名称: | pTX172-BRI1-sgRNA1-UBI-Cas9 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | Hyg |
荧光标记: | |
启动子: | UBI |
复制子: | pVS1 |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGATATTCTT
GGAGTAGACG
AGAGTGTCGT
GCTCCACCAT
GGGCGCGCCT
GCAGTGCAGC
GTGACCCGGT
CGTGCCCCTC
TCTTGAGATA
AGGTGAGCAT
TGCATGTCTA
AGTTATAAAA
AATTACCACA
TATTTTTTTT
GTCACACTTG
TTTGAAGTGC
AGTTTATCTA
TCTTTATACA
TATATTTAAA
CTTTACTCTA
CGAATAATAT
AATCTATAGT
ACTACAATAA
TATCAGTGTT
TTAGAGAATC
ATATAAATGA
ACAGTTAGAC
ATGGTCTAAA
GGACAATTGA
GTATTTTGAC
AACAGGACTC
TACAGTTTTA
TCTTTTTAGT
GTGCATGTGT
TCTCCTTTTT
TTTTGCAAAT
AGCTTCACCT
ATATAATACT
TCATCCATTT
TATTAGTACA
TCCATTTAGG
GTTTAGGGTT
AATGGTTTTT
ATAGACTAAT
TTTTTTAGTA
CATCTATTTT
ATTCTATTTT
AGCCTCTAAA
TTAAGAAAAC
TAAAACTCTA
TTTTAGTTTT
TTTATTTAAT
AATTTAGATA
TAAAATAGAA
TAAAATAAAG
TGACTAAAAA
TTAAACAAAT
ACCCTTTAAG
AAATTAAAAA
AACTAAGGAA
ACATTTTTCT
TGTTTCGAGT
AGATAATGCC
AGCCTGTTAA
ACGCCGTCGA
CGAGTCTAAC
GGACACCAAC
CAGCGAACCA
GCAGCGTCGC
GTCGGGCCAA
GCGAAGCAGA
CGGCACGGCA
TCTCTGTCGC
TGCCTCTGGA
CCCCTCTCGA
GAGTTCCGCT
CCACCGTTGG
ACTTGCTCCG
CTGTCGGCAT
CCAGAAATTG
CGTGGCGGAG
CGGCAGACGT
GAGCCGGCAC
GGCAGGCGGC
CTCCTCCTCC
TCTCACGGCA
CCGGCAGCTA
CGGGGGATTC
CTTTCCCACC
GCTCCTTCGC
TTTCCCTTCC
TCGCCCGCCG
TAATAAATAG
ACACCCCCTC
CACACCCTCT
TTCCCCAACC
TCGTGTTGTT
CGGAGCGCAC
ACACACACAA
CCAGAACTCC
CCCAAATCCA
CCCGTCGGCA
CCTCCGCTTC
AAGGTACGCC
GCTCGTCCTC
CCCCCCCCCC
TCTCTACCTT
CTCAAGATCG
GCGTTCCGGT
CCATGGTTAG
GGCCCGGTAG
TTCTACTTCT
GTTCATGTTT
GTGTTAGATC
CGTGTTTGTG
TTAGATCCGT
GCTACTAGCG
TTCGTACACG
GATGCGACCT
GTACGTCAGA
CACGTTCTGA
TTGCTAACTT
GCCAGTGTTT
CTCTTTGGGG
AATCCTGGGA
TGGCTCTAGC
CGTTCCGCAG
ACGGGATCGA
TTTCATGATT
TTTTTTGTTT
CGTTGCATAG
GGTTTGGTTT
GCCCTTTTCC
TTTATTTCAA
TATATGCCGT
GCACTTGTTT
GTCGGGTCAT
CTTTTCATGC
TTTTTTTTGT
CTTGGTTGTG
ATGATGTGGT
CTGGTTGGGC
GGTCGTTCAA
GATCGGAGTA
GAATTAATTC
TGTTTCAAAC
TACCTGGTGG
ATTTATTAAT
TTTGGATCTG
TATGTGTGTG
CCATACATAT
TCATAGTTAC
GAATTGAAGA
TGATGGATGG
AAATATCGAT
CTAGGATAGG
TATACATGTT
GATGCGGGTT
TTACTGATGC
ATATACAGAG
ATGCTTTTTG
