名称: | pDHt/sk-PC |
---|---|
别称: | Plasmid#92125 |
基因长: | |
质粒宿主: | 丝状真菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Kan |
筛选标记: | Hyg |
荧光标记: | |
启动子: | |
复制子: | pVS1 |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GCGCAATTAA
CCCTCACTAA
AGGGAACAAA
AGCTGGAGCT
CCACCGCGGT
GGCGGCCGCG
ACGTTAACTG
ATATTGAAGG
AGCACTTTTT
GGGCTTGGCT
GGAGCTAGTG
GAGGTCAACA
ATGAATGCCT
ATTTTGGTTT
AGTCGTCCAG
GCGGTGAGCA
CAAAATTTGT
GTCGTTTGAC
AAGATGGTTC
ATTTAGGCAA
CTGGTCAGAT
CAGCCCCACT
TGTAGCAGTA
GCGGCGGCGC
TCGAAGTGTG
ACTCTTATTA
GCAGACAGGA
ACTAGGACAT
TATCATCATC
TGCTGCTTGG
TGCACGATAA
CTTGGTGCGT
TTGTCAAGCA
AGGTAAGTGA
ACGACCCGGT
CATACCTTCT
TAAGTTCGCC
CTTCCTCCCT
TTGTTTCAGA
TTCAATCTGA
CTTACCTATT
CTACCCAAGC
ATCGATATGA
AAAAGCCTGA
ACTCACCGCG
ACGTCTGTCG
AGAAGTTTCT
GATCGACAAG
TTCGACAGCG
TCTCCGACCT
GATGCAGCTC
TCGGAGGGCG
AAGAATCTCG
TGCTTTCAGC
TTCGATGTAG
GAGGGCGTGG
ATATGTCCTG
CGGGTAAATA
GCTGCGCCGA
TGGTTTCTAC
AAAGATCGTT
ATGTTTATCG
GCACTTTGCA
TCGGCCGCGC
TCCCGATTCC
GGAAGTGCTT
GACATTGGGG
AATTCAGCGA
GAGCCTGACC
TATTGCATCT
CCCGCCGTGC
ACAGGGTGTC
ACGTTGCAAG
ACCTGCCTGA
AACCGAACTG
CCCGCTGTTC
TGCAGCCGGT
CGCGGAGGCC
ATGGATGCGA
TCGCTGCGGC
CGATCTTAGC
CAGACGAGCG
GGTTCGGCCC
ATTCGGACCG
CAAGGAATCG
GTCAATACAC
TACATGGCGT
GATTTCATAT
GCGCGATTGC
TGATCCCCAT
GTGTATCACT
GGCAAACTGT
GATGGACGAC
ACCGTCAGTG
CGTCCGTCGC
GCAGGCTCTC
GATGAGCTGA
TGCTTTGGGC
CGAGGACTGC
CCCGAAGTCC
GGCACCTCGT
GCACGCGGAT
TTCGGCTCCA
ACAATGTCCT
GACGGACAAT
GGCCGCATAA
CAGCGGTCAT
TGACTGGAGC
GAGGCGATGT
TCGGGGATTC
CCAATACGAG
GTCGCCAACA
TCTTCTTCTG
GAGGCCGTGG
TTGGCTTGTA
TGGAGCAGCA
GACGCGCTAC
TTCGAGCGGA
GGCATCCGGA
GCTTGCAGGA
TCGCCGCGGC
TCCGGGCGTA
TATGCTCCGC
ATTGGTCTTG
ACCAACTCTA
TCAGAGCTTG
GTTGACGGCA
ATTTCGATGA
TGCAGCTTGG
GCGCAGGGTC
GATGCGACGC
AATCGTCCGA
TCCGGAGCCG
GGACTGTCGG
GCGTACACAA
ATCGCCCGCA
GAAGCGCGGC
CGTCTGGACC
GATGGCTGTG
TAGAAGTACT
CGCCGATAGT
GGAAACCGAC
GCCCCAGCAC
TCGTCCGAGG
GCAAAGGAAT
AGAGTAGATG
CCGACCGGGA
TCGATCCACT
TAACGTTACT
GAAATCATCA
AACAGCTTGA
CGAATCTGGA
TATAAGATCG
TTGGTGTCGA
TGTCAGCTCC
GGAGTTGAGA
CAAATGGTGT
TCAGGATCTC
GATAAGATAC
GTTCATTTGT
CCAAGCAGCA
AAGAGTGCCT
TCTAGTGATT
TAATAGCTCC
ATGTCAACAA
GAATAAAACG
CGTTTCGGGT
TTACCTCTTC
CAGATACAGC
TCAACTGCAA
TGCATTAATG
CATTGGACCT
