名称: | pKI1.1R-AtU6-BRI1-sgRNA1-RPS5A-Cas9 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Spe |
筛选标记: | Hyg |
荧光标记: | 红色 |
启动子: | AtU6-26 |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGACGTCGCA
TGCTCCCGGC
CGCCATGGCC
GCGGGATATC
ACAAGTTTGT
ACAAAAAAGC
AGGCTCCGCG
GCCGCTCGTT
GAACAACGGA
AACTCGACTT
GCCTTCCGCA
CAATACATCA
TTTCTTCTTA
GCTTTTTTTC
TTCTTCTTCG
TTCATACAGT
TTTTTTTTGT
TTATCAGCTT
ACATTTTCTT
GAACCGTAGC
TTTCGTTTTC
TTCTTTTTAA
CTTTCCATTC
GGAGTTTTTG
TATCTTGTTT
CATAGTTTGT
CCCAGGATTA
GAATGATTAG
GCATCGAACC
TTCAAGAATT
TGATTGAATA
AAACATCTTC
ATTCTTAAGA
TATGAAGATA
ATCTTCAAAA
GGCCCCTGGG
AATCTGAAAG
AAGAGAAGCA
GGCCCATTTA
TATGGGAAAG
AACAATAGTA
TTTCTTATAT
AGGCCCATTT
AAGTTGAAAA
CAATCTTCAA
AAGTCCCACA
TCGCTTAGAT
AAGAAAACGA
AGCTGAGTTT
ATATACAGCT
AGAGTCGAAG
TAGTGATTGT
TGGGTCATAA
CGATATCTCG
TTTTAGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTATCAACT
TGAAAAAGTG
GCACCGAGTC
GGTGCTTTTT
TTTTTTGCGG
CCGCGATCCC
TCAACTTTTG
ATTCGCTATT
TGCAGTGCAC
CTGTGGCGTT
CATCACATCT
TTTGTGACAC
TGTTTGCACT
GGTCATTGCT
ATTACAAAGG
ACCTTCCTGA
TGTTGAAGGA
GATCGAAAGT
AAGTAACTGC
ACGCATAACC
ATTTTCTTTC
CGCTCTTTGG
CTCAATCCAT
TTGACAGTCA
AAGACAATGT
TTAACCAGCT
CCGTTTGATA
TATTGTCTTT
ATGTGTTTGT
TCAAGCATGT
TTAGTTAATC
ATGCCTTTGA
TTGATCTTGA
ATAGGTTCCA
AATATCAACC
CTGGCAACAA
AACTTGGAGT
GAGAAACATT
GCATTCCTCG
GTTCTGGACT
TCTGCTAGTA
AATTATGTTT
CAGCCATATC
ACTAGCTTTC
TACATGCCTC
AGGTGAATTC
ATCTATTTCC
GTCTTAACTA
TTTCGGTTAA
TTAAAGCACG
AACACCATTA
CTGCATGTAG
AAGCTTGATA
AACTATCGCC
ACCAATTTAT
TTTTGTTGCG
ATATTGTTAC
TTTCCTCAGT
ATGCAGCTTT
GAAAAGACCA
ACCCTCTTAT
CCTTTAACAA
TGAACAGGTT
TTTAGAGGTA
GCTTGATGAT
TCCTGCACAT
GTGATCTTGG
CTTCAGGCTT
AATTTTCCAG
GTAAAGCATT
ATGAGATACT
CTTATATCTC
TTACATACTT
TTGAGATAAT
GCACAAGAAC
TTCATAACTA
TATGCTTTAG
TTTCTGCATT
TGACACTGCC
AAATTCATTA
ATCTCTAATA
TCTTTGTTGT
TGATCTTTGG
TAGACATGGG
TACTAGAAAA
AGCAAACTAC
ACCAAGGTAA
AATACTTTTG
TACAAACATA
AACTCGTTAT
CACGGAACAT
CAATGGAGTG
TATATCTAAC
GGAGTGTAGA
AACATTTGAT
TATTGCAGGA
AGCTATCTCA
GGATATTATC
GGTTTATATG
GAATCTCTTC
TACGCAGAGT
ATCTGTTATT
CCCCTTCCTC
TAGCTTTCAA
TTTCATGGTG
AGGATATGCA
GTTTTCTTTG
TATATCATTC
TTCTTCTTCT
TTGTAGCTTG
GAGTCAAAAT
CGGTTCCTTC
ATGTACATAC
ATCAAGGATA
TGTCCTTCTG
AATTTTTATA
TCTTGCAATA
AAAATGCTTG
TACCAATTGA
AACACCAGCT
TTTTGAGTTC
TATGATCACT
GACTTGGTTC
TAACCAAAAA
AAAAAAATGT
TTAATTTACA
TATCTAAAAG
TAGGTTTAGG
GAAACCTAAA
CAGTAAAATA
TTTGTATATT
ATTCGAATTT
CACTCATCAT
AAAAACTTAA
ATTGCACCAT
AAAATTTTGT
TTTACTATTA
ATGATGTAAT
TTGTGTAACT
TAAGATAAAA
ATAATATTCC
GTAAGTTAAC
CGGCTAAAAC
CACGTATAAA
CCAGGGAACC
TGTTAAACCG
GTTCTTTACT
GGATAAAGAA
