名称: | pSABEd |
---|---|
别称: | Plasmid#133968 |
基因长: | |
质粒宿主: | 链霉菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AGCTCGGCTT
TTCGCCATTC
GTATTGCACG
ACATTGCACT
CCACCGCTGA
TGACATCAGT
CGATCATAGC
ACGATCAACG
GCACTGTTGC
AAATAGTCGG
TGGTGATAAA
CTTATCATCC
CCTTTTGCTG
ATGGAGCTGC
ACATGAACCC
ATTCAAAGGC
CGGCATTTTC
AGCGTGACAT
CATTCTGTGG
GCCGTACGCT
GGTACTGCAA
ATACGGCATC
AGTTACCGTG
AGCTGCAGGT
CGACGGATCC
CTTTAGTGAG
GGTTAATTGC
GGCCGCGAAT
TCTTGAAGAC
GAAAGGGCCT
CGTGATACGC
CTATTTTTAT
AGGTTAATGT
CATGATAATA
ATGGTTTCTT
AGACGTCAGG
TGGCACTTTT
CGGGGAAATG
TGCGCGGAAC
CCCTATTTGT
TTATTTTTCT
AAATACATTC
AAATATGTAT
CCGCTCATGA
GACAATAACC
CTGATAAATG
CTTCAATAAT
ATTGAAAAAG
GAAGAGTATG
AGTATTCAAC
ATTTCCGTGT
CGCCCTTATT
CCCTTTTTTG
CGGCATTTTG
CCTTCCTGTT
TTTGCTCACC
CAGAAACGCT
GGTGAAAGTA
AAAGATGCTG
AAGATCAGTT
GGGTGCACGA
GTGGGTTACA
TCGAACTGGA
TCTCAACAGC
GGTAAGATCC
TTGAGAGTTT
TCGCCCCGAA
GAACGTTTTC
CAATGATGAG
CACTTTTAAA
GTTCTGCTAT
GTGGCGCGGT
ATTATCCCGT
GTTGACGCCG
GGCAAGAGCA
ACTCGGTCGC
CGCATACACT
ATTCTCAGAA
TGACTTGGTT
GAGTACTCAC
CAGTCACAGA
AAAGCATCTT
ACGGATGGCA
TGACAGTAAG
AGAATTATGC
AGTGCTGCCA
TAACCATGAG
TGATAACACT
GCGGCCAACT
TACTTCTGAC
AACGATCGGA
GGACCGAAGG
AGCTAACCGC
TTTTTTGCAC
AACATGGGGG
ATCATGTAAC
TCGCCTTGAT
CGTTGGGAAC
CGGAGCTGAA
TGAAGCCATA
CCAAACGACG
AGCGTGACAC
CACGATGCCT
GCAGCAATGG
CAACAACGTT
GCGCAAACTA
TTAACTGGCG
AACTACTTAC
TCTAGCTTCC
CGGCAACAAT
TAATAGACTG
GATGGAGGCG
GATAAAGTTG
CAGGACCACT
TCTGCGCTCG
GCCCTTCCGG
CTGGCTGGTT
TATTGCTGAT
AAATCTGGAG
CCGGTGAGCG
TGGGTCTCGC
GGTATCATTG
CAGCACTGGG
GCCAGATGGT
AAGCCCTCCC
GTATCGTAGT
TATCTACACG
ACGGGGAGTC
AGGCAACTAT
GGATGAACGA
AATAGACAGA
TCGCTGAGAT
AGGTGCCTCA
CTGATTAAGC
ATTGGTAACT
GTCAGACCAA
GTTTACTCAT
ATATACTTTA
GATTGATTTA
AAACTTCATT
TTTAATTTAA
AAGGATCTAG
GTGAAGATCC
TTTTTGATAA
TCTCATGACC
AAAATCCCTT
AACGTGAGTT
TTCGTTCCAC
TGAGCGTCAG
ACCCCGTAGA
AAAGATCAAA
GGATCTTCTT
GAGATCCTTT
TTTTCTGCGC
GTAATCTGCT
GCTTGCAAAC
AAAAAAACCA
CCGCTACCAG
CGGTGGTTTG
TTTGCCGGAT
CAAGAGCTAC
CAACTCTTTT
TCCGAAGGTA
