名称: | pBUE411-AtU6-FPA-sgRNA1-sgRNA2-Ubi-Cas9 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | Basta |
荧光标记: | |
启动子: | AtU6 |
复制子: | pVS1 |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TAAACGCTCT
TTTCTCTTAG
GTTTACCCGC
CAATATATCC
TGTCAAACAC
TGATAGTTTA
AACTGAAGGC
GGGAAACGAC
AATCTGATCC
AAGCTCAAGC
TGCTCTAGCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGCTG
GCGAAAGGGG
GATGTGCTGC
AAGGCGATTA
AGTTGGGTAA
CGCCAGGGTT
TTCCCAGTCA
CGACGTTGTA
AAACGACGGC
CAGTGCCAAG
CTTCGACTTG
CCTTCCGCAC
AATACATCAT
TTCTTCTTAG
CTTTTTTTCT
TCTTCTTCGT
TCATACAGTT
TTTTTTTGTT
TATCAGCTTA
CATTTTCTTG
AACCGTAGCT
TTCGTTTTCT
TCTTTTTAAC
TTTCCATTCG
GAGTTTTTGT
ATCTTGTTTC
ATAGTTTGTC
CCAGGATTAG
AATGATTAGG
CATCGAACCT
TCAAGAATTT
GATTGAATAA
AACATCTTCA
TTCTTAAGAT
ATGAAGATAA
TCTTCAAAAG
GCCCCTGGGA
ATCTGAAAGA
AGAGAAGCAG
GCCCATTTAT
ATGGGAAAGA
ACAATAGTAT
TTCTTATATA
GGCCCATTTA
AGTTGAAAAC
AATCTTCAAA
AGTCCCACAT
CGCTTAGATA
AGAAAACGAA
GCTGAGTTTA
TATACAGCTA
GAGTCGAAGT
AGTGATTGCT
CTTCCTCCAG
GTCATCCTGT
TTTAGAGCTA
GAAATAGCAA
GTTAAAATAA
GGCTAGTCCG
TTATCAACTT
GAAAAAGTGG
CACCGAGTCG
GTGCTTTTTT
TTGCAAAATT
TTCCAGATCG
ATTTCTTCTT
CCTCTGTTCT
TCGGCCATCG
CTACCTTAGG
ACCGTTATAG
TTAATTACCC
TGTTATCCCT
ATTAATTAAG
AGCTCAGAAA
TCTCAAAATT
CCGGCAGAAC
AATTTTGAAT
CTCGATCCGT
AGAAACGAGA
CGGTCATTGT
TTTAGTTCCA
CCACGATTAT
ATTTGAAATT
TACGTGAGTG
TGAGTGAGAC
TTGCATAAGA
AAATAAAATC
TTTAGTTGGG
AAAAAATTCA
ATAATATAAA
TGGGCTTGAG
AAGGAAGCGA
GGGATAGGCC
TTTTTCTAAA
ATAGGCCCAT
TTAAGCTATT
AACAATCTTC
AAAAGTACCA
CAGCGCTTAG
GTAAAGAAAG
CAGCTGAGTT
TATATATGGT
TAGAGACGAA
GTAGTGATTG
TCCACAGAAC
CTTACTTGGC
GTTTTAGAGC
TAGAAATAGC
AAGTTAAAAT
AAGGCTAGTC
CGTTATCAAC
TTGAAAAAGT
GGCACCGAGT
CGGTGCTTTT
TTTCTAGACC
CAGCTTTCTT
GTACAAGCTT
GCATGCCTGC
AGTGCAGCGT
GACCCGGTCG
TGCCCCTCTC
TAGAGATAAT
GAGCATTGCA
TGTCTAAGTT
ATAAAAAATT
ACCACATATT
TTTTTTGTCA
CACTTGTTTG
AAGTGCAGTT
TATCTATCTT
TATACATATA
TTTAAACTTT
ACTCTACGAA
TAATATAATC
TATAGTACTA
CAATAATATC
AGTGTTTTAG
AGAATCATAT
AAATGAACAG
TTAGACATGG
TCTAAAGGAC
AATTGAGTAT
TTTGACAACA
GGACTCTACA
GTTTTATCTT
TTTAGTGTGC
ATGTGTTCTC
CTTTTTTTTT
GCAAATAGCT
TCACCTATAT
AATACTTCAT
CCATTTTATT
AGTACATCCA
TTTAGGGTTT
AGGGTTAATG
GTTTTTATAG
ACTAATTTTT
TTAGTACATC
TATTTTATTC
TATTTTAGCC
TCTAAATTAA
GAAAACTAAA
ACTCTATTTT
AGTTTTTTTA
TTTAATAATT
TAGATATAAA
ATAGAATAAA
ATAAAGTGAC
TAAAAATTAA
ACAAATACCC
TTTAAGAAAT
