名称: | pYPQ-dzCas9-Act3.0 |
---|---|
别称: | Plasmid#158414 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Spe |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTATCTGGGG
CGACGGAATA
ACAGATTATA
ACTCTGCTTT
GAAGGACAGA
TTCATCATTA
GCAAGGACAA
CGGGAAGAAC
ACGGTGTACC
TCCAGATGAG
CAAAGTGCGC
AGTGATGATA
CTGCCCTGTA
TTACTGCGTC
ACAGGTTTGT
TTGATTACTG
GGGTCAAGGC
ACTCTTGTGA
CAGTCTCTTC
GTATCCTTAT
GACGTGCCCG
ACTACGCTGG
CGGAGGGGGC
GGCAGCGGAG
GCGGTGGATC
CGGAGGCGGT
GGCTCAGGGG
GCGGCGGGTC
GCTTGATCCT
GGTGGCGGAG
GTTCTGGGAG
CAAAGGAGAG
GAGCTCTTTA
CAGGTGTCGT
CCCTATTTTG
GTGGAGCTTG
ATGGAGATGT
CAATGGCCAT
AAATTTTCCG
TCAGGGGCGA
AGGGGAGGGG
GACGCGACTA
ATGGAAAATT
GACCCTCAAG
TTTATCTGCA
CGACAGGAAA
GTTGCCGGTC
CCCTGGCCCA
CTCTGGTTAC
CACCCTCACT
TATGGGGTCC
AGTGCTTTAG
CAGATACCCT
GATCATATGA
AACGCCATGA
CTTCTTCAAA
TCAGCCATGC
CAGAGGGTTA
TGTGCAGGAA
AGAACTATAA
GCTTTAAGGA
CGATGGGACG
TACAAAACGC
GGGCAGAGGT
TAAGTTTGAG
GGGGATACTC
TTGTGAATAG
GATCGAACTT
AAGGGCATCG
ATTTTAAAGA
AGACGGTAAT
ATTCTTGGCC
ACAAGTTGGA
GTATAATTTC
AATTCTCACA
ACGTGTACAT
AACAGCAGAT
AAACAGAAAA
ATGGGATCAA
GGCCAATTTT
AAAATTAGGC
ATAATGTTGA
AGATGGGTCT
GTGCAACTGG
CAGATCATTA
TCAGCAGAAT
ACTCCAATTG
GCGATGGCCC
TGTCTTGTTG
CCTGACAATC
ATTACCTTTC
CACGCAGTCA
GTCCTGTCCA
AAGATCCGAA
TGAGAAGCGG
GACCACATGG
TCTTGCTCGA
ATTCGTTACT
GCCGCCGGAA
TCACTCATGG
CATGGACGAG
CTTTACAAGG
GAGGTGGAAG
GACTGGAGGT
GGCGGAGGGG
GGCTGCTTGA
CCCCGGAACC
CCTATGGATG
CCGACTTGGT
GGCCTCCTCC
ACGGTTGTTT
GGGAACAGGA
TGCCGACCCT
TTTGCGGGCA
CTGCTGACGA
TTTTCCCGCT
TTCAATGAGG
AGGAGTTGGC
TTGGCTGATG
GAACTTCTCC
CACAGGGTGG
TAGTGGTGGC
CTCCTGGATC
CGGGGACACC
GATGGACGCA
GACCTGGTGG
CGTCGTCAAC
GGTTGTGTGG
GAACAGGATG
CTGATCCGTT
CGCAGGAACT
GCCGACGATT
TTCCTGCATT
CAACGAAGAG
GAACTCGCAT
GGCTCATGGA
GCTTCTGCCT
CAGGGATCGG
GTGGCGGGTC
TCGAACGGAA
GAATACAAGC
TTATATTGAA
CGGTAAGACA
CTGAAAGGTG
AAACTACGAC
AGAAGCCGTT
GACGCGGCAA
CTGCGGAGAA
AGTCTTTAAG
CAGTATGCTA
ACGATAATGG
CGTGGACGGG
GAGTGGACGT
ATGACGACGC
AACCAAAACA
TTCACGGTCA
CCGAGGGGGG
CGGCAGCGGA
GGCGGTACCT
CGCCCAAAAC
CCGGAGACGC
CCTAGGCGCT
CGCAGCGCAA
AAGACCTCCC
ACGTAAAGAG
