCCCTTCGT
GTGGAAAACC
ATCAAGAATC
ACGAGAGCCG
CAAGCACTTC
CTGGAGGGCT
TCGACTTTCT
GCACTACGAC
GTGAAAACCG
GCGACTTCAT
CCTGCACTTT
AAGATGAACA
GAAATCTGTC
CTTCCAGAGG
GGCCTGCCCG
GCTTTATGCC
TGCATGGGAT
ATCGTGTTCG
AGAAGAACGA
GACACAGTTT
GACGCCAAGG
GCACCCCTTT
CATCGCCGGC
AAGAGAATCG
TGCCAGTGAT
CGAGAATCAC
AGATTCACCG
GCAGATACCG
GGACCTGTAT
CCTGCCAACG
AGCTGATCGC
CCTGCTGGAG
GAGAAGGGCA
TCGTGTTCAG
GGATGGCTCC
AACATCCTGC
CAAAGCTGCT
GGAGAATGAC
GATTCTCACG
CCATCGACAC
CATGGTGGCC
CTGATCCGCA
GCGTGCTGCA
GATGCGGAAC
TCCAATGCCG
CCACAGGCGA
GGACTATATC
AACAGCCCCG
TGCGCGATCT
GAATGGCGTG
TGCTTCGACT
CCCGGTTTCA
GAACCCAGAG
TGGCCCATGG
ACGCCGATGC
CAATGGCGCC
TACCACATCG
CCCTGAAGGG
CCAGCTGCTG
CTGAATCACC
TGAAGGAGAG
CAAGGATCTG
AAGCTGCAGA
ACGGCATCTC
CAATCAGGAC
TGGCTGGCCT
ACATCCAGGA
GCTGCGCAAC
AAAAGGCCGG
CGGCCACGAA
AAAGGCCGGC
CAGGCAAAAA
AGAAAAAGGG
ATCCTACCCA
TACGATGTTC
CAGATTACGC
TTATCCCTAC
GACGTGCCTG
ATTATGCATA
CCCATATGAT
GTCCCCGACT
ATGCCTAAGA
ATTCGAATTT
AAATCGGATC
CGCGGCCGCA
AGGATCTGCG
ATCGCTCCGG
TGCCCGTCAG
TGGGCAGAGC
GCACATCGCC
CACAGTCCCC
GAGAAGTTGG
GGGGAGGGGT
CGGCAATTGA
ACGGGTGCCT
AGAGAAGGTG
GCGCGGGGTA
AACTGGGAAA
GTGATGTCGT
GTACTGGCTC
CGCCTTTTTC
CCGAGGGTGG
GGGAGAACCG
TATATAAGTG
CAGTAGTCGC
CGTGAACGTT
CTTTTTCGCA
ACGGGTTTGC
CGCCAGAACA
CAGCTGAAGC
TTCGAGGGGC
TCGCATCTCT
CCTTCACGCG
CCCGCCGCCC
TACCTGAGGC
CGCCATCCAC
GCCGGTTGAG
TCGCGTTCTG
CCGCCTCCCG
CCTGTGGTGC
CTCCTGAACT
GCGTCCGCCG
TCTAGGTAAG
TTTAAAGCTC
AGGTCGAGAC
CGGGCCTTTG
TCCGGCGCTC
CCTTGGAGCC
TACCTAGACT
CAGCCGGCTC
TCCACGCTTT
GCCTGACCCT
GCTTGCTCAA
CTCTACGTCT
TTGTTTCGTT
TTCTGTTCTG
CGCCGTTACA
GATCCAAGCT
GTGACCGGCG
CCTACGCTAG
ACGCCACCAT
GGAGAGCGAC
GAGAGCGGCC
TGCCCGCCAT
GGAGATCGAG
TGCCGCATCA
CCGGCACCCT
GAACGGCGTG
GAGTTCGAGC
TGGTGGGCGG
CGGAGAGGGC
ACCCCCAAGC
AGGGCCGCAT
GACCAACAAG
ATGAAGAGCA
CCAAAGGCGC
CCTGACCTTC
AGCCCCTACC
TGCTGAGCCA
CGTGATGGGC
TACGGCTTCT
ACCACTTCGG
CACCTACCCC
AGCGGCTACG
AGAACCCCTT
CCTGCACGCC
ATCAACAACG
GCGGCTACAC
CAACACCCGC
ATCGAGAAGT
ACGAGGACGG
CGGCGTGCTG
CACGTGAGCT
TCAGCTACCG
CTACGAGGCC
GGCCGCGTGA
TCGGCGACTT
