名称: | T7-VEE-OKS-iG-ΔG |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达,干细胞重编程 |
片段类型: | ORF,IPS |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ATGGGCGGCG
CATGAGAGAA
GCCCAGACCA
ATTACCTACC
CAAAATGGAG
AAAGTTCACG
TTGACATCGA
GGAAGACAGC
CCATTCCTCA
GAGCTTTGCA
GCGGAGCTTC
CCGCAGTTTG
AGGTAGAAGC
CAAGCAGGTC
ACTGATAATG
ACCATGCTAA
TGCCAGAGCG
TTTTCGCATC
TGGCTTCAAA
ACTGATCGAA
ACGGAGGTGG
ACCCATCCGA
CACGATCCTT
GACATTGGAA
GTGCGCCCGC
CCGCAGAATG
TATTCTAAGC
ACAAGTATCA
TTGTATCTGT
CCGATGAGAT
GTGCGGAAGA
TCCGGACAGA
TTGTATAAGT
ATGCAACTAA
GCTGAAGAAA
AACTGTAAGG
AAATAACTGA
TAAGGAATTG
GACAAGAAAA
TGAAGGAGCT
GGCCGCCGTC
ATGAGCGACC
CTGACCTGGA
AACTGAGACT
ATGTGCCTCC
ACGACGACGA
GTCGTGTCGC
TACGAAGGGC
AAGTCGCTGT
TTACCAGGAT
GTATACGCGG
TTGACGGACC
GACAAGTCTC
TATCACCAAG
CCAATAAGGG
AGTTAGAGTC
GCCTACTGGA
TAGGCTTTGA
CACCACCCCT
TTTATGTTTA
AGAACTTGGC
TGGAGCATAT
CCATCATACT
CTACCAACTG
GGCCGACGAA
ACCGTGTTAA
CGGCTCGTAA
CATAGGCCTA
TGCAGCTCTG
ACGTTATGGA
GCGGTCACGT
AGAGGGATGT
CCATTCTTAG
AAAGAAGTAT
TTGAAACCAT
CCAACAATGT
TCTATTCTCT
GTTGGCTCGA
CCATCTACCA
CGAGAAGAGG
GACTTACTGA
GGAGCTGGCA
CCTGCCGTCT
GTATTTCACT
TACGTGGCAA
GCAAAATTAC
ACATGTCGGT
GTGAGACTAT
AGTTAGTTGC
GACGGGTACG
TCGTTAAAAG
AATAGCTATC
AGTCCAGGCC
TGTATGGGAA
GCCTTCAGGC
TATGCTGCTA
CGATGCACCG
CGAGGGATTC
TTGTGCTGCA
AAGTGACAGA
CACATTGAAC
GGGGAGAGGG
TCTCTTTTCC
CGTGTGCACG
TATGTGCCAG
CTACATTGTG
TGACCAAATG
ACTGGCATAC
TGGCAACAGA
TGTCAGTGCG
GACGACGCGC
AAAAACTGCT
GGTTGGGCTC
AACCAGCGTA
TAGTCGTCAA
CGGTCGCACC
CAGAGAAACA
CCAATACCAT
GAAAAATTAC
CTTTTGCCCG
TAGTGGCCCA
GGCATTTGCT
AGGTGGGCAA
AGGAATATAA
GGAAGATCAA
GAAGATGAAA
GGCCACTAGG
ACTACGAGAT
AGACAGTTAG
TCATGGGGTG
TTGTTGGGCT
TTTAGAAGGC
ACAAGATAAC
ATCTATTTAT
AAGCGCCCGG
ATACCCAAAC
CATCATCAAA
GTGAACAGCG
ATTTCCACTC
ATTCGTGCTG
CCCAGGATAG
GCAGTAACAC
ATTGGAGATC
GGGCTGAGAA
CAAGAATCAG
GAAAATGTTA
GAGGAGCACA
AGGAGCCGTC
ACCTCTCATT
ACCGCCGAGG
ACGTACAAGA
AGCTAAGTGC
GCAGCCGATG
AGGCTAAGGA
GGTGCGTGAA
GCCGAGGAGT
TGCGCGCAGC
TCTACCACCT
TTGGCAGCTG
ATGTTGAGGA
GCCCACTCTG
GAAGCCGATG
TCGACTTGAT
GTTACAAGAG
GCTGGGGCCG
GCTCAGTGGA
GACACCTCGT
GGCTTGATAA
AGGTTACCAG
CTACGATGGC
GAGGACAAGA
TCGGCTCTTA
CGCTGTGCTT
TCTCCGCAGG
CTGTACTCAA
GAGTGAAAAA
TTATCTTGCA
TCCACCCTCT
CGCTGAACAA
GTCATAGTGA
TAACACACTC
TGGCCGAAAA
GGGCGTTATG
CCGTGGAACC
ATACCATGGT
AAAGTAGTGG
TGCCAGAGGG
ACATGCAATA
CCCGTCCAGG
ACTTTCAAGC
TCTGAGTGAA
AGTGCCACCA
TTGTGTACAA
CGAACGTGAG
TTCGTAAACA
GGTACCTGCA
CCATATTGCC
ACACATGGAG
