产品货号：P33226
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pHCMC04-BmSQS-6×His |
|---|---|
| 别称: | |
| 基因长: | |
| 质粒宿主： | 枯草杆菌 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | |
| 原核抗性： | Amp |
| 筛选标记： | Chl |
| 荧光标记： | |
| 启动子: | xylA |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | DH5a |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
TCGAGGCCTG
CAGGCATGCA
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
GACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
ACTGCCGGGC
CTCTTGCGGG
ATCGGCGAGG
CTAGTTACCC
TTAAGTTATT
GGTATGACTG
GTTTTAAGCG
CAAAAAAAGT
TGCTTTTTCG
TACCTATTAA
TGTATCGTTT
TAGAAAACCG
ACTGTAAAAA
GTACAGTCGG
CATTATCTCA
TATTATAAAA
GCCAGTCATT
AGGCCTATCT
GACAATTCCT
GAATAGAGTT
CATAAACAAT
CCTGCATGAT
AACCATCACA
AACAGAATGA
TGTACCTGTA
AAGATAGCGG
TAAATATATT
GAATTACCTT
TATTAATGAA
TTTTCCTGCT
GTAATAATGG
GTAGAAGGTA
ATTACTATTA
TTATTGATAT
TTAAGTTAAA
CCCAGTAAAT
GAAGTCCATG
GAATAATAGA
AAGAGAAAAA
GCATTTTCAG
GTATAGGTGT
TTTGGGAAAC
AATTTCCCCG
AACCATTATA
TTTCTCTACA
TCAGAAAGGT
ATAAATCATA
AAACTCTTTG
AAGTCATTCT
TTACAGGAGT
CCAAATACCA
GAGAATGTTT
TAGATACACC
ATCAAAAATT
GTATAAAGTG
GCTCTAACTT
ATCCCAATAA
CCTAACTCTC
CGTCGCTATT
GTAACCAGTT
CTAAAAGCTG
TATTTGAGTT
TATCACCCTT
GTCACTAAGA
AAATAAATGC
AGGGTAAAAT
TTATATCCTT
CTTGTTTTAT
GTTTCGGTAT
AAAACACTAA
TATCAATTTC
TGTGGTTATA
CTAAAAGTCG
TTTGTTGGTT
CAAATAATGA
TTAAATATCT
CTTTTCTCTT
CCAATTGTCT
AAATCAATTT
TATTAAAGTT
CATTTGATAT
GCCTCCTAAA
TTTTTATCTA
AAGTGAATTT
AGGAGGCTTA
CTTGTCTGCT
TTCTTCATTA
GAATCAATCC
TTTTTTAAAA
GTCAATATTA
CTGTAACATA
AATATATATT
TTAAAAATAT
CCCACTTTAT
CCAATTTTCG
TTTGTTGAAC
TAATGGGTGC
TTTAGTTGAA
GAATAAAGAC
CACATTAAAA
AATGTGGTCT
TTTGTGTTTT
TTTAAAGGAT
TTGAGCGTAG
CGAAAAATCC
TTTTCTTTCT
TATCTTGATA
ATAAGGGTAA
CTATTGCCGA
TCGTCCATTC
CGACAGCATC
GCCAGTCACT
ATGGCGTGCT
GCTAGCGCCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGCTG
GCGAAAGGGG
GATGTGCTGC
AAGGCGATTA
AGTTGGGTAA
CGCCAGGGTT
TTCCCAGTCA
CGACGTTGTA
AAACGACGGC
CAGTGAATTC
GAGCTCCTAA
CTTATAGGGG
TAACACTTAA
AAAAGAATCA
ATAACGATAG
AAACCGCTCC
TAAAGCAGGT