ACTGGCTTCA
GCAGAGCGCA
GATACCAAAT
ACTGTCCTTC
TAGTGTAGCC
GTAGTTAGGC
CACCACTTCA
AGAACTCTGT
AGCACCGCCT
ACATACCTCG
CTCTGCTAAT
CCTGTTACCA
GTGGCTGCTG
CCAGTGGCGA
TAAGTCGTGT
CTTACCGGGT
TGGACTCAAG
ACGATAGTTA
CCGGATAAGG
CGCAGCGGTC
GGGCTGAACG
GGGGGTTCGT
GCACACAGCC
CAGCTTGGAG
CGAACGACCT
ACACCGAACT
GAGATACCTA
CAGCGTGAGC
TATGAGAAAG
CGCCACGCTT
CCCGAAGGGA
GAAAGGCGGA
CAGGTATCCG
GTAAGCGGCA
GGGTCGGAAC
AGGAGAGCGC
ACGAGGGAGC
TTCCAGGGGG
AAACGCCTGG
TATCTTTATA
GTCCTGTCGG
GTTTCGCCAC
CTCTGACTTG
AGCGTCGATT
TTTGTGATGC
TCGTCAGGGG
GGCGGAGCCT
ATGGAAAAAC
GCCAGCAACG
CGGCCTTTTT
ACGGTTCCTG
GCCTTTTGCT
GGCCTTTTGC
TCACATGTTC
TTTCCTGCGT
TATCCCCTGA
TTCTGTGGAT
AACCGTATTA
CCGCCTTTGA
GTGAGCTGAT
ACCGCTCGCC
GCAGCCGAAC
GACCGAGCGC
AGCGAGTCAG
TGAGCGAGGA
AGCGGAAGAG
CGCTGACTTC
CGCGTTTCCA
GACTTTACGA
AACACGGAAA
CCGAAGACCA
TTCATGTTGT
TGCTCAGGTC
GCAGACGTTT
TGCAGCAGCA
GTCGCTTCAC
GTTCGCTCGC
GTATCGGTGA
TTCATTCTGC
TAACCAGTAA
GGCAACCCCG
CCAGCCTAGC
CGGGTCCTCA
ACGACAGGAG
CACGATCATG
CGCACCCGTC
AGATCCAGAC
ATGATAAGAT
ACATTGATGA
GTTTGGACAA
ACCACAACTA
GAATGCAGTG
AAAAAAATGC
TTTATTTGTG
AAATTTGTGA
TGCTATTGCT
TTATTTGTAA
CCATTATAAG
CTGCAATAAA
CAAGTTCCCG
CACGATCAGC
GTCCCGCCGA
CCAGGAGCAG
TGCGGCCAGC
ACTGCCGCTA
ACGCCTGGTC
CTGGTCCCGG
TCCTGGTGGT
GCATCAGTCC
TCCCCGTGAT
CACTTCGGCA
CCCACCGTAG
TGATCACCCC
CGACAGCGGA
TCAAGGGGTT
TGCGGGTCCC
GGTCGGCGCC
GGGCGGGGGA
GGCAGGAGCC
GCCGACGCTG
CCTCTGGGAC
GGGCCGGACG
GCAGGGGGAC
CGGCGGCCGG
GCGAGCTGCA
GCCGGGGGTC
CGGCAGGGCC
GGAGCGGGCG
GAACCGTGCT
CTGACCTGCG
GCCCGAGTTT
CGTCACGTGA
CGGAATGGAA
GGCTGCTGCA
TTTCGTCACG
TGACGTATCT
CGGCGAGCGA
CTGCCGACGC
CACGGCGGAC
ACGATCGCCT
CGCGCTGGCG
CCGGGCCTCG
TACGCCCGCT
GGCGGCAGGA
GCGGCGGCAG
TAGTCCCGGC
TCCGGCCGAC
GCCGGATTGC
TTGATCTCCG
AGCCGCACCA
GGCGCAGAGC
TTCGCGCCGT
CGGCGTCCCT
GGGGGTGGTG
GTGCTCATGG
CCGACGACCG
TACGCGGCAC
GTCTCGTAGC
GAGGCGAGTC
GGGCGCGAGG
TACCGCCTGC
ACGAAGTGCC
GGCGGGGCCG
ACCCCGGGCG
AGTAATCCCA
GGATTACTCC
CGCGGCTTCG
ACCCCGGCCG
CCGTCGCCGC
GTACGTCACC
GACCCCCGCC
GTACGTCACC
GGGATGACGT
ACGGCGGGGG
