名称: | PYCm-PK9-T265A |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CACTGGCCGT
CGTTTTACAA
CGTCGTGACT
GGGAAAACCC
TGGCGTTACC
CAACTTAATC
GCCTTGCAGC
ACATCCCCCT
TTCGCCAGCT
GGCGTAATAG
CGAAGAGGCC
CGCACCGATC
GCCCTTCCCA
ACAGTTGCGC
AGCCTGAATG
GCGAATGGCG
CCACTATCCA
ACTACACTCA
TAATGCTTAA
AGAAAATTGG
CATTATTTTA
CCAATTTTTT
TAAAACAAAA
AAAGAATAAA
ATATTTTCTT
ATTATTTAGC
TAAAATTGTA
CTAAATTTTA
TACCAAAAAA
AATATATATA
AATATATGTA
ATATATAATA
TATATATATA
TATATGTATA
TATGTATGTA
TGTATATATG
TATGTATGTA
TATATGTATG
TATGTATATA
TGTATGTATG
TATGTATAGT
TTATATTTAT
TTGGCAAGTT
TTTCTGTAAA
AATGTTTAAT
TAAAAAAAAA
AATATATAAC
ATGCCAATAT
GCACTATTCA
TTTTATGGAG
GCAATTTTAT
GAGTAACCAT
TTTTACTTTT
TTTTTACATA
TGCGCATACT
TCGAGTTATA
CAATGTTATT
GGAGACGCGT
AGAGGCCTCG
TCTCAGTTTT
AGAGCTAGAA
ATAGCAAGTT
AAAATAAGGC
TAGTCCGTTA
TCAACTTGAA
AAAGTGGCAC
CGAGTCGGTG
CTTTTTTTGG
ACGTCAGGTG
GCACTTTTCG
GGGAAATGTG
CGCGGAACCC
CTATTTGTTT
ATTTTTCTAA
ATACATTCAA
ATATGTATCC
GCTCATGAGA
CAATAACCCT
GATAAATGCT
TCAATAATAT
TGAAAAAGGA
AGAGTATGAG
TATTCAACAT
TTCCGTGTCG
CCCTTATTCC
CTTTTTTGCG
GCATTTTGCC
TTCCTGTTTT
TGCTCACCCA
GAAACGCTGG
TGAAAGTAAA
AGATGCTGAA
GATCAGTTGG
GTGCACGAGT
GGGTTACATC
GAACTGGATC
TCAACAGCGG
TAAGATCCTT
GAGAGTTTTC
GCCCCGAAGA
ACGTTTTCCA
ATGATGAGCA
CTTTTAAAGT
TCTGCTATGT
GGCGCGGTAT
TATCCCGTAT
TGACGCCGGG
CAAGAGCAAC
TCGGTCGCCG
CATACACTAT
TCTCAGAATG
ACTTGGTTGA
GTACTCACCA
GTCACAGAAA
AGCATCTTAC
GGATGGCATG
ACAGTAAGAG
AATTATGCAG
TGCTGCCATA
ACCATGAGTG
ATAACACTGC
GGCCAACTTA
CTTCTGACAA
CGATCGGAGG
ACCGAAGGAG
CTAACCGCTT
TTTTGCACAA
CATGGGGGAT
CATGTAACTC
GCCTTGATCG
TTGGGAACCG
GAGCTGAATG
AAGCCATACC
AAACGACGAG
CGTGACACCA
CGATGCCTGT
AGCAATGGCA
ACAACGTTGC
GCAAACTATT
AACTGGCGAA
CTACTTACTC
TAGCTTCCCG
GCAACAATTA
ATAGACTGGA
TGGAGGCGGA
TAAAGTTGCA
GGACCACTTC
TGCGCTCGGC
CCTTCCGGCT
GGCTGGTTTA
TTGCTGATAA
ATCTGGAGCC
GGTGAGCGTG
GGTCTCGCGG
TATCATTGCA
GCACTGGGGC
CAGATGGTAA
GCCCTCCCGT
