名称: | pBUN6I11-AtU6-ALKBH10B-sgRNA1-35S-Cas9 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | Bar |
荧光标记: | |
启动子: | UBI,OsU3 |
复制子: | pSa |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTTTACCCGC
CAATATATCC
TGTCAAACAC
TGATAGTTTA
AACTGAAGGC
GGGAAACGAC
AATCTGATCC
AAGCTCAAGC
TAAGCTCACG
TGACGGAATT
AAGCTTCGTT
GAACAACGGA
AACTCGACTT
GCCTTCCGCA
CAATACATCA
TTTCTTCTTA
GCTTTTTTTC
TTCTTCTTCG
TTCATACAGT
TTTTTTTTGT
TTATCAGCTT
ACATTTTCTT
GAACCGTAGC
TTTCGTTTTC
TTCTTTTTAA
CTTTCCATTC
GGAGTTTTTG
TATCTTGTTT
CATAGTTTGT
CCCAGGATTA
GAATGATTAG
GCATCGAACC
TTCAAGAATT
TGATTGAATA
AAACATCTTC
ATTCTTAAGA
TATGAAGATA
ATCTTCAAAA
GGCCCCTGGG
AATCTGAAAG
AAGAGAAGCA
GGCCCATTTA
TATGGGAAAG
AACAATAGTA
TTTCTTATAT
AGGCCCATTT
AAGTTGAAAA
CAATCTTCAA
AAGTCCCACA
TCGCTTAGAT
AAGAAAACGA
AGCTGAGTTT
ATATACAGCT
AGAGTCGAAG
TAGTGATTGG
CACTCTCTTG
TAGCTGACGG
TTTTAGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTATCAACT
TGAAAAAGTG
GCACCGAGTC
GGTGCTTTTT
TTGTCCCTTC
GAAGGGCAAT
TCTGCAGATA
TCCATCACAC
TGGCGGCCGC
TCGAGGTCGA
CGGTATCGAT
AAGCTTGATA
TCGAATTCCT
GCAGTGCAGC
GTGACCCGGT
CGTGCCCCTC
TCTAGAGATA
ATGAGCATTG
CATGTCTAAG
TTATAAAAAA
TTACCACATA
TTTTTTTTGT
CACACTTGTT
TGAAGTGCAG
TTTATCTATC
TTTATACATA
TATTTAAACT
TTACTCTACG
AATAATATAA
TCTATAGTAC
TACAATAATA
TCAGTGTTTT
AGAGAATCAT
ATAAATGAAC
AGTTAGACAT
GGTCTAAAGG
ACAATTGAGT
ATTTTGACAA
CAGGACTCTA
CAGTTTTATC
TTTTTAGTGT
GCATGTGTTC
TCCTTTTTTT
TTGCAAATAG
CTTCACCTAT
ATAATACTTC
ATCCATTTTA
TTAGTACATC
CATTTAGGGT
TTAGGGTTAA
TGGTTTTTAT
AGACTAATTT
TTTTAGTACA
TCTATTTTAT
TCTATTTTAG
CCTCTAAATT
AAGAAAACTA
AAACTCTATT
TTAGTTTTTT
TATTTAATAA
TTTAGATATA
AAATAGAATA
AAATAAAGTG
ACTAAAAATT
AAACAAATAC
CCTTTAAGAA
ATTAAAAAAA
CTAAGGAAAC
ATTTTTCTTG
TTTCGAGTAG
ATAATGCCAG
CCTGTTAAAC
GCCGTCGACG
AGTCTAACGG
ACACCAACCA
GCGAACCAGC
AGCGTCGCGT
CGGGCCAAGC
GAAGCAGACG
GCACGGCATC
TCTGTCGCTG
CCTCTGGACC
CCTCTCGAGA
GTTCCGCTCC
ACCGTTGGAC
TTGCTCCGCT
GTCGGCATCC
AGAAATGCGT
GGCGGAGCGG
CAGACGTGAG
CCGGCACGGC
