名称: | pLV3-CMV-FLAG-BirA(humanized)-Cas9-Blast |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达,基因编辑 |
片段类型: | ORF,CRISPR,Cas9 |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Blast |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTTTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGATTCT
AGAGCCACCA
TGGACTACAA
AGACGATGAC
GACAAGGGCT
CTAAAGACAA
TACTGTGCCT
CTGAAGCTGA
TCGCTCTCCT
GGCTAATGGC
GAGTTCCATA
GTGGCGAACA
GCTGGGAGAA
ACCCTGGGCA
TGTCCAGGGC
CGCTATCAAC
AAGCACATTC
AGACTCTGCG
CGACTGGGGC
GTGGACGTGT
TCACCGTGCC
CGGAAAGGGC
TACTCTCTGC
CCGAGCCTAT
CCCGCTGCTG
AACGCTAAAC
AGATTCTGGG
ACAGCTGGAC
GGCGGGAGCG
TGGCAGTCCT
GCCTGTGGTC
GACTCCACCA
ATCAGTACCT
GCTGGATCGA
ATCGGCGAGC
TGAAGAGTGG
GGATGCTTGC
ATTGCAGAAT
ATCAGCAGGC
AGGGAGAGGA
AGCAGAGGGA
GGAAATGGTT
CTCTCCTTTT
GGAGCTAACC
TGTACCTGAG
TATGTTTTGG
CGCCTGAAGC
GGGGACCAGC
AGCAATCGGC
CTGGGCCCGG
TCATCGGAAT
TGTCATGGCA
GAAGCGCTGC
GAAAGCTGGG
AGCAGACAAG
GTGCGAGTCA
AATGGCCCAA
TGACCTGTAT
CTGCAGGATA
GAAAGCTGGC
AGGCATCCTG
GTGGAGCTGG
CCGGAATAAC
AGGGCGCGCC
TCTGGAGGAT
CTAGCGGAGG
ATCCTCTGGC
AGCGAGACAC
CAGGAACAAG
CGAGTCAGCA
ACACCAGAGA
GCAGTGGCGG
CAGCAGCGGC
GGCAGCATGG
ACAAGAAGTA
CAGCATCGGC
CTGGACATCG
GCACCAACTC
TGTGGGCTGG
GCCGTGATCA
CCGACGAGTA
CAAGGTGCCC
AGCAAGAAAT
TCAAGGTGCT
GGGCAACACC
GACCGGCACA
GCATCAAGAA
GAACCTGATC
GGAGCCCTGC
TGTTCGACAG
CGGCGAAACA
GCCGAGGCCA
CCCGGCTGAA
GAGAACCGCC
AGAAGAAGAT
ACACCAGACG
GAAGAACCGG
ATCTGCTATC
TGCAAGAGAT
CTTCAGCAAC
GAGATGGCCA
AGGTGGACGA
CAGCTTCTTC
CACAGACTGG
AAGAGTCCTT
CCTGGTGGAA
GAGGATAAGA
AGCACGAGCG
GCACCCCATC
TTCGGCAACA
TCGTGGACGA
GGTGGCCTAC
CACGAGAAGT
ACCCCACCAT
CTACCACCTG
AGAAAGAAAC
TGGTGGACAG
CACCGACAAG
GCCGACCTGC
GGCTGATCTA
TCTGGCCCTG
GCCCACATGA
TCAAGTTCCG
GGGCCACTTC
CTGATCGAGG
GCGACCTGAA
CCCCGACAAC
AGCGACGTGG
ACAAGCTGTT
CATCCAGCTG
GTGCAGACCT
ACAACCAGCT
GTTCGAGGAA
AACCCCATCA
ACGCCAGCGG
CGTGGACGCC
AAGGCCATCC
TGTCTGCCAG
ACTGAGCAAG
AGCAGACGGC
TGGAAAATCT
GATCGCCCAG
CTGCCCGGCG
AGAAGAAGAA
TGGCCTGTTC
GGAAACCTGA
TTGCCCTGAG
CCTGGGCCTG
ACCCCCAACT
TCAAGAGCAA
CTTCGACCTG