CGCAACCCTA
GTACGCCCTT
CAGGCTCCGG
CGAAGCAGAA
GAATAGCTTA
GCAGAGTCTA
TTTTCATTTT
CGGGAGACGA
GATCAAGCAG
ATCAACGGTC
GTCAAGAGAC
CTACGAGACT
GAGGAATCCG
CTCTTGGCTC
CACGCGACTA
TATATTTGTC
TCTAATTGTA
CTTTGACATG
CTCCTCTTCT
TTACTCTGAT
AGCTTGACTA
TGAAAATTCC
GTCACCAGCC
CTGCTCGAGT
TTCTCCATAA
TAATGTGTGA
GTAGTTCCCA
GATAAGGGAA
TTAGGGTTCC
TATAGGGTTT
CGCTCATGTG
TTGAGCATAT
AAGAAACCCT
TAGTATGTAT
TTGTATTTGT
AAAATACTTC
TATCAATAAA
ATTTCTAATT
CCTAAAACCA
AAATCCAGTA
CTAAAATCCA
GATCCCCCGA
ATTAATTCGG
CGTTAATTCA
GTACATTAAA
AACGTCCGCA
ATGTGTTATT
AAGTTGTCTA
AGCGTCAATT
TGTTTACACC
ACAATATATC
CTGCCACCAG
CCAGCCAACA
GCTCCCCGAC
CGGCAGCTCG
GCACAAAATC
ACCACTCGAT
ACAGGCAGCC
CATCAGTCCG
GGACGGCGTC
AGCGGGAGAG
CCGTTGTAAG
GCGGCAGACT
TTGCTCATGT
TACCGATGCT
ATTCGGAAGA
ACGGCAACTA
AGCTGCCGGG
TTTGAAACAC
GGATGATCTC
GCGGAGGGTA
GCATGTTGAT
TGTAACGATG
ACAGAGCGTT
GCTGCCTGTG
ATCACCGCGG
TTTCAAAATC
GGCTCCGTCG
ATACTATGTT
ATACGCCAAC
TTTGAAAACA
ACTTTGAAAA
AGCTGTTTTC
TGGTATTTAA
GGTTTTAGAA
TGCAAGGAAC
AGTGAATTGG
AGTTCGTCTT
GTTATAATTA
GCTTCTTGGG
GTATCTTTAA
ATACTGTAGA
AAAGAGGAAG
GAAATAATAA
ATGGCTAAAA
TGAGAATATC
ACCGGAATTG
AAAAAACTGA
TCGAAAAATA
CCGCTGCGTA
AAAGATACGG
AAGGAATGTC
TCCTGCTAAG
GTATATAAGC
TGGTGGGAGA
AAATGAAAAC
CTATATTTAA
AAATGACGGA
CAGCCGGTAT
AAAGGGACCA
CCTATGATGT
GGAACGGGAA
AAGGACATGA
TGCTATGGCT
GGAAGGAAAG
CTGCCTGTTC
CAAAGGTCCT
GCACTTTGAA
CGGCATGATG
GCTGGAGCAA
TCTGCTCATG
AGTGAGGCCG
ATGGCGTCCT
TTGCTCGGAA
GAGTATGAAG
ATGAACAAAG
CCCTGAAAAG
ATTATCGAGC
TGTATGCGGA
GTGCATCAGG
CTCTTTCACT
CCATCGACAT
ATCGGATTGT
CCCTATACGA
ATAGCTTAGA
CAGCCGCTTA
GCCGAATTGG
ATTACTTACT
GAATAACGAT
CTGGCCGATG
TGGATTGCGA
AAACTGGGAA
GAAGACACTC
CATTTAAAGA
TCCGCGCGAG
CTGTATGATT
TTTTAAAGAC
GGAAAAGCCC
GAAGAGGAAC
TTGTCTTTTC
CCACGGCGAC
CTGGGAGACA
GCAACATCTT
TGTGAAAGAT
GGCAAAGTAA
GTGGCTTTAT
TGATCTTGGG
AGAAGCGGCA
GGGCGGACAA
GTGGTATGAC
ATTGCCTTCT
GCGTCCGGTC
GATCAGGGAG
GATATCGGGG
AAGAACAGTA
TGTCGAGCTA
TTTTTTGACT
TACTGGGGAT
CAAGCCTGAT
TGGGAGAAAA
TAAAATATTA
TATTTTACTG
GATGAATTGT
TTTAGTACCT
AGAATGCATG
ACCAAAATCC
CTTAACGTGA
GTTTTCGTTC
CACTGAGCGT
CAGACCCCGT
AGAAAAGATC
AAAGGATCTT
CTTGAGATCC
TTTTTTTCTG
CGCGTAATCT
GCTGCTTGCA
AACAAAAAAA
CCACCGCTAC
CAGCGGTGGT
TTGTTTGCCG
GATCAAGAGC
TACCAACTCT
TTTTCCGAAG