AGGCGGCCTC
CTCCTCCTCT
CACGGCACGG
CAGCTACGGG
GGATTCCTTT
CCCACCGCTC
CTTCGCTTTC
CCTTCCTCGC
CCGCCGTAAT
AAATAGACAC
CCCCTCCACA
CCCTCTTTCC
CCAACCTCGT
GTTGTTCGGA
GCGCACACAC
ACACAACCAG
ATCTCCCCCA
AATCCACCCG
TCGGCACCTC
CGCTTCAAGG
TACGCCGCTC
GTCCTCCCCC
CCCCCCCCTC
TCTACCTTCT
CTAGATCGGC
GTTCCGGTCC
ATGGTTAGGG
CCCGGTAGTT
CTACTTCTGT
TCATGTTTGT
GTTAGATCCG
TGTTTGTGTT
AGATCCGTGC
TGCTAGCGTT
CGTACACGGA
TGCGACCTGT
ACGTCAGACA
CGTTCTGATT
GCTAACTTGC
CAGTGTTTCT
CTTTGGGGAA
TCCTGGGATG
GCTCTAGCCG
TTCCGCAGAC
GGGATCGATT
TCATGATTTT
TTTTGTTTCG
TTGCATAGGG
TTTGGTTTGC
CCTTTTCCTT
TATTTCAATA
TATGCCGTGC
ACTTGTTTGT
CGGGTCATCT
TTTCATGCTT
TTTTTTGTCT
TGGTTGTGAT
GATGTGGTCT
GGTTGGGCGG
TCGTTCTAGA
TCGGAGTAGA
ATTCTGTTTC
AAACTACCTG
GTGGATTTAT
TAATTTTGGA
TCTGTATGTG
TGTGCCATAC
ATATTCATAG
TTACGAATTG
AAGATGATGG
ATGGAAATAT
CGATCTAGGA
TAGGTATACA
TGTTGATGCG
GGTTTTACTG
ATGCATATAC
AGAGATGCTT
TTTGTTCGCT
TGGTTGTGAT
GATGTGGTGT
GGTTGGGCGG
TCGTTCATTC
GTTCTAGATC
GGAGTAGAAT
ACTGTTTCAA
ACTACCTGGT
GTATTTATTA
ATTTTGGAAC
TGTATGTGTG
TGTCATACAT
CTTCATAGTT
ACGAGTTTAA
GATGGATGGA
AATATCGATC
TAGGATAGGT
ATACATGTTG
ATGTGGGTTT
TACTGATGCA
TATACATGAT
GGCATATGCA
GCATCTATTC
ATATGCTCTA
ACCTTGAGTA
CCTATCTATT
ATAATAAACA
AGTATGTTTT
ATAATTATTT
TGATCTTGAT
ATACTTGGAT
GATGGCATAT
GCAGCAGCTA
TATGTGGATT
TTTTTAGCCC
TGCCTTCATA
CGCTATTTAT
TTGCTTGGTA
CTGTTTCTTT
TGTCGATGCT
CACCCTGTTG
TTTGGTGTTA
CTTCTGCAGC
CCGGGGGATC
CCCAATACTT
GTATGGCCGC
GGCCGCTCTA
GATGGATTAC
AAGGACCACG
ACGGGGATTA
CAAGGACCAC
GACATTGATT
ACAAGGATGA
TGATGACAAG
ATGGCTCCGA
AGAAGAAGAG
GAAGGTTGGC
ATCCACGGGG
TGCCAGCTGC
TGACAAGAAG
TACTCGATCG
GCCTCGCCAT
TGGGACTAAC
TCTGTTGGCT
GGGCCGTGAT
CACCGACGAG
TACAAGGTGC
CCTCAAAGAA
GTTCAAGGTC
CTGGGCAACA
CCGATCGGCA
TTCCATCAAG
AAGAATCTCA
TTGGCGCTCT
CCTGTTCGAC
AGCGGCGAGA
CGGCTGAGGC
TACGCGGCTC
AAGCGCACCG
CCCGCAGGCG
GTACACGCGC
AGGAAGAATC
GCATCTGCTA
CCTGCAGGAG
ATTTTCTCCA
ACGAGATGGC
GAAGGTTGAC
GATTCTTTCT
TCCACAGGCT
GGAGGAGTCA
TTCCTCGTGG
AGGAGGATAA
GAAGCACGAG