GCCGAGGATG
CCAAACTGCA
GCTGAGCAAG
GACACCTACG
ACGACGACCT
GGACAACCTG
CTGGCCCAGA
TCGGCGACCA
GTACGCCGAC
CTGTTTCTGG
CCGCCAAGAA
CCTGTCCGAC
GCCATCCTGC
TGAGCGACAT
CCTGAGAGTG
AACACCGAGA
TCACCAAGGC
CCCCCTGAGC
GCCTCTATGA
TCAAGAGATA
CGACGAGCAC
CACCAGGACC
TGACCCTGCT
GAAAGCTCTC
GTGCGGCAGC
AGCTGCCTGA
GAAGTACAAA
GAGATTTTCT
TCGACCAGAG
CAAGAACGGC
TACGCCGGCT
ACATTGACGG
CGGAGCCAGC
CAGGAAGAGT
TCTACAAGTT
CATCAAGCCC
ATCCTGGAAA
AGATGGACGG
CACCGAGGAA
CTGCTCGTGA
AGCTGAACAG
AGAGGACCTG
CTGCGGAAGC
AGCGGACCTT
CGACAACGGC
AGCATCCCCC
ACCAGATCCA
CCTGGGAGAG
CTGCACGCCA
TTCTGCGGCG
GCAGGAAGAT
TTTTACCCAT
TCCTGAAGGA
CAACCGGGAA
AAGATCGAGA
AGATCCTGAC
CTTCCGCATC
CCCTACTACG
TGGGCCCTCT
GGCCAGGGGA
AACAGCAGAT
TCGCCTGGAT
GACCAGAAAG
AGCGAGGAAA
CCATCACCCC
CTGGAACTTC
GAGGAAGTGG
TGGACAAGGG
CGCTTCCGCC
CAGAGCTTCA
TCGAGCGGAT
GACCAACTTC
GATAAGAACC
TGCCCAACGA
GAAGGTGCTG
CCCAAGCACA
GCCTGCTGTA
CGAGTACTTC
ACCGTGTATA
ACGAGCTGAC
CAAAGTGAAA
TACGTGACCG
AGGGAATGAG
AAAGCCCGCC
TTCCTGAGCG
GCGAGCAGAA
AAAGGCCATC
GTGGACCTGC
TGTTCAAGAC
CAACCGGAAA
GTGACCGTGA
AGCAGCTGAA
AGAGGACTAC
TTCAAGAAAA
TCGAGTGCTT
CGACTCCGTG
GAAATCTCCG
GCGTGGAAGA
TCGGTTCAAC
GCCTCCCTGG
GCACATACCA
CGATCTGCTG
AAAATTATCA
AGGACAAGGA
CTTCCTGGAC
AATGAGGAAA
ACGAGGACAT
TCTGGAAGAT
ATCGTGCTGA
CCCTGACACT
GTTTGAGGAC
AGAGAGATGA
TCGAGGAACG
GCTGAAAACC
TATGCCCACC
TGTTCGACGA
CAAAGTGATG
AAGCAGCTGA
AGCGGCGGAG
ATACACCGGC
TGGGGCAGGC
TGAGCCGGAA
GCTGATCAAC
GGCATCCGGG
ACAAGCAGTC
CGGCAAGACA
ATCCTGGATT
TCCTGAAGTC
CGACGGCTTC
GCCAACAGAA
ACTTCATGCA
GCTGATCCAC
GACGACAGCC
TGACCTTTAA
AGAGGACATC
CAGAAAGCCC
AGGTGTCCGG
CCAGGGCGAT
AGCCTGCACG
AGCACATTGC
CAATCTGGCC
GGCAGCCCCG
CCATTAAGAA
GGGCATCCTG
CAGACAGTGA
AGGTGGTGGA
CGAGCTCGTG
AAAGTGATGG
GCCGGCACAA
GCCCGAGAAC
ATCGTGATCG
AAATGGCCAG
AGAGAACCAG
ACCACCCAGA
AGGGACAGAA
GAACAGCCGC
GAGAGAATGA
AGCGGATCGA
AGAGGGCATC
AAAGAGCTGG
GCAGCCAGAT
CCTGAAAGAA
CACCCCGTGG
AAAACACCCA
GCTGCAGAAC
GAGAAGCTGT
ACCTGTACTA
CCTGCAGAAT
GGGCGGGATA
TGTACGTGGA
CCAGGAACTG
GACATCAACC
GGCTGTCCGA
CTACGATGTG
GACCATATCG
TGCCTCAGAG
CTTTCTGAAG
GACGACTCCA
