名称: | pET22b-SaCas9-ACRB(Steno)-sgRNA1-Lac-gyrA |
---|---|
别称: | |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | T7 |
复制子: | pBR322 |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | T7:TAATACGACTCACTATAGGG |
反向引物: | T7ter:TGCTAGTTATTGCTCAGCGG |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGCGAATGG
GACGCGCCCT
GTAGCGGCGC
ATTAAGCGCG
GCGGGTGTGG
TGGTTACGCG
CAGCGTGACC
GCTACACTTG
CCAGCGCCCT
AGCGCCCGCT
CCTTTCGCTT
TCTTCCCTTC
CTTTCTCGCC
ACGTTCGCCG
GCTTTCCCCG
TCAAGCTCTA
AATCGGGGGC
TCCCTTTAGG
GTTCCGATTT
AGTGCTTTAC
GGCACCTCGA
CCCCAAAAAA
CTTGATTAGG
GTGATGGTTC
ACGTAGTGGG
CCATCGCCCT
GATAGACGGT
TTTTCGCCCT
TTGACGTTGG
AGTCCACGTT
CTTTAATAGT
GGACTCTTGT
TCCAAACTGG
AACAACACTC
AACCCTATCT
CGGTCTATTC
TTTTGATTTA
TAAGGGATTT
TGCCGATTTC
GGCCTATTGG
TTAAAAAATG
AGCTGATTTA
ACAAAAATTT
AACGCGAATT
TTAACAAAAT
ATTAACGTTT
ACAATTTCAG
GTGGCACTTT
TCGGGGAAAT
GTGCGCGGAA
CCCCTATTTG
TTTATTTTTC
TAAATACATT
CAAATATGTA
TCCGCTCATG
AGACAATAAC
CCTGATAAAT
GCTTCAATAA
TATTGAAAAA
GGAAGAGTAT
GAGTATTCAA
CATTTCCGTG
TCGCCCTTAT
TCCCTTTTTT
GCGGCATTTT
GCCTTCCTGT
TTTTGCTCAC
CCAGAAACGC
TGGTGAAAGT
AAAAGATGCT
GAAGATCAGT
TGGGTGCACG
AGTGGGTTAC
ATCGAACTGG
ATCTCAACAG
CGGTAAGATC
CTTGAGAGTT
TTCGCCCCGA
AGAACGTTTT
CCAATGATGA
GCACTTTTAA
AGTTCTGCTA
TGTGGCGCGG
TATTATCCCG
TATTGACGCC
GGGCAAGAGC
AACTCGGTCG
CCGCATACAC
TATTCTCAGA
ATGACTTGGT
TGAGTACTCA
CCAGTCACAG
AAAAGCATCT
TACGGATGGC
ATGACAGTAA
GAGAATTATG
CAGTGCTGCC
ATAACCATGA
GTGATAACAC
TGCGGCCAAC
TTACTTCTGA
CAACGATCGG
AGGACCGAAG
GAGCTAACCG
CTTTTTTGCA
CAACATGGGG
GATCATGTAA
CTCGCCTTGA
TCGTTGGGAA
CCGGAGCTGA
ATGAAGCCAT
ACCAAACGAC
GAGCGTGACA
CCACGATGCC
TGCAGCAATG
GCAACAACGT
TGCGCAAACT
ATTAACTGGC
GAACTACTTA
CTCTAGCTTC
CCGGCAACAA
TTAATAGACT
GGATGGAGGC
GGATAAAGTT
GCAGGACCAC
TTCTGCGCTC
GGCCCTTCCG
GCTGGCTGGT
TTATTGCTGA
TAAATCTGGA
GCCGGTGAGC
GTGGGTCTCG
CGGTATCATT
GCAGCACTGG
GGCCAGATGG
TAAGCCCTCC
CGTATCGTAG
TTATCTACAC
GACGGGGAGT
CAGGCAACTA
TGGATGAACG