CGGCATCCAA
TCTTCGGCAA
CATTGTCGAC
GAGGTTGCCT
ACCACGAGAA
GTACCCTACG
ATCTACCATC
TGCGGAAGAA
GCTCGTGGAC
TCCACAGATA
AGGCGGACCT
CCGCCTGATC
TACCTCGCTC
TGGCCCACAT
GATTAAGTTC
AGGGGCCATT
TCCTGATCGA
GGGGGATCTC
AACCCGGACA
ATAGCGATGT
TGACAAGCTG
TTCATCCAGC
TCGTGCAGAC
GTACAACCAG
CTCTTCGAGG
AGAACCCCAT
TAATGCGTCA
GGCGTCGACG
CGAAGGCTAT
CCTGTCCGCT
AGGCTCTCGA
AGTCTCGGCG
CCTCGAGAAC
CTGATCGCCC
AGCTGCCGGG
CGAGAAGAAG
AACGGCCTGT
TCGGGAATCT
CATTGCGCTC
AGCCTGGGGC
TCACGCCCAA
CTTCAAGTCG
AATTTCGATC
TCGCTGAGGA
CGCCAAGCTG
CAGCTCTCCA
AGGACACATA
CGACGATGAC
CTGGATAACC
TCCTGGCCCA
GATCGGCGAT
CAGTACGCGG
ACCTGTTCCT
CGCTGCCAAG
AATCTGTCGG
ACGCCATCCT
CCTGTCTGAT
ATTCTCAGGG
TGAACACCGA
GATTACGAAG
GCTCCGCTCT
CAGCCTCCAT
GATCAAGCGC
TACGACGAGC
ACCATCAGGA
TCTGACCCTC
CTGAAGGCGC
TGGTCAGGCA
GCAGCTCCCC
GAGAAGTACA
AGGAGATCTT
CTTCGATCAG
TCGAAGAACG
GCTACGCTGG
GTACATTGAC
GGCGGGGCCT
CTCAGGAGGA
GTTCTACAAG
TTCATCAAGC
CGATTCTGGA
GAAGATGGAC
GGCACGGAGG
AGCTGCTGGT
GAAGCTCAAT
CGCGAGGACC
TCCTGAGGAA
GCAGCGGACA
TTCGATAACG
GCAGCATCCC
ACACCAGATT
CATCTCGGGG
AGCTGCACGC
TATCCTGAGG
AGGCAGGAGG
ACTTCTACCC
TTTCCTCAAG
GATAACCGCG
AGAAGATCGA
GAAGATTCTG
ACTTTCAGGA
TCCCGTACTA
CGTCGGCCCA
CTCGCTAGGG
GCAACTCCCG
CTTCGCTTGG
ATGACCCGCA
AGTCAGAGGA
GACGATCACG
CCGTGGAACT
TCGAGGAGGT
GGTCGACAAG
GGCGCTAGCG
CTCAGTCGTT
CATCGAGAGG
ATGACGAATT
TCGACAAGAA
CCTGCCAAAT
GAGAAGGTGC
TCCCTAAGCA
CTCGCTCCTG
TACGAGTACT
TCACAGTCTA
CAACGAGCTG
ACTAAGGTGA
AGTATGTGAC
CGAGGGCATG
AGGAAGCCGG
CTTTCCTGTC
TGGGGAGCAG
AAGAAGGCCA
TCGTGGACCT
CCTGTTCAAG
ACCAACCGGA
AGGTCACGGT
TAAGCAGCTC
AAGGAGGACT
ACTTCAAGAA
GATTGAGTGC
TTCGATTCGG
TCGAGATCTC
TGGCGTTGAG
GACCGCTTCA
ACGCCTCCCT
GGGGACCTAC
CACGATCTCC
TGAAGATCAT
TAAGGATAAG
GACTTCCTGG
ACAACGAGGA
GAATGAGGAT
ATCCTCGAGG
ACATTGTGCT
GACACTCACT
CTGTTCGAGG
ACCGGGAGAT
GATCGAGGAG
CGCCTGAAGA
CTTACGCCCA
TCTCTTCGAT
GACAAGGTCA
TGAAGCAGCT
CAAGAGGAGG
AGGTACACCG
GCTGGGGGAG
GCTGAGCAGG
AAGCTCATCA
ACGGCATTCG
GGACAAGCAG
TCCGGGAAGA
CGATCCTCGA
CTTCCTGAAG
AGCGATGGCT