ATGAAAGCCC
ATGTAGACAG
CTCCATTAGA
GCCCAAACCC
TAAATTTCTC
ATCTATATAA
AAGGAGTGAC
ATTAGGGTTT
TTGTTCGTCC
TCTTAAAGCT
TCTCGTTTTC
TCTGCCGTCT
CTCTCATTCG
CGCGACGCAA
ACGATCTTCA
GGTGATCTTC
TTTCTCCAAA
TCCTCTCTCA
TAACTCTGAT
TTCGTACTTG
TGTATTTGAG
CTCACGCTCT
GTTTCTCTCA
CCACAGCCCC
ATGGACTATA
AGGACCACGA
CGGAGACTAC
AAGGATCATG
ATATTGATTA
CAAAGACGAT
GACGATAAGA
TGGCCCCAAA
GAAGAAGCGG
AAGGTCGGTA
TCCACGGAGT
CCCAGCAGCC
GACAAGAAGT
ACAGCATCGG
CCTGGACATC
GGCACCAACT
CTGTGGGCTG
GGCCGTGATC
ACCGACGAGT
ACAAGGTGCC
CAGCAAGAAA
TTCAAGGTGC
TGGGCAACAC
CGACCGGCAC
AGCATCAAGA
AGAACCTGAT
CGGAGCCCTG
CTGTTCGACA
GCGGCGAAAC
AGCCGAGGCC
ACCCGGCTGA
AGAGAACCGC
CAGAAGAAGA
TACACCAGAC
GGAAGAACCG
GATCTGCTAT
CTGCAAGAGA
TCTTCAGCAA
CGAGATGGCC
AAGGTGGACG
ACAGCTTCTT
CCACAGACTG
GAAGAGTCCT
TCCTGGTGGA
AGAGGATAAG
AAGCACGAGC
GGCACCCCAT
CTTCGGCAAC
ATCGTGGACG
AGGTGGCCTA
CCACGAGAAG
TACCCCACCA
TCTACCACCT
GAGAAAGAAA
CTGGTGGACA
GCACCGACAA
GGCCGACCTG
CGGCTGATCT
ATCTGGCCCT
GGCCCACATG
ATCAAGTTCC
GGGGCCACTT
CCTGATCGAG
GGCGACCTGA
ACCCCGACAA
CAGCGACGTG
GACAAGCTGT
TCATCCAGCT
GGTGCAGACC
TACAACCAGC
TGTTCGAGGA
AAACCCCATC
AACGCCAGCG
GCGTGGACGC
CAAGGCCATC
CTGTCTGCCA
GACTGAGCAA
GAGCAGACGG
CTGGAAAATC
TGATCGCCCA
GCTGCCCGGC
GAGAAGAAGA
ATGGCCTGTT
CGGAAACCTG
ATTGCCCTGA
GCCTGGGCCT
GACCCCCAAC
TTCAAGAGCA
ACTTCGACCT
GGCCGAGGAT
GCCAAACTGC
AGCTGAGCAA
GGACACCTAC
GACGACGACC
TGGACAACCT
GCTGGCCCAG
ATCGGCGACC
AGTACGCCGA
CCTGTTTCTG
GCCGCCAAGA
ACCTGTCCGA
CGCCATCCTG
CTGAGCGACA
TCCTGAGAGT
GAACACCGAG
ATCACCAAGG
CCCCCCTGAG
CGCCTCTATG
ATCAAGAGAT
ACGACGAGCA
CCACCAGGAC
CTGACCCTGC
TGAAAGCTCT
CGTGCGGCAG
CAGCTGCCTG
AGAAGTACAA
AGAGATTTTC
TTCGACCAGA
GCAAGAACGG
CTACGCCGGC
TACATTGACG
GCGGAGCCAG
CCAGGAAGAG
TTCTACAAGT
TCATCAAGCC
CATCCTGGAA
AAGATGGACG
GCACCGAGGA
ACTGCTCGTG
AAGCTGAACA
GAGAGGACCT
GCTGCGGAAG
CAGCGGACCT
TCGACAACGG
CAGCATCCCC
CACCAGATCC
ACCTGGGAGA
GCTGCACGCC
ATTCTGCGGC
GGCAGGAAGA
TTTTTACCCA
TTCCTGAAGG
ACAACCGGGA
AAAGATCGAG
AAGATCCTGA
CCTTCCGCAT
CCCCTACTAC
GTGGGCCCTC
TGGCCAGGGG
AAACAGCAGA
TTCGCCTGGA
TGACCAGAAA
GAGCGAGGAA
ACCATCACCC
CCTGGAACTT
CGAGGAAGTG
GTGGACAAGG
GCGCTTCCGC
CCAGAGCTTC
ATCGAGCGGA
TGACCAACTT
CGATAAGAAC
CTGCCCAACG
AGAAGGTGCT
GCCCAAGCAC
AGCCTGCTGT
ACGAGTACTT
CACCGTGTAT
AACGAGCTGA
CCAAAGTGAA
ATACGTGACC
GAGGGAATGA
GAAAGCCCGC
CTTCCTGAGC
GGCGAGCAGA
AAAAGGCCAT
CGTGGACCTG
CTGTTCAAGA
CCAACCGGAA
AGTGACCGTG
AAGCAGCTGA
AAGAGGACTA
CTTCAAGAAA
ATCGAGTGCT
TCGACTCCGT
GGAAATCTCC
GGCGTGGAAG
ATCGGTTCAA
CGCCTCCCTG
GGCACATACC
ACGATCTGCT
GAAAATTATC
AAGGACAAGG
ACTTCCTGGA
CAATGAGGAA
AACGAGGACA
TTCTGGAAGA
TATCGTGCTG
ACCCTGACAC
TGTTTGAGGA
CAGAGAGATG