CTTTCGTTCG
TATCATCGGT
TTCGACAACG
TTCGTCAAGT
TCAATGCATC
AGTTTCATTG
CGCACACACC
AGAATCCTAC
TGAGTTTGAG
TATTATGGCA
TTGGGAAAAC
TGTTTTTCTT
GTACCATTTG
TTGTGCTTGT
AATTTACTGT
GTTTTTTATT
CGGTTTTCGC
TATCGAACTG
TGAAATGGAA
ATGGATGGAG
AAGAGTTAAT
GAATGATATG
GTCCTTTTGT
TCATTCTCAA
ATTAATATTA
TTTGTTTTTT
CTCTTATTTG
TTGTGTGTTG
AATTTGAAAT
TATAAGAGAT
ATGCAAACAT
TTTGTTTTGA
GTAAAAATGT
GTCAAATCGT
GGCCTCTAAT
GACCGAAGTT
AATATGAGGA
GTAAAACACT
TGTAGTTGTA
CCATTATGCT
TATTCACTAG
GCAACAAATA
TATTTTCAGA
CCTAGAAAAG
CTGCAAATGT
TACTGAATAC
AAGTATGTCC
TCTTGTGTTT
TAGACATTTA
TGAACTTTCC
TTTATGTAAT
TTTCCAGAAT
CCTTGTCAGA
TTCTAATCAT
TGCTTTATAA
TTATAGTTAT
ACTCATGGAT
TTGTAGTTGA
GTATGAAAAT
ATTTTTTAAT
GCATTTTATG
ACTTGCCAAT
TGTTCCGGAA
CTCTAGATAA
GCTTACCGGA
AAGGGCGAAT
TCGCAACTTT
GTATACAAAA
GTTGAACGAG
AAACGTAAAA
TGATATAAAT
ATCAATATAT
TAAATTAGAT
TTTGCATAAA
AAACAGACTA
CATAATACTG
TAAAACACAA
CATATCCAGT
CACTATGCCA
TCCAGCTGAT
ATCCCCTATA
GTGAGTCGTA
TTACATGGTC
ATAGCTGTTT
CCTGGCAGCT
CTGGCCCGTG
TCTCAAAATC
TCTGATGTTA
CATTGCACAA
GATAAAAATA
TATCATCATG
CCTCCTCTGG
ACCAGCCAGG
ACAGAAATGC
CTCGACTTCG
CTGCTACCCA
AGGTTGCCGG
GTGACGCACA
CCGTGGAAAC
GGATGAAGGC
ACGAACCCAG
TGGACATAAG
CCTGTTCGGT
TCGTAAGCTG
TAATGCAAGT
AGCGTATGCG
CTCACGCAAC
TGGTCCAGAA
CCTTGACCGA
ACGCAGCGGT
GGTAACGGCG
CAGTGGCGGT
TTTCATGGCT
TGTTATGACT
GTTTTTTTGG
GGTACAGTCT
ATGCCTCGGG
CATCCAAGCA
GCAAGCGCGT
TACGCCGTGG
GTCGATGTTT
GATGTTATGG
AGCAGCAACG
ATGTTACGCA
GCAGGGCAGT
CGCCCTAAAA
CAAAGTTAAA
CATTATGAGG
GAAGCGGTGA
TCGCCGAAGT
ATCGACTCAA
CTATCAGAGG
TAGTTGGCGT
CATCGAGCGC
CATCTCGAAC
CGACGTTGCT
GGCCGTACAT
TTGTACGGCT
CCGCAGTGGA
TGGCGGCCTG
AAGCCACACA
GTGATATTGA
TTTGCTGGTT
ACGGTGACCG
TAAGGCTTGA
TGAAACAACG
CGGCGAGCTT
TGATCAACGA
CCTTTTGGAA
ACTTCGGCTT
CCCCTGGAGA
GAGCGAGATT
CTCCGCGCTG
TAGAAGTCAC
CATTGTTGTG
CACGACGACA
TCATTCCGTG
GCGTTATCCA
GCTAAGCGCG
AACTGCAATT
TGGAGAATGG
CAGCGCAATG
ACATTCTTGC
AGGTATCTTC
GAGCCAGCCA
CGATCGACAT
TGATCTGGCT
ATCTTGCTGA
CAAAAGCAAG
AGAACATAGC
GTTGCCTTGG
TAGGTCCAGC
GGCGGAGGAA
CTCTTTGATC
CGGTTCCTGA
ACAGGATCTA
TTTGAGGCGC
TAAATGAAAC
CTTAACGCTA
TGGAACTCGC