TCGCGAACCG
CAATTTCATG
CAGCTGATTC
ACGATGACAG
CCTCACATTC
AAGGAGGATA
TCCAGAAGGC
TCAGGTGAGC
GGCCAGGGGG
ACTCGCTGCA
CGAGCATATC
GCGAACCTCG
CTGGCTCGCC
AGCTATCAAG
AAGGGGATTC
TGCAGACCGT
GAAGGTTGTG
GACGAGCTGG
TGAAGGTCAT
GGGCAGGCAC
AAGCCTGAGA
ACATCGTCAT
TGAGATGGCC
CGGGAGAATC
AGACCACGCA
GAAGGGCCAG
AAGAACTCAC
GCGAGAGGAT
GAAGAGGATC
GAGGAGGGCA
TTAAGGAGCT
GGGGTCCCAG
ATCCTCAAGG
AGCACCCGGT
GGAGAACACG
CAGCTGCAGA
ATGAGAAGCT
CTACCTGTAC
TACCTCCAGA
ATGGCCGCGA
TATGTATGTG
GACCAGGAGC
TGGATATTAA
CAGGCTCAGC
GATTACGACG
TCGATGCCAT
CGTTCCACAG
TCATTCCTGA
AGGATGACTC
CATTGACAAC
AAGGTCCTCA
CCAGGTCGGA
CAAGAACCGG
GGCAAGTCTG
ATAATGTTCC
TTCAGAGGAG
GTCGTTAAGA
AGATGAAGAA
CTACTGGCGC
CAGCTCCTGA
ATGCCAAGCT
GATCACGCAG
CGGAAGTTCG
ATAACCTCAC
AAAGGCTGAG
AGGGGCGGGC
TCTCTGAGCT
GGACAAGGCG
GGCTTCATCA
AGAGGCAGCT
GGTCGAGACA
CGGCAGATCA
CTAAGCACGT
TGCGCAGATT
CTCGACTCAC
GGATGAACAC
TAAGTACGAT
GAGAATGACA
AGCTGATCCG
CGAGGTGAAG
GTCATCACCC
TGAAGTCAAA
GCTCGTCTCC
GACTTCAGGA
AGGATTTCCA
GTTCTACAAG
GTTCGGGAGA
TCAACAATTA
CCACCATGCC
CATGACGCGT
ACCTGAACGC
GGTGGTCGGC
ACAGCTCTGA
TCAAGAAGTA
CCCAAAGCTC
GAGAGCGAGT
TCGTGTACGG
GGACTACAAG
GTTTACGATG
TGAGGAAGAT
GATCGCCAAG
TCGGAGCAGG
AGATTGGCAA
GGCTACCGCC
AAGTACTTCT
TCTACTCTAA
CATTATGAAT
TTCTTCAAGA
CAGAGATCAC
TCTGGCCAAT
GGCGAGATCC
GGAAGCGCCC
CCTCATCGAG
ACGAACGGCG
AGACGGGGGA
GATCGTGTGG
GACAAGGGCA
GGGATTTCGC
GACCGTCAGG
AAGGTTCTCT
CCATGCCACA
AGTGAATATC
GTCAAGAAGA
CAGAGGTCCA
GACTGGCGGG
TTCTCTAAGG
AGTCAATTCT
GCCTAAGCGG
AACAGCGACA
AGCTCATCGC
CCGCAAGAAG
GACTGGGATC
CGAAGAAGTA
CGGCGGGTTC
GACAGCCCCA
CTGTGGCCTA
CTCGGTCCTG
GTTGTGGCGA
AGGTTGAGAA
GGGCAAGTCC
AAGAAGCTCA
AGAGCGTGAA
GGAGCTGCTG
GGGATCACGA
TTATGGAGCG
CTCCAGCTTC
GAGAAGAACC
CGATCGATTT
CCTGGAGGCG
AAGGGCTACA
AGGAGGTGAA
GAAGGACCTG
ATCATTAAGC
TCCCCAAGTA
CTCACTCTTC
GAGCTGGAGA
ACGGCAGGAA
GCGGATGCTG
GCTTCCGCTG
GCGAGCTGCA
GAAGGGGAAC
GAGCTGGCTC
TGCCGTCCAA
GTATGTGAAC
TTCCTCTACC
TGGCCTCCCA
CTACGAGAAG
CTCAAGGGCA
GCCCCGAGGA
CAACGAGCAG
AAGCAGCTGT