GTAACTGGCT
TCAGCAGAGC
GCAGATACCA
AATACTGTCC
TTCTAGTGTA
GCCGTAGTTA
GGCCACCACT
TCAAGAACTC
TGTAGCACCG
CCTACATACC
TCGCTCTGCT
AATCCTGTTA
CCAGTGGCTG
CTGCCAGTGG
CGATAAGTCG
TGTCTTACCG
GGTTGGACTC
AAGACGATAG
TTACCGGATA
AGGCGCAGCG
GTCGGGCTGA
ACGGGGGGTT
CGTGCACACA
GCCCAGCTTG
GAGCGAACGA
CCTACACCGA
ACTGAGATAC
CTACAGCGTG
AGCTATGAGA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCTT
TGAGTGAGCT
GATACCGCTC
GCCGCAGCCG
AACGACCGAG
CGCAGCGAGT
CAGTGAGCGA
GGAAGCGGAA
GAGCGCCTGA
TGCGGTATTT
TCTCCTTACG
CATCTGTGCG
GTATTTCACA
CCGCATATGG
TGCACTCTCA
GTACAATCTG
CTCTGATGCC
GCATAGTTAA
GCCAGTATAC
ACTCCGCTAT
CGCTACGTGA
CTGGGTCATG
GCTGCGCCCC
GACACCCGCC
AACACCCGCT
GACGCGCCCT
GACGGGCTTG
TCTGCTCCCG
GCATCCGCTT
ACAGACAAGC
TGTGACCGTC
TCCGGGAGCT
GCATGTGTCA
GAGGTTTTCA
CCGTCATCAC
CGAAACGCGC
GAGGCAGGGT
GCCTTGATGT
GGGCGCCGGC
GGTCGAGTGG
CGACGGCGCG
GCTTGTCCGC
GCCCTGGTAG
ATTGCCTGGC
CGTAGGCCAG
CCATTTTTGA
GCGGCCAGCG
GCCGCGATAG
GCCGACGCGA
AGCGGCGGGG
CGTAGGGAGC
GCAGCGACCG
AAGGGTAGGC
GCTTTTTGCA
GCTCTTCGGC
TGTGCGCTGG
CCAGACAGTT
ATGCACAGGC
CAGGCGGGTT
TTAAGAGTTT
TAATAAGTTT
TAAAGAGTTT
TAGGCGGAAA
AATCGCCTTT
TTTCTCTTTT
ATATCAGTCA
CTTACATGTG
TGACCGGTTC
CCAATGTACG
GCTTTGGGTT
CCCAATGTAC
GGGTTCCGGT
TCCCAATGTA
CGGCTTTGGG
TTCCCAATGT
ACGTGCTATC
CACAGGAAAG
AGACCTTTTC
GACCTTTTTC
CCCTGCTAGG
GCAATTTGCC
CTAGCATCTG
CTCCGTACAT
TAGGAACCGG
CGGATGCTTC
GCCCTCGATC
AGGTTGCGGT
AGCGCATGAC
TAGGATCGGG
CCAGCCTGCC
CCGCCTCCTC
CTTCAAATCG
TACTCCGGCA
GGTCATTTGA
CCCGATCAGC
TTGCGCACGG
TGAAACAGAA
CTTCTTGAAC
TCTCCGGCGC
TGCCACTGCG
TTCGTAGATC
GTCTTGAACA
ACCATCTGGC
TTCTGCCTTG
CCTGCGGCGC
GGCGTGCCAG
GCGGTAGAGA
AAACGGCCGA
TGCCGGGATC
GATCAAAAAG
TAATCGGGGT
GAACCGTCAG
CACGTCCGGG
TTCTTGCCTT
CTGTGATCTC
GCGGTACATC
CAATCAGCTA
GCTCGATCTC
GATGTACTCC
GGCCGCCCGG
TTTCGCTCTT
TACGATCTTG
TAGCGGCTAA
TCAAGGCTTC
ACCCTCGGAT
ACCGTCACCA
GGCGGCCGTT
CTTGGCCTTC
TTCGTACGCT
GCATGGCAAC
GTGCGTGGTG
TTTAACCGAA
TGCAGGTTTC
TACCAGGTCG
TCTTTCTGCT
TTCCGCCATC
GGCTCGCCGG
CAGAACTTGA
GTACGTCCGC
AACGTGTGGA
CGGAACACGC
GGCCGGGCTT
GTCTCCCTTC
CCTTCCCGGT
ATCGGTTCAT
GGATTCGGTT
AGATGGGAAA
CCGCCATCAG
TACCAGGTCG
TAATCCCACA
CACTGGCCAT
GCCGGCCGGC
CCTGCGGAAA
CCTCTACGTG
CCCGTCTGGA
AGCTCGTAGC
GGATCACCTC
GCCAGCTCGT
CGGTCACGCT
