名称: | pHDE-AtU6-ALKBH10B-sgRNA1-UBQ-ALKBH10B-sgRNA2-mCherry-Cas9 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 拟南芥 |
原核抗性: | Spe |
筛选标记: | Hyg |
荧光标记: | 红色 |
启动子: | 35S |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTTTACCCGC
CAATATATCC
TGTCAAACAC
TGATAGTTTA
AACTAGAGTG
TGCTGGAATT
GCCCTTAAGC
TTCGTTGAAC
AACGGAAACT
CGACTTGCCT
TCCGCACAAT
ACATCATTTC
TTCTTAGCTT
TTTTTCTTCT
TCTTCGTTCA
TACAGTTTTT
TTTTGTTTAT
CAGCTTACAT
TTTCTTGAAC
CGTAGCTTTC
GTTTTCTTCT
TTTTAACTTT
CCATTCGGAG
TTTTTGTATC
TTGTTTCATA
GTTTGTCCCA
GGATTAGAAT
GATTAGGCAT
CGAACCTTCA
AGAATTTGAT
TGAATAAAAC
ATCTTCATTC
TTAAGATATG
AAGATAATCT
TCAAAAGGCC
CCTGGGAATC
TGAAAGAAGA
GAAGCAGGCC
CATTTATATG
GGAAAGAACA
ATAGTATTTC
TTATATAGGC
CCATTTAAGT
TGAAAACAAT
CTTCAAAAGT
CCCACATCGC
TTAGATAAGA
AAACGAAGCT
GAGTTTATAT
ACAGCTAGAG
TCGAAGTAGT
GATTGGGATC
GATCTGTCTG
GCGTGCGTTT
TAGAGCTAGA
AATAGCAAGT
TAAAATAAGG
CTAGTCCGTT
ATCAACTTGA
AAAAGTGGCA
CCGAGTCGGT
GCTTTTTTTG
TCCCTTCGAA
GGGCAATTCT
GCAGATATCC
ATCACACTGG
TGAAGTGAAG
CTTGGTCTCA
ACTATGAGAC
CTAAGCTGGC
ACAACTATAT
TTCCAACATC
ACTAGCTACC
ATCAAAAGAT
TGACTTCTCA
TCTTACTCGA
TTGAAACCAA
ATTAACATAG
GGTTTTTATT
TAAATAAAAG
TTTAACCTTC
TTTTTAAAAA
ATTGTTCATA
GTGTCATGTC
AGAACAAGAG
CTACAAATCA
CACATAGCAT
GCATAAGCGG
AGCTATGATG
AGTGGTATTG
TTTTGTTCGT
CACTTGTCAC
TCTTTTCCAA
CACATAATCC
CGACAACAAC
GTAAGAGCAT
CTCTCTCTCT
CCACACACAC
TCATGCATGC
ATGCATTCTT
ACACGTGATT
GCCATGCAAA
TCTCCTTTCT
CACCTATAAA
TACAAACCAA
CCCTTCACTA
CACTCTTCAC
TCAAACCAAA
ACAAGAAAAC
ATACACAAAT
AGCAAAACGG
TACCAACAAT
GGATAACATG
GCCATCATCA
AGGAGTTCAT
GCGCTTCAAG
GTGCACATGG
AGGGCTCCGT
GAACGGCCAC
GAGTTCGAGA
TCGAGGGCGA
GGGCGAGGGC
CGCCCCTACG
AGGGCACCCA
GACCGCCAAG
CTGAAGGTGA
CCAAGGGTGG
CCCCCTGCCC
TTCGCCTGGG
ACATCCTGTC
CCCTCAGTTC
ATGTACGGCT
CCAAGGCCTA
CGTGAAGCAC
CCCGCCGACA
TCCCCGACTA
CTTGAAGCTG
TCCTTCCCCG
AGGGCTTCAA
GTGGGAGCGC
GTGATGAACT
TCGAGGACGG
CGGCGTGGTG
ACCGTGACCC
AGGACTCCTC
CCTGCAGGAC
GGCGAGTTCA
TCTACAAGGT
GAAGCTGCGC
GGCACCAACT
TCCCCTCCGA
CGGCCCCGTA
ATGCAGAAGA
AGACCATGGG
CTGGGAGGCC
TCCTCCGAGC
GGATGTACCC
CGAGGACGGC
GCCCTGAAGG
GCGAGATCAA
GCAGAGGCTG
AAGCTGAAGG
ACGGCGGCCA
CTACGACGCT
GAGGTCAAGA
CCACCTACAA
GGCCAAGAAG
CCCGTGCAGC
TGCCCGGCGC
CTACAACGTC
AACATCAAGT
TGGACATCAC
CTCCCACAAC
GAGGACTACA
CCATCGTGGA
ACAGTACGAA
CGCGCCGAGG
GCCGCCACTC
CACCGGCGGC
ATGGACGAGC
TGTACAAGTG
AGAGCTCTTG
GACTCCCATG
TTGGCAAAGG
CAACCAAACA
AACAATGAAT
GATCCGCTCC
TGCATATGGG
GCGGTTTGAG
TATTTCAACT
GCCATTTGGG
CTGAATTGAA
GACATGCTCC