TTCGCTTGGT
TGTGATGATG
TGGTGTGGTT
GGGCGGTCGT
TCATTCGTTC
AAGATCGGAG
TAGAATACTG
TTTCAAACTA
CCTGGTGTAT
TTATTAATTT
TGGAACTGTA
TGTGTGTGTC
ATACATCTTC
ATAGTTACGA
GTTTAAGATG
GATGGAAATA
TCGATCTAGG
ATAGGTATAC
ATGTTGATGT
GGGTTTTACT
GATGCATATA
CATGATGGCA
TATGCAGCAT
CTATTCATAT
GCTCTAACCT
TGAGTACCTA
TCTATTATAA
TAAACAAGTA
TGTTTTATAA
TTATTTTGAT
CTTGATATAC
TTGGATGATG
GCATATGCAG
CAGCTATATG
TGGATTTTTT
TAGCCCTGCC
TTCATACGCT
ATTTATTTGC
TTGGTACTGT
TTCTTTTGTC
GATGCTCACC
CTGTTGTTTG
GTGTTACTTC
TGCAGCCTGC
AGGTAGATCG
CTCGTCGACA
TGGATAAGAA
GTACTCTATC
GGACTCGATA
TCGGAACTAA
CTCTGTGGGA
TGGGCTGTGA
TCACCGATGA
GTACAAGGTG
CCATCTAAGA
AGTTCAAGGT
TCTCGGAAAC
ACCGATAGGC
ACTCTATCAA
GAAAAACCTT
ATCGGTGCTC
TCCTCTTCGA
TTCTGGTGAA
ACTGCTGAGG
CTACCAGACT
CAAGAGAACC
GCTAGAAGAA
GGTACACCAG
AAGAAAGAAC
AGGATCTGCT
ACCTCCAAGA
GATCTTCTCT
AACGAGATGG
CTAAAGTGGA
TGATTCATTC
TTCCACAGGC
TCGAAGAGTC
ATTCCTCGTG
GAAGAAGATA
AGAAGCACGA
GAGGCACCCT
ATCTTCGGAA
ACATCGTTGA
TGAGGTGGCA
TACCACGAGA
AGTACCCTAC
TATCTACCAC
CTCAGAAAGA
AGCTCGTTGA
TTCTACTGAT
AAGGCTGATC
TCAGGCTCAT
CTACCTCGCT
CTCGCTCACA
TGATCAAGTT
CAGAGGACAC
TTCCTCATCG
AGGGTGATCT
CAACCCTGAT
AACTCTGATG
TGGATAAGTT
GTTCATCCAG
CTCGTGCAGA
CCTACAACCA
GCTTTTCGAA
GAGAACCCTA
TCAACGCTTC
AGGTGTGGAT
GCTAAGGCTA
TCCTCTCTGC
TAGGCTCTCT
AAGTCAAGAA
GGCTTGAGAA
CCTCATTGCT
CAGCTCCCTG
GTGAGAAGAA
GAACGGACTT
TTCGGAAACT
TGATCGCTCT
CTCTCTCGGA
CTCACCCCTA
ACTTCAAGTC
TAACTTCGAT
CTCGCTGAGG
ATGCAAAGCT
CCAGCTCTCA
AAGGATACCT
ACGATGATGA
TCTCGATAAC
CTCCTCGCTC
AGATCGGAGA
TCAGTACGCT
GATTTGTTCC
TCGCTGCTAA
GAACCTCTCT
GATGCTATCC
TCCTCAGTGA
TATCCTCAGA
GTGAACACCG
AGATCACCAA
GGCTCCACTC
TCAGCTTCTA
TGATCAAGAG
ATACGATGAG
CACCACCAGG
ATCTCACACT
TCTCAAGGCT
CTTGTTAGAC
AGCAGCTCCC
AGAGAAGTAC
AAAGAGATTT
TCTTCGATCA
GTCTAAGAAC
GGATACGCTG
GTTACATCGA
TGGTGGTGCA
TCTCAAGAAG
AGTTCTACAA
GTTCATCAAG
CCTATCCTCG
AGAAGATGGA
TGGAACCGAG
GAACTCCTCG
TGAAGCTCAA
TAGAGAGGAT
CTTCTCAGAA
AGCAGAGGAC
CTTCGATAAC
GGATCTATCC
CTCATCAGAT
CCACCTCGGA
GAGTTGCACG
CTATCCTTAG
AAGGCAAGAG
GATTTCTACC
CATTCCTCAA
GGATAACAGG
GAAAAGATTG
