名称: | pSCBE3 |
---|---|
别称: | Plasmid#133966 |
基因长: | |
质粒宿主: | 链霉菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AGCTCGGCTT
TTCGCCATTC
GTATTGCACG
ACATTGCACT
CCACCGCTGA
TGACATCAGT
CGATCATAGC
ACGATCAACG
GCACTGTTGC
AAATAGTCGG
TGGTGATAAA
CTTATCATCC
CCTTTTGCTG
ATGGAGCTGC
ACATGAACCC
ATTCAAAGGC
CGGCATTTTC
AGCGTGACAT
CATTCTGTGG
GCCGTACGCT
GGTACTGCAA
ATACGGCATC
AGTTACCGTG
AGCTGCAGGT
CGACGGATCC
CTTTAGTGAG
GGTTAATTGC
GGCCGCGAAT
TCTTGAAGAC
GAAAGGGCCT
CGTGATACGC
CTATTTTTAT
AGGTTAATGT
CATGATAATA
ATGGTTTCTT
AGACGTCAGG
TGGCACTTTT
CGGGGAAATG
TGCGCGGAAC
CCCTATTTGT
TTATTTTTCT
AAATACATTC
AAATATGTAT
CCGCTCATGA
GACAATAACC
CTGATAAATG
CTTCAATAAT
ATTGAAAAAG
GAAGAGTATG
AGTATTCAAC
ATTTCCGTGT
CGCCCTTATT
CCCTTTTTTG
CGGCATTTTG
CCTTCCTGTT
TTTGCTCACC
CAGAAACGCT
GGTGAAAGTA
AAAGATGCTG
AAGATCAGTT
GGGTGCACGA
GTGGGTTACA
TCGAACTGGA
TCTCAACAGC
GGTAAGATCC
TTGAGAGTTT
TCGCCCCGAA
GAACGTTTTC
CAATGATGAG
CACTTTTAAA
GTTCTGCTAT
GTGGCGCGGT
ATTATCCCGT
GTTGACGCCG
GGCAAGAGCA
ACTCGGTCGC
CGCATACACT
ATTCTCAGAA
TGACTTGGTT
GAGTACTCAC
CAGTCACAGA
AAAGCATCTT
ACGGATGGCA
TGACAGTAAG
AGAATTATGC
AGTGCTGCCA
TAACCATGAG
TGATAACACT
GCGGCCAACT
TACTTCTGAC
AACGATCGGA
GGACCGAAGG
AGCTAACCGC
TTTTTTGCAC
AACATGGGGG
ATCATGTAAC
TCGCCTTGAT
CGTTGGGAAC
CGGAGCTGAA
TGAAGCCATA
CCAAACGACG
AGCGTGACAC
CACGATGCCT
GCAGCAATGG
CAACAACGTT
GCGCAAACTA
TTAACTGGCG
AACTACTTAC
TCTAGCTTCC
CGGCAACAAT
TAATAGACTG
GATGGAGGCG
GATAAAGTTG
CAGGACCACT
TCTGCGCTCG
GCCCTTCCGG
CTGGCTGGTT
TATTGCTGAT
AAATCTGGAG
CCGGTGAGCG
TGGGTCTCGC
GGTATCATTG
CAGCACTGGG
GCCAGATGGT
AAGCCCTCCC
GTATCGTAGT
TATCTACACG
ACGGGGAGTC
AGGCAACTAT
GGATGAACGA
AATAGACAGA
TCGCTGAGAT
AGGTGCCTCA
CTGATTAAGC
ATTGGTAACT
GTCAGACCAA
GTTTACTCAT
ATATACTTTA
GATTGATTTA
AAACTTCATT
TTTAATTTAA
AAGGATCTAG
GTGAAGATCC
TTTTTGATAA
TCTCATGACC
AAAATCCCTT
AACGTGAGTT
TTCGTTCCAC
TGAGCGTCAG
ACCCCGTAGA
AAAGATCAAA
GGATCTTCTT
GAGATCCTTT
TTTTCTGCGC
GTAATCTGCT
GCTTGCAAAC
AAAAAAACCA
CCGCTACCAG
CGGTGGTTTG
TTTGCCGGAT
CAAGAGCTAC