TAAAAAAACT
AAGGAAACAT
TTTTCTTGTT
TCGAGTAGAT
AATGCCAGCC
TGTTAAACGC
CGTCGACGAG
TCTAACGGAC
ACCAACCAGC
GAACCAGCAG
CGTCGCGTCG
GGCCAAGCGA
AGCAGACGGC
ACGGCATCTC
TGTCGCTGCC
TCTGGACCCC
TCTCGAGAGT
TCCGCTCCAC
CGTTGGACTT
GCTCCGCTGT
CGGCATCCAG
AAATTGCGTG
GCGGAGCGGC
AGACGTGAGC
CGGCACGGCA
GGCGGCCTCC
TCCTCCTCTC
ACGGCACGGC
AGCTACGGGG
GATTCCTTTC
CCACCGCTCC
TTCGCTTTCC
CTTCCTCGCC
CGCCGTAATA
AATAGACACC
CCCTCCACAC
CCTCTTTCCC
CAACCTCGTG
TTGTTCGGAG
CGCACACACA
CACAACCAGA
TCTCCCCCAA
ATCCACCCGT
CGGCACCTCC
GCTTCAAGGT
ACGCCGCTCG
TCCTCCCCCC
CCCCCCCTCT
CTACCTTCTC
TAGATCGGCG
TTCCGGTCCA
TGGTTAGGGC
CCGGTAGTTC
TACTTCTGTT
CATGTTTGTG
TTAGATCCGT
GTTTGTGTTA
GATCCGTGCT
GCTAGCGTTC
GTACACGGAT
GCGACCTGTA
CGTCAGACAC
GTTCTGATTG
CTAACTTGCC
AGTGTTTCTC
TTTGGGGAAT
CCTGGGATGG
CTCTAGCCGT
TCCGCAGACG
GGATCGATTT
CATGATTTTT
TTTGTTTCGT
TGCATAGGGT
TTGGTTTGCC
CTTTTCCTTT
ATTTCAATAT
ATGCCGTGCA
CTTGTTTGTC
GGGTCATCTT
TTCATGCTTT
TTTTTGTCTT
GGTTGTGATG
ATGTGGTCTG
GTTGGGCGGT
CGTTCTAGAT
CGGAGTAGAA
TTCTGTTTCA
AACTACCTGG
TGGATTTATT
AATTTTGGAT
CTGTATGTGT
GTGCCATACA
TATTCATAGT
TACGAATTGA
AGATGATGGA
TGGAAATATC
GATCTAGGAT
AGGTATACAT
GTTGATGCGG
GTTTTACTGA
TGCATATACA
GAGATGCTTT
TTGTTCGCTT
GGTTGTGATG
ATGTGGTGTG
GTTGGGCGGT
CGTTCATTCG
TTCTAGATCG
GAGTAGAATA
CTGTTTCAAA
CTACCTGGTG
TATTTATTAA
TTTTGGAACT
GTATGTGTGT
GTCATACATC
TTCATAGTTA
CGAGTTTAAG
ATGGATGGAA
ATATCGATCT
AGGATAGGTA
TACATGTTGA
TGTGGGTTTT
ACTGATGCAT
ATACATGATG
GCATATGCAG
CATCTATTCA
TATGCTCTAA
CCTTGAGTAC
CTATCTATTA
TAATAAACAA
GTATGTTTTA
TAATTATTTT
GATCTTGATA
TACTTGGATG
ATGGCATATG
CAGCAGCTAT
ATGTGGATTT
TTTTAGCCCT
GCCTTCATAC
GCTATTTATT
TGCTTGGTAC
TGTTTCTTTT
GTCGATGCTC
ACCCTGTTGT
TTGGTGTTAC
TTCTGCAGCC
CTAGGCCTAC
TAGATGGATT
ACAAGGACCA
CGACGGGGAT
TACAAGGACC
ACGACATTGA
TTACAAGGAT
GATGATGACA
AGATGGCTCC
GAAGAAGAAG
AGGAAGGTTG
GCATCCACGG
GGTGCCAGCT
GCTGACAAGA
AGTACTCGAT
CGGCCTCGAT
ATTGGGACTA
ACTCTGTTGG
CTGGGCCGTG
ATCACCGACG
AGTACAAGGT
GCCCTCAAAG
AAGTTCAAGG
TCCTGGGCAA
CACCGATCGG
CATTCCATCA
AGAAGAATCT
CATTGGCGCT
CTCCTGTTCG
ACAGCGGCGA
GACGGCTGAG
GCTACGCGGC
TCAAGCGCAC
CGCCCGCAGG
CGGTACACGC
GCAGGAAGAA
TCGCATCTGC
TACCTGCAGG
AGATTTTCTC
CAACGAGATG
GCGAAGGTTG
ACGATTCTTT
CTTCCACAGG
CTGGAGGAGT
CATTCCTCGT
GGAGGAGGAT
AAGAAGCACG
AGCGGCATCC
AATCTTCGGC
AACATTGTCG
ACGAGGTTGC
CTACCACGAG
AAGTACCCTA
CGATCTACCA
TCTGCGGAAG
AAGCTCGTGG
ACTCCACAGA
TAAGGCGGAC
CTCCGCCTGA
