名称: | pDIRECT23C-CmYLCV-Csy4-FPA-sgRNA1-sgRNA2 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | Bar |
荧光标记: | |
启动子: | CmYLCV |
复制子: | pVS1 |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGGACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGC
ATTCTAGGTA
CTAAAACAAT
TCATCCAGTA
AAATATAATA
TTTTATTTTC
TCCCAATCAG
GCTTGATCCC
CAGTAAGTCA
AAAAATAGCT
CGACATACTG
TTCTTCCCCG
ATATCCTCCC
TGATCGACCG
GACGCAGAAG
GCAATGTCAT
ACCACTTGTC
CGCCCTGCCG
CTTCTCCCAA
GATCAATAAA
GCCACTTACT
TTGCCATCTT
TCACAAAGAT
GTTGCTGTCT
CCCAGGTCGC
CGTGGGAAAA
GACAAGTTCC
TCTTCGGGCT
TTTCCGTCTT
TAAAAAATCA
TACAGCTCGC
GCGGATCTTT
AAATGGAGTG
TCTTCTTCCC
AGTTTTCGCA
ATCCACATCG
GCCAGATCGT
TATTCAGTAA
GTAATCCAAT
TCGGCTAAGC
GGCTGTCTAA
GCTATTCGTA
TAGGGACAAT
CCGATATGTC
GATGGAGTGA
AAGAGCCTGA
TGCACTCCGC
ATACAGCTCG
ATAATCTTTT
CAGGGCTTTG
TTCATCTTCA
TACTCTTCCG
AGCAAAGGAC
GCCATCGGCC
TCACTCATGA
GCAGATTGCT
CCAGCCATCA
TGCCGTTCAA
AGTGCAGGAC
CTTTGGAACA
GGCAGCTTTC
CTTCCAGCCA
TAGCATCATG
TCCTTTTCCC
GTTCCACATC
ATAGGTGGTC
CCTTTATACC
GGCTGTCCGT
CATTTTTAAA
TATAGGTTTT
CATTTTCTCC
CACCAGCTTA
TATACCTTAG
CAGGAGACAT
TCCTTCCGTA
TCTTTTACGC
AGCGGTATTT
TTCGATCAGT
TTTTTCAATT
CCGGTGATAT
TCTCATTTTA
GCCATTTATT
ATTTCCTTCC
TCTTTTCTAC
AGTATTTAAA
GATACCCCAA
GAAGCTAATT
ATAACAAGAC
GAACTCCAAT
TCACTGTTCC
TTGCATTCTA
AAACCTTAAA
TACCAGAAAA
CAGCTTTTTC
AAAGTTGTTT
TCAAAGTTGG
CGTATAACAT
AGTATCGACG
GAGCCGATTT
TGAAACCGCG
GTGATCACAG
GCAGCAACGC
TCTGTCATCG
TTACAATCAA
CATGCTACCC
TCCGCGAGAT
CATCCGTGTT
TCAAACCCGG
CAGCTTAGTT
GCCGTTCTTC
CGAATAGCAT
CGGTAACATG
AGCAAAGTCT
GCCGCCTTAC
AACGGCTCTC
CCGCTGACGC
CGTCCCGGAC
TGATGGGCTG
CCTGTATCGA
GTGGTGATTT
TGTGCCGAGC
TGCCGGTCGG
GGAGCTGTTG
GCTGGCTGGT
GGCAGGATAT
ATTGTGGTGT
AAACAAATTG
ACGCTTAGAC
AACTTAATAA
CACATTGCGG
ACGTTTTTAA
TGTACTGAAT
TAACGCCGAA
TTAATTCGGG
GGATCTGGAT
TTTAGTACTG
GATTTTGGTT
TTAGGAATTA
GAAATTTTAT
TGATAGAAGT
ATTTTACAAA
TACAAATACA
TACTAAGGGT
TTCTTATATG
CTCAACACAT
GAGCGAAACC
CTATAGGAAC
CCTAATTCCC
TTATCTGGGA
ACTACTCACA
CATTATTATG
GAGAAACTCG
AGTCAAATCT
CGGTGACGGG
CAGGACCGGA
CGGGGCGGTA
CCGGCAGGCT
GAAGTCCAGC
TGCCAGAAAC
CCACGTCATG
CCAGTTCCCG
