名称: | pC0056 - LwCas13a-msfGFP-NES |
---|---|
别称: | Plasmid#105815 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGTTGAC
ATTGATTATT
GACTAGTTAT
TAATAGTAAT
CAATTACGGG
GTCATTAGTT
CATAGCCCAT
ATATGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGCGC
GTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
CTGATCTTCA
GACCTGGAGG
AGGAGATATG
AGGGACAATT
GGAGAAGTGA
ATTATATAAA
TATAAAGTAG
TAAAAATTGA
ACCATTAGGA
GTAGCACCCA
CCAAGGCAAA
GAGAAGAGTG
GTGCAGAGAG
AAAAAAGAGC
AGTGGGAATA
GGAGCTTTGT
TCCTTGGGTT
CTTGGGAGCA
GCAGGAAGCA
CTATGGGCGC
AGCGTCAATG
ACGCTGACGG
TACAGGCCAG
ACAATTATTG
TCTGGTATAG
TGCAGCAGCA
GAACAATTTG
CTGAGGGCTA
TTGAGGCGCA
ACAGCATCTG
TTGCAACTCA
CAGTCTGGGG
CATCAAGCAG
CTCCAGGCAA
GAATCCTGGC
TGTGGAAAGA
TACCTAAAGG
ATCAACAGCT
CCTGGGGATT
TGGGGTTGCT
CTGGAAAACT
CATTTGCACC
ACTGCTGTGC
CTTGGAATGC
TAGTTGGAGT
AATAAATCTC
TGGAACAGAT
TTGGAATCAC
ACGACCTGGA
TGGAGTGGGA
CAGAGAAATT
AACAATTACA
CAAGCTTAAT
ACACTCCTTA
ATTGAAGAAT
CGCAAAACCA
GCAAGAAAAG
AATGAACAAG
AATTATTGGA
ATTAGATAAA
TGGGCAAGTT
TGTGGAATTG
GTTTAACATA
ACAAATTGGC
TGTGGTATAT
AAAATTATTC
ATAATGATAG
TAGGAGGCTT
GGTAGGTTTA
AGAATAGTTT
TTGCTGTACT
TTCTATAGTG
AATAGAGTTA
GGCAGGGATA
TTCACCATTA
TCGTTTCAGA
CCCACCTCCC
AACCCCGAGG
GGACCCGACA
GGCCCGAAGG
AATAGAAGAA
GAAGGTGGAG
AGAGAGACAG
AGACAGATCC
ATTCGATTAG
TGAACGGATC
GGCACTGCGT
GCGCCAATTC
TGCAGACAAA
TGGCAGTATT
CATCCACAAT
TTTAAAAGAA
AAGGGGGGAT
TGGGGGGTAC
AGTGCAGGGG
AAAGAATAGT
AGACATAATA
GCAACAGACA
TACAAACTAA
AGAATTACAA
AAACAAATTA
CAAAAATTCA
AAATTTTCGG
GTTTATTACA
GGGACAGCAG
AGATCCAGTT
TGGTTAATTA
ATGCAAAGAT
GGATAAAGTT
TTAAACAGAG
AGGAATCTTT
GCAGCTAATG
GACCTTCTAG
GTCTTGAAAG
GAGTGGGAAT
TGGCTCCGGT
GCCCGTCAGT
GGGCAGAGCG
CACATCGCCC
ACAGTCCCCG
AGAAGTTGGG
GGGAGGGGTC
GGCAATTGAA
CCGGTGCCTA
GAGAAGGTGG
CGCGGGGTAA
ACTGGGAAAG
TGATGTCGTG
TACTGGCTCC
GCCTTTTTCC
CGAGGGTGGG
GGAGAACCGT
ATATAAGTGC
AGTAGTCGCC
GTGAACGTTC
TTTTTCGCAA
CGGGTTTGCC
GCCAGAACAC
AGGTAAGTGC