GCATTTTTTC
CTAACGAAGA
AGGCAATAGT
TCACATTTAT
TGTCTAAATG
AGAATGGACT
CTAGAAGAAA
CTTCGTTTTT
AATCGTATTT
AAAACAATGG
GATGAGATTC
AATTATATGA
TTTCTCAAGA
TAACAGCTTC
TATATCAAAT
GTATTAAGGA
TATTGGTTAA
TCCAATTCCG
ATATAAAAGC
CAAAGTTTTG
AAGTGCATTT
AACATTTCTA
CATCATTTTT
ATTTGCGCGT
TCCACAATCT
CTTTTCGAGA
AATATTCTTT
TCTTCTTTAG
AGAGCGAAGC
CAGTAACGCT
TTTTCAGAAG
CATATAATTC
CCAACAGCCT
CGATTTCCAC
AGCTGCATTT
GGGTCCATTA
AAATCTATCG
TCATATGACC
CATTTCCCCA
GAAAAACCCT
GAACACCTTT
ATACAATTCG
TTGTTAATAA
CAAGTCCAGT
TCCAATTCCG
ATATTAATAC
TGATGTAAAC
GATGTTTTCA
TAGTTTTTTG
TCATACCAAA
TACTTTTTCA
CCGTATGCTC
CTGCATTAGC
TTCATTTTCA
ACAAAAACCG
GAACATTAAA
CTCACTCTCA
ATTAAAAACT
GCAAATCTTT
GATATTCCAA
TTTAAGTTAG
GCATGAAAAT
AATTTGCTGA
TGACGATCTA
CAAGGCCTGG
AACACAAATT
CCTATTCCGA
CTAGACCATA
AGGGGACTCA
GGCATATGGG
TTACAAAACC
ATGAATAAGT
GCAAATAAAA
TCTCTTTTAC
TTCACTAGCG
GAAGAACTAG
ACAAGTCAGA
AGTCTTCTCG
AGAATAATAT
TTCCTTCTAA
GTCGGTTAGA
ATTCCGTTAA
GATAGTCGAC
TCCTATATCA
ATACCAATCG
AGTAGCCTGC
ATTCTTATTA
AAAACAAGCA
TTACAGGTCT
TCTGCCGCCT
CTAGATTGCC
CTGCCCCAAT
TTCAAAAATA
AAATCTTTTT
CAAGCAGTGT
ATTTACTTGA
GAGGAGACAG
TAGACTTGTT
TAATCCTGTA
ATCTCAGAGA
GAGTTGCCCT
GGAGACAGGG
GAGTTCTTCA
AAATTTCATC
TAATATTAAT
TTTTGATTCA
TTTTTTTTAC
TAAAGCTTGA
TCTGCAATTT
GAATAATAAC
CACTCCTTTG
TTTATCCACC
GAACTAAGTT
GGTGTTTTTT
GAAGCTTGAA
TTAGATATTT
AAAAGTATCA
TATCTAATAT
TATAACTAAA
TTTTCTAAAA
AAAACATTGA
AATAAACATT
TATTTTGTAT
ATGATGAGAT
AAAGTTAGTT
TATTGGATAA
ACAAACTAAC
TCAATTAAGA
TAGTTGATGG
ATAAACTTGT
TCACTTAAAT
CAAAGGGGGA
AATGACAAAT
GGTCTAAACT
AGTAAGAGGA
GGAGAAATAT
GTCTGTTCCT
AACAAACTTC
GTGATAACGC
TATGGTTATG
CTTAAAGAAA
CATCTCGTAC
ATTCTTCATC
CCTATCTCTC
ATCTTCCTGC
TGAACTTCAA
AACGCTGTTG
GCTCTGCTTA
CCTTTGCATG
CGTGCTATCG
ATGAAATCGA
AGATCATCCT
GAACTTGAAG
CTGGCGTTAA
ATCTCGTCTT
CTTTACGCTA
TCTCTGATCT
TCTTAAAAAA
TCTTTCAACG
AAGATGAATA
CATGGCTCTT
GTTGGCCCTT
ACCAAGCTGA
TCTTCCTGAT
GTTACACTTC
AACTTGGCAA
CTGGATCGCT
CTTTGCCCTA
CAGGCGTTGC
TGATCAAGTT
CTTGATGCTA
CATCTATCAT
GGCTAAAGGC
ATGGCTGATT
GGGTTGAAAA
AGATTGGCAT
ATCCAAAACG
AAGCTGATCT
TGATGATTAC
ACATTCTACG