CGCCCGACTG
GGCTGGCGAT
GAGCGAAATG
TAGTGCTTAC
GTTGTCCCGC
ATTTGGTACA
GCGCAGTAAC
CGGCAAAATC
GCGCCGAAGG
ATGTCGCTGC
CGACTGGGCA
ATGGAGCGCC
TGCCGGCCCA
GTATCAGCCC
GTCATACTTG
AAGCTAGACA
GGCTTATCTT
GGACAAGAAG
AAGATCGCTT
GGCCTCGCGC
GCAGATCAGT
TGGAAGAATT
TGTCCACTAC
GTGAAAGGCG
AGATCACCAA
GGTAGTCGGC
AAATAACCCT
CGAGCCACCC
ATGACCAAAA
TCCCTTAACG
TGAGTTACGC
GTCGTTCCAC
TGAGCGTCAG
ACCCCGTAGA
AAAGATCAAA
GGATCTTCTT
GAGATCCTTT
TTTTCTGCGC
GTAATCTGCT
GCTTGCAAAC
AAAAAAACCA
CCGCTACCAG
CGGTGGTTTG
TTTGCCGGAT
CAAGAGCTAC
CAACTCTTTT
TCCGAAGGTA
ACTGGCTTCA
GCAGAGCGCA
GATACCAAAT
ACTGTTCTTC
TAGTGTAGCC
GTAGTTAGGC
CACCACTTCA
AGAACTCTGT
AGCACCGCCT
ACATACCTCG
CTCTGCTAAT
CCTGTTACCA
GTGGCTGCTG
CCAGTGGCGA
TAAGTCGTGT
CTTACCGGGT
TGGACTCAAG
ACGATAGTTA
CCGGATAAGG
CGCAGCGGTC
GGGCTGAACG
GGGGGTTCGT
GCACACAGCC
CAGCTTGGAG
CGAACGACCT
ACACCGAACT
GAGATACCTA
CAGCGTGAGC
TATGAGAAAG
CGCCACGCTT
CCCGAAGGGA
GAAAGGCGGA
CAGGTATCCG
GTAAGCGGCA
GGGTCGGAAC
AGGAGAGCGC
ACGAGGGAGC
TTCCAGGGGG
AAACGCCTGG
TATCTTTATA
GTCCTGTCGG
GTTTCGCCAC
CTCTGACTTG
AGCGTCGATT
TTTGTGATGC
TCGTCAGGGG
GGCGGAGCCT
ATGGAAAAAC
GCCAGCAACG
CGGCCTTTTT
ACGGTTCCTG
GCCTTTTGCT
GGCCTTTTGC
TCACATGTTC
TTTCCTGCGT
TATCCCCTGA
TTCTGTGGAT
AACCGTATTA
CCGCCTTTGA
GTGAGCTGAT
ACCGCTCGCC
GCAGCCGAAC
GACCGAGCGC
AGCGAGTCAG
TGAGCGAGGA
AGCGGAAGAG
CGCCCAATAC
GCAAACCGCC
TCTCCCCGCG
CGTTGGCCGA
TTCATTAATG
CAGCTGGCAC
GACAGGTTTC
CCGACTGGAA
AGCGGGCAGT
GAGCGCAACG
CAATTAATAC
GCGTACCGCG
AGCCAGGAAG
AGTTTGTAGA
AACGCAAAAA
GGCCATCCGT
CAGGATGGCC
TTCTGCTTAG
TTTGATGCCT
GGCAGTTTAT
GGCGGGCGTC
CTGCCCGCCA
CCCTCCGGGC
CGTTGCTTCA
CAACGTTCAA
ATCCGCTCCC
GGCGGATTTG
TCCTACTCAG
GAGAGCGTTC
ACCGACAAAC
AACAGATAAA
ACGAAAGGCC
CAGTCTTCCG
ACTGAGCCTT
TCGTTTTATT
TGATGCCTGG
CAGTTCCCTA
CTCTCGCGTT
AACGCTTGCA
TGGATGTTTT
CCCAGTCACG
ACGTTGTAAA
ACGACGGCCA
GTCTTAAGCT
CGGGCCCCAA
ATAATGATTT
TATTTTGACT
GATAGTGACC
TGTTCGTTGC
AACAAATTGA
TGAGCAATGC
TTTTTTATAA
TGCCAACTTT
GTACAAAAAA
GCAGGCTCCG
AATTCGCCCT
TCACCATGGA
TTACAAGGAC
CACGACGGGG
ATTACAAGGA
CCACGACATT
GATTACAAGG
ATGATGATGA
CAAGATGGCT
CCGAAGAAGA
AGAGGAAGGT
TGGCATCCAC