GAGCGCTGAA
CACTGATGAA
GAATATTACA
AAACTGTCAA
GCCCAGCGAG
CACGACGGCG
AATACCTGTA
CGACATCGAC
AGGAAACAGT
GCGTCAAGAA
AGAACTAGTC
ACTGGGCTAG
GGCTCACAGG
CGAGCTGGTG
GATCCTCCCT
TCCATGAATT
CGCCTACGAG
AGTCTGAGAA
CACGACCAGC
CGCTCCTTAC
CAAGTACCAA
CCATAGGGGT
GTATGGCGTG
CCAGGATCAG
GCAAGTCTGG
CATCATTAAA
AGCGCAGTCA
CCAAAAAAGA
TCTAGTGGTG
AGCGCCAAGA
AAGAAAACTG
TGCAGAAATT
ATAAGGGACG
TCAAGAAAAT
GAAAGGGCTG
GACGTCAATG
CCAGAACTGT
GGACTCAGTG
CTCTTGAATG
GATGCAAACA
CCCCGTAGAG
ACCCTGTATA
TTGACGAAGC
TTTTGCTTGT
CATGCAGGTA
CTCTCAGAGC
GCTCATAGCC
ATTATAAGAC
CTAAAAAGGC
AGTGCTCTGC
GGGGATCCCA
AACAGTGCGG
TTTTTTTAAC
ATGATGTGCC
TGAAAGTGCA
TTTTAACCAC
GAGATTTGCA
CACAAGTCTT
CCACAAAAGC
ATCTCTCGCC
GTTGCACTAA
ATCTGTGACT
TCGGTCGTCT
CAACCTTGTT
TTACGACAAA
AAAATGAGAA
CGACGAATCC
GAAAGAGACT
AAGATTGTGA
TTGACACTAC
CGGCAGTACC
AAACCTAAGC
AGGACGATCT
CATTCTCACT
TGTTTCAGAG
GGTGGGTGAA
GCAGTTGCAA
ATAGATTACA
AAGGCAACGA
AATAATGACG
GCAGCTGCCT
CTCAAGGGCT
GACCCGTAAA
GGTGTGTATG
CCGTTCGGTA
CAAGGTGAAT
GAAAATCCTC
TGTACGCACC
CACCTCAGAA
CATGTGAACG
TCCTACTGAC
CCGCACGGAG
GACCGCATCG
TGTGGAAAAC
ACTAGCCGGC
GACCCATGGA
TAAAAACACT
GACTGCCAAG
TACCCTGGGA
ATTTCACTGC
CACGATAGAG
GAGTGGCAAG
CAGAGCATGA
TGCCATCATG
AGGCACATCT
TGGAGAGACC
GGACCCTACC
GACGTCTTCC
AGAATAAGGC
AAACGTGTGT
TGGGCCAAGG
CTTTAGTGCC
GGTGCTGAAG
ACCGCTGGCA
TAGACATGAC
CACTGAACAA
TGGAACACTG
TGGATTATTT
TGAAACGGAC
AAAGCTCACT
CAGCAGAGAT
AGTATTGAAC
CAACTATGCG
TGAGGTTCTT
TGGACTCGAT
CTGGACTCCG
GTCTATTTTC
TGCACCCACT
GTTCCGTTAT
CCATTAGGAA
TAATCACTGG
GATAACTCCC
CGTCGCCTAA
CATGTACGGG
CTGAATAAAG
AAGTGGTCCG
TCAGCTCTCT
CGCAGGTACC
CACAACTGCC
TCGGGCAGTT
GCCACTGGAA
GAGTCTATGA
CATGAACACT
GGTACACTGC
GCAATTATGA
TCCGCGCATA
AACCTAGTAC
CTGTAAACAG
AAGACTGCCT
CATGCTTTAG
TCCTCCACCA
TAATGAACAC
CCACAGAGTG
ACTTTTCTTC
ATTCGTCAGC
AAATTGAAGG
GCAGAACTGT
CCTGGTGGTC
GGGGAAAAGT
TGTCCGTCCC
AGGCAAAATG
GTTGACTGGT
TGTCAGACCG
GCCTGAGGCT
ACCTTCAGAG
CTCGGCTGGA
TTTAGGCATC
CCAGGTGATG
TGCCCAAATA
TGACATAATA
TTTGTTAATG
TGAGGACCCC
ATATAAATAC
CATCACTATC
AGCAGTGTGA
AGACCATGCC
ATTAAGCTTA
GCATGTTGAC
CAAGAAAGCT
TGTCTGCATC
TGAATCCCGG
CGGAACCTGT
GTCAGCATAG
GTTATGGTTA
CGCTGACAGG
GCCAGCGAAA
GCATCATTGG
TGCTATAGCG
CGGCAGTTCA
AGTTTTCCCG
GGTATGCAAA
CCGAAATCCT
CACTTGAAGA
GACGGAAGTT
CTGTTTGTAT
TCATTGGGTA
CGATCGCAAG
GCCCGTACGC
ACAATTCTTA
CAAGCTTTCA
TCAACCTTGA
CCAACATTTA
TACAGGTTCC
AGACTCCACG
AAGCCGGATG
TGCACCCTCA
TATCATGTGG
TGCGAGGGGA
TATTGCCACG
GCCACCGAAG
GAGTGATTAT
AAATGCTGCT
AACAGCAAAG