ATCGTAGTTA
TCTACACGAC
GGGGAGTCAG
GCAACTATGG
ATGAACGAAA
TAGACAGATC
GCTGAGATAG
GTGCCTCACT
GATTAAGCAT
TGGTAACTGT
CAGACCAAGT
TTACTCATAT
ATACTTTAGA
TTGATTTAAA
ACTTCATTTT
TAATTTAAAA
GGATCTAGGT
GAAGATCCTT
TTTGATAATC
TCATGACCAA
AATCCCTTAA
CGTGAGTTTT
CGTTCCACTG
AGCGTCAGAC
CCCGTAGAAA
AGATCAAAGG
ATCTTCTTGA
GATCCTTTTT
TTCTGCGCGT
AATCTGCTGC
TTGCAAACAA
AAAAACCACC
GCTACCAGCG
GTGGTTTGTT
TGCCGGATCA
AGAGCTACCA
ACTCTTTTTC
CGAAGGTAAC
TGGCTTCAGC
AGAGCGCAGA
TACCAAATAC
TGTCCTTCTA
GTGTAGCCGT
AGTTAGGCCA
CCACTTCAAG
AACTCTGTAG
CACCGCCTAC
ATACCTCGCT
CTGCTAATCC
TGTTACCAGT
GGCTGCTGCC
AGTGGCGATA
AGTCGTGTCT
TACCGGGTTG
GACTCAAGAC
GATAGTTACC
GGATAAGGCG
CAGCGGTCGG
GCTGAACGGG
GGGTTCGTGC
ACACAGCCCA
GCTTGGAGCG
AACGACCTAC
ACCGAACTGA
GATACCTACA
GCGTGAGCAT
TGAGAAAGCG
CCACGCTTCC
CGAAGGGAGA
AAGGCGGACA
GGTATCCGGT
AAGCGGCAGG
GTCGGAACAG
GAGAGCGCAC
GAGGGAGCTT
CCAGGGGGAA
ACGCCTGGTA
TCTTTATAGT
CCTGTCGGGT
TTCGCCACCT
CTGACTTGAG
CGTCGATTTT
TGTGATGCTC
GTCAGGGGGG
CGGAGCCTAT
GGAAAAACGC
CAGCAACGCG
GCCTTTTTAC
GGTTCCTGGC
CTTTTGCTGG
CCTTTTGCTC
ACATGTTCTT
TCCTGCGTTA
TCCCCTGATT
CTGTGGATAA
CCGTATTACC
GCCTTTGAGT
GAGCTGATAC
CGCTCGCCGC
AGCCGAACGA
CCGAGCGCAG
CGAGTCAGTG
AGCGAGGAAG
CGGAAGAGCG
CCCAATACGC
AAACCGCCTC
TCCCCGCGCG
TTGGCCGATT
CATTAATGCA
GCTGGCACGA
CAGGTTTCCC
GACTGGAAAG
CGGGCAGTGA
GCGCAACGCA
ATTAATGTGA
GTTAGCTCAC
TCATTAGGCA
CCCCAGGCTT
TACACTTTAT
GCTTCCGGCT
CGTATGTTGT
GTGGAATTGT
GAGCGGATAA
CAATTTCACA
CAGGAAACAG
CTATGACCAT
GATTACGCCA
AGCTTTGTTC
CTTTTGTTGT
AGACATATAA
TTGATCATTT
TATTTTTTTA
TTTATTATTT
TCCAAATTCT
TTAAATTTTT
ATTACCCTTA
AAAAATTTCC
CATTTTTCCA
TATAACCAAA
CTTAGCTAGT
TATGGCTAGT
TTTGTATGAC
CAATATTTTT
TAATATTCCT
AAGAATTTTT
TTTTTTAATT
TGTTTATAAA
ATTATGGAAA
ATCCAAGAAA
TCACAATTAT
CAATTTTTTA
GTTAATTTCA
CTTTTTATGT
TTTCAATAAT
TCCATCGAAA
AGACACGAAA
TGATATACTC
CCTTGCACTT
CACAAAAGTG
CGAAATAAAA
AATGTATAGT
AAATAATATA
ATATTAAATA
CGTAGTATTT
TTAGGCTTTC