CAAGGTGGTG
GGCACCGGCT
TCCCCGAGGA
CAGCGTGATC
TTCACCGACA
AGATCATCCG
CAGCAACGCC
ACCGTGGAGC
ACCTGCACCC
CATGGGCGAT
AACGTGCTGG
TGGGCAGCTT
CGCCCGCACC
TTCAGCCTGC
GCGACGGCGG
CTACTACAGC
TTCGTGGTGG
ACAGCCACAT
GCACTTCAAG
AGCGCCATCC
ACCCCAGCAT
CCTGCAGAAC
GGGGGCCCCA
TGTTCGCCTT
CCGCCGCGTG
GAGGAGCTGC
ACAGCAACAC
CGAGCTGGGC
ATCGTGGAGT
ACCAGCACGC
CTTCAAGACC
CCCATCGCCT
TCGCCAGATC
CCGCGCTCAG
TCGTCCAATT
CTGCCGTGGA
CGGCACCGCC
GGACCCGGCT
CCACCGGATC
TCGCGAGGGC
AGAGGAAGTC
TTCTAACATG
CGGTGACGTG
GAGGAGAATC
CCGGCCCTAT
GACCGAGTAC
AAGCCCACGG
TGCGCCTCGC
CACCCGCGAC
GACGTCCCCA
GGGCCGTACG
CACCCTCGCC
GCCGCGTTCG
CCGACTACCC
CGCCACGCGC
CACACCGTCG
ATCCGGACCG
CCACATCGAG
CGGGTCACCG
AGCTGCAAGA
ACTCTTCCTC
ACGCGCGTCG
GGCTCGACAT
CGGCAAGGTG
TGGGTCGCGG
ACGACGGCGC
CGCGGTGGCG
GTCTGGACCA
CGCCGGAGAG
CGTCGAAGCG
GGGGCGGTGT
TCGCCGAGAT
CGGCCCGCGC
ATGGCCGAGT
TGAGCGGTTC
CCGGCTGGCC
GCGCAGCAAC
AGATGGAAGG
CCTCCTGGCG
CCGCACCGGC
CCAAGGAGCC
CGCGTGGTTC
CTGGCCACCG
TCGGCGTCTC
GCCCGACCAC
CAGGGCAAGG
GTCTGGGCAG
CGCCGTCGTG
CTCCCCGGAG
TGGAGGCGGC
CGAGCGCGCC
GGGGTGCCCG
CCTTCCTGGA
GACCTCCGCG
CCCCGCAACC
TCCCCTTCTA
CGAGCGGCTC
GGCTTCACCG
TCACCGCCGA
CGTCGAGGTG
CCCGAAGGAC
CGCGCACCTG
GTGCATGACC
CGCAAGCCCG
GTGCCTGAAA
TCAACCTCTG
GATTACAAAA
TTTGTGAAAG
ATTGACTGGT
ATTCTTAACT
ATGTTGCTCC
TTTTACGCTA
TGTGGATACG
CTGCTTTAAT
GCCTTTGTAT
CAGTTAACTT
GTTTATTGCA
GCTTATAATG
GTTACAAATA
AAGCAATAGC
ATCACAAATT
TCACAAATAA
AGCATTTTTT
TCACTGCATT
CTAGTTGTGG
TTTGTCCAAA
CTCATCAATG
TATCTTATCA
TGTCTGGAAT
TGACTCAAAT
GATGTCAATT
AGTCTATCAG
AAGCTATCTG
GTCTCCCTTC
CGGGGGACAA
GACATCCCTG
TTTAATATTT
AAACAGCAGT
GTTCCCAAAC
TGGGTTCTTA
TATCCCTTGC
TCTGGTCAAC
CAGGTTGCAG
GGTTTCCTGT
CCTCACAGGA
ACGAAGTCCC
TAAAGAAACA
GTGGCAGCCA
GGTTTAGCCC
CGGAATTGAC
TGGATTCCTT
TTTTAGGGCC
CATTGGTATG
GCTTTTTCCC
CGTATCCCCC
CAGGTGTCTG
CAGGCTCAAA
GAGCAGCGAG
AAGCGTTCAG
AGGAAAGCGA
TCCCGTGCCA
CCTTCCCCGT
GCCCGGGCTG
TCCCCGCACG
CTGCCGGCTC
GGGGATGCGG
GGGGAGCGCC
GGACCGGAGC
GGAGCCCCGG
GCGGCTCGCT
GCTGCCCCCT
AGCGGGGGAG
GGACGTAATT
ACATCCCTGG
GGGCTTTGGG
GGGGGGCTGT
CCCTGATATC
