名称: | pLV3-H1-2O2-Negative-Control-shRNA-TetR-Puro |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 干扰沉默,四环素诱导 |
片段类型: | shRNA |
片段物种: | 阴性对照 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | H1-2O2 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTGGGGTTGC
GCCTTTTCCA
AGGCAGCCCT
GGGTTTGCGC
AGGGACGCGG
CTGCTCTGGG
CGTGGTTCCG
GGAAACGCAG
CGGCGCCGAC
CCTGGGTCTC
GCACATTCTT
CACGTCCGTT
CGCAGCGTCA
CCCGGATCTT
CGCCGCTACC
CTTGTGGGCC
CCCCGGCGAC
GCTTCCTGCT
CCGCCCCTAA
GTCGGGAAGG
TTCCTTGCGG
TTCGCGGCGT
GCCGGACGTG
ACAAACGGAA
GCCGCACGTC
TCACTAGTAC
CCTCGCAGAC
GGACAGCGCC
AGGGAGCAAT
GGCAGCGCGC
CGACCGCGAT
GGGCTGTGGC
CAATAGCGGC
TGCTCAGCAG
GGCGCGCCGA
GAGCAGCGGC
CGGGAAGGGG
CGGTGCGGGA
GGCGGGGTGT
GGGGCGGTAG
TGTGGGCCCT
GTTCCTGCCC
GCGCGGTGTT
CCGCATTCTG
CAAGCCTCCG
GAGCGCACGT
CGGCAGTCGG
CTCCCTCGTT
GACCGAATCA
CCGACCTCTC
TCCCCAGGGG
GATCTGTGAG
TTTGGGGACC
CTTGATTGTT
CTTTCTTTTT
CGCTATTGTA
AAATTCATGT
TATATGGAGG
GGGCAAAGTT
TTCAGGGTGT
TGTTTAGAAT
GGGAAGATGT
CCCTTGTATC
ACCATGGACC
CTCATGATAA
TTTTGTTTCT
TTCACTTTCT
ACTCTGTTGA
CAACCATTGT
CTCCTCTTAT
TTTCTTTTCA
TTTTCTGTAA
CTTTTTCGTT
AAACTTTAGC
TTGCATTTGT
AACGAATTTT
TAAATTCACT
TTTGTTTATT
TGTCAGATTG
TAAGTACTTT
CTCTAATCAC
TTTTTTTTCA
AGGCAATCAG
GGTATATTAT
ATTGTACTTC
AGCACAGTTT
TAGAGAACAA
TTGTTATAAT
TAAATGATAA
GGTAGAATAT
TTCTGCATAT
AAATTCTGGC
TGGCGTGGAA
ATATTCTTAT
TGGTAGAAAC
AACTACATCC
TGGTCATCAT
CCTGCCTTTC
TCTTTATGGT
TACAATGATA
TACACTGTTT
GAGATGAGGA
TAAAATACTC
TGAGTCCAAA
CCGGGCCCCT
CTGCTAACCA
TGTTCATGCC
TTCTTCTTTT
TCCTACAGCT
CCTGGGCAAC
GTGCTGGTTA
TTGTGCTGTC
TCATCATTTT
GGCAAAGAAT
TGTAATACGA
CTCACTATAG
GGCGAATTGA
TATGTCTAGA
TTAGATAAAA
GTAAAGTGAT
TAACAGCGCA
TTAGAGCTGC
TTAATGAGGT
CGGAATCGAA
GGTTTAACAA
CCCGTAAACT
CGCCCAGAAG
CTAGGTGTAG
AGCAGCCTAC
ATTGTATTGG
CATGTAAAAA
ATAAGCGGGC
TTTGCTCGAC
GCCTTAGCCA
TTGAGATGTT
AGATAGGCAC
CATACTCACT
TTTGCCCTTT
AGAAGGGGAA
AGCTGGCAAG
ATTTTTTACG
TAATAACGCT
AAAAGTTTTA
GATGTGCTTT
ACTAAGTCAT
CGCGATGGAG
CAAAAGTACA
TTTAGGTACA
CGGCCTACAG
AAAAACAGTA