AGAAGATTCT
CACCTTCAGA
ATCCCTTACT
ACGTGGGACC
TCTCGCTAGA
GGAAACTCAA
GATTCGCTTG
GATGACCAGA
AAGTCTGAGG
AAACCATCAC
CCCTTGGAAC
TTCGAAGAGG
TGGTGGATAA
GGGTGCTAGT
GCTCAGTCTT
TCATCGAGAG
GATGACCAAC
TTCGATAAGA
ACCTTCCAAA
CGAGAAGGTG
CTCCCTAAGC
ACTCTTTGCT
CTACGAGTAC
TTCACCGTGT
ACAACGAGTT
GACCAAGGTT
AAGTACGTGA
CCGAGGGAAT
GAGGAAGCCT
GCTTTTTTGT
CAGGTGAGCA
AAAGAAGGCT
ATCGTTGATC
TCTTGTTCAA
GACCAACAGA
AAGGTGACCG
TGAAGCAGCT
CAAAGAGGAT
TACTTCAAGA
AAATCGAGTG
CTTCGATTCA
GTTGAGATTT
CTGGTGTTGA
GGATAGGTTC
AACGCATCTC
TCGGAACCTA
CCACGATCTC
CTCAAGATCA
TTAAGGATAA
GGATTTCTTG
GATAACGAGG
AAAACGAGGA
TATCTTGGAG
GATATCGTTC
TTACCCTCAC
CCTCTTTGAA
GATAGAGAGA
TGATTGAAGA
AAGGCTCAAG
ACCTACGCTC
ATCTCTTCGA
TGATAAGGTG
ATGAAGCAGT
TGAAGAGAAG
AAGATACACT
GGTTGGGGAA
GGCTCTCAAG
AAAGCTCATT
AACGGAATCA
GGGATAAGCA
GTCTGGAAAG
ACAATCCTTG
ATTTCCTCAA
GTCTGATGGA
TTCGCTAACA
GAAACTTCAT
GCAGCTCATC
CACGATGATT
CTCTCACCTT
TAAAGAGGAT
ATCCAGAAGG
CTCAGGTTTC
AGGACAGGGT
GATAGTCTCC
ATGAGCATAT
CGCTAACCTC
GCTGGATCTC
CTGCAATCAA
GAAGGGAATC
CTCCAGACTG
TGAAGGTTGT
GGATGAGTTG
GTGAAGGTGA
TGGGAAGGCA
TAAGCCTGAG
AACATCGTGA
TCGAAATGGC
TAGAGAGAAC
CAGACCACTC
AGAAGGGACA
GAAGAACTCT
AGGGAAAGGA
TGAAGAGGAT
CGAGGAAGGT
ATCAAAGAGC
TTGGATCTCA
GATCCTCAAA
GAGCACCCTG
TTGAGAACAC
TCAGCTCCAG
AATGAGAAGC
TCTACCTCTA
CTACCTCCAG
AACGGAAGGG
ATATGTATGT
GGATCAAGAG
TTGGATATCA
ACAGGCTCTC
TGATTACGAT
GTTGATCATA
TCGTGCCACA
GTCATTCTTG
AAGGATGATT
CTATCGATAA
CAAGGTGCTC
ACCAGGTCTG
ATAAGAACAG
GGGTAAGAGT
GATAACGTGC
CAAGTGAAGA
GGTTGTGAAG
AAAATGAAGA
ACTATTGGAG
GCAGCTCCTC
AACGCTAAGC
TCATCACTCA
GAGAAAGTTC
GATAACTTGA
CTAAGGCTGA
GAGGGGAGGA
CTCTCTGAAT
TGGATAAGGC
AGGATTCATC
AAGAGGCAGC
TTGTGGAAAC
CAGGCAGATC
ACTAAGCACG
TTGCACAGAT
CCTCGATTCT
AGGATGAACA
CCAAGTACGA
TGAGAACGAT
AAGTTGATCA
GGGAAGTGAA
GGTTATCACC
CTCAAGTCAA
AGCTCGTGTC
TGATTTCAGA
AAGGATTTCC
AATTCTACAA
GGTGAGGGAA
ATCAACAACT
ACCACCACGC
TCACGATGCT
TACCTTAACG
CTGTTGTTGG
AACCGCTCTC
ATCAAGAAGT
ATCCTAAGCT
CGAGTCAGAG
TTCGTGTACG
GTGATTACAA
GGTGTACGAT
GTGAGGAAGA
TGATCGCTAA
GTCTGAGCAA
GAGATCGGAA
AGGCTACCGC
