名称: | pHSN501-AtU6-FPA-sgRNA1-sgRNA2-35S-Cas9 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan,Spe |
筛选标记: | Hyg |
荧光标记: | |
启动子: | AtU6-26 |
复制子: | pSa |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTTTACCCGC
CAATATATCC
TGTCAAACAC
TGATAGTTTA
AACTGAAGGC
GGGAAACGAC
AATCTGATCC
AAGCTCAAGC
TAAGCTCACG
TGACGGAATT
AAGCTTCGAC
TTGCCTTCCG
CACAATACAT
CATTTCTTCT
TAGCTTTTTT
TCTTCTTCTT
CGTTCATACA
GTTTTTTTTT
GTTTATCAGC
TTACATTTTC
TTGAACCGTA
GCTTTCGTTT
TCTTCTTTTT
AACTTTCCAT
TCGGAGTTTT
TGTATCTTGT
TTCATAGTTT
GTCCCAGGAT
TAGAATGATT
AGGCATCGAA
CCTTCAAGAA
TTTGATTGAA
TAAAACATCT
TCATTCTTAA
GATATGAAGA
TAATCTTCAA
AAGGCCCCTG
GGAATCTGAA
AGAAGAGAAG
CAGGCCCATT
TATATGGGAA
AGAACAATAG
TATTTCTTAT
ATAGGCCCAT
TTAAGTTGAA
AACAATCTTC
AAAAGTCCCA
CATCGCTTAG
ATAAGAAAAC
GAAGCTGAGT
TTATATACAG
CTAGAGTCGA
AGTAGTGATT
GTTACAGGCT
AAGAGCATGA
AGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTATCAA
CTTGAAAAAG
TGGCACCGAG
TCGGTGCTTT
TTTTTGCAAA
ATTTTCCAGA
TCGATTTCTT
CTTCCTCTGT
TCTTCGGCCA
TCGCTACCTT
AGGACCGTTA
TAGTTAATTA
CCCTGTTATC
CCTATTAATT
AAGAGCTCAG
AAATCTCAAA
ATTCCGGCAG
AACAATTTTG
AATCTCGATC
CGTAGAAACG
AGACGGTCAT
TGTTTTAGTT
CCACCACGAT
TATATTTGAA
ATTTACGTGA
GTGTGAGTGA
GACTTGCATA
AGAAAATAAA
ATCTTTAGTT
GGGAAAAAAT
TCAATAATAT
AAATGGGCTT
GAGAAGGAAG
CGAGGGATAG
GCCTTTTTCT
AAAATAGGCC
CATTTAAGCT
ATTAACAATC
TTCAAAAGTA
CCACAGCGCT
TAGGTAAAGA
AAGCAGCTGA
GTTTATATAT
GGTTAGAGAC
GAAGTAGTGA
TTGCGCAAAA
AGCTACCACC
CATGTTTTAG
AGCTAGAAAT
AGCAAGTTAA
AATAAGGCTA
GTCCGTTATC
AACTTGAAAA
AGTGGCACCG
AGTCGGTGCT
TTTTTTTGCA
AAATTTTCCA
GATCGATTTC
TTCTTCCTCT
GTTCTTCGGC
GTTCAATTTC
TGGGGTTTTC
TCTTCGTTTT
CTGTAACTGA
AACCTAAAAT
TTGACCTAAA
AAAAATCTCA
AATAATATGA
TTCAGTGGTT
TTGTACTTTT
CAGTTAGTTG
AGTTTTGCAG
TTCCGATGAG
ATAAACCAAT
AAGCTTGCAT
GCCTGCAGGT
CAACATGGTG
GAGCACGACA
CACTTGTCTA
CTCCAAAAAT
ATCAAAGATA
CAGTCTCAGA
AGACCAAAGG
GCAATTGAGA
CTTTTCAACA
AAGGGTAATA
TCCGGAAACC
