名称: | pXR001-U6-sgRNA-EF1a-CasRx-2A-EGFP |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGTTGAC
ATTGATTATT
GACTAGTTAT
TAATAGTAAT
CAATTACGGG
GTCATTAGTT
CATAGCCCAT
ATATGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTA
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGCGC
GTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
CTGATCTTCA
GACCTGGAGG
AGGAGATATG
AGGGACAATT
GGAGAAGTGA
ATTATATAAA
TATAAAGTAG
TAAAAATTGA
ACCATTAGGA
GTAGCACCCA
CCAAGGCAAA
GAGAAGAGTG
GTGCAGAGAG
AAAAAAGAGC
AGTGGGAATA
GGAGCTTTGT
TCCTTGGGTT
CTTGGGAGCA
GCAGGAAGCA
CTATGGGCGC
AGCGTCAATG
ACGCTGACGG
TACAGGCCAG
ACAATTATTG
TCTGGTATAG
TGCAGCAGCA
GAACAATTTG
CTGAGGGCTA
TTGAGGCGCA
ACAGCATCTG
TTGCAACTCA
CAGTCTGGGG
CATCAAGCAG
CTCCAGGCAA
GAATCCTGGC
TGTGGAAAGA
TACCTAAAGG
ATCAACAGCT
CCTGGGGATT
TGGGGTTGCT
CTGGAAAACT
CATTTGCACC
ACTGCTGTGC
CTTGGAATGC
TAGTTGGAGT
AATAAATCTC
TGGAACAGAT
TTGGAATCAC
ACGACCTGGA
TGGAGTGGGA
CAGAGAAATT
AACAATTACA
CAAGCTTAAT
ACACTCCTTA
ATTGAAGAAT
CGCAAAACCA
GCAAGAAAAG
AATGAACAAG
AATTATTGGA
ATTAGATAAA
TGGGCAAGTT
TGTGGAATTG
GTTTAACATA
ACAAATTGGC
TGTGGTATAT
AAAATTATTC
ATAATGATAG
TAGGAGGCTT
GGTAGGTTTA
AGAATAGTTT
TTGCTGTACT
TTCTATAGTG
AATAGAGTTA
GGCAGGGATA
TTCACCATTA
TCGTTTCAGA
CCCACCTCCC
AACCCCGAGG
GGACCCGACA
GGCCCGAAGG
AATAGAAGAA
GAAGGTGGAG
AGAGAGACAG
AGACAGATCC
ATTCGATTAG
TGAACGGATC
GGCACTGCGT
GCGCCAATTC
TGCAGACAAA
TGGCAGTATT
CATCCACAAT
TTTAAAAGAA
AAGGGGGGAT
TGGGGGGTAC
AGTGCAGGGG
AAAGAATAGT
AGACATAATA
GCAACAGACA
TACAAACTAA
AGAATTACAA
AAACAAATTA
CAAAAATTCA
AAATTTTCGG
GTTTATTACA
GGGACAGCAG
AGATCCAGGA
GGGCCTATTT
CCCATGATTC
CTTCATATTT
GCATATACGA
TACAAGGCTG
TTAGAGAGAT
AATTGGAATT
AATTTGACTG
TAAACACAAA
GATATTAGTA
CAAAATACGT
GACGTAGAAA
GTAATAATTT
CTTGGGTAGT
TTGCAGTTTT
AAAATTATGT
TTTAAAATGG
ACTATCATAT
GCTTACCGTA
ACTTGAAAGT
ATTTCGATTT
CTTGGCTTTA
TATATCTTGT
GGAAAGGACG
AAACACCGCA
AGTAAACCCC
TACCAACTGG
TCGGGGTTTG
AAACGGAGAC