CAACTCTTTT
TCCGAAGGTA
ACTGGCTTCA
GCAGAGCGCA
GATACCAAAT
ACTGTCCTTC
TAGTGTAGCC
GTAGTTAGGC
CACCACTTCA
AGAACTCTGT
AGCACCGCCT
ACATACCTCG
CTCTGCTAAT
CCTGTTACCA
GTGGCTGCTG
CCAGTGGCGA
TAAGTCGTGT
CTTACCGGGT
TGGACTCAAG
ACGATAGTTA
CCGGATAAGG
CGCAGCGGTC
GGGCTGAACG
GGGGGTTCGT
GCACACAGCC
CAGCTTGGAG
CGAACGACCT
ACACCGAACT
GAGATACCTA
CAGCGTGAGC
TATGAGAAAG
CGCCACGCTT
CCCGAAGGGA
GAAAGGCGGA
CAGGTATCCG
GTAAGCGGCA
GGGTCGGAAC
AGGAGAGCGC
ACGAGGGAGC
TTCCAGGGGG
AAACGCCTGG
TATCTTTATA
GTCCTGTCGG
GTTTCGCCAC
CTCTGACTTG
AGCGTCGATT
TTTGTGATGC
TCGTCAGGGG
GGCGGAGCCT
ATGGAAAAAC
GCCAGCAACG
CGGCCTTTTT
ACGGTTCCTG
GCCTTTTGCT
GGCCTTTTGC
TCACATGTTC
TTTCCTGCGT
TATCCCCTGA
TTCTGTGGAT
AACCGTATTA
CCGCCTTTGA
GTGAGCTGAT
ACCGCTCGCC
GCAGCCGAAC
GACCGAGCGC
AGCGAGTCAG
TGAGCGAGGA
AGCGGAAGAG
CGCTGACTTC
CGCGTTTCCA
GACTTTACGA
AACACGGAAA
CCGAAGACCA
TTCATGTTGT
TGCTCAGGTC
GCAGACGTTT
TGCAGCAGCA
GTCGCTTCAC
GTTCGCTCGC
GTATCGGTGA
TTCATTCTGC
TAACCAGTAA
GGCAACCCCG
CCAGCCTAGC
CGGGTCCTCA
ACGACAGGAG
CACGATCATG
CGCACCCGTC
AGATCCAGAC
ATGATAAGAT
ACATTGATGA
GTTTGGACAA
ACCACAACTA
GAATGCAGTG
AAAAAAATGC
TTTATTTGTG
AAATTTGTGA
TGCTATTGCT
TTATTTGTAA
CCATTATAAG
CTGCAATAAA
CAAGTTCCCG
CACGATCAGC
GTCCCGCCGA
CCAGGAGCAG
TGCGGCCAGC
ACTGCCGCTA
ACGCCTGGTC
CTGGTCCCGG
TCCTGGTGGT
GCATCAGTCC
TCCCCGTGAT
CACTTCGGCA
CCCACCGTAG
TGATCACCCC
CGACAGCGGA
TCAAGGGGTT
TGCGGGTCCC
GGTCGGCGCC
GGGCGGGGGA
GGCAGGAGCC
GCCGACGCTG
CCTCTGGGAC
GGGCCGGACG
GCAGGGGGAC
CGGCGGCCGG
GCGAGCTGCA
GCCGGGGGTC
CGGCAGGGCC
GGAGCGGGCG
GAACCGTGCT
CTGACCTGCG
GCCCGAGTTT
CGTCACGTGA
CGGAATGGAA
GGCTGCTGCA
TTTCGTCACG
TGACGTATCT
CGGCGAGCGA
CTGCCGACGC
CACGGCGGAC
ACGATCGCCT
CGCGCTGGCG
CCGGGCCTCG
TACGCCCGCT
GGCGGCAGGA
GCGGCGGCAG
TAGTCCCGGC
TCCGGCCGAC
GCCGGATTGC
TTGATCTCCG
AGCCGCACCA
GGCGCAGAGC
TTCGCGCCGT
CGGCGTCCCT
GGGGGTGGTG
GTGCTCATGG
CCGACGACCG
TACGCGGCAC
GTCTCGTAGC
GAGGCGAGTC
GGGCGCGAGG
TACCGCCTGC
ACGAAGTGCC
GGCGGGGCCG
ACCCCGGGCG
AGTAATCCCA
GGATTACTCC
CGCGGCTTCG
ACCCCGGCCG
CCGTCGCCGC