GGGGTGCCAG
CTGCTGACAA
GAAGTACTCG
ATCGGCCTCG
CTATTGGGAC
TAACTCTGTT
GGCTGGGCCG
TGATCACCGA
CGAGTACAAG
GTGCCCTCAA
AGAAGTTCAA
GGTCCTGGGC
AACACCGATC
GGCATTCCAT
CAAGAAGAAT
CTCATTGGCG
CTCTCCTGTT
CGACAGCGGC
GAGACGGCTG
AGGCTACGCG
GCTCAAGCGC
ACCGCCCGCA
GGCGGTACAC
GCGCAGGAAG
AATCGCATCT
GCTACCTGCA
GGAGATTTTC
TCCAACGAGA
TGGCGAAGGT
TGACGATTCT
TTCTTCCACA
GGCTGGAGGA
GTCATTCCTC
GTGGAGGAGG
ATAAGAAGCA
CGAGCGGCAT
CCAATCTTCG
GCAACATTGT
CGACGAGGTT
GCCTACCACG
AGAAGTACCC
TACGATCTAC
CATCTGCGGA
AGAAGCTCGT
GGACTCCACA
GATAAGGCGG
ACCTCCGCCT
GATCTACCTC
GCTCTGGCCC
ACATGATTAA
GTTCAGGGGC
CATTTCCTGA
TCGAGGGGGA
TCTCAACCCG
GACAATAGCG
ATGTTGACAA
GCTGTTCATC
CAGCTCGTGC
AGACGTACAA
CCAGCTCTTC
GAGGAGAACC
CCATTAATGC
GTCAGGCGTC
GACGCGAAGG
CTATCCTGTC
CGCTAGGCTC
TCGAAGTCTC
GGCGCCTCGA
GAACCTGATC
GCCCAGCTGC
CGGGCGAGAA
GAAGAACGGC
CTGTTCGGGA
ATCTCATTGC
GCTCAGCCTG
GGGCTCACGC
CCAACTTCAA
GTCGAATTTC
GATCTCGCTG
AGGACGCCAA
GCTGCAGCTC
TCCAAGGACA
CATACGACGA
TGACCTGGAT
AACCTCCTGG
CCCAGATCGG
CGATCAGTAC
GCGGACCTGT
TCCTCGCTGC
CAAGAATCTG
TCGGACGCCA
TCCTCCTGTC
TGATATTCTC
AGGGTGAACA
CCGAGATTAC
GAAGGCTCCG
CTCTCAGCCT
CCATGATCAA
GCGCTACGAC
GAGCACCATC
AGGATCTGAC
CCTCCTGAAG
GCGCTGGTCA
GGCAGCAGCT
CCCCGAGAAG
TACAAGGAGA
TCTTCTTCGA
TCAGTCGAAG
AACGGCTACG
CTGGGTACAT
TGACGGCGGG
GCCTCTCAGG
AGGAGTTCTA
CAAGTTCATC
AAGCCGATTC
TGGAGAAGAT
GGACGGCACG
GAGGAGCTGC
TGGTGAAGCT
CAATCGCGAG
GACCTCCTGA
GGAAGCAGCG
GACATTCGAT
AACGGCAGCA
TCCCACACCA
GATTCATCTC
GGGGAGCTGC
ACGCTATCCT
GAGGAGGCAG
GAGGACTTCT
ACCCTTTCCT
CAAGGATAAC
CGCGAGAAGA
TCGAGAAGAT
TCTGACTTTC
AGGATCCCGT
ACTACGTCGG
CCCACTCGCT
AGGGGCAACT
CCCGCTTCGC
TTGGATGACC
CGCAAGTCAG
AGGAGACGAT
CACGCCGTGG
AACTTCGAGG
AGGTGGTCGA
CAAGGGCGCT
AGCGCTCAGT
CGTTCATCGA
GAGGATGACG
AATTTCGACA
AGAACCTGCC
AAATGAGAAG
GTGCTCCCTA
AGCACTCGCT
CCTGTACGAG
TACTTCACAG
TCTACAACGA
GCTGACTAAG
GTGAAGTATG
TGACCGAGGG
CATGAGGAAG
CCGGCTTTCC
TGTCTGGGGA
GCAGAAGAAG
GCCATCGTGG
ACCTCCTGTT
CAAGACCAAC
CGGAAGGTCA
CGGTTAAGCA
GCTCAAGGAG
GACTACTTCA
AGAAGATTGA
GTGCTTCGAT
TCGGTCGAGA
TCTCTGGCGT
TGAGGACCGC
TTCAACGCCT
CCCTGGGGAC
CTACCACGAT