TGCTTGAAGC
CGGCCGCCCG
CAGCATGCCG
CGGGGGGCAT
ATCCGAGCGC
CTCGTGCATG
CGCACGCTCG
GGTCGTTGGG
CAGCCCGATG
ACAGCGACCA
CGCTCTTGAA
GCCCTGTGCC
TCCAGGGACT
TCAGCAGATG
GGTGTAGAGC
GTGGAGCCCA
GTCCCGTCCG
CTGGTGGCGG
GGGCTGACGT
ACACGGTCGA
CTCGGCCGTC
CAGTCGTAGG
CGTTGCGTGC
CTTCCAGGGG
CCCGCGTAGG
CGATGCCGGC
GACCTCGCCG
TCCACCTCGG
CGACGAGCCA
GGGATAGCGC
TCCCGCAGAC
GGACGAGGTC
GTCCGTCCAC
TCCTGCGGTT
CCTGCGGCTC
GGTACGGAAG
TTGACCGTGC
TTGTCTCGAT
GTAGTGGTTG
ACGATGGTGC
AGACCGCCGG
CATGTCCGCC
TCGGTGGCAC
GGCGGATGTC
GGCCGGGCGT
CGTTCTGGGC
TCATGGTAGA
CTCGAGAGAG
ATAGATTTGT
AGAGAGAGAC
TGGTGATTTC
AGCGTGTCCT
CTCCAAATGA
AATGAACTTC
CTTATATAGA
GGAAGGGTCT
TGCGAAGGAT
AGTGGGATTG
TGCGTCATCC
CTTACGTCAG
TGGAGATATC
ACATCAATCC
ACTTGCTTTG
AAGACGTGGT
TGGAACGTCT
TCTTTTTCCA
CGATGCTCCT
CGTGGGTGGG
GGTCCATCTT
TGGGACCACT
GTCGGCAGAG
GCATCTTGAA
CGATAGCCTT
TCCTTTATCG
CAATGATGGC
ATTTGTAGGT
GCCACCTTCC
TTTTCTACTG
TCCTTTTGAT
GAAGTGACAG
ATAGCTGGGC
AATGGAATCC
GAGGAGGTTT
CCCGATATTA
CCCTTTGTTG
AAAAGTCTCA
ATAGCCCTTT
GGTCTTCTGA
GACTGTATCT
TTGATATTCT
TGGAGTAGAC
GAGAGTGTCG
TGCTCCACCA
TGTTCACATC
AATCCACTTG
CTTTGAAGAC
GTGGTTGGAA
CGTCTTCTTT
TTCCACGATG
CTCCTCGTGG
GTGGGGGTCC
ATCTTTGGGA
CCACTGTCGG
CAGAGGCATC
TTGAACGATA
GCCTTTCCTT
TATCGCAATG
ATGGCATTTG
TAGGTGCCAC
CTTCCTTTTC
TACTGTCCTT
TTGATGAAGT
GACAGATAGC
TGGGCAATGG
AATCCGAGGA
GGTTTCCCGA
TATTACCCTT
TGTTGAAAAG
TCTCAATAGC
CCTTTGGTCT
TCTGAGACTG
TATCTTTGAT
ATTCTTGGAG
TAGACGAGAG
TGTCGTGCTC
CACCATGTTG
GCAAGCTGCT
CTAGCCAATA
CGCAAACCGC
CTCTCCCCGC
GCGTTGGCCG
ATTCATTAAT
GCAGCTGGCA
CGACAGGTTT
CCCGACTGGA
AAGCGGGCAG
TGAGCGCAAC
GCAATTAATG
TGAGTTAGCT
CACTCATTAG
GCACCCCAGG
CTTTACACTT
TATGCTTCCG
GCTCGTATGT
TGTGTGGAAT
TGTGAGCGGA
TAACAATTTC
ACACAGGAAA
CAGCTATGAC
ATGATTACGA
ATTCGAGCTC
GGTACCCGGG
GATCGGCGCG
CCAGATTTGC
CTTTTCAATT
TCAGAAAGAA
TGCTAACCCA
CAGATGGTTA
GAGAGGCTTA
CGCAGCAGGT
ATCATCAAGA
CGATCTACCC
GAGCAATAAT
CTCCAGGAAA
TCAAATACCT
TCCCAAGAAG
GTTAAAGATG
CAGTCAAAAG
ATTCAGGACT
AACTGCATCA
AGAACACAGA
GAAAGATATA
TTTCTCAAGA
TCAGAAGTAC
TATTCCAGTA
TGGACGATTC
AAGGCTTGCT
TCACAAACCA
AGGCAAGTAA
TAGAGATTGG
AGTCTCTAAA
AAGGTAGTTC
CCACTGAATC