GTACGTCACC
GACCCCCGCC
GTACGTCACC
GGGATGACGT
ACGGCGGGGG
GGAGCGAGTT
AGTGCGAAGT
GGGCCCACTT
GCGAGCCGGG
CGATGTGCCG
GGCGGCCCGC
TCCTGGCGGT
CGTCGGCGTC
GTCGTCCTGG
TCGTCGTCCT
GCTCTCGCCG
TCGGCGTGCA
GTTGCTTCCT
CGCGGCGCTG
GGCGAGGGCG
GCGAGCATGT
CGGCGTACGC
CTCGGCCACC
TCCCCCGCCG
TGAGCACCAC
CACTGTGTCG
GCCGCGTCGG
CCAGCGCCAG
GACCTCCCGC
ACCCGTTCGC
CCACGGCCGC
CGAATCCTCG
TTGCCGTCCT
TGCCTTCGGC
GGCCCGGGTC
GCCTCGAGGT
CGAGGGCGCG
GCGGGTGACC
GCGTGCCATC
CGTCCTCGGT
CACGGCGACC
CCGGCCCGCA
GCTCCCCGCC
GTCGGCGTCG
GCCGCCAGGA
GCAGATCGAG
GTCGTCGGCC
TCGGTGTCGC
CGCCGTCGAG
CCCGAGCATC
TGCCGCAGGT
AGCGGGTCCA
TTCGATGGCC
CGGCGTCCCC
GGGTTGCCCG
CTCGTACTCG
TGCCAGCGCG
AGAGGTTCCA
CTCCAGCGAG
CCGACCCCGG
CGGCGTCGTC
CTCGGTCATG
CCGCCGGTCA
GGTCCCCGAT
CCGTCCGAGG
AGTTCGAACG
GGGCGACGTT
CCCGCCGGTC
GCCGTCTTGA
GGTCGGCGCG
GGCGAGTTCG
AGGGCGGGCG
CCTTCCCGTC
CTGGGTCTTG
GCGATGTACT
CGGCGAGGTC
GTTGGCGTCG
CGCTCGGTCT
CCAGCCGCTT
GAAGTCGACG
CCGTGCCGGT
CGTCGGGCGT
GAAGGCGGGG
TTGACCTTGC
GCAGGGCGGC
GGTCCACACG
GACCGCCAGT
GCCCCTGCCA
CTCGTCGAGC
GCGGCGCCGG
TCGGCTCGAA
GGTGGCGACG
ATCTGCTTCG
CGGACCGCTC
CCCCTCGGTC
CGGCCGCCGA
CCAGGACGAT
CGCGTGGATG
TGCGGGTGCC
AGCCGTTGAT
CTGCCCCACG
GTGACTTCGG
TCGCGCGGAT
CATGCCGACG
TACCCGATCC
GGTCTCGGAT
GCCCTCGCGG
TCGGCGGCCC
GGTGCCCGTC
CTTGGCCCGG
CGTCCGGCCC
ACGTGCCGCC
CGTGATCAGT
CGCTGGTAGG
CGCCCGGCCG
CCGGGGGCTG
TCCGGCGTCT
TCCGGGTGCC
CTGGAGGGCG
TCCATGAGGT
CCGCGAGCCG
GTCCGTGTGC
CCATGGCGGG
CCGTGAAGGT
GACCAGGTAG
GCGGTCCCCC
CGCGCTTGAT
CCACTCGACC
ACGGCGGCGG
TGATCTCCTC
GGCCCGCTTG
TGCCGGATCG
TGGCGGCGCA
GACCGGGCAG
AGCCAGATCC
GCCCGCACCG
CATCAGGCCC
AGGACCACGG
ACGTTCCGGC
CGCCGTCTGG
GCGACGATCA
CGCCGGAGGC
AGGGTCCATC
AGGGCGCGGC
CGCAGCCCTT
GCACGCGGCG
TCCCCGCTGA
TCCGCCACAG
CGTCCGGCGG
CGGCTGTACC
GGGCGGCTTT
CCGCAGTCGG
GCAGCGTCGC
TCCGCGACGT
GCTTCCTACT
TCCGAGAGGC
TGTCGCCTCT
CGGGCTCTCC
CCATCCACCC
CGTCCGGAGA
AACCGCAGGT
CGGAGGGGTG
CGGGAAACTC
TGTTGTTTCT
TTCCCAAGGT
GTTCGCTTTT
GCCTCGGGCG
GCATCTCGCG
TCACACGCGC
GATCGCCCGC
TTCGCTGCCA
TCCGGCAGCG