TCGACAACAA
GGTGCTGACC
AGAAGCGACA
AGAACCGGGG
CAAGAGCGAC
AACGTGCCCT
CCGAAGAGGT
CGTGAAGAAG
ATGAAGAACT
ACTGGCGGCA
GCTGCTGAAC
GCCAAGCTGA
TTACCCAGAG
AAAGTTCGAC
AATCTGACCA
AGGCCGAGAG
AGGCGGCCTG
AGCGAACTGG
ATAAGGCCGG
CTTCATCAAG
AGACAGCTGG
TGGAAACCCG
GCAGATCACA
AAGCACGTGG
CACAGATCCT
GGACTCCCGG
ATGAACACTA
AGTACGACGA
GAATGACAAG
CTGATCCGGG
AAGTGAAAGT
GATCACCCTG
AAGTCCAAGC
TGGTGTCCGA
TTTCCGGAAG
GATTTCCAGT
TTTACAAAGT
GCGCGAGATC
AACAACTACC
ACCACGCCCA
CGACGCCTAC
CTGAACGCCG
TCGTGGGAAC
CGCCCTGATC
AAAAAGTACC
CTAAGCTGGA
AAGCGAGTTC
GTGTACGGCG
ACTACAAGGT
GTACGACGTG
CGGAAGATGA
TCGCCAAGAG
CGAGCAGGAA
ATCGGCAAGG
CTACCGCCAA
GTACTTCTTC
TACAGCAACA
TCATGAACTT
TTTCAAGACC
GAGATTACCC
TGGCCAACGG
CGAGATCCGG
AAGCGGCCTC
TGATCGAGAC
AAACGGCGAA
ACCGGGGAGA
TCGTGTGGGA
TAAGGGCCGG
GATTTTGCCA
CCGTGCGGAA
AGTGCTGAGC
ATGCCCCAAG
TGAATATCGT
GAAAAAGACC
GAGGTGCAGA
CAGGCGGCTT
CAGCAAAGAG
TCTATCCTGC
CCAAGAGGAA
CAGCGATAAG
CTGATCGCCA
GAAAGAAGGA
CTGGGACCCT
AAGAAGTACG
GCGGCTTCGA
CAGCCCCACC
GTGGCCTATT
CTGTGCTGGT
GGTGGCCAAA
GTGGAAAAGG
GCAAGTCCAA
GAAACTGAAG
AGTGTGAAAG
AGCTACTGGG
GATCACCATC
ATGGAAAGAA
GCAGCTTCGA
GAAGAATCCC
ATCGACTTTC
TGGAAGCCAA
GGGCTACAAA
GAAGTGAAAA
AGGACCTGAT
CATCAAGCTG
CCTAAGTACT
CCCTGTTCGA
GCTGGAAAAC
GGCCGGAAGA
GAATGCTGGC
CTCTGCCGGC
GAACTGCAGA
AGGGAAACGA
ACTGGCCCTG
CCCTCCAAAT
ATGTGAACTT
CCTGTACCTG
GCCAGCCACT
ATGAGAAGCT
GAAGGGCTCC
CCCGAGGATA
ATGAGCAGAA
ACAGCTGTTT
GTGGAACAGC
ACAAGCACTA
CCTGGACGAG
ATCATCGAGC
AGATCAGCGA
GTTCTCCAAG
AGAGTGATCC
TGGCCGACGC
TAATCTGGAC
AAAGTGCTGT
CCGCCTACAA
CAAGCACCGG
GATAAGCCCA
TCAGAGAGCA
GGCCGAGAAT
ATCATCCACC
TGTTTACCCT
GACCAATCTG
GGAGCCCCTG
CCGCCTTCAA
GTACTTTGAC
ACCACCATCG
ACCGGAAGAG
GTACACCAGC
ACCAAAGAGG
TGCTGGACGC
CACCCTGATC
CACCAGAGCA
TCACCGGCCT
GTACGAGACA
CGGATCGACC
TGTCTCAGCT
GGGAGGCGAC
AAGCGACCTG
CCGCCACAAA
GAAGGCTGGA
CAGGCTAAGA
AGAAGAAATG
AGCGGCCGCG
AAGGATCTGC
GATCGCTCCG
GTGCCCGTCA
GTGGGCAGAG
CGCACATCGC
CCACAGTCCC
CGAGAAGTTG
GGGGGAGGGG
TCGGCAATTG
AACGGGTGCC
TAGAGAAGGT
GGCGCGGGGT
AAACTGGGAA
AGTGATGTCG
TGTACTGGCT
CCGCCTTTTT
CCCGAGGGTG