TGTCAGAAAT
TCCGTGATCT
TACTCAATAT
TCAGTAATCT
CGGCCAATAT
CCTAAATGTG
CGTGGCTTTA
TCTGTCTTTG
TATTGTTTCA
TCAATTCATG
TAACGTTTGC
TTTTCTTATG
AATTTTCAAA
TAAATTATCG
TATTGAGACC
GAATTCTGTC
TAGCGTCGAC
GAGTCAGTAA
TAAACGGCGT
CAAAGTGGTT
GCAGCCGGCA
CACACGAGTC
GTGTTTATCA
ACTCAAAGCA
CAAATACTTT
TCCTCAACCT
AAAAATAAGG
CAATTAGCCA
AAAACAACTT
TGCGTGTAAA
CAACGCTCAA
TACACGTGTC
ATTTTATTAT
TAGCTATTGC
TTCACCGCCT
TAGCTTTCTC
GTGACCTAGT
CGTCCTCGTC
TTTTCTTCTT
CTTCTTCTAT
AAAACAATAC
CCAAAGAGCT
CTTCTTCTTC
ACAATTCAGA
TTTCAATTTC
TCAAAATCTT
AAAAACTTTC
TCTCAATTCT
CTCTACCGTG
ATCAAGGTAA
ATTTCTGTGT
TCCTTATTCT
CTCAAAATCT
TCGATTTTGT
TTTCGTTCGA
TCCCAATTTC
GTATATGTTC
TTTGGTTTAG
ATTCTGTTAA
TCTTAGATCG
AAGACGATTT
TCTGGGTTTG
ATCGTTAGAT
ATCATCTTAA
TTCTCGATTA
GGGTTTCATA
GATATCATCC
GATTTGTTCA
AATAATTTGA
GTTTTGTCGA
ATAATTACTC
TTCGATTTGT
GATTTCTATC
TAGATCTGGT
GTTAGTTTCT
AGTTTGTGCG
ATCGAATTTG
TCGATTAATC
TGAGTTTTTC
TGATTAACAG
CTCGCAATTG
ACCTAACTGA
GCTCCCTGAT
GAGTCCGTGA
GGACGAAACG
AGTAAGCTCG
TCGTTCAGAG
GAGAATTCGC
TGGTTTTAGA
GCTAGAAATA
GCAAGTTAAA
ATAAGGCTAG
TCCGTTATCA
ACTTGAAAAA
GTGGCACCGA
GTCGGTGCTT
TTGGCCGGCA
TGGTCCCAGC
CTCCTCGCTG
GCGCCGGCTG
GGCAACATGC
TTCGGCATGG
CGAATGGGAC
ACCTAACTGA
GTAAGCTAGC
TTGTTCGAGT
ATTATGGCAT
TGGGAAAACT
GTTTTTCTTG
TACCATTTGT
TGTGCTTGTA
ATTTACTGTG
TTTTTTATTC
GGTTTTCGCT
ATCGAACTGT
GAAATGGAAA
TGGATGGAGA
AGAGTTAATG
AATGATATGG
TCCTTTTGTT
CATTCTCAAA
TTAATATTAT
TTGTTTTTTC
TCTTATTTGT
TGTGTGTTGA
ATTTGAAATT
ATAAGAGATA
TGCAAACATT
TTGTTTTGAG
TAAAAATGTG
TCAAATCGTG
GCCTCTAATG
ACCGAAGTTA
ATATGAGGAG
TAAAACACTA
GATCCCCAAA
CAAGCTTGAA
ACTGAAGGCG
GGAAACGACA
ATCTGATCAT
GAGCGGAGAA
TTAAGGGAGT
CACGTTATGA
CCCCCGCCGA
TGACGCGGGA
CAAGCCGTTT
TACGTTTGGA
ACTGACAGAA
CCGCAACGTT
GAAGGAGCCA
CTCAGCCGCG
GGTTTCTGGA
GTTTAATGAG
CTAAGCACAT
ACGTCAGAAA
CCATTATTGC
GCGTTCAAAA
GTCGCCTAAG
GTCACTATCA
GCTAGCAAAT
ATTTCTTGTC
AAAAATGCTC
CACTGACGTT
CCATAAATTC
CCCTCGGTAT
CCAATTAGAG
TCTCATATTC
ACTCTCAATC
CAAATAATCT
GCACCGGATC
CGCTAGAGGA
TCTCGACCTG
CAAGATCCCG
GGGGGCAATG
AGATATGAAA
AAGCCTGAAC
TCACCGCGAC
GTCTGTCGAG
AAGTTTCTGA
TCGAAAAGTT
CGACAGCGTC
TCCGACCTGA
TGCAGCTCTC
GGAGGGCGAA
GAATCTCGTG
CTTTCAGCTT
CGATATAGGA
GGGCGTGGAT
ATGTCCTGCG
GGTAAATAGC
TGCGCCGATG
GTTTCTACAA
AGATCGTTAT
GTTTATCGGC
ACTTTGCATC
GGCCGCGCTC
CCGATTCCGG
AAGTGCTTGA
CATTGGGGAA
TTCAGCGAGA
GCCTGACCTA
TTGCATCTCC
CGCCGTGCAC
AGGGTGTCAC
GTTGCAAGAC
CTGCCTGAAA
CCGAACTGCC
CGCTGTTCTG
CAGCCGGTCG
CGGAGGCCAT
GGATGCGATC
GCTGCGGCCG
ATCTTAGCCA
GACGAGCGGG
