名称: | pACSL4(human)-Fluc-SV40-hRluc-Neo |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 启动子检测 |
片段类型: | Promoter |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | Fluc,Rluc |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CATGCAAGCT
GATCCGGCTG
CTAACAAAGC
CCGAAAGGAA
GCTGAGTTGG
CTGCTGCCAC
CGCTGAGCAA
TAACTAGCAT
AACCCCTTGG
GGCGGCCGCT
TCGAGCAGAC
ATGATAAGAT
ACATTGATGA
GTTTGGACAA
ACCACAACTA
GAATGCAGTG
AAAAAAATGC
TTTATTTGTG
AAATTTGTGA
TGCTATTGCT
TTATTTGTAA
CCATTATAAG
CTGCAATAAA
CAAGTTAACA
ACAACAATTG
CATTCATTTT
ATGTTTCAGG
TTCAGGGGGA
GATGTGGGAG
GTTTTTTTAA
GCAAGTAAAA
CCTCTACAAA
TGTGGTAAAA
TCGAATTTTA
ACAAAATATT
AACGCTTACA
ATTTCCTGAT
GCGGTATTTT
CTCCTTACGC
ATCTGTGCGG
TATTTCACAC
CGCATACGCG
GATCTGCGCA
GCACCATGGC
CTGAAATAAC
CTCTGAAAGA
GGAACTTGGT
TAGGTACCTT
CTGAGGCGGA
AAGAACCAGC
TGTGGAATGT
GTGTCAGTTA
GGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCAGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCATAGTC
CCGCCCCTAA
CTCCGCCCAT
CCCGCCCCTA
ACTCCGCCCA
GTTCCGCCCA
TTCTCCGCCC
CATGGCTGAC
TAATTTTTTT
TATTTATGCA
GAGGCCGAGG
CCGCCTCGGC
CTCTGAGCTA
TTCCAGAAGT
AGTGAGGAGG
CTTTTTTGGA
GGCCTAGGCT
TTTGCAAAAA
GCTTGATTCT
TCTGACACAA
CAGTCTCGAA
CCAAAGGCTG
GAGCCACCAT
GGCTTCCAAG
GTGTACGACC
CCGAGCAACG
CAAACGCATG
ATCACTGGGC
CTCAGTGGTG
GGCTCGCTGC
AAGCAAATGA
ACGTGCTGGA
CTCCTTCATC
AACTACTATG
ATTCCGAGAA
GCACGCCGAG
AACGCCGTGA
TTTTTCTGCA
TGGTAACGCT
GCCTCCAGCT
ACCTGTGGAG
GCACGTCGTG
CCTCACATCG
AGCCCGTGGC
TAGATGCATC
ATCCCTGATC
TGATCGGAAT
GGGTAAGTCC
GGCAAGAGCG
GGAATGGCTC
ATATCGCCTC
CTGGATCACT
ACAAGTACCT
CACCGCTTGG
TTCGAGCTGC
TGAACCTTCC
AAAGAAAATC
ATCTTTGTGG
GCCACGACTG
GGGGGCTTGT
CTGGCCTTTC
ACTACTCCTA
CGAGCACCAA
GACAAGATCA
AGGCCATCGT
CCATGCTGAG
AGTGTCGTGG
ACGTGATCGA
GTCCTGGGAC
GAGTGGCCTG
ACATCGAGGA
GGATATCGCC
CTGATCAAGA
GCGAAGAGGG
CGAGAAAATG
GTGCTTGAGA
ATAACTTCTT
CGTCGAGACC
ATGCTCCCAA
GCAAGATCAT
GCGGAAACTG
GAGCCTGAGG
AGTTCGCTGC
CTACCTGGAG
CCATTCAAGG
AGAAGGGCGA
GGTTAGACGG
CCTACCCTCT
CCTGGCCTCG
CGAGATCCCT
CTCGTTAAGG
GAGGCAAGCC
CGACGTCGTC
CAGATTGTCC
GCAACTACAA
CGCCTACCTT
CGGGCCAGCG
ACGATCTGCC
TAAGATGTTC
ATCGAGTCCG
ACCCTGGGTT
CTTTTCCAAC
GCTATTGTCG
AGGGAGCTAA
GAAGTTCCCT