GACAACCTGG
CGGAGGGGTG
TGCGGAGCGC
TGTATAAGAA
ATTCCCGGAA
AGCTTCGATT
TACAGCCGAT
CGAAGTAGGA
AAAGCGCGAC
TGGTCAAAGG
TGCAGCTAAA
CATATCATTC
ATGCCGTAGG
ACCAAACTTC
AACAAAGTTT
CGGAGGTTGA
AGGTGACAAA
CAGTTGGCAG
AGGCTTATGA
GTCCATCGCT
AAGATTGTCA
ACGATAACAA
TTACAAGTCA
GTAGCGATTC
CACTGTTGTC
CACCGGCATC
TTTTCCGGGA
ACAAAGATCG
ACTAACCCAA
TCATTGAACC
ATTTGCTGAC
AGCTTTAGAC
ACCACTGATG
CAGATGTAGC
CATATACTGC
AGGGACAAGA
AATGGGAAAT
GACTCTCAAG
GAAGCAGTGG
CTAGGAGAGA
AGCAGTGGAG
GAGATATGCA
TATCCGACGA
CTCTTCAGTG
ACAGAACCTG
ATGCAGAGCT
GGTGAGGGTG
CATCCGAAGA
GTTCTTTGGC
TGGAAGGAAG
GGCTACAGCA
CAAGCGATGG
CAAAACTTTC
TCATATTTGG
AAGGGACCAA
GTTTCACCAG
GCGGCCAAGG
ATATAGCAGA
AATTAATGCC
ATGTGGCCCG
TTGCAACGGA
GGCCAATGAG
CAGGTATGCA
TGTATATCCT
CGGAGAAAGC
ATGAGCAGTA
TTAGGTCGAA
ATGCCCCGTC
GAAGAGTCGG
AAGCCTCCAC
ACCACCTAGC
ACGCTGCCTT
GCTTGTGCAT
CCATGCCATG
ACTCCAGAAA
GAGTACAGCG
CCTAAAAGCC
TCACGTCCAG
AACAAATTAC
TGTGTGCTCA
TCCTTTCCAT
TGCCGAAGTA
TAGAATCACT
GGTGTGCAGA
AGATCCAATG
CTCCCAGCCT
ATATTGTTCT
CACCGAAAGT
GCCTGCGTAT
ATTCATCCAA
GGAAGTATCT
CGTGGAAACA
CCACCGGTAG
ACGAGACTCC
GGAGCCATCG
GCAGAGAACC
AATCCACAGA
GGGGACACCT
GAACAACCAC
CACTTATAAC
CGAGGATGAG
ACCAGGACTA
GAACGCCTGA
GCCGATCATC
ATCGAAGAGG
AAGAAGAGGA
TAGCATAAGT
TTGCTGTCAG
ATGGCCCGAC
CCACCAGGTG
CTGCAAGTCG
AGGCAGACAT
TCACGGGCCG
CCCTCTGTAT
CTAGCTCATC
CTGGTCCATT
CCTCATGCAT
CCGACTTTGA
TGTGGACAGT
TTATCCATAC
TTGACACCCT
GGAGGGAGCT
AGCGTGACCA
GCGGGGCAAC
GTCAGCCGAG
ACTAACTCTT
ACTTCGCAAA
GAGTATGGAG
TTTCTGGCGC
GACCGGTGCC
TGCGCCTCGA
ACAGTATTCA
GGAACCCTCC
ACATCCCGCT
CCGCGCACAA
GAACACCGTC
ACTTGCACCC
AGCAGGGCCT
GCTCGAGAAC
CAGCCTAGTT
TCCACCCCGC
CAGGCGTGAA
TAGGGTGATC
ACTAGAGAGG
AGCTCGAGGC
GCTTACCCCG
TCACGCACTC
CTAGCAGGTC
GGTCTCGAGA
ACCAGCCTGG
TCTCCAACCC
GCCAGGCGTA
AATAGGGTGA
TTACAAGAGA
GGAGTTTGAG
GCGTTCGTAG
CACAACAACA
ATGACGGTTT
GATGCGGGTG
CATACATCTT
TTCCTCCGAC
ACCGGTCAAG
GGCATTTACA
ACAAAAATCA
GTAAGGCAAA
CGGTGCTATC
CGAAGTGGTG
TTGGAGAGGA
CCGAATTGGA
GATTTCGTAT
GCCCCGCGCC
TCGACCAAGA
AAAAGAAGAA
TTACTACGCA
AGAAATTACA
GTTAAATCCC
ACACCTGCTA
ACAGAAGCAG
ATACCAGTCC
AGGAAGGTGG
AGAACATGAA
AGCCATAACA
GCTAGACGTA
TTCTGCAAGG
CCTAGGGCAT
TATTTGAAGG
CAGAAGGAAA
AGTGGAGTGC
TACCGAACCC
TGCATCCTGT
TCCTTTGTAT
TCATCTAGTG
TGAACCGTGC
CTTTTCAAGC
CCCAAGGTCG
CAGTGGAAGC
CTGTAACGCC
ATGTTGAAAG
AGAACTTTCC
GACTGTGGCT
TCTTACTGTA
TTATTCCAGA
GTACGATGCC
TATTTGGACA
TGGTTGACGG
AGCTTCATGC
TGCTTAGACA
CTGCCAGTTT
TTGCCCTGCA
AAGCTGCGCA
GCTTTCCAAA
GAAACACTCC