AAATAGACAG
ATCGCTGAGA
TAGGTGCCTC
ACTGATTAAG
CATTGGTAAC
TGTCAGACCA
AGTTTACTCA
TATATACTTT
AGATTGATTT
AAAACTTCAT
TTTTAATTTA
AAAGGATCTA
GGTGAAGATC
CTTTTTGATA
ATCTCATGAC
CAAAATCCCT
TAACGTGAGT
TTTCGTTCCA
CTGAGCGTCA
GACCCCGTAG
AAAAGATCAA
AGGATCTTCT
TGAGATCCTT
TTTTTCTGCG
CGTAATCTGC
TGCTTGCAAA
CAAAAAAACC
ACCGCTACCA
GCGGTGGTTT
GTTTGCCGGA
TCAAGAGCTA
CCAACTCTTT
TTCCGAAGGT
AACTGGCTTC
AGCAGAGCGC
AGATACCAAA
TACTGTCCTT
CTAGTGTAGC
CGTAGTTAGG
CCACCACTTC
AAGAACTCTG
TAGCACCGCC
TACATACCTC
GCTCTGCTAA
TCCTGTTACC
AGTGGCTGCT
GCCAGTGGCG
ATAAGTCGTG
TCTTACCGGG
TTGGACTCAA
GACGATAGTT
ACCGGATAAG
GCGCAGCGGT
CGGGCTGAAC
GGGGGGTTCG
TGCACACAGC
CCAGCTTGGA
GCGAACGACC
TACACCGAAC
TGAGATACCT
ACAGCGTGAG
CTATGAGAAA
GCGCCACGCT
TCCCGAAGGG
AGAAAGGCGG
ACAGGTATCC
GGTAAGCGGC
AGGGTCGGAA
CAGGAGAGCG
CACGAGGGAG
CTTCCAGGGG
GAAACGCCTG
GTATCTTTAT
AGTCCTGTCG
GGTTTCGCCA
CCTCTGACTT
GAGCGTCGAT
TTTTGTGATG
CTCGTCAGGG
GGGCGGAGCC
TATGGAAAAA
CGCCAGCAAC
GCGGCCTTTT
TACGGTTCCT
GGCCTTTTGC
TGGCCTTTTG
CTCACATGTT
CTTTCCTGCG
TTATCCCCTG
ATTCTGTGGA
TAACCGTATT
ACCGCCTTTG
AGTGAGCTGA
TACCGCTCGC
CGCAGCCGAA
CGACCGAGCG
CAGCGAGTCA
GTGAGCGAGG
AAGCGGAAGA
GCGCCTGATG
CGGTATTTTC
TCCTTACGCA
TCTGTGCGGT
ATTTCACACC
GCAATGGTGC
ACTCTCAGTA
CAATCTGCTC
TGATGCCGCA
TAGTTAAGCC
AGTATACACT
CCGCTATCGC
TACGTGACTG
GGTCATGGCT
GCGCCCCGAC
ACCCGCCAAC
ACCCGCTGAC
GCGCCCTGAC
GGGCTTGTCT
GCTCCCGGCA
TCCGCTTACA
GACAAGCTGT
GACCGTCTCC
GGGAGCTGCA
TGTGTCAGAG
GTTTTCACCG
TCATCACCGA
AACGCGCGAG
GCAGCTGCGG
TAAAGCTCAT
CAGCGTGGTC
GTGAAGCGAT
TCACAGATGT
CTGCCTGTTC
ATCCGCGTCC
AGCTCGTTGA
GTTTCTCCAG
AAGCGTTAAT
GTCTGGCTTC
TGATAAAGCG
GGCCATGTTA
AGGGCGGTTT
TTTCCTGTTT
GGTCACTGAT
GCCTCCGTGT
AAGGGGGATT
TCTGTTCATG
GGGGTAATGA
TACCGATGAA
ACGAGAGAGG
ATGCTCACGT
CACGGCCCCG
GGATGTGTTC
CATCAGCCCT
TCAATGCTGA
TGTCTTCATC
ATCAATATAC
GCCAGACAAC
CGTTGATGAC
TTCCGTCAGG
TTGTGCGGCG
GGATGTTGGT
TGCCATACCT
ACGGCGATAC
CGGAAGAACC
GTTCACCAGC
AGGTTAGGAA
TTTTGGTTGG
CATGACGTCC
GGAATTTTTT