CTATATTAAA
TTAATTATTC
TCGACATATT
TATTAATATA
TAGTAAGAAA
TGTAAATTTT
CCAACAAGCA
TATAACAATT
ACTGTTCATT
CAATATTGCC
ATCTAAAATG
GAATGGAATA
TATTTAAAGT
GGCTTCATAG
AAAGGGGATA
TTATTAAAGG
TATTATTTTT
AACTTTTTTT
TTTTTATTTA
ATTTTTAAAA
TTTATATTTT
AGTGCACTAT
ATCTATTCGT
GAAACATCGG
AGAAAATATA
TATATTCATA
TATTTAGATG
CACGTGGATG
CATATAAACG
TATTTACACA
CACCCTTTCG
TGTGAACTCT
GATTATTGAT
TCTATGAATT
GAATCATATC
AAGATGTATC
TAGGGAGTGT
CAAAAATGTG
GAGGATTTGT
TCCTGTCGAA
GGGAGAATAC
AAACAAGTAT
TCCTTTGTTT
AAAAAAAAAT
GAAAATAGTG
AGGCAATACA
AATAGATATA
AGCTGTGATA
GCAACATTTA
TAGTATCTTA
CAATTATGCA
GAGAAAAATA
TGGAATCCAT
GGTGATTATT
TAACAGATAG
TTGTGGAAAT
ATTGTAGAAA
ATATTAAAGA
GATAATAGAT
GGTGATACTT
TATATTTAAA
AGATAATAAA
GATAAATTTC
ATTTTTTAAA
TCAAATAAAA
TCAAATAATT
TAATAGTAAA
TGATTATATA
GTAGAAAAAA
GAATAGGGTC
AGGTGGATTT
GGTATAGTAT
TTCAAGGTAT
ACATATACAA
ACTAAACAAA
AAGTTGCACT
AAAATTTATA
CCTAAAAGTA
ATTTTTTAGA
TGTTACAGAT
GTACATAGAG
TCTTTATAGA
AATACAAACA
TTAAGAGGTT
TGATACATAA
AAATATAATT
AAAATGTATG
ATGTTAATCA
TTTTCAAAAT
TATGTATGTC
TAATTATGGA
ATATGCTGTT
AATAGTGATT
TAAAAAAATA
TATACAAAAA
AATAATGGAT
ATTTATCTGA
AAAAGAAACA
TATTTTTTAT
TTTTGCAAAT
TGTTAAAGGA
GTATATTATT
GTCATTCTAA
GCATATTGTA
CATAGAGATT
TAAAATTAGA
AAATATTTTA
CTTGATGAAA
ATATGACATG
TAAAATTGCA
GATTTTGGAT
TATCTGACTT
TGTTAATGTT
GATCAAAATA
TTAAAGCTGA
AGCTGGAACA
GAATTCCTTA
AGTTTACAAT
TTAATATTCA
TACTTTAAGT
ATTTTTTGTA
GTATCCTAGA
TATTGTGCTT
TAAATGCTCA
CCCCTCAAAG
CACCAGTAAT
ATTTTCATCC
ACTGAAATAC
CATTAAATTT
TCAAAAAAAT
ACTATGCATA
TAATGTTATA
CATATAAACA
TAAAACGCCA
TGTAAATCAA
AAAATATATA
AAAATATGTA
TAAAAATAAA
TATGCACTAA
ATATAAGCTA
ATTATGCATA
AAAATTAAAG
TGCCCTTTAT
TAACTAGTCG
TAATTATTTA
TATTTCTATG
TTATAAAAAA
ATCCTCATAT
AATAATATAA
TTAATATATG
TAATGTTTTT
TTTATTTTAT
AATTTTAATA
TAAAATAATA
TGTAAATTAA
TTCAAAAAAT
AAATATAATT
GTTGTGAAAC
AAAAAACGTA
ATTTTTTCAT
TTGCCTTCAA
AATTTAAATT
TATTTTAATA
TTTCCTAAAA
TATATATACT
