名称: | pAC1430 |
---|---|
别称: | Plasmid#127981 |
基因长: | |
质粒宿主: | 丝状真菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | pyrG |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CATACGTAGC
AAGATAGACC
GCAAGAAAAT
GATGCTCGGA
ATCCATATTG
ACTCACGAAA
ATTTCTTTCT
TTTTGCCCCC
CTCATCTGTT
GCCTGGGAGG
TGAGTCAAAT
TGCCCCTCAC
TAAACGCTCC
TTTGCTTTTC
CCGAACTTGG
CCCGTTCGAG
AGCATGATCA
GCACAAAGGC
TTAACTTGGG
CGGTGATTCT
GCTGTCTCGG
CTGAGGTCTT
AATTAAACCC
TCAGCATTAA
ACCCTCAGCG
GAAACAGCTA
TGACCATGAG
ATCTTCCCGG
GCCCAGTATA
TGTTCCGCAG
ATGACTGGAG
CTCTGCCATA
CGTGCCCTCT
CAAGCACCAT
TTGTTCCATC
TACAGAGACT
AGTCACCAAC
TAGTCTATCA
AGACTCACAG
GGTACATTGC
TGAGACCAAC
TGACCAGAGG
CAGGGTAGCG
GATTGACGGC
TCCATCTCCT
TCACTTACAA
GGTCTATTGA
AAGCCCTTTA
GCATCACCAA
GCGGAGAATA
GATTGTTAAG
CTTATTTTTT
GTATACTGTT
TTGTGATAGC
ACGAAGTTTT
TCCACGGTAT
CTTGTTAAAA
ATATATATTT
GTGGCGGGCT
TACCTACATC
AAATTAATAA
GAGACTAATT
ATAAACTAAA
CACACAAGCA
AGCTACTTTA
GGGTAAAAGT
TTATAAATGC
TTTTGACGTA
TAAACGTTGC
TTGTATTTAT
TATTACAATT
AAAGGTGGAT
AGAAAACCTA
GAGACTAGTT
AGAAACTAAT
CTCAGGTTTG
CGTTAAACTA
AATCAGAGCC
CGAGAGGTTA
ACAGAACCTA
GAAGGGGACT
AGATATCCGG
GTAGGGAAAC
nAAAAAAAAA
AAACAAGACA
GCCACATATT
AGGGAGACTA
GTTAGAAGCT
AGTTCCAGGA
CTAGGAAAAT
AAAAGACAAT
GATACCACAG
TCTAGTTGAC
AACTAGATAG
ATTCTAGATT
GAGGCCAAAG
TCTCTGAGAT
CCAGGTTAGT
TGCAACTAAT
ACTAGTTAGT
ATCTAGTCTC
CTATAACTCT
GAAGCTAGAA
TAACTTACTA
CTATTATCCT
CACCACTGTT
CAGCTGCGCA
AACGGAGTGA
TTGCAAGGTG
TTCAGAGACT
AGTTATTGAC
TAGTCAGTGA
CTAGCAATAA
CTAACAAGGT
ATTAACCTAC
CATGTCTGCC
ATCACCCTGC
ACTTCCTCGG
GCTCAGCAGC
CTTTTCCTCC
TCATTTTCAT
GCTCATTTTC
CTTGTTTAAG
ACTGTGACTA
GTCAAAGACT
AGTCCAGAAC
CACAAAGGAG
AAATGTCTTA
CCACTTTCTT
CATTGCTTGT
CTCTTTTGCA
TTATCCATGT
CTGCAACTAG
TTAGAGTCTA
GTTAGTGACT
AGTCCGACGA
GGACTTGCTT
GTCTCCGGAT
TGTTGGAGGA
ACTCTCCAGG
GCCTCAAGAT
CCACAACAGA
GCCTTCTAGA
AGACTGGTCA
ATAACTAGTT
GGTCTTTGTC
TGAGTCTGAC
TTACGAGGTT
GCATACTCGC
TCCCTTTGCC
TCGTCAATCG
ATGAGAAAAA
GCGCCAAAAC
TCGCAATATG
GCTTTGAACC
ACACGGTGCT
GAGACTAGTT
AGAATCTAGT
CCCAAACTAG
CTTGGATAGC
TTACCTTTGC
CCTTTGCGTT
GCGACAGGTC
TTGCAGGGTA
TGGTTCCTTT
CTCACCAGCT
GATTTAGCTG
CCTTGCTACm
CTCACGGCGG
ATCTGCATAA
AGAGTGGCTA
GAGGTTATAA
ATTAGCACTG
ATCCTAGGTA
CGGGGCTGAA
TGTAACTTGC