GGACGTCTCT
CAAGTAAACC
CCTACCAACT
GGTCGGGGTT
TGAAACTTTT
TTTGGTTAAT
TAATGCAAAG
ATGGATAAAG
TTTTAAACAG
AGAGGAATCT
TTTCAGCTAA
TGGACCTTCT
AGGTCTTGAA
AGGAGTGGGA
ATTGGCTCCG
GTGCCCGTCA
GTGGGCAGAG
CGCACATCGC
CCACAGTCCC
CGAGAAGTTG
GGGGGAGGGG
TCGGCAATTG
AACCGGTGCC
TAGAGAAGGT
GGCGCGGGGT
AAACTGGGAA
AGTGATGTCG
TGTACTGGCT
CCGCCTTTTT
CCCGAGGGTG
GGGGAGAACC
GTATATAAGT
GCAGTAGTCG
CCGTGAACGT
TCTTTTTCGC
AACGGGTTTG
CCGCCAGAAC
ACAGGTAAGT
GCCGTGTGTG
GTTCCCGCGG
GCCTGGCCTC
TTTACGGGTT
ATGGCCCTTG
CGTGCCTTGA
ATTACTTCCA
CCTGGCTGCA
GTACGTGATT
CTTGATCCCG
AGCTTCGGGT
TGGAAGTGGG
TGGGAGAGTT
CGAGGCCTTG
CGCTTAAGGA
GCCCCTTCGC
CTCGTGCTTG
AGTTGAGGCC
TGGCCTGGGC
GCTGGGGCCG
CCGCGTGCGA
ATCTGGTGGC
ACCTTCGCGC
CTGTCTCGCT
GCTTTCGATA
AGTCTCTAGC
CATTTAAAAT
TTTTGATGAC
CTGCTGCGAC
GCTTTTTTTC
TGGCAAGATA
GTCTTGTAAA
TGCGGGCCAA
GATCTGCACA
CTGGTATTTC
GGTTTTTGGG
GCCGCGGGCG
GCGACGGGGC
CCGTGCGTCC
CAGCGCACAT
GTTCGGCGAG
GCGGGGCCTG
CGAGCGCGGC
CACCGAGAAT
CGGACGGGGG
TAGTCTCAAG
CTGGCCGGCC
TGCTCTGGTG
CCTGGCCTCG
CGCCGCCGTG
TATCGCCCCG
CCCTGGGCGG
CAAGGCTGGC
CCGGTCGGCA
CCAGTTGCGT
GAGCGGAAAG
ATGGCCGCTT
CCCGGCCCTG
CTGCAGGGAG
CTCAAAATGG
AGGACGCGGC
GCTCGGGAGA
GCGGGCGGGT
GAGTCACCCA
CACAAAGGAA
AAGGGCCTTT
CCGTCCTCAG
CCGTCGCTTC
ATGTGACTCC
ACGGAGTACC
GGGCGCCGTC
CAGGCACCTC
GATTAGTTCT
CGAGCTTTTG
GAGTACGTCG
TCTTTAGGTT
GGGGGGAGGG
GTTTTATGCG
ATGGAGTTTC
CCCACACTGA
GTGGGTGGAG
ACTGAAGTTA
GGCCAGCTTG
GCACTTGATG
TAATTCTCCT
TGGAATTTGC
CCTTTTTGAG
TTTGGATCTT
GGTTCATTCT
CAAGCCTCAG
ACAGTGGTTC
AAAGTTTTTT
TCTTCCATTT
CAGGTGTCGT
GACGTACGGC
CACCATGAGC
CCCAAGAAGA
AGAGAAAGGT
GGAGGCCAGC
ATCGAAAAAA
AAAAGTCCTT
CGCCAAGGGC
ATGGGCGTGA
AGTCCACACT
CGTGTCCGGC
TCCAAAGTGT
ACATGACAAC
CTTCGCCGAA
GGCAGCGACG
CCAGGCTGGA
AAAGATCGTG
GAGGGCGACA
GCATCAGGAG
CGTGAATGAG
GGCGAGGCCT
TCAGCGCTGA
AATGGCCGAT
AAAAACGCCG
GCTATAAGAT
CGGCAACGCC
AAATTCAGCC
ATCCTAAGGG
CTACGCCGTG
GTGGCTAACA
ACCCTCTGTA
TACAGGACCC
GTCCAGCAGG
ATATGCTCGG