AAAGGCCATG
GAGTCAAAGA
TTCAAATAGA
GGACCTAACA
GAACTCGCCG
TAAAGACTGG
CGAACAGTTC
ATACAGAGTC
TCTTACGACT
CAATGACAAG
AAGAAAATCT
TCGTCAACAT
GGTGGAGCAC
GACACACTTG
TCTACTCCAA
AAATATCAAA
GATACAGTCT
CAGAAGACCA
AAGGGCAATT
GAGACTTTTC
AACAAAGGGT
AATATCCGGA
AACCTCCTCG
GATTCCATTG
CCCAGCTATC
TGTCACTTTA
TTGTGAAGAT
AGTGGAAAAG
GAAGGTGGCT
CCTACAAATG
CCATCATTGC
GATAAAGGAA
AGGCCATCGT
TGAAGATGCC
TCTGCCGACA
GTGGTCCCAA
AGATGGACCC
CCACCCACGA
GGAGCATCGT
GGAAAAAGAA
GACGTTCCAA
CCACGTCTTC
AAAGCAAGTG
GATTGATGTG
ATATCTCCAC
TGACGTAAGG
GATGACGCAC
AATCCCACTA
TCCTTCGCAA
GACCCTTCCT
CTATATAAGG
AAGTTCATTT
CATTTGGAGA
GAACACGGGG
GACTCCTGCA
GGAAAAATGG
ATCATTATCT
TGATATTAGA
CTTAGACCTG
ATCCAGAATT
TCCACCAGCT
CAACTTATGT
CTGTTCTTTT
TGGAAAACTT
CATCAAGCTC
TTGTTGCTCA
AGGAGGAGAT
AGAATTGGAG
TTTCTTTTCC
TGATCTTGAT
GAATCAAGAT
CAAGACTTGG
AGAAAGACTT
AGAATTCATG
CTTCTGCTGA
TGATCTTAGA
GCTTTGCTTG
CTAGACCTTG
GCTTGAAGGA
CTTAGAGATC
ATCTTCAATT
TGGAGAACCA
GCTGTTGTTC
CACATCCAAC
TCCTTATAGA
CAAGTTTCAA
GAGTTCAAGC
TAAATCTAAT
CCAGAAAGAC
TTAGAAGAAG
ACTTATGAGA
AGACATGATC
TTTCTGAAGA
AGAAGCTAGA
AAAAGAATTC
CTGATACTGT
TGCTAGAGCT
TTGGATTTGC
CTTTTGTTAC
ACTTAGATCA
CAATCTACTG
GACAACATTT
TAGACTTTTT
ATTAGACATG
GACCACTTCA
AGTTACTGCT
GAAGAAGGAG
GATTTACTTG
TTATGGACTT
TCTAAGGGAG
GTTTTGTTCC
TTGGTTTGGA
TCTGGAGCTA
CTAATTTTTC
TCTTCTTAAG
CAAGCTGGAG
ATGTTGAAGA
AAATCCTGGA
CCCATGGATA
AGAAGTACTC
TATCGGACTC
GATATCGGAA
CTAACTCTGT
GGGATGGGCT
GTGATCACCG
ATGAGTACAA
GGTGCCATCT
AAGAAGTTCA
AGGTTCTCGG
AAACACCGAT
AGGCACTCTA
TCAAGAAAAA
CCTTATCGGT
GCTCTCCTCT
TCGATTCTGG
TGAAACTGCT
GAGGCTACCA
GACTCAAGAG
AACCGCTAGA
AGAAGGTACA
CCAGAAGAAA
GAACAGGATC
TGCTACCTCC
AAGAGATCTT
CTCTAACGAG
ATGGCTAAAG
TGGATGATTC
ATTCTTCCAC
AGGCTCGAAG
AGTCATTCCT
CGTGGAAGAA
GATAAGAAGC
ACGAGAGGCA
CCCTATCTTC
GGAAACATCG
TTGATGAGGT
GGCATACCAC
GAGAAGTACC
CTACTATCTA
CCACCTCAGA
AAGAAGCTCG
TTGATTCTAC
TGATAAGGCT
GATCTCAGGC
TCATCTACCT
CGCTCTCGCT
CACATGATCA
AGTTCAGAGG
ACACTTCCTC
ATCGAGGGTG
ATCTCAACCC
TGATAACTCT
GATGTGGATA
AGTTGTTCAT
CCAGCTCGTG
CAGACCTACA
ACCAGCTTTT
CGAAGAGAAC
CCTATCAACG
CTTCAGGTGT
GGATGCTAAG
GCTATCCTCT
CTGCTAGGCT
CTCTAAGTCA