TCCTCGGATT
CCATTGCCCA
GCTATCTGTC
ACTTTATTGT
GAAGATAGTG
GAAAAGGAAG
GTGGCTCCTA
CAAATGCCAT
CATTGCGATA
AAGGAAAGGC
CATCGTTGAA
GATGCCTCTG
CCGACAGTGG
TCCCAAAGAT
GGACCCCCAC
CCACGAGGAG
CATCGTGGAA
AAAGAAGACG
TTCCAACCAC
GTCTTCAAAG
CAAGTGGATT
GATGTGATAA
CATGGTGGAG
CACGACACAC
TTGTCTACTC
CAAAAATATC
AAAGATACAG
TCTCAGAAGA
CCAAAGGGCA
ATTGAGACTT
TTCAACAAAG
GGTAATATCC
GGAAACCTCC
TCGGATTCCA
TTGCCCAGCT
ATCTGTCACT
TTATTGTGAA
GATAGTGGAA
AAGGAAGGTG
GCTCCTACAA
ATGCCATCAT
TGCGATAAAG
GAAAGGCCAT
CGTTGAAGAT
GCCTCTGCCG
ACAGTGGTCC
CAAAGATGGA
CCCCCACCCA
CGAGGAGCAT
CGTGGAAAAA
GAAGACGTTC
CAACCACGTC
TTCAAAGCAA
GTGGATTGAT
GTGATATCTC
CACTGACGTA
AGGGATGACG
CACAATCCCA
CTATCCTTCG
CAAGACCCTT
CCTCTATATA
AGGAAGTTCA
TTTCATTTGG
AGAGGACCTC
GACCTCAACA
CAACATATAC
AAAACAAACG
AATCTCAAGC
AATCAAGCAT
TCTACTTCTA
TTGCAGCAAT
TTAAATCATT
TCTTTTAAAG
CAAAAGCAAT
TTTCTGAAAA
TTTTCACCAT
TTACGAACGA
TACTCGAGGG
GGATCCCCAA
TACTTGTATG
GCCGCGGCCG
CTCTAGATGG
ATTACAAGGA
CCACGACGGG
GATTACAAGG
ACCACGACAT
TGATTACAAG
GATGATGATG
ACAAGATGGC
TCCGAAGAAG
AAGAGGAAGG
TTGGCATCCA
CGGGGTGCCA
GCTGCTGACA
AGAAGTACTC
GATCGGCCTC
GCCATTGGGA
CTAACTCTGT
TGGCTGGGCC
GTGATCACCG
ACGAGTACAA
GGTGCCCTCA
AAGAAGTTCA
AGGTCCTGGG
CAACACCGAT
CGGCATTCCA
TCAAGAAGAA
TCTCATTGGC
GCTCTCCTGT
TCGACAGCGG
CGAGACGGCT
GAGGCTACGC
GGCTCAAGCG
CACCGCCCGC
AGGCGGTACA
CGCGCAGGAA
GAATCGCATC
TGCTACCTGC
AGGAGATTTT
CTCCAACGAG
ATGGCGAAGG
TTGACGATTC
TTTCTTCCAC
AGGCTGGAGG
AGTCATTCCT
CGTGGAGGAG
GATAAGAAGC
ACGAGCGGCA
TCCAATCTTC
GGCAACATTG
TCGACGAGGT
TGCCTACCAC
GAGAAGTACC
CTACGATCTA
CCATCTGCGG
AAGAAGCTCG
TGGACTCCAC
AGATAAGGCG
GACCTCCGCC
TGATCTACCT
CGCTCTGGCC
CACATGATTA
AGTTCAGGGG
CCATTTCCTG
ATCGAGGGGG
ATCTCAACCC
GGACAATAGC
GATGTTGACA
AGCTGTTCAT
CCAGCTCGTG
CAGACGTACA
ACCAGCTCTT
CGAGGAGAAC
CCCATTAATG
CGTCAGGCGT
CGACGCGAAG
GCTATCCTGT
CCGCTAGGCT
CTCGAAGTCT
CGGCGCCTCG
AGAACCTGAT
CGCCCAGCTG
CCGGGCGAGA
AGAAGAACGG
CCTGTTCGGG
AATCTCATTG