TATTTGGAAC
CCACAATACG
ATCGGCAGTG
CCTTCAGCGA
TCCAGAACAC
GCTCCAGAAC
GTCCTGGCAG
CTGCCACAAA
AAGAAATTGC
AATGTCACGC
AAATGAGAGA
ATTGCCCGTA
TTGGATTCGG
CGGCCTTTAA
TGTGGAATGC
TTCAAGAAAT
ATGCGTGTAA
TAATGAATAT
TGGGAAACGT
TTAAAGAAAA
CCCCATCAGG
CTTACTGAAG
AAAACGTGGT
AAATTACATT
ACCAAATTAA
AAGGACCAAA
AGCTGCTGCT
CTTTTTGCGA
AGACACATAA
TTTGAATATG
TTGCAGGACA
TACCAATGGA
CAGGTTTGTA
ATGGACTTAA
AGAGAGACGT
GAAAGTGACT
CCAGGAACAA
AACATACTGA
AGAACGGCCC
AAGGTACAGG
TGATCCAGGC
TGCCGATCCG
CTAGCAACAG
CGTATCTGTG
CGGAATCCAC
CGAGAGCTGG
TTAGGAGATT
AAATGCGGTC
CTGCTTCCGA
ACATTCATAC
ACTGTTTGAT
ATGTCGGCTG
AAGACTTTGA
CGCTATTATA
GCCGAGCACT
TCCAGCCTGG
GGATTGTGTT
CTGGAAACTG
ACATCGCGTC
GTTTGATAAA
AGTGAGGACG
ACGCCATGGC
TCTGACCGCG
TTAATGATTC
TGGAAGACTT
AGGTGTGGAC
GCAGAGCTGT
TGACGCTGAT
TGAGGCGGCT
TTCGGCGAAA
TTTCATCAAT
ACATTTGCCC
ACTAAAACTA
AATTTAAATT
CGGAGCCATG
ATGAAATCTG
GAATGTTCCT
CACACTGTTT
GTGAACACAG
TCATTAACAT
TGTAATCGCA
AGCAGAGTGT
TGAGAGAACG
GCTAACCGGA
TCACCATGTG
CAGCATTCAT
TGGAGATGAC
AATATCGTGA
AAGGAGTCAA
ATCGGACAAA
TTAATGGCAG
ACAGGTGCGC
CACCTGGTTG
AATATGGAAG
TCAAGATTAT
AGATGCTGTG
GTGGGCGAGA
AAGCGCCTTA
TTTCTGTGGA
GGGTTTATTT
TGTGTGACTC
CGTGACCGGC
ACAGCGTGCC
GTGTGGCAGA
CCCCCTAAAA
AGGCTGTTTA
AGCTTGGCAA
ACCTCTGGCA
GCAGACGATG
AACATGATGA
TGACAGGAGA
AGGGCATTGC
ATGAAGAGTC
AACACGCTGG
AACCGAGTGG
GTATTCTTTC
AGAGCTGTGC
AAGGCAGTAG
AATCAAGGTA
TGAAACCGTA
GGAACTTCCA
TCATAGTTAT
GGCCATGACT
ACTCTAGCTA
GCAGTGTTAA
ATCATTCAGC
TACCTGAGAG
GGGCCCCTAT
AACTCTCTAC
GGCTAACCTG
AATGGACTAC
GACATAGTCT
AGTCCGCCAA
GTCTAGCATA
TGGGCGCGTG
AATTCGCCAC
CATGGCGGGA
CACCTGGCTT
CGGATTTCGC
CTTCTCGCCC
CCTCCAGGTG
GTGGAGGTGA
TGGGCCAGGG
GGGCCGGAGC
CGGGCTGGGT
TGATCCTCGG
ACCTGGCTAA
GCTTCCAAGG
CCCTCCTGGA
GGGCCAGGAA
TCGGGCCGGG
GGTTGGGCCA
GGCTCTGAGG
TGTGGGGGAT
TCCCCCATGC
CCCCCGCCGT
ATGAGTTCTG
TGGGGGGATG
GCGTACTGTG
GGCCCCAGGT
TGGAGTGGGG
CTAGTGCCCC
AAGGCGGCTT
GGAGACCTCT
CAGCCTGAGG
GCGAAGCAGG
AGTCGGGGTG
GAGAGCAACT
CCGATGGGGC
CTCCCCGGAG
CCCTGCACCG
TCACCCCTGG
TGCCGTGAAG
CTGGAGAAGG
AGAAGCTGGA
GCAAAACCCG
GAGGAGTCCC
AGGACATCAA
AGCTCTGCAG
AAAGAACTCG
AGCAATTTGC
CAAGCTCCTG
AAGCAGAAGA
GGATCACCCT
GGGATATACA
CAGGCCGATG
TGGGGCTCAC
CCTGGGGGTT
CTATTTGGGA
AGGTATTCAG
CCAAACGACC
ATCTGCCGCT
TTGAGGCTCT
GCAGCTTAGC
TTCAAGAACA
TGTGTAAGCT
GCGGCCCTTG
CTGCAGAAGT
GGGTGGAGGA
AGCTGACAAC
AATGAAAATC
TTCAGGAGAT
ATGCAAAGCA
GAAACCCTCG
TGCAGGCCCG
AAAGAGAAAG
CGAACCAGTA
TCGAGAACCG
AGTGAGAGGC
AACCTGGAGA
ATTTGTTCCT
GCAGTGCCCG
