名称: | pCAMBIA1300-AtU6-26-FPA(arabidopsis)-sgRNA1-pYAO-Cas9 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 拟南芥 |
原核抗性: | Kan |
筛选标记: | Hyg |
荧光标记: | |
启动子: | ATU6 |
复制子: | pVS1 |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACTGGCCGTC
GTTTTACAAC
GTCGTGACTG
GGAAAACCCT
GGCGTTACCC
AACTTAATCG
CCTTGCAGCA
CATCCCCCTT
TCGCCAGCTG
GCGTAATAGC
GAAGAGGCCC
GCACCGATCG
CCCTTCCCAA
CAGTTGCGCA
GCCTGAATGG
CGAATGCTAG
AGCAGCTTGA
GCTTGGATCA
GATTGTCGTT
TCCCGCCTTC
AGTTTAAACT
ATCAGTGTTT
GACAGGATAT
ATTGGCGGGT
AAACCTAAGA
GAAAAGAGCG
TTTATTAGAA
TAACGGATAT
TTAAAAGGGC
GTGAAAAGGT
TTATCCGTTC
GTCCATTTGT
ATGTGCATGC
CAACCACAGG
GTTCCCCTCG
GGATCAAAGT
ACTTTGATCC
AACCCCTCCG
CTGCTATAGT
GCAGTCGGCT
TCTGACGTTC
AGTGCAGCCG
TCTTCTGAAA
ACGACATGTC
GCACAAGTCC
TAAGTTACGC
GACAGGCTGC
CGCCCTGCCC
TTTTCCTGGC
GTTTTCTTGT
CGCGTGTTTT
AGTCGCATAA
AGTAGAATAC
TTGCGACTAG
AACCGGAGAC
ATTACGCCAT
GAACAAGAGC
GCCGCCGCTG
GCCTGCTGGG
CTATGCCCGC
GTCAGCACCG
ACGACCAGGA
CTTGACCAAC
CAACGGGCCG
AACTGCACGC
GGCCGGCTGC
ACCAAGCTGT
TTTCCGAGAA
GATCACCGGC
ACCAGGCGCG
ACCGCCCGGA
GCTGGCCAGG
ATGCTTGACC
ACCTACGCCC
TGGCGACGTT
GTGACAGTGA
CCAGGCTAGA
CCGCCTGGCC
CGCAGCACCC
GCGACCTACT
GGACATTGCC
GAGCGCATCC
AGGAGGCCGG
CGCGGGCCTG
CGTAGCCTGG
CAGAGCCGTG
GGCCGACACC
ACCACGCCGG
CCGGCCGCAT
GGTGTTGACC
GTGTTCGCCG
GCATTGCCGA
GTTCGAGCGT
TCCCTAATCA
TCGACCGCAC
CCGGAGCGGG
CGCGAGGCCG
CCAAGGCCCG
AGGCGTGAAG
TTTGGCCCCC
GCCCTACCCT
CACCCCGGCA
CAGATCGCGC
ACGCCCGCGA
GCTGATCGAC
CAGGAAGGCC
GCACCGTGAA
AGAGGCGGCT
GCACTGCTTG
GCGTGCATCG
CTCGACCCTG
TACCGCGCAC
TTGAGCGCAG
CGAGGAAGTG
ACGCCCACCG
AGGCCAGGCG
GCGCGGTGCC
TTCCGTGAGG
ACGCATTGAC
CGAGGCCGAC
GCCCTGGCGG
CCGCCGAGAA
TGAACGCCAA
GAGGAACAAG
CATGAAACCG
CACCAGGACG
GCCAGGACGA
ACCGTTTTTC
ATTACCGAAG
AGATCGAGGC
GGAGATGATC
GCGGCCGGGT
ACGTGTTCGA
GCCGCCCGCG
CACGTCTCAA
CCGTGCGGCT
GCATGAAATC
CTGGCCGGTT
TGTCTGATGC
CAAGCTGGCG
GCCTGGCCGG
CCAGCTTGGC
CGCTGAAGAA
ACCGAGCGCC
GCCGTCTAAA
AAGGTGATGT
GTATTTGAGT
AAAACAGCTT
GCGTCATGCG
GTCGCTGCGT
ATATGATGCG
ATGAGTAAAT
AAACAAATAC
GCAAGGGGAA
CGCATGAAGG
TTATCGCTGT
ACTTAACCAG
AAAGGCGGGT
CAGGCAAGAC
GACCATCGCA
ACCCATCTAG