AGAAGGCTTG
AGAACCTCAT
TGCTCAGCTC
CCTGGTGAGA
AGAAGAACGG
ACTTTTCGGA
AACTTGATCG
CTCTCTCTCT
CGGACTCACC
CCTAACTTCA
AGTCTAACTT
CGATCTCGCT
GAGGATGCAA
AGCTCCAGCT
CTCAAAGGAT
ACCTACGATG
ATGATCTCGA
TAACCTCCTC
GCTCAGATCG
GAGATCAGTA
CGCTGATTTG
TTCCTCGCTG
CTAAGAACCT
CTCTGATGCT
ATCCTCCTCA
GTGATATCCT
CAGAGTGAAC
ACCGAGATCA
CCAAGGCTCC
ACTCTCAGCT
TCTATGATCA
AGAGATACGA
TGAGCACCAC
CAGGATCTCA
CACTTCTCAA
GGCTCTTGTT
AGACAGCAGC
TCCCAGAGAA
GTACAAAGAG
ATTTTCTTCG
ATCAGTCTAA
GAACGGATAC
GCTGGTTACA
TCGATGGTGG
TGCATCTCAA
GAAGAGTTCT
ACAAGTTCAT
CAAGCCTATC
CTCGAGAAGA
TGGATGGAAC
CGAGGAACTC
CTCGTGAAGC
TCAATAGAGA
GGATCTTCTC
AGAAAGCAGA
GGACCTTCGA
TAACGGATCT
ATCCCTCATC
AGATCCACCT
CGGAGAGTTG
CACGCTATCC
TTAGAAGGCA
AGAGGATTTC
TACCCATTCC
TCAAGGATAA
CAGGGAAAAG
ATTGAGAAGA
TTCTCACCTT
CAGAATCCCT
TACTACGTGG
GACCTCTCGC
TAGAGGAAAC
TCAAGATTCG
CTTGGATGAC
CAGAAAGTCT
GAGGAAACCA
TCACCCCTTG
GAACTTCGAA
GAGGTGGTGG
ATAAGGGTGC
TAGTGCTCAG
TCTTTCATCG
AGAGGATGAC
CAACTTCGAT
AAGAACCTTC
CAAACGAGAA
GGTGCTCCCT
AAGCACTCTT
TGCTCTACGA
GTACTTCACC
GTGTACAACG
AGTTGACCAA
GGTTAAGTAC
GTGACCGAGG
GAATGAGGAA
GCCTGCTTTT
TTGTCAGGTG
AGCAAAAGAA
GGCTATCGTT
GATCTCTTGT
TCAAGACCAA
CAGAAAGGTG
ACCGTGAAGC
AGCTCAAAGA
GGATTACTTC
AAGAAAATCG
AGTGCTTCGA
TTCAGTTGAG
ATTTCTGGTG
TTGAGGATAG
GTTCAACGCA
TCTCTCGGAA
CCTACCACGA
TCTCCTCAAG
ATCATTAAGG
ATAAGGATTT
CTTGGATAAC
GAGGAAAACG
AGGATATCTT
GGAGGATATC
GTTCTTACCC
TCACCCTCTT
TGAAGATAGA
GAGATGATTG
AAGAAAGGCT
CAAGACCTAC
GCTCATCTCT
TCGATGATAA
GGTGATGAAG
CAGTTGAAGA
GAAGAAGATA
CACTGGTTGG
GGAAGGCTCT
CAAGAAAGCT
CATTAACGGA
ATCAGGGATA
AGCAGTCTGG
AAAGACAATC
CTTGATTTCC
TCAAGTCTGA
TGGATTCGCT
AACAGAAACT
TCATGCAGCT
CATCCACGAT
GATTCTCTCA
CCTTTAAAGA
GGATATCCAG
AAGGCTCAGG
TTTCAGGACA
GGGTGATAGT
CTCCATGAGC
ATATCGCTAA
CCTCGCTGGA
TCTCCTGCAA
TCAAGAAGGG
AATCCTCCAG
ACTGTGAAGG
TTGTGGATGA
GTTGGTGAAG
GTGATGGGAA
GGCATAAGCC
TGAGAACATC
GTGATCGAAA
TGGCTAGAGA
GAACCAGACC
ACTCAGAAGG
GACAGAAGAA
CTCTAGGGAA
AGGATGAAGA
GGATCGAGGA
AGGTATCAAA
GAGCTTGGAT
CTCAGATCCT
CAAAGAGCAC
CCTGTTGAGA
ACACTCAGCT
CCAGAATGAG
AAGCTCTACC
TCTACTACCT
CCAGAACGGA
AGGGATATGT
ATGTGGATCA
AGAGTTGGAT
ATCAACAGGC