TTCGGCCCAT
TCGGACCGCA
AGGAATCGGT
CAATACACTA
CATGGCGTGA
TTTCATATGC
GCGATTGCTG
ATCCCCATGT
GTATCACTGG
CAAACTGTGA
TGGACGACAC
CGTCAGTGCG
TCCGTCGCGC
AGGCTCTCGA
TGAGCTGATG
CTTTGGGCCG
AGGACTGCCC
CGAAGTCCGG
CACCTCGTGC
ACGCGGATTT
CGGCTCCAAC
AATGTCCTGA
CGGACAATGG
CCGCATAACA
GCGGTCATTG
ACTGGAGCGA
GGCGATGTTC
GGGGATTCCC
AATACGAGGT
CGCCAACATC
TTCTTCTGGA
GGCCGTGGTT
GGCTTGTATG
GAGCAGCAGA
CGCGCTACTT
CGAGCGGAGG
CATCCGGAGC
TTGCAGGATC
GCCGCGGCTC
CGGGCGTATA
TGCTCCGCAT
TGGTCTTGAC
CAACTCTATC
AGAGCTTGGT
TGACGGCAAT
TTCGATGATG
CAGCTTGGGC
GCAGGGTCGA
TGCGACGCAA
TCGTCCGATC
CGGAGCCGGG
ACTGTCGGGC
GTACACAAAT
CGCCCGCAGA
AGCGCGGCCG
TCTGGACCGA
TGGCTGTGTA
GAAGTACTCG
CCGATAGTGG
AAACCGACGC
CCCAGCACTC
GTCCGGGATC
TTGGAGGTGA
TGTAACATGA
TCACAAGCTG
ATCCCCCGAA
TTTCCCCGAT
CGTTCAAACA
TTTGGCAATA
AAGTTTCTTA
AGATTGAATC
CTGTTGCCGG
TCTTGCGATG
ATTATCATAT
AATTTCTGTT
GAATTACGTT
AAGCATGTAA
TAATTAACAT
GTAATGCATG
ACGTTATTTA
TGAGATGGGT
TTTTATGATT
AGAGTCCCGC
AATTATACAT
TTAATACGCG
ATAGAAAACA
AAATATAGCG
CGCAAACTAG
GATAAATTAT
CGCGCGCGGT
GTCATCTATG
TTACTAGATC
GGGAATTGAT
CCCCCCTCGA
CAGCTTGCAT
GCCAGCTTGG
GCTGCAGGTC
GACGCGTCAA
CATGGTGGAG
CACGACACTC
TCGTCTACTC
CAAGAATATC
AAAGATACAG
TCTCAGAAGA
CCAAAGGGCT
ATTGAGACTT
TTCAACAAAG
GGTAATATCG
GGAAACCTCC
TCGGATTCCA
TTGCCCAGCT
ATCTGTCACT
TCATCAAAAG
GACAGTAGAA
AAGGAAGGTG
GCACCTACAA
ATGCCATCAT
TGCGATAAAG
GAAAGGCTAT
CGTTCAAGAT
GCCTCTGCCG
ACAGTGGTCC
CAAAGATGGA
CCCCCACCCA
CGAGGAGCAT
CGTGGAAAAA
GAAGACGTTC
CAACCACGTC
TTCAAAGCAA
GTGGATTGAT
GTGATATCTC
CACTGACGTA
AGGGATGACG
CACAATCCCA
CTATCCTTCG
CAAGACCCTT
CCTCTATATA
AGGAAGTTCA
TTTCATTTGG
AGAGGACACG
ATATGGACAA
GAAGTACTCC
ATTGGGCTCG
ATATCGGCAC
AAACAGCGTC
GGCTGGGCCG
TCATTACGGA
CGAGTACAAG
GTGCCGAGCA
AAAAATTCAA
AGTTCTGGGC
AATACCGATC
GCCACAGCAT
AAAGAAGAAC
CTCATTGGCG
CCCTCCTGTT
CGACTCCGGG
GAGACGGCCG
AAGCCACGCG
GCTCAAAAGA
ACAGCACGGC
GCAGATATAC
CCGCAGAAAG
AATCGGATCT
GCTACCTGCA
GGAGATCTTT
AGTAATGAGA
TGGCTAAGGT
GGATGACTCT
TTCTTCCATA
GGCTGGAGGA
GTCCTTTTTG
GTGGAGGAGG
ATAAAAAGCA
CGAGCGCCAC
CCAATCTTTG
GCAATATCGT
GGACGAGGTG
GCGTACCATG
AAAAGTACCC
AACCATATAT
CATCTGAGGA
AGAAGCTTGT
AGACAGTACT
GATAAGGCTG
ACTTGCGGTT
GATCTATCTC
GCGCTGGCGC
ATATGATCAA
ATTTCGGGGA
CACTTCCTCA
TCGAGGGGGA
CCTGAACCCA
GACAACAGCG
ATGTCGACAA
ACTCTTTATC
CAACTGGTTC
AGACTTACAA
TCAGCTTTTC
GAAGAGAACC
CGATCAACGC
ATCCGGAGTT
GACGCCAAAG
CAATCCTGAG
CGCTAGGCTG
TCCAAATCCC
GGCGGCTCGA
AAACCTCATC
GCACAGCTCC
CTGGGGAGAA
GAAGAACGGC
CTGTTTGGTA
ATCTTATCGC
CCTGTCACTC
GGGCTGACCC