CCCGCGCCCT
GCAACTCGCC
GGGGCCGATG
TTCTGTTAGT
CGATTCCGAT
CCCCAGGGCA
GTGCCCGCGA
TTGGGCGGCC
GTGCGGGAAG
ATCAACCGCT
AACCGTTGTC
GGCATCGACC
GCCCGACGAT
TGACCGCGAC
GTGAAGGCCA
TCGGCCGGCG
CGACTTCGTA
GTGATCGACG
GAGCGCCCCA
GGCGGCGGAC
TTGGCTGTGT
CCGCGATCAA
GGCAGCCGAC
TTCGTGCTGA
TTCCGGTGCA
GCCAAGCCCT
TACGACATAT
GGGCCACCGC
CGACCTGGTG
GAGCTGGTTA
AGCAGCGCAT
TGAGGTCACG
GATGGAAGGC
TACAAGCGGC
CTTTGTCGTG
TCGCGGGCGA
TCAAAGGCAC
GCGCATCGGC
GGTGAGGTTG
CCGAGGCGCT
GGCCGGGTAC
GAGCTGCCCA
TTCTTGAGTC
CCGTATCACG
CAGCGCGTGA
GCTACCCAGG
CACTGCCGCC
GCCGGCACAA
CCGTTCTTGA
ATCAGAACCC
GAGGGCGACG
CTGCCCGCGA
GGTCCAGGCG
CTGGCCGCTG
AAATTAAATC
AAAACTCATT
TGAGTTAATG
AGGTAAAGAG
AAAATGAGCA
AAAGCACAAA
CACGCTAAGT
GCCGGCCGTC
CGAGCGCACG
CAGCAGCAAG
GCTGCAACGT
TGGCCAGCCT
GGCAGACACG
CCAGCCATGA
AGCGGGTCAA
CTTTCAGTTG
CCGGCGGAGG
ATCACACCAA
GCTGAAGATG
TACGCGGTAC
GCCAAGGCAA
GACCATTACC
GAGCTGCTAT
CTGAATACAT
CGCGCAGCTA
CCAGAGTAAA
TGAGCAAATG
AATAAATGAG
TAGATGAATT
TTAGCGGCTA
AAGGAGGCGG
CATGGAAAAT
CAAGAACAAC
CAGGCACCGA
CGCCGTGGAA
TGCCCCATGT
GTGGAGGAAC
GGGCGGTTGG
CCAGGCGTAA
GCGGCTGGGT
TGTCTGCCGG
CCCTGCAATG
GCACTGGAAC
CCCCAAGCCC
GAGGAATCGG
CGTGACGGTC
GCAAACCATC
CGGCCCGGTA
CAAATCGGCG
CGGCGCTGGG
TGATGACCTG
GTGGAGAAGT
TGAAGGCCGC
GCAGGCCGCC
CAGCGGCAAC
GCATCGAGGC
AGAAGCACGC
CCCGGTGAAT
CGTGGCAAGC
GGCCGCTGAT
CGAATCCGCA
AAGAATCCCG
GCAACCGCCG
GCAGCCGGTG
CGCCGTCGAT
TAGGAAGCCG
CCCAAGGGCG
ACGAGCAACC
AGATTTTTTC
GTTCCGATGC
TCTATGACGT
GGGCACCCGC
GATAGTCGCA
GCATCATGGA
CGTGGCCGTT
TTCCGTCTGT
CGAAGCGTGA
CCGACGAGCT
GGCGAGGTGA
TCCGCTACGA
GCTTCCAGAC
GGGCACGTAG
AGGTTTCCGC
AGGGCCGGCC
GGCATGGCCA
GTGTGTGGGA
TTACGACCTG
GTACTGATGG
CGGTTTCCCA
TCTAACCGAA
TCCATGAACC
GATACCGGGA
AGGGAAGGGA
GACAAGCCCG
GCCGCGTGTT
CCGTCCACAC
GTTGCGGACG
TACTCAAGTT
CTGCCGGCGA
GCCGATGGCG
GAAAGCAGAA
AGACGACCTG
GTAGAAACCT
GCATTCGGTT
AAACACCACG
CACGTTGCCA
TGCAGCGTAC
GAAGAAGGCC
AAGAACGGCC
GCCTGGTGAC
GGTATCCGAG
GGTGAAGCCT
TGATTAGCCG
CTACAAGATC
GTAAAGAGCG
AAACCGGGCG
GCCGGAGTAC
ATCGAGATCG
AGCTAGCTGA
TTGGATGTAC
CGCGAGATCA
CAGAAGGCAA
GAACCCGGAC
GTGCTGACGG
TTCACCCCGA
TTACTTTTTG
ATCGATCCCG
GCATCGGCCG
TTTTCTCTAC