CGCTCAGCCT
GGGGCTCACG
CCCAACTTCA
AGTCGAATTT
CGATCTCGCT
GAGGACGCCA
AGCTGCAGCT
CTCCAAGGAC
ACATACGACG
ATGACCTGGA
TAACCTCCTG
GCCCAGATCG
GCGATCAGTA
CGCGGACCTG
TTCCTCGCTG
CCAAGAATCT
GTCGGACGCC
ATCCTCCTGT
CTGATATTCT
CAGGGTGAAC
ACCGAGATTA
CGAAGGCTCC
GCTCTCAGCC
TCCATGATCA
AGCGCTACGA
CGAGCACCAT
CAGGATCTGA
CCCTCCTGAA
GGCGCTGGTC
AGGCAGCAGC
TCCCCGAGAA
GTACAAGGAG
ATCTTCTTCG
ATCAGTCGAA
GAACGGCTAC
GCTGGGTACA
TTGACGGCGG
GGCCTCTCAG
GAGGAGTTCT
ACAAGTTCAT
CAAGCCGATT
CTGGAGAAGA
TGGACGGCAC
GGAGGAGCTG
CTGGTGAAGC
TCAATCGCGA
GGACCTCCTG
AGGAAGCAGC
GGACATTCGA
TAACGGCAGC
ATCCCACACC
AGATTCATCT
CGGGGAGCTG
CACGCTATCC
TGAGGAGGCA
GGAGGACTTC
TACCCTTTCC
TCAAGGATAA
CCGCGAGAAG
ATCGAGAAGA
TTCTGACTTT
CAGGATCCCG
TACTACGTCG
GCCCACTCGC
TAGGGGCAAC
TCCCGCTTCG
CTTGGATGAC
CCGCAAGTCA
GAGGAGACGA
TCACGCCGTG
GAACTTCGAG
GAGGTGGTCG
ACAAGGGCGC
TAGCGCTCAG
TCGTTCATCG
AGAGGATGAC
GAATTTCGAC
AAGAACCTGC
CAAATGAGAA
GGTGCTCCCT
AAGCACTCGC
TCCTGTACGA
GTACTTCACA
GTCTACAACG
AGCTGACTAA
GGTGAAGTAT
GTGACCGAGG
GCATGAGGAA
GCCGGCTTTC
CTGTCTGGGG
AGCAGAAGAA
GGCCATCGTG
GACCTCCTGT
TCAAGACCAA
CCGGAAGGTC
ACGGTTAAGC
AGCTCAAGGA
GGACTACTTC
AAGAAGATTG
AGTGCTTCGA
TTCGGTCGAG
ATCTCTGGCG
TTGAGGACCG
CTTCAACGCC
TCCCTGGGGA
CCTACCACGA
TCTCCTGAAG
ATCATTAAGG
ATAAGGACTT
CCTGGACAAC
GAGGAGAATG
AGGATATCCT
CGAGGACATT
GTGCTGACAC
TCACTCTGTT
CGAGGACCGG
GAGATGATCG
AGGAGCGCCT
GAAGACTTAC
GCCCATCTCT
TCGATGACAA
GGTCATGAAG
CAGCTCAAGA
GGAGGAGGTA
CACCGGCTGG
GGGAGGCTGA
GCAGGAAGCT
CATCAACGGC
ATTCGGGACA
AGCAGTCCGG
GAAGACGATC
CTCGACTTCC
TGAAGAGCGA
TGGCTTCGCG
AACCGCAATT
TCATGCAGCT
GATTCACGAT
GACAGCCTCA
CATTCAAGGA
GGATATCCAG
AAGGCTCAGG
TGAGCGGCCA
GGGGGACTCG
CTGCACGAGC
ATATCGCGAA
CCTCGCTGGC
TCGCCAGCTA
TCAAGAAGGG
GATTCTGCAG
ACCGTGAAGG
TTGTGGACGA
GCTGGTGAAG
GTCATGGGCA
GGCACAAGCC
TGAGAACATC
GTCATTGAGA
TGGCCCGGGA
GAATCAGACC
ACGCAGAAGG
GCCAGAAGAA
CTCACGCGAG
AGGATGAAGA
GGATCGAGGA
GGGCATTAAG
GAGCTGGGGT
CCCAGATCCT