TTGTGTATAA
ATATATAAAA
ATATATATTT
GCTTATAAAT
AAATAAAAAA
TTTTATAAAA
CATAGGGGGA
TCTATGGTTG
GTTCGCTAAA
CTGCATCGTC
GCTGTGTCCC
AGAACATGGG
CATCGGCAAG
AACGGGGACC
TGCCCTGGCC
ACCGCTCAGG
AACGAATTTA
GATATTTCCA
GAGAATGACC
ACAACCTCTT
CAGTAGAAGG
TAAACAGAAT
CTGGTGATTA
TGGGTAAGAA
GACCTGGTTC
TCCATTCCTG
AGAAGAATCG
ACCTTTAAAG
GGTAGAATTA
ATTTAGTTCT
CAGCAGAGAA
CTCAAGGAAC
CTCCACAAGG
AGCTCATTTT
CTTTCCAGAA
GTCTAGATGA
TGCCTTAAAA
CTTACTGAAC
AACCAGAATT
AGCAAATAAA
GTAGACATGG
TCTGGATAGT
TGGTGGCAGT
TCTGTTTATA
AGGAAGCCAT
GAATCACCCA
GGCCATCTTA
AACTATTTGT
GACAAGGATC
ATGCAAGACT
TTGAAAGTGA
CACGTTTTTT
CCAGAAATTG
ATTTGGAGAA
ATATAAACTT
CTGCCAGAAT
ACCCAGGTGT
TCTCTCTGAT
GTCCAGGAGG
AGAAAGGCAT
TAAGTACAAA
TTTGAAGTAT
ATGAGAAGAA
TGATGCAAGC
GGAGGAGGTG
GATCTGGTGG
AGGTGGAAGT
GCAAGCGTGA
CAGGGGGAAT
GGCTAGCAAG
TGGGATCAGA
AGGGTATGGA
CATTGCCTAT
GAGGAGGCGG
CCTTAGGTTA
CAAAGAGGGT
GGTGTTCCTA
TTGGCGGATG
TCTTATCAAT
AACAAAGACG
GAAGTGTTCT
CGGTCGTGGT
CACAACATGA
GATTTCAAAA
GGGATCCGCC
ACACTACATG
GTGAGATCTC
CACTTTGGAA
AACTGTGGGA
GATTAGAGGG
CAAAGTGTAC
AAAGATACCA
CTTTGTATAC
GACGCTGTCT
CCATGCGACA
TGTGTACAGG
TGCCATCATC
ATGTATGGTA
TTCCACGCTG
TGTTGTCGGT
GAGAACGTTA
ATTTCAAAAG
TAAGGGCGAG
AAATATTTAC
AAACTAGAGG
TCACGAGGTT
GTTGTTGTTG
ACGATGAGAG
GTGTAAAAAG
ATCATGAAAC
AATTTATCGA
TGAAAGACCT
CAGGATTGGT
TTGAAGATAT
TGGTGAGGCT
TCGGAACCAT
TTAAGAACGT
CTACTTGCTA
CCTCAAACAA
ACCAATTGCT
GGGTTTGTAC
ACCATCATCA
GAAATAAGAA
TACAACTAGA
CCTGATTTCA
TTTTCTACTC
CGATAGAATC
ATCAGATTGT
TGGTTGAAGA
AGGTTTGAAC
CATCTACCTG
TGCAAAAGCA
AATTGTGGAA
ACTGACACCA
ACGAAAACTT
CGAAGGTGTC
TCATTCATGG
GTAAAATCTG
TGGTGTTTCC
ATTGTCAGAG
CTGGTGAATC
GATGGAGCAA
GGATTAAGAG
ACTGTTGTAG
GTCTGTGCGT
ATCGGTAAAA
TTTTAATTCA
AAGGGACGAG
GAGACTGCTT
TACCAAAGTT
ATTCTACGAA
AAATTACCAG
AGGATATATC
TGAAAGGTAT
GTCTTCCTAT
TAGACCCAAT
GCTGGCCACC
GGTGGTAGTG
CTATCATGGC
TACAGAAGTC
TTGATTAAGA
GAGGTGTTAA
GCCAGAGAGA
ATTTACTTCT
TAAACCTAAT