GTCTGAGCAG
TAGATACGCG
GCCGTTTGCC
CGGTGTGTGG
GCAATTGCGG
TCCCGCAGTG
GCAGCGGGGC
CCGGCGGGCC
GATCTGGCAA
TGCCTCGGCA
TCGCTCCGTA
CTCTGGGCAC
GAGCAACGTT
CCTGTCTCGC
CCGGCTAAGG
GGCGCGAGTC
TGGGAGCGGA
CGGGTCGGAG
GTGCGAAGTC
CGGCCCGTTG
CTCTTTGGTC
TGGTGGGAAT
CCTGGCACCA
ATCGGGCCAG
AGGTTCCCTC
CGCCACTCCC
GACGCCCCTT
GGGGCTGGTG
TGACTTGGAG
GGCCGAAGAG
AGCCCCGCCG
GTGATCCGGC
GGGGCTTTGA
CGTGCGGTCA
GTGCGTGTGT
CGGCGAGCGA
TGGCCACGAG
GCCCTGGAAG
CCGAGCGGTC
CGGCGAAGTC
GGCCCAGTCG
CAACCGGGCT
CAGCGCAGTG
GGCGGACCAG
CCACCGCCGT
TGGGGTCCTG
GACCAGGTTC
ACGGTCCCCT
CGGTCAGGCG
TCCGTCGAAG
TCGGTCATGG
TCGGTCTCCT
GGTGGGTGGG
GGCGGGGCGC
CAGCACGAAG
TGCCGGCGCC
CCGCGGGGGT
TGGTCGGGTC
AGGCGCCGAA
CCGGCGGGCG
GCGGCGGCGA
CCAGGCCGTC
GGCGGCGGCC
ATGGCGCGGT
CGCGGTCGGT
GGTGAGGGCG
GTGCGGTCGG
CGGCGGCCCA
GTCCTGCTCT
CACGCAACGT
CTACGTGGAC
GCTCAGGGCG
ACACGATCGA
GGTCGGGGAG
TCCGTCGCTC
CGGCGGGCCG
CTGGCGCGTC
CACCGGGACT
GATCAAGGCG
AATACTTCAT
ATGGCCCTGC
AGGCGGAAGT
CAGGTAGACA
CGACTTCCGC
TAGTCCTTGC
AAGGTCTGCT
GACGTGAGGC
GGGGCGGTCG
TTTTTGACCG
CCCTGCCTTC
GTCATGTAGG
CTCGCTCGCT
GTGCCTGGCG
TGTCATCAGA
CGCCCAGGTC
CCGGTGCCGT
GAGGCCCGGG
CCATCGAGCC
GGTGGTACGT
GGCTGCGGTC
CCCTTGTGAG
GGCTGCGCGC
CGTGTGCTGT
CCGGCGCGCA
CAGCCTTGAA
TCCACCCGCG
GGGGCCGGCC
GGTCTCCGTG
AGCTCGAGTA
GACGACGGAG
ACGTAATGTC
GTCCGAGACC
GGCCCGGTGG
CCGTGGACCC
GACCCTGCGT
CGCCGCATCG
AGCCGCACGA
GTTCGAGGTG
TTCTTCGACC
CGCGGGAGCT
GCGCAAGGAG
ACCTGCCTGC
TGTACGAGAT
CAACTGGGGC
GGCCGGCACT
CGATCTGGCG
CCACACCTCG
CAGAACACCA
ACAAGCACGT
CGAGGTGAAC
TTCATCGAGA
AGTTCACCAC
CGAGCGGTAC
TTCTGCCCGA
ACACCCGCTG
CTCGATCACC
TGGTTCCTGT
CCTGGTCCCC
GTGCGGCGAG
TGCTCCCGGG
CGATCACCGA
GTTCCTGTCC
CGCTACCCGC
ACGTCACCCT
GTTCATCTAC
ATCGCCCGGC
TGTACCACCA
CGCCGACCCG
CGGAACCGGC
AGGGCCTGCG
CGACCTGATC
TCCTCCGGCG
TGACCATCCA
GATCATGACC
GAGCAGGAGT
CCGGCTACTG
CTGGCGGAAC
TTCGTCAACT
ACTCGCCGTC
CAACGAGGCC
CACTGGCCGC
GGTACCCGCA
CCTGTGGGTG
CGCCTGTACG
TCCTGGAGCT
GTACTGCATC
ATCCTGGGCC
TGCCGCCGTG
CCTGAACATC
CTGCGTCGCA
AGCAGCCGCA