CCAACTTTAA
ATCTAACTTC
GACCTGGCCG
AAGATGCCAA
GCTTCAACTG
AGCAAAGACA
CCTACGATGA
TGATCTCGAC
AATCTGCTGG
CCCAGATCGG
CGACCAGTAC
GCAGACCTTT
TTTTGGCGGC
AAAGAACCTG
TCAGACGCCA
TTCTGCTGAG
TGATATTCTG
CGAGTGAACA
CGGAGATCAC
CAAAGCTCCG
CTGAGCGCTA
GTATGATCAA
GCGCTATGAT
GAGCACCACC
AAGACTTGAC
TTTGCTGAAG
GCCCTTGTCA
GACAGCAACT
GCCTGAGAAG
TACAAGGAAA
TTTTCTTCGA
TCAGTCTAAA
AATGGCTACG
CCGGATACAT
TGACGGCGGA
GCAAGCCAGG
AGGAATTTTA
CAAATTTATT
AAGCCCATCT
TGGAAAAAAT
GGACGGCACC
GAGGAGCTGC
TGGTAAAGCT
TAACAGAGAA
GATCTGTTGC
GCAAACAGCG
CACTTTCGAC
AATGGAAGCA
TCCCCCACCA
GATTCACCTG
GGCGAACTGC
ACGCTATCCT
CAGGCGGCAA
GAGGATTTCT
ACCCCTTTTT
GAAAGATAAC
AGGGAAAAGA
TTGAGAAAAT
CCTCACATTT
CGGATACCCT
ACTATGTAGG
CCCCCTCGCC
CGGGGAAATT
CCAGATTCGC
GTGGATGACT
CGCAAATCAG
AAGAGACCAT
CACTCCCTGG
AACTTCGAGG
AAGTCGTGGA
TAAGGGGGCC
TCTGCCCAGT
CCTTCATCGA
AAGGATGACT
AACTTTGATA
AAAATCTGCC
TAACGAAAAG
GTGCTTCCTA
AACACTCTCT
GCTGTACGAG
TACTTCACAG
TTTATAACGA
GCTCACCAAG
GTCAAATACG
TCACAGAAGG
GATGAGAAAG
CCAGCATTCC
TGTCTGGAGA
GCAGAAGAAA
GCTATCGTGG
ACCTCCTCTT
CAAGACGAAC
CGGAAAGTTA
CCGTGAAACA
GCTCAAAGAA
GACTATTTCA
AAAAGATTGA
ATGTTTCGAC
TCTGTTGAAA
TCAGCGGAGT
GGAGGATCGC
TTCAACGCAT
CCCTGGGAAC
GTATCACGAT
CTCCTGAAAA
TCATTAAAGA
CAAGGACTTC
CTGGACAATG
AGGAGAACGA
GGACATTCTT
GAGGACATTG
TCCTCACCCT
TACGTTGTTT
GAAGATAGGG
AGATGATTGA
AGAACGCTTG
AAAACTTACG
CTCATCTCTT
CGACGACAAA
GTCATGAAAC
AGCTCAAGAG
GCGCCGATAT
ACAGGATGGG
GGCGGCTGTC
AAGAAAACTG
ATCAATGGGA
TCCGAGACAA
GCAGAGTGGA
AAGACAATCC
TGGATTTTCT
TAAGTCCGAT
GGATTTGCCA
ACCGGAACTT
CATGCAGTTG
ATCCATGATG
ACTCTCTCAC
CTTTAAGGAG
GACATCCAGA
AAGCACAAGT
TTCTGGCCAG
GGGGACAGTC
TTCACGAGCA
CATCGCTAAT
CTTGCAGGTA
GCCCAGCTAT
CAAAAAGGGA
ATACTGCAGA
CCGTTAAGGT
CGTGGATGAA
CTCGTCAAAG
TAATGGGAAG
GCATAAGCCC
GAGAATATCG
TTATCGAGAT
GGCCCGAGAG
AACCAAACTA
CCCAGAAGGG
ACAGAAGAAC
AGTAGGGAAA
GGATGAAGAG
GATTGAAGAG
GGTATAAAAG
AACTGGGGTC
CCAAATCCTT
AAGGAACACC
CAGTTGAAAA
CACCCAGCTT
CAGAATGAGA
AGCTCTACCT
GTACTACCTG
CAGAACGGCA
GGGACATGTA
CGTGGATCAG
GAACTGGACA
TCAATCGGCT
CTCCGACTAC
GACGTGGATC
ATATCGTGCC
CCAGTCTTTT
CTCAAAGATG
ATTCTATTGA
TAATAAAGTG
TTGACAAGAT
CCGATAAAAA
TAGAGGGAAG
AGTGATAACG
TCCCCTCAGA
AGAAGTTGTC
AAGAAAATGA
AAAATTATTG
GCGGCAGCTG
CTGAACGCCA
AACTGATCAC
ACAACGGAAG
TTCGATAATC
TGACTAAGGC
TGAACGAGGT
GGCCTGTCTG
AGTTGGATAA
AGCCGGCTTC
ATCAAAAGGC
AGCTTGTTGA
GACACGCCAG
ATCACCAAGC
ACGTGGCCCA
AATTCTCGAT
TCACGCATGA
ACACCAAGTA
CGATGAAAAT
GACAAACTGA
TTCGAGAGGT
GAAAGTTATT
ACTCTGAAGT
CTAAGCTGGT