TTGCTGGCCT
TGTTGGCGTT
ATGCTTAACG
ATATCTGGAA
ATGGTACGAT
GGCACAGAAA
CAGATAAAGA
ACTTGCTATC
GCTTTCGGCC
GTGGCCTTCA
ATCTGTTAAC
ATCCTTCGTA
ACACATCTGA
AGATTCTGAA
CGTGGCGTTT
CTTTCTTCCC
TAACAACTGG
TCTCGTGAAG
ATATGTTCGC
TTACGCTCGT
CGTAACCTTT
CTCTTGGCAA
AAAATACCTT
GAAGATGTTA
AATCTCTTCC
TATCCTTCAT
TTCTGCAAAA
TCCCTCTTGC
TCTTGCTGAT
GGCACACTTA
ACGCTCTTAT
GAAAGGCAAA
GAAAAAATGA
CACGTGATGA
TGTTAACAAA
ACAGTTAACG
AAGTTGTTGA
TATGCATCAT
CATCATCATC
ATTAAGGATC
CTCTAGAGTC
GACGTCCCCG
GGGCAGCCCG
CCTAATGAGC
GGGCTTTTTT
CACGTCACGC
GTCCATGGAG
ATCTTTGTCT
GCAACTGAAA
AGTTTATACC
TTACCTGGAA
CAAATGGTTG
AAACATACGA
GGCTAATATC
GGCTTATTAG
GAATAGTCCC
TGTACTAATA
AAATCAGGTG
GATCAGTTGA
TCAGTATATT
TTGGACGAAG
CTCGGAAAGA
ATTTGGAGAT
GACTTGCTTA
ATTCCACAAT
TAAATTAAGG
GAAAGAATAA
AGCGATTTGA
TGTTCAAGGA
ATCACGGAAG
AAGATACTCA
TGATAAAGAA
GCTCTAAAAC
TATTCAATAA
CCTTACAATG
GAATTGATCG
AAAGGGTGGA
AGGTTAATGG
TACGAAAATT
AGGGGATCTA
CCTAGAAAGC
CACAAGGCGA
TAGGTCAAGC
TTAAAGAACC
CTTACATGGA
TCTTACAGAT
TCTGAAAGTA
AAGAAACAAC
AGAGGTTAAA
CAAACAGAAC
CAAAAAGAAA
AAAAGCATTG
TTGAAAACAA
TGAAAGTTGA
TGTTTCAATC
CATAATAAGA
TTAAATCGCT
GCACGAAATT
CTGGCAGCAT
CCGAAGGGAA
TTCATATTAC
TTAGAGGATA
CTATTGAGAG
AGCTATTGAT
AAGATGGTTG
AGACATTACC
TGAGAGCCAA
AAAACTTTTT
ATGAATATGA
ATTAAAAAAA
AGAACCAACA
AAGGCTGAGA
CAGACTCCAA
ACGAGTCTGT
TTTTTTAAAA
AAAATATTAG
GAGCATTGAA
TATATATTAG
AGAATTAAGA
AAGACATGGG
AATAAAAATA
TTTTAAATCC
AGTAAAAATA
TGATAAGATT
ATTTCAGAAT
ATGAAGAACT
CTGTTTGTTT
TTGATGAAAA
AACAAACAAA
AAAAATCCAC
CTAACGGAAT
CTCAATTTAA
CTAACAGCGG
CCAAACTGAG
AAGTTAAATT
TGAGAAGGGG
AAAAGGCGGA
TTTATACTTG
TATTTAACTA
TCTCCATTTT
AACATTTTAT
TAAACCCCAT
ACAAGTGAAA
ATCCTCTTTT
ACACTGTTCC
TTTAGGTGAT
CGCGGAGGGA
CATTATGAGT
GAAGTAAACC
TAAAAGGAAA
TACAGATGAA
TTAGTGTATT
ATCGACAGCA
AACCACTGGA
AATAAAATCG
CCAGGAAGAG
AATCAAAAAA
GGGAAAGAAG
AAGTTTATTA
TGTTGCTGAA
ACGGAAGAGA
AGATATGGAC
AGAAGAGCAA
ATAAAAAACT
TTTCTTTAGA
CAAATTTGGT
ACGCATATAC
CTTACATAGA
AGGTCATTAT
ACAATCTTAA
ATAATTACTT
CTTTGATTTT
TGGGGCTATT
TTTTAGGTGC
TGAAGGAATT
GCGCTCTATG
CTCACCTAAC
TCGTTATGCA
TACGGCAGCA
AAGACTTTTG