TCGACAGACG
GAAAACGGCC
ACGTCCATGA
TGCTGCGACT
ATCGCGGGTG
CCCACGTCAT
AGAGCATCGG
AACGAAAAAA
TCTGGTTGCT
CGTCGCCCTT
GGGCGGCTTC
CTAATCGACG
GCGCACCGGC
TGCCGGCGGT
TGCCGGGATT
CTTTGCGGAT
TCGATCAGCG
GCCGCTTGCC
ACGATTCACC
GGGGCGTGCT
TCTGCCTCGA
TGCGTTGCCG
CTGGGCGGCC
TGCGCGGCCT
TCAACTTCTC
CACCAGGTCA
TCACCCAGCG
CCGCGCCGAT
TTGTACCGGG
CCGGATGGTT
TGCGACCGCT
CACGCCGATT
CCTCGGGCTT
GGGGGTTCCA
GTGCCATTGC
AGGGCCGGCA
GGCAACCCAG
CCGCTTACGC
CTGGCCAACC
GCCCGTTCCT
CCACACATGG
GGCATTCCAC
GGCGTCGGTG
CCTGGTTGTT
CTTGATTTTC
CATGCCGCCT
CCTTTAGCCG
CTAAAATTCA
TCTACTCATT
TATTCATTTG
CTCATTTACT
CTGGTAGCTG
CGCGATGTAT
TCAGATAGCA
GCTCGGTAAT
GGTCTTGCCT
TGGCGTACCG
CGTACATCTT
CAGCTTGGTG
TGATCCTCCG
CCGGCAACTG
AAAGTTGACC
CGCTTCATGG
CTGGCGTGTC
TGCCAGGCTG
GCCAACGTTG
CAGCCTTGCT
GCTGCGTGCG
CTCGGACGGC
CGGCACTTAG
CGTGTTTGTG
CTTTTGCTCA
TTTTCTCTTT
ACCTCATTAA
CTCAAATGAG
TTTTGATTTA
ATTTCAGCGG
CCAGCGCCTG
GACCTCGCGG
GCAGCGTCGC
CCTCGGGTTC
TGATTCAAGA
ACGGTTGTGC
CGGCGGCGGC
AGTGCCTGGG
TAGCTCACGC
GCTGCGTGAT
ACGGGACTCA
AGAATGGGCA
GCTCGTACCC
GGCCAGCGCC
TCGGCAACCT
CACCGCCGAT
GCGCGTGCCT
TTGATCGCCC
GCGACACGAC
AAAGGCCGCT
TGTAGCCTTC
CATCCGTGAC
CTCAATGCGC
TGCTTAACCA
GCTCCACCAG
GTCGGCGGTG
GCCCATATGT
CGTAAGGGCT
TGGCTGCACC
GGAATCAGCA
CGAAGTCGGC
TGCCTTGATC
GCGGACACAG
CCAAGTCCGC
CGCCTGGGGC
GCTCCGTCGA
TCACTACGAA
GTCGCGCCGG
CCGATGGCCT
TCACGTCGCG
GTCAATCGTC
GGGCGGTCGA
TGCCGACAAC
GGTTAGCGGT
TGATCTTCCC
GCACGGCCGC
CCAATCGCGG
GCACTGCCCT
GGGGATCGGA
ATCGACTAAC
AGAACATCGG
CCCCGGCGAG
TTGCAGGGCG
CGGGCTAGAT
GGGTTGCGAT
GGTCGTCTTG
CCTGACCCGC
CTTTCTGGTT
AAGTACAGCG
ATAACCTTCA
TGCGTTCCCC
TTGCGTATTT
GTTTATTTAC
TCATCGCATC
ATATACGCAG
CGACCGCATG
ACGCAAGCTG
TTTTACTCAA
ATACACATCA
CCTTTTTAGA
CGGCGGCGCT
CGGTTTCTTC
AGCGGCCAAG
CTGGCCGGCC
AGGCCGCCAG
CTTGGCATCA
GACAAACCGG
CCAGGATTTC
ATGCAGCCGC
ACGGTTGAGA
CGTGCGCGGG
CGGCTCGAAC
ACGTACCCGG
CCGCGATCAT
CTCCGCCTCG
ATCTCTTCGG
TAATGAAAAA
CGGTTCGTCC
TGGCCGTCCT
GGTGCGGTTT
CATGCTTGTT
CCTCTTGGCG
TTCATTCTCG
GCGGCCGCCA
GGGCGTCGGC
CTCGGTCAAT
GCGTCCTCAC
GGAAGGCACC
GCGCCGCCTG
GCCTCGGTGG
GCGTCACTTC
CTCGCTGCGC
TCAAGTGCGC
GGTACAGGGT
CGAGCGATGC
ACGCCAAGCA
GTGCAGCCGC
CTCTTTCACG
GTGCGGCCTT
CCTGGTCGAT
CAGCTCGCGG
GCGTGCGCGA
TCTGTGCCGG
GGTGAGGGTA
GGGCGGGGGC
CAAACTTCAC