AACACCGAGT
TCGTGAAGGT
GAAGGGCCTC
CACTTCAGCC
AGGAGGACGC
TCCAGATGAA
ATGGGTAAGT
ACATCAAGAG
CTTCGTGGAG
CGCGTGCTGA
AGAACGAGCA
GACCGGTGGT
GGGAGCGGAG
GTGGCGGATC
AGGTGGCGGA
GGCTCCGGAG
GGATTGAACA
AGATGGATTG
CACGCAGGTT
CTCCGGCCGC
TTGGGTGGAG
AGGCTATTCG
GCTATGACTG
GGCACAACAG
ACAATCGGCT
GCTCTGATGC
CGCCGTGTTC
CGGCTGTCAG
CGCAGGGGCG
CCCGGTTCTT
TTTGTCAAGA
CCGACCTGTC
CGGTGCCCTG
AATGAACTGC
AGGACGAGGC
AGCGCGGCTA
TCGTGGCTGG
CCACGACGGG
CGTTCCTTGC
GCAGCTGTGC
TCGACGTTGT
CACTGAAGCG
GGAAGGGACT
GGCTGCTATT
GGGCGAAGTG
CCGGGGCAGG
ATCTCCTGTC
ATCTCACCTT
GCTCCTGCCG
AGAAAGTATC
CATCATGGCT
GATGCAATGC
GGCGGCTGCA
TACGCTTGAT
CCGGCTACCT
GCCCATTCGA
CCACCAAGCG
AAACATCGCA
TCGAGCGAGC
ACGTACTCGG
ATGGAAGCCG
GTCTTGTCGA
TCAGGATGAT
CTGGACGAAG
AGCATCAGGG
GCTCGCGCCA
GCCGAACTGT
TCGCCAGGCT
CAAGGCGCGC
ATGCCCGACG
GCGAGGATCT
CGTCGTGACC
CATGGCGATG
CCTGCTTGCC
GAATATCATG
GTGGAAAATG
GCCGCTTTTC
TGGATTCATC
GACTGTGGCC
GGCTGGGTGT
GGCGGACCGC
TATCAGGACA
TAGCGTTGGC
TACCCGTGAT
ATTGCTGAAG
AGCTTGGCGG
CGAATGGGCT
GACCGCTTCC
TCGTGCTTTA
CGGTATCGCC
GCTCCCGATT
CGCAGCGCAT
CGCCTTCTAT
CGCCTTCTTG
ACGAGTTCTT
CTGAGCGGGA
CTCTGGGGTT
CGAAATGACC
GACCAAGCGA
CGCCCAACCT
GCCATCACGA
TGGCCGCAAT
AAAATATCTT
TATTTTCATT
ACATCTGTGT
GTTGGTTTTT
TGTGTGAATC
GATAGCGATA
AGGATCCTCT
TTGCGCTTGC
GTTTTCCCTT
GTCCAGATAG
CCCAGTAGCT
GACATTCATC
CGGGGTCAGC
ACCGTTTCTG
CGGACTGGCT
TTCTACGTAA
TGGTTTCTTA
GACGTCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTCAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTA
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACTATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAATTC
GAAATGACCG
ACCAAGCGAC
GCCCAACCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGGACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATTT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTATAGTCC
GGAAATACAG
GAACGCACGC
TGGATGGCCC
TTCGCTGGGA
TGGTGAAACC
ATGAAAAATG
GCAGCTTCAG
TGGATTAAGT
GGGGGTAATG
TGGCCTGTAC
CCTCTGGTTG
CATAGGTATT
CATACGGTTA
AAATTTATCA
GGCGCGATTG
CGGCAGTTTT
TCGGGTGGTT
TGTTGCCATT
TTTACCTGTC
TGCTGCCGTG
ATCGCGCTGA
ACGCGTTTTA
GCGGTGCGTA
CAATTAAGGG
ATTATGGTAA
ATCCACTTAC
TGTCTGCCCT
CGTAGCCATC
GAGATAAACC
GCAGTACTCC