GCTGACCTTC
TTCACCATCG
CCCTGCAGTC
CTGCCACTAC
CAGCGCCTGC
CGCCGCACAT
CCTGTGGGCG
ACCGGCCTGA
AGTCCGGCTC
GGAGACCCCG
GGCACCTCGG
AGTCCGCCAC
CCCGGAGTCC
GACAAGAAGT
ACAGCATCGG
CCTGGCCATC
GGCACCAACA
GCGTGGGCTG
GGCGGTCATC
ACCGACGAGT
ACAAGGTCCC
CTCCAAGAAG
TTCAAGGTCC
TGGGCAACAC
CGACCGGCAC
TCGATCAAGA
AGAACCTGAT
CGGCGCCCTG
CTCTTCGACA
GCGGCGAGAC
CGCCGAGGCG
ACCCGCCTGA
AGCGGACCGC
GCGCCGGCGC
TACACCCGGC
GCAAGAACCG
CATCTGCTAC
CTCCAGGAAA
TCTTCTCCAA
CGAGATGGCC
AAGGTCGACG
ACTCGTTCTT
CCACCGGCTC
GAGGAGAGCT
TCCTGGTGGA
GGAGGACAAG
AAGCACGAGC
GCCACCCGAT
CTTCGGCAAC
ATCGTCGACG
AGGTGGCCTA
CCACGAGAAG
TACCCCACCA
TCTACCACCT
CCGCAAGAAG
CTGGTCGACT
CGACCGACAA
GGCGGACCTG
CGGCTCATCT
ACCTGGCCCT
CGCGCACATG
ATCAAGTTCC
GCGGCCACTT
CCTCATCGAG
GGCGACCTGA
ACCCGGACAA
CTCCGACGTC
GACAAGCTCT
TCATCCAGCT
GGTGCAGACC
TACAACCAGC
TGTTCGAGGA
GAACCCCATC
AACGCCAGCG
GCGTCGACGC
CAAGGCGATC
CTCTCCGCGC
GCCTGAGCAA
GTCCCGGCGC
CTGGAGAACC
TCATCGCCCA
GCTGCCGGGC
GAGAAGAAGA
ACGGCCTCTT
CGGCAACCTG
ATCGCGCTGT
CGCTCGGCCT
GACCCCCAAC
TTCAAGAGCA
ACTTCGACCT
GGCCGAGGAC
GCGAAGCTCC
AGCTGTCCAA
GGACACCTAC
GACGACGACC
TGGACAACCT
GCTCGCCCAG
ATCGGCGACC
AGTACGCGGA
CCTCTTCCTG
GCCGCGAAGA
ACCTCTCGGA
CGCCATCCTG
CTCAGCGACA
TCCTGCGGGT
CAACACCGAG
ATCACCAAGG
CCCCGCTGTC
GGCGAGCATG
ATCAAGCGGT
ACGACGAGCA
CCACCAGGAC
CTGACCCTGC
TCAAGGCCCT
CGTGCGCCAG
CAGCTGCCCG
AGAAGTACAA
GGAAATCTTC
TTCGACCAGT
CCAAGAACGG
CTACGCCGGC
TACATCGACG
GCGGCGCGTC
GCAGGAGGAG
TTCTACAAGT
TCATCAAGCC
GATCCTGGAG
AAGATGGACG
GCACCGAGGA
GCTGCTCGTC
AAGCTGAACC
GCGAGGACCT
GCTCCGCAAG
CAGCGGACCT
TCGACAACGG
CTCCATCCCG
CACCAGATCC
ACCTGGGCGA
GCTGCACGCC
ATCCTCCGGC
GCCAGGAGGA
CTTCTACCCC
TTCCTGAAGG
ACAACCGCGA
GAAGATCGAG
AAGATCCTGA
CCTTCCGCAT
CCCGTACTAC
GTCGGCCCCC
TGGCCCGCGG
CAACTCCCGG
TTCGCGTGGA
TGACCCGGAA
GTCGGAGGAG
ACCATCACCC
CGTGGAACTT
CGAGGAGGTC
GTGGACAAGG
GCGCCTCCGC
CCAGTCGTTC
ATCGAGCGCA
TGACCAACTT
CGACAAGAAC
CTCCCGAACG
AGAAGGTCCT
GCCCAAGCAC
TCCCTGCTCT
ACGAGTACTT
CACCGTGTAC
AACGAGCTGA
CCAAGGTCAA