CyTTCCTTTC
TCATCGCGCG
GCAAGACAGG
CTTGCTCAAA
TTCCTACCAG
TCACAGGGGT
ATGCACGGCG
TACGGACCAC
TTGAACTAGT
CACAGATTAG
TTAGCAACTA
GTCTGCATTG
AATGGCTGTA
CTTACGGGCC
CTCGCCATTG
TCCTGATCAT
TTCCAGCTTC
ACCCTCGTTG
CTGCAAAGTA
GTTAGTGACT
AGTCAAGGAC
TAGTTGAAAT
GGGAGAAGAA
ACTCACGAAT
TCTCGACACC
CTTAGTATTG
TGGTCCTTGG
ACTTGGTGCT
GCTATATATT
AGCTAATACA
CTAGTTAGAC
TCACAGAAAC
TTACGCAGCT
CGCTTGCGCT
TCTTGGTAGG
AGTCGGGGTT
GGGAGAACAG
TGCCTTCAAA
CAAGCCTTCA
TACCATGCTA
CTTGACTAGT
CAGGGACTAG
TCACCAAGTA
ATCTAGATAG
GACTTkCCTT
TGGCCTCCAT
CAGTTCCTTC
ATAGTGGGAG
GTCCATTGTG
CAATGTAAAC
TCCATGCCGT
GGGAGTTCTT
GTCCTTCAAG
TGCTTGACCA
ATATGTTTCT
GTTGGCAGAG
GGAACCTGTC
AACTAGTTAA
TAACTAGTCA
GAAACTAGTA
TAGCAGTAGA
CTCACTGTAC
GCTTGAGGCA
TCCCTTCACT
CGGCAGTAGA
CTTCATATGG
ATGGATATCA
GGCACGCCAT
TGTCGTCCTG
TGGACTAGTC
AGTAACTAGG
CTTAAAGCTA
GTCGGGTCGG
CTTACTATCT
TGAAATCCGG
CAGCGTAAGC
TCCCCGTCCT
TAACTGCCTC
GAGATAGTGA
CAGTACTCTG
GGGACTTTCG
GAGATCGTTA
TCGCGAATGC
TCGGCATACT
AATCGTTGAC
TAGTCTTGGA
CTAGTCCCGA
GCAAAAAGGA
TTGGAGGAGG
AGGAGGAAGG
TGAGAGTGAG
ACAAAGAGCG
AAATAAGAGC
TTCAAAGGCT
ATCTCTAAGC
AGTATGAAGG
TTAAGTATCT
AGTTCTTGAC
TAGATTTAAA
AGAGATTTCG
ACTAGTTATG
TACCTGGAGT
TTGGATATAG
GAATGTGTTG
TGGTAACGAA
ATGTAAGGGG
GAGGAAAGAA
AAAGTCGGTC
AAGAGGTAAC
TCTAAGTCGG
CCATTCCTTT
TTGGGAGGCG
CTAACCATAA
ACGGCATGGT
CGACTTAGAG
TTAGCTCAGG
GAATTTAGGG
AGTTATCTGC
GACCACTGGA
CTCTCACCGT
TTCCATTGGG
GTACTAACAT
AGCCATCAAA
TGCCGGCCCG
TGTTTAATCT
CCTCGAGATG
GCTGGGCTCA
CATGCGTTCT
ACTCAAAAAC
TCTTATACCT
AGGCCCTCTC
CCAATCGTGT
ATGTTTGGAC
CTTCCTCTCT
CACACTTTGG
ATAACATCAA
GCATACTGCA
GATTTTGACC
TAGTTAACAA
GGCACATCAT
GCTATCACTT
CCAACACTCT
TGCTGAGCGC
GTTCTGCCTT
GCCTTTCTGG
TTCTACGAAG
TATCCAGTCC
CGCCTGCAAC
GCCGCAGAAA
CGCCAAAAGG
TGGGGTTGTC
AACCGCCAGC
TAGAGCTCCA
TCGGGATTCT
TTGGCATTCA
GCCGTTCCTC
GGTGGTCGCA
GATAACTCCC
TAAATTCCCT
GAGCTAACTC
TAAGTCGACC
ATGCCGTTTA
TGGTTAGCGC
CTCCCAAAAA
GGAATGGCCG
ACTTAGAGTT
ACCTCTTGAC
CGACTTTTTC
TTTCCTCCCC
CTTACATTTC
GTTACCACAA
CACATTCCTA
TATCCAAACT
CCAGGTACAT
AACTAGTCGA
AATCTCTTTT
AAATCTAGTC
AAGAACTAGA
TACTTAACCT
TCATACTGCT
TAGAGATAGC
CTTTGAAGCT
CTTATTTCGC
TCTTTGTCTC
ACTCTCACCT
TCCTCCTCCT
CCTCCAATCC
TTTTTGCTCG
GGACTAGTCC
AAGACTAGTC
AACGATTAGT
ATGCCGAGCA
TTCGCGATAA