TCTCTGATTA
CGATGTTGAT
CATATCGTGC
CACAGTCATT
CTTGAAGGAT
GATTCTATCG
ATAACAAGGT
GCTCACCAGG
TCTGATAAGA
ACAGGGGTAA
GAGTGATAAC
GTGCCAAGTG
AAGAGGTTGT
GAAGAAAATG
AAGAACTATT
GGAGGCAGCT
CCTCAACGCT
AAGCTCATCA
CTCAGAGAAA
GTTCGATAAC
TTGACTAAGG
CTGAGAGGGG
AGGACTCTCT
GAATTGGATA
AGGCAGGATT
CATCAAGAGG
CAGCTTGTGG
AAACCAGGCA
GATCACTAAG
CACGTTGCAC
AGATCCTCGA
TTCTAGGATG
AACACCAAGT
ACGATGAGAA
CGATAAGTTG
ATCAGGGAAG
TGAAGGTTAT
CACCCTCAAG
TCAAAGCTCG
TGTCTGATTT
CAGAAAGGAT
TTCCAATTCT
ACAAGGTGAG
GGAAATCAAC
AACTACCACC
ACGCTCACGA
TGCTTACCTT
AACGCTGTTG
TTGGAACCGC
TCTCATCAAG
AAGTATCCTA
AGCTCGAGTC
AGAGTTCGTG
TACGGTGATT
ACAAGGTGTA
CGATGTGAGG
AAGATGATCG
CTAAGTCTGA
GCAAGAGATC
GGAAAGGCTA
CCGCTAAGTA
TTTCTTCTAC
TCTAACATCA
TGAATTTCTT
CAAGACCGAG
ATTACCCTCG
CTAACGGTGA
GATCAGAAAG
AGGCCACTCA
TCGAGACAAA
CGGTGAAACA
GGTGAGATCG
TGTGGGATAA
GGGAAGGGAT
TTCGCTACCG
TTAGAAAGGT
GCTCTCTATG
CCACAGGTGA
ACATCGTTAA
GAAAACCGAG
GTGCAGACCG
GTGGATTCTC
TAAAGAGTCT
ATCCTCCCTA
AGAGGAACTC
TGATAAGCTC
ATTGCTAGGA
AGAAGGATTG
GGACCCTAAG
AAATACGGTG
GTTTCGATTC
TCCTACCGTG
GCTTACTCTG
TTCTCGTTGT
GGCTAAGGTT
GAGAAGGGAA
AGAGTAAGAA
GCTCAAGTCT
GTTAAGGAAC
TTCTCGGAAT
CACTATCATG
GAAAGGTCAT
CTTTCGAGAA
GAACCCAATC
GATTTCCTCG
AGGCTAAGGG
ATACAAAGAG
GTTAAGAAGG
ATCTCATCAT
CAAGCTCCCA
AAGTACTCAC
TCTTCGAACT
CGAGAACGGT
AGAAAGAGGA
TGCTCGCTTC
TGCTGGTGAG
CTTCAAAAGG
GAAACGAGCT
TGCTCTCCCA
TCTAAGTACG
TTAACTTTCT
TTACCTCGCT
TCTCACTACG
AGAAGTTGAA
GGGATCTCCA
GAAGATAACG
AGCAGAAGCA
ACTTTTCGTT
GAGCAGCACA
AGCACTACTT
GGATGAGATC
ATCGAGCAGA
TCTCTGAGTT
CTCTAAAAGG
GTGATCCTCG
CTGATGCAAA
CCTCGATAAG
GTGTTGTCTG
CTTACAACAA
GCACAGAGAT
AAGCCTATCA
GGGAACAGGC
AGAGAACATC
ATCCATCTCT
TCACCCTTAC
CAACCTCGGT
GCTCCTGCTG
CTTTCAAGTA
CTTCGATACA
ACCATCGATA
GGAAGAGATA
CACCTCTACC
AAAGAAGTGC
TCGATGCTAC
CCTCATCCAT
CAGTCTATCA
CTGGACTCTA
CGAGACTAGG
ATCGATCTCT
CACAGCTCGG
TGGTGATTCA
AGGGCTGATC
CTAAGAAGAA
GAGGAAGGTT
TGACTCGAGA
TATGAAGATG
AAGATGAAAT
ATTTGGTGTG
TCAAATAAAA
AGCTTGTGTG
CTTAAGTTTG
TGTTTTTTTC
TTGGCTTGTT
GTGTTATGAA
TTTGTGGCTT
TTTCTAATAT
TAAATGAATG
TAAGATCACA
TTATAATGAA
TAAACAAATG
TTTCTATAAT
CCATTGTGAA
TGTTTTGTTG