TGAAACTCTC
GAAAATCAAT
TAGCCTTTTT
ATGCCAACAA
GGTTTTTCAC
TAGAGAATGC
ATTATATGCA
CTCAGCGCTG
TGGGGCATTT
TACTTTAGGT
TGCGTATTGG
AAGATCAAGA
GCATCAAGTC
GCTAAAGAAG
AAAGGGAAAC
ACCTACTACT
GATAGTATGC
CGCCATTATT
ACGACAAGCT
ATCGAATTAT
TTGATCACCA
AGGTGCAGAG
CCAGCCTTCT
TATTCGGCCT
TGAATTGATC
ATATGCGGAT
TAGAAAAACA
ACTTAAATGT
GAAAGTGGGT
CCGCGTACAG
CGGCTCCCGG
GAGTTCTAGG
GATCTGCCCC
TCTCCCTCCC
CCCCCCCTAA
CGTTACTGGC
CGAAGCCGCT
TGGAATAAGG
CCGGTGTGCG
TTTGTCTATA
TGTTATTTTC
CACCATATTG
CCGTCTTTTG
GCAATGTGAG
GGCCCGGAAA
CCTGGCCCTG
TCTTCTTGAC
GAGCATTCCT
AGGGGTCTTT
CCCCTCTCGC
CAAAGGAATG
CAAGGTCTGT
TGAATGTCGT
GAAGGAAGCA
GTTCCTCTGG
AAGCTTCTTG
AAGACAAACA
ACGTCTGTAG
CGACCCTTTG
CAGGCAGCGG
AACCCCCCAC
CTGGCGACAG
GTGCCTCTGC
GGCCAAAAGC
CACGTGTATA
AGATACACCT
GCAAAGGCGG
CACAACCCCA
GTGCCACGTT
GTGAGTTGGA
TAGTTGTGGA
AAGAGTCAAA
TGGCTCTCCT
CAAGCGTATT
CAACAAGGGG
CTGAAGGATG
CCCAGAAGGT
ACCCCATTGT
ATGGGATCTG
ATCTGGGGCC
TCGGTGCACA
TGCTTTACAT
GTGTTTAGTC
GAGGTTAAAA
AAACGTCTAG
GCCCCCCGAA
CCACGGGGAC
GTGGTTTTCC
TTTGAAAAAC
ACGATGATAA
GGATCCACCG
GAGCTTACCA
TGACCGAGTA
CAAGCCCACG
GTGCGCCTCG
CCACCCGCGA
CGACGTCCCC
AGGGCCGTAC
GCACCCTCGC
CGCCGCGTTC
GCCGACTACC
CCGCCACGCG
CCACACCGTC
GATCCGGACC
GCCACATCGA
GCGGGTCACC
GAGCTGCAAG
AACTCTTCCT
CACGCGCGTC
GGGCTCGACA
TCGGCAAGGT
GTGGGTCGCG
GACGACGGCG
CCGCGGTGGC
GGTCTGGACC
ACGCCGGAGA
GCGTCGAAGC
GGGGGCGGTG
TTCGCCGAGA
TCGGCCCGCG
CATGGCCGAG
TTGAGCGGTT
CCCGGCTGGC
CGCGCAGCAA
CAGATGGAAG
GCCTCCTGGC
GCCGCACCGG
CCCAAGGAGC
CCGCGTGGTT
CCTGGCCACC
GTCGGCGTCT
CGCCCGACCA
CCAGGGCAAG
GGTCTGGGCA
GCGCCGTCGT
GCTCCCCGGA
GTGGAGGCGG
CCGAGCGCGC
CGGGGTGCCC
GCCTTCCTGG
AGACCTCCGC
GCCCCGCAAC
CTCCCCTTCT
ACGAGCGGCT
CGGCTTCACC
GTCACCGCCG
ACGTCGAGGT
GCCCGAAGGA
CCGCGCACCT
GGTGCATGAC
CCGCAAGCCC
GGTGCCTGAC
GCCCGCCCCA
CGACCCGCAG
CGCCCGACCG
AAAGGAGCGC
ACGACCCCAT
GCATCGGTAC
CTTTAAGACC
AATGACTTAC
AAGGCAGCTG
TAGATCTTAG
CCACTTTTTA
AAAGAAAAGG
GGGGACTGGA
AGGGCTAATT
CACTCCCAAC
GAAGACAAGA
TCTGCTTTTT
GCTTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTAGTAGTTC
ATGTCATCTT
ATTATTCAGT
ATTTATAACT
TGCAAAGAAA
TGAATATCAG
AGAGTGAGAG
GAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGGCTCTAGC
TATCCCGCCC
CTAACTCCGC
CCATCCCGCC
CCTAACTCCG
CCCAGTTCCG
CCCATTCTCC
GCCCCATGGC
TGACTAATTT
TTTTTATTTA
TGCAGAGGCC
GAGGCCGCCT
CGGCCTCTGA
GCTATTCCAG
AAGTAGTGAG
GAGGCTTTTT
TGGAGGCCTA
GGGACGTACC
CAATTCGCCC
TATAGTGAGT
CGTATTACGC
GCGCTCACTG
GCCGTCGTTT
TACAACGTCG
TGACTGGGAA
AACCCTGGCG
TTACCCAACT
TAATCGCCTT
GCAGCACATC
CCCCTTTCGC
CAGCTGGCGT
AATAGCGAAG
AGGCCCGCAC
CGATCGCCCT
TCCCAACAGT
TGCGCAGCCT
GAATGGCGAA
TGGGACGCGC
CCTGTAGCGG
CGCATTAAGC
GCGGCGGGTG
TGGTGGTTAC
GCGCAGCGTG
ACCGCTACAC
TTGCCAGCGC
CCTAGCGCCC
GCTCCTTTCG
CTTTCTTCCC
TTCCTTTCTC
GCCACGTTCG
CCGGCTTTCC
CCGTCAAGCT
CTAAATCGGG
GGCTCCCTTT
AGGGTTCCGA
TTTAGTGCTT
TACGGCACCT
CGACCCCAAA
AAACTTGATT
AGGGTGATGG
TTCACGTAGT
GGGCCATCGC
CCTGATAGAC
GGTTTTTCGC
CCTTTGACGT
TGGAGTCCAC
GTTCTTTAAT
AGTGGACTCT
TGTTCCAAAC
TGGAACAACA
CTCAACCCTA
TCTCGGTCTA
TTCTTTTGAT
TTATAAGGGA
TTTTGCCGAT
TTCGGCCTAT
TGGTTAAAAA
ATGAGCTGAT
TTAACAAAAA
TTTAACGCGA
ATTTTAACAA
AATATTAACG
CTTACAATTT
AGGTGGCACT
TTTCGGGGAA
ATGTGCGCGG
AACCCCTATT
TGTTTATTTT
TCTAAATACA
TTCAAATATG
TATCCGCTCA
TGAGACAATA
ACCCTGATAA
ATGCTTCAAT
AATATTGAAA
AAGGAAGAGT
ATGAGTATTC
AACATTTCCG
TGTCGCCCTT
ATTCCCTTTT
TTGCGGCATT
TTGCCTTCCT
GTTTTTGCTC
ACCCAGAAAC
GCTGGTGAAA
GTAAAAGATG
CTGAAGATCA
GTTGGGTGCA
CGAGTGGGTT
ACATCGAACT
GGATCTCAAC
AGCGGTAAGA
TCCTTGAGAG
TTTTCGCCCC
GAAGAACGTT
TTCCAATGAT
GAGCACTTTT
AAAGTTCTGC
TATGTGGCGC
GGTATTATCC
CGTATTGACG
CCGGGCAAGA
GCAACTCGGT
CGCCGCATAC
ACTATTCTCA
GAATGACTTG
GTTGAGTACT
CACCAGTCAC
AGAAAAGCAT
CTTACGGATG
GCATGACAGT
AAGAGAATTA
TGCAGTGCTG
CCATAACCAT
GAGTGATAAC
ACTGCGGCCA
ACTTACTTCT
GACAACGATC
GGAGGACCGA
AGGAGCTAAC
CGCTTTTTTG
CACAACATGG
GGGATCATGT
AACTCGCCTT
GATCGTTGGG
AACCGGAGCT
GAATGAAGCC
ATACCAAACG
ACGAGCGTGA
CACCACGATG
CCTGTAGCAA
TGGCAACAAC
GTTGCGCAAA
CTATTAACTG
GCGAACTACT
TACTCTAGCT
TCCCGGCAAC
AATTAATAGA
CTGGATGGAG
GCGGATAAAG
TTGCAGGACC
ACTTCTGCGC