CGATCTCCGA
AAGTCCCCAG
AGTACTGTCA
CTATCTCGAG
GCAGTTAAGG
ACGGGGAGCT
TACGCTGCCG
GATTTCAAGA
TAGTAAGCCG
ACCCGACTAG
CTTTAAGCCT
AGTTACTGAC
TAGTCCACAG
GACGACAATG
GCGTGCCTGA
TATCCATCmA
TATGAAGTCT
ACTGCCGAGT
GAAGGGATGC
CTCAAGCGTA
CAGTGAGTCT
ACTGCTATAC
TAGTTTCTGA
CTAGTTATTA
ACTAGTTGAC
AGGTTCCCTC
TGCCAACAGA
AACATATTGG
TCAAGCACTT
GAAGGACAAG
AACTCCCACG
GCATGGAGTT
TACATTGCAC
AATGGACCTC
CCACTATGAA
GGAACTGATG
GAGGCCAAAG
GmAAGTCCTA
TCTAGATTAC
TTGGTGACTA
GTCCCTGACT
AGTCAAGTAG
CATGGTATGA
AGGCTTGTTT
GAAGGCACTG
TTCTCCCAAC
CCCGACTCCT
ACCAAGAAGC
GCAAGCGAGC
TGCGTAAGTT
TCTGTGAGTC
TAACTAGTGT
ATTAGCTAAT
ATATAGCAGC
ACCAAGTCCA
AGGACCACAA
TACTAAGGGT
GTCGAGAATT
CGTGAGTTTC
TTCTCCCATT
TCAACTAGTC
CTTGACTAGT
CACTAACTAC
TTTGCAGCAA
CGAGGGTGAA
GCTGGAAATG
ATCAGGACAA
TGGCGAGGGC
CCGTAAGTAC
AGCCATTCAA
TGCAGACTAG
TTGCTAACTA
ATCTGTGACT
AGTTCAAGTG
GTCCGTACGC
CGTGCATACC
CCTGTGACTG
GTAGGAATTT
GAGCAAGCCT
GTCTTGCCGC
GCGATGAGAA
AGGAArGGCA
AGTTACATTC
AGCCCCGTAC
CTAGGATCAG
TGCTAATTTA
TAACCTCTAG
CCACTCTTTA
TGCAGATCCG
CCGTGAGkGT
AGCAAGGCAG
CTAAATCAGC
TGGTGAGAAA
GGAACCATAC
CCTGCAAGAC
CTGTCGCAAC
GCAAAGGGCA
AAGGTAAGCT
ATCCAAGCTA
GTTTGGGACT
AGATTCTAAC
TAGTCTCAGC
ACCGTGTGGT
TCAAAGCCAT
ATTGCGAGTT
TTGGCGCTTT
TTCTCATCGA
TTGACGAGGC
AAAGGGAGCG
AGTATGCAAC
CTCGTAAGTC
AGACTCAGAC
AAAGACCAAC
TAGTTATTGA
CCAGTCTTCT
AGAAGGCTCT
GTTGTGGATC
TTGAGGCCCT
GGAGAGTTCC
TCCAACAATC
CGGAGACAAG
CAAGTCCTCG
TCGGACTAGT
CACTAACTAG
ACTCTAACTA
GTTGCAGACA
TGGATAATGC
AAAAGAGACA
AGCAATGAAG
AAAGTGGTAA
GACATTTCTC
CTTTGTGGTT
CTGGACTAGT
CTTTGACTAG
TCACAGTCTT
AAACAAGGAA
AATGAGCATG
AAAATGAGGA
GGAAAAGGCT
GCTGAGCCCG
AGGAAGTGCA
GGGTGATGGC
AGACATGGTA
GGTTAATACC
TTGTTAGTTA
TTGCTAGTCA
CTGACTAGTC
AATAACTAGT
CTCTGAACAC
CTTGCAATCA
CTCCGTTTGC
GCAGCTGAAC
AGTGGTGAGG
ATAATAGTAG
TAAGTTATTC
TAGCTTCAGA
GTTATAGGAG
ACTAGATACT
AACTAGTATT
AGTTGCAACT
AACCTGGATC
TCAGAGACTT
TGGCCTCAAT
CTAGAATCTA
TCTAGTTGTC
AACTAGACTG
TGGTATCATT
GTCTTTTATT
TTCCTAGTCC
TGGAACTAGC
TTCTAACTAG
TCTCCCTAAT
ATGTGGCTGT
CTTGTTTTTT
TTTTTTTGTT
TCCCTACCCG
GATATCTAGT
CCCCTTCTAG
GTTCTGTTAA
CCTCTCGGGC
TCTGATTTAG
TTTAACGCAA
ACCTGAGATT
AGTTTCTAAC
TAGTCTCTAG
GTTTTCTATC
CACCTTTAAT
TGTAATAATA
AATACAAGCA
ACGTTTATAC
GTCAAAAGCA
TTTATAAACT