CAAGGAGCAC
CCGGTGGAGA
ACACGCAGCT
GCAGAATGAG
AAGCTCTACC
TGTACTACCT
CCAGAATGGC
CGCGATATGT
ATGTGGACCA
GGAGCTGGAT
ATTAACAGGC
TCAGCGATTA
CGACGTCGAT
CATATCGTTC
CACAGTCATT
CCTGAAGGAT
GACTCCATTG
ACAACAAGGT
CCTCACCAGG
TCGGACAAGA
ACCGGGGCAA
GTCTGATAAT
GTTCCTTCAG
AGGAGGTCGT
TAAGAAGATG
AAGAACTACT
GGCGCCAGCT
CCTGAATGCC
AAGCTGATCA
CGCAGCGGAA
GTTCGATAAC
CTCACAAAGG
CTGAGAGGGG
CGGGCTCTCT
GAGCTGGACA
AGGCGGGCTT
CATCAAGAGG
CAGCTGGTCG
AGACACGGCA
GATCACTAAG
CACGTTGCGC
AGATTCTCGA
CTCACGGATG
AACACTAAGT
ACGATGAGAA
TGACAAGCTG
ATCCGCGAGG
TGAAGGTCAT
CACCCTGAAG
TCAAAGCTCG
TCTCCGACTT
CAGGAAGGAT
TTCCAGTTCT
ACAAGGTTCG
GGAGATCAAC
AATTACCACC
ATGCCCATGA
CGCGTACCTG
AACGCGGTGG
TCGGCACAGC
TCTGATCAAG
AAGTACCCAA
AGCTCGAGAG
CGAGTTCGTG
TACGGGGACT
ACAAGGTTTA
CGATGTGAGG
AAGATGATCG
CCAAGTCGGA
GCAGGAGATT
GGCAAGGCTA
CCGCCAAGTA
CTTCTTCTAC
TCTAACATTA
TGAATTTCTT
CAAGACAGAG
ATCACTCTGG
CCAATGGCGA
GATCCGGAAG
CGCCCCCTCA
TCGAGACGAA
CGGCGAGACG
GGGGAGATCG
TGTGGGACAA
GGGCAGGGAT
TTCGCGACCG
TCAGGAAGGT
TCTCTCCATG
CCACAAGTGA
ATATCGTCAA
GAAGACAGAG
GTCCAGACTG
GCGGGTTCTC
TAAGGAGTCA
ATTCTGCCTA
AGCGGAACAG
CGACAAGCTC
ATCGCCCGCA
AGAAGGACTG
GGATCCGAAG
AAGTACGGCG
GGTTCGACAG
CCCCACTGTG
GCCTACTCGG
TCCTGGTTGT
GGCGAAGGTT
GAGAAGGGCA
AGTCCAAGAA
GCTCAAGAGC
GTGAAGGAGC
TGCTGGGGAT
CACGATTATG
GAGCGCTCCA
GCTTCGAGAA
GAACCCGATC
GATTTCCTGG
AGGCGAAGGG
CTACAAGGAG
GTGAAGAAGG
ACCTGATCAT
TAAGCTCCCC
AAGTACTCAC
TCTTCGAGCT
GGAGAACGGC
AGGAAGCGGA
TGCTGGCTTC
CGCTGGCGAG
CTGCAGAAGG
GGAACGAGCT
GGCTCTGCCG
TCCAAGTATG
TGAACTTCCT
CTACCTGGCC
TCCCACTACG
AGAAGCTCAA
GGGCAGCCCC
GAGGACAACG
AGCAGAAGCA
GCTGTTCGTC
GAGCAGCACA
AGCATTACCT
CGACGAGATC
ATTGAGCAGA
TTTCCGAGTT
CTCCAAGCGC
GTGATCCTGG
CCGACGCGAA
TCTGGATAAG
GTCCTCTCCG
CGTACAACAA
GCACCGCGAC
AAGCCAATCA
GGGAGCAGGC
TGAGAATATC
ATTCATCTCT
TCACCCTGAC
GAACCTCGGC
GCCCCTGCTG
CTTTCAAGTA
CTTCGACACA
ACTATCGATC
GCAAGAGGTA
CACAAGCACT
AAGGAGGTCC
TGGACGCGAC
CCTCATCCAC