ATCGAGGAAC
GGCTGAAAAC
CTATGCCCAC
CTGTTCGACG
ACAAAGTGAT
GAAGCAGCTG
AAGCGGCGGA
GATACACCGG
CTGGGGCAGG
CTGAGCCGGA
AGCTGATCAA
CGGCATCCGG
GACAAGCAGT
CCGGCAAGAC
AATCCTGGAT
TTCCTGAAGT
CCGACGGCTT
CGCCAACAGA
AACTTCATGC
AGCTGATCCA
CGACGACAGC
CTGACCTTTA
AAGAGGACAT
CCAGAAAGCC
CAGGTGTCCG
GCCAGGGCGA
TAGCCTGCAC
GAGCACATTG
CCAATCTGGC
CGGCAGCCCC
GCCATTAAGA
AGGGCATCCT
GCAGACAGTG
AAGGTGGTGG
ACGAGCTCGT
GAAAGTGATG
GGCCGGCACA
AGCCCGAGAA
CATCGTGATC
GAAATGGCCA
GAGAGAACCA
GACCACCCAG
AAGGGACAGA
AGAACAGCCG
CGAGAGAATG
AAGCGGATCG
AAGAGGGCAT
CAAAGAGCTG
GGCAGCCAGA
TCCTGAAAGA
ACACCCCGTG
GAAAACACCC
AGCTGCAGAA
CGAGAAGCTG
TACCTGTACT
ACCTGCAGAA
TGGGCGGGAT
ATGTACGTGG
ACCAGGAACT
GGACATCAAC
CGGCTGTCCG
ACTACGATGT
GGACCATATC
GTGCCTCAGA
GCTTTCTGAA
GGACGACTCC
ATCGACAACA
AGGTGCTGAC
CAGAAGCGAC
AAGAACCGGG
GCAAGAGCGA
CAACGTGCCC
TCCGAAGAGG
TCGTGAAGAA
GATGAAGAAC
TACTGGCGGC
AGCTGCTGAA
CGCCAAGCTG
ATTACCCAGA
GAAAGTTCGA
CAATCTGACC
AAGGCCGAGA
GAGGCGGCCT
GAGCGAACTG
GATAAGGCCG
GCTTCATCAA
GAGACAGCTG
GTGGAAACCC
GGCAGATCAC
AAAGCACGTG
GCACAGATCC
TGGACTCCCG
GATGAACACT
AAGTACGACG
AGAATGACAA
GCTGATCCGG
GAAGTGAAAG
TGATCACCCT
GAAGTCCAAG
CTGGTGTCCG
ATTTCCGGAA
GGATTTCCAG
TTTTACAAAG
TGCGCGAGAT
CAACAACTAC
CACCACGCCC
ACGACGCCTA
CCTGAACGCC
GTCGTGGGAA
CCGCCCTGAT
CAAAAAGTAC
CCTAAGCTGG
AAAGCGAGTT
CGTGTACGGC
GACTACAAGG
TGTACGACGT
GCGGAAGATG
ATCGCCAAGA
GCGAGCAGGA
AATCGGCAAG
GCTACCGCCA
AGTACTTCTT
CTACAGCAAC
ATCATGAACT
TTTTCAAGAC
CGAGATTACC
CTGGCCAACG
GCGAGATCCG
GAAGCGGCCT
CTGATCGAGA
CAAACGGCGA
AACCGGGGAG
ATCGTGTGGG
ATAAGGGCCG
GGATTTTGCC
ACCGTGCGGA
AAGTGCTGAG
CATGCCCCAA
GTGAATATCG
TGAAAAAGAC
CGAGGTGCAG
ACAGGCGGCT
TCAGCAAAGA
GTCTATCCTG
CCCAAGAGGA
ACAGCGATAA
GCTGATCGCC
AGAAAGAAGG
ACTGGGACCC
TAAGAAGTAC
GGCGGCTTCG
ACAGCCCCAC
CGTGGCCTAT
TCTGTGCTGG
TGGTGGCCAA
AGTGGAAAAG
GGCAAGTCCA
AGAAACTGAA
GAGTGTGAAA
GAGCTGCTGG
GGATCACCAT
CATGGAAAGA
AGCAGCTTCG
AGAAGAATCC
CATCGACTTT
CTGGAAGCCA
AGGGCTACAA
AGAAGTGAAA
AAGGACCTGA
TCATCAAGCT
GCCTAAGTAC
TCCCTGTTCG
AGCTGGAAAA
CGGCCGGAAG
AGAATGCTGG
CCTCTGCCGG
CGAACTGCAG
AAGGGAAACG
AACTGGCCCT
GCCCTCCAAA
TATGTGAACT
TCCTGTACCT
GGCCAGCCAC
TATGAGAAGC
TGAAGGGCTC
CCCCGAGGAT
AATGAGCAGA
AACAGCTGTT
TGTGGAACAG
CACAAGCACT
ACCTGGACGA
GATCATCGAG
CAGATCAGCG
AGTTCTCCAA
GAGAGTGATC
CTGGCCGACG
CTAATCTGGA
CAAAGTGCTG
TCCGCCTACA
ACAAGCACCG
GGATAAGCCC
ATCAGAGAGC
AGGCCGAGAA
TATCATCCAC
CTGTTTACCC
TGACCAATCT
GGGAGCCCCT
GCCGCCTTCA
AGTACTTTGA
CACCACCATC
GACCGGAAGA
GGTACACCAG
CACCAAAGAG
GTGCTGGACG
CCACCCTGAT
CCACCAGAGC
ATCACCGGCC
TGTACGAGAC
ACGGATCGAC
CTGTCTCAGC
TGGGAGGCGA
CAAAAGGCCG
GCGGCCACGA
AAAAGGCCGG
CCAGGCAAAA