CTGTAGTAAG
GAAGGGATTG
AAAAATACCA
TGCCGCCTTC
CCAGAGGTCA
GAATTGTTAC
TGGTGCCCTC
GACAGAGGTC
TAGATGAAAA
CAAGTATCTA
GTTCCAGGGT
TGGGTGACTT
TGGTGACAGA
TACTACTGTG
TTGGTTCGGG
AGAGGGCAGA
GGATCCCTGC
TAACATGCGG
TGATGTCGAG
GAGAATCCTG
GCCCAGAATC
GATGGACTAT
AAGGACCACG
ACGGAGACTA
CAAGGATCAT
GATATTGATT
ACAAAGACGA
TGACGATAAG
ATGGCCCCAA
AGAAGAAGCG
GAAGGTCGGT
ATCCACGGAG
TCCCAGCAGC
CGACAAGAAG
TACAGCATCG
GCCTGGACAT
CGGCACCAAC
TCTGTGGGCT
GGGCCGTGAT
CACCGACGAG
TACAAGGTGC
CCAGCAAGAA
ATTCAAGGTG
CTGGGCAACA
CCGACCGGCA
CAGCATCAAG
AAGAACCTGA
TCGGAGCCCT
GCTGTTCGAC
AGCGGCGAAA
CAGCCGAGGC
CACCCGGCTG
AAGAGAACCG
CCAGAAGAAG
ATACACCAGA
CGGAAGAACC
GGATCTGCTA
TCTGCAAGAG
ATCTTCAGCA
ACGAGATGGC
CAAGGTGGAC
GACAGCTTCT
TCCACAGACT
GGAAGAGTCC
TTCCTGGTGG
AAGAGGATAA
GAAGCACGAG
CGGCACCCCA
TCTTCGGCAA
CATCGTGGAC
GAGGTGGCCT
ACCACGAGAA
GTACCCCACC
ATCTACCACC
TGAGAAAGAA
ACTGGTGGAC
AGCACCGACA
AGGCCGACCT
GCGGCTGATC
TATCTGGCCC
TGGCCCACAT
GATCAAGTTC
CGGGGCCACT
TCCTGATCGA
GGGCGACCTG
AACCCCGACA
ACAGCGACGT
GGACAAGCTG
TTCATCCAGC
TGGTGCAGAC
CTACAACCAG
CTGTTCGAGG
AAAACCCCAT
CAACGCCAGC
GGCGTGGACG
CCAAGGCCAT
CCTGTCTGCC
AGACTGAGCA
AGAGCAGACG
GCTGGAAAAT
CTGATCGCCC
AGCTGCCCGG
CGAGAAGAAG
AATGGCCTGT
TCGGAAACCT
GATTGCCCTG
AGCCTGGGCC
TGACCCCCAA
CTTCAAGAGC
AACTTCGACC
TGGCCGAGGA
TGCCAAACTG
CAGCTGAGCA
AGGACACCTA
CGACGACGAC
CTGGACAACC
TGCTGGCCCA
GATCGGCGAC
CAGTACGCCG
ACCTGTTTCT
GGCCGCCAAG
AACCTGTCCG
ACGCCATCCT
GCTGAGCGAC
ATCCTGAGAG
TGAACACCGA
GATCACCAAG
GCCCCCCTGA
GCGCCTCTAT
GATCAAGAGA
TACGACGAGC
ACCACCAGGA
CCTGACCCTG
CTGAAAGCTC
TCGTGCGGCA
GCAGCTGCCT
GAGAAGTACA
AAGAGATTTT
CTTCGACCAG
AGCAAGAACG
GCTACGCCGG
CTACATTGAC
GGCGGAGCCA
GCCAGGAAGA
GTTCTACAAG
TTCATCAAGC
CCATCCTGGA
AAAGATGGAC
GGCACCGAGG
AACTGCTCGT
GAAGCTGAAC
AGAGAGGACC
TGCTGCGGAA
GCAGCGGACC
TTCGACAACG
GCAGCATCCC
CCACCAGATC
CACCTGGGAG
AGCTGCACGC
CATTCTGCGG
CGGCAGGAAG
ATTTTTACCC
ATTCCTGAAG
GACAACCGGG
AAAAGATCGA