GGGGAGAACC
GTATATAAGT
GCAGTAGTCG
CCGTGAACGT
TCTTTTTCGC
AACGGGTTTG
CCGCCAGAAC
ACAGCTGAAG
CTTCGAGGGG
CTCGCATCTC
TCCTTCACGC
GCCCGCCGCC
CTACCTGAGG
CCGCCATCCA
CGCCGGTTGA
GTCGCGTTCT
GCCGCCTCCC
GCCTGTGGTG
CCTCCTGAAC
TGCGTCCGCC
GTCTAGGTAA
GTTTAAAGCT
CAGGTCGAGA
CCGGGCCTTT
GTCCGGCGCT
CCCTTGGAGC
CTACCTAGAC
TCAGCCGGCT
CTCCACGCTT
TGCCTGACCC
TGCTTGCTCA
ACTCTACGTC
TTTGTTTCGT
TTTCTGTTCT
GCGCCGTTAC
AGATCCAAGC
TGTGACCGGC
GCCTACGCTA
GGCCACCATG
GCCAAGCCTT
TGTCTCAAGA
AGAATCCACC
CTCATTGAAA
GAGCAACGGC
TACAATCAAC
AGCATCCCCA
TCTCTGAAGA
CTACAGCGTC
GCCAGCGCAG
CTCTCTCTAG
CGACGGCCGC
ATCTTCACTG
GTGTCAATGT
ATATCATTTT
ACTGGGGGAC
CTTGTGCAGA
ACTCGTGGTG
CTGGGCACTG
CTGCTGCTGC
GGCAGCTGGC
AACCTGACTT
GTATCGTCGC
GATCGGAAAT
GAGAACAGGG
GCATCTTGAG
CCCCTGCGGA
CGGTGCCGAC
AGGTGCTTCT
CGATCTGCAT
CCTGGGATCA
AAGCCATAGT
GAAGGACAGT
GATGGACAGC
CGACGGCAGT
TGGGATTCGT
GAATTGCTGC
CCTCTGGTTA
TGTGTGGGAG
GGCTAAGTCG
ACAATCAACC
TCTGGATTAC
AAAATTTGTG
AAAGATTGAC
TGGTATTCTT
AACTATGTTG
CTCCTTTTAC
GCTATGTGGA
TACGCTGCTT
TAATGCCTTT
GTATCATGCT
ATTGCTTCCC
GTATGGCTTT
CATTTTCTCC
TCCTTGTATA
AATCCTGGTT
GCTGTCTCTT
TATGAGGAGT
TGTGGCCCGT
TGTCAGGCAA
CGTGGCGTGG
TGTGCACTGT
GTTTGCTGAC
GCAACCCCCA
CTGGTTGGGG
CATTGCCACC
ACCTGTCAGC
TCCTTTCCGG
GACTTTCGCT
TTCCCCCTCC
CTATTGCCAC
GGCGGAACTC
ATCGCCGCCT
GCCTTGCCCG
CTGCTGGACA
GGGGCTCGGC
TGTTGGGCAC
TGACAATTCC
GTGGTGTTGT
CGGGGAAATC
ATCGTCCTTT
CCTTGGCTGC
TCGCCTGTGT
TGCCACCTGG
ATTCTGCGCG
GGACGTCCTT
CTGCTACGTC
CCTTCGGCCC
TCAATCCAGC
GGACCTTCCT
TCCCGCGGCC
TGCTGCCGGC
TCTGCGGCCT
CTTCCGCGTC
TTCGCCTTCG
CCCTCAGACG
AGTCGGATCT
CCCTTTGGGC
CGCCTCCCCG
CCTGGTACCT
TTAAGACCAA
TGACTTACAA
GGCAGCTGTA
GATCTTAGCC
ACTTTTTAAA
AGAAAAGGGG
GGACTGGAAG
GGCTAATTCA
CTCCCAACGA
AAATAAGATC
TGCTTTTTGC
TTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGT
AGTAGTTCAT
GTCATCTTAT
TATTCAGTAT
TTATAACTTG
CAAAGAAATG
AATATCAGAG
AGTGAGAGGA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
GCTCTAGCTA
TCCCGCCCCT
AACTCCGCCC
AGTTCCGCCC
ATTCTCCGCC
CCATGGCTGA
CTAATTTTTT
TTATTTATGC
AGAGGCCGAG
GCCGCCTCGG
CCTCTGAGCT