TAAGTATTTC
TTCTACTCTA
ACATCATGAA
TTTCTTCAAG
ACCGAGATTA
CCCTCGCTAA
CGGTGAGATC
AGAAAGAGGC
CACTCATCGA
GACAAACGGT
GAAACAGGTG
AGATCGTGTG
GGATAAGGGA
AGGGATTTCG
CTACCGTTAG
AAAGGTGCTC
TCTATGCCAC
AGGTGAACAT
CGTTAAGAAA
ACCGAGGTGC
AGACCGGTGG
ATTCTCTAAA
GAGTCTATCC
TCCCTAAGAG
GAACTCTGAT
AAGCTCATTG
CTAGGAAGAA
GGATTGGGAC
CCTAAGAAAT
ACGGTGGTTT
CGATTCTCCT
ACCGTGGCTT
ACTCTGTTCT
CGTTGTGGCT
AAGGTTGAGA
AGGGAAAGAG
TAAGAAGCTC
AAGTCTGTTA
AGGAACTTCT
CGGAATCACT
ATCATGGAAA
GGTCATCTTT
CGAGAAGAAC
CCAATCGATT
TCCTCGAGGC
TAAGGGATAC
AAAGAGGTTA
AGAAGGATCT
CATCATCAAG
CTCCCAAAGT
ACTCACTCTT
CGAACTCGAG
AACGGTAGAA
AGAGGATGCT
CGCTTCTGCT
GGTGAGCTTC
AAAAGGGAAA
CGAGCTTGCT
CTCCCATCTA
AGTACGTTAA
CTTTCTTTAC
CTCGCTTCTC
ACTACGAGAA
GTTGAAGGGA
TCTCCAGAAG
ATAACGAGCA
GAAGCAACTT
TTCGTTGAGC
AGCACAAGCA
CTACTTGGAT
GAGATCATCG
AGCAGATCTC
TGAGTTCTCT
AAAAGGGTGA
TCCTCGCTGA
TGCAAACCTC
GATAAGGTGT
TGTCTGCTTA
CAACAAGCAC
AGAGATAAGC
CTATCAGGGA
ACAGGCAGAG
AACATCATCC
ATCTCTTCAC
CCTTACCAAC
CTCGGTGCTC
CTGCTGCTTT
CAAGTACTTC
GATACAACCA
TCGATAGGAA
GAGATACACC
TCTACCAAAG
AAGTGCTCGA
TGCTACCCTC
ATCCATCAGT
CTATCACTGG
ACTCTACGAG
ACTAGGATCG
ATCTCTCACA
GCTCGGTGGT
GATTCAAGGG
CTGATCCTAA
GAAGAAGAGG
AAGGTTTGAG
GATCTAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAAAAAA
AAAAAGGCTT
TTGTCACCGG
ATTGGGTCAT
AACGATATCT
CGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTATCAA
CTTGAAAAAG
TGGCACCGAG
TCGGTGCTTT
TGTCACCGGA
TGTGTTTTCC
GGTCTGATGA
GTCCGTGAGG
ACGAAACAAA
AGCGAGCTCA
AACTATCAGT
GTTTGACAGG
ATATATTGGC
GGGTAAACCT
AAGAGAAAAG
AGCGTTTATT
AGAATAATCG
GATATTTAAA
AGGGCGTGAA
AAGGTTTATC
CGTTCGTCCA
TTTGTATGTG
CATGCCAACC
ACAGGGTTCC
CCTCGGGATC
AAAGTACTTT
GATCCAACCC
CTCCGCTGCT
ATAGTGCAGT
CGGCTTCTGA
CGTTCAGTGC
AGCCGTCTTC
TGAAAACGAC
ATGTCGCACA
AGTCCTAAGT
TACGCGACAG
GCTGCCGCCC
TGCCCTTTTC
CTGGCGTTTT
CTTGTCGCGT
GTTTTAGTCG
CATAAAGTAG
AATACTTGCG
ACTAGAACCG
GAGACATTAC
GCCATGAACA
AGAGCGCCGC
CGCTGGCCTG
CTGGGCTATG
CCCGCGTCAG
CACCGACGAC
CAGGACTTGA
CCAACCAACG
GGCCGAACTG
CACGCGGCCG
GCTGCACCAA
GCTGTTTTCC
GAGAAGATCA
CCGGCACCAG