TCGGCCCTTC
CGGCTGGCTG
GTTTATTGCT
GATAAATCTG
GAGCCGGTGA
GCGTGGGTCT
CGCGGTATCA
TTGCAGCACT
GGGGCCAGAT
GGTAAGCCCT
CCCGTATCGT
AGTTATCTAC
ACGACGGGGA
GTCAGGCAAC
TATGGATGAA
CGAAATAGAC
AGATCGCTGA
GATAGGTGCC
TCACTGATTA
AGCATTGGTA
ACTGTCAGAC
CAAGTTTACT
CATATATACT
TTAGATTGAT
TTAAAACTTC
ATTTTTAATT
TAAAAGGATC
TAGGTGAAGA
TCCTTTTTGA
TAATCTCATG
ACCAAAATCC
CTTAACGTGA
GTTTTCGTTC
CACTGAGCGT
CAGACCCCGT
AGAAAAGATC
AAAGGATCTT
CTTGAGATCC
TTTTTTTCTG
CGCGTAATCT
GCTGCTTGCA
AACAAAAAAA
CCACCGCTAC
CAGCGGTGGT
TTGTTTGCCG
GATCAAGAGC
TACCAACTCT
TTTTCCGAAG
GTAACTGGCT
TCAGCAGAGC
GCAGATACCA
AATACTGTTC
TTCTAGTGTA
GCCGTAGTTA
GGCCACCACT
TCAAGAACTC
TGTAGCACCG
CCTACATACC
TCGCTCTGCT
AATCCTGTTA
CCAGTGGCTG
CTGCCAGTGG
CGATAAGTCG
TGTCTTACCG
GGTTGGACTC
AAGACGATAG
TTACCGGATA
AGGCGCAGCG
GTCGGGCTGA
ACGGGGGGTT
CGTGCACACA
GCCCAGCTTG
GAGCGAACGA
CCTACACCGA
ACTGAGATAC
CTACAGCGTG
AGCTATGAGA
AAGCGCCACG
CTTCCCGAAG
GGAGAAAGGC
GGACAGGTAT
CCGGTAAGCG
GCAGGGTCGG
AACAGGAGAG
CGCACGAGGG
AGCTTCCAGG
GGGAAACGCC
TGGTATCTTT
ATAGTCCTGT
CGGGTTTCGC
CACCTCTGAC
TTGAGCGTCG
ATTTTTGTGA
TGCTCGTCAG
GGGGGCGGAG
CCTATGGAAA
AACGCCAGCA
ACGCGGCCTT
TTTACGGTTC
CTGGCCTTTT
GCTGGCCTTT
TGCTCACATG
TTCTTTCCTG
CGTTATCCCC
TGATTCTGTG
GATAACCGTA
TTACCGCCTT
TGAGTGAGCT
GATACCGCTC
GCCGCAGCCG
AACGACCGAG
CGCAGCGAGT
CAGTGAGCGA
GGAAGCGGAA
GAGCGCCCAA
TACGCAAACC
GCCTCTCCCC
GCGCGTTGGC
CGATTCATTA
ATGCAGCTGG
CACGACAGGT
TTCCCGACTG
GAAAGCGGGC
AGTGAGCGCA
ACGCAATTAA
TGTGAGTTAG
CTCACTCATT
AGGCACCCCA
GGCTTTACAC
TTTATGCTTC
CGGCTCGTAT
GTTGTGTGGA
ATTGTGAGCG
GATAACAATT
TCACACAGGA
AACAGCTATG
ACCATGATTA
CGCCAAGCGC
GCAATTAACC
CTCACTAAAG
GGAACAAAAG
CTGGAGCTGC
AAGCTTAATG
TAGTCTTATG
CAATACTCTT
GTAGTCTTGC
AACATGGTAA
CGATGAGTTA
GCAACATGCC
TTACAAGGAG
AGAAAAAGCA
CCGTGCATGC
CGATTGGTGG
AAGTAAGGTG
GTACGATCGT
GCCTTATTAG
GAAGGCAACA
GACGGGTCTG
ACATGGATTG
GACGAACCAC
TGAATTGCCG
CATTGCAGAG
ATATTGTATT
TAAGTGCCTA
GCTCGATACA
TAAACGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTGG
CGCCCGAACA
GGGACTTGAA
AGCGAAAGGG
AAACCAGAGG