CCTGAAGGAA
ACTCTGGAAA
AGAGGTACTT
CGGCGAGAGC
GCTGATGGCA
ATGACAATAT
TTGTATCCAG
GTGATCCATA
ACATCCTGGA
CATTGAAAAA
ATCCTCGCCG
AATACATTAC
CAACGCCGCC
TACGCCGTCA
ACAATATCTC
CGGCCTGGAT
AAGGACATTA
TTGGATTCGG
CAAGTTCTCC
ACAGTGTATA
CCTACGACGA
ATTCAAAGAC
CCCGAGCACC
ATAGGGCCGC
TTTCAACAAT
AACGATAAGC
TCATCAACGC
CATCAAGGCC
CAGTATGACG
AGTTCGACAA
CTTCCTCGAT
AACCCCAGAC
TCGGCTATTT
CGGCCAGGCC
TTTTTCAGCA
AGGAGGGCAG
AAATTACATC
ATCAATTACG
GCAACGAATG
CTATGACATT
CTGGCCCTCC
TGAGCGGACT
GAGGCACTGG
GTGGTCCATA
ACAACGAAGA
AGAGTCCAGG
ATCTCCAGGA
CCTGGCTCTA
CAACCTCGAT
AAGAACCTCG
ACAACGAATA
CATCTCCACC
CTCAACTACC
TCTACGACAG
GATCACCAAT
GAGCTGACCA
ACTCCTTCTC
CAAGAACTCC
GCCGCCAACG
TGAACTATAT
TGCCGAAACT
CTGGGAATCA
ACCCTGCCGA
ATTCGCCGAA
CAATATTTCA
GATTCAGCAT
TATGAAAGAG
CAGAAAAACC
TCGGATTCAA
TATCACCAAG
CTCAGGGAAG
TGATGCTGGA
CAGGAAGGAT
ATGTCCGAGA
TCAGGAAAAA
TCATAAGGTG
TTCGACTCCA
TCAGGACCAA
GGTCTACACC
ATGATGGACT
TTGTGATTTA
TAGGTATTAC
ATCGAAGAGG
ATGCCAAGGT
GGCTGCCGCC
AATAAGTCCC
TCCCCGATAA
TGAGAAGTCC
CTGAGCGAGA
AGGATATCTT
TGTGATTAAC
CTGAGGGGCT
CCTTCAACGA
CGACCAGAAG
GATGCCCTCT
ACTACGATGA
AGCTAATAGA
ATTTGGAGAA
AGCTCGAAAA
TATCATGCAC
AACATCAAGG
AATTTAGGGG
AAACAAGACA
AGAGAGTATA
AGAAGAAGGA
CGCCCCTAGA
CTGCCCAGAA
TCCTGCCCGC
TGGCCGTGAT
GTTTCCGCCT
TCAGCAAACT
CATGTATGCC
CTGACCATGT
TCCTGGATGG
CAAGGAGATC
AACGACCTCC
TGACCACCCT
GATTAATAAA
TTCGATAACA
TCCAGAGCTT
CCTGAAGGTG
ATGCCTCTCA
TCGGAGTCAA
CGCTAAGTTC
GTGGAGGAAT
ACGCCTTTTT
CAAAGACTCC
GCCAAGATCG
CCGATGAGCT
GAGGCTGATC
AAGTCCTTCG
CTAGAATGGG
AGAACCTATT
GCCGATGCCA
GGAGGGCCAT
GTATATCGAC
GCCATCCGTA
TTTTAGGAAC
CAACCTGTCC
TATGATGAGC
TCAAGGCCCT
CGCCGACACC
TTTTCCCTGG
ACGAGAACGG
AAACAAGCTC
AAGAAAGGCA
AGCACGGCAT
GAGAAATTTC
ATTATTAATA
ACGTGATCAG
CAATAAAAGG
TTCCACTACC
TGATCAGATA
CGGTGATCCT
GCCCACCTCC
ATGAGATCGC
CAAAAACGAG
GCCGTGGTGA
AGTTCGTGCT
CGGCAGGATC
GCTGACATCC
AGAAAAAACA
GGGCCAGAAC
GGCAAGAACC
AGATCGACAG
GTACTACGAA
ACTTGTATCG