GTACGTGACC
GAGGGCATGC
GGAAGCCGGC
CTTCCTGTCG
GGCGAGCAGA
AGAAGGCGAT
CGTCGACCTG
CTCTTCAAGA
CCAACCGCAA
GGTCACCGTG
AAGCAGCTGA
AGGAGGACTA
CTTCAAGAAG
ATCGAGTGCT
TCGACTCCGT
CGAGATCAGC
GGCGTGGAGG
ACCGCTTCAA
CGCCTCCCTG
GGCACCTACC
ACGACCTGCT
CAAGATCATC
AAGGACAAGG
ACTTCCTCGA
CAACGAGGAG
AACGAGGACA
TCCTGGAGGA
CATCGTCCTC
ACCCTGACCC
TCTTCGAGGA
CCGCGAGATG
ATCGAGGAGC
GGCTCAAGAC
CTACGCCCAC
CTGTTCGACG
ACAAGGTGAT
GAAGCAGCTG
AAGCGGCGCC
GGTACACCGG
CTGGGGCCGC
CTCTCCCGGA
AGCTGATCAA
CGGCATCCGG
GACAAGCAGA
GCGGCAAGAC
CATCCTGGAC
TTCCTCAAGT
CCGACGGCTT
CGCCAACCGC
AACTTCATGC
AGCTCATCCA
CGACGACAGC
CTGACCTTCA
AGGAGGACAT
CCAGAAGGCC
CAGGTCTCGG
GCCAGGGCGA
CAGCCTCCAC
GAGCACATCG
CCAACCTGGC
GGGCTCCCCG
GCGATCAAGA
AGGGCATCCT
CCAGACCGTC
AAGGTCGTGG
ACGAGCTGGT
CAAGGTGATG
GGCCGCCACA
AGCCCGAGAA
CATCGTGATC
GAGATGGCCC
GGGAGAACCA
GACCACCCAG
AAGGGCCAGA
AGAACTCGCG
CGAGCGGATG
AAGCGGATCG
AGGAGGGCAT
CAAGGAGCTG
GGCAGCCAGA
TCCTGAAGGA
GCACCCGGTC
GAGAACACCC
AGCTCCAGAA
CGAGAAGCTG
TACCTCTACT
ACCTCCAGAA
CGGCCGCGAC
ATGTACGTGG
ACCAGGAGCT
GGACATCAAC
CGGCTGTCCG
ACTACGACGT
CGACCACATC
GTGCCGCAGT
CCTTCCTGAA
GGACGACTCG
ATCGACAACA
AGGTCCTGAC
CCGCTCGGAC
AAGAACCGGG
GCAAGTCCGA
CAACGTGCCC
TCGGAGGAGG
TCGTGAAGAA
GATGAAGAAC
TACTGGCGCC
AGCTGCTCAA
CGCCAAGCTC
ATCACCCAGC
GCAAGTTCGA
CAACCTGACC
AAGGCCGAGC
GGGGCGGCCT
GAGCGAGCTG
GACAAGGCGG
GCTTCATCAA
GCGCCAGCTG
GTCGAGACCC
GGCAGATCAC
CAAGCACGTG
GCCCAGATCC
TGGACTCCCG
GATGAACACC
AAGTACGACG
AGAACGACAA
GCTGATCCGC
GAGGTCAAGG
TGATCACCCT
CAAGAGCAAG
CTGGTCTCCG
ACTTCCGCAA
GGACTTCCAG
TTCTACAAGG
TCCGGGAGAT
CAACAACTAC
CACCACGCCC
ACGACGCGTA
CCTGAACGCC
GTCGTGGGCA
CCGCGCTGAT
CAAGAAGTAC
CCGAAGCTGG
AGTCCGAGTT
CGTCTACGGC
GACTACAAGG
TCTACGACGT
GCGCAAGATG
ATCGCCAAGA
GCGAGCAGGA
GATCGGCAAG
GCCACCGCGA
AGTACTTCTT
CTACTCCAAC
ATCATGAACT
TCTTCAAGAC
CGAGATCACC
CTGGCCAACG
GCGAGATCCG
CAAGCGGCCC
CTGATCGAGA
CCAACGGCGA
GACCGGCGAG
ATCGTCTGGG
ACAAGGGCCG
CGACTTCGCC
ACCGTCCGGA
AGGTGCTGTC
GATGCCGCAG
GTCAACATCG
TGAAGAAGAC
CGAGGTGCAG