AAGAAAAAGT
AAAAGGGTGG
GATATGAAGA
TGAAGATGAA
ATATTTGGTG
TGTCAAATAA
AAAGCTTGTG
TGCTTAAGTT
TGTGTTTTTT
TCTTGGCTTG
TTGTGTTATG
AATTTGTGGC
TTTTTCTAAT
ATTAAATGAA
TGTAAGATCT
CATTATAATG
AATAAACAAA
TGTTTCTATA
ATCCATTGTG
AATGTTTTGT
TGGATCTCTT
CTGCAGCATA
TAACTACTGT
ATGTGCTATG
GTATGGACTA
TGGAATATGA
TTAAAGATAA
GGCGCGCCGA
CCCAGCTTTC
TTGTACAAAG
TGGTGATATC
ACTAGTGTAT
GTAGGTATAG
TAACATGAAC
AAGAACAGAC
TCAAGTACAA
GATCGCATAC
GAAAATGGAA
ATGGCAATGT
CACTTCCACA
TAATCAAACA
CGAATCCTCA
TATCAACAAG
GCCTGAGATT
CTAACTAGCT
CATAACAACT
TAGCCAATAG
TTACTTGAGA
CTACCAAATG
TATGTAGAAC
TAAAGACTAA
GGGACAGAGA
GTTCGTCTAA
ACAGGTGAAT
CTAGTCGTTG
TTATCTAATA
AACAATTCAG
CCCCAAATGC
AGAACACACA
TAGAGCTCTC
TATTGATTCA
AATTACGATC
TGATACTGAT
AACGTCTAGA
TTTTTAGGGT
TAAAGCAATC
AATCACCTGA
CGATTCAAGG
TGGTTGGATC
ATGACGATTC
CAGAAAACAT
CAAGCAAGCT
CTCAAAGCTA
CACTCTTTGG
GATCATACTG
AACTCTAACA
ACCTCGTTAT
GTCCCGTAGT
GCCAGTACAG
ACATCCTCGT
AACTCGGATT
GTGCACGATG
CCATGGCTAT
ACCCAACCTC
GGTCTTGGTC
ACACCAGGAA
CTCTCTGGTA
AGCTAGCTCC
ACTCCCCAGA
AACAACCGGC
GCCAAATTGC
GCGAATTGCT
GACCTGAAGA
CGGAACATCA
TCGTCGGGTC
CTTGGGCGAT
TGCGGCGGAA
GATGGGTCAG
CTTGGGCTTG
AGGACGAGAC
CCGAATCCGA
GTCTGTTGAA
AAGGTTGTTC
ATTGGGGATT
TGTATACGGA
GATTGGTCGT
CGAGAGGTTT
GAGGGAAAGG
ACAAATGGGT
TTGGCTCTGG
AGAAAGAGAG
TGCGGCTTTA
GAGAGAGAAT
TGAGAGGTTT
AGAGAGAGAT
GCGGCGGCGA
TGAGCGGAGG
AGAGACGACG
AGGACCTGCA
TTATCAAAGC
AGTGACGTGG
TGAAATTTGG
AACTTTTAAG
AGGCAGATAG
ATTTATTATT
TGTATCCATT
TTCTTCATTG
TTCTAGAATG
TCGCGGAACA
AATTTTAAAA
CTAAATCCTA
AATTTTTCTA
ATTTTGTTGC
CAATAGTGGA
TATGTGGGCC
GTATAGAAGG
AATCTATTGA
AGGCCCAAAC
CCATACTGAC
GAGCCCAAAG
GTTCGTTTTG
CGTTTTATGT
TTCGGTTCGA
TGCCAACGCC
ACATTCTGAG
CTAGGCAAAA
AACAAACGTG
TCTTTGAATA
GACTCCTCTC
GTTAACACAT
GCAGCGGCTG
CATGGTGACG
CCATTAACAC
GTGGCCTACA
ATTGCATGAT
GTCTCCATTG
ACACGTGACT
TCTCGTCTCC
TTTCTTAATA
TATCTAACAA
ACACTCCTAC
CTCTTCCAAA
ATATATACAC
ATCTTTTTGA
TCAATCTCTC
ATTCAAAATC
TCATTCTCTC
TAGTAAACAA
GAACAAAAAA
ATGGCGGATA
CAGCTAGAGG
AACCCATCAC
GATATCATCG
GCAGAGACCA
GTACCCGATG
ATGGGCCGAG
ACCGAGACCA
GTACCAGATG
TCCGGACGAG
GATCTGACTA
CTCCAAGTCT
AGGCAGATTG
CTAAAGCTGC
AACTGCTGTC
ACAGCTGGTG
GTTCCCTCCT
TGTTCTCTCC
AGCCTTACCC
TTGTTGGAAC
TGTCATAGCT
TTGACTGTTG
CAACACCTCT
GCTCGTTATC
TTCAGCCCAA
TCCTTGTCCC
GGCTCTCATC
ACAGTTGCAC
TCCTCATCAC
CGGTTTTCTT
TCCTCTGGAG
GGTTTGGCAT
TGCCGCTATA
ACCGTTTTCT
CTTGGATTTA
CAAGTAAGCA
CACATTTATC
ATCTTACTTC
ATAATTTTGT
GCAATATGTG
CATGCATGTG
TTGAGCCAGT
AGCTTTGGAT
CAATTTTTTT
GGTCGAATAA
CAAATGTAAC
AATAAGAAAT
TGCAAATTCT
AGGGAACATT
TGGTTAACTA
AATACGAAAT
TTGACCTAGC
TAGCTTGAAT
GTGTCTGTGT
ATATCATCTA