GGAGCGAGTT
AGTGCGAAGT
GGGCCCACTT
GCGAGCCGGG
CGATGTGCCG
GGCGGCCCGC
TCCTGGCGGT
CGTCGGCGTC
GTCGTCCTGG
TCGTCGTCCT
GCTCTCGCCG
TCGGCGTGCA
GTTGCTTCCT
CGCGGCGCTG
GGCGAGGGCG
GCGAGCATGT
CGGCGTACGC
CTCGGCCACC
TCCCCCGCCG
TGAGCACCAC
CACTGTGTCG
GCCGCGTCGG
CCAGCGCCAG
GACCTCCCGC
ACCCGTTCGC
CCACGGCCGC
CGAATCCTCG
TTGCCGTCCT
TGCCTTCGGC
GGCCCGGGTC
GCCTCGAGGT
CGAGGGCGCG
GCGGGTGACC
GCGTGCCATC
CGTCCTCGGT
CACGGCGACC
CCGGCCCGCA
GCTCCCCGCC
GTCGGCGTCG
GCCGCCAGGA
GCAGATCGAG
GTCGTCGGCC
TCGGTGTCGC
CGCCGTCGAG
CCCGAGCATC
TGCCGCAGGT
AGCGGGTCCA
TTCGATGGCC
CGGCGTCCCC
GGGTTGCCCG
CTCGTACTCG
TGCCAGCGCG
AGAGGTTCCA
CTCCAGCGAG
CCGACCCCGG
CGGCGTCGTC
CTCGGTCATG
CCGCCGGTCA
GGTCCCCGAT
CCGTCCGAGG
AGTTCGAACG
GGGCGACGTT
CCCGCCGGTC
GCCGTCTTGA
GGTCGGCGCG
GGCGAGTTCG
AGGGCGGGCG
CCTTCCCGTC
CTGGGTCTTG
GCGATGTACT
CGGCGAGGTC
GTTGGCGTCG
CGCTCGGTCT
CCAGCCGCTT
GAAGTCGACG
CCGTGCCGGT
CGTCGGGCGT
GAAGGCGGGG
TTGACCTTGC
GCAGGGCGGC
GGTCCACACG
GACCGCCAGT
GCCCCTGCCA
CTCGTCGAGC
GCGGCGCCGG
TCGGCTCGAA
GGTGGCGACG
ATCTGCTTCG
CGGACCGCTC
CCCCTCGGTC
CGGCCGCCGA
CCAGGACGAT
CGCGTGGATG
TGCGGGTGCC
AGCCGTTGAT
CTGCCCCACG
GTGACTTCGG
TCGCGCGGAT
CATGCCGACG
TACCCGATCC
GGTCTCGGAT
GCCCTCGCGG
TCGGCGGCCC
GGTGCCCGTC
CTTGGCCCGG
CGTCCGGCCC
ACGTGCCGCC
CGTGATCAGT
CGCTGGTAGG
CGCCCGGCCG
CCGGGGGCTG
TCCGGCGTCT
TCCGGGTGCC
CTGGAGGGCG
TCCATGAGGT
CCGCGAGCCG
GTCCGTGTGC
CCATGGCGGG
CCGTGAAGGT
GACCAGGTAG
GCGGTCCCCC
CGCGCTTGAT
CCACTCGACC
ACGGCGGCGG
TGATCTCCTC
GGCCCGCTTG
TGCCGGATCG
TGGCGGCGCA
GACCGGGCAG
AGCCAGATCC
GCCCGCACCG
CATCAGGCCC
AGGACCACGG
ACGTTCCGGC
CGCCGTCTGG
GCGACGATCA
CGCCGGAGGC
AGGGTCCATC
AGGGCGCGGC
CGCAGCCCTT
GCACGCGGCG
TCCCCGCTGA
TCCGCCACAG
CGTCCGGCGG
CGGCTGTACC
GGGCGGCTTT
CCGCAGTCGG
GCAGCGTCGC
TCCGCGACGT
GCTTCCTACT
TCCGAGAGGC
TGTCGCCTCT
CGGGCTCTCC
CCATCCACCC
CGTCCGGAGA
AACCGCAGGT
CGGAGGGGTG
CGGGAAACTC
TGTTGTTTCT
TTCCCAAGGT
GTTCGCTTTT
GCCTCGGGCG
GCATCTCGCG
TCACACGCGC
GATCGCCCGC
TTCGCTGCCA
TCCGGCAGCG
GTCTGAGCAG
TAGATACGCG
GCCGTTTGCC
CGGTGTGTGG
GCAATTGCGG
TCCCGCAGTG
GCAGCGGGGC