CCGTGCCGTC
ATAGTTATCA
ACGAAATCGA
CCGTCTCTTT
TTCGAGATCG
GCCATCAGTT
CATGGGCAAT
TTTCGCCAGA
CGGATTTCCG
TATAACGCAT
TGCCGCCGCA
GAGTCGCCGT
CGATAGAACC
GAAGTTACCC
TGACCGTCTA
CCAGCATATA
ACGCAGCGAG
AATGGCTGCG
CCATGCGGAC
GATCGTGTCA
TAGACCGCCG
AGTCACCATG
GGGATGGTAT
TTACCGATTA
CGTCACCAAC
GACACGGGCA
GATTTTTTAT
AGGCTTTGTT
CCAGTCATTG
CCTAGTACGT
TCATGGCGTA
AAGTACGCGA
CGGTGTACCG
GCTTCAGGCC
ATCTCGGACA
TCTGGCAGCG
CACGGCCAAC
AATGACCGAC
ATCGCATAAT
CCAGATAGGA
GCTCTTCAGC
TCTTCCTCAA
TGTTGACCGG
TGTAATTTCT
CTCGCAAGGT
CGCTCATAGC
TGTTTCCTGT
GTGAAATTGT
TATCCGCTCA
CAATTCCACA
CAACATACGA
GCCGGAAGCA
TAAAGTGTAA
ACGGAAGGAG
CTGACTGGGT
TGAAGGCTCT
CAAGGGCATC
GGTCGAGATC
CCGGTGCCTA
ATGAGTGAGC
TAACTTACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCCAG
GGTGGTTTTT
CTTTTCACCA
GTGAGACGGG
CAACAGCTGA
TTGCCCTTCA
CCGCCTGGCC
CTGAGAGAGT
TGCAGCAAGC
GGTCCACGCT
GGTTTGCCCC
AGCAGGCGAA
AATCCTGTTT
GATGGTGGTT
AACGGCGGGA
TATAACATGA
GCTGTCTTCG
GTATCGTCGT
ATCCCACTAC
CGAGATATCC
GCACCAACGC
GCAGCCCGGA
CTCGGTAATG
GCGCGCATTG
CGCCCAGCGC
CATCTGATCG
TTGGCAACCA
GCATCGCAGT
GGGAACGATG
CCCTCATTCA
GCATTTGCAT
GGTTTGTTGA
AAACCGGACA
TGGCACTCCA
GTCGCCTTCC
CGTTCCGCTA
TCGGCTGAAT
TTGATTGCGA
GTGAGATATT
TATGCCAGCC
AGCCAGACGC
AGACGCGCCG
AGACAGAACT
TAATGGGCCC
GCTAACAGCG
CGATTTGCTG
GTGACCCAAT
GCGACCAGAT
GCTCCACGCC
CAGTCGCGTA
CCGTCTTCAT
GGGAGAAAAT
AATACTGTTG
ATGGGTGTCT
GGTCAGAGAC
ATCAAGAAAT
AACGCCGGAA
CATTAGTGCA
GGCAGCTTCC
ACAGCAATGG
CATCCTGGTC
ATCCAGCGGA
TAGTTAATGA
TCAGCCCACT
GACGCGTTGC
GCGAGAAGAT
TGTGCACCGC
CGCTTTACAG
GCTTCGACGC
CGCTTCGTTC
TACCATCGAC
ACCACCACGC
TGGCACCCAG
TTGATCGGCG
CGAGATTTAA
TCGCCGCGAC
AATTTGCGAC
GGCGCGTGCA
GGGCCAGACT
GGAGGTGGCA
ACGCCAATCA
GCAACGACTG
TTTGCCCGCC
AGTTGTTGTG
CCACGCGGTT
GGGAATGTAA
TTCAGCTCCG
CCATCGCCGC
TTCCACTTTT
TCCCGCGTTT
TCGCAGAAAC
GTGGCTGGCC
TGGTTCACCA
CGCGGGAAAC
GGTCTGATAA
GAGACACCGG
CATACTCTGC
GACATCGTAT
AACGTTACTG
GTTTCACATT
CACCACCCTG
AATTGACTCT
CTTCCGGGCG
CTATCATGCC
ATACCGCGAA