GAAGATCCTG
ACCTTCCGCA
TCCCCTACTA
CGTGGGCCCT
CTGGCCAGGG
GAAACAGCAG
ATTCGCCTGG
ATGACCAGAA
AGAGCGAGGA
AACCATCACC
CCCTGGAACT
TCGAGGAAGT
GGTGGACAAG
GGCGCTTCCG
CCCAGAGCTT
CATCGAGCGG
ATGACCAACT
TCGATAAGAA
CCTGCCCAAC
GAGAAGGTGC
TGCCCAAGCA
CAGCCTGCTG
TACGAGTACT
TCACCGTGTA
TAACGAGCTG
ACCAAAGTGA
AATACGTGAC
CGAGGGAATG
AGAAAGCCCG
CCTTCCTGAG
CGGCGAGCAG
AAAAAGGCCA
TCGTGGACCT
GCTGTTCAAG
ACCAACCGGA
AAGTGACCGT
GAAGCAGCTG
AAAGAGGACT
ACTTCAAGAA
AATCGAGTGC
TTCGACTCCG
TGGAAATCTC
CGGCGTGGAA
GATCGGTTCA
ACGCCTCCCT
GGGCACATAC
CACGATCTGC
TGAAAATTAT
CAAGGACAAG
GACTTCCTGG
ACAATGAGGA
AAACGAGGAC
ATTCTGGAAG
ATATCGTGCT
GACCCTGACA
CTGTTTGAGG
ACAGAGAGAT
GATCGAGGAA
CGGCTGAAAA
CCTATGCCCA
CCTGTTCGAC
GACAAAGTGA
TGAAGCAGCT
GAAGCGGCGG
AGATACACCG
GCTGGGGCAG
GCTGAGCCGG
AAGCTGATCA
ACGGCATCCG
GGACAAGCAG
TCCGGCAAGA
CAATCCTGGA
TTTCCTGAAG
TCCGACGGCT
TCGCCAACAG
AAACTTCATG
CAGCTGATCC
ACGACGACAG
CCTGACCTTT
AAAGAGGACA
TCCAGAAAGC
CCAGGTGTCC
GGCCAGGGCG
ATAGCCTGCA
CGAGCACATT
GCCAATCTGG
CCGGCAGCCC
CGCCATTAAG
AAGGGCATCC
TGCAGACAGT
GAAGGTGGTG
GACGAGCTCG
TGAAAGTGAT
GGGCCGGCAC
AAGCCCGAGA
ACATCGTGAT
CGAAATGGCC
AGAGAGAACC
AGACCACCCA
GAAGGGACAG
AAGAACAGCC
GCGAGAGAAT
GAAGCGGATC
GAAGAGGGCA
TCAAAGAGCT
GGGCAGCCAG
ATCCTGAAAG
AACACCCCGT
GGAAAACACC
CAGCTGCAGA
ACGAGAAGCT
GTACCTGTAC
TACCTGCAGA
ATGGGCGGGA
TATGTACGTG
GACCAGGAAC
TGGACATCAA
CCGGCTGTCC
GACTACGATG
TGGACCATAT
CGTGCCTCAG
AGCTTTCTGA
AGGACGACTC
CATCGACAAC
AAGGTGCTGA
CCAGAAGCGA
CAAGAACCGG
GGCAAGAGCG
ACAACGTGCC
CTCCGAAGAG
GTCGTGAAGA
AGATGAAGAA
CTACTGGCGG
CAGCTGCTGA
ACGCCAAGCT
GATTACCCAG
AGAAAGTTCG
ACAATCTGAC
CAAGGCCGAG
AGAGGCGGCC
TGAGCGAACT
GGATAAGGCC
GGCTTCATCA
AGAGACAGCT
GGTGGAAACC
CGGCAGATCA
CAAAGCACGT
GGCACAGATC
CTGGACTCCC
GGATGAACAC
TAAGTACGAC
GAGAATGACA
AGCTGATCCG
GGAAGTGAAA
GTGATCACCC
TGAAGTCCAA
GCTGGTGTCC
GATTTCCGGA
AGGATTTCCA
GTTTTACAAA
GTGCGCGAGA
TCAACAACTA
CCACCACGCC
CACGACGCCT
ACCTGAACGC