GCCTCGGGCC
TTGGCGGCCT
CGCGCCCGCT
CCGGGTGCGG
TCGATGATTA
GGGAACGCTC
GAACTCGGCA
ATGCCGGCGA
ACACGGTCAA
CACCATGCGG
CCGGCCGGCG
TGGTGGTGTC
GGCCCACGGC
TCTGCCAGGC
TACGCAGGCC
CGCGCCGGCC
TCCTGGATGC
GCTCGGCAAT
GTCCAGTAGG
TCGCGGGTGC
TGCGGGCCAG
GCGGTCTAGC
CTGGTCACTG
TCACAACGTC
GCCAGGGCGT
AGGTGGTCAA
GCATCCTGGC
CAGCTCCGGG
CGGTCGCGCC
TGGTGCCGGT
GATCTTCTCG
GAAAACAGCT
TGGTGCAGCC
GGCCGCGTGC
AGTTCGGCCC
GTTGGTTGGT
CAAGTCCTGG
TCGTCGGTGC
TGACGCGGGC
ATAGCCCAGC
AGGCCAGCGG
CGGCGCTCTT
GTTCATGGCG
TAATGTCTCC
GGTTCTAGTC
GCAAGTATTC
TACTTTATGC
GACTAAAACA
CGCGACAAGA
AAACGCCAGG
AAAAGGGCAG
GGCGGCAGCC
TGTCGCGTAA
CTTAGGACTT
GTGCGACATG
TCGTTTTCAG
AAGACGGCTG
CACTGAACGT
CAGAAGCCGA
CTGCACTATA
GCAGCGGAGG
GGTTGGATCA
AAGTACTTTG
ATCCCGAGGG
GAACCCTGTG
GTTGGCATGC
ACATACAAAT
GGACGAACGG
ATAAACCTTT
TCACGCCCTT
TTAAATATCC
GATTATTCTA
ATAAACGCTC
TTTTCTCTTA
GGTTTACCCG
CCAATATATC
CTGTCAAACA
CTGATAGTTT
AAACTGAAGG
CGGGAAACGA
CAATCTGATC
CAAGCTCAAG
CTGCTCTAGC
ATTCGCCATT
CAGGCTGCGC
AACTGTTGGG
AAGGGCGATC
GGTGCGGGCC
TCTTCGCTAT
TACGCCAGCT
GGCGAAAGGG
GGATGTGCTG
CAAGGCGATT
AAGTTGGGTA
ACGCCAGGGT
TTTCCCAGTC
ACGACGTTGT
AAAACGACGG
CCAGTGCCAA
GCTTAGGACT
TCCAGGGCTA
CTTGGCGCGG
CAGAAGCCGC
AGGTGTCGAG
CCGGGAGGAG
TTTTCGCAGT
GGCTGTGCCG
CGCGCACAAC
GATGTCAACC
GGAAGCTGGG
CAAGCCCGAG
TTTGACTGCT
CGCGGGTGGA
CGAGCGGTGG
AGGACGGGGT
GGAAGGACGG
TCGGTGCGAC
TGATTGGCCA
TGAATTCCAT
CTTTCGAGGA
CGGACGAAGA
TACTGTACGA
TTAATGAAAG
GAGGGAGCAT
TCTTCGACTT
GCGGCAATTG
CATGCACATG
TACGATTGGA
AGCGCGGGCG
ATGTATTCGC
AATCATGTTT
AGAAGGACGG
CGTTTGGAAA
CGTTGGGATG
CTGTTGAAGC
GTTGGAAACA
GGGGCAATTA
GAAACACCGA
GCCAGACAGA
GTCAATGGTA
CGAGGTCAGC
CAGTATCATG
ACCTGTGTGC
GCATGGTGGC
GAGAGATTCC
GAGCCATGCC
ACGGGAGACG
AGCAATGAAA
AAACTCTTCA
CTCACTTGTC
GAGGCTCTCT
CAACCTATCG
ACTTATCAAG
TAGACGATGA
AAGCCTTGCA
ACTGTGGTGA
TGTGGCTCAT
CAATGTGCGA
CGTCGTATCC
ATGTCTGAGG
CCATTCGATA
TCGTGATGCG
ACTACCTAGT
AAAGCCCGGC
CAGAGGGCAA
ACCGGGGCGA
CAGGGGCAGG
CAATTGACCG
GATGGCTGCA
TGTGCCGAAG
CAGCCCCGAT
GGAATCGAGA
TGTCTGTCGG
ATGGACCGCT
GAGCGGCCTG
GCAAGGTGTC
CCAGATACGA
AGATGGAAGT
GAAGTCAGAG
GTGGTCGTTA
ATTGTCCGAC
GAGCGAATCG
GCCGCTCCTT
CGGATTGCCG
GCTCTGCTGT
ATGTACCGTG
CATGAAGCCA
CCCGGGATCC
ATGTTACGAT
GGATAGGTTC
CAACTCTCTA
GTAGCTATAG
TGGACCTGAG