ACCGGCGGCT
TCAGCAAGGA
GTCCATCCTC
CCCAAGCGCA
ACAGCGACAA
GCTGATCGCC
CGGAAGAAGG
ACTGGGACCC
GAAGAAGTAC
GGCGGCTTCG
ACAGCCCCAC
CGTCGCCTAC
TCCGTGCTGG
TCGTGGCGAA
GGTCGAGAAG
GGCAAGAGCA
AGAAGCTGAA
GTCCGTGAAG
GAGCTGCTCG
GCATCACCAT
CATGGAGCGC
TCCTCGTTCG
AGAAGAACCC
GATCGACTTC
CTGGAGGCCA
AGGGCTACAA
GGAGGTCAAG
AAGGACCTCA
TCATCAAGCT
GCCCAAGTAC
AGCCTGTTCG
AGCTGGAGAA
CGGCCGCAAG
CGGATGCTCG
CCTCCGCGGG
CGAGCTGCAA
AAGGGCAACG
AGCTGGCCCT
CCCGTCGAAG
TACGTCAACT
TCCTGTACCT
CGCGTCCCAC
TACGAGAAGC
TGAAGGGCTC
GCCCGAGGAC
AACGAGCAGA
AGCAGCTCTT
CGTGGAGCAG
CACAAGCACT
ACCTGGACGA
GATCATCGAG
CAGATCAGCG
AGTTCAGCAA
GCGCGTCATC
CTGGCCGACG
CGAACCTCGA
CAAGGTGCTG
TCCGCCTACA
ACAAGCACCG
CGACAAGCCG
ATCCGGGAGC
AGGCGGAGAA
CATCATCCAC
CTGTTCACCC
TCACCAACCT
GGGTGCCCCG
GCCGCCTTCA
AGTACTTCGA
CACCACCATC
GACCGCAAGC
GGTACACCTC
CACCAAGGAG
GTCCTCGACG
CGACCCTGAT
CCACCAGAGC
ATCACCGGCC
TGTACGAGAC
CCGCATCGAC
CTGAGCCAGC
TGGGCGGCGA
CTCGGGCGGC
TCCACCAACC
TGTCGGACAT
CATCGAGAAG
GAGACCGGCA
AGCAGCTGGT
GATCCAGGAG
TCCATCCTGA
TGCTGCCGGA
GGAAGTGGAG
GAAGTGATCG
GCAACAAGCC
GGAGTCGGAC
ATCCTGGTGC
ACACCGCCTA
CGACGAGTCG
ACCGACGAGA
ACGTCATGCT
GCTGACCTCG
GACGCCCCGG
AGTACAAGCC
GTGGGCCCTG
GTCATCCAGG
ACTCCAACGG
CGAGAACAAG
ATCAAGATGC
TGTGAGAATT
CAGATCTACG
CGTTCCCCGC
AAAAGCGGCC
TTTGACTCCC
TGCAAGCCTC
AGCGACCGAA
TATATCGGTT
ATGCGTGGGC
GATGGTTGTT
GTCATTGTCG
GCGCAACTAT
CGGTATCAAG
CTGTTTAAGA
AATTCACCTC
GAAAGCAAGC
TGATAAACCG
ATACAATTAA
AGGCTCCTTT
TGGAGCCTTT
TTTTAAGCTT
GCATGCCTGC
AGGTCGACGG
ATCTTTTCCG
CTGCATAACC
CTGCTTCGGG
GTCATTATAG
CGATTTTTTC
GGTATATCCA
TCCTTTTTCG
CACGATATAC
AGGATTTTGC
CAAAGGGTTC
GTGTAGACTT
TCCTTGGTGT
ATCCAACGGC
GTCAGCCGGG
CAGGATAGGT
GAAGTAGGCC
CACCCGCGAG
CGGGTGTTCC
TTCTTCACTG
TCCCTTATTC
GCACCTGGCG
GTGCTCAACG
GGAATCCTGC
TCTGCGAGGC
TGGCCGGCTA
CCGCCGGCGT
AACAGATGAG
GGCAAGCGGA
TGGCTGATGA
AACCAAGCCA
ACCAGGAAGG
GCAGCCCACC
TATCAAGGTG
TACTGCCTTC
CAGACGAACG
AAGAGCGATT
GAGGAAAAGG
CGGCGGCGGC
CGGCATGAGC
CTGTCGGCCT
ACCTGCTGGC
CGTCGGCCAG