GATCTCTTCT
GCAGCATATA
ACTACTGTAT
GTGCTATGGT
ATGGACTATG
GAATATGATT
AAAGATAAGG
AGCTCCGGTG
ACGGACGGCG
CGCTGGCAGA
CATACTGTCC
CACAAATGAA
GATGGAATCT
GTAAAAGAAA
ACGCGTGAAA
TAATGCGTCT
GACAAAGGTT
AGGTCGGCTG
CCTTTAATCA
ATACCAAAGT
GGTCCCTACC
ACGATGGAAA
AACTGTGCAG
TCGGTTTGGC
TTTTTCTGAC
GAACAAATAA
GATTCGTGGC
CGACAGGTGG
GGGTCCACCA
TGTGAAGGCA
TCTTCAGACT
CCAATAATGG
AGCAATGACG
TAAGGGCTTA
CGAAATAAGT
AAGGGTAGTT
TGGGAAATGT
CCACTCACCC
GTCAGTCTAT
AAATACTTAG
CCCCTCCCTC
ATTGTTAAGG
GAGCAAAATC
TCAGAGAGAT
AGTCCTAGAG
AGAGAAAGAG
AGCAAGTAGC
CTAGAAGTAG
TCAAGGCGGC
GAAGTATTCA
GGCACGTGGC
CAGGAAGAAG
AAAAGCCAAG
ACGACGAAAA
CAGGTAAGAG
CTAAGCTTCC
TGCAGGTTCA
CTGCCGTATA
GGCAGTTGGG
TCATAACGAT
ATCTCGTTTT
AGAGCTAGAA
ATAGCAAGTT
AAAATAAGGC
TAGTCCGTTA
TCAACTTGAA
AAAGTGGCAC
CGAGTCGGTG
CGTTCACTGC
CGTATAGGCA
GTAACGCCAT
CGAAAGTACA
CGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTATCAA
CTTGAAAAAG
TGGCACCGAG
TCGGTGCGTT
CACTGCCGTA
TAGGCAGGTC
GATCGACAAG
CTCGAGTTTC
TCCATAATAA
TGTGTGAGTA
GTTCCCAGAT
AAGGGAATTA
GGGTTCCTAT
AGGGTTTCGC
TCATGTGTTG
AGCATATAAG
AAACCCTTAG
TATGTATTTG
TATTTGTAAA
ATACTTCTAT
CAATAAAATT
TCTAATTCCT
AAAACCAAAA
TCCAGTACTA
AAATCCAGAT
CCCCCGAATT
ACCGGTGGCA
CTGGCCGTCG
TTTTACAACG
TCGTGACTGG
GAAAACCCTG
GCGTTACCCA
ACTTAATCGC
CTTGCAGCAC
ATCCCCCTTT
CGCCAGCTGG
CGTAATAGCG
AAGAGGCCCG
CACCGATCGC
CCTTCCCAAC
AGTTGCGCAG
CCTGAATGGC
GAATGCTAGA
GCAGCTTGAG
CTTGGATCAG
ATTGTCGTTT
CCCGCCTTCA
GTTTAAACTA
TCAGTGTTTG
ACAGGATATA
TTGGCGGGTA
AACCTAAGAG
AAAAGAGCGT
TTATTAGAAT
AATCGGATAT
TTAAAAGGGC
GTGAAAAGGT
TTATCCGTTC
GTCCATTTGT
ATGTGCATGC
CAACCACAGG
GTTCCCCTCG
GGATCAAAGT
ACTTTGATCC
AACCCCTCCG
CTGCTATAGT
GCAGTCGGCT
TCTGACGTTC
AGTGCAGCCG
TCTTCTGAAA
ACGACATGTC
GCACAAGTCC
TAAGTTACGC
GACAGGCTGC
CGCCCTGCCC
TTTTCCTGGC
GTTTTCTTGT
CGCGTGTTTT
AGTCGCATAA
AGTAGAATAC
TTGCGACTAG
AACCGGAGAC
ATTACGCCAT
GAACAAGAGC
GCCGCCGCTG
GCCTGCTGGG
CTATGCCCGC
GTCAGCACCG
ACGACCAGGA
CTTGACCAAC
CAACGGGCCG
AACTGCACGC
GGCCGGCTGC
ACCAAGCTGT
TTTCCGAGAA
GATCACCGGC
ACCAGGCGCG
ACCGCCCGGA
GCTGGCCAGG
ATGCTTGACC
ACCTACGCCC
TGGCGACGTT
GTGACAGTGA
CCAGGCTAGA
CCGCCTGGCC
CGCAGCACCC
GCGACCTACT