TCTACCTCGC
TCTGGCCCAC
ATGATTAAGT
TCAGGGGCCA
TTTCCTGATC
GAGGGGGATC
TCAACCCGGA
CAATAGCGAT
GTTGACAAGC
TGTTCATCCA
GCTCGTGCAG
ACGTACAACC
AGCTCTTCGA
GGAGAACCCC
ATTAATGCGT
CAGGCGTCGA
CGCGAAGGCT
ATCCTGTCCG
CTAGGCTCTC
GAAGTCTCGG
CGCCTCGAGA
ACCTGATCGC
CCAGCTGCCG
GGCGAGAAGA
AGAACGGCCT
GTTCGGGAAT
CTCATTGCGC
TCAGCCTGGG
GCTCACGCCC
AACTTCAAGT
CGAATTTCGA
TCTCGCTGAG
GACGCCAAGC
TGCAGCTCTC
CAAGGACACA
TACGACGATG
ACCTGGATAA
CCTCCTGGCC
CAGATCGGCG
ATCAGTACGC
GGACCTGTTC
CTCGCTGCCA
AGAATCTGTC
GGACGCCATC
CTCCTGTCTG
ATATTCTCAG
GGTGAACACC
GAGATTACGA
AGGCTCCGCT
CTCAGCCTCC
ATGATCAAGC
GCTACGACGA
GCACCATCAG
GATCTGACCC
TCCTGAAGGC
GCTGGTCAGG
CAGCAGCTCC
CCGAGAAGTA
CAAGGAGATC
TTCTTCGATC
AGTCGAAGAA
CGGCTACGCT
GGGTACATTG
ACGGCGGGGC
CTCTCAGGAG
GAGTTCTACA
AGTTCATCAA
GCCGATTCTG
GAGAAGATGG
ACGGCACGGA
GGAGCTGCTG
GTGAAGCTCA
ATCGCGAGGA
CCTCCTGAGG
AAGCAGCGGA
CATTCGATAA
CGGCAGCATC
CCACACCAGA
TTCATCTCGG
GGAGCTGCAC
GCTATCCTGA
GGAGGCAGGA
GGACTTCTAC
CCTTTCCTCA
AGGATAACCG
CGAGAAGATC
GAGAAGATTC
TGACTTTCAG
GATCCCGTAC
TACGTCGGCC
CACTCGCTAG
GGGCAACTCC
CGCTTCGCTT
GGATGACCCG
CAAGTCAGAG
GAGACGATCA
CGCCGTGGAA
CTTCGAGGAG
GTGGTCGACA
AGGGCGCTAG
CGCTCAGTCG
TTCATCGAGA
GGATGACGAA
TTTCGACAAG
AACCTGCCAA
ATGAGAAGGT
GCTCCCTAAG
CACTCGCTCC
TGTACGAGTA
CTTCACAGTC
TACAACGAGC
TGACTAAGGT
GAAGTATGTG
ACCGAGGGCA
TGAGGAAGCC
GGCTTTCCTG
TCTGGGGAGC
AGAAGAAGGC
CATCGTGGAC
CTCCTGTTCA
AGACCAACCG
GAAGGTCACG
GTTAAGCAGC
TCAAGGAGGA
CTACTTCAAG
AAGATTGAGT
GCTTCGATTC
GGTCGAGATC
TCTGGCGTTG
AGGACCGCTT
CAACGCCTCC
CTGGGGACCT
ACCACGATCT
CCTGAAGATC
ATTAAGGATA
AGGACTTCCT
GGACAACGAG
GAGAATGAGG
ATATCCTCGA
GGACATTGTG
CTGACACTCA
CTCTGTTCGA
GGACCGGGAG
ATGATCGAGG
AGCGCCTGAA
GACTTACGCC
CATCTCTTCG
ATGACAAGGT
CATGAAGCAG
CTCAAGAGGA
GGAGGTACAC
CGGCTGGGGG
AGGCTGAGCA
GGAAGCTCAT
CAACGGCATT
CGGGACAAGC
AGTCCGGGAA
GACGATCCTC
GACTTCCTGA
AGAGCGATGG
CTTCGCGAAC
CGCAATTTCA
TGCAGCTGAT
TCACGATGAC
AGCCTCACAT
TCAAGGAGGA
TATCCAGAAG
GCTCAGGTGA
GCGGCCAGGG
GGACTCGCTG
CACGAGCATA
TCGCGAACCT
CGCTGGCTCG
CCAGCTATCA
AGAAGGGGAT
TCTGCAGACC
GTGAAGGTTG
TGGACGAGCT
GGTGAAGGTC
ATGGGCAGGC
ACAAGCCTGA
GAACATCGTC
ATTGAGATGG
CCCGGGAGAA
TCAGACCACG
CAGAAGGGCC
AGAAGAACTC
ACGCGAGAGG
ATGAAGAGGA
TCGAGGAGGG
CATTAAGGAG
CTGGGGTCCC
AGATCCTCAA
GGAGCACCCG
GTGGAGAACA
CGCAGCTGCA
GAATGAGAAG
CTCTACCTGT
ACTACCTCCA
GAATGGCCGC
GATATGTATG
TGGACCAGGA
GCTGGATATT
AACAGGCTCA