TCGTCGAGCA
GCACAAGCAT
TACCTCGACG
AGATCATTGA
GCAGATTTCC
GAGTTCTCCA
AGCGCGTGAT
CCTGGCCGAC
GCGAATCTGG
ATAAGGTCCT
CTCCGCGTAC
AACAAGCACC
GCGACAAGCC
AATCAGGGAG
CAGGCTGAGA
ATATCATTCA
TCTCTTCACC
CTGACGAACC
TCGGCGCCCC
TGCTGCTTTC
AAGTACTTCG
ACACAACTAT
CGATCGCAAG
AGGTACACAA
GCACTAAGGA
GGTCCTGGAC
GCGACCCTCA
TCCACCAGTC
GATTACCGGC
CTCTACGAGA
CGCGCATCGA
CCTGTCTCAG
CTCGGGGGCG
ACAAGCGGCC
AGCGGCGACG
AAGAAGGCGG
GGCAGGCGAA
GAAGAAGAAG
TCCGGGTCCT
CGGGGTCGCC
AAAGAAGAAG
CGGAAGGTCG
GCTCAAGCGG
GTCACCAAAG
AAGAAGCGGA
AGGTCGGCTC
GATGGACGCT
AAGTCCCTCA
CCGCTTGGAG
CCGCACCCTG
GTGACGTTCA
AGGACGTGTT
CGTCGATTTC
ACAAGGGAGG
AGTGGAAGCT
CCTGGACACT
GCTCAGCAGA
TCGTTTACCG
GAACGTGATG
CTGGAGAACT
ACAAGAACCT
CGTGTCCCTC
GGCTACCAGC
TGACGAAGCC
GGATGTTATT
CTCCGCCTGG
AGAAGGGCGA
GGAGCCCGGG
TCCCCTAAGA
AGAAGCGGAA
GGTCGGCTCC
TACCCATACG
ATGTCCCTGA
CTACGCCTCA
TGAGCTCAGG
CCTCCATACA
AGTATTGGGG
ATCCGAATTT
CCCCGATCGT
TCAAACATTT
GGCAATAAAG
TTTCTTAAGA
TTGAATCCTG
TTGCCGGTCT
TGCGATGATT
ATCATATAAT
TTCTGTTGAA
TTACGTTAAG
CATGTAATAA
TTAACATGTA
ATGCATGACG
TTATTTATGA
GATGGGTTTT
TATGATTAGA
GTCCCGCAAT
TATACATTTA
ATACGCGATA
GAAAACAAAA
TATAGCGCGC
AAACTAGGAT
AAATTATCGC
GCGCGGTGTC
ATCTATGTTA
CTAGATCGGG
AATTCACTGG
CCGTCGTTTT
ACAACGTCGT
GACTGGGAAA
ACCCTGGCGT
TACCCAACTT
AATCGCCTTG
CAGCACATCC
CCCTTTCGCC
AGGGGTACCG
AGCTCTAGAA
TTCGAGGCGG
TTTGCGTATT
GGCTAGAGCA
GCTTGCCAAC
ATGGTGGAGC
ACGACACTCT
CGTCTACTCC
AAGAATATCA
AAGATACAGT
CTCAGAAGAC
CAAAGGGCTA
TTGAGACTTT
TCAACAAAGG
GTAATATCGG
GAAACCTCCT
CGGATTCCAT
TGCCCAGCTA
TCTGTCACTT
CATCAAAAGG
ACAGTAGAAA
AGGAAGGTGG
CACCTACAAA
TGCCATCATT
GCGATAAAGG
AAAGGCTATC
GTTCAAGATG
CCTCTGCCGA
CAGTGGTCCC
AAAGATGGAC
CCCCACCCAC
GAGGAGCATC
GTGGAAAAAG
AAGACGTTCC
AACCACGTCT
TCAAAGCAAG
TGGATTGATG
TGATAACATG
GTGGAGCACG
ACACTCTCGT
CTACTCCAAG
AATATCAAAG
ATACAGTCTC
AGAAGACCAA
AGGGCTATTG
AGACTTTTCA
ACAAAGGGTA
ATATCGGGAA
ACCTCCTCGG
ATTCCATTGC
CCAGCTATCT
GTCACTTCAT
CAAAAGGACA
GTAGAAAAGG
AAGGTGGCAC
CTACAAATGC
CATCATTGCG