TTTACCCTAA
AGTAGCTTGC
TTGTGTGTTT
AGTTTATAAT
TAGTCTCTTA
TTAATTTGAT
GTAGGTAAGC
CCGCCACAAA
TATATATTTT
TAACAAGATA
CCGTGGAAAA
ACTTCGTGCT
ATCACAAAAC
AGTATACAAA
AAATAAGCTT
AACAATCTAT
TCTCCGCTTG
GTGATGCTAA
AGGGCTTTCA
ATAGACCTTG
TAAGTGAAGG
AGATGGAGCC
GTCAATCCGC
TACCCTGCCT
CTGGTCAGTT
GGTCTCAGCA
ATGTACCCTG
TGAGTCTTGA
TAGACTAGTT
GGTGACTAGT
CTCTGTAGAT
GGAACAAATG
GTGCTTGAGA
GGGCACGTAT
GGCAGAGCTC
CAGTCATCTG
CGGAACATAT
ACTGGGCCCG
GTACGCGTTA
AGCTTGGCAC
TGGCCGTCAG
CTGCTTCGTC
GATTAAACCC
TCAGCGCGGC
CGCATTTAAA
TCCCCACTTC
AGAAGTTCCT
ATACTTTCTA
GAGAATAGGA
ACTTCACTAT
AGAGTCGAAT
AAGGGCGACA
CCCCATAATT
AGCCCGGGCG
AAAGGCCCAG
TCTTTCGACT
GAGCCTTTCG
TTTTATTTGA
TGCCTGGCAG
TTCCCTACTC
TCGCATGGGG
AGTCCCCACA
CTACCATCGG
CGCTACGGCG
TTTCACTTCT
GAGTTCGGCA
TGGGGTCAGG
TGGGACCACC
GCGCTACTGC
CGCCAGGCAA
ACAAGGGGTG
TTATGAGCCA
TATTCAGGTA
TAAATGGGCT
CGCGATAATG
TTCAGAATTG
GTTAATTGGT
TGTAACACTG
ACCCCTATTT
GTTTATTTTT
CTAAATACAT
TCAAATATGT
ATCCGCTCAT
GAGACAATAA
CCCTGATAAA
TGCTTCAATA
ATATTGAAAA
AGGAAGAATA
TGAGTATTCA
ACATTTCCGT
GTCGCCCTTA
TTCCCTTTTT
TGCGGCATTT
TGCCTTCCTG
TTTTTGCTCA
CCCAGAAACG
CTGGTGAAAG
TAAAAGATGC
TGAAGATCAG
TTGGGTGCAC
GAGTGGGTTA
CATCGAACTG
GATCTCAACA
GCGGTAAGAT
CCTTGAGAGT
TTTCGCCCCG
AAGAACGTTT
TCCAATGATG
AGCACTTTTA
AAGTTCTGCT
ATGTGGCGCG
GTATTATCCC
GTATTGACGC
CGGGCAAGAG
CAACTCGGTC
GCCGCATACA
CTATTCTCAG
AATGACTTGG
TTGAGTACTC
ACCAGTCACA
GAAAAGCATC
TTACGGATGG
CATGACAGTA
AGAGAATTAT
GTAGCGCTGC
CATAACCATG
AGTGATAACA
CTGCGGCCAA
CTTACTTCTG
ACAACGATCG
GAGGACCGAA
GGAGCTAACC
GCTTTTTTGC
ACAACATGGG
GGATCATGTA
ACTCGCCTTG
ATCGTTGGGA
ACCGGAGCTG
AATGAAGCCA
TACCAAACGA
CGAGCGTGAC
ACCACGATGC
CTGTAGCGAT
GGCAACAACG
TTGCGCAAAC
TATTAACTGG
CGAACTACTT
ACTCTAGCTT
CCCGGCAACA
ATTAATAGAC
TGGATGGAGG
CGGATAAAGT
TGCAGGACCA
CTTCTGCGCT
CGGCCCTTCC
GGCTGGCTGG
TTTATTGCTG
ATAAATCCGG
AGCCGGTGAG
CGTGGTTCTC
GCGGTATCAT
CGCAGCGCTG
GGGCCAGATG
GTAAGCCCTC
CCGTATCGTA
GTTATCTACA
CGACGGGGAG
TCAGGCAACT
ATGGATGAAC
GAAATAGACA
GATCGCTGAG
ATAGGTGCCT
CACTGATTAA
GCATTGGTAA
GCAGAGCATT
ACGCTGACTT
GACGGGACGG
CGCAAGCTCA
TGACCAAAAT
CCCTTAACGT
GAGTTACGCG
CGCGTCGTTC
CACTGAGCGT
CAGACCCCGT
AGAAAAGATC
AAAGGATCTT
CTTGAGATCC
TTTTTTTCTG
CGCGTAATCT
GCTGCTTGCA
AACAAAAAAA
CCACCGCTAC
CAGCGGTGGT