GGCCACGATG
CGTCCGGCGT
AGAGGATCGA
GATCTTGGGC
AGTAATTTCT
GGAAGGTTTC
TGATGACAAG
AAATTAAGCA
AGGTTTGTTT
TGACTCCGAA
AAAGGAAAGC
TGGAAATAGC
GTGGTTATAC
TTGGTTTCCT
TTTTGGAGGT
ATAGTTCTTC
TTTCCTCTTC
AACTTGGGTG
CAGTTAAATG
TGGAAGAAGG
TGAGATGTGG
TAGGTGATGA
AGACAGCCAG
AACTGTACAC
ACTAATGAAT
AGCTTAAGTA
ATAAGAGATC
TTGAAAATCC
AAGTAGTTTC
TTGGTCTGAT
AAACATATCT
ATATGTATAT
CATACACACA
TTTGTATACC
AAAAAACGGA
TAGTTTCAGG
CTATAGTGAT
TTCTTTCCTT
TATATCTTGT
AGAGCTGGAG
GAACTTCAGT
AAGCTGAAGA
CTCTGTTCAG
AGGAAATTAC
CTAATTGAAA
ATGTCTGGGC
TAAGAGACCG
ACAATAAAGG
AAACTGGCAA
AAGTACTCTT
TAATTACAAG
TTCTTTTTCA
TCTTCATCCA
GCACACTGAT
TTATGTGCTA
CACTGGGTTT
GTATTATATC
TATATTTTAA
TTCCACAACT
AGACACTAAC
TTTATTGAGA
ACAGCGATAT
TCATTTCTTC
AATTTCATTT
GTGTTAACCC
AACTGTAGGT
GATAGTGCTC
TGTACATCAT
AGTTACCCAG
TGAATTACGT
ACCTGACATA
TGATTGCTTA
TTTCTGGTAT
TAAAACAAGA
TTTCATAAAA
TAAACATGAC
AATTAAACTC
GTTGTTTGAA
GTACTGAACT
TTGTAGGTCA
ATCTTGAAAA
AGGAAGCTAC
ATGACTGTCT
TAGCAATATT
TATAGGTATA
TTATATGTAA
GTGGGTGGGT
TTCTTGGAAA
TTGAATTGGT
AATATGGCTT
TAGAGTAGTA
ACACAAAAAC
TATAGCTCTG
TGCTCTCAGC
TAACCACTTT
TAGTGTCACC
TGGGCTGCTT
ATTAAAAATT
CAAATTTCTT
ATCCCTACCC
AGATCAGAAT
CAAAATACCT
GGGGGTTGTA
GCTCCAGGTA
CCTACATTTC
AACAAGCACC
CCAGATAATT
GTGATGCACA
CTGAAGAACT
ACTCTAGCTT
GTAATCTCAG
GTGGTAAGGC
ATTTTATATA
TACATATATA
TATACACACA
CACAAGCACA
AATGGTAATT
CTATGGGAAA
ATGGAAATCA
GCAAGTGAAG
TGAAATGTCA
ATTACCATCA
GGAACAGGAA
GCTAGTTCAC
CATTACGCAT
CAGGGAACTG
GAAGAATTCT
TGAGATTTAT
TTTTTTGGGT
GATATATTCT
TGGCAGTTTA
TCAGGCCTCA
TTTGCAATTA
TGGTCTGTCC
CTTTTGAAGC
CCTCAGATAG
TTCGCTGGCA
TTTTAATTTT
AATAGCAATT
TCTTGCATCT
GTTGGAACAG
CATTCGTATG
ACCGGCTTAT
AAGCAATGAT
CAGGGCTAGT
AAGATGTCAC
AAAGCTGCGG
TGACTTTTCC
TTATCAGTTG
GGGGTTTTTT
TGCCATGATA
CCATCGTGGA
AATGAAAGAC
AACGTGAAAA
TCCTGCGTCC
TTGTGATATT
TCATCAGTGG
GAGTGAGCGG
GGATCTTAAC
GCAGTAAGAG
GCAAGATAAA
CGGATGAGAG
GCATTAGTTC
AGGCTTACAG
GAGCAAGATG
CTTTTAGTCA
AAGTGTTCAA
GATAGCTCTC
TTAAAGAGGA
GCAGTGAAAC
CCAAAAATGA
GTGTCTTTTG
AGGGCAGCTG
GAAGATGACC
CGAGGGGAAC
TTTTATCCTG
GGGTGGAGTC
CTGCGAAGCA
AGCCGCAGGT
GAGGGCGTGG
GCCAATTCTG
CGCCTCACTG
CTGTTAGGCG
CAAGGACATC
CCGAAACACA
CTCAACGACA
CCAGCAGCGA
GACTGACAGA
AGCGGATCGC