AAACCCACAC
TGCAGCAGAT
CAGCCACATC
GCCCAGCAGC
TTGGGCTCGA
GAAGGATGTG
GTCCGAGTGT
GGTTCTGTAA
CCGGCGCCAG
AAGGGCAAGC
GATCAAGCAG
CGACTATGCA
CAACGAGAGG
ATTTTGAGGC
TGCTGGGTCT
CCTTTCTCAG
GGGGACCAGT
GTCCTTTCCT
CTGGCCCCAG
GGCCCCATTT
TGGTACCCCA
GGCTATGGGA
GCCCTCACTT
CACTGCACTG
TACTCCTCGG
TCCCTTTCCC
TGAGGGGGAA
GCCTTTCCCC
CTGTCTCCGT
CACCACTCTG
GGCTCTCCCA
TGCATTCAAA
CTCTAGTGAG
GGCAGAGGAA
GTCTGCTAAC
ATGCGGTGAC
GTCGAGGAGA
ATCCTGGCCC
ACAATTGATG
GCTGTCAGCG
ACGCGCTGCT
CCCATCTTTC
TCCACGTTCG
CGTCTGGCCC
GGCGGGAAGG
GAGAAGACAC
TGCGTCAAGC
AGGTGCCCCG
AATAACCGCT
GGCGGGAGGA
GCTCTCCCAC
ATGAAGCGAC
TTCCCCCAGT
GCTTCCCGGC
CGCCCCTATG
ACCTGGCGGC
GGCGACCGTG
GCCACAGACC
TGGAGAGCGG
CGGAGCCGGT
GCGGCTTGCG
GCGGTAGCAA
CCTGGCGCCC
CTACCTCGGA
GAGAGACCGA
GGAGTTCAAC
GATCTCCTGG
ACCTGGACTT
TATTCTCTCC
AATTCGCTGA
CCCATCCTCC
GGAGTCAGTG
GCCGCCACCG
TGTCCTCGTC
AGCGTCAGCC
TCCTCTTCGT
CGTCGCCGTC
GAGCAGCGGC
CCTGCCAGCG
CGCCCTCCAC
CTGCAGCTTC
ACCTATCCGA
TCCGGGCCGG
GAACGACCCG
GGCGTGGCGC
CGGGCGGCAC
GGGCGGAGGC
CTCCTCTATG
GCAGGGAGTC
CGCTCCCCCT
CCGACGGCTC
CCTTCAACCT
GGCGGACATC
AACGACGTGA
GCCCCTCGGG
CGGCTTCGTG
GCCGAGCTCC
TGCGGCCAGA
ATTGGACCCG
GTGTACATTC
CGCCGCAGCA
GCCGCAGCCG
CCAGGTGGCG
GGCTGATGGG
CAAGTTCGTG
CTGAAGGCGT
CGCTGAGCGC
CCCTGGCAGC
GAGTACGGCA
GCCCGTCGGT
CATCAGCGTC
AGCAAAGGCA
GCCCTGACGG
CAGCCACCCG
GTGGTGGTGG
CGCCCTACAA
CGGCGGGCCG
CCGCGCACGT
GCCCCAAGAT
CAAGCAGGAG
GCGGTCTCTT
CGTGCACCCA
CTTGGGCGCT
GGACCCCCTC
TCAGCAATGG
CCACCGGCCG
GCTGCACACG
ACTTCCCCCT
GGGGCGGCAG
CTCCCCAGCA
GGACTACCCC
GACCCTGGGT
CTTGAGGAAG
TGCTGAGCAG
CAGGGACTGT
CACCCTGCCC
TGCCGCTTCC
TCCCGGCTTC
CATCCCCACC
CGGGGCCCAA
TTACCCATCC
TTCCTGCCCG
ATCAGATGCA
GCCGCAAGTC
CCGCCGCTCC
ATTACCAAGA
GCTCATGCCA
CCCGGTTCCT
GCATGCCAGA
GGAGCCCAAG
CCAAAGAGGG
GAAGACGATC
GTGGCCCCGG
AAAAGGACCG
CCACCCACAC
TTGTGATTAC
GCGGGCTGCG
GCAAAACCTA
CACAAAGAGT
TCCCATCTCA
AGGCACACCT
GCGAACCCAC
ACAGGTGAGA
AACCTTACCA
CTGTGACTGG
GACGGCTGTG
GATGGAAATT
CGCCCGCTCA
GATGAACTGA
CCAGGCACTA
CCGTAAACAC
ACGGGGCACC
GCCCGTTCCA
GTGCCAAAAA
TGCGACCGAG
CATTTTCCAG
GTCGGACCAC
CTCGCCTTAC
ACATGAAGAG
GCATTTTTCT
AGACAATGTA
CTAACTACGC
TTTGTTGAAA
CTCGCTGGCG
ATGTTGAAAG
TAACCCCGGT
CCTGGCGCGC
CCATGTACAA
CATGATGGAG
ACGGAGCTGA
AGCCGCCGGG
CCCGCAGCAA
ACTTCGGGGG
GCGGCGGCGG
CAACTCCACC
GCGGCGGCGG
CCGGCGGCAA
CCAGAAAAAC
AGCCCGGACC
GCGTCAAGCG
GCCCATGAAT
GCCTTCATGG
TGTGGTCCCG
CGGGCAGCGG
CGCAAGATGG
CCCAGGAGAA
CCCCAAGATG
CACAACTCGG
AGATCAGCAA
GCGCCTGGGC
GCCGAGTGGA
AACTTTTGTC
GGAGACGGAG