CAGTCGATTA
CCGGCCTCTA
CGAGACGCGC
ATCGACCTGT
CTCAGCTCGG
GGGCGACAAG
CGGCCAGCGG
CGACGAAGAA
GGCGGGGCAG
GCGAAGAAGA
AGAAGTGAGC
TCAGGCCTCC
ATACAAGTAT
TGGGGATCCA
CTAGCGAATT
TCCCCGATCG
TTCAAACATT
TGGCAATAAA
GTTTCTTAAG
ATTGAATCCT
GTTGCCGGTC
TTGCGATGAT
TATCATATAA
TTTCTGTTGA
ATTACGTTAA
GCATGTAATA
ATTAACATGT
AATGCATGAC
GTTATTTATG
AGATGGGTTT
TTATGATTAG
AGTCCCGCAA
TTATACATTT
AATACGCGAT
AGAAAACAAA
ATATAGCGCG
CAAACTAGGA
TAAATTATCG
CGCGCGGTGT
CATCTATGTT
ACTAGATCGG
AATTCGAGGC
GGTTTGCGTA
TTGGCTAGAG
CAGCTTGCCA
ACATGGTGGA
GCACGACACT
CTCGTCTACT
CCAAGAATAT
CAAAGATACA
GTCTCAGAAG
ACCAAAGGGC
TATTGAGACT
TTTCAACAAA
GGGTAATATC
GGGAAACCTC
CTCGGATTCC
ATTGCCCAGC
TATCTGTCAC
TTCATCAAAA
GGACAGTAGA
AAAGGAAGGT
GGCACCTACA
AATGCCATCA
TTGCGATAAA
GGAAAGGCTA
TCGTTCAAGA
TGCCTCTGCC
GACAGTGGTC
CCAAAGATGG
ACCCCCACCC
ACGAGGAGCA
TCGTGGAAAA
AGAAGACGTT
CCAACCACGT
CTTCAAAGCA
AGTGGATTGA
TGTGATAACA
TGGTGGAGCA
CGACACTCTC
GTCTACTCCA
AGAATATCAA
AGATACAGTC
TCAGAAGACC
AAAGGGCTAT
TGAGACTTTT
CAACAAAGGG
TAATATCGGG
AAACCTCCTC
GGATTCCATT
GCCCAGCTAT
CTGTCACTTC
ATCAAAAGGA
CAGTAGAAAA
GGAAGGTGGC
ACCTACAAAT
GCCATCATTG
CGATAAAGGA
AAGGCTATCG
TTCAAGATGC
CTCTGCCGAC
AGTGGTCCCA
AAGATGGACC
CCCACCCACG
AGGAGCATCG
TGGAAAAAGA
AGACGTTCCA
ACCACGTCTT
CAAAGCAAGT
GGATTGATGT
GATATCTCCA
CTGACGTAAG
GGATGACGCA
CAATCCCACT
ATCCTTCGCA
AGACCTTCCT
CTATATAAGG
AAGTTCATTT
CATTTGGAGA
GGACACGCTG
AAATCACCAG
TCTCTCTCTA
CAAATCTATC
TCTCTCGAGC
TTTCGCAGAT
CCCGGGGGGC
AATGAGATAT
GAAAAAGCCT
GAACTCACCG
CGACGTCTGT
CGAGAAGTTT
CTGATCGAAA
AGTTCGACAG
CGTCTCCGAC
CTGATGCAGC
TCTCGGAGGG
CGAAGAATCT
CGTGCTTTCA
GCTTCGATGT
AGGAGGGCGT
GGATATGTCC
TGCGGGTAAA
TAGCTGCGCC
GATGGTTTCT
ACAAAGATCG
TTATGTTTAT
CGGCACTTTG
CATCGGCCGC
GCTCCCGATT
CCGGAAGTGC
TTGACATTGG
GGAGTTTAGC
GAGAGCCTGA
CCTATTGCAT
CTCCCGCCGT
GCACAGGGTG
TCACGTTGCA
AGACCTGCCT
GAAACCGAAC
TGCCCGCTGT
TCTACAACCG
GTCGCGGAGG
CTATGGATGC
GATCGCTGCG
GCCGATCTTA
GCCAGACGAG