TTGTTTGCCG
GATCAAGAGC
TACCAACTCT
TTTTCCGAAG
GTAACTGGCT
TCAGCAGAGC
GCAGATACCA
AATACTGTTC
TTCTAGTGTA
GCCGTAGTTA
GCCCACCACT
TCAAGAACTC
TGTAGCACCG
CCTACATACC
TCGCTCTGCT
AATCCTGTTA
CCAGTGGCTG
CTGCCAGTGG
CGATAAGTCG
TGTCTTACCG
GGTTGGACTC
AAGACGATAG
TTACCGGATA
AGGCGCAGCG
GTCGGGCTGA
ACGGGGGGTT
CGTGCACACA
GCCCAGCTTG
GAGCGAACGA
CCTACACCGA
ACTGAGATAC
CTACAGCGTG
AGCTATGAGA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCTT
TGAGTGAGCT
GATACCGCTC
GCCGCAGCCG
AACGACCGAG
CGCAGCGAGT
CAGTGAGCGA
GGAAGCGGAA
GGCGAGAGTA
GGGAACTGCC
AGGCATCAAA
CTAAGCAGAA
GGCCCCTGAC
GGATGGCCTT
TTTGCGTTTC
TACAAACTCT
TTCTGTGTTG
TAAAACGACG
GCCAGTCTTA
AGCTCGGGCC
CCCTGGGCGG
TTCTGATAAC
GAGTAATCGT
TAATCCGCAA
ATAACGTAAA
AACCCGCTTC
GGCGGGTTTT
TTTATGGGGG
GAGTTTAGGG
AAAGAGCATT
TGTCAGAATA
TTTAAGGGCG
CCTGTCACTT
TGCTTGATAT
ATGAGAATTA
TTTAACCTTA
TAAATGAGAA
AAAAGCAACG
CACTTTAAAT
AAGATACGTT
GCTTTTTCGA
TTGATGAACA
CCTATAATTA
AACTATTCAT
CTATTATTTA
TGATTTTTTG
TATATACAAT
ATTTCTAGTT
TGTTAAAGAG
AATTAAGAAA
ATAAATCTCG
AAAATAATAA
AGGGAAAATC
AGTTTTTGAT
ATCAAAATTA
TACATGTCAA
CGATAATACA
AAATATAATA
CAAACTATAA
GATGTTATCA
GTATTTATTA
TGCATTTAGA
ATAAATTTTG
TGTCGCCCTT
ATTCGACTCA
CTATAGAAGT
TCCTATTCTC
TAGAAAGTAT
AGGAACTTCA
CTTCATTTTA
TTTAAATTTG
CGGCCGCGCT
GAGGTCTTAA
TGCGGTAGTG
GGGATGCCTC
AATTGTGCTA
GCTGCGGCCG
CCTTGCTAGA
TGACTGGTAG
GAATCTAACA
AAGGGAAAAC
CTTGACGATT
GGGGGCGGAA
AACATGGCAT
TACAGGGGCC
AATACGGACG
AGCTCATCGT
TTAATGACAG
GCACAATGTA
TGCAGCCTTC
CTCCACAACA
CTCGTACTGG
CACTACACTT
TTGCTCCAAG
AGGAAATCAT
GACTTGCCGC
ATACTCTGGC
CATATAAGCT
TCCCAGCCTT
CTTTCTGGTA
CCGCTGTGCA
GCTTCAACCG
GGTCGGGAGC
AGCGTAGATG
CCTCGACCGG
CGATGATAAA
GTCGGCACCG
CGTCCAATAG
CCGATGCAGG
AGTCTGGTAT
TGCTGTCCAA
GCTTATCTCC
TTTGGAAGAG
AGGTTCACAC
CCGTCGTGAA
GACCACGAAA
TCTTCATCCT
CCGAGGCTGA
AGACACATCC
GACTGCACTT
CCGTCAGGGC
CCGCGTCGAC
ACGAAACCCA
TAACGAAGTT
CTTGTATTTG
CGAGCGTAGT
CAACCGATGC
CTTGGTATAC
TCGCCCGTAG
CCAGCGATCC
TTTGGGGGTC
ATCTCTGCCA
GGACCAACAG
TCCTCTCTCA
GGACCATAGG
GGAAGTCTTG
CGCAGATGCG
GTCTGGGCCA
GAGCCTCGAC
GATGCCCTCG
CCAGGGAGAA
CGCTGCAGTT
GATAATGTGG
GCCCATTCGG
AGATCCTCAG
AGCACCGCCG
TGGTATTGCT
TCTGGACGGT
ATTGCCGATG
TCGATGAATT
TGCGGTCCTC
GAAGATCAAA
AAGTTGTACT
TTTGAGCCAG
CACATTCAGG
CCATTGATAG