AGCTCTCTCG
ACGCAGGACT
CGGCTTGCTG
AAGCGCGCAC
GGCAAGAGGC
GAGGGGCGGC
GACTGGTGAG
TACGCCAAAA
ATTTTGACTA
GCGGAGGCTA
GAAGGAGAGA
GATGGGTGCG
AGAGCGTCAG
TATTAAGCGG
GGGAGAATTA
GATCGCGATG
GGAAAAAATT
CGGTTAAGGC
CAGGGGGAAA
GAAAAAATAT
AAATTAAAAC
ATATAGTATG
GGCAAGCAGG
GAGCTAGAAC
GATTCGCAGT
TAATCCTGGC
CTGTTAGAAA
CATCAGAAGG
CTGTAGACAA
ATACTGGGAC
AGCTACAACC
ATCCCTTCAG
ACAGGATCAG
AAGAACTTAG
ATCATTATAT
AATACAGTAG
CAACCCTCTA
TTGTGTGCAT
CAAAGGATAG
AGATAAAAGA
CACCAAGGAA
GCTTTAGACA
AGATAGAGGA
AGAGCAAAAC
AAAAGTAAGA
CCACCGCACA
GCAAGCGGCC
GCTGATCTTC
AGACCTGGAG
GAGGAGATAT
GAGGGACAAT
TGGAGAAGTG
AATTATATAA
ATATAAAGTA
GTAAAAATTG
AACCATTAGG
AGTAGCACCC
ACCAAGGCAA
AGAGAAGAGT
GGTGCAGAGA
GAAAAAAGAG
CAGTGGGAAT
AGGAGCTTTG
TTCCTTGGGT
TCTTGGGAGC
AGCAGGAAGC
ACTATGGGCG
CAGCGTCAAT
GACGCTGACG
GTACAGGCCA
GACAATTATT
GTCTGGTATA
GTGCAGCAGC
AGAACAATTT
GCTGAGGGCT
ATTGAGGCGC
AACAGCATCT
GTTGCAACTC
ACAGTCTGGG
GCATCAAGCA
GCTCCAGGCA
AGAATCCTGG
CTGTGGAAAG
ATACCTAAAG
GATCAACAGC
TCCTGGGGAT
TTGGGGTTGC
TCTGGAAAAC
TCATTTGCAC
CACTGCTGTG
CCTTGGAATG
CTAGTTGGAG
TAATAAATCT
CTGGAACAGA
TTTGGAATCA
CACGACCTGG
ATGGAGTGGG
ACAGAGAAAT
TAACAATTAC
ACAAGCTTAA
TACACTCCTT
AATTGAAGAA
TCGCAAAACC
AGCAAGAAAA
GAATGAACAA
GAATTATTGG
AATTAGATAA
ATGGGCAAGT
TTGTGGAATT
GGTTTAACAT
AACAAATTGG
CTGTGGTATA
TAAAATTATT
CATAATGATA
GTAGGAGGCT
TGGTAGGTTT
AAGAATAGTT
TTTGCTGTAC
TTTCTATAGT
GAATAGAGTT
AGGCAGGGAT
ATTCACCATT
ATCGTTTCAG
ACCCACCTCC
CAACCCCGAG
GGGACCCGAC
AGGCCCGAAG
GAATAGAAGA
AGAAGGTGGA
GAGAGAGACA
GAGACAGATC
CATTCGATTA
GTGAACGGAT
CTCGACGGTA
TCGATCACGA
GACTAGCCTC
GAGCGGCCGC
AATATTTGCA
TGTCGCTATG
TGTTCTGGGA
AATCACCATA
AACGTGAAAT
CCCTATCAGT
GATAGAGACT
TATAAGTTCC
CTATCAGTGA
TAGAGACACC
GGCAACAAGA
TGAAGAGCAC
CAATTCAAGA
GATTGGTGCT
CTTCATCTTG
TTGTTTTTTG
AATTCTCGAC
CTCGAGACAA
ATGGCAGTAT
TCATCCACAA
TTTTAAAAGA
AAAGGGGGGA
TTGGGGGGTA
CAGTGCAGGG
GAAAGAATAG
TAGACATAAT
AGCAACAGAC
ATACAAACTA
AAGAATTACA
AAAACAAATT
ACAAAAATTC
AAAATTTTCG
GGTTTATTAC
AGGGACAGCA
GAGATCCACT
TTGGCCGCGG
CTCGAGGGGG