GAAAGGATAA
GGGCAAGAGC
GTGAGCGAAA
AGGTGGACGC
TCTCACAAAG
ATCATCACCG
GAATGAACTA
CGACCAATTC
GACAAGAAAA
GGAGCGTCAT
TGAGGACACC
GGCAGGGAAA
ACGCCGAGAG
GGAGAAGTTT
AAAAAGATCA
TCAGCCTGTA
CCTCACCGTG
ATCTACCACA
TCCTCAAGAA
TATTGTCAAT
ATCAACGCCA
GGTACGTCAT
CGGATTCCAT
TGCGTCGAGC
GTGATGCTCA
ACTGTACAAG
GAGAAAGGCT
ACGACATCAA
TCTCAAGAAA
CTGGAAGAGA
AGGGATTCAG
CTCCGTCACC
AAGCTCTGCG
CTGGCATTGA
TGAAACTGCC
CCCGATAAGA
GAAAGGACGT
GGAAAAGGAG
ATGGCTGAAA
GAGCCAAGGA
GAGCATTGAC
AGCCTCGAGA
GCGCCAACCC
CAAGCTGTAT
GCCAATTACA
TCAAATACAG
CGACGAGAAG
AAAGCCGAGG
AGTTCACCAG
GCAGATTAAC
AGGGAGAAGG
CCAAAACCGC
CCTGAACGCC
TACCTGAGGA
ACACCAAGTG
GAATGTGATC
ATCAGGGAGG
ACCTCCTGAG
AATTGACAAC
AAGACATGTA
CCCTGTTCAG
AAACAAGGCC
GTCCACCTGG
AAGTGGCCAG
GTATGTCCAC
GCCTATATCA
ACGACATTGC
CGAGGTCAAT
TCCTACTTCC
AACTGTACCA
TTACATCATG
CAGAGAATTA
TCATGAATGA
GAGGTACGAG
AAAAGCAGCG
GAAAGGTGTC
CGAGTACTTC
GACGCTGTGA
ATGACGAGAA
GAAGTACAAC
GATAGGCTCC
TGAAACTGCT
GTGTGTGCCT
TTCGGCTACT
GTATCCCCAG
GTTTAAGAAC
CTGAGCATCG
AGGCCCTGTT
CGATAGGAAC
GAGGCCGCCA
AGTTCGACAA
GGAGAAAAAG
AAGGTGTCCG
GCAATTCCGG
ATCCGGACCT
AAGAAAAAGA
GGAAGGTGGC
GGCCGCTTAC
CCATACGATG
TTCCAGATTA
CGCTGCTAGC
GGCAGTGGAG
AGGGCAGAGG
AAGTCTGCTA
ACATGCGGTG
ACGTCGAGGA
GAATCCTGGC
CCAGTGAGCA
AGGGCGAGGA
GCTGTTCACC
GGGGTGGTGC
CCATCCTGGT
CGAGCTGGAC
GGCGACGTAA
ACGGCCACAA
GTTCAGCGTG
TCCGGCGAGG
GCGAGGGCGA
TGCCACCTAC
GGCAAGCTGA
CCCTGAAGTT
CATCTGCACC
ACCGGCAAGC
TGCCCGTGCC
CTGGCCCACC
CTCGTGACCA
CCCTGACCTA
CGGCGTGCAG
TGCTTCAGCC
GCTACCCCGA
CCACATGAAG
CAGCACGACT
TCTTCAAGTC
CGCCATGCCC
GAAGGCTACG
TCCAGGAGCG
CACCATCTTC
TTCAAGGACG
ACGGCAACTA
CAAGACCCGC
GCCGAGGTGA
AGTTCGAGGG
CGACACCCTG
GTGAACCGCA
TCGAGCTGAA
GGGCATCGAC
TTCAAGGAGG
ACGGCAACAT
CCTGGGGCAC
AAGCTGGAGT
ACAACTACAA
CAGCCACAAC
GTCTATATCA
TGGCCGACAA
GCAGAAGAAC
GGCATCAAGG
TGAACTTCAA
GATCCGCCAC
AACATCGAGG
ACGGCAGCGT
GCAGCTCGCC
GACCACTACC
AGCAGAACAC
CCCCATCGGC
GACGGCCCCG
TGCTGCTGCC