ATTCCAGAAG
TAGTGAGGAG
GCTTTTTTGG
AGGCCTAGAC
TTTTGCAGAG
ACGGCCCAAA
TTCGTAATCA
TGGTCATAGC
TGTTTCCTGT
GTGAAATTGT
TATCCGCTCA
CAATTCCACA
CAACATACGA
GCCGGAAGCA
TAAAGTGTAA
AGCCTGGGGT
GCCTAATGAG
TGAGCTAACT
CACATTAATT
GCGTTGCGCT
CACTGCCCGC
TTTCCAGTCG
GGAAACCTGT
CGTGCCAGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGGACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTAAATTAA
AAATGAAGTT
TTAAATCAAT
CTAAAGTATA
TATGAGTAAA
CTTGGTCTGA
CAGTTACCAA
TGCTTAATCA
GTGAGGCACC
TATCTCAGCG
ATCTGTCTAT
TTCGTTCATC
CATAGTTGCC
TGACTCCCCG
TCGTGTAGAT
AACTACGATA
CGGGAGGGCT
TACCATCTGG
CCCCAGTGCT
GCAATGATAC
CGCGAGACCC
ACGCTCACCG
GCTCCAGATT
TATCAGCAAT
AAACCAGCCA
GCCGGAAGGG
CCGAGCGCAG
AAGTGGTCCT
GCAACTTTAT
CCGCCTCCAT
CCAGTCTATT
AATTGTTGCC
GGGAAGCTAG
AGTAAGTAGT
TCGCCAGTTA
ATAGTTTGCG
CAACGTTGTT
GCCATTGCTA
CAGGCATCGT
GGTGTCACGC
TCGTCGTTTG
GTATGGCTTC
ATTCAGCTCC
GGTTCCCAAC
GATCAAGGCG
AGTTACATGA
TCCCCCATGT
TGTGCAAAAA
AGCGGTTAGC
TCCTTCGGTC
CTCCGATCGT
TGTCAGAAGT
AAGTTGGCCG
CAGTGTTATC
ACTCATGGTT
ATGGCAGCAC
TGCATAATTC
TCTTACTGTC
ATGCCATCCG
TAAGATGCTT
TTCTGTGACT
GGTGAGTACT
CAACCAAGTC
ATTCTGAGAA
TAGTGTATGC
GGCGACCGAG
TTGCTCTTGC
CCGGCGTCAA
TACGGGATAA
TACCGCGCCA
CATAGCAGAA
CTTTAAAAGT
GCTCATCATT
GGAAAACGTT
CTTCGGGGCG
AAAACTCTCA
AGGATCTTAC
CGCTGTTGAG
ATCCAGTTCG
ATGTAACCCA
CTCGTGCACC
CAACTGATCT
TCAGCATCTT
TTACTTTCAC
CAGCGTTTCT
GGGTGAGCAA
AAACAGGAAG
GCAAAATGCC
GCAAAAAAGG
GAATAAGGGC
GACACGGAAA
TGTTGAATAC
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTGACGTC
TAAGAAACCA
TTATTATCAT
GACATTAACC
TATAAAAATA
GGCGTATCAC
GAGGCCCTTT
CGTCTCGCGC
GTTTCGGTGA
TGACGGTGAA
AACCTCTGAC
ACATGCAGCT
CCCGGAGACG
GTCACAGCTT
GTCTGTAAGC
GGATGCCGGG
AGCAGACAAG
CCCGTCAGGG
CGCGTCAGCG
GGTGTTGGCG
GGTGTCGGGG
CTGGCTTAAC
TATGCGGCAT
CAGAGCAGAT
TGTACTGAGA
GTGCACCATA
TGCGGTGTGA
AATACCGCAC
AGATGCGTAA
GGAGAAAATA
CCGCATCAGG
CGCCATTCGC
CATTCAGGCT
GCGCAACTGT
TGGGAAGGGC
GATCGGTGCG
GGCCTCTTCG
CTATTACGCC
AGCTGGCGAA
AGGGGGATGT
GCTGCAAGGC
GATTAAGTTG
GGTAACGCCA
GGGTTTTCCC
AGTCACGACG
TTGTAAAACG
ACGGCCAGTG
CCAAGCTG