CGGGTTCGGC
CCATTCGGAC
CGCAAGGAAT
CGGTCAATAC
ACTACATGGC
GTGATTTCAT
ATGCGCGATT
GCTGATCCCC
ATGTGTATCA
CTGGCAAACT
GTGATGGACG
ACACCGTCAG
TGCGTCCGTC
GCGCAGGCTC
TCGATGAGCT
GATGCTTTGG
GCCGAGGACT
GCCCCGAAGT
CCGGCACCTC
GTGCACGCGG
ATTTCGGCTC
CAACAATGTC
CTGACGGACA
ATGGCCGCAT
AACAGCGGTC
ATTGACTGGA
GCGAGGCGAT
GTTCGGGGAT
TCCCAATACG
AGGTCGCCAA
CATCTTCTTC
TGGAGGCCGT
GGTTGGCTTG
TATGGAGCAG
CAGACGCGCT
ACTTCGAGCG
GAGGCATCCG
GAGCTTGCAG
GATCGCCACG
ACTCCGGGCG
TATATGCTCC
GCATTGGTCT
TGACCAACTC
TATCAGAGCT
TGGTTGACGG
CAATTTCGAT
GATGCAGCTT
GGGCGCAGGG
TCGATGCGAC
GCAATCGTCC
GATCCGGAGC
CGGGACTGTC
GGGCGTACAC
AAATCGCCCG
CAGAAGCGCG
GCCGTCTGGA
CCGATGGCTG
TGTAGAAGTA
CTCGCCGATA
GTGGAAACCG
ACGCCCCAGC
ACTCGTCCGA
GGGCAAAGAA
ATAGAGTAGA
TGCCGACCGG
ATCTGTCGAT
CGACAAGCTC
GAGTTTCTCC
ATAATAATGT
GTGAGTAGTT
CCCAGATAAG
GGAATTAGGG
TTCCTATAGG
GTTTCGCTCA
TGTGTTGAGC
ATATAAGAAA
CCCTTAGTAT
GTATTTGTAT
TTGTAAAATA
CTTCTATCAA
TAAAATTTCT
AATTCCTAAA
ACCAAAATCC
AGTACTAAAA
TCCAGATCCC
CCGAATTAAT
TCGGCGTTAA
TTCAGTACAT
TAAAAACGTC
CGCAATGTGT
TATTAAGTTG
TCACTAGTCA
GGTTAACTCA
ATTCGGCGTT
AATTCAGTAC
ATTAAAAACG
TCCGCAATGT
GTTATTAAGT
TGTCTAAGCG
TCAATTTGTT
TACACCACAA
TATATCCTGC
CACCAGCCAG
CCAACAGCTC
CCCGACCGGC
AGCTCGGCAC
AAAATCACCA
CTCGATACAG
GCAGCCCATC
AGTCCGGGAC
GGCGTCAGCG
GGAGAGCCGT
TGTAAGGCGG
CAGACTTTGC
TCATGTTACC
GATGCTATTC
GGAAGAACGG
CAACTAAGCT
GCCGGGTTTG
AAACACGGAT
GATCTCGCGG
AGGGTAGCAT
GTTGATTGTA
ACGATGACAG
AGCGTTGCTG
CCTGTGATCA
CTTAAGTAAC
TAACTAACAG
GAAGAGTTTG
TAGAAACGCA
AAAAGGCCAT
CCGTCAGGAT
GGCCTTCTGC
TTAGTTTGAT
GCCTGGCAGT
TTATGGCGGG
CGTCCTGCCC
GCCACCCTCC
GGGCCGTTGC
TTCACAACGT
TCAAATCCGC
TCCCGGCGGA
TTTGTCCTAC
TCAGGAGAGC
GTTCACCGAC
AAACAACAGA
TAAAACGAAA
GGCCCAGTCT
TCCGACTGAG
CCTTTCGTTT
TATTTGATGC
CTGGCAGTTC
CCTACTCTCG
CTTAGTAGTT
AGACGTCCCC
GAGATCCATG
CTAGACCATG
AATCCAGAAG
CCCGAGAGGT
TGCCGCCTTT
CGGGCTTTTT
CTTTTTCAAA
AAAAAAAATT
TATAAAACGA
TCTGTTGCGG
CCGGCCGCCG