AGGTTTTGCG
CCATTCGATG
GTGTCCGGGA
TCTCGACGCT
CTCCCTTATG
CGACTCCTGC
ATTAGGAAGC
AGCCCAGTAG
TAGGTTGAGG
CCGTTGAGCA
CCGCCGCCGC
AAGGAATGGT
GCATGCCAGG
GCATCGCCCG
CGATCCGAAG
GGCGACACCA
CCGCGATGGT
GGTCGGCAAG
GACAACAAGG
TCGAAGTGCG
CCCGGTCAAG
GTCAGCCGCA
CGGTCGGCGA
CAAGTGGCTG
GTCGAGGACG
GTCTGAAGGC
CGGCGACAAG
GTCATCGTCG
AAGGCCTGCA
GAAGATCGGC
CCCGGCATGC
CGGTCAAGGC
CACCGAGAAG
GGCGCTGCTC
CGGCCAAGCC
GGCAGCGGCC
GCCCAGCCTG
CCGCCCCGGC
CGGCGACGCG
AAGTAAGCGG
AGAACCCCAT
GGCACGCTTT
TTCATCGATC
GACCCATCTT
CGCGTGGGTG
ATCGCCATCA
TCATCATGCT
CGCCGGCGGC
CTGGCCCTGT
TCAAGCTGCC
GGTCTCGATG
TACCCCAACG
TCGCACCACC
GGCGGTGGAA
ATCAGCGCCA
CCTACCCGGG
TGCATCGGCC
AAGGTGGTCG
AGGACTCGGT
GACGCAGATC
ATCGAGCAGA
ACATGAAGGG
CCTTGATGGT
CTGATCTACT
TCTCCTGACC
AGCAAGAACG
CGATCCTGAT
CGTGGAGTTC
GCCAAAGAGA
ACCTGGAGAA
GGGCGCAGGC
CTGATCGAAT
CGATCATGCA
TGCCGTGCGC
GACCGCCTGC
GTCCGATCGT
GATGACCTCG
CTGGCCTTCG
GCATGGGCGT
GGTACCGCTG
GCGATCTCCA
CCGGTGCCGG
TTCCGGCGCC
AAGCAGGCGA
TCGGCACCGG
CGTGCTCGGC
GGCATGATCG
TCGGCACCGT
GCTCGGTGTG
TTCTTCGTGC
CGCTGTTCTT
CGTGGTGGTG
CAGCGCGTGT
TCAAGCGCAA
ATCCACGACG
TGACCTGAAA
CGGTCATGCC
GGCCTCAGGC
TGGCGTTGTC
GAAACGGTAG
TGCCGGCCGC
TGGCCGGCAT
GACCTCCTGC
CATCCGGCAG
TACCGATGGA
AGTCATTCCC
ATGAAAAGTG
CATCCCTGTT
CCTTTCCATT
GCCGCTGTGC
TCGCGCTCGC
CGGCTGCTCC
ACCCTGGTGC
CGAAGAACAC
CGCTGTCGCC
CCGGCGATCC
CGGCGCCTGG
ATCCTTGACA
GCTAGCTCAG
TCCTAGGTAT
AATACTAGTG
ACCAGCACGA
TCGCTTCGAT
GTTTTAGAGC
TAGAAATAGC
AAGTTAAAAT
AAGGCTAGTC
CGTTATCAAC
TTGAAAAAGT
GGCACCGAGT
CGGTGCTTTT
TTTAGATCTC
GATCCCGCGA
AATTAATACG
ACTCACTATA
GGGGAATTGT
GAGCGGATAA
CAATTCCCCT
CTAGAAATAA
TTTTGTTTAA
CTTTAAGAAG
GAGATATACA
TATGAAATAC
CTGCTGCCGA
CCGCTGCTGC
TGGTCTGCTG
CTCCTCGCTG
CCCAGCCGGC
GATGGCCATG
GATATCGGAA
TTAATTCGGA
TCCGAATTCG
AGCTCCGTCG
ACAAGCTTGC
GGCCGCACTC
GAGCACCACC
ACCACCACCA
CATGGGCCGT
AAAAAACGTC
GCCAGCGCCG
CCGCGGTGGT
GGGAGCACGT
ATCCATATGA
TGTGCCGGAC
TACGCGGAGT
TCGGCAGTAT
GGCACCGAAG
AAAAAGCGTA
AAGTAGGTAT