TGTCGACGCT
GAAATCGGTG
AGGATGTCGA
TGTGTGTCTT
GATGACGGCG
ATGTAGGGAC
CGAGACCTGT
ATCATCAAAA
GTTGATTAGT
CCATTGCTAG
ACGGCATATG
TATTGGATCC
AACAGCTTCC
GTACGGTCAG
CGAGGTCCAG
GAGTTCTCGG
GTTGTCGTCA
CATCAGCAGA
GACGGTAACG
TTTGTCTTCT
TTGCTTCGGC
AATCTCAAAA
AGTCTCTTTG
CCAGAGGATT
GGGGTGCTTG
CTGGCTCGAG
CACCGTAAGT
CAATTGCGAC
TTGGACGACA
TCGTGGGAAT
GGAGGGTTAT
ATGCGGGGTA
TGACGGTGAT
TGATGAGTTG
AAGTAGCCGA
GCAATGAGGT
ATATTATCCA
ATTGAGGGTG
ACCCAACCAA
ATCAATTGCT
TGAGAATCAA
ATCTCAAATA
TTCTAAAATA
GAAGCCGCCG
GGGACTAAAT
TTAAAGTCGT
ACTCTATCTC
GATCTTTTCC
GCGTGTAAAA
ATTGCGTGCC
AATCTGGATG
GAGACAGGCC
ACATCGGTGC
TGTATTCCTC
CGCCTCCGAC
TGTGGGGTCA
ACCACAATTA
AACTACTCTG
TAGTCAGGTA
CAGCTAGAAT
GGGGTAGACA
GGCAGAACTG
TAGAAGATAA
AACATTGGTC
AATCACTGGT
AACTCCACGT
CTAGAGGATC
CCCGGCCGGG
TACCGAGCTC
GAATTCGTAA
TCATGTCATA
TTACCTAGTG
GGCGCTTACA
CAGTACACGA
GGAGCTGAGG
TCTTAATGTA
TTGGGATGAA
TTTTGTATGC
ACGCGCTGCG
GGCGCAATTT
TTTGAAATTT
TCCATTTTTA
ATGTCTTGAA
TCGCGCATTG
GATCCTGCCC
TCTTGCTCAA
TCAAATCTCG
CTTTGTTTTA
CGCGCTTTTG
GTGGCATGAT
GGTTGTTGAA
AGTTGAGGTT
GATAAACGCG
TTGGGGTGGA
CGAGAAAACT
ACCTTCCGCC
CGGGACGCAC
CTACCGCCCG
GGATTTACGT
TATGACTTTC
GCGCAGAACT
CTGTCCAGGT
CATGTAATAT
CACGTGATAA
GCAAGGTACA
CGCAAAACGA
GGTACATTTA
CGAATAGTTT
CAACATTGGC
TTTACGGGAA
GAGCTGAGAT
TAATAATGAA
CAGCGGATTT
GAAGTTCAGG
AATCATAATC
CATTGTAGAA
TCAACTACAT
AACAAGTCTA
ATTCCATTTC
TTTCATTCGT
AAAGGCTTTT
TTAAGGAAGT
CATAACGGCA
TAAATCGAAT
GTCCGCAGTA
GACTTTCAGA
CCTTGCGCTT
CTTCTTGGGA
GGGGCATAAT
CCGGGACGTC
GTACGGATAG
GCGTAGTCAG
GCACGTCGTA
AGGGTAAGCG
TAATCGGGGA
CATCGTAGGG
GTACGAGCCT
TTCTTCTTCT
TTGCCTGGCC
AGCCTTCTTG
GTGGCGGCAG
GGCGCTTGTG
CTTGACGGAG
GTCTGGGCGT
ACTCGAGCCA
CTCTTTGTTG
GAAATGGCGA
TCTTGACCTT
ATCGAGCTTC
TCGTCCTCAG
CTTTCTTGAA
CTGGCCGATG
GCCCAGAGGA
CCTTGCGGGC
GATATTGTAG
GCGCCGTTGG
CGTCGGCGTT
CTTGGGCAAG
ATGGCGTTCT
CTTGAGCCTC
GTAGTTCCGG
GAATCGTAGA
AGATACCATC
CGAGTTCTTG
ACAGGGGAGA
TGAGGAAATC
GACATCGGTC
CGGCCGGTGA
TGGAGTTGCG
CATCTGGAGC
ATCAGGGACA
TGAGGGCCAT
GAAGGAGGAG
TAGAAGGCCT
TATCGGACTG
CTCGCAGAGC
AGAGCGCGGA
TGTCGCCCTG
CTGGTAGTTG
ATGCCGTACT
TGTTGAACAG
TTCTTTGTAG
GCGGAGGTGA
GGCAGACTTC
TTCCCAATCG
AAAACGTTGT
TCTTCTTAGG
ATTGCGGAAG
ATGCGGATGC
GGTTGCCGTA
GGAGTAGAGC
TTCCACTTCT