TATAGGTAAA
ATGCTTGGTA
TGATACCTAT
TGATTGTGAA
TAGGTACGCA
ACGGGAGAGC
ACCCACAGGG
ATCAGACAAG
TTGGACAGTG
CAAGGATGAA
GTTGGGAAGC
AAAGCTCAGG
ATCTGAAAGA
CAGAGCTCAG
TACTACGGAC
AGCAACATAC
TGGTGGGGAA
CATGACCGTG
ACCGTACTCG
TGGTGGCCAG
CACACTACTA
TGAGCGAGCT
GATTAAGGAG
AACATGCACA
TGAAGCTGTA
CATGGAGGGC
ACCGTGAACA
ACCACCACTT
CAAGTGCACA
TCCGAGGGCG
AAGGCAAGCC
CTACGAGGGC
ACCCAGACCA
TGAGAATCAA
GGTGGTCGAG
GGCGGCCCTC
TCCCCTTCGC
CTTCGACATC
CTGGCTACCA
GCTTCATGTA
CGGCAGCAGA
ACCTTCATCA
ACCACACCCA
GGGCATCCCC
GACTTCTTTA
AGCAGTCCTT
CCCTGAGGGC
TTCACATGGG
AGAGAGTCAC
CACATACGAA
GACGGGGGCG
TGCTGACCGC
TACCCAGGAC
ACCAGCCTCC
AGGACGGCTG
CCTCATCTAC
AACGTCAAGA
TCAGAGGGGT
GAACTTCCCA
TCCAACGGCC
CTGTGATGCA
GAAGAAAACA
CTCGGCTGGG
AGGCCAACAC
CGAGATGCTG
TACCCCGCTG
ACGGCGGCCT
GGAAGGCAGA
AGCGACATGG
CCCTGAAGCT
CGTGGGCGGG
GGCCACCTGA
TCTGCAACTT
CAAGACCACA
TACAGATCCA
AGAAACCCGC
TAAGAACCTC
AAGATGCCCG
GCGTCTACTA
TGTGGACCAC
AGACTGGAAA
GAATCAAGGA
GGCCGACAAA
GAGACCTACG
TCGAGCAGCA
CGAGGTGGCT
GTGGCCAGAT
ACTGCGACCT
CCCTAGCAAA
CTGGGGCACA
AGTGAATCGT
TCAAACATTT
GGCAATAAAG
TTTCTTAAGA
TTGAATCCTG
TTGCCGGTCT
TGCGATGATT
ATCATATAAT
TTCTGTTGAA
TTACGTTAAG
CATGTAATAA
TTAACATGTA
ATGCATGACG
TTATTTATGA
GATGGGTTTT
TATGATTAGA
GTCCCGCAAT
TATACATTTA
ATACGCGATA
GAAAACAAAA
TATAGCGCGC
AAACTAGGAT
AAATTATCGC
GCGCGGTGTC
ATCTATGTTA
CTAGATCACT
AGTGCGGCCG
CCTGCAGGTC
GACCATATGG
GAGAGCTCAA
GCTTAGCTTG
AGCTTGGATC
AGATTGTCGT
TTCCCGCCTT
CAGTTTAAAC
TATCAGTGTT
TGACAGGATA
TATTGGCGGG
TAAACCTAAG
AGAAAAGAGC
GTTTATTAGA
ATAATCGGAT
ATTTAAAAGG
GCGTGAAAAG
GTTTATCCGT
TCGTCCATTT
GTATGTGCAT
GCCAACCACA
GGGTTCCCCT
CGGGATCAAA
GTACTTTAAA
GTACTTTAAA
GTACTTTAAA
GTACTTTGAT
CCAACCCCTC
CGCTGCTATA
GTGCAGTCGG
CTTCTGACGT
TCAGTGCAGC
CGTCTTCTGA
AAACGACATG
TCGCACAAGT
CCTAAGTTAC
GCGACAGGCT
GCCGCCCTGC
CCTTTTCCTG
GCGTTTTCTT
GTCGCGTGTT
TTAGTCGCAT
AAAGTAGAAT
ACTTGCGACT
AGAACCGGAG
ACATTACGCC
ATGAACAAGA
GCGCCGCCGC
TGGCCTGCTG
GGCTATGCCC
GCGTCAGCAC
CGACGACCAG
GACTTGACCA
ACCAACGGGC
CGAACTGCAC
GCGGCCGGCT
GCACCAAGCT
GTTTTCCGAG
AAGATCACCG
GCACCAGGCG
CGACCGCCCG
GAGCTGGCCA
GGATGCTTGA
CCACCTACGC
CCTGGCGACG
TTGTGACAGT
GACCAGGCTA
GACCGCCTGG
CCCGCAGCAC
CCGCGACCTA
CTGGACATTG
CCGAGCGCAT
CCAGGAGGCC
GGCGCGGGCC
TGCGTAGCCT
GGCAGAGCCG
TGGGCCGACA
CCACCACGCC
GGCCGGCCGC
ATGGTGTTGA
CCGTGTTCGC
CGGCATTGCC
GAGTTCGAGC
GTTCCCTAAT
CATCGACCGC
ACCCGGAGCG
GGCGCGAGGC
CGCCAAGGCC
CGAGGCGTGA
AGTTTGGCCC
CCGCCCTACC
CTCACCCCGG
CACAGATCGC
GCACGCCCGC
GAGCTGATCG
ACCAGGAAGG
CCGCACCGTG
AAAGAGGCGG
CTGCACTGCT
TGGCGTGCAT
CGCTCGACCC
TGTACCGCGC
ACTTGAGCGC
AGCGAGGAAG
TGACGCCCAC
CGAGGCCAGG
CGGCGCGGTG
CCTTCCGTGA