TCACGGTGTC
CCCGCAGCAA
AACGTAATTA
CATTCTGGGA
CTGGATATCG
GTATTACGTC
AGTGGGGTAC
GGTATCATTG
ATTACGAAAC
GCGTGATGTA
ATTGACGCGG
GTGTCCGTCT
GTTCAAAGAA
GCGAATGTAG
AAAATAATGA
AGGACGCCGC
AGCAAACGTG
GAGCCCGCCG
TCTGAAGCGT
CGTCGTCGTC
ACCGCATCCA
ACGCGTCAAA
AAACTGCTGT
TTGATTACAA
CTTGCTGACC
GATCACAGTG
AACTGAGCGG
AATCAACCCG
TATGAAGCCC
GCGTGAAAGG
GCTGAGCCAG
AAACTGTCCG
AGGAAGAATT
CAGCGCCGCG
CTGCTCCACC
TGGCGAAACG
CCGCGGTGTG
CACAATGTGA
ACGAAGTCGA
AGAAGACACC
GGCAACGAAC
TGTCAACTAA
AGAACAGATC
AGTCGCAACA
GCAAAGCCCT
GGAAGAGAAA
TACGTGGCAG
AATTACAGCT
TGAACGCCTG
AAAAAAGACG
GGGAAGTGCG
TGGGAGCATT
AACCGTTTTA
AGACTAGTGA
TTACGTGAAG
GAAGCGAAAC
AGCTGTTGAA
AGTCCAGAAA
GCGTACCACC
AGCTGGATCA
ATCATTCATT
GATACATATA
TCGATTTGCT
GGAAACCCGC
CGTACCTATT
ATGAAGGCCC
TGGCGAAGGC
TCGCCTTTTG
GTTGGAAAGA
TATTAAAGAA
TGGTATGAAA
TGCTGATGGG
TCACTGTACT
TACTTTCCGG
AAGAACTGCG
CTCCGTCAAG
TATGCATATA
ATGCCGATCT
GTATAACGCG
CTGAATGATC
TGAATAACCT
GGTTATTACC
CGTGATGAAA
ATGAAAAGCT
GGAATACTAT
GAAAAATTTC
AAATTATCGA
AAATGTCTTT
AAACAGAAAA
AAAAACCGAC
ACTCAAACAA
ATCGCCAAAG
AAATTCTGGT
GAACGAGGAG
GATATTAAAG
GTTATCGCGT
TACGTCAACC
GGCAAACCGG
AATTTACGAA
CCTTAAAGTG
TATCATGATA
TTAAAGACAT
TACCGCGCGC
AAAGAGATTA
TCGAGAACGC
CGAGCTGCTG
GATCAGATTG
CGAAAATTCT
GACCATCTAT
CAATCCTCCG
AAGACATCCA
AGAAGAACTC
ACCAATTTAA
ATAGCGAGCT
GACTCAGGAG
GAGATCGAGC
AAATCTCCAA
CCTGAAGGGC
TACACCGGCA
CGCATAACCT
CAGCTTAAAA
GCTATTAATT
TGATTCTGGA
CGAACTTTGG
CACACTAACG
ATAACCAAAT
TGCTATTTTC
AATCGTCTGA
AACTGGTACC
TAAGAAAGTG
GATCTGTCGC
AGCAAAAAGA
AATTCCGACC
ACCCTGGTGG
ATGACTTTAT
CCTTAGTCCG
GTCGTAAAGC
GTAGCTTTAT
TCAAAGCATC
AAAGTCATCA
ACGCAATCAT
CAAAAAATAT
GGACTCCCAA
ATGATATCAT
TATCGAACTG
GCACGTGAGA
AAAACTCCAA
AGACGCTCAG
AAAATGATCA
ACGAAATGCA
GAAACGTAAT
CGTCAGACTA
ACGAACGTAT
CGAAGAGATT
ATTCGTACCA
CGGGCAAGGA
AAACGCAAAG
TATCTGATCG
AAAAAATTAA
GTTGCATGAC
ATGCAAGAAG
GCAAATGTCT
GTATAGCCTG
GAAGCGATTC
CCCTGGAGGA
CCTGCTGAAC
AACCCGTTTA
ACTATGAGGT
AGATCATATT