TAATGTAATC
GGCGTCGGTG
CGGCTGAAGT
TCTTATAATC
CAGGGCGAAC
TCGAAGAGAT
CTTCCTCGGG
GACGTACATG
ATGCGATCGA
AGCTGGAGAT
GAACTTCTTG
GAATCGGCAA
TGGAGGTGTA
CTTGGTCTTG
AGCAGGTTCA
CGAAGCCGGT
CGAGGGATCG
ATCTTGGAGG
TGAGCCAGGC
GGGGATGTAG
AAGATGAAAC
CGTTCTGGGT
GCTCATGGAC
TTGAACGACT
CGAACTTGTT
AGTGATCTGG
TAGCCCTTGA
GGGCTCCGCC
GGTGGCGCAG
GGGTTCGACT
TCTTGTCGAC
CATGTAGTTG
AGCTTGTCGA
TGAGCATCTT
CTCGAACTTT
TGGTAGACCT
GCTTCTCAAC
CTTGACGCGG
CTGTTCTTGA
AGCCGGAGTT
CAGATCCTCG
AGAGCGATCA
CGGCATCATA
CTTCTCGACC
AGCTCGCAGA
TCTTGTGGAC
GACCTGCGAG
ATGTAGCCGG
CCTTCAACTC
TTTGATGTTC
TCGATGGAGG
TCCAGTTCTG
GCGGGCCTCG
AAGCGCTCTT
TTTCTTTCTT
ATCGAGGAGG
CTGTGGTAGT
CGGTCTTGAT
GCGGATACCG
TTGAAGTTGT
TGATGATTTC
GTTCAGGGAG
TACTGCTCGA
CGATGTTACC
CTTGCCATCG
ACGACGACGA
TGTAGAGGAG
GTTGCGCTCG
CCGCGATCGA
TACCGATGAC
GTAGGGGTTG
TCATCGTGCT
TCAGGAGGAC
GCGGACCTCG
GTATTGATCT
TGAAGATGTT
CTTAGGGCAC
TTGTTGATGG
CGATAGGGAT
GTGCAGCTCG
TACTGATCCT
CGCTGAAGCG
CTTGTCCTTG
TAGACGTCGT
AGGACAAGGT
GGTAGTTTTC
TTGGGGTTAT
CAGGATTCTT
GTTGGCGATC
GGGGAGTTAG
CAGGATGGAC
GACCAACTCT
TCTTTCTTCA
GGCTAGCACG
GCGCATGAAC
AGCTCGGCAC
CGCCGGAGAG
GCGGATCTGG
CCGTGGTTGT
TCTCGTCGAA
CAGCAGCTTG
AAGTACATGG
TGTGGAGGTT
AGGGGTGCCG
TGGGACTTAT
CGGAGAAATC
CTTGTTGTAG
ATTTGGAACA
TGTAGAGCTT
GCCCTCTTCG
ACGAGCTTAT
CGACCTCTTT
CTTGGAGGCG
GACTCGAAGG
ACACCTTGTA
ACCCTGTTCC
TCGACCTCAC
GGTAGAAGCC
AGCGATATCC
TTATACTTCT
CAGTCTCGGA
GAAGTTGAAA
TCGTAGGCGT
TGGACCACTT
GGGGTAGCGC
GAGATGGAAT
CCTTGAAGAA
ATCGATGAGC
TTGTGGCAAT
CGTTGAGGTT
GAACATATCG
CCCTTTTTGA
AGGTGCCGTT
CTTATAGATC
TTCTGGATAT
CCTCGGAGGG
GTTGTAGTAG
GCCATCCATT
TCTTGCTGAA
GAACACCTTG
GGCAGCATCT
TGTTAGGACC
GGGGAGCAGC
TTGTAGTTGA
TCTTTTCGTA
GTTGCCGTTG
ACATCGTCCT
TATCGATCTT
TTGGAGGCAC
TTGGCGTATT
TCTTATCCAT
GATGGCGAGG
TAGTACTTGC
TGCCGTAGCG
GAGGATGGTG
GCGCGGTAAT
CGGTTTCTTT
GTCTTTGTCC
CAGCCGCCCA
TGAACTGAGG
ATTCTGGAAG
TACAACTTGA
ACTTATCCTT
GGAGTAAGGC
TTCTGGGTGA
CGTAGTTGCG
GATAGCATCG
TAGATGTGAT
CGACCTTGAG
GAGGATATCG
TAGGCCAGGA
CGAAATCACC
GTAGAAGCTC
TCGTCGCGGT
TAGTCTCTTT
GCCCTCACCG
AAGAAAGCCT
TGATGTAGTT
CTCGAACGAC
TTCACGGAAT
CGAGCAGATC
CTTCATGATA
GCGACGACGG
CGTCGTTCTT
CTTCAGGGAC
TTTTCGAGGA
CGAAGTCGGC
ATCGAACAGC
TTCTCAGAGG
AGCCGTAGAC
CTTGTAGATC
TCGTCCACCT
TCTGGATGAT