GCTATCTAGT
ATCACTGGAG
GAGCAGCCGT
CCACTATCGT
CGAGCGCTGT
AGAAGCAGCT
GCATTAGCGG
CTGCCCACCC
GCGCAGAAAT
GGCCCCATTA
CATCACTATC
ATGACAGCGG
CGCGTCCAAA
AGTGAGCTCA
TGCTTGCCGA
TGGCACGAGC
AGCTGCAACT
GGCGGGGCTC
CTGCCTGCCG
TCTCCGGTGC
CGCTGCCCAT
TTGAGTTTGT
CCGAGCTGTT
GATGGTTGAA
ACCGAGACCG
ATGGATGATT
CAACACTTCG
AAGTCTAGGT
AGATAAAAAA
CATCTATATA
TCCTCATTCA
TTGCCCTGTC
AGTGTGTTGG
CTCACGTCTC
CAATCCTCCG
CCCCTCCTCC
TGCAAAGTAA
ATACCTTCTC
AAAACACGTC
TGGAATCCTG
CAAGTCTCCA
TCACAAGGAG
CTTCTTCATC
AACCACCTTA
TACGAGCAAC
ATCATTTGCA
TCATCGTTGA
TCCACATCTC
CTCGCGCCTC
AGAGTGTCGT
CACCAGTATA
AATAACCGCA
TCAAGCTCTC
GTCCTTCTTC
GTTCCACAAT
CCAAGAAGCA
CCTCAAAACG
ATCAAAGCAG
CGCAGCTACA
GCACAATCAT
GGACAAGAAG
TACAGCATTG
GCCTGGACAT
TGGCACGAAC
TCGGTCGGCT
GGGCCGTCAT
CACGGACGAG
TACAAGGTCC
CCTCCAAGAA
GTTTAAGGTC
CTGGGCAACA
CCGACCGCCA
CTCCATCAAG
AAGAACCTCA
TTGGCGCCCT
GCTCTTCGAC
TCCGGCGAGA
CCGCCGAGGC
CACCCGCCTC
AAGCGCACCG
CCCGCCGCCG
ATACACGCGC
CGCAAGAACC
GCATCTGCTA
CCTGCAGGAG
ATTTTCTCCA
ACGAGATGGC
CAAGGTCGAC
GACTCCTTCT
TTCACCGCCT
GGAGGAGTCG
TTCCTCGTCG
AGGAAGACAA
GAAGCACGAG
CGCCACCCCA
TCTTTGGCAA
CATTGTCGAC
GAGGTCGCCT
ACCACGAGAA
GTACCCCACG
ATCTACCACC
TGCGCAAGAA
GCTCGTCGAC
TCCACCGACA
AGGCCGACCT
CCGCCTGATC
TACCTCGCCC
TGGCCCACAT
GATTAAGTTC
CGCGGCCACT
TTCTGATCGA
GGGCGACCTC
AACCCCGACA
ACAGCGACGT
CGACAAGCTG
TTCATCCAGC
TCGTCCAGAC
CTACAACCAG
CTCTTTGAGG
AGAACCCCAT
TAACGCCTCC
GGCGTCGACG
CCAAGGCCAT
CCTCTCGGCC
CGCCTCTCCA
AGAGCCGCCG
ACTCGAGAAC
CTGATCGCCC
AGCTGCCCGG
CGAGAAGAAG
AACGGCCTGT
TCGGCAACCT
CATCGCCCTC
TCCCTGGGCC
TCACCCCCAA
CTTCAAGTCG
AACTTTGACC
TCGCCGAGGA
CGCCAAGCTG
CAGCTCTCCA
AGGACACCTA
CGACGACGAC
CTGGACAACC
TCCTGGCCCA
GATCGGCGAC
CAGTACGCCG
ACCTGTTCCT
CGCCGCCAAG
AACCTGTCCG
ACGCCATCCT
CCTGTCGGAC
ATTCTCCGCG
TCAACACCGA
GATTACGAAG
GCCCCTCTCT
CCGCCTCGAT
GATCAAGCGC
TACGACGAGC
ACCACCAGGA
CCTGACCCTG
CTCAAGGCCC
TGGTCCGCCA
GCAGCTCCCC
GAGAAGTACA
AGGAGATCTT
CTTTGACCAG
AGCAAGAACG
GCTACGCCGG
CTACATCGAC
GGCGGCGCTA
GCCAAGAGGA
GTTCTACAAG
TTTATCAAGC
CCATTCTGGA
GAAGATGGAC
GGCACGGAGG
AGCTCCTGGT
CAAGCTCAAC
CGCGAGGACC
TCCTGCGCAA
GCAGCGCACC
TTCGACAACG
GCAGCATCCC
CCACCAGATT
CACCTCGGCG
AGCTGCACGC
CATCCTCCGC
CGACAAGAGG
ACTTCTACCC
CTTTCTCAAG
GACAACCGCG
AGAAGATCGA