CAGCGGGAGC
AACAACTTGA
GGCGAGCCGT
TAGCCGCCTC
GCCTTCCTCG
CAGCGCCCCT
CCCGCGCCCC
GGGAAGCCCG
TTCGGCTCCG
CCTTTTCGAC
TGACGTCCCG
GGCGAGCGGG
GGCGGGCGCG
TGGGTGGGCC
GAGCCGAATT
CTGCAGTCGA
CGGTACCGCG
GGCCCGGGAT
CCCACCATGG
AAGATGCCAA
AAACATTAAG
AAGGGCCCAG
CGCCATTCTA
CCCACTCGAA
GACGGGACCG
CCGGCGAGCA
GCTGCACAAA
GCCATGAAGC
GCTACGCCCT
GGTGCCCGGC
ACCATCGCCT
TTACCGACGC
ACATATCGAG
GTGGACATTA
CCTACGCCGA
GTACTTCGAG
ATGAGCGTTC
GGCTGGCAGA
AGCTATGAAG
CGCTATGGGC
TGAATACAAA
CCATCGGATC
GTGGTGTGCA
GCGAGAATAG
CTTGCAGTTC
TTCATGCCCG
TGTTGGGTGC
CCTGTTCATC
GGTGTGGCTG
TGGCCCCAGC
TAACGACATC
TACAACGAGC
GCGAGCTGCT
GAACAGCATG
GGCATCAGCC
AGCCCACCGT
CGTATTCGTG
AGCAAGAAAG
GGCTGCAAAA
GATCCTCAAC
GTGCAAAAGA
AGCTACCGAT
CATACAAAAG
ATCATCATCA
TGGATAGCAA
GACCGACTAC
CAGGGCTTCC
AAAGCATGTA
CACCTTCGTG
ACTTCCCATT
TGCCACCCGG
CTTCAACGAG
TACGACTTCG
TGCCCGAGAG
CTTCGACCGG
GACAAAACCA
TCGCCCTGAT
CATGAACAGT
AGTGGCAGTA
CCGGATTGCC
CAAGGGCGTA
GCCCTACCGC
ACCGCACCGC
TTGTGTCCGA
TTCAGTCATG
CCCGCGACCC
CATCTTCGGC
AACCAGATCA
TCCCCGACAC
CGCTATCCTC
AGCGTGGTGC
CATTTCACCA
CGGCTTCGGC
ATGTTCACCA
CGCTGGGCTA
CTTGATCTGC
GGCTTTCGGG
TCGTGCTCAT
GTACCGCTTC
GAGGAGGAGC
TATTCTTGCG
CAGCTTGCAA
GACTATAAGA
TTCAATCTGC
CCTGCTGGTG
CCCACACTAT
TTAGCTTCTT
CGCTAAGAGC
ACTCTCATCG
ACAAGTACGA
CCTAAGCAAC
TTGCACGAGA
TCGCCAGCGG
CGGGGCGCCG
CTCAGCAAGG
AGGTAGGTGA
GGCCGTGGCC
AAACGCTTCC
ACCTACCAGG
CATCCGCCAG
GGCTACGGCC
TGACAGAAAC
AACCAGCGCC
ATTCTGATCA
CCCCCGAAGG
GGACGACAAG
CCTGGCGCAG
TAGGCAAGGT
GGTGCCCTTC
TTCGAGGCTA
AGGTGGTGGA
CTTGGACACC
GGTAAGACAC
TGGGTGTGAA
CCAGCGCGGC
GAGCTGTGCG
TCCGTGGCCC
CATGATCATG
AGCGGCTACG
TTAACAACCC
CGAGGCTACA
AACGCTCTCA
TCGACAAGGA
CGGCTGGCTG
CACAGCGGCG
ACATCGCCTA
CTGGGACGAG
GACGAGCACT
TCTTCATCGT
GGACCGGCTG
AAGAGCCTGA
TCAAATACAA
GGGCTACCAG
GTAGCCCCAG
CCGAACTGGA
GAGCATCCTG
CTGCAACACC
CCAACATCTT
CGACGCCGGG
GTCGCCGGCC
TGCCCGACGA
CGATGCCGGC
GAGCTGCCCG
CCGCAGTCGT
CGTGCTGGAA
CACGGTAAAA
CCATGACCGA
GAAGGAGATC
GTGGACTATG
TGGCCAGCCA
GGTTACAACC
GCCAAGAAGC
TGCGCGGTGG
TGTTGTGTTC
GTGGACGAGG
TGCCTAAAGG
ACTGACCGGC
AAGTTGGACG
CCCGCAAGAT
CCGCGAGATT
CTCATTAAGG
CCAAGAAGGG
CGGCAAGATC
GCCGTGTAAT
TCTAGTTGTT
TAAACGAGCT
CGCTAGCCTC
GAGTCTAGAG
TCGACCTGCA
GG