CGTGTGTGGT
TCCCGCGGGC
CTGGCCTCTT
TACGGGTTAT
GGCCCTTGCG
TGCCTTGAAT
TACTTCCACC
TGGCTGCAGT
ACGTGATTCT
TGATCCCGAG
CTTCGGGTTG
GAAGTGGGTG
GGAGAGTTCG
AGGCCTTGCG
CTTAAGGAGC
CCCTTCGCCT
CGTGCTTGAG
TTGAGGCCTG
GCCTGGGCGC
TGGGGCCGCC
GCGTGCGAAT
CTGGTGGCAC
CTTCGCGCCT
GTCTCGCTGC
TTTCGATAAG
TCTCTAGCCA
TTTAAAATTT
TTGATGACCT
GCTGCGACGC
TTTTTTTCTG
GCAAGATAGT
CTTGTAAATG
CGGGCCAAGA
TCTGCACACT
GGTATTTCGG
TTTTTGGGGC
CGCGGGCGGC
GACGGGGCCC
GTGCGTCCCA
GCGCACATGT
TCGGCGAGGC
GGGGCCTGCG
AGCGCGGCCA
CCGAGAATCG
GACGGGGGTA
GTCTCAAGCT
GGCCGGCCTG
CTCTGGTGCC
TGGCCTCGCG
CCGCCGTGTA
TCGCCCCGCC
CTGGGCGGCA
AGGCTGGCCC
GGTCGGCACC
AGTTGCGTGA
GCGGAAAGAT
GGCCGCTTCC
CGGCCCTGCT
GCAGGGAGCT
CAAAATGGAG
GACGCGGCGC
TCGGGAGAGC
GGGCGGGTGA
GTCACCCACA
CAAAGGAAAA
GGGCCTTTCC
GTCCTCAGCC
GTCGCTTCAT
GTGACTCCAC
GGAGTACCGG
GCGCCGTCCA
GGCACCTCGA
TTAGTTCTCG
AGCTTTTGGA
GTACGTCGTC
TTTAGGTTGG
GGGGAGGGGT
TTTATGCGAT
GGAGTTTCCC
CACACTGAGT
GGGTGGAGAC
TGAAGTTAGG
CCAGCTTGGC
ACTTGATGTA
ATTCTCCTTG
GAATTTGCCC
TTTTTGAGTT
TGGATCTTGG
TTCATTCTCA
AGCCTCAGAC
AGTGGTTCAA
AGTTTTTTTC
TTCCATTTCA
GGTGTCGTGA
CGTACGGCCA
CCATGCTGTA
CCCTGAGCGG
CTGCGGCGGA
TCCTGACCGG
ATCCATGAAA
GTGACCAAGG
TCGACGGCAT
CAGCCACAAG
AAGTACATCG
AAGAGGGCAA
GCTCGTGAAG
TCCACCAGCG
AGGAAAACCG
GACCAGCGAG
AGACTGAGCG
AGCTGCTGAG
CATCCGGCTG
GACATCTACA
TCAAGAACCC
CGACAACGCC
TCCGAGGAAG
AGAACCGGAT
CAGAAGAGAG
AACCTGAAGA
AGTTCTTTAG
CAACAAGGTG
CTGCACCTGA
AGGACAGCGT
GCTGTATCTG
AAGAACCGGA
AAGAAAAGAA
CGCCGTGCAG
GACAAGAACT
ATAGCGAAGA
GGACATCAGC
GAGTACGACC
TGAAAAACAA
GAACAGCTTC
TCCGTGCTGA
AGAAGATCCT
GCTGAACGAG
GACGTGAACT
CTGAGGAACT
GGAAATCTTT
CGGAAGGACG
TGGAAGCCAA
GCTGAACAAG
ATCAACAGCC
TGAAGTACAG
CTTCGAAGAG
AACAAGGCCA
ACTACCAGAA
GATCAACGAG
AACAACGTGG
AAAAAGTGGG
CGGCAAGAGC
AAGCGGAACA
TCATCTACGA
CTACTACAGA
GAGAGCGCCA
AGCGCAACGA
CTACATCAAC
AACGTGCAGG
AAGCCTTCGA
CAAGCTGTAT
AAGAAAGAGG
ATATCGAGAA
ACTGTTTTTC
CTGATCGAGA
ACAGCAAGAA
GCACGAGAAG
TACAAGATCC