CGCCTGGCAC
GCCGCGCCGC
AGGCAAGGCA
GAAGCCAGAT
GGTTGTTCAA
GACGATCTAC
GAACGCAGTG
GCAGCGCCGG
AGAGTTCAAG
AAGTTCTGTT
TCACCGTGCG
CAAGCTGATC
GGGTCAAATG
ACCTGCCGGA
GTACGATTTG
AAGGAGGAGG
CGGGGCAGGC
TGGCCCGATC
CTAGTCATGC
GCTACCGCAA
CCTGATCGAG
GGCGAAGCAT
CCGCCGGTTC
CTAATGTACG
GAGCAGATGC
TAGGGCAAAT
TGCCCTAGCA
GGGGAAAAAG
GTCGAAAAGG
TCTCTTTCCT
GTGGATAGCA
CGTACATTGG
GAACCCAAAG
CCGTACATTG
GGAACCGGAA
CCCGTACATT
GGGAACCCAA
AGCCGTACAT
TGGGAACCGG
TCACACATGT
AAGTGACTGA
TATAAAAGAG
AAAAAAGGCG
ATTTTTCCGC
CTAAAACTCT
TTAAAACTTA
TTAAAACTCT
TAAAACCCGC
CTGGCCTGTG
CATAACTGTC
TGGCCAGCGC
ACAGCCGAAG
AGCTGCAAAA
AGCGCCTACC
CTTCGGTCGC
TGCGCTCCCT
ACGCCCCGCC
GCTTCGCGTC
GGCCTATCGC
GGCCGCTGGC
CGCTCAAAAA
TGGCTGGCCT
ACGGCCAGGC
AATCTACCAG
GGCGCGGACA
AGCCGCGCCG
TCGCCACTCG
ACCGCCGGCG
CCCACATCAA
GGCACCCTGC
CTCGCGCGTT
TCGGTGATGA
CGGTGAAAAC
CTCTGACACA
TGCAGCTCCC
GGAGACGGTC
ACAGCTTGTC
TGTAAGCGGA
TGCCGGGAGC
AGACAAGCCC
GTCAGGGCGC
GTCAGCGGGT
GTTGGCGGGT
GTCGGGGCGC
AGCCATGACC
CAGTCACGTA
GCGATAGCGG
AGTGTATACT
GGCTTAACTA
TGCGGCATCA
GAGCAGATTG
TACTGAGAGT
GCACCATATG
CGGTGTGAAA
TACCGCACAG
ATGCGTAAGG
AGAAAATACC
GCATCAGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGGACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGCAT
TCTAGGTACT
AAAACAATTC
ATCCAGTAAA
ATATAATATT
TTATTTTCTC
CCAATCAGGC
TTGATCCCCA
GTAAGTCAAA
AAATAGCTCG
ACATACTGTT
CTTCCCCGAT
ATCCTCCCTG
ATCGACCGGA
CGCAGAAGGC
AATGTCATAC
CACTTGTCCG
CCCTGCCGCT
TCTCCCAAGA
TCAATAAAGC
CACTTACTTT
GCCATCTTTC
ACAAAGATGT
TGCTGTCTCC
CAGGTCGCCG
TGGGAAAAGA
CAAGTTCCTC
TTCGGGCTTT
TCCGTCTTTA
AAAAATCATA
CAGCTCGCGC
GGATCTTTAA
ATGGAGTGTC
TTCTTCCCAG
TTTTCGCAAT
CCACATCGGC
CAGATCGTTA
TTCAGTAAGT
AATCCAATTC
GGCTAAGCGG
CTGTCTAAGC
TATTCGTATA
GGGACAATCC
GATATGTCGA
TGGAGTGAAA
GAGCCTGATG
CACTCCGCAT
ACAGCTCGAT
AATCTTTTCA
GGGCTTTGTT
CATCTTCATA
CTCTTCCGAG
CAAAGGACGC
CATCGGCCTC
ACTCATGAGC
AGATTGCTCC
AGCCATCATG
CCGTTCAAAG
TGCAGGACCT
TTGGAACAGG
CAGCTTTCCT
TCCAGCCATA
GCATCATGTC
CTTTTCCCGT
TCCACATCAT
AGGTGGTCCC
TTTATACCGG
CTGTCCGTCA
TTTTTAAATA
TAGGTTTTCA
TTTTCTCCCA
CCAGCTTATA
TACCTTAGCA
GGAGACATTC
CTTCCGTATC
TTTTACGCAG