TATAACAAGA
AAATATATAT
ATAATAAGTT
ATCACGTAAG
TAGAACATGA
AATAACAATA
TAATTATCGT
ATGAGTTAAA
TCTTAAAAGT
CACGTAAAAG
ATAATCATGC
GTCATTTTGA
CTCACGCGGT
CGTTATAGTT
CAAAATCAGT
GACACTTACC
GCATTGACAA
GCACGCCTCA
CGGGAGCTCC
AAGCGGCGAC
TGAGATGTCC
TAAATGCACA
GCGACGGATT
CGCGCTATTT
AGAAAGAGAG
AGCAATATTT
CAAGAATGCA
TGCGTCAATT
TTACGCAGAC
TATCTTTCTA
GGGTTAATCT
AGCTGCATCA
GGATCATATC
GTCGGGTCTT
TTTTCCGGCT
CAGTCATCGC
CCAAGCTGGC
GCTATCTGGG
CATCGGGGAG
GAAGAAGCCC
GTGCCTTTTC
CCGCGAGGTT
GAAGCGGCAT
GGAAAGAGTT
TGCCGAGGAT
GACTGCTGCT
GCATTGACGT
TGAGCGAAAA
CGCACGTTTA
CCATGATGAT
TCGGGAAGGT
GTGGCCATGC
ACGCCTTTAA
CGGTGAACTG
TTCGTTCAGG
CCACCTGGGA
TACCAGTTCG
TCGCGGCTTT
TCCGGACACA
GTTCCGGATG
GTCAGCCCGA
AGCGCATCAG
CAACCCGAAC
AATACCGGCG
ACAGCCGGAA
CTGCCGTGCC
GGTGTGCAGA
TTAATGACAG
CGGTGCGGCG
CTGGGATATT
ACGTCAGCGA
GGACGGGTAT
CCTGGCTGGA
TGCCGCAGAA
ATGGACATGG
ATACCCCGTG
AGTTACCCGG
CGGGCGCGCT
TGGCGTAATC
ATGGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGGACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
ACTTGGTCTG
ACAGTTACCA
ATGCTTAATC
AGTGAGGCAC
CTATCTCAGC
GATCTGTCTA
TTTCGTTCAT
CCATAGTTGC
CTGACTCCCC
GTCGTGTAGA
TAACTACGAT
ACGGGAGGGC
TTACCATCTG
GCCCCAGTGC
TGCAATGATA
CCGCGAGACC
CACGCTCACC
GGCTCCAGAT
TTATCAGCAA
TAAACCAGCC
AGCCGGAAGG
GCCGAGCGCA
GAAGTGGTCC
TGCAACTTTA
TCCGCCTCCA
TCCAGTCTAT
TAATTGTTGC
CGGGAAGCTA
GAGTAAGTAG
TTCGCCAGTT
AATAGTTTGC
GCAACGTTGT
TGCCATTGCT
ACAGGCATCG
TGGTGTCACG
CTCGTCGTTT
GGTATGGCTT
CATTCAGCTC
CGGTTCCCAA
CGATCAAGGC
GAGTTACATG
ATCCCCCATG
TTGTGCAAAA
AAGCGGTTAG
CTCCTTCGGT
CCTCCGATCG
TTGTCAGAAG
TAAGTTGGCC
GCAGTGTTAT
CACTCATGGT
TATGGCAGCA
CTGCATAATT
CTCTTACTGT
CATGCCATCC
GTAAGATGCT
TTTCTGTGAC
TGGTGAGTAC
TCAACCAAGT
CATTCTGAGA
ATAGTGTATG
CGGCGACCGA
GTTGCTCTTG
CCCGGCGTCA
ATACGGGATA
ATACCGCGCC
ACATAGCAGA
ACTTTAAAAG
TGCTCATCAT
TGGAAAACGT
TCTTCGGGGC
GAAAACTCTC
AAGGATCTTA
CCGCTGTTGA
GATCCAGTTC
GATGTAACCC
ACTCGTGCAC
CCAACTGATC
TTCAGCATCT
TTTACTTTCA
CCAGCGTTTC
TGGGTGAGCA
AAAACAGGAA
GGCAAAATGC
CGCAAAAAAG
GGAATAAGGG
CGACACGGAA
ATGTTGAATA
CTCAT