GATCTCTTTC
AGCTTCTCGA
CGACGGAGAG
ATCGGCATCG
GCGTACTCTT
GGAGCTGCTC
GAGGCTGAAG
GAGCCGATCT
TCTTGAAGGA
CTTGCGGCGA
TCATCCTCGT
ACTTCTCGGT
AACGACGGCC
TTCTTCTTGA
GGTGGATGTC
ATCGTACTCG
GCGTTCCACT
TATCGCGGAT
GACGTTCCAC
TCGCCGAAGA
TGTCCTTGGA
GATGGTGGAG
ATAGCAGGGC
CGTTCTTGAC
GAAGATGCCG
GCGGAGGAGT
ACTCATCGAA
GTTCTTGAAA
AGCTTCTCCA
ACTTCTTGAT
GCTGGAGAAG
ATCTCGGAGT
TCTTGTTGAG
GGTGTTGCGA
AAGACCTCGA
GGACTTCCTC
ATCGGAGGTG
TAACCCTCGC
CGTAGAAGGA
CAGGGACTCG
CGATCGGAGA
GGACCTGCTT
GTAGAGGGGC
TTGAACTTGG
GGAGCTTCTG
CTTGGTCTTT
TGATTGTAGA
GGTTGATGTA
CTCGTTGAGG
CCCTTGATCT
TCTCGCCGGA
CTCGGTGACG
AAGCCGCCGA
TGATGGCGTT
GTAGACATCG
ATGCCCTCTT
GGGTGAGCAC
GAAGTTGAAG
AACTCGCCCT
CGAAGAAATC
CTCGACATCG
TAATCGGAGT
TGAGGATCTT
TTCTTTGATC
TCTTGGACCT
CGTGCTTGTC
GAAGATGGCA
TCGACCTTCT
CGAAGATATC
CATGTTGCTG
ATGTAGCGGG
TCAAGTTCTC
GTTGATGCAG
CGGAAGGCGA
TAGAGGTGGA
CTTGGCTTCC
TCGGAGAACA
TGTTTTCGCG
GTTATCGAAG
AAACCGGTGA
AGGCGGTAGT
GAAGCCGTTG
AAGGAGTTGA
CGAGGGCGAT
CTCATCCTTA
TCATCGAGGA
ACTCAGGCAG
GATGGTTTCG
ATGATATCCT
TCTTGAACAG
GGACTTGTAG
CCCTCGTTGC
CCTTGAAGGC
CTTGGCGATC
TCTTTGCGGA
GGTTGATCTC
GAGGTTTTCC
AGCTCTTTGT
TCTCTTTCTC
GGTGCGGGTT
TTCTTGCGGA
ACAGGGAGAT
GTAGTTGTTG
AGGTTCTTGA
GCTTGATGGA
GTGGAGGACA
TCGTTGATGA
AGGAGAGGTA
GTAGCGATCG
AGGAGCTTCT
TGACGCCCTT
GTAATCCTCG
GCGCGCTTCT
CATCCTCGAC
GAGGAGGCGC
TTGTTATCGA
TATTCTCTTG
GGTCTTGCCG
ACGGGGATAG
CCTTGAAGCG
GAGGGTCTTG
CTGAGGGAGT
AGCAGTTGGT
GAACTTCTCG
AGCTTGGACA
TACGAAGTCT
GTGTTATGTT
TTGTGGAATA
TAAAAGGGAG
AAGTGCTCAG
ATACTACGGC
TAGTAATGCG
TAGAGGTGCT
GAGCAGAGAA
GTTAGCAGGA
GATGGATGAA
AGAAGGATGG
AGGAGAGAAG
CGAGGGAAGG
GAAGATATAC
GTACTGAAGC
GGACAAGGAG
AGGAAGGGAA
AGAAAATTTT
GTGGGAGGGG
GAGGGGAAAT
CCTTTTATGG
AGGGGCAAGC
GAGGACGACG
ACTAAGCGGG
CGGGCTCAAA
CCCTTGGCTG
GTAGATATCT
GTCATTGGAT
ATGGAGGGGT
AACCCGCCCC
ACTCCCGATG
CAAATTCTTC
TCCTAGACTC
CCACCTTGGG
CGTCGAGCCT
TTACAACACC
TTCCGGAACG
GCAGCCCAGC
GCGACTCTTT
CCTTGGTTCT
GCTCCACCCC
ATCTCGTTTT
CATGGCTTAG
GGGCAGAAAT
TTTTTTTTGT
TTAGCTTTTC
TTTACTTGCT
ATCGCCTCTA
CGGTGGATTC
TCGGATCGAA
AAAGCTGGGC
ATCAACGCGT
TGATGGTCGC
ATGGCGGCTT
TTATCACTCT
GTACTTTGCA
GCGGACAACC
CCGCGGCTGT
ACGAGTTTGC
CGCCTTTTAA
TTTGTCTGCG
ACCAGCCTGC
GATGGAGCGA
GCGAGTGGCG
TTGCTTGATC
CACAGCGTCA
AGATCAA