CGGTATTTTT
CGATCAGTTT
TTTCAATTCC
GGTGATATTC
TCATTTTAGC
CATTTATTAT
TTCCTTCCTC
TTTTCTACAG
TATTTAAAGA
TACCCCAAGA
AGCTAATTAT
AACAAGACGA
ACTCCAATTC
ACTGTTCCTT
GCATTCTAAA
ACCTTAAATA
CCAGAAAACA
GCTTTTTCAA
AGTTGTTTTC
AAAGTTGGCG
TATAACATAG
TATCGACGGA
GCCGATTTTG
AAACCGCGGT
GATCACAGGC
AGCAACGCTC
TGTCATCGTT
ACAATCAACA
TGCTACCCTC
CGCGAGATCA
TCCGTGTTTC
AAACCCGGCA
GCTTAGTTGC
CGTTCTTCCG
AATAGCATCG
GTAACATGAG
CAAAGTCTGC
CGCCTTACAA
CGGCTCTCCC
GCTGACGCCG
TCCCGGACTG
ATGGGCTGCC
TGTATCGAGT
GGTGATTTTG
TGCCGAGCTG
CCGGTCGGGG
AGCTGTTGGC
TGGCTGGTGG
CAGGATATAT
TGTGGTGTAA
ACAAATTGAC
GCTTAGACAA
CTTAATAACA
CATTGCGGAC
GTTTTTAATG
TACTGAATTA
ACGCCGAATT
AATTCGGGGG
ATCTGGATTT
TAGTACTGGA
TTTTGGTTTT
AGGAATTAGA
AATTTTATTG
ATAGAAGTAT
TTTACAAATA
CAAATACATA
CTAAGGGTTT
CTTATATGCT
CAACACATGA
GCGAAACCCT
ATAGGAACCC
TAATTCCCTT
ATCTGGGAAC
TACTCACACA
TTATTATGGA
GAAACTCGAG
CTTGTCGATC
GACAGATCCG
GTCGGCATCT
ACTCTATTTC
TTTGCCCTCG
GACGAGTGCT
GGGGCGTCGG
TTTCCACTAT
CGGCGAGTAC
TTCTACACAG
CCATCGGTCC
AGACGGCCGC
GCTTCTGCGG
GCGATTTGTG
TACGCCCGAC
AGTCCCGGCT
CCGGATCGGA
CGATTGCGTC
GCATCGACCC
TGCGCCCAAG
CTGCATCATC
GAAATTGCCG
TCAACCAAGC
TCTGATAGAG
TTGGTCAAGA
CCAATGCGGA
GCATATACGC
CCGGAGTCGT
GGCGATCCTG
CAAGCTCCGG
ATGCCTCCGC
TCGAAGTAGC
GCGTCTGCTG
CTCCATACAA
GCCAACCACG
GCCTCCAGAA
GAAGATGTTG
GCGACCTCGT
ATTGGGAATC
CCCGAACATC
GCCTCGCTCC
AGTCAATGAC
CGCTGTTATG
CGGCCATTGT
CCGTCAGGAC
ATTGTTGGAG
CCGAAATCCG
CGTGCACGAG
GTGCCGGACT
TCGGGGCAGT
CCTCGGCCCA
AAGCATCAGC
TCATCGAGAG
CCTGCGCGAC
GGACGCACTG
ACGGTGTCGT
CCATCACAGT
TTGCCAGTGA
TACACATGGG
GATCAGCAAT
CGCGCATATG
AAATCACGCC
ATGTAGTGTA
TTGACCGATT
CCTTGCGGTC
CGAATGGGCC
GAACCCGCTC
GTCTGGCTAA
GATCGGCCGC
AGCGATCGCA
TCCATAGCCT
CCGCGACCGG
TTGTAGAACA
GCGGGCAGTT
CGGTTTCAGG
CAGGTCTTGC
AACGTGACAC
CCTGTGCACG
GCGGGAGATG
CAATAGGTCA
GGCTCTCGCT
AAACTCCCCA
ATGTCAAGCA
CTTCCGGAAT
CGGGAGCGCG
GCCGATGCAA
AGTGCCGATA
AACATAACGA
TCTTTGTAGA
AACCATCGGC
GCAGCTATTT
ACCCGCAGGA
CATATCCACG
CCCTCCTACA
TCGAAGCTGA
AAGCACGAGA
TTCTTCGCCC
TCCGAGAGCT
GCATCAGGTC
GGAGACGCTG
TCGAACTTTT
CGATCAGAAA
CTTCTCGACA
GACGTCGCGG
TGAGTTCAGG
CTTTTTCATA
TCTCATTGCC