GCGATTACGA
CGTCGATCAT
ATCGTTCCAC
AGTCATTCCT
GAAGGATGAC
TCCATTGACA
ACAAGGTCCT
CACCAGGTCG
GACAAGAACC
GGGGCAAGTC
TGATAATGTT
CCTTCAGAGG
AGGTCGTTAA
GAAGATGAAG
AACTACTGGC
GCCAGCTCCT
GAATGCCAAG
CTGATCACGC
AGCGGAAGTT
CGATAACCTC
ACAAAGGCTG
AGAGGGGCGG
GCTCTCTGAG
CTGGACAAGG
CGGGCTTCAT
CAAGAGGCAG
CTGGTCGAGA
CACGGCAGAT
CACTAAGCAC
GTTGCGCAGA
TTCTCGACTC
ACGGATGAAC
ACTAAGTACG
ATGAGAATGA
CAAGCTGATC
CGCGAGGTGA
AGGTCATCAC
CCTGAAGTCA
AAGCTCGTCT
CCGACTTCAG
GAAGGATTTC
CAGTTCTACA
AGGTTCGGGA
GATCAACAAT
TACCACCATG
CCCATGACGC
GTACCTGAAC
GCGGTGGTCG
GCACAGCTCT
GATCAAGAAG
TACCCAAAGC
TCGAGAGCGA
GTTCGTGTAC
GGGGACTACA
AGGTTTACGA
TGTGAGGAAG
ATGATCGCCA
AGTCGGAGCA
GGAGATTGGC
AAGGCTACCG
CCAAGTACTT
CTTCTACTCT
AACATTATGA
ATTTCTTCAA
GACAGAGATC
ACTCTGGCCA
ATGGCGAGAT
CCGGAAGCGC
CCCCTCATCG
AGACGAACGG
CGAGACGGGG
GAGATCGTGT
GGGACAAGGG
CAGGGATTTC
GCGACCGTCA
GGAAGGTTCT
CTCCATGCCA
CAAGTGAATA
TCGTCAAGAA
GACAGAGGTC
CAGACTGGCG
GGTTCTCTAA
GGAGTCAATT
CTGCCTAAGC
GGAACAGCGA
CAAGCTCATC
GCCCGCAAGA
AGGACTGGGA
TCCGAAGAAG
TACGGCGGGT
TCGACAGCCC
CACTGTGGCC
TACTCGGTCC
TGGTTGTGGC
GAAGGTTGAG
AAGGGCAAGT
CCAAGAAGCT
CAAGAGCGTG
AAGGAGCTGC
TGGGGATCAC
GATTATGGAG
CGCTCCAGCT
TCGAGAAGAA
CCCGATCGAT
TTCCTGGAGG
CGAAGGGCTA
CAAGGAGGTG
AAGAAGGACC
TGATCATTAA
GCTCCCCAAG
TACTCACTCT
TCGAGCTGGA
GAACGGCAGG
AAGCGGATGC
TGGCTTCCGC
TGGCGAGCTG
CAGAAGGGGA
ACGAGCTGGC
TCTGCCGTCC
AAGTATGTGA
ACTTCCTCTA
CCTGGCCTCC
CACTACGAGA
AGCTCAAGGG
CAGCCCCGAG
GACAACGAGC
AGAAGCAGCT
GTTCGTCGAG
CAGCACAAGC
ATTACCTCGA
CGAGATCATT
GAGCAGATTT
CCGAGTTCTC
CAAGCGCGTG
ATCCTGGCCG
ACGCGAATCT
GGATAAGGTC
CTCTCCGCGT
ACAACAAGCA
CCGCGACAAG
CCAATCAGGG
AGCAGGCTGA
GAATATCATT
CATCTCTTCA
CCCTGACGAA
CCTCGGCGCC
CCTGCTGCTT
TCAAGTACTT
CGACACAACT
ATCGATCGCA
AGAGGTACAC
AAGCACTAAG
GAGGTCCTGG
ACGCGACCCT
CATCCACCAG
TCGATTACCG
GCCTCTACGA
GACGCGCATC
GACCTGTCTC
AGCTCGGGGG
CGACAAGCGG
CCAGCGGCGA
CGAAGAAGGC
GGGGCAGGCG
AAGAAGAAGA
AGTGAGCTCA
GAGCTTTCGT
TCGTATCATC
GGTTTCGACA
ACGTTCGTCA
AGTTCAATGC
ATCAGTTTCA
TTGCGCACAC
ACCAGAATCC
TACTGAGTTT
GAGTATTATG
GCATTGGGAA
AACTGTTTTT
CTTGTACCAT
TTGTTGTGCT
TGTAATTTAC
TGTGTTTTTT
ATTCGGTTTT
CGCTATCGAA
CTGTGAAATG
GAAATGGATG
GAGAAGAGTT
AATGAATGAT
ATGGTCCTTT
TGTTCATTCT
CAAATTAATA
TTATTTGTTT
TTTCTCTTAT
TTGTTGTGTG
TTGAATTTGA
AATTATAAGA
GATATGCAAA
CATTTTGTTT
TGAGTAAAAA
TGTGTCAAAT
CGTGGCCTCT
AATGACCGAA
GTTAATATGA
GGAGTAAAAC
ACTTGTAGTT