GGTTGTGGGC
AAAGGCGCTC
GACGGTGGGC
AACCGCTTGC
GGTTGTCCAC
GGGCGGAGCC
GGTGCGCGTA
GCGCATTGTC
CACAAGCCAA
GGGCGACCAA
TAATTGATAT
ATATATTCAT
AATTGAAAAG
CTAATTGAAC
ATACTACTTG
CTGTAACTAC
TTGCCGGAGC
GAGGGGTGTT
TGCAAGCTGT
TGATCTGAAA
GGGCTATTAG
CGTTCTCACG
TGCCTTTTTG
ATTAGCGATT
TCACGTGACC
TTATTAGCGA
TTTCACGTAC
TCCGATTAGC
GATTTCACGT
ACCCTGATTA
GCGATTTCAC
GTGGATAGTT
TTTGGAGCGG
GCCGGAAAGC
CCCGTGAATC
AAGGCTTTGC
GGGGCATTAG
CGGTTTCACG
TGGATAACTA
CCCTCTATCC
ACAGGCTTCC
GGGGATAAAA
AAGCCCGCTC
GACGGCGGGC
TGTTGGATGG
GGATCTAGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGAACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACCGGG
TCTGACGCTC
AGTGGAACGG
GGCCCAATCT
GAATAATGTT
ACAACCAATT
AACCAATTCT
GATTAGAAAA
ACTCATCGAG
CATCAAATGA
AACTGCAATT
TATTCATATC
AGGATTATCA
ATACCATATT
TTTGAAAAAG
CCGTTTCTGT
AATGAAGGAG
AAAACTCACC
GAGGCAGTTC
CATAGGATGG
CAAGATCCTG
GTATCGGTCT
GCGATTCCGA
CTCGTCCAAC
ATCAATACAA
CCTATTAATT
TCCCCTCGTC
AAAAATAAGG
TTATCAAGTG
AGAAATCACC
ATGAGTGACG
ACTGAATCCG
GTGAGAATGG
CAAAAGTTTA
TGCATTTCTT
TCCAGACTTG
TTCAACAGGC
CAGCCATTAC
GCTCGTCATC
AAAATCACTC
GCATCAACCA
AACCGTTATT
CATTCGTGAT
TGCGCCTGAG
CGAGACGAAA
TACGCGATCG
CTGTTAAAAG
GACAATTACA
AACAGGAATC
GAATGCAACC
GGCGCAGGGA
CACTGCCAGC
GCATCAACAA
TATTTTCACC
TGAATCAGGA
TATTCTTCTA
ATACCTGGAA
TGCTGTTTTT
CCGGGGATCG
CAGTGGTGAG
TAACCATGCA
TCATCAGGAG
TACGGATAAA
ATGCTTGATG
GTCGGAAGAG
GCATAAATTC
CGTCAGCCAG
TTTAGTCTGA
CCATCTCATC
TGTAACATCA
TTGGCAACGC
TACCTTTGCC
ATGTTTCAGA
AACAACTCTG
GCGCATCGGG
CTTCCCATAC
AAGCGATAGA
TTGTCGCACC
TGATTGCCCG
ACATTATCGC
GAGCCCATTT
ATACCCATAT
AAATCAGCAT
CCATGTTGGA
ATTTAATCGC
GGCCTCGACG
TTTCCCGTTG
AATATGGCTC
ATAACACCCC
TTGTATTACT
GTTTATGTAA
GCAGACAGTT
TTATTGTTCA
TGATGATATA
TTTTTATCTT
GTGCAATGTA
ACATCAGAGA
TTTTGAGACA
CGGGCCAGAG
CTGCAGTTTG
ATCCCGAGGG
GAACCCTGTG
GTTGACATGC
ACATACAAAT
GGACGAACGG
ATAAACCTTT
TCACGCCCTT
TTAAATATCC
GTTATTCTAA
TAAACGCTCT
TTTCTCTTAG