GCGAGTACTA
TCACAAGATC
ATCGGCCGGA
AGAACGACAA
AGAGAACTTC
GCCAAGATTA
TCTACGAAGA
GATCCAGAAC
GTGAACAACA
TCAAAGAGCT
GATTGAGAAG
ATCCCCGACA
TGTCTGAGCT
GAAGAAAAGC
CAGGTGTTCT
ACAAGTACTA
CCTGGACAAA
GAGGAACTGA
ACGACAAGAA
TATTAAGTAC
GCCTTCTGCC
ACTTCGTGGA
AATCGAGATG
TCCCAGCTGC
TGAAAAACTA
CGTGTACAAG
CGGCTGAGCA
ACATCAGCAA
CGATAAGATC
AAGCGGATCT
TCGAGTACCA
GAATCTGAAA
AAGCTGATCG
AAAACAAACT
GCTGAACAAG
CTGGACACCT
ACGTGCGGAA
CTGCGGCAAG
TACAACTACT
ATCTGCAAGT
GGGCGAGATC
GCCACCTCCG
ACTTTATCGC
CCGGAACCGG
CAGAACGAGG
CCTTCCTGAG
AAACATCATC
GGCGTGTCCA
GCGTGGCCTA
CTTCAGCCTG
AGGAACATCC
TGGAAACCGA
GAACGAGAAC
GGTATCACCG
GCCGGATGCG
GGGCAAGACC
GTGAAGAACA
ACAAGGGCGA
AGAGAAATAC
GTGTCCGGCG
AGGTGGACAA
GATCTACAAT
GAGAACAAGC
AGAACGAAGT
GAAAGAAAAT
CTGAAGATGT
TCTACAGCTA
CGACTTCAAC
ATGGACAACA
AGAACGAGAT
CGAGGACTTC
TTCGCCAACA
TCGACGAGGC
CATCAGCAGC
ATCAGACACG
GCATCGTGCA
CTTCAACCTG
GAACTGGAAG
GCAAGGACAT
CTTCGCCTTC
AAGAATATCG
CCCCCAGCGA
GATCTCCAAG
AAGATGTTTC
AGAACGAAAT
CAACGAAAAG
AAGCTGAAGC
TGAAAATCTT
CAAGCAGCTG
AACAGCGCCA
ACGTGTTCAA
CTACTACGAG
AAGGATGTGA
TCATCAAGTA
CCTGAAGAAT
ACCAAGTTCA
ACTTCGTGAA
CAAAAACATC
CCCTTCGTGC
CCAGCTTCAC
CAAGCTGTAC
AACAAGATTG
AGGACCTGCG
GAATACCCTG
AAGTTTTTTT
GGAGCGTGCC
CAAGGACAAA
GAAGAGAAGG
ACGCCCAGAT
CTACCTGCTG
AAGAATATCT
ACTACGGCGA
GTTCCTGAAC
AAGTTCGTGA
AAAACTCCAA
GGTGTTCTTT
AAGATCACCA
ATGAAGTGAT
CAAGATTAAC
AAGCAGCGGA
ACCAGAAAAC
CGGCCACTAC
AAGTATCAGA
AGTTCGAGAA
CATCGAGAAA
ACCGTGCCCG
TGGAATACCT
GGCCATCATC
CAGAGCAGAG
AGATGATCAA
CAACCAGGAC
AAAGAGGAAA
AGAATACCTA
CATCGACTTT
ATTCAGCAGA
TTTTCCTGAA
GGGCTTCATC
GACTACCTGA
ACAAGAACAA
TCTGAAGTAT
ATCGAGAGCA
ACAACAACAA
TGACAACAAC
GACATCTTCT
CCAAGATCAA
GATCAAAAAG
GATAACAAAG
AGAAGTACGA
CAAGATCCTG
AAGAACTATG
AGAAGCACAA
TCGGAACAAA
GAAATCCCTC
ACGAGATCAA
TGAGTTCGTG
CGCGAGATCA
AGCTGGGGAA
GATTCTGAAG
TACACCGAGA
ATCTGAACAT
GTTTTACCTG
ATCCTGAAGC
TGCTGAACCA
CAAAGAGCTG
ACCAACCTGA
AGGGCAGCCT
GGAAAAGTAC