CTCAGATTTC
AGAAAGGACT
TTCAGTTTTA
TAAGGTGAGA
GAGATCAACA
ATTACCACCA
TGCGCATGAT
GCCTACCTGA
ATGCAGTGGT
AGGCACTGCA
CTTATCAAAA
AATATCCCAA
GCTTGAATCT
GAATTTGTTT
ACGGAGACTA
TAAAGTGTAC
GATGTTAGGA
AAATGATCGC
AAAGTCTGAG
CAGGAAATAG
GCAAGGCCAC
CGCTAAGTAC
TTCTTTTACA
GCAATATTAT
GAATTTTTTC
AAGACCGAGA
TTACACTGGC
CAATGGAGAG
ATTCGGAAGC
GACCACTTAT
CGAAACAAAC
GGAGAAACAG
GAGAAATCGT
GTGGGACAAG
GGTAGGGATT
TCGCGACAGT
CCGGAAGGTC
CTGTCCATGC
CGCAGGTGAA
CATCGTTAAA
AAGACCGAAG
TACAGACCGG
AGGCTTCTCC
AAGGAAAGTA
TCCTCCCGAA
AAGGAACAGC
GACAAGCTGA
TCGCACGCAA
AAAAGATTGG
GACCCCAAGA
AATACGGCGG
ATTCGATTCT
CCTACAGTCG
CTTACAGTGT
ACTGGTTGTG
GCCAAAGTGG
AGAAAGGGAA
GTCTAAAAAA
CTCAAAAGCG
TCAAGGAACT
GCTGGGCATC
ACAATCATGG
AGCGATCAAG
CTTCGAAAAA
AACCCCATCG
ACTTTCTCGA
GGCGAAAGGA
TATAAAGAGG
TCAAAAAAGA
CCTCATCATT
AAGCTTCCCA
AGTACTCTCT
CTTTGAGCTT
GAAAACGGCC
GGAAACGAAT
GCTCGCTAGT
GCGGGCGAGC
TGCAGAAAGG
TAACGAGCTG
GCACTGCCCT
CTAAATACGT
TAATTTCTTG
TATCTGGCCA
GCCACTATGA
AAAGCTCAAA
GGGTCTCCCG
AAGATAATGA
GCAGAAGCAG
CTGTTCGTGG
AACAACACAA
ACACTACCTT
GATGAGATCA
TCGAGCAAAT
AAGCGAATTC
TCCAAAAGAG
TGATCCTCGC
CGACGCTAAC
CTCGATAAGG
TGCTTTCTGC
TTACAATAAG
CACAGGGATA
AGCCCATCAG
GGAGCAGGCA
GAAAACATTA
TCCACTTGTT
TACTCTGACC
AACTTGGGCG
CGCCTGCAGC
CTTCAAGTAC
TTCGACACCA
CCATAGACAG
AAAGCGGTAC
ACCTCTACAA
AGGAGGTCCT
GGACGCCACA
CTGATTCATC
AGTCAATTAC
GGGGCTCTAT
GAAACAAGAA
TCGACCTCTC
TCAGCTCGGT
GGAGACAGCA
GGGCTGACCC
CAAGAAGAAG
AGGAAGGTGT
GATCGATCCA
GGCCTCCCAG
CTTTCGTCCG
TATCATCGGT
TTCGACAACG
TTCGTCAAGT
TCAATGCATC
AGTTTCATTG
CCCACACACC
AGAATCCTAC
TAAGTTTGAG
TATTATGGCA
TTGGAAAAGC
TGTTTTCTTC
TATCATTTGT
TCTGCTTGTA
ATTTACTGTG
TTCTTTCAGT
TTTTGTTTTC
GGACATCAAA
ATGCAAATGG
ATGGATAAGA
GTTAATAAAT
GATATGGTCC
TTTTGTTCAT
TCTCAAATTA
TTATTATCTG
TTGTTTTTAC
TTTAATGGGT
TGAATTTAAG
TAAGAAAGGA
ACTAACAGTG
TGATATTAAG
GTGCAATGTT
AGACATATAA
AACAGTCTTT
CACCTCTCTT
TGGTTATGTC
TTGAATTGGT
TTGTTTCTTC
ACTTATCTGT
GTAATCAAGT
TTACTATGAG
TCTATGATCA
AGTAATTATG
CAATCAAGTT
AAGTACAGTA
TAGGCTTTTT
GTGTCGAGGG
GGTACCGAGT
CGAGGAATTC
ACTGGCCGTC
GTTTTACAAC
GTCGTGACTG
GGAAAACCCT
GGCGTTACCC
AACTTAATCG
CCTTGCAGCA
CATCCCCCTT
TCGCCAGCTG
GCGTAATAGC
GAAGAGGCCC
GCACCGATCG
CCCTTCCCAA
CAGTTGCGCA
GCCTGAATGG
CGGGTACCGA
GCTCGAATTC
AATTCGGCGT
TAATTCAGTA
CATTAAAAAC
GTCCGCAATG
TGTTATTAAG
TTGTCTAAGC
GTCAATTTGT
TTACACCACA
ATATATCCTG
CCACCAGCCA
GCCAACAGCT
CCCCGACCGG
CAGCTCGGCA
CAAAATCACC
ACTCGATACA
GGCAGCCCAT
CAGTCCGGGA
CGGCGTCAGC
GGGAGAGCCG
TTGTAAGGCG
GCAGACTTTG