CGACAACCAC
TACCTGAGCA
CCCAGTCCGC
CCTGAGCAAA
GACCCCAACG
AGAAGCGCGA
TCACATGGTC
CTGCTGGAGT
TCGTGACCGC
CGCCGGGATC
ACTCTCGGCA
TGGACGAGCT
GTACAAGTAA
GAATTCGATA
TCAAGCTTAT
CGATAATCAA
CCTCTGGATT
ACAAAATTTG
TGAAAGATTG
ACTGGTATTC
TTAACTATGT
TGCTCCTTTT
ACGCTATGTG
GATACGCTGC
TTTAATGCCT
TTGTATCATG
CTATTGCTTC
CCGTATGGCT
TTCATTTTCT
CCTCCTTGTA
TAAATCCTGG
TTGCTGTCTC
TTTATGAGGA
GTTGTGGCCC
GTTGTCAGGC
AACGTGGCGT
GGTGTGCACT
GTGTTTGCTG
ACGCAACCCC
CACTGGTTGG
GGCATTGCCA
CCACCTGTCA
GCTCCTTTCC
GGGACTTTCG
CTTTCCCCCT
CCCTATTGCC
ACGGCGGAAC
TCATCGCCGC
CTGCCTTGCC
CGCTGCTGGA
CAGGGGCTCG
GCTGTTGGGC
ACTGACAATT
CCGTGGTGTT
GTCGGGGAAA
TCATCGTCCT
TTCCTTGGCT
GCTCGCCTGT
GTTGCCACCT
GGATTCTGCG
CGGGACGTCC
TTCTGCTACG
TCCCTTCGGC
CCTCAATCCA
GCGGACCTTC
CTTCCCGCGG
CCTGCTGCCG
GCTCTGCGGC
CTCTTCCGCG
TCTTCGCCTT
CGCCCTCAGA
CGAGTCGGAT
CTCCCTTTGG
GCCGCCTCCC
CGCATCGATA
CCGTCGACCT
CGAGACCTAG
AAAAACATGG
AGCAATCACA
AGTAGCAATA
CAGCAGCTAC
CAATGCTGAT
TGTGCCTGGC
TAGAAGCACA
AGAGGAGGAG
GAGGTGGGTT
TTCCAGTCAC
ACCTCAGGTA
CCTTTAAGAC
CAATGACTTA
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
GGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGACAAG
ATATCCTTGA
TCTGTGGATC
TACCACACAC
AAGGCTACTT
CCCTGATTGG
CAGAACTACA
CACCAGGGCC
AGGGATCAGA
TATCCACTGA
CCTTTGGATG
GTGCTACAAG
CTAGTACCAG
TTGAGCAAGA
GAAGGTAGAA
GAAGCCAATG
AAGGAGAGAA
CACCCGCTTG
TTACACCCTG
TGAGCCTGCA
TGGGATGGAT
GACCCGGAGA
GAGAAGTATT
AGAGTGGAGG
TTTGACAGCC
GCCTAGCATT
TCATCACATG
GCCCGAGAGC
TGCATCCGGA
CTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGG
GCCCGTTTAA
ACCCGCTGAT
CAGCCTCGAC
TGTGCCTTCT
AGTTGCCAGC
CATCTGTTGT
TTGCCCCTCC
CCCGTGCCTT
CCTTGACCCT
GGAAGGTGCC
ACTCCCACTG
TCCTTTCCTA
ATAAAATGAG
GAAATTGCAT
CGCATTGTCT
GAGTAGGTGT
CATTCTATTC
TGGGGGGTGG
GGTGGGGCAG
GACAGCAAGG
GGGAGGATTG
GGAAGACAAT
AGCAGGCATG
CTGGGGATGC
GGTGGGCTCT
ATGGCTTCTG
AGGCGGAAAG
AACCAGCTGG
GGCTCTAGGG
GGTATCCCCA
CGCGCCCTGT
AGCGGCGCAT
TAAGCGCGGC