CTTTCCTAGT
CTACAAACAA
TCGCTAAAAA
AATGGACAAG
ACTCCTGTTA
CAGTTAGAGG
CTACTTGAAA
CTGCTTGAAA
GGTACGGTTT
TATTTGGAAG
GTAAACGTCC
GTAATAAAAC
CAAGGATAAC
ACAGAGGAAT
CCCCGATTTT
TAAGATTAGA
CGTAAGGTTC
CTTTGCTTTC
AGAAGAACTT
TTAAATGGAA
ACCCTAATAT
TGAAATTCCA
GATGACGAGG
AAGCACATGT
AAAGAAGGCT
TTAAAAAAGG
AAAAAGAGGG
TCTTCCAAAG
GTTTTGAAAA
AAGAGCACGA
TGAATTTGTT
AAAAAAATGA
TGGATGAGTC
AGAAACAATT
AATATTCCAG
AGGCCTTACA
ATATGACACA
ATGTATGAAG
ATATACTCAG
TAAAGGAGAA
ATTCGAAAAG
AAATCAAAAA
ACAAATACCT
AATCCTACAA
CATCTTTTGA
GAGTATATCA
ATGACAACTG
AAGAGGAAAA
AGTCGACAGT
ACTTTAAAAA
GCGAAATGCA
AAATCGTGTC
TCTAAGCCTT
CTTTTGATAC
CTGGTTTAAA
AACACTAAGA
TCAAAATTGA
AAATAAAAAT
TGTTTATTAC
TTGTACCGAG
TGAATTTGCA
TTTGAATGGA
TTAAGAAAAG
ATATTTAGAA
ACAATTAAAA
CAGTCCTTGA
AGAAGCTGGA
TATGTTTTCG
AAAAAATCGA
ACTAAGAAAA
GTGCAATAAA
CTGCTGAAGT
ATTTCAGCAG
TTTTTTTTAT
TTAGAAATAG
TGAAAAAAAT
ATAATCAGGG
AGGTATCAAT
ATTTAATGAG
TACTGATTTA
AATTTATTTA
GACTGGAATT
AATAATTAAC
ACGTAGACTA
ATTAAAATTT
AATGAGGGAT
AAAGAGGATA
CAAAAATATT
AATTTCAATC
CCTATTAAAT
TTTAACAAGG
GGGGGATTAA
AATTTAATTA
GAGGTTTATC
CACAAGAAAA
GACCCTAATA
AAATTTTTAC
TAGGGTTATA
ACACTGATTA
ATTTCTTAAT
GGGGGAGGGA
TTAAAATTTA
ATGACAAAGA
AAACAATCTT
TTAAGAAAAG
CTTTTAAAAG
ATAATAATAA
AAAGAGCTTT
GCGATTAAGC
AAAACTCTTT
ACTTTTTCAT
TGACATTATC
AAATTCATCG
ATTTCAAATT
GTTGTTGTAT
CATAAAGTTA
ATTCTGTTTT
GCACAACCTT
TTCAGGAATA
TAAAACACAT
CTGAGGCTTG
TTTTATAAAC
TCAGGGTCGC
TAAAGTCAAT
GTAACGTAGC
ATATGATATG
GTATAGCTTC
CACCCAAGTT
AGCCTTTCTG
CTTCTTCTGA
ATGTTTTTCA
TATACTTCCA
TGGGTATCTC
TAAATGATTT
TCCTCATGTA
GCAAGGTATG
AGCAAAAAGT
TTATGGAATT
GATAGTTCCT
CTCTTTTTCT
TCAACTTTTT
TATCTAAAAC
AAACACTTTA
ACATCTGAGT
CAATGTAAGC
ATAAGATGTT
TTTCCAGTCA
TAATTTCAAT
CCCAAATCTT
TTAGACAGAA
ATTCTGGACG
TAAATCTTTT
GGTGAAAGAA
TTTTTTTATG
TAGCAATATA
TCCGATACAG
CACCTTCTAA
AAGCGTTGGT
GAATAGGGCA
TTTTACCTAT
CTCCTCTCAT
TTTGTGGAAT
AAAAATAGTC
ATATTCGTCC
ATCTACCTAT
CCTATTATCG
AACAGTTGAA
CTTTTTAATC
AAGGATCAGT
CCTTTTTTTC
ATTATTCTTA
AACTGTGCTC
TTAACTTTAA
CAACTCGATT
TGTTTTTCCA
GATC
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