CTCATGTTAC
CGATGCTATT
CGGAAGAACG
GCAACTAAGC
TGCCGGGTTT
GAAACACGGA
TGATCTCGCG
GAGGGTAGCA
TGTTGATTGT
AACGATGACA
GAGCGTTGCT
GCCTGTGATC
AATTCGGGCA
CGAACCCAGT
GGACATAAGC
CTCGTTCGGT
TCGTAAGCTG
TAATGCAAGT
AGCGTAACTG
CCGTCACGCA
ACTGGTCCAG
AACCTTGACC
GAACGCAGCG
GTGGTAACGG
CGCAGTGGCG
GTTTTCATGG
CTTCTTGTTA
TGACATGTTT
TTTTGGGGTA
CAGTCTATGC
CTCGGGCATC
CAAGCAGCAA
GCGCGTTACG
CCGTGGGTCG
ATGTTTGATG
TTATGGAGCA
GCAACGATGT
TACGCAGCAG
GGCAGTCGCC
CTAAAACAAA
GTTAAACATC
ATGGGGGAAG
CGGTGATCGC
CGAAGTATCG
ACTCAACTAT
CAGAGGTAGT
TGGCGTCATC
GAGCGCCATC
TCGAACCGAC
GTTGCTGGCC
GTACATTTGT
ACGGCTCCGC
AGTGGATGGC
GGCCTGAAGC
CACACAGTGA
TATTGATTTG
CTGGTTACGG
TGACCGTAAG
GCTTGATGAA
ACAACGCGGC
GAGCTTTGAT
CAACGACCTT
TTGGAAACTT
CGGCTTCCCC
TGGAGAGAGC
GAGATTCTCC
GCGCTGTAGA
AGTCACCATT
GTTGTGCACG
ACGACATCAT
TCCGTGGCGT
TATCCAGCTA
AGCGCGAACT
GCAATTTGGA
GAATGGCAGC
GCAATGACAT
TCTTGCAGGT
ATCTTCGAGC
CAGCCACGAT
CGACATTGAT
CTGGCTATCT
TGCTGACAAA
AGCAAGAGAA
CATAGCGTTG
CCTTGGTAGG
TCCAGCGGCG
GAGGAACTCT
TTGATCCGGT
TCCTGAACAG
GATCTATTTG
AGGCGCTAAA
TGAAACCTTA
ACGCTATGGA
ACTCGCCGCC
CGACTGGGCT
GGCGATGAGC
GAAATGTAGT
GCTTACGTTG
TCCCGCATTT
GGTACAGCGC
AGTAACCGGC
AAAATCGCGC
CGAAGGATGT
CGCTGCCGAC
TGGGCAATGG
AGCGCCTGCC
GGCCCAGTAT
CAGCCCGTCA
TACTTGAAGC
TAGACAGGCT
TATCTTGGAC
AAGAAGAAGA
TCGCTTGGCC
TCGCGCGCAG
ATCAGTTGGA
AGAATTTGTC
CACTACGTGA
AAGGCGAGAT
CACCAAGGTA
GTCGGCAAAT
AATGTCTAGC
TAGAAATTCG
TTCAAGCCGA
CGCCGCTTCG
CCGGCGTTAA
CTCAAGCGAT
TAGATGCACT
AAGCACATAA
TTGCTCACAG
CCAAACTATC
AGGTCAAGTC
TGCTTTTATT
ATTTTTAAGC
GTGCATAATA
AGCCCTACAC
AAATTGGGAG
ATATATCATG
CATGACCAAA
ATCCCTTAAC
GTGAGTTTTC
GTTCCACTGA
GCGTCAGACC
CCGTAGAAAA
GATCAAAGGA
TCTTCTTGAG
ATCCTTTTTT
TCTGCGCGTA
ATCTGCTGCT
TGCAAACAAA
AAAACCACCG
CTACCAGCGG
TGGTTTGTTT
GCCGGATCAA
GAGCTACCAA
CTCTTTTTCC
GAAGGTAACT
GGCTTCAGCA
GAGCGCAGAT
ACCAAATACT
GTCCTTCTAG
TGTAGCCGTA
GTTAGGCCAC
CACTTCAAGA
ACTCTGTAGC
ACCGCCTACA
TACCTCGCTC
TGCTAATCCT
GTTACCAGTG
GCTGCTGCCA
GTGGCGATAA
GTCGTGTCTT
ACCGGGTTGG
ACTCAAGACG
ATAGTTACCG
GATAAGGCGC
AGCGGTCGGG
CTGAACGGGG
GGTTCGTGCA
CACAGCCCAG
CTTGGAGCGA
ACGACCTACA
CCGAACTGAG
ATACCTACAG
CGTGAGCTAT
GAGAAAGCGC
CACGCTTCCC
GAAGGGAGAA
AGGCGGACAG
GTATCCGGTA
AGCGGCAGGG
TCGGAACAGG
AGAGCGCACG
AGGGAGCTTC
CAGGGGGAAA
CGCCTGGTAT
CTTTATAGTC
CTGTCGGGTT
TCGCCACCTC
TGACTTGAGC
GTCGATTTTT
GTGATGCTCG
TCAGGGGGGC
GGAGCCTATG
GAAAAACGCC
AGCAACGCGG
CCTTTTTACG
GTTCCTGGCC
TTTTGCTGGC
CTTTTGCTCA
CATGTTCTTT
CCTGCGTTAT
CCCCTGATTC
TGTGGATAAC
CGTATTACCG