CTCCTGAAGA
TCATTAAGGA
TAAGGACTTC
CTGGACAACG
AGGAGAATGA
GGATATCCTC
GAGGACATTG
TGCTGACACT
CACTCTGTTC
GAGGACCGGG
AGATGATCGA
GGAGCGCCTG
AAGACTTACG
CCCATCTCTT
CGATGACAAG
GTCATGAAGC
AGCTCAAGAG
GAGGAGGTAC
ACCGGCTGGG
GGAGGCTGAG
CAGGAAGCTC
ATCAACGGCA
TTCGGGACAA
GCAGTCCGGG
AAGACGATCC
TCGACTTCCT
GAAGAGCGAT
GGCTTCGCGA
ACCGCAATTT
CATGCAGCTG
ATTCACGATG
ACAGCCTCAC
ATTCAAGGAG
GATATCCAGA
AGGCTCAGGT
GAGCGGCCAG
GGGGACTCGC
TGCACGAGCA
TATCGCGAAC
CTCGCTGGCT
CGCCAGCTAT
CAAGAAGGGG
ATTCTGCAGA
CCGTGAAGGT
TGTGGACGAG
CTGGTGAAGG
TCATGGGCAG
GCACAAGCCT
GAGAACATCG
TCATTGAGAT
GGCCCGGGAG
AATCAGACCA
CGCAGAAGGG
CCAGAAGAAC
TCACGCGAGA
GGATGAAGAG
GATCGAGGAG
GGCATTAAGG
AGCTGGGGTC
CCAGATCCTC
AAGGAGCACC
CGGTGGAGAA
CACGCAGCTG
CAGAATGAGA
AGCTCTACCT
GTACTACCTC
CAGAATGGCC
GCGATATGTA
TGTGGACCAG
GAGCTGGATA
TTAACAGGCT
CAGCGATTAC
GACGTCGATG
CTATCGTTCC
ACAGTCATTC
CTGAAGGATG
ACTCCATTGA
CAACAAGGTC
CTCACCAGGT
CGGACAAGAA
CCGGGGCAAG
TCTGATAATG
TTCCTTCAGA
GGAGGTCGTT
AAGAAGATGA
AGAACTACTG
GCGCCAGCTC
CTGAATGCCA
AGCTGATCAC
GCAGCGGAAG
TTCGATAACC
TCACAAAGGC
TGAGAGGGGC
GGGCTCTCTG
AGCTGGACAA
GGCGGGCTTC
ATCAAGAGGC
AGCTGGTCGA
GACACGGCAG
ATCACTAAGC
ACGTTGCGCA
GATTCTCGAC
TCACGGATGA
ACACTAAGTA
CGATGAGAAT
GACAAGCTGA
TCCGCGAGGT
GAAGGTCATC
ACCCTGAAGT
CAAAGCTCGT
CTCCGACTTC
AGGAAGGATT
TCCAGTTCTA
CAAGGTTCGG
GAGATCAACA
ATTACCACCA
TGCCCATGAC
GCGTACCTGA
ACGCGGTGGT
CGGCACAGCT
CTGATCAAGA
AGTACCCAAA
GCTCGAGAGC
GAGTTCGTGT
ACGGGGACTA
CAAGGTTTAC
GATGTGAGGA
AGATGATCGC
CAAGTCGGAG
CAGGAGATTG
GCAAGGCTAC
CGCCAAGTAC
TTCTTCTACT
CTAACATTAT
GAATTTCTTC
AAGACAGAGA
TCACTCTGGC
CAATGGCGAG
ATCCGGAAGC
GCCCCCTCAT
CGAGACGAAC
GGCGAGACGG
GGGAGATCGT
GTGGGACAAG
GGCAGGGATT
TCGCGACCGT
CAGGAAGGTT
CTCTCCATGC
CACAAGTGAA
TATCGTCAAG
AAGACAGAGG
TCCAGACTGG
CGGGTTCTCT
AAGGAGTCAA
TTCTGCCTAA
GCGGAACAGC
GACAAGCTCA
TCGCCCGCAA
GAAGGACTGG
GATCCGAAGA
AGTACGGCGG
GTTCGACAGC
CCCACTGTGG
CCTACTCGGT
CCTGGTTGTG
GCGAAGGTTG
AGAAGGGCAA
GTCCAAGAAG
CTCAAGAGCG
TGAAGGAGCT
GCTGGGGATC
ACGATTATGG
AGCGCTCCAG
CTTCGAGAAG
AACCCGATCG
ATTTCCTGGA
GGCGAAGGGC
TACAAGGAGG
TGAAGAAGGA
CCTGATCATT