AAGCGGCCGT
TCATCGACGA
GGCTAAGCGG
CTGCGAGCGC
TGCACATGAA
GGAGCACCCG
GATTATAAAT
ACCGGCCCCG
GCGGAAAACC
AAGACGCTCA
TGAAGAAGGA
TAAGTACACG
CTGCCCGGCG
GGCTGCTGGC
CCCCGGCGGC
AATAGCATGG
CGAGCGGGGT
CGGGGTGGGC
GCCGGCCTGG
GCGCGGGCGT
GAACCAGCGC
ATGGACAGTT
ACGCGCACAT
GAACGGCTGG
AGCAACGGCA
GCTACAGCAT
GATGCAGGAC
CAGCTGGGCT
ACCCGCAGCA
CCCGGGCCTC
AATGCGCACG
GCGCAGCGCA
GATGCAGCCC
ATGCACCGCT
ACGACGTGAG
CGCCCTGCAG
TACAACTCCA
TGACCAGCTC
GCAGACCTAC
ATGAACGGCT
CGCCCACCTA
CAGCATGTCC
TACTCGCAGC
AGGGCACCCC
TGGCATGGCT
CTTGGCTCCA
TGGGTTCGGT
GGTCAAGTCC
GAGGCCAGCT
CCAGCCCCCC
TGTGGTTACC
TCTTCCTCCC
ACTCCAGGGC
GCCCTGCCAG
GCCGGGGACC
TCCGGGACAT
GATCAGCATG
TATCTCCCCG
GCGCCGAGGT
GCCGGAACCC
GCCGCCCCCA
GCAGACTTCA
CATGTCCCAG
CACTACCAGA
GCGGCCCGGT
GCCCGGCACG
GCCATTAACG
GCACACTGCC
CCTCTCACAC
ATGTGAGCGG
CCATCGATAA
CTAACTTAAG
CTAGCAACGG
TTTCCCTCTA
GCGGGATCAA
TTCCGCCCCC
CCCCCCTAAC
GTTACTGGCC
GAAGCCGCTT
GGAATAAGGC
CGGTGTGCGT
TTGTCTATAT
GTTATTTTCC
ACCATATTGC
CGTCTTTTGG
CAATGTGAGG
GCCCGGAAAC
CTGGCCCTGT
CTTCTTGACG
AGCATTCCTA
GGGGTCTTTC
CCCTCTCGCC
AAAGGAATGC
AAGGTCTGTT
GAATGTCGTG
AAGGAAGCAG
TTCCTCTGGA
AGCTTCTTGA
AGACAAACAA
CGTCTGTAGC
GACCCTTTGC
AGGCAGCGGA
ACCCCCCACC
TGGCGACAGG
TGCCTCTGCG
GCCAAAAGCC
ACGTGTATAA
GATACACCTG
CAAAGGCGGC
ACAACCCCAG
TGCCACGTTG
TGAGTTGGAT
AGTTGTGGAA
AGAGTCAAAT
GGCTCTCCTC
AAGCGTATTC
AACAAGGGGC
TGAAGGATGC
CCAGAAGGTA
CCCCATTGTA
TGGGATCTGA
TCTGGGGCCT
CGGTGCACAT
GCTTTACATG
TGTTTAGTCG
AGGTTAAAAA
AACGTCTAGG
CCCCCCGAAC
CACGGGGACG
TGGTTTTCCT
TTGAAAAACA
CGATAATACC
AATTCGCCAC
CATGGCAGAG
GCCCGCACAT
CCCTGTCTGC
CCACTGTCGG
GGCCCGCTGG
CCACTGGCCT
GCACCCAGAC
CTGGACCTCC
CGGGCCGAAG
CCTCGCCACC
CCTGCGCCTT
CCTGCTACCT
TCTGGGCAGC
GAACCCAGCT
CTGGCCTGGG
CCTCCAGCCC
GAGACCCACC
TCCCCGAGGG
CAGCCTGAAG
CGGTGCTGCG
TCTTGGGCCT
ACCCCCCACC
TCCCCAGCCT
CCTCCTCACC
CTGTGCCTCC
TCCGACGTCA
CCTCCATCAT
CCGCTCCTCC
CAGACGTCTC
TGGTCACCTG
TGTAAATGGA
CTCCGGAGCC
CCCCTCTGAC
GGGAGATCTG
GGGGGCCCTT
CCAAGCGGGC
CCGGCCTGGC
CCTGCATCGA
CGGACAGCCA
TGAGGGCAGC
TTGCAACTTG
AAGCCTGCCG
GAAGGCGAGC
TTCCTGAAGC
AGGAACCCGC
GGATGAGTTT
TCAGAGCTCT
TTGGGCCTCA
CCAGCAGGGC
CTGCCGCCCC
CCTATCCCCT
GTCTCAGTTG
CCGCCTGGCC
CAAGCCTTGG
AGGCCTGGGG
CTGGGCCTGG
CAGGCAGGGT
GGTGGCCGGG
CGGCAGGCGT
GCCGCTGGGT
GGACTGCTGT
GCAGCCTATG
AGCAGCAGGA
GGAGCTGGTG
CGGCACATCG
AGAAGAGCCA
CATCGACCAG
CGCAAGGGCG
AGGACTTCAC
CTGCTTCTGG
GCTGGCTGCG
TGCGCCGCTA
CAAGCCCTTC
AACGCCCGCT
ACAAGCTGCT
CATCCACATG
CGAGTGCACT
CGGGCGAGAA
GCCCAACAAG