CCTTTGAGTG
AGCTGATACC
GCTCGCCGCA
GCCGAACGAC
CGAGCGCAGC
GAGTCAGTGA
GCGAGGAAGC
GGAAGAGCGC
CTGATGCGGT
ATTTTCTCCT
TACGCATCTG
TGCGGTATTT
CACACCGCAT
ATGGTGCACT
CTCAGTACAA
TCTGCTCTGA
TGCCGCATAG
TTAAGCCAGT
ATACACTCCG
CTATCGCTAC
GTGACTGGGT
CATGGCTGCG
CCCCGACACC
CGCCAACACC
CGCTGACGCG
CCCTGACGGG
CTTGTCTGCT
CCCGGCATCC
GCTTACAGAC
AAGCTGTGAC
CGTCTCCGGG
AGCTGCATGT
GTCAGAGGTT
TTCACCGTCA
TCACCGAAAC
GCGCGAGGCA
GGGTGCCTTG
ATGTGGGCGC
CGGCGGTCGA
GTGGCGACGG
CGCGGCTTGT
CCGCGCCCTG
GTAGATTGCC
TGGCCGTAGG
CCAGCCATTT
TTGAGCGGCC
AGCGGCCGCG
ATAGGCCGAC
GCGAAGCGGC
GGGGCGTAGG
GAGCGCAGCG
ACCGAAGGGT
AGGCGCTTTT
TGCAGCTCTT
CGGCTGTGCG
CTGGCCAGAC
AGTTATGCAC
AGGCCAGGCG
GGTTTTAAGA
GTTTTAATAA
GTTTTAAAGA
GTTTTAGGCG
GAAAAATCGC
CTTTTTTCTC
TTTTATATCA
GTCACTTACA
TGTGTGACCG
GTTCCCAATG
TACGGCTTTG
GGTTCCCAAT
GTACGGGTTC
CGGTTCCCAA
TGTACGGCTT
TGGGTTCCCA
ATGTACGTGC
TATCCACAGG
AAAGAGACCT
TTTCGACCTT
TTTCCCCTGC
TAGGGCAATT
TGCCCTAGCA
TCTGCTCCGT
ACATTAGGAA
CCGGCGGATG
CTTCGCCCTC
GATCAGGTTG
CGGTAGCGCA
TGACTAGGAT
CGGGCCAGCC
TGCCCCGCCT
CCTCCTTCAA
ATCGTACTCC
GGCAGGTCAT
TTGACCCGAT
CAGCTTGCGC
ACGGTGAAAC
AGAACTTCTT
GAACTCTCCG
GCGCTGCCAC
TGCGTTCGTA
GATCGTCTTG
AACAACCATC
TGGCTTCTGC
CTTGCCTGCG
GCGCGGCGTG
CCAGGCGGTA
GAGAAAACGG
CCGATGCCGG
GATCGATCAA
AAAGTAATCG
GGGTGAACCG
TCAGCACGTC
CGGGTTCTTG
CCTTCTGTGA
TCTCGCGGTA
CATCCAATCA
GCTAGCTCGA
TCTCGATGTA
CTCCGGCCGC
CCGGTTTCGC
TCTTTACGAT
CTTGTAGCGG
CTAATCAAGG
CTTCACCCTC
GGATACCGTC
ACCAGGCGGC
CGTTCTTGGC
CTTCTTCGTA
CGCTGCATGG
CAACGTGCGT
GGTGTTTAAC
CGAATGCAGG
TTTCTACCAG
GTCGTCTTTC
TGCTTTCCGC
CATCGGCTCG
CCGGCAGAAC
TTGAGTACGT
CCGCAACGTG
TGGACGGAAC
ACGCGGCCGG
GCTTGTCTCC
CTTCCCTTCC
CGGTATCGGT
TCATGGATTC
GGTTAGATGG
GAAACCGCCA
TCAGTACCAG
GTCGTAATCC
CACACACTGG
CCATGCCGGC
CGGCCCTGCG
GAAACCTCTA
CGTGCCCGTC
TGGAAGCTCG
TAGCGGATCA
CCTCGCCAGC
TCGTCGGTCA
CGCTTCGACA
GACGGAAAAC
GGCCACGTCC
ATGATGCTGC
GACTATCGCG
GGTGCCCACG
TCATAGAGCA
TCGGAACGAA
AAAATCTGGT
TGCTCGTCGC
CCTTGGGCGG
CTTCCTAATC
GACGGCGCAC
CGGCTGCCGG
CGGTTGCCGG
GATTCTTTGC
GGATTCGATC
AGCGGCCGCT
TGCCACGATT
CACCGGGGCG
TGCTTCTGCC
TCGATGCGTT
GCCGCTGGGC
GGCCTGCGCG
GCCTTCAACT
TCTCCACCAG
GTCATCACCC
AGCGCCGCGC
CGATTTGTAC
CGGGCCGGAT
GGTTTGCGAC
CGTCACGCCG
ATTCCTCGGG
CTTGGGGGTT
CCAGTGCCAT
TGCAGGGCCG
GCAGACAACC
CAGCCGCTTA
CGCCTGGCCA
ACCGCCCGTT
CCTCCACACA
TGGGGCATTC
CACGGCGTCG
GTGCCTGGTT
GTTCTTGATT
TTCCATGCCG
CCTCCTTTAG
CCGCTAAAAT
TCATCTACTC
ATTTATTCAT
TTGCTCATTT
ACTCTGGTAG
CTGCGCGATG
TATTCAGATA
GCAGCTCGGT
AATGGTCTTG