CCCCGGGATC
TGCGAAAGCT
CGAGAGAGAT
AGATTTGTAG
AGAGAGACTG
GTGATTTCAG
CGTGTCCTCT
CCAAATGAAA
TGAACTTCCT
TATATAGAGG
AAGGTCTTGC
GAAGGATAGT
GGGATTGTGC
GTCATCCCTT
ACGTCAGTGG
AGATATCACA
TCAATCCACT
TGCTTTGAAG
ACGTGGTTGG
AACGTCTTCT
TTTTCCACGA
TGCTCCTCGT
GGGTGGGGGT
CCATCTTTGG
GACCACTGTC
GGCAGAGGCA
TCTTGAACGA
TAGCCTTTCC
TTTATCGCAA
TGATGGCATT
TGTAGGTGCC
ACCTTCCTTT
TCTACTGTCC
TTTTGATGAA
GTGACAGATA
GCTGGGCAAT
GGAATCCGAG
GAGGTTTCCC
GATATTACCC
TTTGTTGAAA
AGTCTCAATA
GCCCTTTGGT
CTTCTGAGAC
TGTATCTTTG
ATATTCTTGG
AGTAGACGAG
AGTGTCGTGC
TCCACCATGT
TATCACATCA
ATCCACTTGC
TTTGAAGACG
TGGTTGGAAC
GTCTTCTTTT
TCCACGATGC
TCCTCGTGGG
TGGGGGTCCA
TCTTTGGGAC
CACTGTCGGC
AGAGGCATCT
TGAACGATAG
CCTTTCCTTT
ATCGCAATGA
TGGCATTTGT
AGGTGCCACC
TTCCTTTTCT
ACTGTCCTTT
TGATGAAGTG
ACAGATAGCT
GGGCAATGGA
ATCCGAGGAG
GTTTCCCGAT
ATTACCCTTT
GTTGAAAAGT
CTCAATAGCC
CTTTGGTCTT
CTGAGACTGT
ATCTTTGATA
TTCTTGGAGT
AGACGAGAGT
GTCGTGCTCC
ACCATGTTGG
CAAGCTGCTC
TAGCCAATAC
GCAAACCGCC
TCTCCCCGCG
CGTTGGCCGA
TTCATTAATG
CAGCTGGCAC
GACAGGTTTC
CCGACTGGAA
AGCGGGCAGT
GAGCGCAACG
CAATTAATGT
GAGTTAGCTC
ACTCATTAGG
CACCCCAGGC
TTTACACTTT
ATGCTTCCGG
CTCGTATGTT
GTGTGGAATT
GTGAGCGGAT
AACAATTTCA
CACAGGAAAC
AGCTATGACA
TGATTACGAA
TTCCCGATCT
AGTAACATAG
ATGACACCGC
GCGCGATAAT
TTATCCTAGT
TTGCGCGCTA
TATTTTGTTT
TCTATCGCGT
ATTAAATGTA
TAATTGCGGG
ACTCTAATCA
TAAAAACCCA
TCTCATAAAT
AACGTCATGC
ATTACATGTT
AATTATTACA
TGCTTAACGT
AATTCAACAG
AAATTATATG
ATAATCATCG
CAAGACCGGC
AACAGGATTC
AATCTTAAGA
AACTTTATTG
CCAAATGTTT
GAACGATCGG
GGAAATTCGA
GCTCTATCGA
TCAATCAGGA
TCCTTACTTT
TTCTTTTTTG
CCTGGCCGGC
CTTTTTCGTG
GCCGCCGGCC
TTTTGTCGCC
TCCCAGCTGA
GACAGGTCGA
TCCGTGTCTC
GTACAGGCCG
GTGATGCTCT
GGTGGATCAG
GGTGGCGTCC
AGCACCTCTT
TGGTGCTGGT
GTACCTCTTC
CGGTCGATGG
TGGTGTCAAA
GTACTTGAAG
GCGGCAGGGG
CTCCCAGATT
GGTCAGGGTA
AACAGGTGGA
TGATATTCTC
GGCCTGCTCT
CTGATGGGCT
TATCCCGGTG
CTTGTTGTAG
GCGGACAGCA
CTTTGTCCAG
ATTAGCGTCG
GCCAGGATCA
CTCTCTTGGA
GAACTCGCTG
ATCTGCTCGA
TGATCTCGTC
CAGGTAGTGC
TTGTGCTGTT
CCACAAACAG
CTGTTTCTGC
TCATTATCCT
CGGGGGAGCC
CTTCAGCTTC
TCATAGTGGC
TGGCCAGGTA
CAGGAAGTTC
ACATATTTGG
AGGGCAGGGC
CAGTTCGTTT