GGACGCATTG
ACCGAGGCCG
ACGCCCTGGC
GGCCGCCGAG
AATGAACGCC
AAGAGGAACA
AGCATGAAAC
CGCACCAGGA
CGGCCAGGAC
GAACCGTTTT
TCATTACCGA
AGAGATCGAG
GCGGAGATGA
TCGCGGCCGG
GTACGTGTTC
GAGCCGCCCG
CGCACGTCTC
AACCGTGCGG
CTGCATGAAA
TCCTGGCCGG
TTTGTCTGAT
GCCAAGCTGG
CGGCCTGGCC
GGCCAGCTTG
GCCGCTGAAG
AAACCGAGCG
CCGCCGTCTA
AAAAGGTGAT
GTGTATTTGA
GTAAAACAGC
TTGCGTCATG
CGGTCGCTGC
GTATATGATG
CGATGAGTAA
ATAAACAAAT
ACGCAAGGGG
AACGCATGAA
GGTTATCGCT
GTACTTAACC
AGAAAGGCGG
GTCAGGCAAG
ACGACCATCG
CAACCCATCT
AGCCCGCGCC
CTGCAACTCG
CCGGGGCCGA
TGTTCTGTTA
GTCGATTCCG
ATCCCCAGGG
CAGTGCCCGC
GATTGGGCGG
CCGTGCGGGA
AGATCAACCG
CTAACCGTTG
TCGGCATCGA
CCGCCCGACG
ATTGACCGCG
ACGTGAAGGC
CATCGGCCGG
CGCGACTTCG
TAGTGATCGA
CGGAGCGCCC
CAGGCGGCGG
ACTTGGCTGT
GTCCGCGATC
AAGGCAGCCG
ACTTCGTGCT
GATTCCGGTG
CAGCCAAGCC
CTTACGACAT
ATGGGCCACC
GCCGACCTGG
TGGAGCTGGT
TAAGCAGCGC
ATTGAGGTCA
CGGATGGAAG
GCTACAAGCG
GCCTTTGTCG
TGTCGCGGGC
GATCAAAGGC
ACGCGCATCG
GCGGTGAGGT
TGCCGAGGCG
CTGGCCGGGT
ACGAGCTGCC
CATTCTTGAG
TCCCGTATCA
CGCAGCGCGT
GAGCTACCCA
GGCACTGCCG
CCGCCGGCAC
AACCGTTCTT
GAATCAGAAC
CCGAGGGCGA
CGCTGCCCGC
GAGGTCCAGG
CGCTGGCCGC
TGAAATTAAA
TCAAAACTCA
TTTGAGTTAA
TGAGGTAAAG
AGAAAATGAG
CAAAAGCACA
AACACGCTAA
GTGCCGGCCG
TCCGAGCGCA
CGCAGCAGCA
AGGCTGCAAC
GTTGGCCAGC
CTGGCAGACA
CGCCAGCCAT
GAAGCGGGTC
AACTTTCAGT
TGCCGGCGGA
GGATCACACC
AAGCTGAAGA
TGTACGCGGT
ACGCCAAGGC
AAGACCATTA
CCGAGCTGCT
ATCTGAATAC
ATCGCGCAGC
TACCAGAGTA
AATGAGCAAA
TGAATAAATG
AGTAGATGAA
TTTTAGCGGC
TAAAGGAGGC
GGCATGGAAA
ATCAAGAACA
ACCAGGCACC
GACGCCGTGG
AATGCCCCAT
GTGTGGAGGA
ACGGGCGGTT
GGCCAGGCGT
AAGCGGCTGG
GTTGTCTGCC
GGCCCTGCAA
TGGCACTGGA
ACCCCCAAGC
CCGAGGAATC
GGCGTGAGCG
GTCGCAAACC
ATCCGGCCCG
GTACAAATCG
GCGCGGCGCT
GGGTGATGAC
CTGGTGGAGA
AGTTGAAGGC
CGCGCAGGCC
GCCCAGCGGC
AACGCATCGA
GGCAGAAGCA
CGCCCCGGTG
AATCGTGGCA
AGCGGCCGCT
GATCGAATCC
GCAAAGAATC
CCGGCAACCG
CCGGCAGCCG
GTGCGCCGTC
GATTAGGAAG
CCGCCCAAGG
GCGACGAGCA
ACCAGATTTT
TTCGTTCCGA
TGCTCTATGA
CGTGGGCACC
CGCGATAGTC
GCAGCATCAT
GGACGTGGCC
GTTTTCCGTC
TGTCGAAGCG
TGACCGACGA
GCTGGCGAGG
TGATCCGCTA
CGAGCTTCCA
GACGGGCACG
TAGAGGTTTC
CGCAGGGCCG
GCCGGCATGG
CCAGTGTGTG
GGATTACGAC
CTGGTACTGA
TGGCGGTTTC
CCATCTAACC
GAATCCATGA
ACCGATACCG
GGAAGGGAAG
GGAGACAAGC
CCGGCCGCGT
GTTCCGTCCA
CACGTTGCGG
ACGTACTCAA
GTTCTGCCGG
CGAGCCGATG
GCGGAAAGCA
GAAAGACGAC
CTGGTAGAAA
CCTGCATTCG
GTTAAACACC
ACGCACGTTG
CCATGCAGCG
TACGAAGAAG
GCCAAGAACG
GCCGCCTGGT
GACGGTATCC
GAGGGTGAAG
CCTTGATTAG
CCGCTACAAG
ATCGTAAAGA
GCGAAACCGG
GCGGCCGGAG
TACATCGAGA
TCGAGCTAGC
TGATTGGATG
TACCGCGAGA
TCACAGAAGG
CAAGAACCCG
GACGTGCTGA
CGGTTCACCC
CGATTACTTT
TTGATCGATC
CCGGCATCGG