CCGGCGGGCC
GATCTGGCAA
TGCCTCGGCA
TCGCTCCGTA
CTCTGGGCAC
GAGCAACGTT
CCTGTCTCGC
CCGGCTAAGG
GGCGCGAGTC
TGGGAGCGGA
CGGGTCGGAG
GTGCGAAGTC
CGGCCCGTTG
CTCTTTGGTC
TGGTGGGAAT
CCTGGCACCA
ATCGGGCCAG
AGGTTCCCTC
CGCCACTCCC
GACGCCCCTT
GGGGCTGGTG
TGACTTGGAG
GGCCGAAGAG
AGCCCCGCCG
GTGATCCGGC
GGGGCTTTGA
CGTGCGGTCA
GTGCGTGTGT
CGGCGAGCGA
TGGCCACGAG
GCCCTGGAAG
CCGAGCGGTC
CGGCGAAGTC
GGCCCAGTCG
CAACCGGGCT
CAGCGCAGTG
GGCGGACCAG
CCACCGCCGT
TGGGGTCCTG
GACCAGGTTC
ACGGTCCCCT
CGGTCAGGCG
TCCGTCGAAG
TCGGTCATGG
TCGGTCTCCT
GGTGGGTGGG
GGCGGGGCGC
CAGCACGAAG
TGCCGGCGCC
CCGCGGGGGT
TGGTCGGGTC
AGGCGCCGAA
CCGGCGGGCG
GCGGCGGCGA
CCAGGCCGTC
GGCGGCGGCC
ATGGCGCGGT
CGCGGTCGGT
GGTGAGGGCG
GTGCGGTCGG
CGGCGGCCCA
GTCCTGCTCT
CACGCAACGT
CTACGTGGAC
GCTCAGGGCG
ACACGATCGA
GGTCGGGGAG
TCCGTCGCTC
CGGCGGGCCG
CTGGCGCGTC
CACCGGGACT
GATCAAGGCG
AATACTTCAT
ATGGCCCTGC
AGGCGGAAGT
CAGGTAGACA
CGACTTCCGC
TAGTCCTTGC
AAGGTCTGCT
GACGTGAGGC
GGGGCGGTCG
TTTTTGACCG
CCCTGCCTTC
GTCATGTAGG
CTCGCTCGCT
GTGCCTGGCG
TGTCATCAGA
CGCCCAGGTC
CCGGTGCCGT
GAGGCCCGGG
CCATCGAGCC
GGTGGTACGT
GGCTGCGGTC
CCCTTGTGAG
GGCTGCGCGC
CGTGTGCTGT
CCGGCGCGCA
CAGCCTTGAA
TCCACCCGCG
GGGGCCGGCC
GGTCTCCGTG
AGCTCGAGTA
GACGACGGAG
ACGTAATGTC
CGAGGTGGAG
TTCTCCCACG
AGTACTGGAT
GCGCCACGCC
CTGACCCTGG
CCAAGCGCGC
GTGGGACGAG
CGCGAGGTGC
CGGTGGGCGC
CGTGCTGGTC
CACAACAACC
GCGTGATCGG
CGAGGGCTGG
AACCGGCCGA
TCGGCCGGCA
CGACCCGACC
GCCCACGCCG
AGATCATGGC
CCTGCGCCAG
GGCGGCCTGG
TCATGCAGAA
CTACCGGCTG
ATCGACGCGA
CCCTGTACGT
GACCCTGGAG
CCGTGCGTCA
TGTGCGCGGG
CGCCATGATC
CACTCCCGCA
TCGGCCGGGT
GGTGTTCGGC
GCCCGGGACG
CCAAGACCGG
CGCCGCGGGC
TCCCTGATGG
ACGTGCTGCA
CCACCCGGGC
ATGAACCACC
GCGTCGAGAT
CACCGAGGGC
ATCCTGGCGG
ACGAGTGCGC
CGCGCTGCTG
TCGGACTTCT
TCCGGATGCG
CCGGCAGGAG
ATCAAGGCCC
AGAAGAAGGC
GCAGTCGTCC
ACCGACTCGG
GCGGCTCGTC
CGGCGGCTCG
TCCGGCTCCG
AGACCCCGGG
CACCTCGGAG
TCCGCGACCC
CGGAGTCGTC
CGGCGGCTCG
TCCGGCGGCT
CGTCCGAGGT
CGAATTTTCC
CATGAATACT
GGATGCGGCA
CGCGCTGACC
CTGGCCAAGC
GGGCCCGCGA
CGAGCGCGAG
GTGCCCGTCG
GCGCGGTGCT
GGTCCTGAAC