TGCATGTTTG
AAGGCTGCAG
CAAGGCCTTC
TCACGGCTGG
AGAACCTCAA
GATCCACCTG
AGGAGCCACA
CGGGCGAGAA
GCCGTACCTG
TGCCAGCACC
CGGGTTGCCA
GAAGGCCTTC
AGCAACTCCA
GCGACCGCGC
CAAGCACCAG
CGCACCCACC
TAGACACGAA
GCCGTACGCC
TGTCAGATCC
CTGGCTGCTC
CAAGCGCTAC
ACAGACCCCA
GCTCCCTCCG
CAAGCACGTC
AAGGCCCATT
CAGCCAAAGA
GCAGCAGGTG
CGTAAGAAGC
TGCATGCGGG
CCCTGACACC
GAGGCCGACG
TCCTGACCGA
GTGTCTGGTC
CTGCAGCAGC
TCCACACGTC
CACACAGCTG
GCTGCCAGCG
ACGGCAAGGG
TGGCTGTGGC
CTGGGCCAGG
AGCTGCTCCC
AGGTGTGTAT
CCTGGCTCCA
TCACCCCCCA
TAACGGACTT
GCATCGGGCC
TCCTGCCCCC
AGCGCACGAC
GTACCTTCCA
GGCACCACCC
GCTGGATGCC
ACCACCAGTT
CCCACCACCA
TCTGTCCCCT
CTGCCCATGG
CTGAGAGCAC
CCGGGATGGG
TTGGGGCCCG
GCCTCCTCTC
ACCAATAGTC
AGCCCCCTGA
AGGGGCTGGG
GCCACCGCCG
CTGCCCCCAT
CCTCTCAGAG
CCATTCTCCG
GGGGGCCAGC
CCTTCCCCAC
ACTCCCCAGC
AAGCCGTCCT
ACCCACCCTT
CCAGAGCCCT
CCACCCCCGC
CTCTGCCCAG
CCCACAAGGT
TACCAGGGCA
GTTTCCACTC
CATCCAGAGT
TGCTTCCCCT
ATGGCGACTG
CTACCGGATG
GCTGAACCAG
CAGCCGGTGG
GGACGGACTG
GTCGGGGAGA
CCCACGGTTT
CAACCCCCTG
CGGCCCAATG
GCTACCACAG
CCTCAGCACG
CCCTTGCCTG
CCACAGGCTA
TGAGGCCCTG
GCTGAGGCCT
CATGCCCCAC
AGCGCTGCCA
CAGCAGCCAT
CTGAAGATGT
GGTGTCCAGC
GGCCCCGAGG
ACTGTGGCTT
CTTCCCCAAT
GGAGCCTTTG
ACCACTGCCT
GGGCCACATC
CCCTCCATCT
ACACAGACAC
CTGAGCGGCC
GCAACTAACT
TAAGCTAGCA
ACGGTTTCCC
TCTAGCGGGA
TCAATTCCGC
CCCCCCCCCC
TAACGTTACT
GGCCGAAGCC
GCTTGGAATA
AGGCCGGTGT
GCGTTTGTCT
ATATGTTATT
TTCCACCATA
TTGCCGTCTT
TTGGCAATGT
GAGGGCCCGG
AAACCTGGCC
CTGTCTTCTT
GACGAGCATT
CCTAGGGGTC
TTTCCCCTCT
CGCCAAAGGA
ATGCAAGGTC
TGTTGAATGT
CGTGAAGGAA
GCAGTTCCTC
TGGAAGCTTC
TTGAAGACAA
ACAACGTCTG
TAGCGACCCT
TTGCAGGCAG
CGGAACCCCC
CACCTGGCGA
CAGGTGCCTC
TGCGGCCAAA
AGCCACGTGT
ATAAGATACA
CCTGCAAAGG
CGGCACAACC
CCAGTGCCAC
GTTGTGAGTT
GGATAGTTGT
GGAAAGAGTC
AAATGGCTCT
CCTCAAGCGT
ATTCAACAAG
GGGCTGAAGG
ATGCCCAGAA
GGTACCCCAT
TGTATGGGAT
CTGATCTGGG
GCCTCGGTGC
ACATGCTTTA
CATGTGTTTA
GTCGAGGTTA
AAAAAACGTC
TAGGCCCCCC
GAACCACGGG
GACGTGGTTT
TCCTTTGAAA
AACACGATAA
TACCATGACC
GAGTACAAGC
CCACGGTGCG
CCTCGCCACC
CGCGACGACG
TCCCCAGGGC
CGTACGCACC
CTCGCCGCCG
CGTTCGCCGA
CTACCCCGCC
ACGCGCCACA
CCGTCGATCC
GGACCGCCAC
ATCGAGCGGG
TCACCGAGCT
GCAAGAACTC
TTCCTCACGC
GCGTCGGGCT
CGACATCGGC
AAGGTGTGGG
TCGCGGACGA
CGGCGCCGCG
GTGGCGGTCT
GGACCACGCC
GGAGAGCGTC
GAAGCGGGGG
CGGTGTTCGC
CGAGATCGGC
CCGCGCATGG
CCGAGTTGAG
CGGTTCCCGG
CTGGCCGCGC
AGCAACAGAT
GGAAGGCCTC
CTGGCGCCGC
ACCGGCCCAA
GGAGCCCGCG
TGGTTCCTGG
CCACCGTCGG
CGTCTCGCCC
GACCACCAGG
GCAAGGGTCT
GGGCAGCGCC
GTCGTGCTCC
CCGGAGTGGA
GGCGGCCGAG
CGCGCCGGGG
TGCCCGCCTT
CCTGGAGACC
TCCGCGCCCC
GCAACCTCCC
CTTCTACGAG
CGGCTCGGCT
TCACCGTCAC
CGCCGACGTC
GAGGTGCCCG
AAGGACCGCG
CACCTGGTGC
ATGACCCGCA
AGCCCGGTGC
CTGAGAATTG
GCAAGCTGCT
TACATAGAAC
TCGCGGCGAT
TGGCATGCCG
CCTTAAAATT
TTTATTTTAT
TTTTCTTTTC
TTTTCCGAAT
CGGATTTTGT
TTTTAATATT
TCAAAAAAAA
AAAAAAAAAA
AAAAAAAACG
CGTCGAGGGG
AATTAATTCT
TGAAGACGAA
AGGGCCAGGT
GGCACTTTTC
GGGGAAATGT
GCGCGGAACC
CCTATTTGTT
TATTTTTCTA
AATACATTCA
AATATGTATC
CGCTCATGAG
ACAATAACCC
TGATAAATGC
TTCAATAATA
TTGAAAAAGG
AAGAGTATGA
GTATTCAACA
TTTCCGTGTC
GCCCTTATTC
CCTTTTTTGC
GGCATTTTGC
CTTCCTGTTT
TTGCTCACCC
AGAAACGCTG
GTGAAAGTAA
AAGATGCTGA
AGATCAGTTG
GGTGCACGAG
TGGGTTACAT
CGAACTGGAT
CTCAACAGCG
GTAAGATCCT
TGAGAGTTTT
CGCCCCGAAG
AACGTTTTCC
AATGATGAGC
ACTTTTAAAG
TTCTGCTATG
TGGCGCGGTA
TTATCCCGTG
TTGACGCCGG
GCAAGAGCAA
CTCGGTCGCC
GCATACACTA
TTCTCAGAAT
GACTTGGTTG
AGTACTCACC
AGTCACAGAA
AAGCATCTTA
CGGATGGCAT
GACAGTAAGA
GAATTATGCA
GTGCTGCCAT
AACCATGAGT
GATAACACTG
CGGCCAACTT
ACTTCTGACA
ACGATCGGAG
GACCGAAGGA
GCTAACCGCT
TTTTTGCACA
ACATGGGGGA
TCATGTAACT
CGCCTTGATC
GTTGGGAACC
GGAGCTGAAT
GAAGCCATAC
CAAACGACGA
GCGTGACACC
ACGATGCCTG
TAGCAATGGC
AACAACGTTG
CGCAAACTAT
TAACTGGCGA
ACTACTTACT
CTAGCTTCCC
GGCAACAATT
AATAGACTGG
ATGGAGGCGG
ATAAAGTTGC
AGGACCACTT
CTGCGCTCGG
CCCTTCCGGC
TGGCTGGTTT
ATTGCTGATA
AATCTGGAGC
CGGTGAGCGT
GGGTCTCGCG
GTATCATTGC
AGCACTGGGG
CCAGATGGTA
AGCCCTCCCG
TATCGTAGTT
ATCTACACGA
CGGGGAGTCA
GGCAACTATG
GATGAACGAA
ATAGACAGAT
CGCTGAGATA
GGTGCCTCAC
TGATTAAGCA
TTGGTAACTG
TCAGACCAAG
TTTACTCATA
TATACTTTAG
ATTGATTTAA
AACTTCATTT
TTAATTTAAA
AGGATCTAGG
TGAAGATCCT
TTTTGATAAT
CTCATGACCA
AAATCCCTTA
ACGTGAGTTT
TCGTTCCACT
GAGCGTCAGA
CCCCGTAGAA
AAGATCAAAG
GATCTTCTTG
AGATCCTTTT
TTTCTGCGCG
TAATCTGCTG
CTTGCAAACA
AAAAAACCAC
CGCTACCAGC
GGTGGTTTGT
TTGCCGGATC
AAGAGCTACC
AACTCTTTTT
CCGAAGGTAA
CTGGCTTCAG
CAGAGCGCAG
ATACCAAATA
CTGTCCTTCT
AGTGTAGCCG
TAGTTAGGCC
ACCACTTCAA
GAACTCTGTA
GCACCGCCTA
CATACCTCGC
TCTGCTAATC
CTGTTACCAG
TGGCTGCTGC
CAGTGGCGAT
AAGTCGTGTC
TTACCGGGTT
GGACTCAAGA
CGATAGTTAC
CGGATAAGGC
GCAGCGGTCG
GGCTGAACGG
GGGGTTCGTG
CACACAGCCC
AGCTTGGAGC
GAACGACCTA
CACCGAACTG
AGATACCTAC
AGCGTGAGCA
TTGAGAAAGC
GCCACGCTTC
CCGAAGGGAG
AAAGGCGGAC
AGGTATCCGG
TAAGCGGCAG
GGTCGGAACA
GGAGAGCGCA
CGAGGGAGCT
TCCAGGGGGA
AACGCCTGGT
ATCTTTATAG
TCCTGTCGGG
TTTCGCCACC
TCTGACTTGA
GCGTCGATTT
TTGTGATGCT
CGTCAGGGGG
GCGGAGCCTA
TGGAAAAACG
CCAGCAACGC
GAGCTCTAAT
ACGACTCACT
ATA