GGGTGTGGTG
GTTACGCGCA
GCGTGACCGC
TACACTTGCC
AGCGCCCTAG
CGCCCGCTCC
TTTCGCTTTC
TTCCCTTCCT
TTCTCGCCAC
GTTCGCCGGC
TTTCCCCGTC
AAGCTCTAAA
TCGGGGGCTC
CCTTTAGGGT
TCCGATTTAG
TGCTTTACGG
CACCTCGACC
CCAAAAAACT
TGATTAGGGT
GATGGTTCAC
GTAGTGGGCC
ATCGCCCTGA
TAGACGGTTT
TTCGCCCTTT
GACGTTGGAG
TCCACGTTCT
TTAATAGTGG
ACTCTTGTTC
CAAACTGGAA
CAACACTCAA
CCCTATCTCG
GTCTATTCTT
TTGATTTATA
AGGGATTTTG
CCGATTTCGG
CCTATTGGTT
AAAAAATGAG
CTGATTTAAC
AAAAATTTAA
CGCGAATTAA
TTCTGTGGAA
TGTGTGTCAG
TTAGGGTGTG
GAAAGTCCCC
AGGCTCCCCA
GCAGGCAGAA
GTATGCAAAG
CATGCATCTC
AATTAGTCAG
CAACCAGGTG
TGGAAAGTCC
CCAGGCTCCC
CAGCAGGCAG
AAGTATGCAA
AGCATGCATC
TCAATTAGTC
AGCAACCATA
GTCCCGCCCC
TAACTCCGCC
CATCCCGCCC
CTAACTCCGC
CCAGTTCCGC
CCATTCTCCG
CCCCATGGCT
GACTAATTTT
TTTTATTTAT
GCAGAGGCCG
AGGCCGCCTC
TGCCTCTGAG
CTATTCCAGA
AGTAGTGAGG
AGGCTTTTTT
GGAGGCCTAG
GCTTTTGCAA
AAAGCTCCCG
GGAGCTTGTA
TATCCATTTT
CGGATCTGAT
CAGCACGTGT
TGACAATTAA
TCATCGGCAT
AGTATATCGG
CATAGTATAA
TACGACAAGG
TGAGGAACTA
AACCATGGCC
AAGTTGACCA
GTGCCGTTCC
GGTGCTCACC
GCGCGCGACG
TCGCCGGAGC
GGTCGAGTTC
TGGACCGACC
GGCTCGGGTT
CTCCCGGGAC
TTCGTGGAGG
ACGACTTCGC
CGGTGTGGTC
CGGGACGACG
TGACCCTGTT
CATCAGCGCG
GTCCAGGACC
AGGTGGTGCC
GGACAACACC
CTGGCCTGGG
TGTGGGTGCG
CGGCCTGGAC
GAGCTGTACG
CCGAGTGGTC
GGAGGTCGTG
TCCACGAACT
TCCGGGACGC
CTCCGGGCCG
GCCATGACCG
AGATCGGCGA
GCAGCCGTGG
GGGCGGGAGT
TCGCCCTGCG
CGACCCGGCC
GGCAACTGCG
TGCACTTCGT
GGCCGAGGAG
CAGGACTGAC
ACGTGCTACG
AGATTTCGAT
TCCACCGCCG
CCTTCTATGA
AAGGTTGGGC
TTCGGAATCG
TTTTCCGGGA
CGCCGGCTGG
ATGATCCTCC
AGCGCGGGGA
TCTCATGCTG
GAGTTCTTCG
CCCACCCCAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGT
ATACCGTCGA
CCTCTAGCTA
GAGCTTGGCG
TAATCATGGT
CATAGCTGTT
TCCTGTGTGA
AATTGTTATC
CGCTCACAAT
TCCACACAAC
ATACGAGCCG
GAAGCATAAA
GTGTAAAGCC
TGGGGTGCCT
AATGAGTGAG
CTAACTCACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GAACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GAC