CCCTTCTGCA
GTTCGCCGGC
AGAGGCCAGC
ATTCTCTTCC
GGCCGTTTTC
CAGCTCGAAC
AGGGAGTACT
TAGGCAGCTT
GATGATCAGG
TCCTTTTTCA
CTTCTTTGTA
GCCCTTGGCT
TCCAGAAAGT
CGATGGGATT
CTTCTCGAAG
CTGCTTCTTT
CCATGATGGT
GATCCCCAGC
AGCTCTTTCA
CACTCTTCAG
TTTCTTGGAC
TTGCCCTTTT
CCACTTTGGC
CACCACCAGC
ACAGAATAGG
CCACGGTGGG
GCTGTCGAAG
CCGCCGTACT
TCTTAGGGTC
CCAGTCCTTC
TTTCTGGCGA
TCAGCTTATC
GCTGTTCCTC
TTGGGCAGGA
TAGACTCTTT
GCTGAAGCCG
CCTGTCTGCA
CCTCGGTCTT
TTTCACGATA
TTCACTTGGG
GCATGCTCAG
CACTTTCCGC
ACGGTGGCAA
AATCCCGGCC
CTTATCCCAC
ACGATCTCCC
CGGTTTCGCC
GTTTGTCTCG
ATCAGAGGCC
GCTTCCGGAT
CTCGCCGTTG
GCCAGGGTAA
TCTCGGTCTT
GAAAAAGTTC
ATGATGTTGC
TGTAGAAGAA
GTACTTGGCG
GTAGCCTTGC
CGATTTCCTG
CTCGCTCTTG
GCGATCATCT
TCCGCACGTC
GTACACCTTG
TAGTCGCCGT
ACACGAACTC
GCTTTCCAGC
TTAGGGTACT
TTTTGATCAG
GGCGGTTCCC
ACGACGGCGT
TCAGGTAGGC
GTCGTGGGCG
TGGTGGTAGT
TGTTGATCTC
GCGCACTTTG
TAAAACTGGA
AATCCTTCCG
GAAATCGGAC
ACCAGCTTGG
ACTTCAGGGT
GATCACTTTC
ACTTCCCGGA
TCAGCTTGTC
ATTCTCGTCG
TACTTAGTGT
TCATCCGGGA
GTCCAGGATC
TGTGCCACGT
GCTTTGTGAT
CTGCCGGGTT
TCCACCAGCT
GTCTCTTGAT
GAAGCCGGCC
TTATCCAGTT
CGCTCAGGCC
GCCTCTCTCG
GCCTTGGTCA
GATTGTCGAA
CTTTCTCTGG
GTAATCAGCT
TGGCGTTCAG
CAGCTGCCGC
CAGTAGTTCT
TCATCTTCTT
CACGACCTCT
TCGGAGGGCA
CGTTGTCGCT
CTTGCCCCGG
TTCTTGTCGC
TTCTGGTCAG
CACCTTGTTG
TCGATGGAGT
CGTCCTTCAG
AAAGCTCTGA
GGCACGATAT
GGTCCACATC
GTAGTCGGAC
AGCCGGTTGA
TGTCCAGTTC
CTGGTCCACG
TACATATCCC
GCCCATTCTG
CAGGTAGTAC
AGGTACAGCT
TCTCGTTCTG
CAGCTGGGTG
TTTTCCACGG
GGTGTTCTTT
CAGGATCTGG
CTGCCCAGCT
CTTTGATGCC
CTCTTCGATC
CGCTTCATTC
TCTCGCGGCT
GTTCTTCTGT
CCCTTCTGGG
TGGTCTGGTT
CTCTCTGGCC
ATTTCGATCA
CGATGTTCTC
GGGCTTGTGC
CGGCCCATCA
CTTTCACGAG
CTCGTCCACC
ACCTTCACTG
TCTGCAGGAT
GCCCTTCTTA
ATGGCGGGGC
TGCCGGCCAG
ATTGGCAATG
TGCTCGTGCA
GGCTATCGCC
CTGGCCGGAC
ACCTGGGCTT
TCTGGATGTC
CTCTTTAAAG
GTCAGGCTGT
CGTCGTGGAT
CAGCTGCATG
AAGTTTCTGT
TGGCGAAGCC
GTCGGACTTC
AGGAAATCCA
GGATTGTCTT
GCCGGACTGC
TTGTCCCGGA
TGCCGTTGAT
CAGCTTCCGG
CTCAGCCTGC
CCCAGCCGGT
GTATCTCCGC
CGCTTCAGCT
GCTTCATCAC
TTTGTCGTCG
AACAGGTGGG
CATAGGTTTT
CAGCCGTTCC
TCGATCATCT
CTCTGTCCTC
AAACAGTGTC
AGGGTCAGCA
CGATATCTTC
CAGAATGTCC
TCGTTTTCCT
CATTGTCCAG
GAAGTCCTTG
TCCTTGATAA
TTTTCAGCAG
ATCGTGGTAT
GTGCCCAGGG
AGGCGTTGAA
CCGATCTTCC
ACGCCGGAGA
TTTCCACGGA
GTCGAAGCAC
TCGATTTTCT
TGAAGTAGTC
CTCTTTCAGC
TGCTTCACGG
TCACTTTCCG
GTTGGTCTTG
AACAGCAGGT
CCACGATGGC
CTTTTTCTGC
TCGCCGCTCA
GGAAGGCGGG
CTTTCTCATT
CCCTCGGTCA
CGTATTTCAC
TTTGGTCAGC
TCGTTATACA
CGGTGAAGTA
CTCGTACAGC
AGGCTGTGCT
TGGGCAGCAC
CTTCTCGTTG
GGCAGGTTCT
TATCGAAGTT
GGTCATCCGC
TCGATGAAGC
TCTGGGCGGA
AGCGCCCTTG
TCCACCACTT
CCTCGAAGTT
CCAGGGGGTG
ATGGTTTCCT
CGCTCTTTCT
GGTCATCCAG
GCGAATCTGC
TGTTTCCCCT
GGCCAGAGGG
CCCACGTAGT
AGGGGATGCG
GAAGGTCAGG
ATCTTCTCGA
TCTTTTCCCG
GTTGTCCTTC
AGGAATGGGT
AAAAATCTTC
CTGCCGCCGC
AGAATGGCGT
GCAGCTCTCC
CAGGTGGATC
TGGTGGGGGA
TGCTGCCGTT
GTCGAAGGTC
CGCTGCTTCC
GCAGCAGGTC
CTCTCTGTTC
AGCTTCACGA
GCAGTTCCTC
GGTGCCGTCC
ATCTTTTCCA
GGATGGGCTT
GATGAACTTG
TAGAACTCTT
CCTGGCTGGC
TCCGCCGTCA
ATGTAGCCGG
CGTAGCCGTT
CTTGCTCTGG
TCGAAGAAAA
TCTCTTTGTA
CTTCTCAGGC
AGCTGCTGCC
GCACGAGAGC
TTTCAGCAGG
GTCAGGTCCT
GGTGGTGCTC
GTCGTATCTC
TTGATCATAG
AGGCGCTCAG
GGGGGCCTTG
GTGATCTCGG
TGTTCACTCT
CAGGATGTCG
CTCAGCAGGA
TGGCGTCGGA
CAGGTTCTTG
GCGGCCAGAA
ACAGGTCGGC
GTACTGGTCG
CCGATCTGGG
CCAGCAGGTT
GTCCAGGTCG
TCGTCGTAGG
TGTCCTTGCT
CAGCTGCAGT
TTGGCATCCT
CGGCCAGGTC
GAAGTTGCTC
TTGAAGTTGG
GGGTCAGGCC
CAGGCTCAGG
GCAATCAGGT
TTCCGAACAG
GCCATTCTTC
TTCTCGCCGG
GCAGCTGGGC
GATCAGATTT
TCCAGCCGTC
TGCTCTTGCT
CAGTCTGGCA
GACAGGATGG
CCTTGGCGTC
CACGCCGCTG
GCGTTGATGG
GGTTTTCCTC
GAACAGCTGG
TTGTAGGTCT
GCACCAGCTG
GATGAACAGC
TTGTCCACGT
CGCTGTTGTC
GGGGTTCAGG
TCGCCCTCGA
TCAGGAAGTG
GCCCCGGAAC
TTGATCATGT
GGGCCAGGGC
CAGATAGATC
AGCCGCAGGT
CGGCCTTGTC
GGTGCTGTCC
ACCAGTTTCT
TTCTCAGGTG
GTAGATGGTG
GGGTACTTCT
CGTGGTAGGC
CACCTCGTCC
ACGATGTTGC
CGAAGATGGG
GTGCCGCTCG
TGCTTCTTAT
CCTCTTCCAC
CAGGAAGGAC
TCTTCCAGTC
TGTGGAAGAA
GCTGTCGTCC
ACCTTGGCCA
TCTCGTTGCT
GAAGATCTCT
TGCAGATAGC
AGATCCGGTT
CTTCCGTCTG
GTGTATCTTC
TTCTGGCGGT
TCTCTTCAGC
CGGGTGGCCT
CGGCTGTTTC
GCCGCTGTCG
AACAGCAGGG
CTCCGATCAG
GTTCTTCTTG
ATGCTGTGCC
GGTCGGTGTT
GCCCAGCACC
TTGAATTTCT
TGCTGGGCAC
CTTGTACTCG
TCGGTGATCA
CGGCCCAGCC
CACAGAGTTG
GTGCCGATGT
CCAGGCCGAT
GCTGTACTTC