AAGCTCCCCA
AGTACTCACT
CTTCGAGCTG
GAGAACGGCA
GGAAGCGGAT
GCTGGCTTCC
GCTGGCGAGC
TGCAGAAGGG
GAACGAGCTG
GCTCTGCCGT
CCAAGTATGT
GAACTTCCTC
TACCTGGCCT
CCCACTACGA
GAAGCTCAAG
GGCAGCCCCG
AGGACAACGA
GCAGAAGCAG
CTGTTCGTCG
AGCAGCACAA
GCATTACCTC
GACGAGATCA
TTGAGCAGAT
TTCCGAGTTC
TCCAAGCGCG
TGATCCTGGC
CGACGCGAAT
CTGGATAAGG
TCCTCTCCGC
GTACAACAAG
CACCGCGACA
AGCCAATCAG
GGAGCAGGCT
GAGAATATCA
TTCATCTCTT
CACCCTGACG
AACCTCGGCG
CCCCTGCTGC
TTTCAAGTAC
TTCGACACAA
CTATCGATCG
CAAGAGGTAC
ACAAGCACTA
AGGAGGTCCT
GGACGCGACC
CTCATCCACC
AGTCGATTAC
CGGCCTCTAC
GAGACGCGCA
TCGACCTGTC
TCAGCTCGGG
GGCGACAAGC
GGCCAGCGGC
GACGAAGAAG
GCGGGGCAGG
CGAAGAAGAA
GAAGAAGGGA
GACGGCTCTG
GATCGGGGTC
GGGTTCTGGC
TCAGTCGACG
ATGCTCTTGA
CGATTTTGAC
CTCGATATGC
TCGACGCTCT
TGATGATTTT
GATCTCGACA
TGCTCGATGC
ACTTGATGAC
TTTGACCTTG
ACATGCTCGA
CGCACTCGAT
GACTTCGACC
TCGACATGCT
TGAGGGCAGA
GGAAGTCTTC
TAACATGCGG
TGACGTGGAG
GAGAATCCCG
GCCCTATGGC
GTCAAATTTC
ACGCAGTTTG
TTTTGGTTGA
TAACGGCGGG
ACTGGCGACG
TTACAGTAGC
TCCATCAAAT
TTTGCGAACG
GAGTCGCTGA
GTGGATTAGC
TCAAATTCAA
GGTCCCAGGC
CTACAAGGTT
ACCTGTTCTG
TTAGGCAGAG
TTCTGCGCAA
AAAAGAAAAT
ATACCATCAA
GGTTGAAGTC
CCTAAAGTTG
CAACACAAAC
AGTCGGTGGT
GTTGAGCTCC
CTGTGGCAGC
CTGGAGATCT
TACTTAAACA
TGGAGCTAAC
AATTCCAATA
TTCGCTACAA
ACTCTGATTG
TGAACTGATT
GTTAAGGCGA
TGCAAGGTCT
CTTGAAAGAT
GGAAACCCTA
TACCGTCCGC
TATCGCAGCT
AACAGCGGTA
TCTATCCTAA
GAAGAAGAGG
AAGGTTGGCT
CTGGATCGGG
GTCGGGTTCT
GGCTCAGGAT
CCGGTACCTA
TCCCTATGAC
GTGCCCGATT
ATGCCAGCCT
GGGCAGCGGC
TCCCCCAAGA
AAAAACGCAA
GGTGGAAGAT
CCTAAGAAAA
AGCGGAAAGT
GGACGGCATT
GGTAGTGGGA
GCAACGGCAG
CAGCGGATCC
AACGGTCCGA
CTGACGCCGC
GGAAGAAGAA
CTTTTGAGCA
AGAATTATCA
TCTTGAGAAC
GAAGTGGCTC
GTCTTAAGAA
AGGTTCTGGC
AGTGGAGAAG
AACTGCTTTC
AAAGAATTAC
CACCTGGAAA
ATGAGGTAGC
TAGACTGAAA
AAGGGGAGCG
GAAGTGGGGA
GGAGTTGCTG
AGCAAAAATT
ATCATTTGGA
GAACGAAGTA
GCACGACTAA
AGAAAGGGTC
CGGATCGGGT
GAGGAGTTAC
TCTCGAAAAA
TTATCATCTC
GAAAACGAAG
TGGCTCGGCT
AAAAAAGGGC
AGTGGTTCTG
GAGAAGAGCT
ATTATCTAAA
AACTACCACC
TCGAAAATGA
GGTGGCACGC
TTAAAAAAGG
GAAGTGGCAG
TGGTGAAGAG
CTACTATCCA
AGAATTATCA
TCTTGAGAAC