GCGCGACCGC
CCGGAGCTGG
CCAGGATGCT
TGACCACCTA
CGCCCTGGCG
ACGTTGTGAC
AGTGACCAGG
CTAGACCGCC
TGGCCCGCAG
CACCCGCGAC
CTACTGGACA
TTGCCGAGCG
CATCCAGGAG
GCCGGCGCGG
GCCTGCGTAG
CCTGGCAGAG
CCGTGGGCCG
ACACCACCAC
GCCGGCCGGC
CGCATGGTGT
TGACCGTGTT
CGCCGGCATT
GCCGAGTTCG
AGCGTTCCCT
AATCATCGAC
CGCACCCGGA
GCGGGCGCGA
GGCCGCCAAG
GCCCGAGGCG
TGAAGTTTGG
CCCCCGCCCT
ACCCTCACCC
CGGCACAGAT
CGCGCACGCC
CGCGAGCTGA
TCGACCAGGA
AGGCCGCACC
GTGAAAGAGG
CGGCTGCACT
GCTTGGCGTG
CATCGCTCGA
CCCTGTACCG
CGCACTTGAG
CGCAGCGAGG
AAGTGACGCC
CACCGAGGCC
AGGCGGCGCG
GTGCCTTCCG
TGAGGACGCA
TTGACCGAGG
CCGACGCCCT
GGCGGCCGCC
GAGAATGAAC
GCCAAGAGGA
ACAAGCATGA
AACCGCACCA
GGACGGCCAG
GACGAACCGT
TTTTCATTAC
CGAAGAGATC
GAGGCGGAGA
TGATCGCGGC
CGGGTACGTG
TTCGAGCCGC
CCGCGCACGG
CTCAACCGTG
CGGCTGCATG
AAATCCTGGC
CGGTTTGTCT
GATGCCAAGC
TGGCGGCCTG
GCCGGCCAGC
TTGGCCGCTG
AAGAAACCGA
GCGCCGCCGT
CTAAAAAGGT
GATGTGTATT
TGAGTAAAAC
AGCTTGCGTC
ATGCGGTCGC
TGCGTATATG
ATGCGATGAG
TAAATAAACA
AATACGCAAG
GGGAACGCAT
GAAGGTTATC
GCTGTACTTA
ACCAGAAAGG
CGGGTCAGGC
AAGACGACCA
TCGCAACCCA
TCTAGCCCGC
GCCCTGCAAC
TCGCCGGGGC
CGATGTTCTG
TTAGTCGATT
CCGATCCCCA
GGGCAGTGCC
CGCGATTGGG
CGGCCGTGCG
GGAAGATCAA
CCGCTAACCG
TTGTCGGCAT
CGACCGCCCG
ACGATTGACC
GCGACGTGAA
GGCCATCGGC
CGGCGCGACT
TCGTAGTGAT
CGACGGAGCG
CCCCAGGCGG
CGGACTTGGC
TGTGTCCGCG
ATCAAGGCAG
CCGACTTCGT
GCTGATTCCG
GTGCAGCCAA
GCCCTTACGA
CATATGGGCC
ACCGCCGACC
TGGTGGAGCT
GGTTAAGCAG
CGCATTGAGG
TCACGGATGG
AAGGCTACAA
GCGGCCTTTG
TCGTGTCGCG
GGCGATCAAA
GGCACGCGCA
TCGGCGGTGA
GGTTGCCGAG
GCGCTGGCCG
GGTACGAGCT
GCCCATTCTT
GAGTCCCGTA
TCACGCAGCG
CGTGAGCTAC
CCAGGCACTG
CCGCCGCCGG
CACAACCGTT
CTTGAATCAG
AACCCGAGGG
CGACGCTGCC
CGCGAGGTCC
AGGCGCTGGC
CGCTGAAATT
AAATCAAAAC
TCATTTGAGT
TAATGAGGTA
AAGAGAAAAT
GAGCAAAAGC
ACAAACACGC
TAAGTGCCGG
CCGTCCGAGC
GCACGCAGCA
GCAAGGCTGC
AACGTTGGCC
AGCCTGGCAG
ACACGCCAGC
CATGAAGCGG
GTCAACTTTC
AGTTGCCGGC
GGAGGATCAC
ACCAAGCTGA
AGATGTACGC
GGTACGCCAA
GGCAAGACCA
TTACCGAGCT
GCTATCTGAA
TACATCGCGC
AGCTACCAGA
GTAAATGAGC
AAATGAATAA
ATGAGTAGAT
GAATTTTAGC
GGCTAAAGGA
GGCGGCATGG
AAAATCAAGA