GTACCATTAT
GCTTATTCAC
TAGGCAACAA
ATATATTTTC
AGACCTAGAA
AAGCTGCAAA
TGTTACTGAA
TACAAGTATG
TCCTCTTGTG
TTTTAGACAT
TTATGAACTT
TCCTTTATGT
AATTTTCCAG
AATCCTTGTC
AGATTCTAAT
CATTGCTTTA
TAATTATAGT
TATACTCATG
GATTTGTAGT
TGAGTATGAA
AATATTTTTT
AATGCATTTT
ATGACTTGCC
AATTGATTGA
CAACGAATTC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGCTAGA
GCAGCTTGCC
AACATGGTGG
AGCACGACAC
TCTCGTCTAC
TCCAAGAATA
TCAAAGATAC
AGTCTCAGAA
GACCAAAGGG
CTATTGAGAC
TTTTCAACAA
AGGGTAATAT
CGGGAAACCT
CCTCGGATTC
CATTGCCCAG
CTATCTGTCA
CTTCATCAAA
AGGACAGTAG
AAAAGGAAGG
TGGCACCTAC
AAATGCCATC
ATTGCGATAA
AGGAAAGGCT
ATCGTTCAAG
ATGCCTCTGC
CGACAGTGGT
CCCAAAGATG
GACCCCCACC
CACGAGGAGC
ATCGTGGAAA
AAGAAGACGT
TCCAACCACG
TCTTCAAAGC
AAGTGGATTG
ATGTGATAAC
ATGGTGGAGC
ACGACACTCT
CGTCTACTCC
AAGAATATCA
AAGATACAGT
CTCAGAAGAC
CAAAGGGCTA
TTGAGACTTT
TCAACAAAGG
GTAATATCGG
GAAACCTCCT
CGGATTCCAT
TGCCCAGCTA
TCTGTCACTT
CATCAAAAGG
ACAGTAGAAA
AGGAAGGTGG
CACCTACAAA
TGCCATCATT
GCGATAAAGG
AAAGGCTATC
GTTCAAGATG
CCTCTGCCGA
CAGTGGTCCC
AAAGATGGAC
CCCCACCCAC
GAGGAGCATC
GTGGAAAAAG
AAGACGTTCC
AACCACGTCT
TCAAAGCAAG
TGGATTGATG
TGATATCTCC
ACTGACGTAA
GGGATGACGC
ACAATCCCAC
TATCCTTCGC
AAGACCTTCC
TCTATATAAG
GAAGTTCATT
TCATTTGGAG
AGGACACGCT
GAAATCACCA
GTCTCTCTCT
ACAAATCTAT
CTCTCTCGAG
TCTACCATGA
GCCCAGAACG
ACGCCCGGCC
GACATCCGCC
GTGCCACCGA
GGCGGACATG
CCGGCGGTCT
GCACCATCGT
CAACCACTAC
ATCGAGACAA
GCACGGTCAA
CTTCCGTACC
GAGCCGCAGG
AACCGCAGGA
GTGGACGGAC
GACCTCGTCC
GTCTGCGGGA
GCGCTATCCC
TGGCTCGTCG
CCGAGGTGGA
CGGCGAGGTC
GCCGGCATCG
CCTACGCGGG
CCCCTGGAAG
GCACGCAACG
CCTACGACTG
GACGGCCGAG
TCGACCGTGT
ACGTCTCCCC
CCGCCACCAG
CGGACGGGAC
TGGGCTCCAC
GCTCTACACC
CACCTGCTGA
AGTCCCTGGA
GGCACAGGGC
TTCAAGAGCG
TGGTCGCTGT
CATCGGGCTG
CCCAACGACC
CGAGCGTGCG
CATGCACGAG
GCGCTCGGAT
ATGCCCCCCG
CGGCATGCTG
CGGGCGGCCG
GCTTCAAGCA
CGGGAACTGG
CATGACGTGG
GTTTCTGGCA
GCTGGACTTC
AGCCTGCCGG
TACCGCCCCG
TCCGGTCCTG
CCCGTCACCG
AGATTTGACT
CGAGTTTCTC
CATAATAATG
TGTGAGTAGT
TCCCAGATAA
GGGAATTAGG
GTTCCTATAG
GGTTTCGCTC
ATGTGTTGAG
CATATAAGAA
ACCCTTAGTA
TGTATTTGTA
TTTGTAAAAT
ACTTCTATCA
ATAAAATTTC
TAATTCCTAA
AACCAAAATC
CAGTACTAAA
ATCCAGATCC
CCCGAATTAA
TTCGGCGTTA
ATTCAGTACA
TTAAAAACGT
CCGCAATGTG
TTATTAAGTT
GTCTAAGCGT
CAATTTGTTT
ACACCACAAT
ATATCCTGCC
ACCAGCCAGC
CAACAGCTCC
CCGACCGGCA
GCTCGGCACA
AAATCACCAC
TCGATACAGG
CAGCCCATCA
GTCCGGGACG
GCGTCAGCGG
GAGAGCCGTT