AACCGGGTCA
TTGGTGAGGG
CTGGAACCGC
GCCATCGGCC
TGCACGACCC
CACCGCGCAT
GCCGAAATCA
TGGCCCTCCG
GCAGGGCGGC
CTGGTGATGC
AGAACTACCG
CCTCATCGAC
GCCACCCTGT
ACGTGACCTT
CGAGCCCTGC
GTGATGTGTG
CGGGTGCCAT
GATCCACTCC
CGGATCGGCC
GCGTCGTGTT
CGGCGTGCGC
AACGCCAAGA
CCGGCGCGGC
CGGCTCCCTG
ATGGACGTCC
TGCACTACCC
CGGCATGAAC
CACCGCGTGG
AAATTACCGA
GGGCATCCTG
GCCGACGAGT
GCGCGGCCCT
GCTGTGCTAC
TTCTTCCGCA
TGCCGCGGCA
GGTGTTCAAC
GCGCAGAAGA
AGGCGCAGTC
GTCCACGGAT
TCGGGCGGCT
CGTCCGGCGG
CTCGTCCGGG
TCGGAAACCC
CGGGTACCTC
GGAGTCGGCC
ACCCCGGAGT
CGTCCGGCGG
CTCGTCCGGC
GGCTCGGACA
AGAAGTACAG
CATCGGCCTG
GCCATCGGCA
CCAACAGCGT
GGGCTGGGCG
GTCATCACCG
ACGAGTACAA
GGTCCCCTCC
AAGAAGTTCA
AGGTCCTGGG
CAACACCGAC
CGGCACTCGA
TCAAGAAGAA
CCTGATCGGC
GCCCTGCTCT
TCGACAGCGG
CGAGACCGCC
GAGGCGACCC
GCCTGAAGCG
GACCGCGCGC
CGGCGCTACA
CCCGGCGCAA
GAACCGCATC
TGCTACCTCC
AGGAAATCTT
CTCCAACGAG
ATGGCCAAGG
TCGACGACTC
GTTCTTCCAC
CGGCTCGAGG
AGAGCTTCCT
GGTGGAGGAG
GACAAGAAGC
ACGAGCGCCA
CCCGATCTTC
GGCAACATCG
TCGACGAGGT
GGCCTACCAC
GAGAAGTACC
CCACCATCTA
CCACCTCCGC
AAGAAGCTGG
TCGACTCGAC
CGACAAGGCG
GACCTGCGGC
TCATCTACCT
GGCCCTCGCG
CACATGATCA
AGTTCCGCGG
CCACTTCCTC
ATCGAGGGCG
ACCTGAACCC
GGACAACTCC
GACGTCGACA
AGCTCTTCAT
CCAGCTGGTG
CAGACCTACA
ACCAGCTGTT
CGAGGAGAAC
CCCATCAACG
CCAGCGGCGT
CGACGCCAAG
GCGATCCTCT
CCGCGCGCCT
GAGCAAGTCC
CGGCGCCTGG
AGAACCTCAT
CGCCCAGCTG
CCGGGCGAGA
AGAAGAACGG
CCTCTTCGGC
AACCTGATCG
CGCTGTCGCT
CGGCCTGACC
CCCAACTTCA
AGAGCAACTT
CGACCTGGCC
GAGGACGCGA
AGCTCCAGCT
GTCCAAGGAC
ACCTACGACG
ACGACCTGGA
CAACCTGCTC
GCCCAGATCG
GCGACCAGTA
CGCGGACCTC
TTCCTGGCCG
CGAAGAACCT
CTCGGACGCC
ATCCTGCTCA
GCGACATCCT
GCGGGTCAAC
ACCGAGATCA
CCAAGGCCCC
GCTGTCGGCG
AGCATGATCA
AGCGGTACGA
CGAGCACCAC
CAGGACCTGA
CCCTGCTCAA
GGCCCTCGTG
CGCCAGCAGC
TGCCCGAGAA
GTACAAGGAA
ATCTTCTTCG
ACCAGTCCAA
GAACGGCTAC
GCCGGCTACA
TCGACGGCGG
CGCGTCGCAG
GAGGAGTTCT
ACAAGTTCAT
CAAGCCGATC
CTGGAGAAGA
TGGACGGCAC
CGAGGAGCTG
CTCGTCAAGC
TGAACCGCGA
GGACCTGCTC
CGCAAGCAGC
GGACCTTCGA
CAACGGCTCC
ATCCCGCACC
AGATCCACCT
GGGCGAGCTG
