名称: | pL-CRISPR.EFS.GFP |
---|---|
别称: | Plasmid#57818 |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 空载体 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTAATGTAGT
CTTATGCAAT
ACTCTTGTAG
TCTTGCAACA
TGGTAACGAT
GAGTTAGCAA
CATGCCTTAC
AAGGAGAGAA
AAAGCACCGT
GCATGCCGAT
TGGTGGAAGT
AAGGTGGTAC
GATCGTGCCT
TATTAGGAAG
GCAACAGACG
GGTCTGACAT
GGATTGGACG
AACCACTGAA
TTGCCGCATT
GCAGAGATAT
TGTATTTAAG
TGCCTAGCTC
GATACATAAA
CGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTGGCGCC
CGAACAGGGA
CTTGAAAGCG
AAAGGGAAAC
CAGAGGAGCT
CTCTCGACGC
AGGACTCGGC
TTGCTGAAGC
GCGCACGGCA
AGAGGCGAGG
GGCGGCGACT
GGTGAGTACG
CCAAAAATTT
TGACTAGCGG
AGGCTAGAAG
GAGAGAGATG
GGTGCGAGAG
CGTCAGTATT
AAGCGGGGGA
GAATTAGATC
GCGATGGGAA
AAAATTCGGT
TAAGGCCAGG
GGGAAAGAAA
AAATATAAAT
TAAAACATAT
AGTATGGGCA
AGCAGGGAGC
TAGAACGATT
CGCAGTTAAT
CCTGGCCTGT
TAGAAACATC
AGAAGGCTGT
AGACAAATAC
TGGGACAGCT
ACAACCATCC
CTTCAGACAG
GATCAGAAGA
ACTTAGATCA
TTATATAATA
CAGTAGCAAC
CCTCTATTGT
GTGCATCAAA
GGATAGAGAT
AAAAGACACC
AAGGAAGCTT
TAGACAAGAT
AGAGGAAGAG
CAAAACAAAA
GTAAGACCAC
CGCACAGCAA
GCGGCCGCTG
ATCTTCAGAC
CTGGAGGAGG
AGATATGAGG
GACAATTGGA
GAAGTGAATT
ATATAAATAT
AAAGTAGTAA
AAATTGAACC
ATTAGGAGTA
GCACCCACCA
AGGCAAAGAG
AAGAGTGGTG
CAGAGAGAAA
AAAGAGCAGT
GGGAATAGGA
GCTTTGTTCC
TTGGGTTCTT
GGGAGCAGCA
GGAAGCACTA
TGGGCGCAGC
GTCAATGACG
CTGACGGTAC
AGGCCAGACA
ATTATTGTCT
GGTATAGTGC
AGCAGCAGAA
CAATTTGCTG
AGGGCTATTG
AGGCGCAACA
GCATCTGTTG
CAACTCACAG
TCTGGGGCAT
CAAGCAGCTC
CAGGCAAGAA
TCCTGGCTGT
GGAAAGATAC
CTAAAGGATC
AACAGCTCCT
GGGGATTTGG
GGTTGCTCTG
GAAAACTCAT
TTGCACCACT
GCTGTGCCTT
GGAATGCTAG
TTGGAGTAAT
AAATCTCTGG
AACAGATTTG
GAATCACACG
ACCTGGATGG
AGTGGGACAG
AGAAATTAAC
AATTACACAA
GCTTAATACA
CTCCTTAATT
GAAGAATCGC
AAAACCAGCA
AGAAAAGAAT
GAACAAGAAT
TATTGGAATT
AGATAAATGG
GCAAGTTTGT
GGAATTGGTT
TAACATAACA
AATTGGCTGT
GGTATATAAA
ATTATTCATA
ATGATAGTAG
GAGGCTTGGT
AGGTTTAAGA
ATAGTTTTTG
CTGTACTTTC
TATAGTGAAT
AGAGTTAGGC
AGGGATATTC
ACCATTATCG
TTTCAGACCC
ACCTCCCAAC
CCCGAGGGGA
CCCAGAGAGG
GCCTATTTCC
CATGATTCCT
TCATATTTGC
ATATACGATA
CAAGGCTGTT
AGAGAGATAA
TTAGAATTAA
TTTGACTGTA
AACACAAAGA
TATTAGTACA
AAATACGTGA
CGTAGAAAGT
AATAATTTCT
TGGGTAGTTT
GCAGTTTTAA
AATTATGTTT
TAAAATGGAC
TATCATATGC
TTACCGTAAC
TTGAAAGTAT
TTCGATTTCT
TGGCTTTATA
TATCTTGTGG
AAAGGACGAA
ACACCGGAGA
CGTGACCGTC
TCTGTTTTAG
AGCTAGAAAT
AGCAAGTTAA
AATAAGGCTA
GTCCGTTATC
AACTTGAAAA
AGTGGCACCG
AGTCGGTGCT
TTTTTGAATT
CTAGATCTTG
AGACAAATGG
CAGTATTCAT
CCACAATTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ATTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCGGGTT
TATTACAGGG
ACAGCAGAGA
TCCACTTTGG
CGCCGGCTCG
AGTGGCTCCG
GTGCCCGTCA
GTGGGCAGAG
CGCACATCGC
CCACAGTCCC
CGAGAAGTTG
GGGGGAGGGG
TCGGCAATTG
AACCGGTGCC
TAGAGAAGGT
GGCGCGGGGT
AAACTGGGAA
AGTGATGTCG
TGTACTGGCT
CCGCCTTTTT
CCCGAGGGTG
GGGGAGAACC
GTATATAAGT
GCAGTAGTCG
CCGTGAACGT
TCTTTTTCGC
AACGGGTTTG
CCGCCAGAAC
ACAGGTGTCG
TGACGCGGGA
TCCGCCACCA
TGGATTACAA
AGACGATGAC
GATAAGATGG
CCCCAAAGAA
GAAGCGGAAG
GTCGGTATCC
ACGGAGTCCC
AGCAGCCGAC
AAGAAGTACA
GCATCGGCCT
GGACATCGGC
ACCAACTCTG
TGGGCTGGGC
CGTGATCACC
GACGAGTACA
AGGTGCCCAG
CAAGAAATTC
AAGGTGCTGG
GCAACACCGA
CCGGCACAGC
ATCAAGAAGA
ACCTGATCGG
AGCCCTGCTG
TTCGACAGCG
GCGAAACAGC
CGAGGCCACC
CGGCTGAAGA
GAACCGCCAG
AAGAAGATAC
ACCAGACGGA
AGAACCGGAT
CTGCTATCTG
CAAGAGATCT
TCAGCAACGA
GATGGCCAAG
GTGGACGACA
GCTTCTTCCA
CAGACTGGAA
GAGTCCTTCC
TGGTGGAAGA
GGATAAGAAG
CACGAGCGGC
ACCCCATCTT
CGGCAACATC
GTGGACGAGG
TGGCCTACCA
CGAGAAGTAC
CCCACCATCT
ACCACCTGAG
AAAGAAACTG
GTGGACAGCA
CCGACAAGGC
CGACCTGCGG
CTGATCTATC
TGGCCCTGGC
CCACATGATC
AAGTTCCGGG
GCCACTTCCT
GATCGAGGGC
GACCTGAACC
CCGACAACAG
CGACGTGGAC
AAGCTGTTCA
TCCAGCTGGT
GCAGACCTAC
AACCAGCTGT
TCGAGGAAAA
CCCCATCAAC
GCCAGCGGCG
TGGACGCCAA
GGCCATCCTG
TCTGCCAGAC
TGAGCAAGAG
CAGACGGCTG
GAAAATCTGA
TCGCCCAGCT
GCCCGGCGAG
AAGAAGAATG
GCCTGTTCGG
AAACCTGATT
GCCCTGAGCC
TGGGCCTGAC
CCCCAACTTC
AAGAGCAACT
TCGACCTGGC
CGAGGATGCC
AAACTGCAGC
TGAGCAAGGA
CACCTACGAC
GACGACCTGG
ACAACCTGCT
GGCCCAGATC
GGCGACCAGT
ACGCCGACCT
GTTTCTGGCC
GCCAAGAACC
TGTCCGACGC
CATCCTGCTG
AGCGACATCC
TGAGAGTGAA
CACCGAGATC
ACCAAGGCCC
CCCTGAGCGC
CTCTATGATC
AAGAGATACG
ACGAGCACCA
CCAGGACCTG
ACCCTGCTGA
AAGCTCTCGT
GCGGCAGCAG
CTGCCTGAGA
AGTACAAAGA
GATTTTCTTC
GACCAGAGCA
AGAACGGCTA
CGCCGGCTAC
ATTGACGGCG
GAGCCAGCCA
GGAAGAGTTC
TACAAGTTCA
TCAAGCCCAT
CCTGGAAAAG
ATGGACGGCA
CCGAGGAACT
GCTCGTGAAG
CTGAACAGAG
AGGACCTGCT
GCGGAAGCAG
CGGACCTTCG
ACAACGGCAG
CATCCCCCAC
CAGATCCACC
TGGGAGAGCT
GCACGCCATT
CTGCGGCGGC
AGGAAGATTT
TTACCCATTC
CTGAAGGACA
ACCGGGAAAA
GATCGAGAAG
ATCCTGACCT
TCCGCATCCC
CTACTACGTG
GGCCCTCTGG
CCAGGGGAAA
CAGCAGATTC
GCCTGGATGA
CCAGAAAGAG
CGAGGAAACC
ATCACCCCCT
GGAACTTCGA
GGAAGTGGTG
GACAAGGGCG
CTTCCGCCCA
GAGCTTCATC
GAGCGGATGA
CCAACTTCGA
TAAGAACCTG
CCCAACGAGA
AGGTGCTGCC
CAAGCACAGC
CTGCTGTACG
AGTACTTCAC
CGTGTATAAC
GAGCTGACCA
AAGTGAAATA
CGTGACCGAG
GGAATGAGAA
AGCCCGCCTT
CCTGAGCGGC
GAGCAGAAAA
AGGCCATCGT
GGACCTGCTG
TTCAAGACCA
ACCGGAAAGT
GACCGTGAAG
CAGCTGAAAG
AGGACTACTT
CAAGAAAATC
GAGTGCTTCG
ACTCCGTGGA
AATCTCCGGC
GTGGAAGATC
GGTTCAACGC
CTCCCTGGGC
ACATACCACG
ATCTGCTGAA
AATTATCAAG
GACAAGGACT
TCCTGGACAA
TGAGGAAAAC
GAGGACATTC
TGGAAGATAT
CGTGCTGACC
CTGACACTGT
TTGAGGACAG
AGAGATGATC
GAGGAACGGC
TGAAAACCTA
TGCCCACCTG
TTCGACGACA
AAGTGATGAA
GCAGCTGAAG
CGGCGGAGAT
ACACCGGCTG
GGGCAGGCTG
AGCCGGAAGC
TGATCAACGG
CATCCGGGAC
AAGCAGTCCG
GCAAGACAAT
CCTGGATTTC
CTGAAGTCCG
ACGGCTTCGC
CAACAGAAAC
TTCATGCAGC
TGATCCACGA
CGACAGCCTG
ACCTTTAAAG
AGGACATCCA
GAAAGCCCAG
GTGTCCGGCC
AGGGCGATAG
CCTGCACGAG
CACATTGCCA
ATCTGGCCGG
CAGCCCCGCC
ATTAAGAAGG
GCATCCTGCA
GACAGTGAAG
GTGGTGGACG
AGCTCGTGAA
AGTGATGGGC
CGGCACAAGC
CCGAGAACAT
CGTGATCGAA
ATGGCCAGAG
AGAACCAGAC
CACCCAGAAG
GGACAGAAGA
ACAGCCGCGA
GAGAATGAAG
CGGATCGAAG
AGGGCATCAA
AGAGCTGGGC
AGCCAGATCC
TGAAAGAACA
CCCCGTGGAA
AACACCCAGC
TGCAGAACGA
GAAGCTGTAC
CTGTACTACC
TGCAGAATGG
GCGGGATATG
TACGTGGACC
AGGAACTGGA
CATCAACCGG
CTGTCCGACT
ACGATGTGGA
CCATATCGTG
CCTCAGAGCT
TTCTGAAGGA
CGACTCCATC
GACAACAAGG
TGCTGACCAG
AAGCGACAAG
AACCGGGGCA
AGAGCGACAA
CGTGCCCTCC
GAAGAGGTCG
TGAAGAAGAT
GAAGAACTAC
TGGCGGCAGC
TGCTGAACGC
CAAGCTGATT
ACCCAGAGAA
AGTTCGACAA
TCTGACCAAG
GCCGAGAGAG
GCGGCCTGAG
CGAACTGGAT
AAGGCCGGCT
TCATCAAGAG
ACAGCTGGTG
GAAACCCGGC
AGATCACAAA
GCACGTGGCA
CAGATCCTGG
ACTCCCGGAT
GAACACTAAG
TACGACGAGA
ATGACAAGCT
GATCCGGGAA
GTGAAAGTGA
TCACCCTGAA
GTCCAAGCTG
GTGTCCGATT
TCCGGAAGGA
TTTCCAGTTT
TACAAAGTGC
GCGAGATCAA
CAACTACCAC
CACGCCCACG
ACGCCTACCT
GAACGCCGTC
GTGGGAACCG
CCCTGATCAA
AAAGTACCCT
AAGCTGGAAA
GCGAGTTCGT
GTACGGCGAC
TACAAGGTGT
ACGACGTGCG
GAAGATGATC
GCCAAGAGCG
AGCAGGAAAT
CGGCAAGGCT
ACCGCCAAGT
ACTTCTTCTA
CAGCAACATC
ATGAACTTTT
TCAAGACCGA
GATTACCCTG
GCCAACGGCG
AGATCCGGAA
GCGGCCTCTG
ATCGAGACAA
ACGGCGAAAC
CGGGGAGATC
GTGTGGGATA
AGGGCCGGGA
TTTTGCCACC
GTGCGGAAAG
TGCTGAGCAT
GCCCCAAGTG
AATATCGTGA
AAAAGACCGA
GGTGCAGACA
GGCGGCTTCA
GCAAAGAGTC
TATCCTGCCC
AAGAGGAACA
GCGATAAGCT
GATCGCCAGA
AAGAAGGACT
GGGACCCTAA
GAAGTACGGC
GGCTTCGACA
GCCCCACCGT
GGCCTATTCT
GTGCTGGTGG
TGGCCAAAGT
GGAAAAGGGC
AAGTCCAAGA
AACTGAAGAG
TGTGAAAGAG
CTGCTGGGGA
TCACCATCAT
GGAAAGAAGC
AGCTTCGAGA
AGAATCCCAT
CGACTTTCTG
GAAGCCAAGG
GCTACAAAGA
AGTGAAAAAG
GACCTGATCA
TCAAGCTGCC
TAAGTACTCC
CTGTTCGAGC
TGGAAAACGG
CCGGAAGAGA
ATGCTGGCCT
CTGCCGGCGA
ACTGCAGAAG
GGAAACGAAC
TGGCCCTGCC
CTCCAAATAT
GTGAACTTCC
TGTACCTGGC
CAGCCACTAT
GAGAAGCTGA
AGGGCTCCCC
CGAGGATAAT
GAGCAGAAAC
AGCTGTTTGT
GGAACAGCAC
AAGCACTACC
TGGACGAGAT
CATCGAGCAG
ATCAGCGAGT
TCTCCAAGAG
AGTGATCCTG
GCCGACGCTA
ATCTGGACAA
AGTGCTGTCC
GCCTACAACA
AGCACCGGGA
TAAGCCCATC
AGAGAGCAGG
CCGAGAATAT
CATCCACCTG
TTTACCCTGA
CCAATCTGGG
AGCCCCTGCC
GCCTTCAAGT
ACTTTGACAC
CACCATCGAC
CGGAAGAGGT
ACACCAGCAC
CAAAGAGGTG
CTGGACGCCA
CCCTGATCCA
CCAGAGCATC
ACCGGCCTGT
ACGAGACACG
GATCGACCTG
TCTCAGCTGG
GAGGCGACAA
GCGTCCTGCT
GCTACTAAGA
AAGCTGGTCA
AGCTAAGAAA
AAGAAAGCTA
GCGGCAGCGG
CGCCACCAAC
TTCAGCCTGC
TGAAGCAGGC
CGGCGACGTG
GAGGAGAACC
CCGGCCCCAT
GGTGTCTAAG
GGCGAAGAGC
TGTTCACCGG
GGTGGTGCCC
ATCCTGGTCG
AGCTGGACGG
CGACGTAAAC
GGCCACAAGT
TCAGCGTGTC
CGGCGAGGGC
GAGGGCGATG
CCACCTACGG
CAAGCTGACC
CTGAAGTTCA
TCTGCACCAC
CGGCAAGCTG
CCCGTGCCCT
GGCCCACCCT
CGTGACCACC
CTGACCTACG
GCGTGCAGTG
CTTCAGCCGC
TACCCCGACC
ACATGAAGCA
GCACGACTTC
TTCAAGTCCG
CCATGCCCGA
AGGCTACGTC
CAGGAGCGCA
CCATCTTCTT
CAAGGACGAC
GGCAACTACA
AGACCCGCGC
CGAGGTGAAG
TTCGAGGGCG
ACACCCTGGT
GAACCGCATC
GAGCTGAAGG
GCATCGACTT
CAAGGAGGAC
GGCAACATCC
TGGGGCACAA
GCTGGAGTAC
AACTACAACA
GCCACAACGT
CTATATCATG
GCCGACAAGC
AGAAGAACGG
CATCAAGGTG
AACTTCAAGA
TCCGCCACAA
CATCGAGGAC
GGCAGCGTGC
AGCTCGCCGA
CCACTACCAG
CAGAACACCC
CCATCGGCGA
CGGCCCCGTG
CTGCTGCCCG
ACAACCACTA
CCTGAGCACC
CAGTCCGCCC
TGAGCAAAGA
CCCCAACGAG
AAGCGCGATC
ACATGGTCCT
GCTGGAGTTC
GTGACCGCCG
CCGGGATCAC
TCTCGGCATG
GACGAGCTGT
ACAAGTAAAC
GCGTTAAGTC
GACAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAAT
CATCGTCCTT
TCCTTGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCGTCGACTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
GACAAGATCT
GCTTTTTGCT
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGTA
CGTATAGTAG
TTCATGTCAT
CTTATTATTC
AGTATTTATA
ACTTGCAAAG
AAATGAATAT
CAGAGAGTGA
GAGGAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGGCTCT
AGCTATCCCG
CCCCTAACTC
CGCCCATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCGGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGGGACGT
ACCCAATTCG
CCCTATAGTG
AGTCGTATTA
CGCGCGCTCA
CTGGCCGTCG
TTTTACAACG
TCGTGACTGG
GAAAACCCTG
GCGTTACCCA
ACTTAATCGC
CTTGCAGCAC
ATCCCCCTTT
CGCCAGCTGG
CGTAATAGCG
AAGAGGCCCG
CACCGATCGC
CCTTCCCAAC
AGTTGCGCAG
CCTGAATGGC
GAATGGGACG
CGCCCTGTAG
CGGCGCATTA
AGCGCGGCGG
GTGTGGTGGT
TACGCGCAGC
GTGACCGCTA
CACTTGCCAG
CGCCCTAGCG
CCCGCTCCTT
TCGCTTTCTT
CCCTTCCTTT
CTCGCCACGT
TCGCCGGCTT
TCCCCGTCAA
GCTCTAAATC
GGGGGCTCCC
TTTAGGGTTC
CGATTTAGTG
CTTTACGGCA
CCTCGACCCC
AAAAAACTTG
ATTAGGGTGA
TGGTTCACGT
AGTGGGCCAT
CGCCCTGATA
GACGGTTTTT
CGCCCTTTGA
CGTTGGAGTC
CACGTTCTTT
AATAGTGGAC
TCTTGTTCCA
AACTGGAACA
ACACTCAACC
CTATCTCGGT
CTATTCTTTT
GATTTATAAG
GGATTTTGCC
GATTTCGGCC
TATTGGTTAA
AAAATGAGCT
GATTTAACAA
AAATTTAACG
CGAATTTTAA
CAAAATATTA
ACGCTTACAA
TTTAGGTGGC
ACTTTTCGGG
GAAATGTGCG
CGGAACCCCT
ATTTGTTTAT
TTTTCTAAAT
ACATTCAAAT
ATGTATCCGC
TCATGAGACA
ATAACCCTGA
TAAATGCTTC
AATAATATTG
AAAAAGGAAG
AGTATGAGTA
TTCAACATTT
CCGTGTCGCC
CTTATTCCCT
TTTTTGCGGC
ATTTTGCCTT
CCTGTTTTTG
CTCACCCAGA
AACGCTGGTG
AAAGTAAAAG
ATGCTGAAGA
TCAGTTGGGT
GCACGAGTGG
GTTACATCGA
ACTGGATCTC
AACAGCGGTA
AGATCCTTGA
GAGTTTTCGC
CCCGAAGAAC
GTTTTCCAAT
GATGAGCACT
TTTAAAGTTC
TGCTATGTGG
CGCGGTATTA
TCCCGTATTG
ACGCCGGGCA
AGAGCAACTC
GGTCGCCGCA
TACACTATTC
TCAGAATGAC
TTGGTTGAGT
ACTCACCAGT
CACAGAAAAG
CATCTTACGG
ATGGCATGAC
AGTAAGAGAA
TTATGCAGTG
CTGCCATAAC
CATGAGTGAT
AACACTGCGG
CCAACTTACT
TCTGACAACG
ATCGGAGGAC
CGAAGGAGCT
AACCGCTTTT
TTGCACAACA
TGGGGGATCA
TGTAACTCGC
CTTGATCGTT
GGGAACCGGA
GCTGAATGAA
GCCATACCAA
ACGACGAGCG
TGACACCACG
ATGCCTGTAG
CAATGGCAAC
AACGTTGCGC
AAACTATTAA
CTGGCGAACT
ACTTACTCTA
GCTTCCCGGC
AACAATTAAT
AGACTGGATG
GAGGCGGATA
AAGTTGCAGG
ACCACTTCTG
CGCTCGGCCC
TTCCGGCTGG
CTGGTTTATT
GCTGATAAAT
CTGGAGCCGG
TGAGCGTGGG
TCTCGCGGTA
TCATTGCAGC
ACTGGGGCCA
GATGGTAAGC
CCTCCCGTAT
CGTAGTTATC
TACACGACGG
GGAGTCAGGC
AACTATGGAT
GAACGAAATA
GACAGATCGC
TGAGATAGGT
GCCTCACTGA
TTAAGCATTG
GTAACTGTCA
GACCAAGTTT
ACTCATATAT
ACTTTAGATT
GATTTAAAAC
TTCATTTTTA
ATTTAAAAGG
ATCTAGGTGA
AGATCCTTTT
TGATAATCTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TTCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGTTCTTTC
CTGCGTTATC
CCCTGATTCT
GTGGATAACC
GTATTACCGC
CTTTGAGTGA
GCTGATACCG
CTCGCCGCAG
CCGAACGACC
GAGCGCAGCG
AGTCAGTGAG
CGAGGAAGCG
GAAGAGCGCC
CAATACGCAA
ACCGCCTCTC
CCCGCGCGTT
GGCCGATTCA
TTAATGCAGC
TGGCACGACA
GGTTTCCCGA
CTGGAAAGCG
GGCAGTGAGC
GCAACGCAAT
TAATGTGAGT
TAGCTCACTC
ATTAGGCACC
CCAGGCTTTA
CACTTTATGC
TTCCGGCTCG
TATGTTGTGT
GGAATTGTGA
GCGGATAACA
ATTTCACACA
GGAAACAGCT
ATGACCATGA
TTACGCCAAG
CGCGCAATTA
ACCCTCACTA
AAGGGAACAA
AAGCTGGAGC
TGCAAGC