GAGGTAGCGC
GTTTGAAGAA
GGGTTCCGGC
TCAGGAGAGG
AACTGCTCTC
GAAGAACTAT
CATCTTGAAA
ATGAGGTCGC
TCGATTAAAA
AAGGGATCGG
GCAGTGGTGA
GGAACTACTT
TCAAAGAATT
ACCACCTCGA
AAACGAAGTA
GCTCGATTAA
AGAAAGGTTC
AGGGTCGGGT
GAAGAATTAC
TGAGTAAAAA
TTATCATCTG
GAAAATGAGG
TAGCGAGACT
AAAAAAGGGG
AGTGGTTCTG
GCGAGGAATT
GCTATCGAAA
AATTATCATC
TTGAGAACGA
AGTTGCTAGG
CTCAAAAAGG
GCTCAGGCTC
AGGCACCGCG
GTAAACATAG
GTGGTGGAAC
CGGTCCGATG
GATCTACAGC
GGCCGCAAGG
TGGAGGTGGA
CCCAAGAAGA
AGCGCAAGGT
GTGAACTAGT
TAAAGAGCTT
TCGTTCGTAT
CATCGGTTTC
GACAACGTTC
GTCAAGTTCA
ATGCATCAGT
TTCATTGCGC
ACACACCAGA
ATCCTACTGA
GTTTGAGTAT
TATGGCATTG
GGAAAACTGT
TTTTCTTGTA
CCATTTGTTG
TGCTTGTAAT
TTACTGTGTT
TTTTATTCGG
TTTTCGCTAT
CGAACTGTGA
AATGGAAATG
GATGGAGAAG
AGTTAATGAA
TGATATGGTC
CTTTTGTTCA
TTCTCAAATT
AATATTATTT
GTTTTTTCTC
TTATTTGTTG
TGTGTTGAAT
TTGAAATTAT
AAGAGATATG
CAAACATTTT
GTTTTGAGTA
AAAATGTGTC
AAATCGTGGC
CTCTAATGAC
CGAAGTTAAT
ATGAGGAGTA
AAACACTTGT
AGTTGTACCA
TTATGCTTAT
TCACTAGGCA
ACAAATATAT
TTTCAGACCT
AGAAAAGCTG
CAAATGTTAC
TGAATACAAG
TATGTCCTCT
TGTGTTTTAG
ACATTTATGA
ACTTTCCTTT
ATGTAATTTT
CCAGAATCCT
TGTCAGATTC
TAATCATTGC
TTTATAATTA
TAGTTATACT
CATGGATTTG
TAGTTGAGTA
TGAAAATATT
TTTTAATGCA
TTTTATGACT
TGCCCGTACG
CTGCAGTGCA
GCGTGACCCG
GTCGTGCCCC
TCTCTAGAGA
TAATGAGCAT
TGCATGTCTA
AGTTATAAAA
AATTACCACA
TATTTTTTTT
GTCACACTTG
TTTGAAGTGC
AGTTTATCTA
TCTTTATACA
TATATTTAAA
CTTTACTCTA
CGAATAATAT
AATCTATAGT
ACTACAATAA
TATCAGTGTT
TTAGAGAATC
ATATAAATGA
ACAGTTAGAC
ATGGTCTAAA
GGACAATTGA
GTATTTTGAC
AACAGGACTC
TACAGTTTTA
TCTTTTTAGT
GTGCATGTGT
TCTCCTTTTT
TTTTGCAAAT
AGCTTCACCT
ATATAATACT
TCATCCATTT
TATTAGTACA
TCCATTTAGG
GTTTAGGGTT
AATGGTTTTT
ATAGACTAAT
TTTTTTAGTA
CATCTATTTT
ATTCTATTTT
AGCCTCTAAA
TTAAGAAAAC
TAAAACTCTA
TTTTAGTTTT
TTTATTTAAT
AATTTAGATA
TAAAATAGAA
TAAAATAAAG
TGACTAAAAA
TTAAACAAAT
ACCCTTTAAG
AAATTAAAAA
AACTAAGGAA
ACATTTTTCT
TGTTTCGAGT
AGATAATGCC
AGCCTGTTAA
ACGCCGTCGA
CGAGTCTAAC
GGACACCAAC
CAGCGAACCA
GCAGCGTCGC
GTCGGGCCAA
GCGAAGCAGA
CGGCACGGCA
TCTCTGTCGC
TGCCTCTGGA
CCCCTCTCGA
GAGTTCCGCT
CCACCGTTGG
ACTTGCTCCG
CTGTCGGCAT
CCAGAAATTG