CCGTTTTCTC
TACCGCCTGG
CACGCCGCGC
CGCAGGCAAG
GCAGAAGCCA
GATGGTTGTT
CAAGACGATC
TACGAACGCA
GTGGCAGCGC
CGGAGAGTTC
AAGAAGTTCT
GTTTCACCGT
GCGCAAGCTG
ATCGGGTCAA
ATGACCTGCC
GGAGTACGAT
TTGAAGGAGG
AGGCGGGGCA
GGCTGGCCCG
ATCCTAGTCA
TGCGCTACCG
CAACCTGATC
GAGGGCGAAG
CATCCGCCGG
TTCCTAATGT
ACGGAGCAGA
TGCTAGGGCA
AATTGCCCTA
GCAGGGGAAA
AAGGTCGAAA
AGGTCTCTTT
CCTGTGGATA
GCACGTACAT
TGGGAACCCA
AAGCCGTACA
TTGGGAACCG
GAACCCGTAC
ATTGGGAACC
CAAAGCCGTA
CATTGGGAAC
CGGTCACACA
TGTAAGTGAC
TGATATAAAA
GAGAAAAAAG
GCGATTTTTC
CGCCTAAAAC
TCTTTAAAAC
TTATTAAAAC
TCTTAAAACC
CGCCTGGCCT
GTGCATAACT
GTCTGGCCAG
CGCACAGCCG
AAGAGCTGCA
AAAAGCGCCT
ACCCTTCGGT
CGCTGCGCTC
CCTACGCCCC
GCCGCTTCGC
GTCGGCCTAT
CGCGGCCGCT
GGCCGCTCAA
AAATGGCTGG
CCTACGGCCA
GGCAATCTAC
CAGGGCGCGG
ACAAGCCGCG
CCGTCGCCAC
TCGACCGCCG
GCGCCCACAT
CAAGGCACCC
TGCCTCGCGC
GTTTCGGTGA
TGACGGTGAA
AACCTCTGAC
ACATGCAGCT
CCCGGAGACG
GTCACAGCTT
GTCTGTAAGC
GGATGCCGGG
AGCAGACAAG
CCCGTCAGGG
CGCGTCAGCG
GGTGTTGGCG
GGTGTCGGGG
CGCAGCCATG
ACCCAGTCAC
GTAGCGATAG
CGGAGTGTAT
ACTGGCTTAA
CTATGCGGCA
TCAGAGCAGA
TTGTACTGAG
AGTGCACCAT
ATGCGGTGTG
AAATACCGCA
CAGATGCGTA
AGGAGAAAAT
ACCGCATCAG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGGAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
CATGATATAT
CTCCCAATTT
GTGTAGGGCT
TATTATGCAC
GCTTAAAAAT
AATAAAAGCA
GACTTGACCT
GATAGTTTGG
CTGTGAGCAA
TTATGTGCTT
AGTGCATCTA
ACGCTTGAGT
TAAGCCGCGC
CGCGAAGCGG
CGTCGGCTTG
AACGAATTTC
TAGCTAGACA
TTATTTGCCG
ACTACCTTGG
TGATCTCGCC
TTTCACGTAG
TGGACAAATT
CTTCCAACTG
ATCTGCGCGC
GAGGCCAAGC
GATCTTCTTC
TTGTCCAAGA
TAAGCCTGTC
TAGCTTCAAG
TATGACGGGC
TGATACTGGG
CCGGCAGGCG
CTCCATTGCC
CAGTCGGCAG
CGACATCCTT
CGGCGCGATT
TTGCCGGTTA
CTGCGCTGTA
CCAAATGCGG
GACAACGTAA
GCACTACATT
TCGCTCATCG
CCAGCCCAGT
CGGGCGGCGA
GTTCCATAGC
GTTAAGGTTT
CATTTAGCGC
CTCAAATAGA
TCCTGTTCAG
GAACCGGATC
AAAGAGTTCC
TCCGCCGCTG
GACCTACCAA
GGCAACGCTA
TGTTCTCTTG
CTTTTGTCAG
CAAGATAGCC
AGATCAATGT
CGATCGTGGC
TGGCTCGAAG
ATACCTGCAA
GAATGTCATT
GCGCTGCCAT
TCTCCAAATT
GCAGTTCGCG
CTTAGCTGGA
TAACGCCACG
GAATGATGTC
GTCGTGCACA
ACAATGGTGA
CTTCTACAGC
GCGGAGAATC
TCGCTCTCTC
CAGGGGAAGC
CGAAGTTTCC
AAAAGGTCGT
TGATCAAAGC
TCGCCGCGTT
GTTTCATCAA
GCCTTACGGT
CACCGTAACC
AGCAAATCAA
TATCACTGTG
TGGCTTCAGG
CCGCCATCCA
CTGCGGAGCC
GTACAAATGT
ACGGCCAGCA
ACGTCGGTTC
GAGATGGCGC
TCGATGACGC
CAACTACCTC
TGATAGTTGA
GTCGATACTT
CGGCGATCAC
CGCTTCCCCC
ATGATGTTTA
ACTTTGTTTT
AGGGCGACTG
CCCTGCTGCG
TAACATCGTT
GCTGCTCCAT
AACATCAAAC
ATCGACCCAC
GGCGTAACGC
GCTTGCTGCT
TGGATGCCCG
AGGCATAGAC
TGTACCCCAA
AAAAACAGTC
ATAACAAGCC
ATGAAAACCG
CCACTGCGCC
GTTACCACCG
CTGCGTTCGG
TCAAGGTTCT