ATAAAGGAAA
GGCTATCGTT
CAAGATGCCT
CTGCCGACAG
TGGTCCCAAA
GATGGACCCC
CACCCACGAG
GAGCATCGTG
GAAAAAGAAG
ACGTTCCAAC
CACGTCTTCA
AAGCAAGTGG
ATTGATGTGA
TATCTCCACT
GACGTAAGGG
ATGACGCACA
ATCCCACTAT
CCTTCGCAAG
ACCTTCCTCT
ATATAAGGAA
GTTCATTTCA
TTTGGAGAGG
ACACGCTGAA
ATCACCAGTC
TCTCTCTACA
AATCTATCTC
TCTCGAGTCT
ACCATGAGCC
CAGAACGACG
CCCGGCCGAC
ATCCGCCGTG
CCACCGAGGC
GGACATGCCG
GCGGTCTGCA
CCATCGTCAA
CCACTACATC
GAGACAAGCA
CGGTCAACTT
CCGTACCGAG
CCGCAGGAAC
CGCAGGAGTG
GACGGACGAC
CTCGTCCGTC
TGCGGGAGCG
CTATCCCTGG
CTCGTCGCCG
AGGTGGACGG
CGAGGTCGCC
GGCATCGCCT
ACGCGGGCCC
CTGGAAGGCA
CGCAACGCCT
ACGACTGGAC
GGCCGAGTCG
ACCGTGTACG
TCTCCCCCCG
CCACCAGCGG
ACGGGACTGG
GCTCCACGCT
CTACACCCAC
CTGCTGAAGT
CCCTGGAGGC
ACAGGGCTTC
AAGAGCGTGG
TCGCTGTCAT
CGGGCTGCCC
AACGACCCGA
GCGTGCGCAT
GCACGAGGCG
CTCGGATATG
CCCCCCGCGG
CATGCTGCGG
GCGGCCGGCT
TCAAGCACGG
GAACTGGCAT
GACGTGGGTT
TCTGGCAGCT
GGACTTCAGC
CTGCCGGTAC
CGCCCCGTCC
GGTCCTGCCC
GTCACCGAGA
TTTGACTCGA
GTTTCTCCAT
AATAATGTGT
GAGTAGTTCC
CAGATAAGGG
AATTAGGGTT
CCTATAGGGT
TTCGCTCATG
TGTTGAGCAT
ATAAGAAACC
CTTAGTATGT
ATTTGTATTT
GTAAAATACT
TCTATCAATA
AAATTTCTAA
TTCCTAAAAC
CAAAATCCAG
TACTAAAATC
CAGATCCCCC
GAATTAATTC
GGCGTTAATT
CAGTACATTA
AAAACGTCCG
CAATGTGTTA
TTAAGTTGTC
ACTAGTCAGG
TTAACTCAAT
TCGGCGTTAA
TTCAGTACAT
TAAAAACGTC
CGCAATGTGT
TATTAAGTTG
TCTAAGCGTC
AATTTGTTTA
CACCACAATA
TATCCTGCCA
CCAGCCAGCC
AACAGCTCCC
CGACCGGCAG
CTCGGCACAA
AATCACCACT
CGATACAGGC
AGCCCATCAG
TCCGGGACGG
CGTCAGCGGG
AGAGCCGTTG
TAAGGCGGCA
GACTTTGCTC
ATGTTACCGA
TGCTATTCGG
AAGAACGGCA
ACTAAGCTGC
CGGGTTTGAA
ACACGGATGA
TCTCGCGGAG
GGTAGCATGT
TGATTGTAAC
GATGACAGAG
CGTTGCTGCC
TGTGATCACT
TAAGTAACTA
ACTAACAGGA
AGAGTTTGTA
GAAACGCAAA
AAGGCCATCC
GTCAGGATGG
CCTTCTGCTT
AGTTTGATGC
CTGGCAGTTT
ATGGCGGGCG
TCCTGCCCGC
CACCCTCCGG
GCCGTTGCTT
CACAACGTTC
AAATCCGCTC
CCGGCGGATT
TGTCCTACTC
AGGAGAGCGT
TCACCGACAA
ACAACAGATA
AAACGAAAGG
CCCAGTCTTC
CGACTGAGCC
TTTCGTTTTA
TTTGATGCCT
GGCAGTTCCC
TACTCTCGCT
TAGTAGTTAG
ACGTCCCCGA
GATCCATGCT