ATTCCACGCA
GTGTTAGCTT
TGATAACTCA
TTTAATAACA
AAGTCCTTGT
GAAACAGGAA
GAGAACAGTA
AGAAAGGTAA
CCGTACTCCA
TTCCAGTACT
TATCTAGTTC
TGATTCTAAA
ATCTCTTATG
AAACATTCAA
AAAACACATT
CTGAATCTGG
CTAAGGGGAA
AGGTCGCATT
AGCAAAACGA
AAAAAGAATA
TTTGCTGGAA
GAACGTGATA
TTAATCGTTT
TTCCGTGCAG
AAAGACTTCA
TCAATCGTAA
TCTGGTGGAT
ACCCGTTACG
CGACTCGCGG
GCTGATGAAC
CTGTTACGTT
CATACTTCCG
TGTTAACAAC
CTGGACGTCA
AGGTAAAGAG
CATCAATGGA
GGCTTTACCT
CTTTCCTGCG
CCGCAAATGG
AAATTTAAAA
AAGAACGCAA
CAAAGGCTAC
AAGCATCATG
CCGAGGATGC
CCTGATCATT
GCAAATGCCG
ACTTCATTTT
CAAAGAATGG
AAAAAACTGG
ATAAAGCAAA
AAAAGTGATG
GAAAATCAGA
TGTTTGAGGA
AAAGCAAGCA
GAATCTATGC
CGGAGATTGA
AACGGAACAG
GAATACAAAG
AAATCTTTAT
TACGCCTCAC
CAAATTAAAC
ACATCAAAGA
TTTTAAAGAC
TATAAATATT
CGCATCGTGT
GGATAAAAAA
CCAAACCGCG
AATTAATTAA
TGATACACTG
TACTCTACCC
GTAAAGATGA
TAAGGGAAAC
ACCCTGATTG
TTAATAACCT
GAACGGCCTG
TACGATAAAG
ATAACGACAA
GCTGAAAAAA
CTTATCAACA
AGTCGCCGGA
GAAACTGCTC
ATGTATCATC
ATGATCCACA
GACTTATCAG
AAACTGAAAC
TGATCATGGA
ACAGTATGGT
GATGAAAAAA
ATCCGCTGTA
TAAGTACTAT
GAAGAAACAG
GCAATTACCT
TACGAAATAC
TCTAAAAAAG
ACAATGGTCC
TGTGATTAAA
AAAATTAAAT
ACTACGGCAA
CAAACTGAAC
GCACATCTGG
ATATTACCGA
CGATTATCCA
AACTCGCGTA
ATAAAGTTGT
TAAACTCTCA
TTGAAGCCTT
ATCGTTTCGA
TGTATATCTC
GACAACGGTG
TTTACAAATT
CGTGACCGTG
AAGAATCTTG
ATGTTATCAA
AAAAGAAAAT
TACTACGAAG
TTAACTCTAA
ATGCTATGAG
GAGGCGAAAA
AATTGAAAAA
AATCTCTAAT
CAAGCCGAAT
TTATTGCAAG
CTTTTATAAC
AATGATCTGA
TCAAAATTAA
CGGGGAGCTG
TATCGCGTCA
TTGGTGTGAA
CAACGATTTG
CTCAATCGCA
TTGAAGTAAA
CATGATTGAC
ATCACATACC
GTGAATATCT
GGAGAACATG
AATGATAAAC
GTCCGCCTCG
CATTATCAAA
ACGATTGCAT
CTAAGACTCA
AAGCATTAAA
AAATATAGCA
CCGACATTTT
GGGTAACCTT
TATGAAGTTA
AATCTAAAAA
GCACCCCCAG
ATTATCAAAA
AAGGTAAGCG
TCCAGCAGCC
ACAAAAAAAG
CAGGCCAGGC
GAAGAAAAAA
AAACACCACC
ACCACCACCA
CTAGTGAGAT
CCGGCTGCTA
ACAAAGCCCG
AAAGGAAGCT
GAGTTGGCTG
CTGCCACCGC
TGAGCAATAA
CTAGCATAAC
CCCTTGGGGC
CTCTAAACGG
GTCTTGAGGG
GTTTTTTGCT
GAAAGGAGGA
ACTATATCCG
GAT