CACGCCATCC
TCCGGCGCCA
GGAGGACTTC
TACCCCTTCC
TGAAGGACAA
CCGCGAGAAG
ATCGAGAAGA
TCCTGACCTT
CCGCATCCCG
TACTACGTCG
GCCCCCTGGC
CCGCGGCAAC
TCCCGGTTCG
CGTGGATGAC
CCGGAAGTCG
GAGGAGACCA
TCACCCCGTG
GAACTTCGAG
GAGGTCGTGG
ACAAGGGCGC
CTCCGCCCAG
TCGTTCATCG
AGCGCATGAC
CAACTTCGAC
AAGAACCTCC
CGAACGAGAA
GGTCCTGCCC
AAGCACTCCC
TGCTCTACGA
GTACTTCACC
GTGTACAACG
AGCTGACCAA
GGTCAAGTAC
GTGACCGAGG
GCATGCGGAA
GCCGGCCTTC
CTGTCGGGCG
AGCAGAAGAA
GGCGATCGTC
GACCTGCTCT
TCAAGACCAA
CCGCAAGGTC
ACCGTGAAGC
AGCTGAAGGA
GGACTACTTC
AAGAAGATCG
AGTGCTTCGA
CTCCGTCGAG
ATCAGCGGCG
TGGAGGACCG
CTTCAACGCC
TCCCTGGGCA
CCTACCACGA
CCTGCTCAAG
ATCATCAAGG
ACAAGGACTT
CCTCGACAAC
GAGGAGAACG
AGGACATCCT
GGAGGACATC
GTCCTCACCC
TGACCCTCTT
CGAGGACCGC
GAGATGATCG
AGGAGCGGCT
CAAGACCTAC
GCCCACCTGT
TCGACGACAA
GGTGATGAAG
CAGCTGAAGC
GGCGCCGGTA
CACCGGCTGG
GGCCGCCTCT
CCCGGAAGCT
GATCAACGGC
ATCCGGGACA
AGCAGAGCGG
CAAGACCATC
CTGGACTTCC
TCAAGTCCGA
CGGCTTCGCC
AACCGCAACT
TCATGCAGCT
CATCCACGAC
GACAGCCTGA
CCTTCAAGGA
GGACATCCAG
AAGGCCCAGG
TCTCGGGCCA
GGGCGACAGC
CTCCACGAGC
ACATCGCCAA
CCTGGCGGGC
TCCCCGGCGA
TCAAGAAGGG
CATCCTCCAG
ACCGTCAAGG
TCGTGGACGA
GCTGGTCAAG
GTGATGGGCC
GCCACAAGCC
CGAGAACATC
GTGATCGAGA
TGGCCCGGGA
GAACCAGACC
ACCCAGAAGG
GCCAGAAGAA
CTCGCGCGAG
CGGATGAAGC
GGATCGAGGA
GGGCATCAAG
GAGCTGGGCA
GCCAGATCCT
GAAGGAGCAC
CCGGTCGAGA
ACACCCAGCT
CCAGAACGAG
AAGCTGTACC
TCTACTACCT
CCAGAACGGC
CGCGACATGT
ACGTGGACCA
GGAGCTGGAC
ATCAACCGGC
TGTCCGACTA
CGACGTCGAC
GCCATCGTGC
CGCAGTCCTT
CCTGAAGGAC
GACTCGATCG
ACAACAAGGT
CCTGACCCGC
TCGGACAAGA
ACCGGGGCAA
GTCCGACAAC
GTGCCCTCGG
AGGAGGTCGT
GAAGAAGATG
AAGAACTACT
GGCGCCAGCT
GCTCAACGCC
AAGCTCATCA
CCCAGCGCAA
GTTCGACAAC
CTGACCAAGG
CCGAGCGGGG
CGGCCTGAGC
GAGCTGGACA
AGGCGGGCTT
CATCAAGCGC
CAGCTGGTCG
AGACCCGGCA
GATCACCAAG
CACGTGGCCC
AGATCCTGGA
CTCCCGGATG
AACACCAAGT
ACGACGAGAA
CGACAAGCTG
ATCCGCGAGG
TCAAGGTGAT
CACCCTCAAG
AGCAAGCTGG
TCTCCGACTT
CCGCAAGGAC
TTCCAGTTCT
ACAAGGTCCG
GGAGATCAAC
AACTACCACC
ACGCCCACGA
CGCGTACCTG
AACGCCGTCG
TGGGCACCGC
GCTGATCAAG