GAAGATTCTG
ACGTTCCGCA
TCCCCTACTA
CGTCGGCCCC
CTGGCCCGCG
GCAACAGCCG
CTTTGCCTGG
ATGACCCGCA
AGTCCGAGGA
GACCATCACG
CCCTGGAACT
TCGAGGAAGT
CGTCGACAAG
GGCGCCTCGG
CCCAGTCCTT
CATCGAGCGC
ATGACCAACT
TTGACAAGAA
CCTGCCCAAC
GAGAAGGTCC
TCCCCAAGCA
CTCGCTCCTG
TACGAGTACT
TCACCGTCTA
CAACGAGCTC
ACGAAGGTCA
AGTACGTCAC
CGAGGGCATG
CGCAAGCCCG
CCTTCCTGTC
GGGCGAGCAG
AAGAAGGCCA
TCGTCGACCT
CCTGTTTAAG
ACCAACCGCA
AGGTCACGGT
CAAGCAGCTC
AAGGAAGACT
ACTTCAAGAA
GATTGAGTGC
TTTGACAGCG
TCGAGATCTC
CGGCGTCGAG
GACCGCTTTA
ACGCCTCCCT
GGGCACCTAC
CACGACCTCC
TGAAGATCAT
TAAGGACAAG
GACTTCCTGG
ACAACGAGGA
GAACGAGGAC
ATCCTCGAGG
ACATTGTCCT
GACCCTCACG
CTGTTTGAGG
ACCGCGAGAT
GATCGAGGAG
CGCCTGAAGA
CGTACGCCCA
CCTCTTCGAC
GACAAGGTCA
TGAAGCAGCT
CAAGCGCCGC
CGATACACCG
GCTGGGGCCG
CCTGAGCCGC
AAGCTCATCA
ACGGCATTCG
CGACAAGCAG
TCGGGCAAGA
CGATCCTCGA
CTTCCTGAAG
AGCGACGGCT
TCGCCAACCG
CAACTTTATG
CAGCTGATTC
ACGACGACTC
CCTCACCTTC
AAGGAAGACA
TCCAGAAGGC
CCAGGTCTCC
GGCCAGGGCG
ACTCCCTGCA
CGAGCACATC
GCCAACCTCG
CCGGCAGCCC
CGCCATCAAG
AAGGGCATTC
TGCAGACCGT
CAAGGTCGTC
GACGAGCTCG
TCAAGGTCAT
GGGCCGCCAC
AAGCCCGAGA
ACATCGTCAT
TGAGATGGCC
CGCGAGAACC
AGACCACGCA
GAAGGGCCAG
AAGAACAGCC
GCGAGCGCAT
GAAGCGCATC
GAGGAAGGCA
TCAAGGAGCT
GGGCTCCCAG
ATCCTCAAGG
AGCACCCCGT
CGAGAACACC
CAGCTGCAGA
ACGAGAAGCT
CTACCTGTAC
TACCTCCAGA
ACGGCCGCGA
CATGTACGTC
GACCAGGAGC
TGGACATTAA
CCGCCTCTCG
GACTACGACG
TCGACCACAT
CGTCCCCCAG
AGCTTCCTGA
AGGACGACTC
CATCGACAAC
AAGGTCCTCA
CCCGCAGCGA
CAAGAACCGC
GGCAAGAGCG
ACAACGTCCC
CTCCGAGGAA
GTCGTCAAGA
AGATGAAGAA
CTACTGGCGC
CAGCTCCTGA
ACGCCAAGCT
GATCACGCAG
CGCAAGTTTG
ACAACCTCAC
CAAGGCCGAG
CGAGGCGGCC
TCTCGGAGCT
GGACAAGGCC
GGCTTCATCA
AGCGCCAGCT
GGTCGAGACC
CGCCAGATCA
CGAAGCACGT
CGCCCAGATT
CTCGACTCGC
GCATGAACAC
GAAGTACGAC
GAGAACGACA
AGCTGATCCG
CGAGGTCAAG
GTCATTACCC
TGAAGTCGAA
GCTCGTCAGC
GACTTCCGCA
AGGACTTCCA
GTTTTACAAG
GTCCGCGAGA
TCAACAACTA
CCACCACGCC
CACGACGCCT
ACCTCAACGC
CGTCGTCGGC
ACCGCCCTGA
TCAAGAAGTA
CCCCAAGCTC
GAGTCCGAGT
TCGTCTACGG
CGACTACAAG
GTCTACGACG
TCCGCAAGAT
GATCGCCAAG
TCCGAGCAGG
AGATTGGCAA
GGCCACCGCC
AAGTACTTCT
TTTACTCGAA
CATCATGAAC
TTCTTTAAGA
CCGAGATCAC
CCTCGCCAAC
GGCGAGATCC
GCAAGCGCCC
CCTCATTGAG
ACCAACGGCG
AGACCGGCGA
GATCGTCTGG
GACAAGGGCC
GCGACTTCGC
CACCGTCCGC