CGTGGCGGAG
CGGCAGACGT
GAGCCGGCAC
GGCAGGCGGC
CTCCTCCTCC
TCTCACGGCA
CCGGCAGCTA
CGGGGGATTC
CTTTCCCACC
GCTCCTTCGC
TTTCCCTTCC
TCGCCCGCCG
TAATAAATAG
ACACCCCCTC
CACACCCTCT
TTCCCCAACC
TCGTGTTGTT
CGGAGCGCAC
ACACACACAA
CCAGATCTCC
CCCAAATCCA
CCCGTCGGCA
CCTCCGCTTC
AAGGTACGCC
GCTCGTCCTC
CCCCCCCCCC
CCCTCTCTAC
CTTCTCTAGA
TCGGCGTTCC
GGTCCATGGT
TAGGGCCCGG
TAGTTCTACT
TCTGTTCATG
TTTGTGTTAG
ATCCGTGTTT
GTGTTAGATC
CGTGCTGCTA
GCGTTCGTAC
ACGGATGCGA
CCTGTACGTC
AGACACGTTC
TGATTGCTAA
CTTGCCAGTG
TTTCTCTTTG
GGGAATCCTG
GGATGGCTCT
AGCCGTTCCG
CAGACGGGAT
CGATTTCATG
ATTTTTTTTG
TTTCGTTGCA
TAGGGTTTGG
TTTGCCCTTT
TCCTTTATTT
CAATATATGC
CGTGCACTTG
TTTGTCGGGT
CATCTTTTCA
TGCTTTTTTT
TGTCTTGGTT
GTGATGATGT
GGTCTGGTTG
GGCGGTCGTT
CTAGATCGGA
GTAGAATTAA
TTCTGTTTCA
AACTACCTGG
TGGATTTATT
AATTTTGGAT
CTGTATGTGT
GTGCCATACA
TATTCATAGT
TACGAATTGA
AGATGATGGA
TGGAAATATC
GATCTAGGAT
AGGTATACAT
GTTGATGCGG
GTTTTACTGA
TGCATATACA
GAGATGCTTT
TTGTTCGCTT
GGTTGTGATG
ATGTGGTGTG
GTTGGGCGGT
CGTTCATTCG
TTCTAGATCG
GAGTAGAATA
CTGTTTCAAA
CTACCTGGTG
TATTTATTAA
TTTTGGAACT
GTATGTGTGT
GTCATACATC
TTCATAGTTA
CGAGTTTAAG
ATGGATGGAA
ATATCGATCT
AGGATAGGTA
TACATGTTGA
TGTGGGTTTT
ACTGATGCAT
ATACATGATG
GCATATGCAG
CATCTATTCA
TATGCTCTAA
CCTTGAGTAC
CTATCTATTA
TAATAAACAA
GTATGTTTTA
TAATTATTTT
GATCTTGATA
TACTTGGATG
ATGGCATATG
CAGCAGCTAT
ATGTGGATTT
TTTTAGCCCT
GCCTTCATAC
GCTATTTATT
TGCTTGGTAC
TGTTTCTTTT
GTCGATGCTC
ACCCTGTTGT
TTGGTGTTAC
TTCTGCAGGG
CGCCATGGGG
CCGGACATAG
TCATGACACA
AAGTCCGAGT
TCGCTTTCTG
CGAGTGTTGG
GGATAGAGTT
ACGATAACCT
GCAGATCTAG
TACGGGGGCC
GTCACCACGT
CGAATTACGC
GTCATGGGTC
CAAGAAAAGC
CAGGCAAACT
GTTTAAGGGT
CTTATAGGTG
GCACGAACAA
CCGGGCTCCC
GGAGTCCCTT
CCAGGTTTAG
TGGATCACTT
ATTGGTGACA
AGGCAACGCT
GACGATATCT
TCCCTCCAGC
CAGAAGATTT
CGCGACCTAC
TTTTGTGCAC
TTTGGTATTC
TAACCACTGG
GTCTTTGGCC
AAGGGACGAA
GGTCGAGCTC
AAACGGGGTG
GCGGTGGGAG
CGGCGGGGGA
GGATCAGGGG
GCGGAGGTTC
GAGTGGCGGG
GGGAGCGAAG
TCAAACTCCT
CGAGTCCGGT
GGAGGATTGG
TGCAACCAGG
TGGTTCATTG
AAGCTCTCGT
GTGCAGTTTC
TGGTTTCTCT
TTGACAGACT
ACGGTGTCAA
TTGGGTCCGG
CAGGCACCGG
GGCGCGGTCT
TGAGTGGATC
GGCG