GTAAGGCGGC
AGACTTTGCT
CATGTTACCG
ATGCTATTCG
GAAGAACGGC
AACTAAGCTG
CCGGGTTTGA
AACACGGATG
ATCTCGCGGA
GGGTAGCATG
TTGATTGTAA
CGATGACAGA
GCGTTGCTGC
CTGTGATCAC
CGCGGTTTCA
AAATCGGCTC
CGTCGATACT
ATGTTATACG
CCAACTTTGA
AAACAACTTT
GAAAAAGCTG
TTTTCTGGTA
TTTAAGGTTT
TAGAATGCAA
GGAACAGTGA
ATTGGAGTTC
GTCTTGTTAT
AATTAGCTTC
TTGGGGTATC
TTTAAATACT
GTAGAAAAGA
GGAAGGAAAT
AATAAATGGC
TAAAATGAGA
ATATCACCGG
AATTGAAAAA
ACTGATCGAA
AAATACCGCT
GCGTAAAAGA
TACGGAAGGA
ATGTCTCCTG
CTAAGGTATA
TAAGCTGGTG
GGAGAAAATG
AAAACCTATA
TTTAAAAATG
ACGGACAGCC
GGTATAAAGG
GACCACCTAT
GATGTGGAAC
GGGAAAAGGA
CATGATGCTA
TGGCTGGAAG
GAAAGCTGCC
TGTTCCAAAG
GTCCTGCACT
TTGAACGGCA
TGATGGCTGG
AGCAATCTGC
TCATGAGTGA
GGCCGATGGC
GTCCTTTGCT
CGGAAGAGTA
TGAAGATGAA
CAAAGCCCTG
AAAAGATTAT
CGAGCTGTAT
GCGGAGTGCA
TCAGGCTCTT
TCACTCCATC
GACATATCGG
ATTGTCCCTA
TACGAATAGC
TTAGACAGCC
GCTTAGCCGA
ATTGGATTAC
TTACTGAATA
ACGATCTGGC
CGATGTGGAT
TGCGAAAACT
GGGAAGAAGA
CACTCCATTT
AAAGATCCGC
GCGAGCTGTA
TGATTTTTTA
AAGACGGAAA
AGCCCGAAGA
GGAACTTGTC
TTTTCCCACG
GCGACCTGGG
AGACAGCAAC
ATCTTTGTGA
AAGATGGCAA
AGTAAGTGGC
TTTATTGATC
TTGGGAGAAG
CGGCAGGGCG
GACAAGTGGT
ATGACATTGC
CTTCTGCGTC
CGGTCGATCA
GGGAGGATAT
CGGGGAAGAA
CAGTATGTCG
AGCTATTTTT
TGACTTACTG
GGGATCAAGC
CTGATTGGGA
GAAAATAAAA
TATTATATTT
TACTGGATGA
ATTGTTTTAG
TACCTAGAAT
GCATGACCAA
AATCCCTTAA
CGTGAGTTTT
CGTTCCACTG
AGCGTCAGAC
CCCGTAGAAA
AGATCAAAGG
ATCTTCTTGA
GATCCTTTTT
TTCTGCGCGT
AATCTGCTGC
TTGCAAACAA
AAAAACCACC
GCTACCAGCG
GTGGTTTGTT
TGCCGGATCA
AGAGCTACCA
ACTCTTTTTC
CGAAGGTAAC
TGGCTTCAGC
AGAGCGCAGA
TACCAAATAC
TGTCCTTCTA
GTGTAGCCGT
AGTTAGGCCA
CCACTTCAAG
AACTCTGTAG
CACCGCCTAC
ATACCTCGCT
CTGCTAATCC
TGTTACCAGT
GGCTGCTGCC
AGTGGCGATA
AGTCGTGTCT
TACCGGGTTG
GACTCAAGAC
GATAGTTACC
GGATAAGGCG
CAGCGGTCGG
GCTGAACGGG
GGGTTCGTGC
ACACAGCCCA
GCTTGGAGCG
AACGACCTAC
ACCGAACTGA
GATACCTACA
GCGTGAGCTA
TGAGAAAGCG
CCACGCTTCC
CGAAGGGAGA
AAGGCGGACA
GGTATCCGGT
AAGCGGCAGG
GTCGGAACAG
GAGAGCGCAC
GAGGGAGCTT
CCAGGGGGAA
ACGCCTGGTA
TCTTTATAGT
CCTGTCGGGT
TTCGCCACCT
CTGACTTGAG
CGTCGATTTT
TGTGATGCTC
GTCAGGGGGG
CGGAGCCTAT
GGAAAAACGC
CAGCAACGCG
GCCTTTTTAC
GGTTCCTGGC
CTTTTGCTGG
CCTTTTGCTC
ACATGTTCTT
TCCTGCGTTA
TCCCCTGATT
CTGTGGATAA
CCGTATTACC
GCCTTTGAGT
GAGCTGATAC
CGCTCGCCGC
AGCCGAACGA
CCGAGCGCAG
CGAGTCAGTG
AGCGAGGAAG
CGGAAGAGCG
CCTGATGCGG
TATTTTCTCC
TTACGCATCT
GTGCGGTATT
TCACACCGCA