AAGGTCCTCA
GCATGCCCCA
GGTCAACATC
GTCAAGAAGA
CCGAGGTCCA
GACGGGCGGC
TTCTCGAAGG
AGAGCATTCT
GCCCAAGCGC
AACTCCGACA
AGCTCATCGC
CCGCAAGAAG
GACTGGGACC
CCAAGAAGTA
CGGTGGCTTC
GACTCCCCCA
CCGTCGCCTA
CTCGGTCCTG
GTCGTCGCCA
AGGTCGAGAA
GGGCAAGTCG
AAGAAGCTCA
AGAGCGTCAA
GGAGCTCCTG
GGCATCACCA
TTATGGAGCG
CAGCTCCTTC
GAGAAGAACC
CCATCGACTT
TCTCGAGGCC
AAGGGCTACA
AGGAAGTCAA
GAAGGACCTG
ATCATTAAGC
TCCCCAAGTA
CTCCCTCTTC
GAGCTGGAGA
ACGGCCGCAA
GCGCATGCTC
GCCTCCGCCG
GCGAGCTCCA
GAAGGGCAAC
GAGCTCGCCC
TGCCCAGCAA
GTACGTCAAC
TTCCTCTACC
TGGCCAGCCA
CTACGAGAAG
CTCAAGGGCT
CCCCCGAGGA
CAACGAGCAG
AAGCAGCTGT
TTGTCGAGCA
GCACAAGCAC
TACCTCGACG
AGATCATTGA
GCAGATTTCC
GAGTTCTCGA
AGCGCGTCAT
CCTGGCCGAC
GCCAACCTGG
ACAAGGTCCT
CAGCGCCTAC
AACAAGCACC
GCGACAAGCC
CATCCGCGAG
CAGGCCGAGA
ACATCATTCA
CCTCTTCACC
CTGACCAACC
TCGGCGCCCC
CGCCGCCTTC
AAGTACTTTG
ACACCACGAT
CGACCGCAAG
CGCTACACCT
CGACGAAGGA
AGTCCTGGAC
GCCACCCTCA
TCCACCAGAG
CATTACCGGC
CTCTACGAGA
CGCGCATCGA
CCTCAGCCAG
CTCGGCGGCG
ACTCCCGCGC
CGACCCCAAG
AAGAAGCGCA
AGGTCTAACC
CGGCATGAAG
TCTGACCGGG
TAGTATGAGG
GTTCATCGTC
ACCTTGATAG
AATAATAGAC
GATAAAGCAG
GCCACGGGCA
GGTACCGATT
GTCAATCCGG
CAGGTTCGGA
GGCGTGTTGG
AAATGAGTTT
ATGGGTTATG
GTCAAATCGG
ATAGTATGAG
GTACATAGTT
TGTAAATCTC
AAGATTATTT
TCTTCCTTAA
TCTTGCACGT
CGCATGAGAG
GGACCGAGAA
GAGAATTGAT
GAAGGGCTCT
TGAAGATGAG
ATGAATCACG
TGGTTGCTGA
AGCTTCAGTA
GTCTCGGGTA
CCTGTTCTTT
CCCACAAACA
GTAGCCAGGC
TAGAGGTACT
GAGTACCCGC
TCACCGTATC
TAATCATCCG
ACCTGAAATC
TTCAAGCTGT
TTTATTGACA
CTTCGAGTCC
ATCTTCATTC
ACGTAAGGAG
AACTTCTAGG
ACATCACTTA
TCCCGCCATA
TTTAGCTGCA
AGGAGTCAAT
TGCAATGTCA
GATTCCGCTC
CTAAGAGGAA
ACAGGGCCCT
GGCGGCTCAG
ATGGCTCGGC
ATTGAAGAAG
AGAAAGGTAT
GATGACAAGA
ATGCTTCTTT
TACATATTTA
CGAAGAATGC
CACCACCCTT
CTCTCTGGCT
ACCTATGTCC
ATGGACAACG
AGCTTGCTGA
CAATGGGACT
ATGAATGGGT
ACCTAGGAGG
GCACGGGAGG
AAGATGGAAG
ACGCCAGCTC
AAAAGGCAAA
GCAGGCCTCG
TGGGTCGAGA
TGGGCAAGGC
TCTCGAGGTG
GGCGACGAAG
GCATCTGGGT
CACGTATGCG
AGAGGCATGA
AGAACAAGGC
CGTTCGGGAG
TTTACGGCCA
TGTGCCATGA
GGTAAAGCTG
CATAATCCCA
TGCCCACCCA
CGGGGAGACT
ATTTTAGATG
TTTATTGGTT
GCGGCGGGGA
TGATAATAAC
GATGTTGGCA
GTACGGCGAA
AAGCTCTACG
GGATCACGCC
CCCGGCCGAC
GAGTCAGAAG
CTCACCACCC
GGCCGACGGA
GCCGAAGAGG
AAGACATCGA
GGCGTCCATC
TAGAGGATCC