AGACCATGAA
TCCAGAAGCC
CGAGAGGTTG
CCGCCTTTCG
GGCTTTTTCT
TTTTCAAAAA
AAAAAATTTA
TAAAACGATC
TGTTGCGGCC
GGCCGCCGGG
TTGTGGGCAA
AGGCGCTCGA
CGGTGGGCAA
CCGCTTGCGG
TTGTCCACGG
GCGGAGCCGG
TGCGCGTAGC
GCATTGTCCA
CAAGCCAAGG
GCGACCAATA
ATTGATATAT
ATATTCATAA
TTGAAAAGCT
AATTGAACAT
ACTACTTGCT
GTAACTACTT
GCCGGAGCGA
GGGGTGTTTG
CAAGCTGTTG
ATCTGAAAGG
GCTATTAGCG
TTCTCACGTG
CCTTTTTGAT
TAGCGATTTC
ACGTGACCTT
ATTAGCGATT
TCACGTACTC
CGATTAGCGA
TTTCACGTAC
CCTGATTAGC
GATTTCACGT
GGATAGTTTT
TGGAGCGGGC
CGGAAAGCCC
CGTGAATCAA
GGCTTTGCGG
GGCATTAGCG
GTTTCACGTG
GATAACTACC
CTCTATCCAC
AGGCTTCCGG
GGATAAAAAA
GCCCGCTCGA
CGGCGGGCTG
TTGGATGGGG
ATCTAGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACCGGGTC
TGACGCTCAG
TGGAACGGGG
CCCAATCTGA
ATAATGTTAC
AACCAATTAA
CCAATTCTGA
TTAGAAAAAC
TCATCGAGCA
TCAAATGAAA
CTGCAATTTA
TTCATATCAG
GATTATCAAT
ACCATATTTT
TGAAAAAGCC
GTTTCTGTAA
TGAAGGAGAA
AACTCACCGA
GGCAGTTCCA
TAGGATGGCA
AGATCCTGGT
ATCGGTCTGC
GATTCCGACT
CGTCCAACAT
CAATACAACC
TATTAATTTC
CCCTCGTCAA
AAATAAGGTT
ATCAAGTGAG
AAATCACCAT
GAGTGACGAC
TGAATCCGGT
GAGAATGGCA
AAAGTTTATG
CATTTCTTTC
CAGACTTGTT
CAACAGGCCA
GCCATTACGC
TCGTCATCAA
AATCACTCGC
ATCAACCAAA
CCGTTATTCA
TTCGTGATTG
CGCCTGAGCG
AGACGAAATA
CGCGATCGCT
GTTAAAAGGA
CAATTACAAA
CAGGAATCGA
ATGCAACCGG
CGCAGGGACA
CTGCCAGCGC
ATCAACAATA
TTTTCACCTG
AATCAGGATA
TTCTTCTAAT
ACCTGGAATG
CTGTTTTTCC
GGGGATCGCA
GTGGTGAGTA
ACCATGCATC
ATCAGGAGTA
CGGATAAAAT
GCTTGATGGT
CGGAAGAGGC
ATAAATTCCG
TCAGCCAGTT
TAGTCTGACC
ATCTCATCTG
TAACATCATT
GGCAACGCTA
CCTTTGCCAT
GTTTCAGAAA
CAACTCTGGC
GCATCGGGCT
TCCCATACAA
GCGATAGATT
GTCGCACCTG
ATTGCCCGAC
ATTATCGCGA
GCCCATTTAT
ACCCATATAA
ATCAGCATCC
ATGTTGGAAT
TTAATCGCGG
CCTCGACGTT
TCCCGTTGAA
TATGGCTCAT
AACACCCCTT
GTATTACTGT
TTATGTAAGC
AGACAGTTTT
ATTGTTCATG
ATGATATATT
TTTATCTTGT
GCAATGTAAC
ATCAGAGATT
TTGAGACACG
GGCCAGAGCT
GCAGTTTGAT
CCCGAGGGGA
ACCCTGTGGT
TGACATGCAC
ATACAAATGG
ACGAACGGAT
AAACCTTTTC
ACGCCCTTTT
AAATATCCGT
TATTCTAATA
AACGCTCTTT
TCTCTTAG