AAGTACCCGA
AGCTGGAGTC
CGAGTTCGTC
TACGGCGACT
ACAAGGTCTA
CGACGTGCGC
AAGATGATCG
CCAAGAGCGA
GCAGGAGATC
GGCAAGGCCA
CCGCGAAGTA
CTTCTTCTAC
TCCAACATCA
TGAACTTCTT
CAAGACCGAG
ATCACCCTGG
CCAACGGCGA
GATCCGCAAG
CGGCCCCTGA
TCGAGACCAA
CGGCGAGACC
GGCGAGATCG
TCTGGGACAA
GGGCCGCGAC
TTCGCCACCG
TCCGGAAGGT
GCTGTCGATG
CCGCAGGTCA
ACATCGTGAA
GAAGACCGAG
GTGCAGACCG
GCGGCTTCAG
CAAGGAGTCC
ATCCTCCCCA
AGCGCAACAG
CGACAAGCTG
ATCGCCCGGA
AGAAGGACTG
GGACCCGAAG
AAGTACGGCG
GCTTCGACAG
CCCCACCGTC
GCCTACTCCG
TGCTGGTCGT
GGCGAAGGTC
GAGAAGGGCA
AGAGCAAGAA
GCTGAAGTCC
GTGAAGGAGC
TGCTCGGCAT
CACCATCATG
GAGCGCTCCT
CGTTCGAGAA
GAACCCGATC
GACTTCCTGG
AGGCCAAGGG
CTACAAGGAG
GTCAAGAAGG
ACCTCATCAT
CAAGCTGCCC
AAGTACAGCC
TGTTCGAGCT
GGAGAACGGC
CGCAAGCGGA
TGCTCGCCTC
CGCGGGCGAG
CTGCAAAAGG
GCAACGAGCT
GGCCCTCCCG
TCGAAGTACG
TCAACTTCCT
GTACCTCGCG
TCCCACTACG
AGAAGCTGAA
GGGCTCGCCC
GAGGACAACG
AGCAGAAGCA
GCTCTTCGTG
GAGCAGCACA
AGCACTACCT
GGACGAGATC
ATCGAGCAGA
TCAGCGAGTT
CAGCAAGCGC
GTCATCCTGG
CCGACGCGAA
CCTCGACAAG
GTGCTGTCCG
CCTACAACAA
GCACCGCGAC
AAGCCGATCC
GGGAGCAGGC
GGAGAACATC
ATCCACCTGT
TCACCCTCAC
CAACCTGGGT
GCCCCGGCCG
CCTTCAAGTA
CTTCGACACC
ACCATCGACC
GCAAGCGGTA
CACCTCCACC
AAGGAGGTCC
TCGACGCGAC
CCTGATCCAC
CAGAGCATCA
CCGGCCTGTA
CGAGACCCGC
ATCGACCTGA
GCCAGCTGGG
CGGCGACTGA
GAATTCAGAT
CTACGCGTTC
CCCGCAAAAG
CGGCCTTTGA
CTCCCTGCAA
GCCTCAGCGA
CCGAATATAT
CGGTTATGCG
TGGGCGATGG
TTGTTGTCAT
TGTCGGCGCA
ACTATCGGTA
TCAAGCTGTT
TAAGAAATTC
ACCTCGAAAG
CAAGCTGATA
AACCGATACA
ATTAAAGGCT
CCTTTTGGAG
CCTTTTTTTA
AGCTTGCATG
CCTGCAGGTC
GACGGATCTT
TTCCGCTGCA
TAACCCTGCT
TCGGGGTCAT
TATAGCGATT
TTTTCGGTAT
ATCCATCCTT
TTTCGCACGA
TATACAGGAT
TTTGCCAAAG
GGTTCGTGTA
GACTTTCCTT
GGTGTATCCA
ACGGCGTCAG
CCGGGCAGGA
TAGGTGAAGT
AGGCCCACCC
GCGAGCGGGT
GTTCCTTCTT
CACTGTCCCT
TATTCGCACC
TGGCGGTGCT
CAACGGGAAT
CCTGCTCTGC
GAGGCTGGCC
GGCTACCGCC
GGCGTAACAG
ATGAGGGCAA
GCGGATGGCT
GATGAAACCA
AGCCAACCAG
GAAGGGCAGC
CCACCTATCA
AGGTGTACTG
CCTTCCAGAC
GAACGAAGAG
CGATTGAGGA
AAAGGCGGCG
GCGGCCGGCA
TGAGCCTGTC
GGCCTACCTG