CGTCGTGGGA
ACCGCCCTGA
TCAAAAAGTA
CCCTAAGCTG
GAAAGCGAGT
TCGTGTACGG
CGACTACAAG
GTGTACGACG
TGCGGAAGAT
GATCGCCAAG
AGCGAGCAGG
AAATCGGCAA
GGCTACCGCC
AAGTACTTCT
TCTACAGCAA
CATCATGAAC
TTTTTCAAGA
CCGAGATTAC
CCTGGCCAAC
GGCGAGATCC
GGAAGCGGCC
TCTGATCGAG
ACAAACGGCG
AAACCGGGGA
GATCGTGTGG
GATAAGGGCC
GGGATTTTGC
CACCGTGCGG
AAAGTGCTGA
GCATGCCCCA
AGTGAATATC
GTGAAAAAGA
CCGAGGTGCA
GACAGGCGGC
TTCAGCAAAG
AGTCTATCCT
GCCCAAGAGG
AACAGCGATA
AGCTGATCGC
CAGAAAGAAG
GACTGGGACC
CTAAGAAGTA
CGGCGGCTTC
GACAGCCCCA
CCGTGGCCTA
TTCTGTGCTG
GTGGTGGCCA
AAGTGGAAAA
GGGCAAGTCC
AAGAAACTGA
AGAGTGTGAA
AGAGCTGCTG
GGGATCACCA
TCATGGAAAG
AAGCAGCTTC
GAGAAGAATC
CCATCGACTT
TCTGGAAGCC
AAGGGCTACA
AAGAAGTGAA
AAAGGACCTG
ATCATCAAGC
TGCCTAAGTA
CTCCCTGTTC
GAGCTGGAAA
ACGGCCGGAA
GAGAATGCTG
GCCTCTGCCG
GCGAACTGCA
GAAGGGAAAC
GAACTGGCCC
TGCCCTCCAA
ATATGTGAAC
TTCCTGTACC
TGGCCAGCCA
CTATGAGAAG
CTGAAGGGCT
CCCCCGAGGA
TAATGAGCAG
AAACAGCTGT
TTGTGGAACA
GCACAAGCAC
TACCTGGACG
AGATCATCGA
GCAGATCAGC
GAGTTCTCCA
AGAGAGTGAT
CCTGGCCGAC
GCTAATCTGG
ACAAAGTGCT
GTCCGCCTAC
AACAAGCACC
GGGATAAGCC
CATCAGAGAG
CAGGCCGAGA
ATATCATCCA
CCTGTTTACC
CTGACCAATC
TGGGAGCCCC
TGCCGCCTTC
AAGTACTTTG
ACACCACCAT
CGACCGGAAG
AGGTACACCA
GCACCAAAGA
GGTGCTGGAC
GCCACCCTGA
TCCACCAGAG
CATCACCGGC
CTGTACGAGA
CACGGATCGA
CCTGTCTCAG
CTGGGAGGCG
ACAAAAGGCC
GGCGGCCACG
AAAAAGGCCG
GCCAGGCAAA
AAAGAAAAAG
TAAGAATTAC
CGGTGATCCC
GTTTTTCTTA
CTTATATATT
TATACCAATT
GATTGTATTT
ATAACTGTAA
AAATGTGTAT
GTTGTGTGCA
TATTTTTTTT
TGTGCATGCA
CATGCATGTA
AATAGCTAAA
ATTATGAACA
TTTTATTTTT
TGTTCAGAAA
AAAAAAACTT
TACACACATA
AAATGGCTAG
TATGAATAGC
CATATTTTAT
ATAAATTAAA
TCCTATGAAT
TTATGACCAT
ATTAAAAATT
TAGATATTTA
TGGAACATAA
TATGTTTGAA
ACAATAAGAC
AAAATTATTA
TTATTATTAT
TATTTTTACT
GTTATAATTA
TGTTGTCTCT
TCAATGATTC
ATAAATAGTT
GGACTTGATT
TTTAAAATGT
TTATAATATG
ATTAGCATAG
TTAAATAAAA
AAAGTTGAAA
AATTAAAAAA
AAACATATAA
ACACAAATGA
TGTTTTTTCC
TTCAATTTCG
G