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATACACTCC
GCTATCGCTA
CGTGACTGGG
TCATGGCTGC
GCCCCGACAC
CCGCCAACAC
CCGCTGACGC
GCCCTGACGG
GCTTGTCTGC
TCCCGGCATC
CGCTTACAGA
CAAGCTGTGA
CCGTCTCCGG
GAGCTGCATG
TGTCAGAGGT
TTTCACCGTC
ATCACCGAAA
CGCGCGAGGC
AGGGTGCCTT
GATGTGGGCG
CCGGCGGTCG
AGTGGCGACG
GCGCGGCTTG
TCCGCGCCCT
GGTAGATTGC
CTGGCCGTAG
GCCAGCCATT
TTTGAGCGGC
CAGCGGCCGC
GATAGGCCGA
CGCGAAGCGG
CGGGGCGTAG
GGAGCGCAGC
GACCGAAGGG
TAGGCGCTTT
TTGCAGCTCT
TCGGCTGTGC
GCTGGCCAGA
CAGTTATGCA
CAGGCCAGGC
GGGTTTTAAG
AGTTTTAATA
AGTTTTAAAG
AGTTTTAGGC
GGAAAAATCG
CCTTTTTTCT
CTTTTATATC
AGTCACTTAC
ATGTGTGACC
GGTTCCCAAT
GTACGGCTTT
GGGTTCCCAA
TGTACGGGTT
CCGGTTCCCA
ATGTACGGCT
TTGGGTTCCC
AATGTACGTG
CTATCCACAG
GAAACAGACC
TTTTCGACCT
TTTTCCCCTG
CTAGGGCAAT
TTGCCCTAGC
ATCTGCTCCG
TACATTAGGA
ACCGGCGGAT
GCTTCGCCCT
CGATCAGGTT
GCGGTAGCGC
ATGACTAGGA
TCGGGCCAGC
CTGCCCCGCC
TCCTCCTTCA
AATCGTACTC
CGGCAGGTCA
TTTGACCCGA
TCAGCTTGCG
CACGGTGAAA
CAGAACTTCT
TGAACTCTCC
GGCGCTGCCA
CTGCGTTCGT
AGATCGTCTT
GAACAACCAT
CTGGCTTCTG
CCTTGCCTGC
GGCGCGGCGT
GCCAGGCGGT
AGAGAAAACG
GCCGATGCCG
GGATCGATCA
AAAAGTAATC
GGGGTGAACC
GTCAGCACGT
CCGGGTTCTT
GCCTTCTGTG
ATCTCGCGGT
ACATCCAATC
AGCTAGCTCG
ATCTCGATGT
ACTCCGGCCG
CCCGGTTTCG
CTCTTTACGA
TCTTGTAGCG
GCTAATCAAG
GCTTCACCCT
CGGATACCGT
CACCAGGCGG
CCGTTCTTGG
CCTTCTTCGT
ACGCTGCATG
GCAACGTGCG
TGGTGTTTAA
CCGAATGCAG
GTTTCTACCA
GGTCGTCTTT
CTGCTTTCCG
CCATCGGCTC
GCCGGCAGAA
CTTGAGTACG
TCCGCAACGT
GTGGACGGAA
CACGCGGCCG
GGCTTGTCTC
CCTTCCCTTC
CCGGTATCGG
TTCATGGATT
CGGTTAGATG
GGAAACCGCC
ATCAGTACCA
GGTCGTAATC
CCACACACTG
GCCATGCCGG
CCGGCCCTGC
GGAAACCTCT
ACGTGCCCGT
CTGGAAGCTC
GTAGCGGATC
ACCTCGCCAG
CTCGTCGGTC
ACGCTTCGAC
AGACGGAAAA
CGGCCACGTC
CATGATGCTG
CGACTATCGC
GGGTGCCCAC
GTCATAGAGC
ATCGGAACGA
AAAAATCTGG
TTGCTCGTCG
CCCTTGGGCG
GCTTCCTAAT
CGACGGCGCA
CCGGCTGCCG
GCGGTTGCCG
GGATTCTTTG
CGGATTCGAT
CAGCGGCCGC
TTGCCACGAT
TCACCGGGGC
GTGCTTCTGC
CTCGATGCGT
TGCCGCTGGG
CGGCCTGCGC
GGCCTTCAAC
TTCTCCACCA
GGTCATCACC
CAGCGCCGCG
CCGATTTGTA
CCGGGCCGGA
TGGTTTGCGA
CCGCTCACGC
CGATTCCTCG
GGCTTGGGGG
TTCCAGTGCC
ATTGCAGGGC
CGGCAGACAA
CCCAGCCGCT
TACGCCTGGC
CAACCGCCCG
TTCCTCCACA
CATGGGGCAT
TCCACGGCGT
CGGTGCCTGG
TTGTTCTTGA
TTTTCCATGC
CGCCTCCTTT
AGCCGCTAAA
ATTCATCTAC
TCATTTATTC
ATTTGCTCAT
TTACTCTGGT
AGCTGCGCGA
TGTATTCAGA
TAGCAGCTCG
GTAATGGTCT
TGCCTTGGCG
TACCGCGTAC
ATCTTCAGCT
TGGTGTGATC
CTCCGCCGGC