CTGGCCGTCG
GCCAGGGCTA
CAAAATCACG
GGCGTCGTGG
ACTATGAGCA
CGTCCGCGAG
CTGGCCCGCA
TCAATGGCGA
CCTGGGCCGC
CTGGGCGGCC
TGCTGAAACT
CTGGCTCACC
GACGACCCGC
GCACGGCGCG
GTTCGGTGAT
GCCACGATCC
TCGCCCTGCT
GGCGAAGATC
GAAGAGAAGC
AGGACGAGCT
TGGCAAGGTC
ATGATGGGCG
TGGTCCGCCC
GAGGGCAGAG
CCATGACTTT
TTTAGCCGCT
AAAACGGCCG
GGGGGTGCGC
GTGATTGCCA
AGCACGTCCC
CATGCGCTCC
ATCAAGAAGA
GCGACTTCGC
GGAGCTGGTG
AAGTACATCA
CCGACGAGCA
AGGCAAGACC
GATCCCCGGG
GACCTGCAGG
TCGACTCTAG
AGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTATCAA
CTTGAAAAAG
TGGCACCGAG
TCGGTGCTAA
AAAAAAACCC
CGCCCCTGAC
AGGGCGGGGT
TTTTTTTGTA
GCCTCAGGAG
TTGTAGCTGT
TCACATTCGA
ACGGTCTCTG
CTTTGACAAC
ATGCTGTGCG
GTGTTGTAAA
GTCGTGGCCG
CTAGCTATTT
ACCCGCAGGA
CATATCCACG
CCCTCCTACA
TCGAAGCTGA
AAGCACGAGA
TTCTTCGCCC
TCCGAGAGCT
GCATCAGGTC
GGAGACGCTG
TCGAACTTTT
CGATCAGAAA
CTTCTCGACA
GACGTCGCGG
TGAGTTCAGG
CTTTTTCATA
TCTCATTGCC
CCCGGACGAG
CGTCTGCTCC
GCCATTCGCC
GTCCGCCGTG
CCAATCGGAT
CAGCCGTCCA
AATGCGGGAT
TTTCGTTAGT
CGGAGGCCAA
ACGGCATTGA
GCGTCAGCAT
ATCATCAGCG
AGCTGAAGAA
AGACAATCCC
CGATCCGCTC
CACGTGTTGC
CCCAGCAATC
AGCGCGACCT
TGCCCCTCCA
ACGTCATCTC
GTTCTCCGCT
CATGAGCTCA
GCCAATCGAC
TGGCGAGCGG
CATCGCATTC
TTCGCATCCC
GCCTCTGGCG
GATGCAGGAA
GATCAACGGA
TCTCGGCCCA
GTTGACCCAG
GGCTGTCGCC
ACAATGTCGC
GGGAGCGGAT
CAACCGAGCA
AAGGCATGAC
CGACTGGACC
TTCCTTCTGA
AGGCTCTTCT
CCTTGAGCCA
CCTGTCCGCC
AAGGCAAAGC
GCTCACAGCA
GTGGTCATTC
TCGAGATAAT
CGACGCGTAC
CAACTTGCCA
TCCTGAAGAA
TGGTGCAGTG
TCTCGGCACC
CCATAGGGAA
CCTTTGCCAT
CAACTCGGCA
AGATGCAGCG
TCGTGTTGGC
ATCGTGTCCC
ACGCCGAGGA
GAAGTACCTG
CCCATCGAGT
TCATGGACAC
GGGCGACCGG
GCTTGCAGGC
GAGTGAGGTG
GCAGGGGCAA
TGGATCAGAG
ATGATCTGCT
CTGCCTGTGG
CCCCGCTGCC
GCAAAGGCAA
ATGGATGGGC
GCTGCGCTTT
ACATTTGGCA
GGCGCCAGAA
TGTGTCAGAG
ACAACTCCAA
GGTCCGGTGT
AACGGGCGAC
GTGGCAGGAT
CGAACGGCTC
GTCGTCCAGA
CCTGACCACG
AGGGCATGAC
GAGCGTCCCT
CCCGGACCCA
GCGCAGCACG
CAGGGCCTCG
ATCAGTCCAA
GTGGCCCATC
TTCGAGGGGC
CGGACGCTAC
GGAAGGAGCT
GTGGACCAGC
AGCACACCGC
CGGGGGTAAC
CCCAAGGTTG
AGAAGCTGAC
CGATG