ACAACCAGGC
ACCGACGCCG
TGGAATGCCC
CATGTGTGGA
GGAACGGGCG
GTTGGCCAGG
CGTAAGCGGC
TGGGTTGCCT
GCCGGCCCTG
CAATGGCACT
GGAACCCCCA
AGCCCGAGGA
ATCGGCGTGA
GCGGTCGCAA
ACCATCCGGC
CCGGTACAAA
TCGGCGCGGC
GCTGGGTGAT
GACCTGGTGG
AGAAGTTGAA
GGCCGCGCAG
GCCGCCCAGC
GGCAACGCAT
CGAGGCAGAA
GCACGCCCCG
GTGAATCGTG
GCAAGCGGCC
GCTGATCGAA
TCCGCAAAGA
ATCCCGGCAA
CCGCCGGCAG
CCGGTGCGCC
GTCGATTAGG
AAGCCGCCCA
AGGGCGACGA
GCAACCAGAT
TTTTTCGTTC
CGATGCTCTA
TGACGTGGGC
ACCCGCGATA
GTCGCAGCAT
CATGGACGTG
GCCGTTTTCC
GTCTGTCGAA
GCGTGACCGA
CGAGCTGGCG
AGGTGATCCG
CTACGAGCTT
CCAGACGGGC
ACGTAGAGGT
TTCCGCAGGG
CCGGCCGGCA
TGGCCAGTGT
GTGGGATTAC
GACCTGGTAC
TGATGGCGGT
TTCCCATCTA
ACCGAATCCA
TGAACCGATA
CCGGGAAGGG
AAGGGAGACA
AGCCCGGCCG
CGTGTTCCGT
CCACACGTTG
CGGACGTACT
CAAGTTCTGC
CGGCGAGCCG
ATGGCGGAAA
GCAGAAAGAC
GACCTGGTAG
AAACCTGCAT
TCGGTTAAAC
ACCACGCACG
TTGCCATGCA
GCGTACGAAG
AAGGCCAAGA
ACGGCCGCCT
GGTGACGGTA
TCCGAGGGTG
AAGCCTTGAT
TAGCCGCTAC
AAGATCGTAA
AGAGCGAAAC
CGGGCGGCCG
GAGTACATCG
AGATCGAGCT
AGCTGATTGG
ATGTACCGCG
AGATCACAGA
AGGCAAGAAC
CCGGACGTGC
TGACGGTTCA
CCCCGATTAC
TTTTTGATCG
ATCCCGGCAT
CGGCCGTTTT
CTCTACCGCC
TGGCACGCCG
CGCCGCAGGC
AAGGCAGAAG
CCAGATGGTT
GTTCAAGACG
ATCTACGAAC
GCAGTGGCAG
CGCCGGAGAG
TTCAAGAAGT
TCTGTTTCAC
CGTGCGCAAG
CTGATCGGGT
CAAATGACCT
GCCGGAGTAC
GATTTGAAGG
AGGAGGCGGG
GCAGGCTGGC
CCGATCCTAG
TCATGCGCTA
CCGCAACCTG
ATCGAGGGCG
AAGCATCCGC
CGGTTCCTAA
TGTACGGAGC
AGATGCTAGG
GCAAATTGCC
CTAGCAGGGG
AAAAAGGTCG
AAAAGGCCTC
TTTCCTGTGG
ATAGCACGTA
CATTGGGAAC
CCAAAGCCGT
ACATTGGGAA
CCGGAACCCG
TACATTGGGA
ACCCAAAGCC
GTACATTGGG
AACCGGTCAC
ACATGTAAGT
GACTGATATA
AAAGAGAAAA
AAGGCGATTT
TTCCGCCTAA
AACTCTTTAA
AACTTATTAA
AACTCTTAAA
ACCCGCCTGG
CCTGTGCATA
ACTGTCTGGC
CAGCGCACAG
CCGAAGAGCT
GCAAAAAGCG
CCTACCCTTC
GGTCGCTGCG
CTCCCTACGC
CCCGCCGCTT
CGCGTCGGCC
TATCGCGGCC
GCTGGCCGCT
CAAAAATGGC
TGGCCTACGG
CCAGGCAATC
TACCAGGGCG
CGGACAAGCC
GCGCCGTCGC
CACTCGACCG
CCGGCGCCCA
CATCAAGGCA
CCCTGCCTCG
CGCGTTTCGG
TGATGACGGT
GAAAACCTCT
GACACATGCA
GCTCCCGGAA
ACGGTCACAG
CTTGTCTGTA
AGCGGATGCC
GGGAGCAGAC
AAGCGCGTCA
GCGGGTGTTG