AACTGAAAGT
TGACCCGCTT
CATGGCTGGC
GTGTCTGCCA
GGCTGGCCAA
CGTTGCAGCC
TTGCTGCTGC
GTGCGCTCGG
ACGGCCGGCA
CTTAGCGTGT
TTGTGCTTTT
GCTCATTTTC
TCTTTACCTC
ATTAACTCAA
ATGAGTTTTG
ATTTAATTTC
AGCGGCCAGC
GCCTGGACCT
CGCGGGCAGC
GTCGCCCTCG
GGTTCTGATT
CAAGAACGGT
TGTGCCGGCG
GCGGCAGTGC
CTGGGTAGCT
CACGCGCTGC
GTGATACGGG
ACTCAAGAAT
GGGCAGCTCG
TACCCGGCCA
GCGCCTCGGC
AACCTCACCG
CCGATGCGCG
TGCCTTTGAT
CGCCCGCGAC
ACGACAAAGG
CCGCTTGTAG
CCTTCCATCC
GTGACCTCAA
TGCGCTGCTT
AACCAGCTCC
ACCAGGTCGG
CGGTGGCCCA
TATGTCGTAA
GGGCTTGGCT
GCACCGGAAT
CAGCACGAAG
TCGGCTGCCT
TGATCGCGGA
CACAGCCAAG
TCCGCCGCCT
GGGGCGCTCC
GTCGATCACT
ACGAAGTCGC
GCCGGCCGAT
GGCCTTCACG
TCGCGGTCAA
TCGTCGGGCG
GTCGATGCCG
ACAACGGTTA
GCGGTTGATC
TTCCCGCACG
GCCGCCCAAT
CGCGGGCACT
GCCCTGGGGA
TCGGAATCGA
CTAACAGAAC
ATCGGCCCCG
GCGAGTTGCA
GGGCGCGGGC
TAGATGGGTT
GCGATGGTCG
TCTTGCCTGA
CCCGCCTTTC
TGGTTAAGTA
CAGCGATAAC
CTTCATGCGT
TCCCCTTGCG
TATTTGTTTA
TTTACTCATC
GCATCATATA
CGCAGCGACC
GCATGACGCA
AGCTGTTTTA
CTCAAATACA
CATCACCTTT
TTAGACGGCG
GCGCTCGGTT
TCTTCAGCGG
CCAAGCTGGC
CGGCCAGGCC
GCCAGCTTGG
CATCAGACAA
ACCGGCCAGG
ATTTCATGCA
GCCGCACGGT
TGAGACGTGC
GCGGGCGGCT
CGAACACGTA
CCCGGCCGCG
ATCATCTCCG
CCTCGATCTC
TTCGGTAATG
AAAAACGGTT
CGTCCTGGCC
GTCCTGGTGC
GGTTTCATGC
TTGTTCCTCT
TGGCGTTCAT
TCTCGGCGGC
CGCCAGGGCG
TCGGCCTCGG
TCAATGCGTC
CTCACGGAAG
GCACCGCGCC
GCCTGGCCTC
GGTGGGCGTC
ACTTCCTCGC
TGCGCTCAAG
TGCGCGGTAC
AGGGTCGAGC
GATGCACGCC
AAGCAGTGCA
GCCGCCTCTT
TCACGGTGCG
GCCTTCCTGG
TCGATCAGCT
CGCGGGCGTG
CGCGATCTGT
GCCGGGGTGA
GGGTAGGGCG
GGGGCCAAAC
TTCACGCCTC
GGGCCTTGGC
GGCCTCGCGC
CCGCTCCGGG
TGCGGTCGAT
GATTAGGGAA
CGCTCGAACT
CGGCAATGCC
GGCGAACACG
GTCAACACCA
TGCGGCCGGC
CGGCGTGGTG
GTGTCGGCCC
ACGGCTCTGC
CAGGCTACGC
AGGCCCGCGC
CGGCCTCCTG
GATGCGCTCG
GCAATGTCCA
GTAGGTCGCG
GGTGCTGCGG
GCCAGGCGGT
CTAGCCTGGT
CACTGTCACA
ACGTCGCCAG
GGCGTAGGTG
GTCAAGCATC
CTGGCCAGCT
CCGGGCGGTC
GCGCCTGGTG
CCGGTGATCT
TCTCGGAAAA
CAGCTTGGTG
CAGCCGGCCG
CGTGCAGTTC
GGCCCGTTGG
TTGGTCAAGT
CCTGGTCGTC
GGTGCTGACG
CGGGCATAGC
CCAGCAGGCC
AGCGGCGGCG
CTCTTGTTCA
TGGCGTAATG
TCTCCGGTTC
TAGTCGCAAG
TATTCTACTT
TATGCGACTA
AAACACGCGA
CAAGAAAACG
CCAGGAAAAG
GGCAGGGCGG
CAGCCTGTCG
CGTAACTTAG
GACTTGTGCG
ACATGTCGTT
TTCAGAAGAC
GGCTGCACTG
AACGTCAGAA
GCCGACTGCA
CTATAGCAGC
GGAGGGGTTG
GATCAAAGTA
CTTTGATCCC
GAGGGGAACC
CTGTGGTTGG
CATGCACATA
CAAATGGACG
AACGGATAAA
CCTTTTCACG
CCCTTTTAAA
TATCCGTTAT
TCTAA