TTGTCGGCTG
CTGGGACTCC
GTGGATACCG
ACCTTCCGCT
TCTTCTTTGG
GGCCATCTTA
TCGTCATCGT
CTTTGTAATC
AATATCATGA
TCCTTGTAGT
CTCCGTCGTG
GTCCTTATAG
TCCATCTCGA
CTCCTTTCTT
CTTCTCGTTG
TTGTACTTCA
TTCTTCTCTC
TCTCACTCCC
TCTTAGGGTT
TAACGATTTT
ACACTTTGAC
TCGTCTAGGT
TTAGCCTCAG
GGTTTAGTGG
CGACGGAAGA
AGCTGGAAAA
CGATTTTGTT
TATTTGGTCG
GAAAGTTTAC
AACTTTAAAC
CTTCCACTAG
GTTTTCTTCA
GGAATCGGGT
CTACCGGGTT
GACCCGGACT
AACCAAGCCC
ATGACTTACC
TGGTCTATGG
TTTGAGCCCA
TTAACTATTT
CGTTCTTTTA
AAGCAGAAAC
TTGGGCCTTT
AATTTGTAGA
GCCGAAGACT
AACCGGACCG
TCAATTTAAG
TTTGGTTCAA
TCCGATTTCT
GAATTTTTGG
TTCTGATTTC
TGAAGAGTAG
TACGTAAACC
GAATTGGGAG
ATTCTCTTAT
TTCAGTAACA
GTTTAATACA
GTTTTTGTTT
AGTGTTTTCA
ATTTCGGTAT
TTTTTACAAA
CTCGAATCGG
ATGATTCCGG
TTTAATAAAA
TCAAATGAGT
TCGGTTTAGC
TGAATTTAAA
ACTGTTTTTA
ATCTTATAGT
ATACCGAACC
GGACCATCCA
TCTAATTTCA
ATTGTCTACC
AAAACCGACC
GACCAGTCTT
TGTCGAAAGT
TGTCTTTCTT
CTTCCTTGTT
CGATTTTCGC
CTCCTTTGGC
TTTTCATTCT
CTCTCTAACT
TCTCTATGTT
AAAATTCCGA
AAGCTCGATT
CAGAAATCTA
GAAGCTTCCT
CGAGGGCCCT
CGCGTAGAAA
GTCGAACTCA
AATTCCCTGG
AGACAAAGAA
GAGGAAGATG
AGCTCATGGA
ACTACGTTGT
TACGGCTCAC
AAACCGACCA
GTGTCACTCA
CTCGTGCGTT
GGCAACTTCA
CAAGTCCTCA
AGAGCTCAAT
CTCATTGTCG
CGTACGAAAC
TCTCCCCCTA
CCTCTATTTG
TTATCAATTT
TTGTTTGTCA
ATTAGTCCCC
TTTTGAATTG
CAGCTGTTGA
TTTGGGTTTA
GGGTTTTCAA
TGAATTTCCC
ATCGTCGAGG
GGGGGCCCGG
TACCTTCCTA
GGATACTAGT
AGTGTGCTGG
AATTGCCCTT
AAGCTTCGTT
GAACAACGGA
AACTCGACTT
GCCTTCCGCA
CAATACATCA
TTTCTTCTTA
GCTTTTTTTC
TTCTTCTTCG
TTCATACAGT
TTTTTTTTGT
TTATCAGCTT
ACATTTTCTT
GAACCGTAGC
TTTCGTTTTC
TTCTTTTTAA
CTTTCCATTC
GGAGTTTTTG
TATCTTGTTT
CATAGTTTGT
CCCAGGATTA
GAATGATTAG
GCATCGAACC
TTCAAGAATT
TGATTGAATA
AAACATCTTC
ATTCTTAAGA
TATGAAGATA
ATCTTCAAAA
GGCCCCTGGG
AATCTGAAAG
AAGAGAAGCA
GGCCCATTTA
TATGGGAAAG
AACAATAGTA
TTTCTTATAT
AGGCCCATTT
AAGTTGAAAA
CAATCTTCAA
AAGTCCCACA
TCGCTTAGAT
AAGAAAACGA
AGCTGAGTTT
ATATACAGCT
AGAGTCGAAG
TAGTGATTGC
TCTTCCTCCA
GGTCATCCTG
TTTTAGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTATCAACT
TGAAAAAGTG
GCACCGAGTC
GGTGCTTTTT
TTGTCCCTTC
GAAGGGCAAT
TCTGCAGATA
TCCATCACAC
TGGGTCGACC
TGCAGGCATG
CAAGCTTGGC