GGACATTGCC
GAGCGCATCC
AGGAGGCCGG
CGCGGGCCTG
CGTAGCCTGG
CAGAGCCGTG
GGCCGACACC
ACCACGCCGG
CCGGCCGCAT
GGTGTTGACC
GTGTTCGCCG
GCATTGCCGA
GTTCGAGCGT
TCCCTAATCA
TCGACCGCAC
CCGGAGCGGG
CGCGAGGCCG
CCAAGGCCCG
AGGCGTGAAG
TTTGGCCCCC
GCCCTACCCT
CACCCCGGCA
CAGATCGCGC
ACGCCCGCGA
GCTGATCGAC
CAGGAAGGCC
GCACCGTGAA
AGAGGCGGCT
GCACTGCTTG
GCGTGCATCG
CTCGACCCTG
TACCGCGCAC
TTGAGCGCAG
CGAGGAAGTG
ACGCCCACCG
AGGCCAGGCG
GCGCGGTGCC
TTCCGTGAGG
ACGCATTGAC
CGAGGCCGAC
GCCCTGGCGG
CCGCCGAGAA
TGAACGCCAA
GAGGAACAAG
CATGAAACCG
CACCAGGACG
GCCAGGACGA
ACCGTTTTTC
ATTACCGAAG
AGATCGAGGC
GGAGATGATC
GCGGCCGGGT
ACGTGTTCGA
GCCGCCCGCG
CACGGCTCAA
CCGTGCGGCT
GCATGAAATC
CTGGCCGGTT
TGTCTGATGC
CAAGCTGGCG
GCCTGGCCGG
CCAGCTTGGC
CGCTGAAGAA
ACCGAGCGCC
GCCGTCTAAA
AAGGTGATGT
GTATTTGAGT
AAAACAGCTT
GCGTCATGCG
GTCGCTGCGT
ATATGATGCG
ATGAGTAAAT
AAACAAATAC
GCAAGGGGAA
CGCATGAAGG
TTATCGCTGT
ACTTAACCAG
AAAGGCGGGT
CAGGCAAGAC
GACCATCGCA
ACCCATCTAG
CCCGCGCCCT
GCAACTCGCC
GGGGCCGATG
TTCTGTTAGT
CGATTCCGAT
CCCCAGGGCA
GTGCCCGCGA
TTGGGCGGCC
GTGCGGGAAG
ATCAACCGCT
AACCGTTGTC
GGCATCGACC
GCCCGACGAT
TGACCGCGAC
GTGAAGGCCA
TCGGCCGGCG
CGACTTCGTA
GTGATCGACG
GAGCGCCCCA
GGCGGCGGAC
TTGGCTGTGT
CCGCGATCAA
GGCAGCCGAC
TTCGTGCTGA
TTCCGGTGCA
GCCAAGCCCT
TACGACATAT
GGGCCACCGC
CGACCTGGTG
GAGCTGGTTA
AGCAGCGCAT
TGAGGTCACG
GATGGAAGGC
TACAAGCGGC
CTTTGTCGTG
TCGCGGGCGA
TCAAAGGCAC
GCGCATCGGC
GGTGAGGTTG
CCGAGGCGCT
GGCCGGGTAC
GAGCTGCCCA
TTCTTGAGTC
CCGTATCACG
CAGCGCGTGA
GCTACCCAGG
CACTGCCGCC
GCCGGCACAA
CCGTTCTTGA
ATCAGAACCC
GAGGGCGACG
CTGCCCGCGA
GGTCCAGGCG
CTGGCCGCTG
AAATTAAATC
AAAACTCATT
TGAGTTAATG
AGGTAAAGAG
AAAATGAGCA
AAAGCACAAA
CACGCTAAGT
GCCGGCCGTC
CGAGCGCACG
CAGCAGCAAG
GCTGCAACGT
TGGCCAGCCT
GGCAGACACG
CCAGCCATGA
AGCGGGTCAA
CTTTCAGTTG
CCGGCGGAGG
ATCACACCAA
GCTGAAGATG
TACGCGGTAC
GCCAAGGCAA
GACCATTACC
GAGCTGCTAT
CTGAATACAT
CGCGCAGCTA
CCAGAGTAAA
TGAGCAAATG
AATAAATGAG
TAGATGAATT
TTAGCGGCTA
AAGGAGGCGG
CATGGAAAAT
CAAGAACAAC
CAGGCACCGA
CGCCGTGGAA
TGCCCCATGT
GTGGAGGAAC
GGGCGGTTGG
CCAGGCGTAA
GCGGCTGGGT
TGCCTGCCGG
CCCTGCAATG
GCACTGGAAC
CCCCAAGCCC
GAGGAATCGG
CGTGAGCGGT
CGCAAACCAT
CCGGCCCGGT
ACAAATCGGC