GGCTACAAAA
TCACGGGCGT
CGTGGACTAT
GAGCACGTCC
GCGAGCTGGC
CCGCATCAAT
GGCGACCTGG
GCCGCCTGGG
CGGCCTGCTG
AAACTCTGGC
TCACCGACGA
CCCGCGCACG
GCGCGGTTCG
GTGATGCCAC
GATCCTCGCC
CTGCTGGCGA
AGATCGAAGA
GAAGCAGGAC
GAGCTTGGCA
AGGTCATGAT
GGGCGTGGTC
CGCCCGAGGG
CAGAGCCATG
ACTTTTTTAG
CCGCTAAAAC
GGCCGGGGGG
TGCGCGTGAT
TGCCAAGCAC
GTCCCCATGC
GCTCCATCAA
GAAGAGCGAC
TTCGCGGAGC
TGGTGAAGTA
CATCACCGAC
GAGCAAGGCA
AGACCGATCC
CCGGGGACCT
GCAGGTCGAC
TCTAGAGTTT
TAGAGCTAGA
AATAGCAAGT
TAAAATAAGG
CTAGTCCGTT
ATCAACTTGA
AAAAGTGGCA
CCGAGTCGGT
GCTAAAAAAA
AACCCCGCCC
CTGACAGGGC
GGGGTTTTTT
TTGTAGCCTC
AGGAGTTGTA
GCTGTTCACA
TTCGAACGGT
CTCTGCTTTG
ACAACATGCT
GTGCGGTGTT
GTAAAGTCGT
GGCCGCTAGC
TATTTACCCG
CAGGACATAT
CCACGCCCTC
CTACATCGAA
GCTGAAAGCA
CGAGATTCTT
CGCCCTCCGA
GAGCTGCATC
AGGTCGGAGA
CGCTGTCGAA
CTTTTCGATC
AGAAACTTCT
CGACAGACGT
CGCGGTGAGT
TCAGGCTTTT
TCATATCTCA
TTGCCCCCGG
ACGAGCGTCT
GCTCCGCCAT
TCGCCGTCCG
CCGTGCCAAT
CGGATCAGCC
GTCCAAATGC
GGGATTTTCG
TTAGTCGGAG
GCCAAACGGC
ATTGAGCGTC
AGCATATCAT
CAGCGAGCTG
AAGAAAGACA
ATCCCCGATC
CGCTCCACGT
GTTGCCCCAG
CAATCAGCGC
GACCTTGCCC
CTCCAACGTC
ATCTCGTTCT
CCGCTCATGA
GCTCAGCCAA
TCGACTGGCG
AGCGGCATCG
CATTCTTCGC
ATCCCGCCTC
TGGCGGATGC
AGGAAGATCA
ACGGATCTCG
GCCCAGTTGA
CCCAGGGCTG
TCGCCACAAT
GTCGCGGGAG
CGGATCAACC
GAGCAAAGGC
ATGACCGACT
GGACCTTCCT
TCTGAAGGCT
CTTCTCCTTG
AGCCACCTGT
CCGCCAAGGC
AAAGCGCTCA
CAGCAGTGGT
CATTCTCGAG
ATAATCGACG
CGTACCAACT
TGCCATCCTG
AAGAATGGTG
CAGTGTCTCG
GCACCCCATA
GGGAACCTTT
GCCATCAACT
CGGCAAGATG
CAGCGTCGTG
TTGGCATCGT
GTCCCACGCC
GAGGAGAAGT
ACCTGCCCAT
CGAGTTCATG
GACACGGGCG
ACCGGGCTTG
CAGGCGAGTG
AGGTGGCAGG
GGCAATGGAT
CAGAGATGAT
CTGCTCTGCC
TGTGGCCCCG
CTGCCGCAAA
GGCAAATGGA
TGGGCGCTGC
GCTTTACATT
TGGCAGGCGC
CAGAATGTGT
CAGAGACAAC
TCCAAGGTCC
GGTGTAACGG
GCGACGTGGC
AGGATCGAAC
GGCTCGTCGT
CCAGACCTGA
CCACGAGGGC
ATGACGAGCG
TCCCTCCCGG
ACCCAGCGCA
GCACGCAGGG
CCTCGATCAG
TCCAAGTGGC
CCATCTTCGA
GGGGCCGGAC
GCTACGGAAG
GAGCTGTGGA
CCAGCAGCAC
ACCGCCGGGG
GTAACCCCAA
GGTTGAGAAG
CTGACCGATG