GGACCAGTTG
CGTGAGCGCA
TACGCTACTT
GCATTACAGC
TTACGAACCG
AACAGGCTTA
TGTCCACTGG
GTTCGTGCCC
GAATTGATCA
CAGGCAGCAA
CGCTCTGTCA
TCGTTACAAT
CAACATGCTA
CCCTCCGCGA
GATCATCCGT
GTTTCAAACC
CGGCAGCTTA
GTTGCCGTTC
TTCCGAATAG
CATCGGTAAC
ATGAGCAAAG
TCTGCCGCCT
TACAACGGCT
CTCCCGCTGA
CGCCGTCCCG
GACTGATGGG
CTGCCTGTAT
CGAGTGGTGA
TTTTGTGCCG
AGCTGCCGGT
CGGGGAGCTG
TTGGCTGGCT
GGTGGCAGGA
TATATTGTGG
TGTAAACAAA
TTGACGCTTA
GACAACTTAA
TAACACATTG
CGGACGTTTT
TAATGTACTG
AATTAACGCC
GAATTGAATT
ATCAGCTTGC
ATGCCGGTCG
ATCTAGTAAC
ATAGATGACA
CCGCGCGCGA
TAATTTATCC
TAGTTTGCGC
GCTATATTTT
GTTTTCTATC
GCGTATTAAA
TGTATAATTG
CGGGACTCTA
ATCATAAAAA
CCCATCTCAT
AAATAACGTC
ATGCATTACA
TGTTAATTAT
TACATGCTTA
ACGTAATTCA
ACAGAAATTA
TATGATAATC
ATCGCAAGAC
CGGCAACAGG
ATTCAATCTT
AAGAAACTTT
ATTGCCAAAT
GTTTGAACGA
TCTGCTTGAC
TCTAGGGAAT
TAATTCCTGA
ATCACTGCGA
CCGGCCCTCC
CGCGACCCAG
CCGAGCGAGC
TTAGCGAACT
GTGGACGAGA
ACTGTGCCAC
CAAGCGTAAG
GCCGTTCTCT
CGCATTTGCC
TTGCTAGGCT
CGCGCGAGTT
GCTGGCTGAG
GCGTTCTCGA
AATCAGCTCT
TGTTCGGTCG
GCATCTACTC
TATTCCTTTG
CCCTCGGACG
AGTGCTGGGG
CGTCGGTTTC
CACTATCGGC
GAGTACTTCT
ACACAGCCAT
CGGTCCAGAC
GGCCGCGCTT
CTGCGGGCGA
TTTGTGTACG
CCCGACAGTC
CCGGCTCCGG
ATCGGACGAT
TGCGTCGCAT
CGACCCTGCG
CCCAAGCTGC
ATCATCGAAA
TTGCCGTCAA
CCAAGCTCTG
ATAGAGTTGG
TCAAGACCAA
TGCGGAGCAT
ATACGCCCGG
AGCCTTGGCG
ATCCTGCAAG
CTCCGGATGC
CTCCGCTCGA
AGTAGCGCGT
CTGCTGCTCC
ATACAAGCCA
ACCACGGCCT
CCAGAAGAAG
ATGTTGGCGA
CCTCGTATTG
GGAATCCCCG
AACATCGCCT
CGCTCCAGTC
AATGACCGCT
GTTATGCGGC
CATTGTCCGT
CAGGACATTG
TTGGAGCCGA
AATCCGCGTG
CACGAGGTGC
CGGACTTCGG
GGCAGTCCTC
GGCCCAAAGC
ATCAGCTCAT
CGAGAGCCTG
CGCGACGGAC
GCACTGACGG
TGTCGTCCAT
CACAGTTTGC
CAGTGATACA
CATGGGGATC
AGCAATCGCG
CATATGAAAT
CACGCCATGT
AGTGTATTGA
CCGATTCCTT
GCGGTCCGAA
TGGGCCGAAC
CCGCTCGTCT
GGCTAAGATC
GGCCGCAGCG
ATAGCATCCA
TAGCCTCCGC
GACCGGCTGA
AGAACAGCGG
GCAGTTCGGT
TTCAGGCAGG
TCTTGCAACG
TGACACCCTG
TGCACGGCGG
GAGATGCAAT
AGGTCAGGCT
CTCGCTGAAC
TCCCCAATGT
CAAGCACTTC
CGGAATCGGG
AGCGCGGCCG
ATGCAAAGTG
CCGATAAACA
TAACGATCTT
TGTAGAAACC
ATCGGCGCAG
CTATTTACCC
GCAGGACATA
TCCACGCCCT
CCTACATCGA
AGCTGAAAGC
ACGAGATTCT
TCGCCCTCCG
AGAGCTGCAT
CAGGTCGGAG
ACGCTGTCGA
ACTTTTCGAT
CAGAAACTTC
TCGACAGACG
TCGCGGTGAG
TTCAGGCTTT
TTCATAGGGG
GGATTCGAGT
TGAGAGTGAA
TATGAGACTC
TAATTGGATA
CCGAGGGGAA
TTTATGGAAC
GTCAGTGGAG
CATTTTTGAC
AAGAAATATT
TGCTAGCTGA
TAGTGACCTT
AGGCGACTTT
TGAACGCGCA
ATAATGGTTT
CTGACGTATG
TGCTTAGCTC
ATTAAACTCC
AGAAACCCGC
GGCTCAGTGG
CTCCTTCAAC
GTTGCGGTTC
TGTCAGTTCC
AAACGTAAAA
CGGCTTGTCC
CGCGTCATCG
GCGGGGGTCA
TAACGTGACT
CCCTTAATTC
TCCGCTCATG
ATCGATAATT
CGCGGTACCC
GGGGATCCTC
TAGAGGGCC