GCGGCGCTGG
GTGATGACCT
GGTGGAGAAG
TTGAAGGCCG
CGCAGGCCGC
CCAGCGGCAA
CGCATCGAGG
CAGAAGCACG
CCCCGGTGAA
TCGTGGCAAG
CGGCCGCTGA
TCGAATCCGC
AAAGAATCCC
GGCAACCGCC
GGCAGCCGGT
GCGCCGTCGA
TTAGGAAGCC
GCCCAAGGGC
GACGAGCAAC
CAGATTTTTT
CGTTCCGATG
CTCTATGACG
TGGGCACCCG
CGATAGTCGC
AGCATCATGG
ACGTGGCCGT
TTTCCGTCTG
TCGAAGCGTG
ACCGACGAGC
TGGCGAGGTG
ATCCGCTACG
AGCTTCCAGA
CGGGCACGTA
GAGGTTTCCG
CAGGGCCGGC
CGGCATGGCC
AGTGTGTGGG
ATTACGACCT
GGTACTGATG
GCGGTTTCCC
ATCTAACCGA
ATCCATGAAC
CGATACCGGG
AAGGGAAGGG
AGACAAGCCC
GGCCGCGTGT
TCCGTCCACA
CGTTGCGGAC
GTACTCAAGT
TCTGCCGGCG
AGCCGATGGC
GGAAAGCAGA
AAGACGACCT
GGTAGAAACC
TGCATTCGGT
TAAACACCAC
GCACGTTGCC
ATGCAGCGTA
CGAAGAAGGC
CAAGAACGGC
CGCCTGGTGA
CGGTATCCGA
GGGTGAAGCC
TTGATTAGCC
GCTACAAGAT
CGTAAAGAGC
GAAACCGGGC
GGCCGGAGTA
CATCGAGATC
GAGCTAGCTG
ATTGGATGTA
CCGCGAGATC
ACAGAAGGCA
AGAACCCGGA
CGTGCTGACG
GTTCACCCCG
ATTACTTTTT
GATCGATCCC
GGCATCGGCC
GTTTTCTCTA
CCGCCTGGCA
CGCCGCGCCG
CAGGCAAGGC
AGAAGCCAGA
TGGTTGTTCA
AGACGATCTA
CGAACGCAGT
GGCAGCGCCG
GAGAGTTCAA
GAAGTTCTGT
TTCACCGTGC
GCAAGCTGAT
CGGGTCAAAT
GACCTGCCGG
AGTACGATTT
GAAGGAGGAG
GCGGGGCAGG
CTGGCCCGAT
CCTAGTCATG
CGCTACCGCA
ACCTGATCGA
GGGCGAAGCA
TCCGCCGGTT
CCTAATGTAC
GGAGCAGATG
CTAGGGCAAA
TTGCCCTAGC
AGGGGAAAAA
GGTCGAAAAG
GCCTCTTTCC
TGTGGATAGC
ACGTACATTG
GGAACCCAAA
GCCGTACATT
GGGAACCGGA
ACCCGTACAT
TGGGAACCCA
AAGCCGTACA
TTGGGAACCG
GTCACACATG
TAAGTGACTG
ATATAAAAGA
GAAAAAAGGC
GATTTTTCCG
CCTAAAACTC
TTTAAAACTT
ATTAAAACTC
TTAAAACCCG
CCTGGCCTGT
GCATAACTGT
CTGGCCAGCG
CACAGCCCAA
GAGCTGCAAA
AAGCGCCTAC
CCTTCGGTCG
CTGCGCTCCC
TACGCCCCGC
CGCTTCGCGT
CGGCCTATCG
CGGCCGCTGG
CCGCTCAAAA
ATGGCTGGCC
TACGGCCAGG
CAATCTACCA
GGGCGCGGAC
AAGCCGCGCC
GTCGCCACTC
GACCGCCGGC
GCCCACATCA
AGGCACCCTG
CCTCGCGCGT
TTCGGTGATG
ACGGTGAAAA
CCTCTGACAC
ATGCAGCTCC
CGGAAACGGT
CACAGCTTGT
CTGTAAGCGG
ATGCCGGGAG
CAGACAAGCC
CGTCAGGGCG
CGTCAGCGGG
TGTTGGCGGG
TGTCGGGGCG
CAGCCATGAC
CCAGTCACGT
AGCGATAGCG
GAGTGTATAC
TGGCTTAACT
ATGCGGCATC
AGAGCAGATT
GTACTGAGAG
TGCACCATAT
GCGGTGTGAA
ATACCGCACA
GATGCGTAAG
GAGAAAATAC
CGCATCAGGC
CCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTAT