名称: | pHDE-AtU6-26-ALKBH10B(arabidopsis)-sgRNA1-mCherry-Cas9 |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 拟南芥 |
原核抗性: | Spe |
筛选标记: | Hyg |
荧光标记: | 红色 |
启动子: | 35S |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTTTACCCGC
CAATATATCC
TGTCAAACAC
TGATAGTTTA
AACTAGAGTG
TGCTGGAATT
GCCCTTAAGC
TTCGTTGAAC
AACGGAAACT
CGACTTGCCT
TCCGCACAAT
ACATCATTTC
TTCTTAGCTT
TTTTTCTTCT
TCTTCGTTCA
TACAGTTTTT
TTTTGTTTAT
CAGCTTACAT
TTTCTTGAAC
CGTAGCTTTC
GTTTTCTTCT
TTTTAACTTT
CCATTCGGAG
TTTTTGTATC
TTGTTTCATA
GTTTGTCCCA
GGATTAGAAT
GATTAGGCAT
CGAACCTTCA
AGAATTTGAT
TGAATAAAAC
ATCTTCATTC
TTAAGATATG
AAGATAATCT
TCAAAAGGCC
CCTGGGAATC
TGAAAGAAGA
GAAGCAGGCC
CATTTATATG
GGAAAGAACA
ATAGTATTTC
TTATATAGGC
CCATTTAAGT
TGAAAACAAT
CTTCAAAAGT
CCCACATCGC
TTAGATAAGA
AAACGAAGCT
GAGTTTATAT
ACAGCTAGAG
TCGAAGTAGT
GATTGGGATC
GATCTGTCTG
GCGTGCGTTT
TAGAGCTAGA
AATAGCAAGT
TAAAATAAGG
CTAGTCCGTT
ATCAACTTGA
AAAAGTGGCA
CCGAGTCGGT
GCTTTTTTTG
TCCCTTCGAA
GGGCAATTCT
GCAGATATCC
ATCACACTGG
TGAAGTGAAG
CTTGGTCTCA
ACTATGAGAC
CTAAGCTGGC
ACAACTATAT
TTCCAACATC
ACTAGCTACC
ATCAAAAGAT
TGACTTCTCA
TCTTACTCGA
TTGAAACCAA
ATTAACATAG
GGTTTTTATT
TAAATAAAAG
TTTAACCTTC
TTTTTAAAAA
ATTGTTCATA
GTGTCATGTC
AGAACAAGAG
CTACAAATCA
CACATAGCAT
GCATAAGCGG
AGCTATGATG
AGTGGTATTG
TTTTGTTCGT
CACTTGTCAC
TCTTTTCCAA
CACATAATCC
CGACAACAAC
GTAAGAGCAT
CTCTCTCTCT
CCACACACAC
TCATGCATGC
ATGCATTCTT
ACACGTGATT
GCCATGCAAA
TCTCCTTTCT
CACCTATAAA
TACAAACCAA
CCCTTCACTA
CACTCTTCAC
TCAAACCAAA
ACAAGAAAAC
ATACACAAAT
AGCAAAACGG
TACCAACAAT
GGATAACATG
GCCATCATCA
AGGAGTTCAT
GCGCTTCAAG
GTGCACATGG
AGGGCTCCGT
GAACGGCCAC
GAGTTCGAGA
TCGAGGGCGA
GGGCGAGGGC
CGCCCCTACG
AGGGCACCCA
GACCGCCAAG
CTGAAGGTGA
CCAAGGGTGG
CCCCCTGCCC
TTCGCCTGGG
ACATCCTGTC
CCCTCAGTTC
ATGTACGGCT
CCAAGGCCTA
CGTGAAGCAC
CCCGCCGACA
TCCCCGACTA
CTTGAAGCTG
TCCTTCCCCG
AGGGCTTCAA
GTGGGAGCGC
GTGATGAACT
TCGAGGACGG
CGGCGTGGTG
ACCGTGACCC
AGGACTCCTC
CCTGCAGGAC
GGCGAGTTCA
TCTACAAGGT
GAAGCTGCGC
GGCACCAACT
TCCCCTCCGA
CGGCCCCGTA
ATGCAGAAGA
AGACCATGGG
CTGGGAGGCC
TCCTCCGAGC
GGATGTACCC
CGAGGACGGC
GCCCTGAAGG
GCGAGATCAA
GCAGAGGCTG
AAGCTGAAGG
ACGGCGGCCA
CTACGACGCT
GAGGTCAAGA
CCACCTACAA
GGCCAAGAAG
CCCGTGCAGC
TGCCCGGCGC
CTACAACGTC
AACATCAAGT
TGGACATCAC
CTCCCACAAC
GAGGACTACA
CCATCGTGGA
ACAGTACGAA
CGCGCCGAGG
GCCGCCACTC
CACCGGCGGC
ATGGACGAGC
TGTACAAGTG
AGAGCTCTTG
GACTCCCATG
TTGGCAAAGG
CAACCAAACA
AACAATGAAT
GATCCGCTCC
TGCATATGGG
GCGGTTTGAG
TATTTCAACT
GCCATTTGGG
CTGAATTGAA
GACATGCTCC
TGTCAGAAAT
TCCGTGATCT
TACTCAATAT
TCAGTAATCT
CGGCCAATAT
CCTAAATGTG
CGTGGCTTTA
TCTGTCTTTG
TATTGTTTCA
TCAATTCATG
TAACGTTTGC
TTTTCTTATG
AATTTTCAAA
TAAATTATCG
TATTGAGACC
GAATTCTGTC
TAGACCTAAC
TGAGTAAGCT
AGCTTGTTCG
AGTATTATGG
CATTGGGAAA
ACTGTTTTTC
TTGTACCATT
TGTTGTGCTT
GTAATTTACT
GTGTTTTTTA
TTCGGTTTTC
GCTATCGAAC
TGTGAAATGG
AAATGGATGG
AGAAGAGTTA
ATGAATGATA
TGGTCCTTTT
GTTCATTCTC
AAATTAATAT
TATTTGTTTT
TTCTCTTATT
TGTTGTGTGT
TGAATTTGAA
ATTATAAGAG
ATATGCAAAC
ATTTTGTTTT
GAGTAAAAAT
GTGTCAAATC
GTGGCCTCTA
ATGACCGAAG
TTAATATGAG
GAGTAAAACA
CTAGATCCCC
AAACAAGCTT
GAAACTGAAG
GCGGGAAACG
ACAATCTGAT
CATGAGCGGA
GAATTAAGGG
AGTCACGTTA
TGACCCCCGC
CGATGACGCG
GGACAAGCCG
TTTTACGTTT
GGAACTGACA
GAACCGCAAC
GTTGAAGGAG
CCACTCAGCC
GCGGGTTTCT
GGAGTTTAAT
GAGCTAAGCA
CATACGTCAG
AAACCATTAT
TGCGCGTTCA
AAAGTCGCCT
AAGGTCACTA
TCAGCTAGCA
AATATTTCTT
GTCAAAAATG
CTCCACTGAC
GTTCCATAAA
TTCCCCTCGG
TATCCAATTA
GAGTCTCATA
TTCACTCTCA
ATCCAAATAA
TCTGCACCGG
ATCCGCTAGA
GGATCTCGAC
CTGCAAGATC
CCGGGGGGCA
ATGAGATATG
AAAAAGCCTG
AACTCACCGC
GACGTCTGTC
GAGAAGTTTC
TGATCGAAAA
GTTCGACAGC
GTCTCCGACC
TGATGCAGCT
CTCGGAGGGC
GAAGAATCTC
GTGCTTTCAG
CTTCGATATA
GGAGGGCGTG
GATATGTCCT
GCGGGTAAAT
AGCTGCGCCG
ATGGTTTCTA
CAAAGATCGT
TATGTTTATC
GGCACTTTGC
ATCGGCCGCG
CTCCCGATTC
CGGAAGTGCT
TGACATTGGG
GAATTCAGCG
AGAGCCTGAC
CTATTGCATC
TCCCGCCGTG
CACAGGGTGT
CACGTTGCAA
GACCTGCCTG
AAACCGAACT
GCCCGCTGTT
CTGCAGCCGG
TCGCGGAGGC
CATGGATGCG
ATCGCTGCGG
CCGATCTTAG
CCAGACGAGC
GGGTTCGGCC
CATTCGGACC
GCAAGGAATC
GGTCAATACA
CTACATGGCG
TGATTTCATA
TGCGCGATTG
CTGATCCCCA
TGTGTATCAC
TGGCAAACTG
TGATGGACGA
CACCGTCAGT
GCGTCCGTCG
CGCAGGCTCT
CGATGAGCTG
ATGCTTTGGG
CCGAGGACTG
CCCCGAAGTC
CGGCACCTCG
TGCACGCGGA
TTTCGGCTCC
AACAATGTCC
TGACGGACAA
TGGCCGCATA
ACAGCGGTCA
TTGACTGGAG
CGAGGCGATG
TTCGGGGATT
CCCAATACGA
GGTCGCCAAC
ATCTTCTTCT
GGAGGCCGTG
GTTGGCTTGT
ATGGAGCAGC
AGACGCGCTA
CTTCGAGCGG
AGGCATCCGG
AGCTTGCAGG
ATCGCCGCGG
CTCCGGGCGT
ATATGCTCCG
CATTGGTCTT
GACCAACTCT
ATCAGAGCTT
GGTTGACGGC
AATTTCGATG
ATGCAGCTTG
GGCGCAGGGT
CGATGCGACG
CAATCGTCCG
ATCCGGAGCC
GGGACTGTCG
GGCGTACACA
AATCGCCCGC
AGAAGCGCGG
CCGTCTGGAC
CGATGGCTGT
GTAGAAGTAC
TCGCCGATAG
TGGAAACCGA
CGCCCCAGCA
CTCGTCCGGG
ATCTTGGAGG
TGATGTAACA
TGATCACAAG
CTGATCCCCC
GAATTTCCCC
GATCGTTCAA
ACATTTGGCA
ATAAAGTTTC
TTAAGATTGA
ATCCTGTTGC
CGGTCTTGCG
ATGATTATCA
TATAATTTCT
GTTGAATTAC
GTTAAGCATG
TAATAATTAA
CATGTAATGC
ATGACGTTAT
TTATGAGATG
GGTTTTTATG
ATTAGAGTCC
CGCAATTATA
CATTTAATAC
GCGATAGAAA
ACAAAATATA
GCGCGCAAAC
TAGGATAAAT
TATCGCGCGC
GGTGTCATCT
ATGTTACTAG
ATCGGGAATT
GATCCCCCCT
CGACAGCTTG
CATGCCAGCT
TGGGCTGCAG
GTCGACGCGT
CAACATGGTG
GAGCACGACA
CTCTCGTCTA
CTCCAAGAAT
ATCAAAGATA
CAGTCTCAGA
AGACCAAAGG
GCTATTGAGA
CTTTTCAACA
AAGGGTAATA
TCGGGAAACC
TCCTCGGATT
CCATTGCCCA
GCTATCTGTC
ACTTCATCAA
AAGGACAGTA
GAAAAGGAAG
GTGGCACCTA
CAAATGCCAT
CATTGCGATA
AAGGAAAGGC
TATCGTTCAA
GATGCCTCTG
CCGACAGTGG
TCCCAAAGAT
GGACCCCCAC
CCACGAGGAG
CATCGTGGAA
AAAGAAGACG
TTCCAACCAC
GTCTTCAAAG
CAAGTGGATT
GATGTGATAT
CTCCACTGAC
GTAAGGGATG
ACGCACAATC
CCACTATCCT
TCGCAAGACC
CTTCCTCTAT
ATAAGGAAGT
TCATTTCATT
TGGAGAGGAC
ACGATATGGA
CAAGAAGTAC
TCCATTGGGC
TCGATATCGG
CACAAACAGC
GTCGGCTGGG
CCGTCATTAC
GGACGAGTAC
AAGGTGCCGA
GCAAAAAATT
CAAAGTTCTG
GGCAATACCG
ATCGCCACAG
CATAAAGAAG
AACCTCATTG
GCGCCCTCCT
GTTCGACTCC
GGGGAGACGG
CCGAAGCCAC
GCGGCTCAAA
AGAACAGCAC
GGCGCAGATA
TACCCGCAGA
AAGAATCGGA
TCTGCTACCT
GCAGGAGATC
TTTAGTAATG
AGATGGCTAA
GGTGGATGAC
TCTTTCTTCC
ATAGGCTGGA
GGAGTCCTTT
TTGGTGGAGG
AGGATAAAAA
GCACGAGCGC
CACCCAATCT
TTGGCAATAT
CGTGGACGAG
GTGGCGTACC
ATGAAAAGTA
CCCAACCATA
TATCATCTGA
GGAAGAAGCT
TGTAGACAGT
ACTGATAAGG
CTGACTTGCG
GTTGATCTAT
CTCGCGCTGG
CGCATATGAT
CAAATTTCGG
GGACACTTCC
TCATCGAGGG
GGACCTGAAC
CCAGACAACA
GCGATGTCGA
CAAACTCTTT
ATCCAACTGG
TTCAGACTTA
CAATCAGCTT
TTCGAAGAGA
ACCCGATCAA
CGCATCCGGA
GTTGACGCCA
AAGCAATCCT
GAGCGCTAGG
CTGTCCAAAT
CCCGGCGGCT
CGAAAACCTC
ATCGCACAGC
TCCCTGGGGA
GAAGAAGAAC
GGCCTGTTTG
GTAATCTTAT
CGCCCTGTCA
CTCGGGCTGA
CCCCCAACTT
TAAATCTAAC
TTCGACCTGG
CCGAAGATGC
CAAGCTTCAA
CTGAGCAAAG
ACACCTACGA
TGATGATCTC
GACAATCTGC
TGGCCCAGAT
CGGCGACCAG
TACGCAGACC
TTTTTTTGGC
GGCAAAGAAC
CTGTCAGACG
CCATTCTGCT
GAGTGATATT
CTGCGAGTGA
ACACGGAGAT
CACCAAAGCT
CCGCTGAGCG
CTAGTATGAT
CAAGCGCTAT
GATGAGCACC
ACCAAGACTT
GACTTTGCTG
AAGGCCCTTG
TCAGACAGCA
ACTGCCTGAG
AAGTACAAGG
AAATTTTCTT
CGATCAGTCT
AAAAATGGCT
ACGCCGGATA
CATTGACGGC
GGAGCAAGCC
AGGAGGAATT
TTACAAATTT
ATTAAGCCCA
TCTTGGAAAA
AATGGACGGC
ACCGAGGAGC
TGCTGGTAAA
GCTTAACAGA
GAAGATCTGT
TGCGCAAACA
GCGCACTTTC
GACAATGGAA
GCATCCCCCA
CCAGATTCAC
CTGGGCGAAC
TGCACGCTAT
CCTCAGGCGG
CAAGAGGATT
TCTACCCCTT
TTTGAAAGAT
AACAGGGAAA
AGATTGAGAA
AATCCTCACA
TTTCGGATAC
CCTACTATGT
AGGCCCCCTC
GCCCGGGGAA
ATTCCAGATT
CGCGTGGATG
ACTCGCAAAT
CAGAAGAGAC
CATCACTCCC
TGGAACTTCG
AGGAAGTCGT
GGATAAGGGG
GCCTCTGCCC
AGTCCTTCAT
CGAAAGGATG
ACTAACTTTG
ATAAAAATCT
GCCTAACGAA
AAGGTGCTTC
CTAAACACTC
TCTGCTGTAC
GAGTACTTCA
CAGTTTATAA
CGAGCTCACC
AAGGTCAAAT
ACGTCACAGA
AGGGATGAGA
AAGCCAGCAT
TCCTGTCTGG
AGAGCAGAAG
AAAGCTATCG
TGGACCTCCT
CTTCAAGACG
AACCGGAAAG
TTACCGTGAA
ACAGCTCAAA
GAAGACTATT
TCAAAAAGAT
TGAATGTTTC
GACTCTGTTG
AAATCAGCGG
AGTGGAGGAT
CGCTTCAACG
CATCCCTGGG
AACGTATCAC
GATCTCCTGA
AAATCATTAA
AGACAAGGAC
TTCCTGGACA
ATGAGGAGAA
CGAGGACATT
CTTGAGGACA
TTGTCCTCAC
CCTTACGTTG
TTTGAAGATA
GGGAGATGAT
TGAAGAACGC
TTGAAAACTT
ACGCTCATCT
CTTCGACGAC
AAAGTCATGA
AACAGCTCAA
GAGGCGCCGA
TATACAGGAT
GGGGGCGGCT
GTCAAGAAAA
CTGATCAATG
GGATCCGAGA
CAAGCAGAGT
GGAAAGACAA
TCCTGGATTT
TCTTAAGTCC
GATGGATTTG
CCAACCGGAA
CTTCATGCAG
TTGATCCATG
ATGACTCTCT
CACCTTTAAG
GAGGACATCC
AGAAAGCACA
AGTTTCTGGC
CAGGGGGACA
GTCTTCACGA
GCACATCGCT
AATCTTGCAG
GTAGCCCAGC
TATCAAAAAG
GGAATACTGC
AGACCGTTAA
GGTCGTGGAT
GAACTCGTCA
AAGTAATGGG
AAGGCATAAG
CCCGAGAATA
TCGTTATCGA
GATGGCCCGA
GAGAACCAAA
CTACCCAGAA
GGGACAGAAG
AACAGTAGGG
AAAGGATGAA
GAGGATTGAA
GAGGGTATAA
AAGAACTGGG
GTCCCAAATC
CTTAAGGAAC
ACCCAGTTGA
AAACACCCAG
CTTCAGAATG
AGAAGCTCTA
CCTGTACTAC
CTGCAGAACG
GCAGGGACAT
GTACGTGGAT
CAGGAACTGG
ACATCAATCG
GCTCTCCGAC
TACGACGTGG
ATCATATCGT
GCCCCAGTCT
TTTCTCAAAG
ATGATTCTAT
TGATAATAAA
GTGTTGACAA
GATCCGATAA
AAATAGAGGG
AAGAGTGATA
ACGTCCCCTC
AGAAGAAGTT
GTCAAGAAAA
TGAAAAATTA
TTGGCGGCAG
CTGCTGAACG
CCAAACTGAT
CACACAACGG
AAGTTCGATA
ATCTGACTAA
GGCTGAACGA
GGTGGCCTGT
CTGAGTTGGA
TAAAGCCGGC
TTCATCAAAA
GGCAGCTTGT
TGAGACACGC
CAGATCACCA
AGCACGTGGC
CCAAATTCTC
GATTCACGCA
TGAACACCAA
GTACGATGAA
AATGACAAAC
TGATTCGAGA
GGTGAAAGTT
ATTACTCTGA
AGTCTAAGCT
GGTCTCAGAT
TTCAGAAAGG
ACTTTCAGTT
TTATAAGGTG
AGAGAGATCA
ACAATTACCA
CCATGCGCAT
GATGCCTACC
TGAATGCAGT
GGTAGGCACT
GCACTTATCA
AAAAATATCC
CAAGCTTGAA
TCTGAATTTG
TTTACGGAGA
CTATAAAGTG
TACGATGTTA
GGAAAATGAT
CGCAAAGTCT
GAGCAGGAAA
TAGGCAAGGC
CACCGCTAAG
TACTTCTTTT
ACAGCAATAT
TATGAATTTT
TTCAAGACCG
AGATTACACT
GGCCAATGGA
GAGATTCGGA
AGCGACCACT
TATCGAAACA
AACGGAGAAA
CAGGAGAAAT
CGTGTGGGAC
AAGGGTAGGG
ATTTCGCGAC
AGTCCGGAAG
GTCCTGTCCA
TGCCGCAGGT
GAACATCGTT
AAAAAGACCG
AAGTACAGAC
CGGAGGCTTC
TCCAAGGAAA
GTATCCTCCC
GAAAAGGAAC
AGCGACAAGC
TGATCGCACG
CAAAAAAGAT
TGGGACCCCA
AGAAATACGG
CGGATTCGAT
TCTCCTACAG
TCGCTTACAG
TGTACTGGTT
GTGGCCAAAG
TGGAGAAAGG
GAAGTCTAAA
AAACTCAAAA
GCGTCAAGGA
ACTGCTGGGC
ATCACAATCA
TGGAGCGATC
AAGCTTCGAA
AAAAACCCCA
TCGACTTTCT
CGAGGCGAAA
GGATATAAAG
AGGTCAAAAA
AGACCTCATC
ATTAAGCTTC
CCAAGTACTC
TCTCTTTGAG
CTTGAAAACG
GCCGGAAACG
AATGCTCGCT
AGTGCGGGCG
AGCTGCAGAA
AGGTAACGAG
CTGGCACTGC
CCTCTAAATA
CGTTAATTTC
TTGTATCTGG
CCAGCCACTA
TGAAAAGCTC
AAAGGGTCTC
CCGAAGATAA
TGAGCAGAAG
CAGCTGTTCG
TGGAACAACA
CAAACACTAC
CTTGATGAGA
TCATCGAGCA
AATAAGCGAA
TTCTCCAAAA
GAGTGATCCT
CGCCGACGCT
AACCTCGATA
AGGTGCTTTC
TGCTTACAAT
AAGCACAGGG
ATAAGCCCAT
CAGGGAGCAG
GCAGAAAACA
TTATCCACTT
GTTTACTCTG
ACCAACTTGG
GCGCGCCTGC
AGCCTTCAAG
TACTTCGACA
CCACCATAGA
CAGAAAGCGG
TACACCTCTA
CAAAGGAGGT
CCTGGACGCC
ACACTGATTC
ATCAGTCAAT
TACGGGGCTC
TATGAAACAA
GAATCGACCT
CTCTCAGCTC
GGTGGAGACA
GCAGGGCTGA
CCCCAAGAAG
AAGAGGAAGG
TGTGATCGAT
CCAGGCCTCC
CAGCTTTCGT
CCGTATCATC
GGTTTCGACA
ACGTTCGTCA
AGTTCAATGC
ATCAGTTTCA
TTGCCCACAC
ACCAGAATCC
TACTAAGTTT
GAGTATTATG
GCATTGGAAA
AGCTGTTTTC
TTCTATCATT
TGTTCTGCTT
GTAATTTACT
GTGTTCTTTC
AGTTTTTGTT
TTCGGACATC
AAAATGCAAA
TGGATGGATA
AGAGTTAATA
AATGATATGG
TCCTTTTGTT
CATTCTCAAA
TTATTATTAT
CTGTTGTTTT
TACTTTAATG
GGTTGAATTT
AAGTAAGAAA
GGAACTAACA
GTGTGATATT
AAGGTGCAAT
GTTAGACATA
TAAAACAGTC
TTTCACCTCT
CTTTGGTTAT
GTCTTGAATT
GGTTTGTTTC
TTCACTTATC
TGTGTAATCA
AGTTTACTAT
GAGTCTATGA
TCAAGTAATT
ATGCAATCAA
GTTAAGTACA
GTATAGGCTT
TTTGTGTCGA
GGGGGTACCG
AGTCGAGGAA
TTCACTGGCC
GTCGTTTTAC
AACGTCGTGA
CTGGGAAAAC
CCTGGCGTTA
CCCAACTTAA
TCGCCTTGCA
GCACATCCCC
CTTTCGCCAG
CTGGCGTAAT
AGCGAAGAGG
CCCGCACCGA
TCGCCCTTCC
CAACAGTTGC
GCAGCCTGAA
TGGCGGGTAC
CGAGCTCGAA
TTCAATTCGG
CGTTAATTCA
GTACATTAAA
AACGTCCGCA
ATGTGTTATT
AAGTTGTCTA
AGCGTCAATT
TGTTTACACC
ACAATATATC
CTGCCACCAG
CCAGCCAACA
GCTCCCCGAC
CGGCAGCTCG
GCACAAAATC
ACCACTCGAT
ACAGGCAGCC
CATCAGTCCG
GGACGGCGTC
AGCGGGAGAG
CCGTTGTAAG
GCGGCAGACT
TTGCTCATGT
TACCGATGCT
ATTCGGAAGA
ACGGCAACTA
AGCTGCCGGG
TTTGAAACAC
GGATGATCTC
GCGGAGGGTA
GCATGTTGAT
TGTAACGATG
ACAGAGCGTT
GCTGCCTGTG
ATCAATTCGG
GCACGAACCC
AGTGGACATA
AGCCTCGTTC
GGTTCGTAAG
CTGTAATGCA
AGTAGCGTAA
CTGCCGTCAC
GCAACTGGTC
CAGAACCTTG
ACCGAACGCA
GCGGTGGTAA
CGGCGCAGTG
GCGGTTTTCA
TGGCTTCTTG
TTATGACATG
TTTTTTTGGG
GTACAGTCTA
TGCCTCGGGC
ATCCAAGCAG
CAAGCGCGTT
ACGCCGTGGG
TCGATGTTTG
ATGTTATGGA
GCAGCAACGA
TGTTACGCAG
CAGGGCAGTC
GCCCTAAAAC
AAAGTTAAAC
ATCATGGGGG
AAGCGGTGAT
CGCCGAAGTA
TCGACTCAAC
TATCAGAGGT
AGTTGGCGTC
ATCGAGCGCC
ATCTCGAACC
GACGTTGCTG
GCCGTACATT
TGTACGGCTC
CGCAGTGGAT
GGCGGCCTGA
AGCCACACAG
TGATATTGAT
TTGCTGGTTA
CGGTGACCGT
AAGGCTTGAT
GAAACAACGC
GGCGAGCTTT
GATCAACGAC
CTTTTGGAAA
CTTCGGCTTC
CCCTGGAGAG
AGCGAGATTC
TCCGCGCTGT
AGAAGTCACC
ATTGTTGTGC
ACGACGACAT
CATTCCGTGG
CGTTATCCAG
CTAAGCGCGA
ACTGCAATTT
GGAGAATGGC
AGCGCAATGA
CATTCTTGCA
GGTATCTTCG
AGCCAGCCAC
GATCGACATT
GATCTGGCTA
TCTTGCTGAC
AAAAGCAAGA
GAACATAGCG
TTGCCTTGGT
AGGTCCAGCG
GCGGAGGAAC
TCTTTGATCC
GGTTCCTGAA
CAGGATCTAT
TTGAGGCGCT
AAATGAAACC
TTAACGCTAT
GGAACTCGCC
GCCCGACTGG
GCTGGCGATG
AGCGAAATGT
AGTGCTTACG
TTGTCCCGCA
TTTGGTACAG
CGCAGTAACC
GGCAAAATCG
CGCCGAAGGA
TGTCGCTGCC
GACTGGGCAA
TGGAGCGCCT
GCCGGCCCAG
TATCAGCCCG
TCATACTTGA
AGCTAGACAG
GCTTATCTTG
GACAAGAAGA
AGATCGCTTG
GCCTCGCGCG
CAGATCAGTT
GGAAGAATTT
GTCCACTACG
TGAAAGGCGA
GATCACCAAG
GTAGTCGGCA
AATAATGTCT
AGCTAGAAAT
TCGTTCAAGC
CGACGCCGCT
TCGCCGGCGT
TAACTCAAGC
GATTAGATGC
ACTAAGCACA
TAATTGCTCA
CAGCCAAACT
ATCAGGTCAA
GTCTGCTTTT
ATTATTTTTA
AGCGTGCATA
ATAAGCCCTA
CACAAATTGG
GAGATATATC
ATGCATGACC
AAAATCCCTT
AACGTGAGTT
TTCGTTCCAC
TGAGCGTCAG
ACCCCGTAGA
AAAGATCAAA
GGATCTTCTT
GAGATCCTTT
TTTTCTGCGC
GTAATCTGCT
GCTTGCAAAC
AAAAAAACCA
CCGCTACCAG
CGGTGGTTTG
TTTGCCGGAT
CAAGAGCTAC
CAACTCTTTT
TCCGAAGGTA
ACTGGCTTCA
GCAGAGCGCA
GATACCAAAT
ACTGTCCTTC
TAGTGTAGCC
GTAGTTAGGC
CACCACTTCA
AGAACTCTGT
AGCACCGCCT
ACATACCTCG
CTCTGCTAAT
CCTGTTACCA
GTGGCTGCTG
CCAGTGGCGA
TAAGTCGTGT
CTTACCGGGT
TGGACTCAAG
ACGATAGTTA
CCGGATAAGG
CGCAGCGGTC
GGGCTGAACG
GGGGGTTCGT
GCACACAGCC
CAGCTTGGAG
CGAACGACCT
ACACCGAACT
GAGATACCTA
CAGCGTGAGC
TATGAGAAAG
CGCCACGCTT
CCCGAAGGGA
GAAAGGCGGA
CAGGTATCCG
GTAAGCGGCA
GGGTCGGAAC
AGGAGAGCGC
ACGAGGGAGC
TTCCAGGGGG
AAACGCCTGG
TATCTTTATA
GTCCTGTCGG
GTTTCGCCAC
CTCTGACTTG
AGCGTCGATT
TTTGTGATGC
TCGTCAGGGG
GGCGGAGCCT
ATGGAAAAAC
GCCAGCAACG
CGGCCTTTTT
ACGGTTCCTG
GCCTTTTGCT
GGCCTTTTGC
TCACATGTTC
TTTCCTGCGT
TATCCCCTGA
TTCTGTGGAT
AACCGTATTA
CCGCCTTTGA
GTGAGCTGAT
ACCGCTCGCC
GCAGCCGAAC
GACCGAGCGC
AGCGAGTCAG
TGAGCGAGGA
AGCGGAAGAG
CGCCTGATGC
GGTATTTTCT
CCTTACGCAT
CTGTGCGGTA
TTTCACACCG
CATATGGTGC
ACTCTCAGTA
CAATCTGCTC
TGATGCCGCA
TAGTTAAGCC
AGTATACACT
CCGCTATCGC
TACGTGACTG
GGTCATGGCT
GCGCCCCGAC
ACCCGCCAAC
ACCCGCTGAC
GCGCCCTGAC
GGGCTTGTCT
GCTCCCGGCA
TCCGCTTACA
GACAAGCTGT
GACCGTCTCC
GGGAGCTGCA
TGTGTCAGAG
GTTTTCACCG
TCATCACCGA
AACGCGCGAG
GCAGGGTGCC
TTGATGTGGG
CGCCGGCGGT
CGAGTGGCGA
CGGCGCGGCT
TGTCCGCGCC
CTGGTAGATT
GCCTGGCCGT
AGGCCAGCCA
TTTTTGAGCG
GCCAGCGGCC
GCGATAGGCC
GACGCGAAGC
GGCGGGGCGT
AGGGAGCGCA
GCGACCGAAG
GGTAGGCGCT
TTTTGCAGCT
CTTCGGCTGT
GCGCTGGCCA
GACAGTTATG
CACAGGCCAG
GCGGGTTTTA
AGAGTTTTAA
TAAGTTTTAA
AGAGTTTTAG
GCGGAAAAAT
CGCCTTTTTT
CTCTTTTATA
TCAGTCACTT
ACATGTGTGA
CCGGTTCCCA
ATGTACGGCT
TTGGGTTCCC
AATGTACGGG
TTCCGGTTCC
CAATGTACGG
CTTTGGGTTC
CCAATGTACG
TGCTATCCAC
AGGAAAGAGA
CCTTTTCGAC
CTTTTTCCCC
TGCTAGGGCA
ATTTGCCCTA
GCATCTGCTC
CGTACATTAG
GAACCGGCGG
ATGCTTCGCC
CTCGATCAGG
TTGCGGTAGC
GCATGACTAG
GATCGGGCCA
GCCTGCCCCG
CCTCCTCCTT
CAAATCGTAC
TCCGGCAGGT
CATTTGACCC
GATCAGCTTG
CGCACGGTGA
AACAGAACTT
CTTGAACTCT
CCGGCGCTGC
CACTGCGTTC
GTAGATCGTC
TTGAACAACC
ATCTGGCTTC
TGCCTTGCCT
GCGGCGCGGC
GTGCCAGGCG
GTAGAGAAAA
CGGCCGATGC
CGGGATCGAT
CAAAAAGTAA
TCGGGGTGAA
CCGTCAGCAC
GTCCGGGTTC
TTGCCTTCTG
TGATCTCGCG
GTACATCCAA
TCAGCTAGCT
CGATCTCGAT
GTACTCCGGC
CGCCCGGTTT
CGCTCTTTAC
GATCTTGTAG
CGGCTAATCA
AGGCTTCACC
CTCGGATACC
GTCACCAGGC
GGCCGTTCTT
GGCCTTCTTC
GTACGCTGCA
TGGCAACGTG
CGTGGTGTTT
AACCGAATGC
AGGTTTCTAC
CAGGTCGTCT
TTCTGCTTTC
CGCCATCGGC
TCGCCGGCAG
AACTTGAGTA
CGTCCGCAAC
GTGTGGACGG
AACACGCGGC
CGGGCTTGTC
TCCCTTCCCT
TCCCGGTATC
GGTTCATGGA
TTCGGTTAGA
TGGGAAACCG
CCATCAGTAC
CAGGTCGTAA
TCCCACACAC
TGGCCATGCC
GGCCGGCCCT
GCGGAAACCT
CTACGTGCCC
GTCTGGAAGC
TCGTAGCGGA
TCACCTCGCC
AGCTCGTCGG
TCACGCTTCG
ACAGACGGAA
AACGGCCACG
TCCATGATGC
TGCGACTATC
GCGGGTGCCC
ACGTCATAGA
GCATCGGAAC
GAAAAAATCT
GGTTGCTCGT
CGCCCTTGGG
CGGCTTCCTA
ATCGACGGCG
CACCGGCTGC
CGGCGGTTGC
CGGGATTCTT
TGCGGATTCG
ATCAGCGGCC
GCTTGCCACG
ATTCACCGGG
GCGTGCTTCT
GCCTCGATGC
GTTGCCGCTG
GGCGGCCTGC
GCGGCCTTCA
ACTTCTCCAC
CAGGTCATCA
CCCAGCGCCG
CGCCGATTTG
TACCGGGCCG
GATGGTTTGC
GACCGTCACG
CCGATTCCTC
GGGCTTGGGG
GTTCCAGTGC
CATTGCAGGG
CCGGCAGACA
ACCCAGCCGC
TTACGCCTGG
CCAACCGCCC
GTTCCTCCAC
ACATGGGGCA
TTCCACGGCG
TCGGTGCCTG
GTTGTTCTTG
ATTTTCCATG
CCGCCTCCTT
TAGCCGCTAA
AATTCATCTA
CTCATTTATT
CATTTGCTCA
TTTACTCTGG
TAGCTGCGCG
ATGTATTCAG
ATAGCAGCTC
GGTAATGGTC
TTGCCTTGGC
GTACCGCGTA
CATCTTCAGC
TTGGTGTGAT
CCTCCGCCGG
CAACTGAAAG
TTGACCCGCT
TCATGGCTGG
CGTGTCTGCC
AGGCTGGCCA
ACGTTGCAGC
CTTGCTGCTG
CGTGCGCTCG
GACGGCCGGC
ACTTAGCGTG
TTTGTGCTTT
TGCTCATTTT
CTCTTTACCT
CATTAACTCA
AATGAGTTTT
GATTTAATTT
CAGCGGCCAG
CGCCTGGACC
TCGCGGGCAG
CGTCGCCCTC
GGGTTCTGAT
TCAAGAACGG
TTGTGCCGGC
GGCGGCAGTG
CCTGGGTAGC
TCACGCGCTG
CGTGATACGG
GACTCAAGAA
TGGGCAGCTC
GTACCCGGCC
AGCGCCTCGG
CAACCTCACC
GCCGATGCGC
GTGCCTTTGA
TCGCCCGCGA
CACGACAAAG
GCCGCTTGTA
GCCTTCCATC
CGTGACCTCA
ATGCGCTGCT
TAACCAGCTC
CACCAGGTCG
GCGGTGGCCC
ATATGTCGTA
AGGGCTTGGC
TGCACCGGAA
TCAGCACGAA
GTCGGCTGCC
TTGATCGCGG
ACACAGCCAA
GTCCGCCGCC
TGGGGCGCTC
CGTCGATCAC
TACGAAGTCG
CGCCGGCCGA
TGGCCTTCAC
GTCGCGGTCA
ATCGTCGGGC
GGTCGATGCC
GACAACGGTT
AGCGGTTGAT
CTTCCCGCAC
GGCCGCCCAA
TCGCGGGCAC
TGCCCTGGGG
ATCGGAATCG
ACTAACAGAA
CATCGGCCCC
GGCGAGTTGC
AGGGCGCGGG
CTAGATGGGT
TGCGATGGTC
GTCTTGCCTG
ACCCGCCTTT
CTGGTTAAGT
ACAGCGATAA
CCTTCATGCG
TTCCCCTTGC
GTATTTGTTT
ATTTACTCAT
CGCATCATAT
ACGCAGCGAC
CGCATGACGC
AAGCTGTTTT
ACTCAAATAC
ACATCACCTT
TTTAGACGGC
GGCGCTCGGT
TTCTTCAGCG
GCCAAGCTGG
CCGGCCAGGC
CGCCAGCTTG
GCATCAGACA
AACCGGCCAG
GATTTCATGC
AGCCGCACGG
TTGAGACGTG
CGCGGGCGGC
TCGAACACGT
ACCCGGCCGC
GATCATCTCC
GCCTCGATCT
CTTCGGTAAT
GAAAAACGGT
TCGTCCTGGC
CGTCCTGGTG
CGGTTTCATG
CTTGTTCCTC
TTGGCGTTCA
TTCTCGGCGG
CCGCCAGGGC
GTCGGCCTCG
GTCAATGCGT
CCTCACGGAA
GGCACCGCGC
CGCCTGGCCT
CGGTGGGCGT
CACTTCCTCG
CTGCGCTCAA
GTGCGCGGTA
CAGGGTCGAG
CGATGCACGC
CAAGCAGTGC
AGCCGCCTCT
TTCACGGTGC
GGCCTTCCTG
GTCGATCAGC
TCGCGGGCGT
GCGCGATCTG
TGCCGGGGTG
AGGGTAGGGC
GGGGGCCAAA
CTTCACGCCT
CGGGCCTTGG
CGGCCTCGCG
CCCGCTCCGG
GTGCGGTCGA
TGATTAGGGA
ACGCTCGAAC
TCGGCAATGC
CGGCGAACAC
GGTCAACACC
ATGCGGCCGG
CCGGCGTGGT
GGTGTCGGCC
CACGGCTCTG
CCAGGCTACG
CAGGCCCGCG
CCGGCCTCCT
GGATGCGCTC
GGCAATGTCC
AGTAGGTCGC
GGGTGCTGCG
GGCCAGGCGG
TCTAGCCTGG
TCACTGTCAC
AACGTCGCCA
GGGCGTAGGT
GGTCAAGCAT
CCTGGCCAGC
TCCGGGCGGT
CGCGCCTGGT
GCCGGTGATC
TTCTCGGAAA
ACAGCTTGGT
GCAGCCGGCC
GCGTGCAGTT
CGGCCCGTTG
GTTGGTCAAG
TCCTGGTCGT
CGGTGCTGAC
GCGGGCATAG
CCCAGCAGGC
CAGCGGCGGC
GCTCTTGTTC
ATGGCGTAAT
GTCTCCGGTT
CTAGTCGCAA
GTATTCTACT
TTATGCGACT
AAAACACGCG
ACAAGAAAAC
GCCAGGAAAA
GGGCAGGGCG
GCAGCCTGTC
GCGTAACTTA
GGACTTGTGC
GACATGTCGT
TTTCAGAAGA
CGGCTGCACT
GAACGTCAGA
AGCCGACTGC
ACTATAGCAG
CGGAGGGGTT
GGATCAAAGT
ACTTTAAAGT
ACTTTAAAGT
ACTTTAAAGT
ACTTTGATCC
CGAGGGGAAC
CCTGTGGTTG
GCATGCACAT
ACAAATGGAC
GAACGGATAA
ACCTTTTCAC
GCCCTTTTAA
ATATCCGATT
ATTCTAATAA
ACGCTCTTTT
CTCTTAG