CAGTCCGCCA
ACAAAGAAGA
AACCTTCAGC
GACGAGTTGG
AACTGATCAA
CCTGCTGAAC
CTGGACAACA
ACAGAGTGAC
CGAGGACTTC
GAGCTGGAAG
CCAACGAGAT
CGGCAAGTTC
CTGGACTTCA
ACGAAAACAA
AATCAAGGAC
CGGAAAGAGC
TGAAAAAGTT
CGACACCAAC
AAGATCTATT
TCGACGGCGA
GAACATCATC
AAGCACCGGG
CCTTCTACAA
TATCAAGAAA
TACGGCATGC
TGAATCTGCT
GGAAAAGATC
GCCGATAAGG
CCAAGTATAA
GATCAGCCTG
AAAGAACTGA
AAGAGTACAG
CAACAAGAAG
AATGAGATTG
AAAAGAACTA
CACCATGCAG
CAGAACCTGC
ACCGGAAGTA
CGCCAGACCC
AAGAAGGACG
AAAAGTTCAA
CGACGAGGAC
TACAAAGAGT
ATGAGAAGGC
CATCGGCAAC
ATCCAGAAGT
ACACCCACCT
GAAGAACAAG
GTGGAATTCA
ATGAGCTGAA
CCTGCTGCAG
GGCCTGCTGC
TGAAGATCCT
GCACCGGCTC
GTGGGCTACA
CCAGCATCTG
GGAGCGGGAC
CTGAGATTCC
GGCTGAAGGG
CGAGTTTCCC
GAGAACCACT
ACATCGAGGA
AATTTTCAAT
TTCGACAACT
CCAAGAATGT
GAAGTACAAA
AGCGGCCAGA
TCGTGGAAAA
GTATATCAAC
TTCTACAAAG
AACTGTACAA
GGACAATGTG
GAAAAGCGGA
GCATCTACTC
CGACAAGAAA
GTGAAGAAAC
TGAAGCAGGA
AAAAAAGGAC
CTGTACATCC
GGAACTACAT
TGCCCACTTC
AACTACATCC
CCCACGCCGA
GATTAGCCTG
CTGGAAGTGC
TGGAAAACCT
GCGGAAGCTG
CTGTCCTACG
ACCGGAAGCT
GAAGAACGCC
ATCATGAAGT
CCATCGTGGA
CATTCTGAAA
GAATACGGCT
TCGTGGCCAC
CTTCAAGATC
GGCGCTGACA
AGAAGATCGA
AATCCAGACC
CTGGAATCAG
AGAAGATCGT
GCACCTGAAG
AATCTGAAGA
AAAAGAAACT
GATGACCGAC
CGGAACAGCG
AGGAACTGTG
CGAACTCGTG
AAAGTCATGT
TCGAGTACAA
GGCCCTGGAA
GGAGGAGGTG
GAAGCGGAGG
AGGAGGAAGC
GGAGGAGGAG
GTAGCGTGAG
TAAAGGTGAA
GAACTCTTCA
CTGGAGTAGT
GCCCATTCTG
GTAGAGCTTG
ATGGAGATGT
AAATGGACAT
AAATTCTCCG
TCAGGGGCGA
AGGCGAAGGG
GACGCCACGA
ATGGTAAGCT
GACTCTGAAA
TTCATCTGTA
CGACGGGCAA
ACTGCCCGTC
CCATGGCCTA
CACTCGTAAC
GACCCTCACC
TACGGCGTGC
AATGCTTTTC
TCGATATCCC
GACCACATGA
AACAGCATGA
CTTTTTCAAG
TCTGCAATGC
CTGAAGGTTA
TGTTCAAGAA
AGGACCATCA
GCTTTAAGGA
TGATGGTACA
TATAAAACCC
GAGCCGAGGT
TAAATTTGAA
GGGGACACTC
TGGTTAATCG
AATTGAACTG
AAAGGTATTG
ATTTTAAGGA
GGACGGTAAC
ATACTGGGGC
ACAAGTTGGA
GTACAACTTT
AACAGCCATA
ATGTGTATAT
TACCGCTGAT
AAGCAGAAAA
ATGGGATAAA
GGCCAACTTT
AAGATCCGAC
ATAATGTCGA
AGATGGTAGT