CCTTGGCGTA
CCGCGTACAT
CTTCAGCTTG
GTGTGATCCT
CCGCCGGCAA
CTGAAAGTTG
ACCCGCTTCA
TGGCTGGCGT
GTCTGCCAGG
CTGGCCAACG
TTGCAGCCTT
GCTGCTGCGT
GCGCTCGGAC
GGCCGGCACT
TAGCGTGTTT
GTGCTTTTGC
TCATTTTCTC
TTTACCTCAT
TAACTCAAAT
GAGTTTTGAT
TTAATTTCAG
CGGCCAGCGC
CTGGACCTCG
CGGGCAGCGT
CGCCCTCGGG
TTCTGATTCA
AGAACGGTTG
TGCCGGCGGC
GGCAGTGCCT
GGGTAGCTCA
CGCGCTGCGT
GATACGGGAC
TCAAGAATGG
GCAGCTCGTA
CCCGGCCAGC
GCCTCGGCAA
CCTCACCGCC
GATGCGCGTG
CCTTTGATCG
CCCGCGACAC
GACAAAGGCC
GCTTGTAGCC
TTCCATCCGT
GACCTCAATG
CGCTGCTTAA
CCAGCTCCAC
CAGGTCGGCG
GTGGCCCATA
TGTCGTAAGG
GCTTGGCTGC
ACCGGAATCA
GCACGAAGTC
GGCTGCCTTG
ATCGCGGACA
CAGCCAAGTC
CGCCGCCTGG
GGCGCTCCGT
CGATCACTAC
GAAGTCGCGC
CGGCCGATGG
CCTTCACGTC
GCGGTCAATC
GTCGGGCGGT
CGATGCCGAC
AACGGTTAGC
GGTTGATCTT
CCCGCACGGC
CGCCCAATCG
CGGGCACTGC
CCTGGGGATC
GGAATCGACT
AACAGAACAT
CGGCCCCGGC
GAGTTGCAGG
GCGCGGGCTA
GATGGGTTGC
GATGGTCGTC
TTGCCTGACC
CGCCTTTCTG
GTTAAGTACA
GCGATAACCT
TCATGCGTTC
CCCTTGCGTA
TTTGTTTATT
TACTCATCGC
ATCATATACG
CAGCGACCGC
ATGACGCAAG
CTGTTTTACT
CAAATACACA
TCACCTTTTT
AGACGGCGGC
GCTCGGTTTC
TTCAGCGGCC
AAGCTGGCCG
GCCAGGCCGC
CAGCTTGGCA
TCAGACAAAC
CGGCCAGGAT
TTCATGCAGC
CGCACGGTTG
AGACGTGCGC
GGGCGGCTCG
AACACGTACC
CGGCCGCGAT
CATCTCCGCC
TCGATCTCTT
CGGTAATGAA
AAACGGTTCG
TCCTGGCCGT
CCTGGTGCGG
TTTCATGCTT
GTTCCTCTTG
GCGTTCATTC
TCGGCGGCCG
CCAGGGCGTC
GGCCTCGGTC
AATGCGTCCT
CACGGAAGGC
ACCGCGCCGC
CTGGCCTCGG
TGGGCGTCAC
TTCCTCGCTG
CGCTCAAGTG
CGCGGTACAG
GGTCGAGCGA
TGCACGCCAA
GCAGTGCAGC
CGCCTCTTTC
ACGGTGCGGC
CTTCCTGGTC
GATCAGCTCG
CGGGCGTGCG
CGATCTGTGC
CGGGGTGAGG
GTAGGGCGGG
GGCCAAACTT
CACGCCTCGG
GCCTTGGCGG
CCTCGCGCCC
GCTCCGGGTG
CGGTCGATGA
TTAGGGAACG
CTCGAACTCG
GCAATGCCGG
CGAACACGGT
CAACACCATG
CGGCCGGCCG
GCGTGGTGGT
GTCGGCCCAC
GGCTCTGCCA
GGCTACGCAG
GCCCGCGCCG
GCCTCCTGGA
TGCGCTCGGC
AATGTCCAGT
AGGTCGCGGG
TGCTGCGGGC
CAGGCGGTCT
AGCCTGGTCA
CTGTCACAAC
GTCGCCAGGG
CGTAGGTGGT
CAAGCATCCT
GGCCAGCTCC
GGGCGGTCGC
GCCTGGTGCC
GGTGATCTTC
TCGGAAAACA
GCTTGGTGCA
GCCGGCCGCG
TGCAGTTCGG
CCCGTTGGTT
GGTCAAGTCC
TGGTCGTCGG
TGCTGACGCG
GGCATAGCCC
AGCAGGCCAG
CGGCGGCGCT
CTTGTTCATG
GCGTAATGTC
TCCGGTTCTA
GTCGCAAGTA
TTCTACTTTA
TGCGACTAAA
ACACGCGACA
AGAAAACGCC
AGGAAAAGGG
CAGGGCGGCA
GCCTGTCGCG
TAACTTAGGA
CTTGTGCGAC
ATGTCGTTTT
CAGAAGACGG
CTGCACTGAA
CGTCAGAAGC
CGACTGCACT
ATAGCAGCGG
AGGGGTTGGA
TCAAAGTACT
TTAAAGTACT
TTAAAGTACT
TTAAAGTACT
TTGATCCCGA
GGGGAACCCT
GTGGTTGGCA
TGCACATACA
AATGGACGAA
CGGATAAACC
TTTTCACGCC
CTTTTAAATA
TCCGATTATT
CTAATAAACG
CTCTTTTCTC
TTAG