GCGGGTGTCG
GGGCGCAGCC
ATGACCCAGT
CACGTAGCGA
TAGCGGAGTG
TATACTGGCT
TAACTATGCG
GCATCAGAGC
AGATTGTACT
GAGAGTGCAC
CATATGCGGT
GTGAAATACC
GCACAGATGC
GTAAGGAGAA
AATACCGCAT
CAGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
GACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGCATTCTA
GGTACTAAAA
CAATTCATCC
AGTAAAATAT
AATATTTTAT
TTTCTCCCAA
TCAGGCTTGA
TCCCCAGTAA
GTCAAAAAAT
AGCTCGACAT
ACTGTTCTTC
CCCGATATCC
TCCCTGATCG
ACCGGACGCA
GAAGGCAATG
TCATACCACT
TGTCCGCCCT
GCCGCTTCTC
CCAAGATCAA
TAAAGCCACT
TACTTTGCCA
TCTTTCACAA
AGATGTTGCT
GTCTCCCAGG
TCGCCGTGGG
AAAAGACAAG
TTCCTCTTCG
GGCTTTTCCG
TCTTTAAAAA
ATCATACAGC
TCGCGCGGAT
CTTTAAATGG
AGTGTCTTCT
TCCCAGTTTT
CGCAATCCAC
ATCGGCCAGA
TCGTTATTCA
GTAAGTAATC
CAATTCGGCT
AAGCGGCTGT
CTAAGCTATT
CGTATAGGGA
CAATCCGATA
TGTCGATGGA
GTGAAAGAGC
CTGATGCACT
CCGCATACAG
CTCGATAATC
TTTTCAGGGC
TTTGTTCATC
TTCATACTCT
TCCGAGCAAA
GGACGCCATC
GGCCTCACTC
ATGAGCAGAT
TGCTCCAGCC
ATCATGCCGT
TCAAAGTGCA
GGACCTTTGG
AACAGGCAGC
TTTCCTTCCA
GCCATAGCAT
CATGTCCTTT
TCCCGTTCCA
CATCATAGGT
GGTCCCTTTA
TACCGGCTGT
CCGTCATTTT
TAAATATAGG
TTTTCATTTT
CTCCCACCAG
CTTATATACC
TTAGCAGGAG
ACATTCCTTC
CGTATCTTTT
ACGCAGCGGT
ATTTTTCGAT
CAGTTTTTTC
AATTCCGGTG
ATATTCTCAT
TTTAGCCATT
TATTATTTCC
TTCCTCTTTT
CTACAGTATT
TAAAGATACC
CCAAGAAGCT
AATTATAACA
AGACGAACTC
CAATTCACTG
TTCCTTGCAT
TCTAAAACCT
TAAATACCAG
AAAACAGCTT
TTTCAAAGTT
GTTTTCAAAG
TTGGCGTATA
ACATAGTATC
GACGGAGCCG
ATTTTGAAAC
CGCGGTGATC
ACAGGCAGCA
ACGCTCTGTC
ATCGTTACAA
TCAACATGCT
ACCCTCCGCG
AGATCATCCG
TGTTTCAAAC
CCGGCAGCTT
AGTTGCCGTT
CTTCCGAATA
GCATCGGTAA
CATGAGCAAA
GTCTGCCGCC
TTACAACGGC
TCTCCCGCTG
ACGCCGTCCC
GGACTGATGG
GCTGCCTGTA
TCGAGTGGTG
ATTTTGTGCC
GAGCTGCCGG
TCGGGGAGCT
GTTGGCTGGC
TGGTGGCAGG
ATATATTGTG
GTGTAAACAA
ATTGACGCTT
AGACAACTTA
ATAACACATT
GCGGACGTTT
TTAATGTACT
GAATTAACGC
CGAATTAATT
CGGGGGATCT
GGATTTTAGT
ACTGGATTTT
GGTTTTAGGA
ATTAGAAATT
TTATTGATAG
AAGTATTTTA
CAAATACAAA
TACATACTAA
GGGTTTCTTA
TATGCTCAAC
ACATGAGCGA
AACCCTATAG
GAACCCTAAT
TCCCTTATCT
GGGAACTACT
CACACATTAT
TATGGAGAAA
ATAGAGCTTG
TCGATCGTTA
ATTAATCACC
TCCAAGATCC
CGGACGAGTG
CTGGGGCGTC
GGTTTCCACT
ATCGGCGAGT
ACTTCTACAC
AGCCATCGGT
CCAGACGGCC
GCGCTTCTGC
GGGCGATTTG
TGTACGCCCG
ACAGTCCCGG
CTCCGGATCG
GACGATTGCG
TCGCATCGAC
CCTGCGCCCA
AGCTGCATCA
TCGAAATTGC
CGTCAACCAA
GCTCTGATAG
AGTTGGTCAA
GACCAATGCG
GAGCATATAC
GCCCGGAGCC
GCGGCGATCC
TGCAAGCTCC
GGATGCCTCC
GCTCGAAGTA
GCGCGTCTGC
TGCTCCATAC
AAGCCAACCA
CGGCCTCCAG
AAGAAGATGT
TGGCGACCTC
GTATTGGGAA
TCCCCGAACA
TCGCCTCGCT
CCAGTCAATG
ACCGCTGTTA
TGCGGCCATT
GTCCGTCAGG
ACATTGTTGG
AGCCGAAATC
CGCGTGCACG
AGGTGCCGGA
CTTCGGGGCA
GTCCTCGGCC
CAAAGCATCA
GCTCATCGAG
AGCCTGCGCG
ACGGACGCAC
TGACGGTGTC
GTCCATCACA
GTTTGCCAGT
GATACACATG
GGGATCAGCA
ATCGCGCATA
TGAAATCACG
CCATGTAGTG
TATTGACCGA
TTCCTTGCGG
TCCGAATGGG
CCGAACCCGC
TCGTCTGGCT
AAGATCGGCC
GCAGCGATCG
CATCCATGGC
CTCCGCGACC
GGCTGCAGAA
CAGCGGGCAG
TTCGGTTTCA
GGCAGGTCTT
GCAACGTGAC
ACCCTGTGCA
CGGCGGGAGA
TGCAATAGGT
CAGGCTCTCG
CTGAATTCCC
CAATGTCAAG
CACTTCCGGA
ATCGGGAGCG
CGGCCGATGC
AAAGTGCCGA
TAAACATAAC
GATCTTTGTA
GAAACCATCG
GCGCAGCTAT
TTACCCGCAG
GACATATCCA
CGCCCTCCTA
CATCGAAGCT
GAAAGCACGA
GATTCTTCGC
CCTCCGAGAG
CTGCATCAGG
TCGGACACGC
TGTCGAACTT
TTCGATCAGA
AACTTCTCGA
CAGACGTCGC
GGTGAGTTCA
GGCTTTTTCA
TCCTAGGAGA
GATAGATTTG
TAGAGAGAGA
CTGGTGATTT
CAGCGTGTCC
TCTCCAAATG
AAATGAACTT
CCTTATATAG
AGGAAGGGTC
TTGCGAAGGA
TAGTGGGATT
GTGCGTCATC
CCTTACGTCA
GTGGAGATAT
CACATCAATC
CACTTGCTTT
GAAGACGTGG
TTGGAACGTC
TTCTTTTTCC
ACGATGCTCC
TCGTGGGTGG
GGGTCCATCT
TTGGGACCAC
TGTCGGCAGA
GGCATCTTGA
ACGATAGCCT
TTCCTTTATC
GCAATGATGG
CATTTGTAGG
TGCCACCTTC
CTTTTCTACT
GTCCTTTTGA
TGAAGTGACA
GATAGCTGGG
CAATGGAATC
CGAGGAGGTT
TCCCGATATT
ACCCTTTGTT
GAAAAGTCTC
AATAGCCCTT
TGGTCTTCTG
AGACTGTATC
TTTGATATTC
TTGGAGTAGA
CGAGAGTGTC
GTGCTCCACC
ATGTTCACAT
CAATCCACTT
GCTTTGAAGA
CGTGGTTGGA
ACGTCTTCTT
TTTCCACGAT
GCTCCTCGTG
GGTGGGGGTC
CATCTTTGGG
ACCACTGTCG
GCAGAGGCAT
CTTGAACGAT
AGCCTTTCCT
TTATCGCAAT
GATGGCATTT
GTAGGTGCCA
CCTTCCTTTT
CTACTGTCCT
TTTGATGAAG
TGACAGATAG
CTGGGCAATG
GAATCCGAGG
AGGTTTCCCG
ATATTACCCT
TTGTTGAAAA
GTCTCAATAG
CCCTTTGGTC
TTCTGAGACT
GTATCTT