GTTCAACTGG
CTGATCATTA
CCAACAAAAT
ACGCCCATCG
GAGATGGACC
TGTACTCTTG
CCTGACAATC
ATTATCTCTC
CACGCAATCA
AAGCTTTCCA
AGGACCCAAA
CGAAAAGAGA
GATCACATGG
TCCTTCTGGA
ATTTGTGACT
GCCGCAGGCA
TCACTCTCGG
TATGGATGAG
CTGTACAAGG
GATCCCTGTA
TCCTGAGCGG
CTGCGGCGGA
TCCTGACCTA
CCCATACGAT
GTTCCAGATT
ACGCTTATCC
CTACGACGTG
CCTGATTATG
CATACCCATA
TGATGTCCCC
GACTATGCCT
AAGAATTCGA
TATCAAGCTT
ATCGATAATC
AACCTCTGGA
TTACAAAATT
TGTGAAAGAT
TGACTGGTAT
TCTTAACTAT
GTTGCTCCTT
TTACGCTATG
TGGATACGCT
GCTTTAATGC
CTTTGTATCA
TGCTATTGCT
TCCCGTATGG
CTTTCATTTT
CTCCTCCTTG
TATAAATCCT
GGTTGCTGTC
TCTTTATGAG
GAGTTGTGGC
CCGTTGTCAG
GCAACGTGGC
GTGGTGTGCA
CTGTGTTTGC
TGACGCAACC
CCCACTGGTT
GGGGCATTGC
CACCACCTGT
CAGCTCCTTT
CCGGGACTTT
CGCTTTCCCC
CTCCCTATTG
CCACGGCGGA
ACTCATCGCC
GCCTGCCTTG
CCCGCTGCTG
GACAGGGGCT
CGGCTGTTGG
GCACTGACAA
TTCCGTGGTG
TTGTCGGGGA
AATCATCGTC
CTTTCCTTGG
CTGCTCGCCT
GTGTTGCCAC
CTGGATTCTG
CGCGGGACGT
CCTTCTGCTA
CGTCCCTTCG
GCCCTCAATC
CAGCGGACCT
TCCTTCCCGC
GGCCTGCTGC
CGGCTCTGCG
GCCTCTTCCG
CGTCTTCGCC
TTCGCCCTCA
GACGAGTCGG
ATCTCCCTTT
GGGCCGCCTC
CCCGCATCGA
TACCGTCGAC
CTCGAGACCT
AGAAAAACAT
GGAGCAATCA
CAAGTAGCAA
TACAGCAGCT
ACCAATGCTG
ATTGTGCCTG
GCTAGAAGCA
CAAGAGGAGG
AGGAGGTGGG
TTTTCCAGTC
ACACCTCAGG
TACCTTTAAG
ACCAATGACT
TACAAGGCAG
CTGTAGATCT
TAGCCACTTT
TTAAAAGAAA
AGGGGGGACT
GGAAGGGCTA
ATTCACTCCC
AACGAAGACA
AGATATCCTT
GATCTGTGGA
TCTACCACAC
ACAAGGCTAC
TTCCCTGATT
GGCAGAACTA
CACACCAGGG
CCAGGGATCA
GATATCCACT
GACCTTTGGA
TGGTGCTACA
AGCTAGTACC
AGTTGAGCAA
GAGAAGGTAG
AAGAAGCCAA
TGAAGGAGAG
AACACCCGCT
TGTTACACCC
TGTGAGCCTG
CATGGGATGG
ATGACCCGGA
GAGAGAAGTA
TTAGAGTGGA
GGTTTGACAG
CCGCCTAGCA
TTTCATCACA
TGGCCCGAGA
GCTGCATCCG
GACTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GGGCCCGTTT
AAACCCGCTG
ATCAGCCTCG
ACTGTGCCTT
CTAGTTGCCA
GCCATCTGTT
GTTTGCCCCT
CCCCCGTGCC
TTCCTTGACC
CTGGAAGGTG
CCACTCCCAC
TGTCCTTTCC
TAATAAAATG
AGGAAATTGC
ATCGCATTGT
CTGAGTAGGT
GTCATTCTAT
TCTGGGGGGT
GGGGTGGGGC
AGGACAGCAA
GGGGGAGGAT
TGGGAAGACA
ATAGCAGGCA
TGCTGGGGAT
GCGGTGGGCT
CTATGGCTTC
TGAGGCGGAA
AGAACCAGCT
GGGGCTCTAG
GGGGTATCCC
CACGCGCCCT
GTAGCGGCGC
ATTAAGCGCG
GCGGGTGTGG
TGGTTACGCG
CAGCGTGACC
GCTACACTTG
CCAGCGCCCT
AGCGCCCGCT
CCTTTCGCTT
TCTTCCCTTC
CTTTCTCGCC
ACGTTCGCCG
GCTTTCCCCG
TCAAGCTCTA
AATCGGGGGC
TCCCTTTAGG
GTTCCGATTT
AGTGCTTTAC
GGCACCTCGA
CCCCAAAAAA
CTTGATTAGG
GTGATGGTTC
ACGTAGTGGG
CCATCGCCCT
GATAGACGGT
TTTTCGCCCT
TTGACGTTGG
AGTCCACGTT
CTTTAATAGT
GGACTCTTGT
TCCAAACTGG
AACAACACTC
AACCCTATCT
CGGTCTATTC
TTTTGATTTA
TAAGGGATTT
TGCCGATTTC
GGCCTATTGG
TTAAAAAATG
AGCTGATTTA
ACAAAAATTT
AACGCGAATT
AATTCTGTGG
AATGTGTGTC
AGTTAGGGTG
TGGAAAGTCC
CCAGGCTCCC
CAGCAGGCAG
AAGTATGCAA
AGCATGCATC
TCAATTAGTC
AGCAACCAGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
TAGTCCCGCC
CCTAACTCCG
CCCATCCCGC
CCCTAACTCC
GCCCAGTTCC
GCCCATTCTC
CGCCCCATGG
CTGACTAATT
TTTTTTATTT
ATGCAGAGGC
CGAGGCCGCC
TCTGCCTCTG
AGCTATTCCA
GAAGTAGTGA
GGAGGCTTTT
TTGGAGGCCT
AGGCTTTTGC
AAAAAGCTCC
CGGGAGCTTG
TATATCCATT
TTCGGATCTG
ATCAGCACGT
GTTGACAATT
AATCATCGGC
ATAGTATATC
GGCATAGTAT
AATACGACAA
GGTGAGGAAC
TAAACCATGG
CCAAGTTGAC
CAGTGCCGTT
CCGGTGCTCA
CCGCGCGCGA
CGTCGCCGGA
GCGGTCGAGT
TCTGGACCGA
CCGGCTCGGG
TTCTCCCGGG
ACTTCGTGGA
GGACGACTTC
GCCGGTGTGG
TCCGGGACGA
CGTGACCCTG
TTCATCAGCG
CGGTCCAGGA
CCAGGTGGTG
CCGGACAACA
CCCTGGCCTG
GGTGTGGGTG
CGCGGCCTGG
ACGAGCTGTA
CGCCGAGTGG
TCGGAGGTCG
TGTCCACGAA
CTTCCGGGAC
GCCTCCGGGC
CGGCCATGAC
CGAGATCGGC
GAGCAGCCGT
GGGGGCGGGA
GTTCGCCCTG
CGCGACCCGG
CCGGCAACTG
CGTGCACTTC
GTGGCCGAGG
AGCAGGACTG
ACACGTGCTA
CGAGATTTCG
ATTCCACCGC
CGCCTTCTAT
GAAAGGTTGG
GCTTCGGAAT
CGTTTTCCGG
GACGCCGGCT
GGATGATCCT
CCAGCGCGGG
GATCTCATGC
TGGAGTTCTT
CGCCCACCCC
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GTATACCGTC
GACCTCTAGC
TAGAGCTTGG
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGGATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTGAC